Pub Date : 2022-06-22DOI: 10.1177/08839115221106700
J. Bakshi, M. Mehra, S. Grewal, D. Dhingra, S. Kumari
In the present study, the anti-diabetic and antimicrobial properties of berberine were improved using non-ionic guar gum and ionic acacia gum as nanocarriers. Berberine loaded guar-acacia gum nanocomplexes were synthesized by employing ionic complexation method. The formulation was characterized by dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), Scanning electron microscopy (SEM) and evaluated for in vitro dissolution study, anti-diabetic activity and antimicrobial activity. The optimized berberine loaded guar-acacia gum nanocomplexes had a particle size of 290.2 nm as indicated by DLS and drug entrapment efficiency of 96.5%. Morphological analysis revealed that berberine nanocomplexes were spherical-shaped with a smooth surface and size in the range of 100–250 nm. Moreover, berberine loaded guar-acacia nanocomplexes showed good stability and controlled released property in vitro. Antimicrobial activity against bacterial strains and fungal strains demonstrated the higher antimicrobial potential of berberine loaded gum nanocomplexes than gum nanocomplexes (blank) and pure berberine as indicated by the greater zone of inhibition diameter. In vitro anti-diabetic assessment showed higher percentage inhibition of the α-amylase enzyme by berberine loaded gum nanocomplexes as compared to pure berberine and blank nanocomplexes. In conclusion, the improved biological potency of berberine upon encapsulation into gum nanocomplexes indicates that berberine loaded guar-acacia gum nanocomplexes can be used as a promising candidate against diabetes and pathogenic microorganisms in the near future.
{"title":"Synthesis, characterization and evaluation of in vitro antimicrobial and anti-diabetic activity of berberine encapsulated in guar-acacia gum nanocomplexes","authors":"J. Bakshi, M. Mehra, S. Grewal, D. Dhingra, S. Kumari","doi":"10.1177/08839115221106700","DOIUrl":"https://doi.org/10.1177/08839115221106700","url":null,"abstract":"In the present study, the anti-diabetic and antimicrobial properties of berberine were improved using non-ionic guar gum and ionic acacia gum as nanocarriers. Berberine loaded guar-acacia gum nanocomplexes were synthesized by employing ionic complexation method. The formulation was characterized by dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), Scanning electron microscopy (SEM) and evaluated for in vitro dissolution study, anti-diabetic activity and antimicrobial activity. The optimized berberine loaded guar-acacia gum nanocomplexes had a particle size of 290.2 nm as indicated by DLS and drug entrapment efficiency of 96.5%. Morphological analysis revealed that berberine nanocomplexes were spherical-shaped with a smooth surface and size in the range of 100–250 nm. Moreover, berberine loaded guar-acacia nanocomplexes showed good stability and controlled released property in vitro. Antimicrobial activity against bacterial strains and fungal strains demonstrated the higher antimicrobial potential of berberine loaded gum nanocomplexes than gum nanocomplexes (blank) and pure berberine as indicated by the greater zone of inhibition diameter. In vitro anti-diabetic assessment showed higher percentage inhibition of the α-amylase enzyme by berberine loaded gum nanocomplexes as compared to pure berberine and blank nanocomplexes. In conclusion, the improved biological potency of berberine upon encapsulation into gum nanocomplexes indicates that berberine loaded guar-acacia gum nanocomplexes can be used as a promising candidate against diabetes and pathogenic microorganisms in the near future.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74463747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-17DOI: 10.1177/08839115221104072
Lai Suo, Zhijun Xue, Puyu Wang, Hongshan Wu, Yao Chen, Jing Shen
Oral and maxillofacial tumors, trauma and infections are the main causes of jaw defects, whose clinical treatment is very complicated. With the development of biological tissue engineering, many biological materials have been widely used in various fields of stomatology, and they play a very important role in the repair and replacement of maxillofacial bone defects. In this study, we intended to prepare a graphene oxide/hyaluronic acid/chitosan (GO/HA/CS) composite hydrogel with different mass ratios of GO: 0.1% (0.1% GO/HA/CS), 0.25% (0.25% GO/HA/CS), 0.5% (0.5% GO/HA/CS), and 1% (1% GO/HA/CS), prepare it into a multilayered and stable composite scaffold through 3D-printing technology, observe the surface morphology of the composite scaffold through scanning electron microscopy (SEM), and then test its physical and chemical properties, mechanical properties, water swelling rate, in vitro degradation and other material properties. Moreover, the biological performance of the GO/HA/CS composite scaffold was studied through experiments, such as cell morphology observation, cell adhesion, cell proliferation, and live-dead cell staining. The results showed that through chemical cross-linking and 3D-printing technology, a porous (pore size: 450–580 μm) and multilayered GO/HA/CS biological scaffold could be successfully constructed, and its surface was an interconnected microporous structure, and the porosity decreased (94%−40%) gradually with the increase of GO. Meanwhile, with the change in GO concentration, some mechanical properties of the scaffold could be improved, such as water swelling rate, degradation rate, and elastic modulus. In addition, the composite scaffold with the appropriate amount of GO had almost no cytotoxicity and could promote cell growth and proliferation, especially 0.25% GO/HA/CS composite scaffold. Consequently, the 0.25% GO/HA/CS composite scaffold had excellent biological material properties and good biocompatibility with osteoblasts, which may provide a new idea for the repair of jaw defects.
{"title":"Improvement of osteogenic properties using a 3D-printed graphene oxide/hyaluronic acid/chitosan composite scaffold","authors":"Lai Suo, Zhijun Xue, Puyu Wang, Hongshan Wu, Yao Chen, Jing Shen","doi":"10.1177/08839115221104072","DOIUrl":"https://doi.org/10.1177/08839115221104072","url":null,"abstract":"Oral and maxillofacial tumors, trauma and infections are the main causes of jaw defects, whose clinical treatment is very complicated. With the development of biological tissue engineering, many biological materials have been widely used in various fields of stomatology, and they play a very important role in the repair and replacement of maxillofacial bone defects. In this study, we intended to prepare a graphene oxide/hyaluronic acid/chitosan (GO/HA/CS) composite hydrogel with different mass ratios of GO: 0.1% (0.1% GO/HA/CS), 0.25% (0.25% GO/HA/CS), 0.5% (0.5% GO/HA/CS), and 1% (1% GO/HA/CS), prepare it into a multilayered and stable composite scaffold through 3D-printing technology, observe the surface morphology of the composite scaffold through scanning electron microscopy (SEM), and then test its physical and chemical properties, mechanical properties, water swelling rate, in vitro degradation and other material properties. Moreover, the biological performance of the GO/HA/CS composite scaffold was studied through experiments, such as cell morphology observation, cell adhesion, cell proliferation, and live-dead cell staining. The results showed that through chemical cross-linking and 3D-printing technology, a porous (pore size: 450–580 μm) and multilayered GO/HA/CS biological scaffold could be successfully constructed, and its surface was an interconnected microporous structure, and the porosity decreased (94%−40%) gradually with the increase of GO. Meanwhile, with the change in GO concentration, some mechanical properties of the scaffold could be improved, such as water swelling rate, degradation rate, and elastic modulus. In addition, the composite scaffold with the appropriate amount of GO had almost no cytotoxicity and could promote cell growth and proliferation, especially 0.25% GO/HA/CS composite scaffold. Consequently, the 0.25% GO/HA/CS composite scaffold had excellent biological material properties and good biocompatibility with osteoblasts, which may provide a new idea for the repair of jaw defects.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90295419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-10DOI: 10.1177/08839115221104074
Linli Li, Fengjuan Wang
Anethum graveolens extract (AGE) is known for its anti-inflammatory, antioxidative, and antibacterial activities. As wound infection, hyperactivity of inflammatory responses, and high oxidative stress are the leading causes of delayed wound healing, we were encouraged to design a delivery vehicle for AGE to develop a potential wound dressing material. In the current study, AGE was incorporated into the polyvinyl alcohol (PVA) scaffolds matrix via the electrospinning method. Various characterization methods were applied to assess the physicochemical and biological properties of the dressings. Cell culture studies with fibroblast cell line showed that AGE-loaded dressings could significantly promote cell viability under normal and oxidative stress conditions. The prepared wound dressings’ wound healing and anti-inflammatory properties were investigated on an excisional injury rat model. Wound healing assay showed that AGE-delivering wound dressings could significantly improve the wound healing response, as evidenced by a significantly higher rate of wound closure, epithelial thickness, and collagen deposition. Gene expression analysis revealed that the produced dressings downregulated inflammation-associated genes such as IL-1β and NFK-β. This preliminary research suggests the potential applicability of AGE-loaded PVA dressings in the clinic.
{"title":"Wound healing and anti-inflammatory effects of Anethum graveolens extract loaded in PVA fibers: An in vitro and in vivo study","authors":"Linli Li, Fengjuan Wang","doi":"10.1177/08839115221104074","DOIUrl":"https://doi.org/10.1177/08839115221104074","url":null,"abstract":"Anethum graveolens extract (AGE) is known for its anti-inflammatory, antioxidative, and antibacterial activities. As wound infection, hyperactivity of inflammatory responses, and high oxidative stress are the leading causes of delayed wound healing, we were encouraged to design a delivery vehicle for AGE to develop a potential wound dressing material. In the current study, AGE was incorporated into the polyvinyl alcohol (PVA) scaffolds matrix via the electrospinning method. Various characterization methods were applied to assess the physicochemical and biological properties of the dressings. Cell culture studies with fibroblast cell line showed that AGE-loaded dressings could significantly promote cell viability under normal and oxidative stress conditions. The prepared wound dressings’ wound healing and anti-inflammatory properties were investigated on an excisional injury rat model. Wound healing assay showed that AGE-delivering wound dressings could significantly improve the wound healing response, as evidenced by a significantly higher rate of wound closure, epithelial thickness, and collagen deposition. Gene expression analysis revealed that the produced dressings downregulated inflammation-associated genes such as IL-1β and NFK-β. This preliminary research suggests the potential applicability of AGE-loaded PVA dressings in the clinic.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77852695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-07DOI: 10.1177/08839115221104071
Simin Nazarnezhad, M. Salehi, H. Samadian, Arian Ehtermi, N. Kasaiyan, H. Khastar, Ghasem Abbaszadeh-Goudarzi, Nariman Rezaei Kolarijani, Hodays Yeganehfard, H. Ziaei
The current study’s main aim was to fabricate and evaluate alginate (Alg) hydrogel containing retinoic acid (RA) as wound healing materials. Different RA concentrations (2, 10, and 50% w/w) were incorporated into the hydrogel. The results showed that the prepared hydrogels had a porous structure with a pore size of 69.69 ± 22.1 µm for pure Alg hydrogel and 78.44 ± 27.8 µm for Alg/RA hydrogel. The swelling measurement showed that the hydrogels swelled up to 65% and the incorporation of RA reduced the degree of swelling . The in vitro studies confirmed the hemo- and biocompatibility of the Alg/RA 2% and increasing the RA concentration induced hemolysis and toxic effects. The animal studies showed that the lowest RA concentration resulted in the best treatment outcome while increasing the RA concentration suppressed the healing process. In conclusion, these results showed that RA induced wound healing process at low concentration, and the prepared hydrogel could be used as the wound healing materials.
{"title":"In vitro and in vivo evaluation of porous alginate hydrogel containing retinoic acid for skin wound healing applications","authors":"Simin Nazarnezhad, M. Salehi, H. Samadian, Arian Ehtermi, N. Kasaiyan, H. Khastar, Ghasem Abbaszadeh-Goudarzi, Nariman Rezaei Kolarijani, Hodays Yeganehfard, H. Ziaei","doi":"10.1177/08839115221104071","DOIUrl":"https://doi.org/10.1177/08839115221104071","url":null,"abstract":"The current study’s main aim was to fabricate and evaluate alginate (Alg) hydrogel containing retinoic acid (RA) as wound healing materials. Different RA concentrations (2, 10, and 50% w/w) were incorporated into the hydrogel. The results showed that the prepared hydrogels had a porous structure with a pore size of 69.69 ± 22.1 µm for pure Alg hydrogel and 78.44 ± 27.8 µm for Alg/RA hydrogel. The swelling measurement showed that the hydrogels swelled up to 65% and the incorporation of RA reduced the degree of swelling . The in vitro studies confirmed the hemo- and biocompatibility of the Alg/RA 2% and increasing the RA concentration induced hemolysis and toxic effects. The animal studies showed that the lowest RA concentration resulted in the best treatment outcome while increasing the RA concentration suppressed the healing process. In conclusion, these results showed that RA induced wound healing process at low concentration, and the prepared hydrogel could be used as the wound healing materials.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87817394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-07DOI: 10.1177/08839115221098058
Phuong Le Thi, T. Tran, H. Luu, Dieu Linh Tran, T. H. Thi, D. Nguyen
Injectable hydrogels offer a wide range of attractive benefits in drug delivery applications, such as non-invasive administration, easy drug incorporation and locally controlled release at the target sites. Herein, we designed a simple and efficient method to prepare injectable hydrogels composed of gelatin and cyclodextrin (CD) for high loading capacity of hydrophobic drugs. The hydrogels were formed by thiol-functionalized gelatin (GSH) and βCD-vinyl sulfone (βCD-VS) as cross-linker, via thiol-ene “click” chemistry. Hydrogels comprising of different cross-linker feed amount were investigated in terms of their physico-chemical properties, such as gelation time, mechanical strength, swelling ratio, porosity and degradation rates. For the use as a drug delivery vehicle, dexamethasone (DEX), a commonly anti-inflammatory, immunosuppressive but poorly water soluble drug was chosen to show the high drug loading capacity and prolonged drug release of hydrogels. The drug release was found to be depended on the concentration of βCD-VS due to the drug-CD interaction. In vitro cytotoxicity experiment also showed the cell compatibility of these hydrogels against human dermal fibroblasts. In summary, we expect this gelatin-CD “click” hydrogel will be a promising candidate for localized and long-term delivery of hydrophobic drugs. Graphical Abstract
可注射水凝胶在药物递送应用中提供了广泛的吸引力，例如非侵入性给药，易于药物合并和在目标部位局部控制释放。本研究设计了一种简单、高效的方法制备由明胶和环糊精(CD)组成的可注射型水凝胶，以提高疏水药物的负载能力。以巯基功能化明胶(GSH)为交联剂，以β cd -乙烯基砜(βCD-VS)为交联剂，通过巯基“咔嗒”反应形成水凝胶。研究了不同交联剂投加量的水凝胶的理化性质，如凝胶时间、机械强度、溶胀率、孔隙率和降解率。地塞米松(dexamethasone, DEX)是一种常见的抗炎、免疫抑制但水溶性较差的药物，具有较高的载药量和较长的水凝胶释药时间。由于药物- cd相互作用，药物释放依赖于βCD-VS的浓度。体外细胞毒性实验也显示了水凝胶对人真皮成纤维细胞的细胞相容性。总之，我们预计这种明胶- cd“点击”水凝胶将成为一种有希望的局部和长期递送疏水药物的候选者。图形抽象
{"title":"In situ forming gelatin: Cyclodextrin hydrogels prepared by “click chemistry” to improve the sustained release of hydrophobic drugs","authors":"Phuong Le Thi, T. Tran, H. Luu, Dieu Linh Tran, T. H. Thi, D. Nguyen","doi":"10.1177/08839115221098058","DOIUrl":"https://doi.org/10.1177/08839115221098058","url":null,"abstract":"Injectable hydrogels offer a wide range of attractive benefits in drug delivery applications, such as non-invasive administration, easy drug incorporation and locally controlled release at the target sites. Herein, we designed a simple and efficient method to prepare injectable hydrogels composed of gelatin and cyclodextrin (CD) for high loading capacity of hydrophobic drugs. The hydrogels were formed by thiol-functionalized gelatin (GSH) and βCD-vinyl sulfone (βCD-VS) as cross-linker, via thiol-ene “click” chemistry. Hydrogels comprising of different cross-linker feed amount were investigated in terms of their physico-chemical properties, such as gelation time, mechanical strength, swelling ratio, porosity and degradation rates. For the use as a drug delivery vehicle, dexamethasone (DEX), a commonly anti-inflammatory, immunosuppressive but poorly water soluble drug was chosen to show the high drug loading capacity and prolonged drug release of hydrogels. The drug release was found to be depended on the concentration of βCD-VS due to the drug-CD interaction. In vitro cytotoxicity experiment also showed the cell compatibility of these hydrogels against human dermal fibroblasts. In summary, we expect this gelatin-CD “click” hydrogel will be a promising candidate for localized and long-term delivery of hydrophobic drugs. Graphical Abstract","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90664018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-17DOI: 10.1177/08839115221098059
M. Moradi, Aboulfazl Barati, S. Moradi, M. Arjomandzadegan
Wound healing is a complicated process requiring appropriate environment to accelerate healing process. In the recent years, many wound dressings have been developed for treating various kinds of wounds. In this study, we aimed to develop a novel dressing with high ability of burn wound healing and minimum side effects. Carboxymethyl cellulose (CMC) based hydrogels containing Hypericum perforatum were developed by grafting methacrylic acid and acrylamide onto CMC to produce a good mechanical strength dressing. Covalent crosslinking, which is responsible for stable mechanical structure, led to a 3D structure with appropriate water vapor transmission rate (2950 g/m2/day), controlled drug release (33% in 78 h), and great burn healing ability (almost complete healing in 10 day). The hydrogel has proper antimicrobial activity against the tested microorganisms. Zone of inhibition against E.coli was the higher in comparison with S. aureus and Candida. Minimum inhibitory concentration (MIC) for C. albicans, S. aureus, and E. coli were as 6, 4, and 5 mg/ml of H. perforatum. In vivo experiments on rats revealed that wound healing process by loaded hydrogels was faster in comparison with control group. All the results indicated that prepared hydrogel has the capability to accelerate burn wound healing process.
伤口愈合是一个复杂的过程，需要适当的环境来加速愈合过程。近年来，为了治疗各种各样的伤口，开发了许多伤口敷料。在这项研究中，我们旨在开发一种具有高愈合能力和最小副作用的新型敷料。采用甲基丙烯酸和丙烯酰胺接枝法制备了以贯叶连翘为原料的羧甲基纤维素水凝胶。共价交联负责稳定的机械结构，形成了具有适当的水蒸气透过率(2950 g/m2/day)、药物释放可控(78 h 33%)、良好的烧伤愈合能力(10天几乎完全愈合)的三维结构。所述水凝胶对所测微生物具有适当的抗菌活性。对大肠杆菌的抑制区较金黄色葡萄球菌和念珠菌高。对白色念珠菌、金黄色葡萄球菌和大肠杆菌的最低抑菌浓度分别为6、4和5 mg/ml。大鼠体内实验表明，负载水凝胶的伤口愈合过程比对照组更快。结果表明，制备的水凝胶具有促进烧伤创面愈合的作用。
{"title":"CMC-based hydrogels loaded with Hypericum perforatum nanoemulsion for potential wound dressing applications","authors":"M. Moradi, Aboulfazl Barati, S. Moradi, M. Arjomandzadegan","doi":"10.1177/08839115221098059","DOIUrl":"https://doi.org/10.1177/08839115221098059","url":null,"abstract":"Wound healing is a complicated process requiring appropriate environment to accelerate healing process. In the recent years, many wound dressings have been developed for treating various kinds of wounds. In this study, we aimed to develop a novel dressing with high ability of burn wound healing and minimum side effects. Carboxymethyl cellulose (CMC) based hydrogels containing Hypericum perforatum were developed by grafting methacrylic acid and acrylamide onto CMC to produce a good mechanical strength dressing. Covalent crosslinking, which is responsible for stable mechanical structure, led to a 3D structure with appropriate water vapor transmission rate (2950 g/m2/day), controlled drug release (33% in 78 h), and great burn healing ability (almost complete healing in 10 day). The hydrogel has proper antimicrobial activity against the tested microorganisms. Zone of inhibition against E.coli was the higher in comparison with S. aureus and Candida. Minimum inhibitory concentration (MIC) for C. albicans, S. aureus, and E. coli were as 6, 4, and 5 mg/ml of H. perforatum. In vivo experiments on rats revealed that wound healing process by loaded hydrogels was faster in comparison with control group. All the results indicated that prepared hydrogel has the capability to accelerate burn wound healing process.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89249153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1177/08839115221095154
Daniel Kulakowski, Rasika M Phansalkar, Ariene A Leme-Kraus, James McAlpine, Shao-Nong Chen, Guido F Pauli, Sriram Ravindran, Ana K Bedran-Russo
Aim: Grape seed extract contains a complex mixture of proanthocyanidins (PACs), a plant biopolymer used as a biomaterial to improve reparative and preventive dental therapies. Co-polymerization of PACs with type I collagen mechanically reinforces the dentin extracellular matrix. This study assessed the biocompatibility of PACs from grape seed extract on dental pulp stem cells (DPSCs) in a model simulating leaching through dentin to the pulp cavity. The aim was to determine the type of PACs (galloylated vs. non-galloylated) within grape seed extract that are most compatible with dental pulp tissue.
Methodology: Human demineralized dentin was treated with selectively-enriched dimeric PACs prepared from grape seed extract using liquid-liquid chromatography. DPSCs were cultured within a 2D matrix and exposed to PAC-treated dentin extracellular matrix. Cell proliferation was measured using the MTS assay and expression of odontoblastic genes was analyzed by qRT-PCR. Categorization of PACs leaching from dentin was performed using HPLC-MS.
Results: Enriched dimeric fractions containing galloylated PACs increased the expression of certain odontoblastic genes in DPSCs, including Runt-related transcription factor 2 (RUNX2), vascular endothelial growth factor (VEGF), bone morphogenetic protein 2 (BMP2), basic fibroblast growth factor (FGF2), dentin sialophosphoprotein (DSPP) and collagen, type I, alpha 1 (COLI). Galloylated dimeric PACs also exhibited minor effects on DPSC proliferation, resulting in a decrease compared to control after five days of treatment. The non-galloylated dimer fraction had no effect on these genes or on DPSC proliferation.
Conclusions: Galloylated PACs are biocompatible with DPSCs and may exert a beneficial effect on cells within dental pulp tissue. The observed increase in odontoblastic genes induced by galloylated PACs together with a decrease in DPSC proliferation is suggestive of a shift toward cell differentiation. This data supports the use of dimeric PACs as a safe biomaterial, with galloylated dimeric PACs exhibiting potential benefits to odontoblasts supporting dentin regeneration.
{"title":"Galloylated proanthocyanidins in dentin matrix exhibit biocompatibility and induce differentiation in dental stem cells.","authors":"Daniel Kulakowski, Rasika M Phansalkar, Ariene A Leme-Kraus, James McAlpine, Shao-Nong Chen, Guido F Pauli, Sriram Ravindran, Ana K Bedran-Russo","doi":"10.1177/08839115221095154","DOIUrl":"https://doi.org/10.1177/08839115221095154","url":null,"abstract":"<p><strong>Aim: </strong>Grape seed extract contains a complex mixture of proanthocyanidins (PACs), a plant biopolymer used as a biomaterial to improve reparative and preventive dental therapies. Co-polymerization of PACs with type I collagen mechanically reinforces the dentin extracellular matrix. This study assessed the biocompatibility of PACs from grape seed extract on dental pulp stem cells (DPSCs) in a model simulating leaching through dentin to the pulp cavity. The aim was to determine the type of PACs (galloylated vs. non-galloylated) within grape seed extract that are most compatible with dental pulp tissue.</p><p><strong>Methodology: </strong>Human demineralized dentin was treated with selectively-enriched dimeric PACs prepared from grape seed extract using liquid-liquid chromatography. DPSCs were cultured within a 2D matrix and exposed to PAC-treated dentin extracellular matrix. Cell proliferation was measured using the MTS assay and expression of odontoblastic genes was analyzed by qRT-PCR. Categorization of PACs leaching from dentin was performed using HPLC-MS.</p><p><strong>Results: </strong>Enriched dimeric fractions containing galloylated PACs increased the expression of certain odontoblastic genes in DPSCs, including Runt-related transcription factor 2 (RUNX2), vascular endothelial growth factor (VEGF), bone morphogenetic protein 2 (BMP2), basic fibroblast growth factor (FGF2), dentin sialophosphoprotein (DSPP) and collagen, type I, alpha 1 (COLI). Galloylated dimeric PACs also exhibited minor effects on DPSC proliferation, resulting in a decrease compared to control after five days of treatment. The non-galloylated dimer fraction had no effect on these genes or on DPSC proliferation.</p><p><strong>Conclusions: </strong>Galloylated PACs are biocompatible with DPSCs and may exert a beneficial effect on cells within dental pulp tissue. The observed increase in odontoblastic genes induced by galloylated PACs together with a decrease in DPSC proliferation is suggestive of a shift toward cell differentiation. This data supports the use of dimeric PACs as a safe biomaterial, with galloylated dimeric PACs exhibiting potential benefits to odontoblasts supporting dentin regeneration.</p>","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353770/pdf/nihms-1857444.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9839823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1177/08839115221098056
Filiz Kara, E. Aksoy, S. Aksoy, N. Hasirci
Silver nanoparticles with potential antibacterial properties are included in biomaterials for the production of medical devices, which are used for diagnoses or treatment purposes. The aim of the current study was coating the polyurethane (PU) films with silver nanoparticles (AgNPs) due to their antibacterial efficacy. PU films were first modified by chitosan (CH), treated with AgNO3 to let CH chelate with silver ions, and then treated with vitamin-C (vit C) or glucose (Glu) to reduce the adsorbed ions to atomic silver to form AgNPs. The surfaces of the films were examined by ATR-FTIR, XPS, XRD, and SEM. Chemical bond formation between CH and Ag ions and AgNPs were determined by ATR-FTIR. Meanwhile, XPS and SEM analyses proved the presence of reduced metallic silver and nanoparticles on the film surfaces, respectively. According to the SEM analyses, a homogeneous distribution of AgNPs, with sizes 99–214 nm and 37–54 nm, on the film surfaces were obtained depending on Glu or vit C reduction, respectively. The films presented excellent antibacterial performance against Gram positive Staphylococcus epidermidis (S. epidermidis). These results suggested that the mentioned green technology can be easily applied to obtain AgNP coated polymeric surfaces with very high antibacterial efficacy. Although there are some studies dealing with AgNP formation on PU sponges or fibers, to the best of our knowledge, this is the first study showing AgNP formation on the CH conjugated PU films.
{"title":"Coating of silver nanoparticles on polyurethane film surface by green chemistry approach and investigation of antibacterial activity against S. epidermidis","authors":"Filiz Kara, E. Aksoy, S. Aksoy, N. Hasirci","doi":"10.1177/08839115221098056","DOIUrl":"https://doi.org/10.1177/08839115221098056","url":null,"abstract":"Silver nanoparticles with potential antibacterial properties are included in biomaterials for the production of medical devices, which are used for diagnoses or treatment purposes. The aim of the current study was coating the polyurethane (PU) films with silver nanoparticles (AgNPs) due to their antibacterial efficacy. PU films were first modified by chitosan (CH), treated with AgNO3 to let CH chelate with silver ions, and then treated with vitamin-C (vit C) or glucose (Glu) to reduce the adsorbed ions to atomic silver to form AgNPs. The surfaces of the films were examined by ATR-FTIR, XPS, XRD, and SEM. Chemical bond formation between CH and Ag ions and AgNPs were determined by ATR-FTIR. Meanwhile, XPS and SEM analyses proved the presence of reduced metallic silver and nanoparticles on the film surfaces, respectively. According to the SEM analyses, a homogeneous distribution of AgNPs, with sizes 99–214 nm and 37–54 nm, on the film surfaces were obtained depending on Glu or vit C reduction, respectively. The films presented excellent antibacterial performance against Gram positive Staphylococcus epidermidis (S. epidermidis). These results suggested that the mentioned green technology can be easily applied to obtain AgNP coated polymeric surfaces with very high antibacterial efficacy. Although there are some studies dealing with AgNP formation on PU sponges or fibers, to the best of our knowledge, this is the first study showing AgNP formation on the CH conjugated PU films.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84986669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1177/08839115221095152
Forough N Golafzani, A. Vaziri, M. Javanmardi, Fatemeh Seyfan, M. Yazdanifar, Sepideh Khaleghi
Objective: Nanoparticle-based drug delivery systems (DDSs) have been playing a considerable role in the eradication of cancer. In this experimental study, we designed and synthesized folic acid (FA)-decorated chitosan (CS) nanocarrier for targeted delivery of miR-126 (as a therapeutic agent) to lung cancer A549 cells. Materials and methods: Therefore, the FA-CS-miR-126 nano-complex was perfectly developed and characterized by various analytical devices such as Fourier transform infrared (FT-IR) and dynamic light scattering (DLS) spectroscopies and as well as transmission electron microscopy (TEM). The size was determined lower than 100 nm for synthetics. Then, a gel retardation assay was performed to investigate the entrapment efficiency of nano-complex. Afterward, the sort of in vitro assays was implemented on A549 (FA receptor-positive lung cancer cell line) and MRC5 (normal human diploid cell line) to evaluate the therapeutic efficiency of FA-CS-miR-126. Results: As the cell viability (40.7 ± 2.98% cell viability after 72 h treatment with 500 nM), migration assay (weaker migration after 24 h and 48 h), apoptotic and autophagy genes expression level (Caspse9: sixfolds; BAX: 17 folds; ATG5: fourfolds; and BECLIN1: threefolds more than the control group), the reduced expression level of EGF-L7, as a target gene for miR-126 was evaluated by Real-Time PCR too, then, cell cycle arrest (8.66% of cells in sub-G1 phase), and cell apoptosis assay (21.0% of cancer cell in late apoptosis phase) were scrutinized. Conclusion: These results are remarkably approved the biocompatible and efficient performance of FA-CS-miR-126 as a promising DDS. Graphical Abstract
{"title":"Delivery of miRNA-126 through folic acid-targeted biocompatible polymeric nanoparticles for effective lung cancer therapy","authors":"Forough N Golafzani, A. Vaziri, M. Javanmardi, Fatemeh Seyfan, M. Yazdanifar, Sepideh Khaleghi","doi":"10.1177/08839115221095152","DOIUrl":"https://doi.org/10.1177/08839115221095152","url":null,"abstract":"Objective: Nanoparticle-based drug delivery systems (DDSs) have been playing a considerable role in the eradication of cancer. In this experimental study, we designed and synthesized folic acid (FA)-decorated chitosan (CS) nanocarrier for targeted delivery of miR-126 (as a therapeutic agent) to lung cancer A549 cells. Materials and methods: Therefore, the FA-CS-miR-126 nano-complex was perfectly developed and characterized by various analytical devices such as Fourier transform infrared (FT-IR) and dynamic light scattering (DLS) spectroscopies and as well as transmission electron microscopy (TEM). The size was determined lower than 100 nm for synthetics. Then, a gel retardation assay was performed to investigate the entrapment efficiency of nano-complex. Afterward, the sort of in vitro assays was implemented on A549 (FA receptor-positive lung cancer cell line) and MRC5 (normal human diploid cell line) to evaluate the therapeutic efficiency of FA-CS-miR-126. Results: As the cell viability (40.7 ± 2.98% cell viability after 72 h treatment with 500 nM), migration assay (weaker migration after 24 h and 48 h), apoptotic and autophagy genes expression level (Caspse9: sixfolds; BAX: 17 folds; ATG5: fourfolds; and BECLIN1: threefolds more than the control group), the reduced expression level of EGF-L7, as a target gene for miR-126 was evaluated by Real-Time PCR too, then, cell cycle arrest (8.66% of cells in sub-G1 phase), and cell apoptosis assay (21.0% of cancer cell in late apoptosis phase) were scrutinized. Conclusion: These results are remarkably approved the biocompatible and efficient performance of FA-CS-miR-126 as a promising DDS. Graphical Abstract","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88594984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1177/08839115221095254
Linling Huang, Yi Jiang, Xueping Liu, Ying Guo, Yanfei Feng, Peng Pan, Mingzhong Li, Yu Liu
Vascularization is a key challenge in the regeneration of tissues containing blood vessels. In this study, spermine was used for cationic modification of Antheraea pernyi silk fibroin (ASF) to synthesize cationized ASF (CASF). CASF/Ad complexes prepared by coating adenovirus (Ad) with CASF were used as delivery vectors for vascular endothelial growth factor 165 and angiopoietin-1 dual genes. The results showed that the zeta potential of the Ad was reversed from −7.75 mV to approximately +8.40 mV after CASF coating, and the sizes of the CASF/Ad complexes were 200 to 290 nm. Furthermore, human umbilical vein endothelial cells HUVECs were cocultured and infected with CASF/Ad in vitro. The results of confocal laser scanning microscopy, flow cytometry and CCK-8 assay showed that coating Ad with CASF at concentration of 20 and 50 µg/mL not only reduced the cytotoxicity of naked Ad, but also significantly promoted cell proliferation. Therefore, the CASF/Ad complexes could be beneficial to reduce the dosage of Ad and the potential toxicity risk of high doses of Ad in vivo, which has the potential of application to promote vascular network regeneration.
{"title":"Antheraea pernyi silk fibroin-coated adenovirus as a VEGF165-Ang-1 dual gene delivery vector","authors":"Linling Huang, Yi Jiang, Xueping Liu, Ying Guo, Yanfei Feng, Peng Pan, Mingzhong Li, Yu Liu","doi":"10.1177/08839115221095254","DOIUrl":"https://doi.org/10.1177/08839115221095254","url":null,"abstract":"Vascularization is a key challenge in the regeneration of tissues containing blood vessels. In this study, spermine was used for cationic modification of Antheraea pernyi silk fibroin (ASF) to synthesize cationized ASF (CASF). CASF/Ad complexes prepared by coating adenovirus (Ad) with CASF were used as delivery vectors for vascular endothelial growth factor 165 and angiopoietin-1 dual genes. The results showed that the zeta potential of the Ad was reversed from −7.75 mV to approximately +8.40 mV after CASF coating, and the sizes of the CASF/Ad complexes were 200 to 290 nm. Furthermore, human umbilical vein endothelial cells HUVECs were cocultured and infected with CASF/Ad in vitro. The results of confocal laser scanning microscopy, flow cytometry and CCK-8 assay showed that coating Ad with CASF at concentration of 20 and 50 µg/mL not only reduced the cytotoxicity of naked Ad, but also significantly promoted cell proliferation. Therefore, the CASF/Ad complexes could be beneficial to reduce the dosage of Ad and the potential toxicity risk of high doses of Ad in vivo, which has the potential of application to promote vascular network regeneration.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82064438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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