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Evaluation of the Antibacterial Potential of the Phytochemicals in Leaf Extracts of Newboudia laevis on Uropathogens 新妇叶提取物中植物化学物质对尿路病原菌抑菌潜力的评价
Pub Date : 2021-01-01 DOI: 10.35248/2155-9597.21.S10.003
F. Alao, Z. S. Ololade, Yusuf A. Fagge
This study was conducted to determine the secondary metabolites and antibacterial potential of the leaf extracts Newbouldia laevis on some uropathogen strains and also to carry out phytochemical screening of the sample. The antibacterial activity of N. laevis leaves extracted with methanol, hot and cold water were evaluated against medically important bacteria strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus saprophyticus and Enterococcus faecalis. The results showed a remarkable inhibition of the bacteria growth against the tested organisms with Staphylococcus saprophyticus had highest activity (20 mm). In general, all the tested organisms showed a significant activity with the extracts. The conventional antibiotics used against these organisms showed that all the isolates were resistant to augmentin, ceftazidime, cefuroxime, and cloxacillin. The phytochemical analysis of the plants was carried out. The microbial activity of the Newbouldia laevis was due to the presence of various secondary metabolites. The GC-MS analysis showed that the most abundant phytochemical in the extract is linoleoyl chloride (76.3%). Hence, these plants can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals research activities.
本研究旨在测定青叶提取物对部分尿路病原菌的次生代谢物和抑菌潜力,并对样品进行植物化学筛选。以甲醇提取液、热水提取液和冷水提取液分别对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、腐生葡萄球菌和粪肠球菌等重要细菌进行抑菌活性评价。结果表明,对腐生葡萄球菌生长有明显的抑制作用,其中腐生葡萄球菌活性最高(20 mm)。总的来说,所有被测生物对提取物都表现出显著的活性。对这些细菌使用的常规抗生素表明,所有分离株都对augmentin、头孢他啶、头孢呋辛和cloxacillin耐药。对这些植物进行了植物化学分析。纽博尔迪亚湖的微生物活性是由于各种次生代谢物的存在。GC-MS分析表明,该提取物中含量最高的植物化学物质为亚油酰氯(76.3%)。因此,这些植物可以用来发现具有生物活性的天然产物,这些产物可以作为开发新药物研究活动的先导。
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引用次数: 0
Assessment of Knowledge and Practice of House Hold Water Treatment and Associated Factors in Rural Kebeles of Degadamot Woreda, North-West Ethiopia, 2020 2020年埃塞俄比亚西北部Degadamot Woreda农村Kebeles家庭用水处理知识和实践及相关因素评估
Pub Date : 2021-01-01 DOI: 10.35248/2155-9597.21.12.403
D. Tsegaye, Yizengaw Aniley, Belete Negese, Zemen Mengesha
Background: Household water treatment and safe storage interventions are proven to improve water quality and reduce diarrheal disease incidence in developing countries. Almost 90% of the rural population of Ethiopia did not practice any alternative water treatment methods which would pose them to high public health risks. The aim of this study was therefore to identify factors of house hold water treatment practice and knowledge in rural kebeles of Degadamot woreda, North West Ethiopia, 2020. Methods: A Community-based cross-sectional study design was conducted in Degadamot woreda, North West Ethiopia, 2020. A Multistage sampling technique was carried out to select 845 sample households. Data were entered into the Epi-Data version 4.1 and analyzed using SPSS Version 20. Binary Logistic regression analysis was conducted to identify factors of house hold water treatment practice and knowledge. Results: In this study, 14% of participants were practicing household water treatment and 28.2% had knowledge on household water treatment practice. Educational status, income earning >600 ETB per month, number of under five children in the household, ways to fetch water and knowledge on HWT were factors significantly associated with household water treatment practice. And educational status, marital status, source of water for drinking, number of containers to store water two, three and above: and place of handling utensils were factors which had significant association with knowledge of house hold water treatment practice. Conclusion: Factors that were significantly associated with household water treatment practice were educational status, income, number of under-five children, ways to fetch drinking water and good knowledge about household water treatment.
背景:在发展中国家,家庭水处理和安全储存干预措施已被证明可以改善水质并减少腹泻病的发病率。埃塞俄比亚几乎90%的农村人口没有采用任何会给他们带来高度公共健康风险的替代水处理方法。因此,本研究的目的是确定2020年埃塞俄比亚西北部Degadamot woreda农村kebeles家庭用水处理实践和知识的影响因素。方法:以社区为基础的横断面研究设计于2020年在埃塞俄比亚西北部的Degadamot woreda进行。采用多阶段抽样方法,抽取845户样本家庭。数据输入Epi-Data 4.1版本,使用SPSS version 20进行分析。采用二元Logistic回归分析,确定家庭用水处理实践和知识的影响因素。结果:在本研究中,14%的参与者从事家庭水处理实践,28.2%的参与者了解家庭水处理实践。受教育程度、每月收入>600 ETB、家庭中五岁以下儿童的数量、取水方式和HWT知识是与家庭水处理实践显著相关的因素。教育程度、婚姻状况、饮用水来源、储存水的容器数量(2、3及以上)和处理器具的位置是与家庭用水处理实践知识显著相关的因素。结论:影响家庭水处理实践的因素有教育程度、收入、5岁以下儿童人数、取水方式和家庭水处理知识。
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引用次数: 5
Bacterial Blood Stream Infection in Southern Ethiopia 埃塞俄比亚南部的细菌性血流感染
Pub Date : 2021-01-01 DOI: 10.35248/2155-9597.21.S8.003
M. Hailemariam, Tsegaye Alemayehu, asnakech agegnehu, Mulubrhan Ali, Enkosilassie Mitiku, Elshaday Azerefegne
Background: Children’s blood stream infections mainly due to resistant pathogens found to be a higher risk of hospital mortality in low and middle income countries. This study intended to assess the bacterial profile and drug resistance of isolates of blood stream infection among under- 5 children in Southern Ethiopia. Methods: Retrospectively lab recorded data’s were retrieved to assess the bacterial isolates and rates antibiotic resistance at Hawassa University Comprehensive Specialized Hospital. Results: Of 323 bloods culture performed 116 (35.9%) were positive for potential bacterial BSI. Positivity of blood culture decrease with age, hence neonates blood stream infection stream infection were higher than other age groups (OR, 3; 95% CI 1.5–5.1; p=0.001). The major gram positive isolate CoNS and S. aureus have shown highest level of resistance to penicillin 61.3% and tetracycline 78.8% whereas less level of resistance was reported to Meropenem 6.4%, Ceftriaxone 13% and doxycycline 13%. Although most of gram negatives isolates were resistant for tested antibiotics, K. pneumoniae has shown leaser resistance to cefotaxime and chloramphenicol. Conclusion: A high level of blood stream infection accompanied by high drug resistant isolates was reported. The great burden in neonate than other children was reported. As a result to reduce the burden the hospital administrators need to facilities effective infection prevention and control programmes, effective hygiene practices and well-functioning environment along with educating patient about proper hygiene practices.
背景:在低收入和中等收入国家,主要由耐药病原体引起的儿童血流感染是医院死亡率较高的风险之一。本研究旨在评估埃塞俄比亚南部5岁以下儿童血液感染分离株的细菌谱和耐药性。方法:回顾性检索哈瓦萨大学综合专科医院的实验室记录资料,评估细菌分离株及耐药性。结果:323例血培养中,潜在细菌BSI阳性116例(35.9%)。血培养阳性率随年龄增长而降低,因此新生儿血流感染率高于其他年龄组(OR, 3;95% ci 1.5-5.1;p = 0.001)。主要革兰氏阳性分离物con和金黄色葡萄球菌对青霉素和四环素的耐药性最高,分别为61.3%和78.8%,而对美罗培南、头孢曲松和强力霉素的耐药性较低,分别为6.4%、13%和13%。虽然大多数革兰氏阴性分离株对所测抗生素具有耐药性,但肺炎克雷伯菌对头孢噻肟和氯霉素的耐药性较弱。结论:报告了高水平的血流感染并伴有高耐药分离株。据报道,新生儿的负担比其他儿童重。因此,为了减轻负担,医院管理人员需要建立有效的感染预防和控制规划、有效的卫生习惯和运转良好的环境,并对患者进行适当的卫生习惯教育。
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引用次数: 0
Oxysterols: A New Hope for an Effective and Safe Therapy for COVID-19, Another Light at the End of the Tunnel 氧甾醇:有效安全治疗COVID-19的新希望,隧道尽头的另一束光
Pub Date : 2021-01-01 DOI: 10.35248/2155-9597.21.12.404
F. Parhami, Feng Wang, F. Stappenbeck
The COVID-19 pandemic and its lasting consequences have dealt a huge, irreversible blow to the vitality of the global economy and the livelihood of all populations affected by it. Millions of lives lost, businesses destroyed, hopes and dreams shattered and the pandemic still persists despite the remarkable impact of newly developed vaccines. Unfortunately, there are still many uncertainties that threaten our future: • Harmful new variants of SARS-CoV-2, the COVID-19 virus, emerging from a constantly mutating virus that may render the existing vaccines less effective or even ineffective. • The colossal task of vaccinating the entire world population, combined with a likely need for repeat vaccination. • The side effects of the available vaccines, especially in certain segments of the population, including young men and pregnant or to be pregnant women. Unfortunately, not much is currently known about potential long term side effects of the newly developed vaccines.
2019冠状病毒病大流行及其长期后果给全球经济活力和所有受影响人群的生计造成了巨大而不可逆转的打击。数百万人失去了生命,企业被摧毁,希望和梦想破灭,尽管新开发的疫苗产生了显著影响,但这一流行病仍然存在。不幸的是,仍有许多不确定因素威胁着我们的未来:•从不断变异的病毒中出现的有害的新变体SARS-CoV-2,即COVID-19病毒,可能会降低现有疫苗的有效性,甚至无效。•为全世界人口接种疫苗的艰巨任务,加上可能需要重复接种。•现有疫苗的副作用,特别是对某些人群,包括青年男子和孕妇或孕妇的副作用。不幸的是,目前对新开发疫苗的潜在长期副作用知之甚少。
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引用次数: 0
The SARS-Cov-2 Novel Coronavirus: A Reflection about Enhancement of High-Resolution Microscopic Images 新型冠状病毒SARS-Cov-2:对高分辨率显微图像增强的思考
Pub Date : 2021-01-01 DOI: 10.35248/2155-9597.21.S10.004
R. Rodríguez, Mondeja Ba, Lau Ld, A. Vizcaino, Acosta Ef, Y. González
After a year of hard battling with the novel coronavirus SARS Cov-2, the COVID-19 pandemic continues had a catastrophic effect on society and health worldwide. This pandemic has changed labor and economic relations in almost every country in the world, and the investment that has been made in the development of new treatment protocols and the creation of vaccines has been enormous. Important laboratories, hospitals and research centers around the world have been fighting against SARS-Cov-2, and within these researches computer vision has played a prominent role. The main aim of this work is to carry out a reflection on the enhancement of the microscopic images of the novel coronavirus SARS-Cov-2 from the results obtained and published. We will analyze the effectiveness of the algorithms proposed to highlight the S-spikes, and we will detail why deep learning, despite the popularity achieved, in this case was not beneficial.
在与新型冠状病毒SARS - Cov-2进行了一年的艰苦斗争之后,COVID-19大流行继续对全球社会和健康造成灾难性影响。这场大流行几乎改变了世界上每个国家的劳动和经济关系,在制定新的治疗方案和研制疫苗方面进行了巨大的投资。世界各地的重要实验室、医院和研究中心都在与SARS-Cov-2作斗争,计算机视觉在这些研究中发挥了突出作用。本工作的主要目的是根据已获得和发表的结果,对新型冠状病毒SARS-Cov-2的显微图像增强进行反思。我们将分析提出的算法的有效性,以突出s -spike,我们将详细说明为什么深度学习,尽管取得了普及,在这种情况下是无益的。
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引用次数: 1
Strongyloides Stercoralis Infection in a Case of Ankylosing Spondylitis 强直性脊柱炎1例粪类圆线虫感染
Pub Date : 2021-01-01 DOI: 10.35248/2155-9597.21.12.393
L. Pezone
A 69 year old man was admitted in the hospital with chief complaints of fever of 2 days duration, swelling and pain of lower limb and knee joint for 4 days. He was a known case of ankylosing spondylitis for last 10 years with chronic intake of corticosteroids. On admission the patient was diagnosed with right lower limb cellulitis and right lung consolidation and pleural effusion. Blood culture and synovial fluid culture showed growth of Staphylococcus aureus. 2 days later patient complained of diarrhoea. Routine stool examination showed plenty of rhabditiform Strongyloides Stercoralis larva. The patient was treated with Ivermectin for Strongyloides and was discharged after total resolution of infection with advice of regular follow-ups for any dissemination or hyperinfection.
男69岁,主诉发热2天,下肢及膝关节肿胀疼痛4天。他是一个已知的强直性脊柱炎病例,近10年慢性摄入皮质类固醇。入院时诊断为右下肢蜂窝组织炎、右肺实变及胸腔积液。血培养和滑液培养均可见金黄色葡萄球菌生长。2天后,患者主诉腹泻。常规大便检查显示大量横纹肌状粪圆虫幼虫。患者用伊维菌素治疗圆形杆菌,感染完全解决后出院,并建议定期随访任何传播或过度感染。
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引用次数: 0
Severe Rhinocerebral Mucormycosis Case Developed after COVID-19 新型冠状病毒感染后发生的重症颅脑毛霉菌病1例
Pub Date : 2021-01-01 DOI: 10.36648/2155-9597.21.12.386
F. Sargın, Mert Akbulut, S. Karaduman, H. Sungurtekin
In this article, a case of rhinocerebral mucormycosis developing after COVID-19 infection in a diabetic patient is presented. Early diagnosis and treatment of those high morbidity conditions is vital to better outcomes.
本文报告1例糖尿病患者感染COVID-19后并发鼻-脑毛霉菌病。这些高发病率疾病的早期诊断和治疗对于改善预后至关重要。
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引用次数: 29
Sexually Transmitted Pathogens in Key Populations Attending the Institute of Social Hygiene Hospital in Dakar, Senegal 塞内加尔达喀尔社会卫生医院研究所关键人群中的性传播病原体
Pub Date : 2021-01-01 DOI: 10.35248/2155-9597.21.12.395
H. Diop-Ndiaye, A. Dieng, A. Gaye, A. Ba-Diallo, Lobach Sm, A. Tinè, M. Mboup, Ndiaye Ajs, Diaz Cf, B. Dembele, Ngom Cs, N. Sene, A. Diouf, A. Sow, A. Sarr, B. Ndiaye, H. Diagne, M. Camara, Boye Csb
Introduction: Chlamydia trachomatis urogenital infection is one of the leading causes of bacterial Sexually Transmitted Infections (STIs) and responsible for many complications. This study aimed to assess the prevalence of sexually transmitted pathogens in key populations in Senegal. Materials and methods: A retrospective study from January to December 2018 was carried out at the Institute of Social Hygiene in 2 key populations namely Men who have Sex with Men (MSM) and Female Sex Workers (FSW) presented symptoms of STIs. For each patient, blood samples and urethral or vaginal sample were collected. Diagnostic of STIs microorganisms including Treponema pallidum, C. trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis was performed. Results: Two hundred fourteen patients (173 FSW and 41 MSMS) with STIs symptoms were included in this study. STI diagnosis was confirmed in 176 participants giving an overall STIs rate of 82% (176/214). Among them, 80% (141/176) were FSW and 20% (35/176) were MSM. C. trachomatis was found in 55% of cases (97/176) followed by N. gonorrhoeae (18%; n=32/176), T. vaginalis (15%; n=26/176) and T. Pallidum (12%; n=21/176). Interestingly, C. trachomatis infection was exclusively detected in FSW with a rate of 68.8% (97/141). In addition, C. trachomatis was associated with other STIs agents in 22 cases (23%) namely T. pallidum (5.2%; n=5/97), N. gonorrhoeae (3.1%; n=3/97), and T. vaginalis (14.4%; n=14/97). C. trachomatis was found in all age groups, however, young people (<30 years) seems to be more affected with 58.8% (57/97). Conclusion: This study showed a predominance of C. trachomatis infections among FSW suggesting the importance to consider this STI’s pathogen in the management of key populations in Senegal.
简介:泌尿生殖道沙眼衣原体感染是细菌性传播感染(STIs)的主要原因之一,并导致许多并发症。本研究旨在评估塞内加尔关键人群中性传播病原体的流行情况。材料与方法:2018年1 - 12月在社会卫生研究所对出现性传播感染症状的男男性行为者(MSM)和女性性工作者(FSW) 2个重点人群进行回顾性研究。对每位患者采集血液样本和尿道或阴道样本。对梅毒螺旋体、沙眼衣原体、淋病奈瑟菌、阴道毛滴虫等性传播感染微生物进行诊断。结果:本研究纳入了214例性传播感染症状患者(173例FSW和41例MSMS)。在176名参与者中确诊了性传播感染,总体性传播感染率为82%(176/214)。其中FSW占80% (141/176),MSM占20%(35/176)。沙眼衣原体占55%(97/176),其次是淋病奈瑟菌(18%;n=32/176),阴道绦虫(15%;n=26/176)和苍白球绦虫(12%;n = 21/176)。有趣的是,沙眼原体感染仅在FSW中检出,检出率为68.8%(97/141)。此外,22例(23%)沙眼衣原体与其他性传播感染相关,即苍白衣原体(5.2%);n=5/97),淋病奈瑟菌(3.1%;n=3/97),阴道绦虫(14.4%;n = 14/97)。沙眼原体在所有年龄组均有发现,但年轻人(<30岁)的感染率似乎更高,为58.8%(57/97)。结论:本研究显示沙眼原体感染在FSW中占主导地位,提示在塞内加尔重点人群的管理中考虑这种性传播感染病原体的重要性。
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引用次数: 0
A Commentary on the Corona Virus before the Next Pandemic 在下一次大流行之前对冠状病毒的评论
Pub Date : 2021-01-01 DOI: 10.35248/2155-9597.21.12.390
J. Slingenbergh
Making it to the next host goes to the heart of virus fitness. There are in this regard two broadly opposing virus life history strategies. Acute viruses tend to be replicative and swiftly transmit to the next host. Instead, a persistent virus may be shed in minor quantities, if at all, keeping virus replication costs and host damage to a minimum.
让它到达下一个宿主是病毒适应性的核心。在这方面有两种截然相反的病毒生活史策略。急性病毒倾向于复制,并迅速传播给下一个宿主。相反,如果有的话,持久性病毒可能会少量脱落,从而将病毒复制成本和对宿主的损害降至最低。
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引用次数: 0
Telemedicine in Time of COVID-19 COVID-19时代的远程医疗
Pub Date : 2021-01-01 DOI: 10.36648/2155-9597.21.12.389
John R Maese, D. Seminara, Zeel Shah
It is safe to say that 2020 has been an extraordinary year. In the past nine months, we have seen changes in telehealth that we could not accomplish in the past 15 years. The alignment of technology, policy and reimbursement have been powerful forces that have propelled telemedicine into the 21st century.
可以肯定地说,2020年是不平凡的一年。在过去9个月里,我们看到了远程医疗方面的变化,这是我们在过去15年里无法实现的。技术、政策和报销的结合是推动远程医疗进入21世纪的强大力量。
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引用次数: 1
期刊
Journal of Bacteriology & Parasitology
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