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Investigation of In Vivo Effects of Trichomonas Vaginalis on Visceral Organs 阴道毛滴虫体内对脏器影响的研究
Pub Date : 2017-11-13 DOI: 10.4172/2155-9597.1000327
Akyshbayeva Kulbarshin, S. Elmira, Ramazanova Bakyt, Kushugulova Almagul, Khassenbekova Zhanagul, S. Sergazy, M. Alía
Background: Urogenital trichomoniasis (UGT) is the most common sexually transmitted disease in Kazakhstan. Association of T. vaginalis infection with the development of ascites and multiple visceral abscesses was shown in experiments with intraperitoneal injection of infectious material. The effect of T. vaginalis on visceral organs in natural infection is unknown. Materials and methods: T.vaginalis effect on visceral organs was assessed with the model previously developed by us ("The Method of Urogenital Trichomoniasis Simulation", patent application No. 06 331 dated April 1, 2016). It was ethically carried out on animals with the approval of the KazNMU Ethics Committee (Registration No. 191). The material for histological and cytological studies was prepared in accordance with the conventional methods. Result: The changes was found in all visceral organs, most pronounced in hepatic parenchyma: parenchymal degeneration and necrosis of hepatocytes, significant edema with cellular infiltration in the portal tracts and separation of blood plasma from the formed elements in blood vessels representing changes in rheological properties of blood. Expressed changes were also observed in renal tissues. Less pronounced changes were observed in lung tissues. Conclusions: The results of our study showed high activity of T. vaginalis in visceral organs.
背景:泌尿生殖道毛滴虫病是哈萨克斯坦最常见的性传播疾病。在腹腔注射感染性物质的实验中,阴道绦虫感染与腹水和多发内脏脓肿的发生有关。自然感染时阴道梭菌对内脏器官的影响尚不清楚。材料和方法:阴道滴虫对内脏器官的影响采用我们先前开发的模型进行评估(“泌尿生殖器滴虫病模拟方法”,专利申请号06331,2016年4月1日)。该实验在动物身上进行,并得到了KazNMU伦理委员会(注册号191)的批准。按照常规方法制备组织学和细胞学研究材料。结果:所有脏器均有改变,以肝实质最明显:肝实质变性和肝细胞坏死,门管区明显水肿伴细胞浸润,血浆与血管内形成元素分离,血液流变学特性发生改变。在肾组织中也观察到表达变化。肺组织的变化不太明显。结论:本研究结果显示阴道梭菌在内脏器官中具有较高的活性。
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引用次数: 0
Genotypic Characteristics of Outpatient Antibiotic Resistance and Nosocomial Staphylococcus aureus Strains 门诊金黄色葡萄球菌耐药及院内金黄色葡萄球菌的基因型特征
Pub Date : 2017-11-13 DOI: 10.4172/2155-9597.1000328
Y. Tyurin, L. Bayazitova, T. A. Chazova, I. Reshetnikova, Tyurina Ny
The article presents the results of a study of antibiotic resistance of 191 methicillin-sensitive (MSSA) and methicillin-resistant (MRSA) strains Staphylococcus aureus (S. aureus), isolated from outpatient and hospital patients. The data of molecular-genetic typing of strains of S. aureus and established types of SCCmec cassettes. There were significant differences in the sample genotype MRSA strains isolated from patients and hospital outpatient profile. Isolates from outpatients, contained in its composition genetic elements SCCmec IV a, c, d types. MRSA, isolates from hospital patients profile were identified only type II SCCmec cassettes. Vancomycin, fusidic acid, mupirocin antistaphylococcal possessed high activity against MRSA and MSSA. MRSA were more resistant to antimicrobial drugs compared to MSSA. Azithromycin and clindamycin demonstrated moderate anti-staphylococcal activity. Revealed low activity of chloramphenicol, tetracycline and erythromycin against staphylococci strains.
本文报道了从门诊和住院患者中分离的191株甲氧西林敏感(MSSA)和耐甲氧西林(MRSA)金黄色葡萄球菌(S. aureus)的耐药性研究结果。金黄色葡萄球菌菌株的分子遗传分型数据和已建立的SCCmec磁带类型。从患者和医院门诊分离的MRSA菌株的基因型有显著差异。分离自门诊患者,其组成中含有SCCmec IV、a、c、d型遗传因子。从医院患者档案中分离出的MRSA仅被鉴定为II型SCCmec磁带。万古霉素、夫西地酸、莫匹罗星抗葡萄球菌对MRSA和MSSA均有较高的活性。与MSSA相比,MRSA对抗菌药物的耐药性更强。阿奇霉素和克林霉素表现出中等的抗葡萄球菌活性。发现氯霉素、四环素和红霉素对葡萄球菌的活性较低。
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引用次数: 0
RNA Interference and Nonspecific Controls in Parasitic Helminths 寄生蠕虫的RNA干扰和非特异性对照
Pub Date : 2017-11-03 DOI: 10.4172/2155-9597.1000325
M. M. Mourão, Sandra Grossi Gava
Despite of the remarkable success of RNA interference (RNAi) application in the free-living nematode Caenorhabditis elegans, the exploitation of this powerful technique to helminths parasites with complex life-cycle has been a challenge for parasitologists. It has proved to be effective only for certain parasite species and specific target genes. To date, RNAi is the only methodology available for reverse genetics in trematodes and combined to rescue studies (such heterologous complementation) have been the only alternative to genetic manipulation in nematodes and helminths parasites, thus this subject is of great interest to the scientific community involved in the field. The RNAi technique is widely used to assess gene function in helminths parasites, in order to elucidate their role in parasite development, mechanisms of drug resistance, and validate therapeutic targets for disease control. After fifteen years of the first report of RNAi in parasitic helminths, many advances have been achieved, but pitfalls remain as challenges in gene expression manipulation in these organisms. In addition to the methodological particularities of the RNAi technique for each group of helminths, there are still other reasons behind the slow progress of RNAi in those parasites, such; the lack of homology between genes related to parasitism and genes of model organisms and the complex life cycle of these organisms, which results in difficulties for in vitro cultivation. At this point, a wide assortment of approaches for doubled stranded RNA “delivery” has been proposed. Thus, deeper studies on fundamental aspects of the RNAi methodology in parasitic helminths, such as off-target and the use of controls, can be useful in determining the reason of variations between and within species, facilitating the experimental design and the use of RNAi in the study and eradication of helminths parasites.
尽管RNA干扰(RNA interference, RNAi)技术在秀丽隐杆线虫(Caenorhabditis elegans)上的应用取得了显著的成功,但将这种强大的技术应用于具有复杂生命周期的蠕虫寄生虫上一直是寄生虫学家面临的挑战。它已被证明仅对某些寄生虫种类和特定的靶基因有效。迄今为止,RNAi是唯一可用于线虫反向遗传学研究的方法,并且结合挽救研究(如异源互补)已成为线虫和蠕虫寄生虫遗传操作的唯一替代方法,因此该主题对参与该领域的科学界非常感兴趣。RNAi技术被广泛用于评估寄生虫基因功能,以阐明其在寄生虫发育中的作用、耐药机制,并验证疾病控制的治疗靶点。在首次报道RNAi在寄生蠕虫中的应用15年后,已经取得了许多进展,但是在这些生物体的基因表达操作方面仍然存在缺陷和挑战。除了每组蠕虫的RNAi技术在方法上的特殊性之外,RNAi技术在这些寄生虫中的缓慢进展还有其他原因,例如;寄生相关基因与模式生物基因缺乏同源性,且模式生物的生命周期复杂,给体外培养带来困难。在这一点上,已经提出了各种各样的双链RNA“递送”方法。因此,深入研究寄生蠕虫RNAi方法的基本方面,如脱靶和对照的使用,可以有助于确定物种之间和物种内部差异的原因,促进实验设计和RNAi在研究和根除寄生虫中的使用。
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引用次数: 0
Screening for Latent Tuberculosis Infection among Students of Healthcare Professions and Postgraduates of the Faculty of Medicine of the University of Palermo 巴勒莫大学医学院保健专业学生和研究生潜伏性结核感染筛查
Pub Date : 2017-10-26 DOI: 10.4172/2155-9597.1000324
M. G. Verso, D. Picciotto, N. LoCascio, E. NotoLaddeca, E. Amodio
Introduction and objective: Italy is a country with a low incidence of tuberculosis and in the last fifty years the annual number of TB cases decreased from 12,247 to 4,418, showing a reduction of approximately 64% in the number of cases and 71% in incidence. Despite of this encouraging trend, in the last years the epidemiology of tuberculosis changed and today it is a re-emerging infectious. The aim of this study is to measure the prevalence of positivity to tuberculosis infection (latent TB) in students, without any obvious manifestation of disease, attending degree courses of the health care professions and postgraduate medical courses of the School of Medicine of the University of Palermo, Italy. Materials and methods: A cross-sectional observational study in students of nursing, midwifery, dentistry degree courses and in resident physicians of postgraduate medical schools was carried out from January 2012 to July 2016. Mantoux test was performed and all positive cases were tested with Interferon-Gamma Release Assay (IGRA). Results: The 1,351 subjects evaluated, 25 (1.8%) resulted positive to Mantoux test; in 17 students (1.2%) the diagnosis was confirmed with IGRA. Positive cases were significantly more frequent among students attending Postgraduated Medical School Courses (p<0.001) and were older than negative cases (p<0.001). Conclusion: This study suggests that in our geographic area, latent TB shows a relatively low prevalence among students of medical schools. Despite of this evidence, and considering that several students have been found to be positive for TB, this infectious disease has to be considered a re-emerging biohazard that requires preventive strategies for the containment of the risk in exposed workers as well as in the general population.
前言和目标:意大利是一个结核病发病率低的国家,在过去五十年中,每年结核病病例数从12,247例减少到4,418例,表明病例数减少了约64%,发病率减少了71%。尽管有这种令人鼓舞的趋势,但在过去几年中,结核病的流行病学发生了变化,今天它是一种重新出现的传染病。本研究的目的是测量意大利巴勒莫大学医学院卫生专业学位课程和研究生医学课程中无明显疾病表现的学生结核感染(潜伏性结核)阳性的患病率。材料与方法:于2012年1月至2016年7月对护理学、助产学、牙科学位课程学生和医学研究生院住院医师进行横断面观察研究。所有阳性病例采用干扰素- γ释放试验(IGRA)检测。结果:1351例受试者中,25例(1.8%)为Mantoux试验阳性;17名学生(1.2%)经IGRA确诊。阳性病例在参加医学研究生课程的学生中明显多于阴性病例(p<0.001),且年龄大于阴性病例(p<0.001)。结论:本研究提示,在我国地理区域内,医学院学生的潜伏性结核病患病率相对较低。尽管有这些证据,并考虑到有几名学生已被发现对结核病呈阳性反应,这种传染病必须被视为一种重新出现的生物危害,需要采取预防战略,以遏制接触工人和一般人群的风险。
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引用次数: 0
Effectiveness of quick sequential organ failure assessment (qSOFA) as sepsis screening tool in the emergency department (ED) 快速顺序器官衰竭评估(qSOFA)作为急诊科脓毒症筛查工具的有效性
Pub Date : 2017-10-23 DOI: 10.4172/2155-9597-c1-044
Ajibola K Ibironke
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引用次数: 0
Tips for a successful technology transfer in life sciences 生命科学技术成功转让的秘诀
Pub Date : 2017-10-17 DOI: 10.4172/2155-9597-C1-040
A. Richard
We postulate a pathway to Alzheimer’s disease that begins with microbial pathogens, spirochetes, that make biofilms which upregulate the innate immune system. This leads to the destruction of the tissue and the formation of Aβ by known biochemical and microbiological pathways. The spirochetes also form biofilms intracellularly; and, in the process, create Aβ which stimulates the hyperphosphorylation of tau protein. This ultimately leads to the formation of neurofibrillary tangles and dendritic disintegration. All the steps in this pathway have been shown to be present by direct pathological observation or by known microbiological/biochemical pathways. As one proof of concept, things that negatively impact AD, such as diabetes, smoking, and certain drug exposures have been shown to influence one or another component of the pathway.
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引用次数: 1
Structure of AcrH-AopB chaperone-translocator complex reveals a role for membrane hairpins in type III secretion system translocon assembly AcrH-AopB伴侣-转运子复合物的结构揭示了膜发夹在III型分泌系统转运体组装中的作用
Pub Date : 2017-10-17 DOI: 10.4172/2155-9597-C1-036
Mok Yu-Keung
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引用次数: 23
Nanoparticle-based vaccine platforms against apicomplexan 基于纳米颗粒的抗顶复蛋白疫苗平台
Pub Date : 2017-10-03 DOI: 10.4172/2155-9597-C1-034
F. Debierre-Grockiego
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引用次数: 1
Linezolid use in Medicine Therapy against Multiresistant Bacteria-AReview 利奈唑胺在多重耐药细菌药物治疗中的应用综述
Pub Date : 2017-09-22 DOI: 10.4172/2155-9597.1000320
N. S. Mendes, M. Costa, T. Paulino, F. Agostinho, M. Ribeiro, R. Paludo, W. Rodrigues, C. B. Miguel
After the description of an element with ability to combat the infectious processes originating from bacteria, starts a race for survival between the interrelationship of species, bacterial and human. With the evolution scientifictechnical, the man was able to synthesize new antibacterial substances, on the other hand the mechanisms of gene evolution enabled the emergence of multidrug-resistant bacteria. Some of this organisms are frequent on hospital environment and have high adaptability to new drugs, such as Staphylococcus aureus and Enterococcus spp. resistant to oxacillin and vancomycin, considered drugs of choice against multidrug-resistant microorganisms. So, a new antibiotics class was developed, superior to vancomycin and oxazolidinone, the linezolid. Thus, the present study aimed at understanding the use of linezolid in drug therapy against multi-resistant bacteria. To perform this study, a literature review of last 10 years was performed. In 2002, after the liberation of the use of linezolid as treatment for infectious processes against gram-positive bacteria, this drug was commonly used throughout the world. Similarly, the pressure of natural selection stood out, and there were records of resistant strains to linezolid. As prospects for control of infections caused by these resistant strains, was approved by the FDA in 2014 the use of drugs with linezolid resistant anti-strains activity. However, we conclude that, in addition to natural selection and genetic variation process, human behavior regarding the use of antibiotics, increases the selection of resistant microorganisms to antibiotic, including linezolid.
在描述了一种能够对抗细菌感染过程的元素之后,开始了一场物种间的生存竞赛,细菌和人类。随着科学技术的进化,人类能够合成新的抗菌物质,另一方面,基因进化的机制使多重耐药细菌的出现成为可能。其中一些微生物常见于医院环境,对新药具有较高的适应性,如耐氧西林和万古霉素的金黄色葡萄球菌和肠球菌,被认为是对抗多重耐药微生物的首选药物。因此,开发了一种优于万古霉素和恶唑烷酮的新型抗生素——利奈唑胺。因此,本研究旨在了解利奈唑胺在多重耐药细菌药物治疗中的应用。为了进行这项研究,我们进行了近10年的文献回顾。2002年,利奈唑胺作为治疗革兰氏阳性菌感染过程的药物得到解放后,这种药物在世界各地得到普遍使用。同样,自然选择的压力也很突出,有对利奈唑胺产生抗性的菌株的记录。由于具有控制这些耐药菌株引起的感染的前景,FDA于2014年批准使用具有利奈唑胺耐药抗菌株活性的药物。然而,我们得出结论,除了自然选择和遗传变异过程外,人类使用抗生素的行为增加了抗生素耐药微生物的选择,包括利奈唑胺。
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引用次数: 0
Antibiotic Resistance Bacteria from Rivers Water in Algeria 阿尔及利亚河水中的抗生素耐药性细菌
Pub Date : 2017-09-11 DOI: 10.4172/2155-9597.1000319
K. Djenadi
The aim of the current investigation was to look on the presence of the antibiotic resistance bacteria in natural environment in Algeria. From fresh water, multidrug resistant bacteria were harvested, and then investigated for their resistance profile and extend spectrum Beta lactamase and metallo Beta lactamase production. From isolate bacteria only Aeromonas hydrophila catch our attention, because of their metallo Beta lactamase production and alimentary and clinical impact. This finding confirmed our hypothesis that natural environment could be colonized by multidrug resistant bacteria especially to carbapenem antibiotic who may be induced by mutation or horizontal gene transfer. The spread of these kinds of resistance organisms may become a serious issue for the public health and food security.
本次调查的目的是了解阿尔及利亚自然环境中抗生素耐药菌的存在情况。从淡水中收集多药耐药菌,研究其耐药谱、β -内酰胺酶广谱和金属β -内酰胺酶产量。从分离的细菌中,只有嗜水气单胞菌引起了我们的注意,因为它们的金属β -内酰胺酶产生和消化和临床影响。这一发现证实了我们的假设,即自然环境可能被多药耐药细菌定植,特别是碳青霉烯类抗生素,这些细菌可能是由突变或水平基因转移引起的。这些耐药生物的传播可能成为公共卫生和粮食安全的严重问题。
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引用次数: 7
期刊
Journal of Bacteriology & Parasitology
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