Pub Date : 2020-01-01DOI: 10.35248/2155-9597.20.S6.003
Jeremy Walton
The majority of the wording used to characterize the host-microorganism connection has been being used for almost a century. From the get-go in this period, organisms were believed to be essential aggressors that administered the host-microbe connection, bringing about sickness. Afterward, new data about the traits of organisms and their hosts brought about the arrangement that the host-microorganism communication doesn't generally bring about illness. This acknowledgment, thus, prompted the acquaintance of terms with clarify states in which organisms exist inside hosts without causing obvious illness and why a few microorganisms just purpose sickness in specific hosts. Commensal, transporter state, and entrepreneur were terms advanced to represent microorganisms and conditions that were now and then connected with illness yet for which Koch's proposes couldn't be satisfied for some explanation. The majority of these terms were initially proposed to depict the conduct of specific microorganisms, as opposed to characterize a more broad host-organism relationship.
{"title":"Host-Pathogen Interactions: Basic Concepts of Microbial Pathogenesis and Lexicon","authors":"Jeremy Walton","doi":"10.35248/2155-9597.20.S6.003","DOIUrl":"https://doi.org/10.35248/2155-9597.20.S6.003","url":null,"abstract":"The majority of the wording used to characterize the host-microorganism connection has been being used for almost a century. From the get-go in this period, organisms were believed to be essential aggressors that administered the host-microbe connection, bringing about sickness. Afterward, new data about the traits of organisms and their hosts brought about the arrangement that the host-microorganism communication doesn't generally bring about illness. This acknowledgment, thus, prompted the acquaintance of terms with clarify states in which organisms exist inside hosts without causing obvious illness and why a few microorganisms just purpose sickness in specific hosts. Commensal, transporter state, and entrepreneur were terms advanced to represent microorganisms and conditions that were now and then connected with illness yet for which Koch's proposes couldn't be satisfied for some explanation. The majority of these terms were initially proposed to depict the conduct of specific microorganisms, as opposed to characterize a more broad host-organism relationship.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"47 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79354423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.35248/2155-9597.20.S7.E003
Trevor J. Hancock
Iron-oxidizing bacteria are chemotrophic bacteria that derive the energy they need to live and multiply by oxidizing dissolved ferrous iron. They are known to grow and proliferate in waters containing iron concentrations as low as 0.1 mg/L. However, at any rate 0.3 ppm of disintegrated oxygen is needed to carry out oxidation. Iron is a significant component required by living organisms to carry out numerous metabolic reactions such as formation of proteins involved in biochemical reactions, like iron-sulfur proteins, haemoglobin and coordination complexes. This element has a widespread distribution in the planet and is considered as one of the most abundant in the Earth's crust, soil and sediments. Iron is one of the trace elements in marine environments. Its role in the digestion of some chemolithotrophs is probably ancient.
{"title":"A Study of Iron-Oxidizing Bacteria","authors":"Trevor J. Hancock","doi":"10.35248/2155-9597.20.S7.E003","DOIUrl":"https://doi.org/10.35248/2155-9597.20.S7.E003","url":null,"abstract":"Iron-oxidizing bacteria are chemotrophic bacteria that derive the energy they need to live and multiply by oxidizing dissolved ferrous iron. They are known to grow and proliferate in waters containing iron concentrations as low as 0.1 mg/L. However, at any rate 0.3 ppm of disintegrated oxygen is needed to carry out oxidation. Iron is a significant component required by living organisms to carry out numerous metabolic reactions such as formation of proteins involved in biochemical reactions, like iron-sulfur proteins, haemoglobin and coordination complexes. This element has a widespread distribution in the planet and is considered as one of the most abundant in the Earth's crust, soil and sediments. Iron is one of the trace elements in marine environments. Its role in the digestion of some chemolithotrophs is probably ancient.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"134 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75513228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.35248/2155-9597.20.S7.E001
S. Stein
A parasite is a daily routine structure that experiences on or inside a host life form and gets its food from the cost of its host. There are three rule classes of parasites that can cause infections in people: Protozoa, Helminths, and Ectoparasites. Protozoa are minuscule, single-celled living beings that can be free-living or parasitic in nature. They can duplicate in people, which adds to their endurance and moreover allows genuine illnesses to create from a solitary life form. Transmission of protozoa that live in a human's stomach related framework to another human that normally happens through a fecal-oral course (for instance, tainted food or water or individual to-singular contact). Protozoa that live in the blood or tissue of people are communicated to different people by an arthropod vector (for instance, through the nibble of a mosquito or sand fly).
{"title":"CLASSES & Diagnosis of Parasitic Diseases","authors":"S. Stein","doi":"10.35248/2155-9597.20.S7.E001","DOIUrl":"https://doi.org/10.35248/2155-9597.20.S7.E001","url":null,"abstract":"A parasite is a daily routine structure that experiences on or inside a host life form and gets its food from the cost of its host. There are three rule classes of parasites that can cause infections in people: Protozoa, Helminths, and Ectoparasites. Protozoa are minuscule, single-celled living beings that can be free-living or parasitic in nature. They can duplicate in people, which adds to their endurance and moreover allows genuine illnesses to create from a solitary life form. Transmission of protozoa that live in a human's stomach related framework to another human that normally happens through a fecal-oral course (for instance, tainted food or water or individual to-singular contact). Protozoa that live in the blood or tissue of people are communicated to different people by an arthropod vector (for instance, through the nibble of a mosquito or sand fly).","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"29 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81830008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.35248/2155-9597.20.S5.001
V. Payne, Munjam Beltus Dayebga, Yamssi Cédric, N. A. C. Nadia
Background: Malaria is the highest cause of the high infant mortality experienced in Africa, killing 1 in 20 children below the age of 5 years and indeed, killing one child every 30 seconds. The aim of this study was to determine the prevalence of malaria among school children in Bambili. Methods: Blood samples were collected from 800 subjects all over Bambili, some of them through the health centre to ensure a random selection of subjects since patients all over Bambili consult at the health center. Blood samples were collected from respondents and treated appropriately before mounting on glass slides for microscopic examinations using a light microscope. Results: Out of these infections screened in 800 persons, only malaria was recorded in 73 persons (9.13%). The highest infection rate (19.23%) was recorded in October and the lowest (1.54%) in January. Risk factors include seasonal changes, the location and hygiene of residences, the level of education, and the non-use of preventive measures. Conclusion: Malaria appear to be of primary concern thus something must be done by health authorities and sectors responsible for public health issues, in order to effectively control these insect-borne diseases and the nuisance they cause.
{"title":"Prevalence of Malaria among School Children in Bambili-Tubah Sub Division, North West Region, Cameroon","authors":"V. Payne, Munjam Beltus Dayebga, Yamssi Cédric, N. A. C. Nadia","doi":"10.35248/2155-9597.20.S5.001","DOIUrl":"https://doi.org/10.35248/2155-9597.20.S5.001","url":null,"abstract":"Background: Malaria is the highest cause of the high infant mortality experienced in Africa, killing 1 in 20 children below the age of 5 years and indeed, killing one child every 30 seconds. The aim of this study was to determine the prevalence of malaria among school children in Bambili. Methods: Blood samples were collected from 800 subjects all over Bambili, some of them through the health centre to ensure a random selection of subjects since patients all over Bambili consult at the health center. Blood samples were collected from respondents and treated appropriately before mounting on glass slides for microscopic examinations using a light microscope. Results: Out of these infections screened in 800 persons, only malaria was recorded in 73 persons (9.13%). The highest infection rate (19.23%) was recorded in October and the lowest (1.54%) in January. Risk factors include seasonal changes, the location and hygiene of residences, the level of education, and the non-use of preventive measures. Conclusion: Malaria appear to be of primary concern thus something must be done by health authorities and sectors responsible for public health issues, in order to effectively control these insect-borne diseases and the nuisance they cause.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"26 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82029142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.35248/2155-9597.20.11.E378
Suhail Ahmad
The Journal of Bacteriology & Parasitology is an open access, peer-reviewed international journal that publishes scientific articles related to all aspects of bacteriology and Parasitology. It includes the topics of all the Micro-organisms, Viruses, Bacterial Ecology, Parasitic Infection, Pathogenic Bacteria, Bacterial toxin, Bacterial genomics, Bacteraemia, Salmonella, Bacterial Diseases,Intestinal parasites, Parasitic Worms, Anthrax, Clostridial infections, Leprosy, Listeriosis, etc.
{"title":"Corona Virus (COVID-19): A Pandemic which is Threat to Human Species","authors":"Suhail Ahmad","doi":"10.35248/2155-9597.20.11.E378","DOIUrl":"https://doi.org/10.35248/2155-9597.20.11.E378","url":null,"abstract":"The Journal of Bacteriology & Parasitology is an open access, peer-reviewed international journal that publishes scientific articles related to all aspects of bacteriology and Parasitology. It includes the topics of all the Micro-organisms, Viruses, Bacterial Ecology, Parasitic Infection, Pathogenic Bacteria, Bacterial toxin, Bacterial genomics, Bacteraemia, Salmonella, Bacterial Diseases,Intestinal parasites, Parasitic Worms, Anthrax, Clostridial infections, Leprosy, Listeriosis, etc.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"18 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73373294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.35248/2155-9597.20.11.379
H. Huff
Given how quickly the virus spread across the globe within a matter of months, it became clear that there was something unique and extreme about the transmission properties of SARS-CoV-2, the virus driving the COVID-19 pandemic. Public health strategies that emphasize rapid transmission prevention are key to reducing the future impact of this virus on lives and economies across the globe until an effective treatment and vaccine are developed.
{"title":"Viral Spread in Absence of Symptoms in the COVID-19 Pandemic: A Need for Revised Transmission Prevention Strategies","authors":"H. Huff","doi":"10.35248/2155-9597.20.11.379","DOIUrl":"https://doi.org/10.35248/2155-9597.20.11.379","url":null,"abstract":"Given how quickly the virus spread across the globe within a matter of months, it became clear that there was something unique and extreme about the transmission properties of SARS-CoV-2, the virus driving the COVID-19 pandemic. Public health strategies that emphasize rapid transmission prevention are key to reducing the future impact of this virus on lives and economies across the globe until an effective treatment and vaccine are developed.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"12 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75409215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.35248/2155-9597.20.11.367
Fabusiwa Of, Adesina Fp, M. Eganni, Adeomi Aa, Gbenga-Fabusiwa Fj
Background: This research work was carried out at Oka-Akoko, the Headquarters of Akoko South West Local Government Area of Ondo State and its objective is to evaluate the Knowledge on Lassa fever among Primary Health Care workers in Oka-Akoko, Akoko South West Local Government Area, Ondo State Nigeria. Methods: The study design adopted for this work was based on cross sectional descriptive study. The Primary Health Care workers working in government owned Primary Health Care facilities in Oka – Akoko are the study population. Questionnaire was used for the purpose of data collection from the respondents for the investigation. Two hundred and seventy three (273) respondents were selected as a sample size for the study and 250 questionnaires were returned. Result: The mean age of respondents was 35.67 years and 103 (41.2%) have been working for almost 20 years. Half of the respondents 126 (50.4%) agreed that they have attended training or sensitization workshop on Lassa Fever and 162 (64.8%) said that Lassa Fever is a common deadly disease while 156 (62.4%) claimed that Lassa fever is an acute Viral Hemorrhagic illness. Larger percent 218 (87.2%) of the respondents agreed that the direct contact with urine or feaces of infected rats (through food, drinks and touch). Also, high proportion 212 (84.8%) claimed that fever, malaise, headache, muscle pain, vomiting and diarrhea as the signs and symptoms of lassa fever. Conclusion: All the tested variables against overall knowledge on Lassa fever were not significantly associated with the respondents knowledge on lassa fever with p value>0.05.
{"title":"Knowledge on Lassa Fever among Primary Health Care workers in Oka-Akoko, Akoko South West Local Government Area, Ondo State Nigeria","authors":"Fabusiwa Of, Adesina Fp, M. Eganni, Adeomi Aa, Gbenga-Fabusiwa Fj","doi":"10.35248/2155-9597.20.11.367","DOIUrl":"https://doi.org/10.35248/2155-9597.20.11.367","url":null,"abstract":"Background: This research work was carried out at Oka-Akoko, the Headquarters of Akoko South West Local Government Area of Ondo State and its objective is to evaluate the Knowledge on Lassa fever among Primary Health Care workers in Oka-Akoko, Akoko South West Local Government Area, Ondo State Nigeria. Methods: The study design adopted for this work was based on cross sectional descriptive study. The Primary Health Care workers working in government owned Primary Health Care facilities in Oka – Akoko are the study population. Questionnaire was used for the purpose of data collection from the respondents for the investigation. Two hundred and seventy three (273) respondents were selected as a sample size for the study and 250 questionnaires were returned. Result: The mean age of respondents was 35.67 years and 103 (41.2%) have been working for almost 20 years. Half of the respondents 126 (50.4%) agreed that they have attended training or sensitization workshop on Lassa Fever and 162 (64.8%) said that Lassa Fever is a common deadly disease while 156 (62.4%) claimed that Lassa fever is an acute Viral Hemorrhagic illness. Larger percent 218 (87.2%) of the respondents agreed that the direct contact with urine or feaces of infected rats (through food, drinks and touch). Also, high proportion 212 (84.8%) claimed that fever, malaise, headache, muscle pain, vomiting and diarrhea as the signs and symptoms of lassa fever. Conclusion: All the tested variables against overall knowledge on Lassa fever were not significantly associated with the respondents knowledge on lassa fever with p value>0.05.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"1 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90384698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.35248/2155-9597.20.11.E369
S. Gholizadeh
Bacteriology is the conventional branch of Microbiology, which focuses on basic microbiology, host-pathogen interactions, biochemistry, molecular biology and mechanisms, ecology and epidemiology of bacteria. Parasitology is a closest relative of Medical Microbiology that encompasses the parasite world ranging from protozoans to helminthes. The Journal of Bacteriology & Parasitology is an open access, peer-reviewed international journal that publishes scientific articles related to all aspects of bacteriology and parasitology, It includes the topics of Bacterial Ecology, Parasitic Infection, Pathogenic Bacteria, Bacterial toxin, Bacterial genomics, Bacteraemia, Salmonella, Bacterial Diseases, Intestinal parasites, Parasitic Worms, Anthrax, Clostridial infections, Leprosy, Listeriosis, etc. The volume 10 has various aspects of bacteriology & parasitology discussed by the authors from different parts of the world. In the research article, Vu Quang Huy, et al. Trial samples for specific serodiagnosis of anti-Fasciola gigantica antibodies via external quality assessment can be produced with homogeneity and stability lasting for 24 weeks by freeze-drying and freezing methods [1]. Waleed Abu Al-Soud in his research article has done the Detection of the Panton-Valentine Leukocidin Gene in Swedish Isolates of Methicillin-Resistant Staphylococcus aureus using a Multiplex PCR Assay [2]. Arthur Hinton, et al. investigated the use of nBPW will improve verification testing and increase the confidence of FSIS in the results of the Agency’s Salmonella testing data, thus enhancing the ability of the Agency to protect consumers [3]. Linder R et al. have demonstrated about the cooperative (or synergistic) hemolysis, the ability of two bacterial species to jointly lyse erythrocytes, has long been recognized as a helpful tool in the identification of common pathogens (i.e. the CAMP reaction between Streptococcus agalactiae and Staphylococcus aureus) [4]. Halimatou Diop-Ndiaye, et al. concluded that M. genitalium appeared as a second most common STI pathogen identified in patients attending a private laboratory, indicating the need to include its routine detection for STI suffering patients also in public health sector in his research article [5]. Stephane Lepretre, et al. reported a case report a case of a 27year-old patient with severe aplastic anemia who developed Trichosporon inkin sepsis with skin lesions during aplasia after myeloablative allogeneic stem cell transplant [6].
细菌学是微生物学的传统分支，主要研究基础微生物学、宿主-病原体相互作用、生物化学、分子生物学及其机制、细菌生态学和流行病学。寄生虫学是医学微生物学的一个近亲，它涵盖了从原生动物到蠕虫的寄生虫世界。《细菌学与寄生虫学杂志》是一本开放获取、同行评审的国际期刊，发表与细菌学和寄生虫学各方面有关的科学文章，包括细菌生态学、寄生虫感染、致病菌、细菌毒素、细菌基因组学、菌血症、沙门氏菌、细菌疾病、肠道寄生虫、寄生虫、炭疽、梭菌感染、麻风病、李斯特菌病等主题。第10卷有来自世界不同地区的作者讨论的细菌学和寄生虫学的各个方面。在研究文章中，吴广辉等。通过外部质量评估，用于巨型片形吸虫抗体特异性血清诊断的试验样品可通过冷冻干燥和冷冻方法制备，其均匀性和稳定性可持续24周[1]。Waleed Abu Al-Soud在其研究文章中使用多重PCR法检测了瑞典耐甲氧西林金黄色葡萄球菌中潘顿-瓦伦丁嗜白细胞素基因[2]。Arthur Hinton等人调查发现，使用nBPW可以改进验证测试，增加FSIS对机构沙门氏菌检测数据结果的信心，从而增强机构保护消费者的能力[3]。Linder R等人已经证明了合作(或协同)溶血，即两种细菌共同溶血红细胞的能力，长期以来被认为是鉴定常见病原体的有用工具(如无乳酸链球菌和金黄色葡萄球菌之间的CAMP反应)[4]。Halimatou Diop-Ndiaye等人在其研究文章中得出结论，生殖道支原体是在私立实验室就诊的患者中发现的第二大常见性传播感染病原体，这表明需要在公共卫生部门对性病患者进行常规检测[5]。Stephane Lepretre等报道了一例27岁的严重再生障碍性贫血患者，在清骨髓同种异体干细胞移植后，在发育不全过程中出现了三磷酸丝氨酸蛋白脓毒症并伴有皮肤病变[6]。
{"title":"Bacteriology and Parasitology Leads the Way to Origination in Microbiology","authors":"S. Gholizadeh","doi":"10.35248/2155-9597.20.11.E369","DOIUrl":"https://doi.org/10.35248/2155-9597.20.11.E369","url":null,"abstract":"Bacteriology is the conventional branch of Microbiology, which focuses on basic microbiology, host-pathogen interactions, biochemistry, molecular biology and mechanisms, ecology and epidemiology of bacteria. Parasitology is a closest relative of Medical Microbiology that encompasses the parasite world ranging from protozoans to helminthes. The Journal of Bacteriology & Parasitology is an open access, peer-reviewed international journal that publishes scientific articles related to all aspects of bacteriology and parasitology, It includes the topics of Bacterial Ecology, Parasitic Infection, Pathogenic Bacteria, Bacterial toxin, Bacterial genomics, Bacteraemia, Salmonella, Bacterial Diseases, Intestinal parasites, Parasitic Worms, Anthrax, Clostridial infections, Leprosy, Listeriosis, etc. The volume 10 has various aspects of bacteriology & parasitology discussed by the authors from different parts of the world. In the research article, Vu Quang Huy, et al. Trial samples for specific serodiagnosis of anti-Fasciola gigantica antibodies via external quality assessment can be produced with homogeneity and stability lasting for 24 weeks by freeze-drying and freezing methods [1]. Waleed Abu Al-Soud in his research article has done the Detection of the Panton-Valentine Leukocidin Gene in Swedish Isolates of Methicillin-Resistant Staphylococcus aureus using a Multiplex PCR Assay [2]. Arthur Hinton, et al. investigated the use of nBPW will improve verification testing and increase the confidence of FSIS in the results of the Agency’s Salmonella testing data, thus enhancing the ability of the Agency to protect consumers [3]. Linder R et al. have demonstrated about the cooperative (or synergistic) hemolysis, the ability of two bacterial species to jointly lyse erythrocytes, has long been recognized as a helpful tool in the identification of common pathogens (i.e. the CAMP reaction between Streptococcus agalactiae and Staphylococcus aureus) [4]. Halimatou Diop-Ndiaye, et al. concluded that M. genitalium appeared as a second most common STI pathogen identified in patients attending a private laboratory, indicating the need to include its routine detection for STI suffering patients also in public health sector in his research article [5]. Stephane Lepretre, et al. reported a case report a case of a 27year-old patient with severe aplastic anemia who developed Trichosporon inkin sepsis with skin lesions during aplasia after myeloablative allogeneic stem cell transplant [6].","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"21 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77307444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.35248/2155-9597.19.10.363
H. Diop-Ndiaye, R. Jaber, E. Macondo, R. Diagne, Ndiaye Ajs, Diakhaby Eb, A. Sow-Ndoye, M. Camara, A. Ba-Diallo, A. Dieng, T. Siby, Boye Csb, Coumba Touré‐Kâne
Introduction: The contribution of M. genitalium in genital infections in Senegal is poorly understood due to a limited access to molecular biology platforms. The aim of this study is to document the place of M. genitalium infection in men and women attending a private laboratory and to document its association with other STI pathogens. Materials and methods: M. genitalium detection was performed in genital secretions using RealLine Chlamydia trachomatis/ Mycoplasma genitalium (Biosynex, France) at BIO24 biomedical laboratory in Dakar (Senegal). In parallel, other genital pathogens including N. gonorrhoeae and common Mycoplasma species were detected through culture and microscopic analysis. Results: From March 2016 to November 2017, genital secretions from 3550 patients were analyzed. The mean age was 32 years (range: 16 to 71) with a sex-ratio of 0.13. Overall, M. genitalium, C. trachomatis, N. gonorrhoeae and U. urealyticum were detected at a frequency of 1.7%, 2.82%, 0.5% and 11.86%, respectively. In women, C. albicans, bacterial vaginosis, T. vaginalis and M. hominis were found at a frequency of 19.6%, at 22%, 0.8% and 0.9%, respectively. M. genitalium infection was significantly more prevalent in men than women and more frequently associated with C. trachomatis than N. gonorrhoeae. One third of M. genitalium infected women presented also bacterial vaginosis signs and a high pH value (>4.5) of genital secretions was observed in all infected women. Conclusion: M. genitalium appeared as a second most common STI pathogen identified in patients attending a private laboratory, indicating the need to include its routine detection for STI suffering patients also in public health sector.
{"title":"Mycoplasma genitalium in Senegalese Patients Attending a Private Laboratory in Dakar","authors":"H. Diop-Ndiaye, R. Jaber, E. Macondo, R. Diagne, Ndiaye Ajs, Diakhaby Eb, A. Sow-Ndoye, M. Camara, A. Ba-Diallo, A. Dieng, T. Siby, Boye Csb, Coumba Touré‐Kâne","doi":"10.35248/2155-9597.19.10.363","DOIUrl":"https://doi.org/10.35248/2155-9597.19.10.363","url":null,"abstract":"Introduction: The contribution of M. genitalium in genital infections in Senegal is poorly understood due to a limited access to molecular biology platforms. The aim of this study is to document the place of M. genitalium infection in men and women attending a private laboratory and to document its association with other STI pathogens. Materials and methods: M. genitalium detection was performed in genital secretions using RealLine Chlamydia trachomatis/ Mycoplasma genitalium (Biosynex, France) at BIO24 biomedical laboratory in Dakar (Senegal). In parallel, other genital pathogens including N. gonorrhoeae and common Mycoplasma species were detected through culture and microscopic analysis. Results: From March 2016 to November 2017, genital secretions from 3550 patients were analyzed. The mean age was 32 years (range: 16 to 71) with a sex-ratio of 0.13. Overall, M. genitalium, C. trachomatis, N. gonorrhoeae and U. urealyticum were detected at a frequency of 1.7%, 2.82%, 0.5% and 11.86%, respectively. In women, C. albicans, bacterial vaginosis, T. vaginalis and M. hominis were found at a frequency of 19.6%, at 22%, 0.8% and 0.9%, respectively. M. genitalium infection was significantly more prevalent in men than women and more frequently associated with C. trachomatis than N. gonorrhoeae. One third of M. genitalium infected women presented also bacterial vaginosis signs and a high pH value (>4.5) of genital secretions was observed in all infected women. Conclusion: M. genitalium appeared as a second most common STI pathogen identified in patients attending a private laboratory, indicating the need to include its routine detection for STI suffering patients also in public health sector.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"29 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81687403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.35248/2155-9597.19.10.355
M. A. González-López, C. Sánchez-Cruz, J. J. Olivares-Trejo
Introduction: Helicobacter pylori is a gram-negative spiral bacterial, has been associated with peptic ulcers, gastritis, duodenitis, and is believed to be the causative agent of gastric cancer. The sources such as human lactoferrin, haem and haemoglobin can support the H. pylori growth. However, still not fully understood how the process of iron acquisition occurs. An in silico analysis has shown that H. pylori genome has a family of three outer membrane protein regulated by iron (FrpB). Two of them: FrpB1 and FrpB2 were purified as recombinant proteins and their haem- or haemoglobin-binding capability was demonstrated. Unfortunately the last protein of the family (FrpB3) has not been investigated. Methods: In this work FrpB3 was purified by haem-affinity chromatography and its capacity of haem-binding was analyzed. This protein was identified by mass spectrometry and its expression was quantified by real time technique under different human iron sources. This expression was compared with frpB1 and frpb2. The FrpB3 structure was analyzed by 3D model to view the motifs necessary for Hb-binding, and also was compared with FrpB1 and FrpB2 structures. Results: The protein identified was FrpB3, its respective gene was overexpressed with haemoglobin. FrpB1 was overexpressed with haem while FrpB2 was induced in presence of haem and also haemoglobin. Both 3D models showed that they are structurally conserved because they have the typical barrel structure, which is inserted in membrane, also, the motifs necessary for Hb-binding were identified in all the structures. Conclusion: H. pylori express FrpB1, FrpB2 and FrpB3 proteins to scavenge iron and they are regulated according to availability of iron source, maybe in order to withstand the extreme environment present in the stomach. Our overall results represent the effort to explain the importance of iron acquisition.
{"title":"Identification and Characterization of a Family of Outer Membrane Proteins of Helicobacter pylori, which Scavenge Iron from Human Sources","authors":"M. A. González-López, C. Sánchez-Cruz, J. J. Olivares-Trejo","doi":"10.35248/2155-9597.19.10.355","DOIUrl":"https://doi.org/10.35248/2155-9597.19.10.355","url":null,"abstract":"Introduction: Helicobacter pylori is a gram-negative spiral bacterial, has been associated with peptic ulcers, gastritis, duodenitis, and is believed to be the causative agent of gastric cancer. The sources such as human lactoferrin, haem and haemoglobin can support the H. pylori growth. However, still not fully understood how the process of iron acquisition occurs. An in silico analysis has shown that H. pylori genome has a family of three outer membrane protein regulated by iron (FrpB). Two of them: FrpB1 and FrpB2 were purified as recombinant proteins and their haem- or haemoglobin-binding capability was demonstrated. Unfortunately the last protein of the family (FrpB3) has not been investigated. Methods: In this work FrpB3 was purified by haem-affinity chromatography and its capacity of haem-binding was analyzed. This protein was identified by mass spectrometry and its expression was quantified by real time technique under different human iron sources. This expression was compared with frpB1 and frpb2. The FrpB3 structure was analyzed by 3D model to view the motifs necessary for Hb-binding, and also was compared with FrpB1 and FrpB2 structures. Results: The protein identified was FrpB3, its respective gene was overexpressed with haemoglobin. FrpB1 was overexpressed with haem while FrpB2 was induced in presence of haem and also haemoglobin. Both 3D models showed that they are structurally conserved because they have the typical barrel structure, which is inserted in membrane, also, the motifs necessary for Hb-binding were identified in all the structures. Conclusion: H. pylori express FrpB1, FrpB2 and FrpB3 proteins to scavenge iron and they are regulated according to availability of iron source, maybe in order to withstand the extreme environment present in the stomach. Our overall results represent the effort to explain the importance of iron acquisition.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83756969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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