Journal of Asia-pacific Entomology最新文献_第5页

Characterization and molecular docking studies of antioxidant compounds from Malaysian Heterotrigona itama propolis extract 马来西亚异三角蜂蜂胶提取物抗氧化化合物的表征及分子对接研究

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-12-01 Epub Date: 2025-11-05 DOI: 10.1016/j.aspen.2025.102501

Afzan Mahmad , Lee Suan Chua , Lay Jing Seow , Poh Yen Khor , Teh Ubaidah Noh

Propolis from the Malaysian stingless bee Heterotrigona itama (H. itama) has been widely recognized for its antioxidant potential; however, its detailed chemical constituents and possible molecular interactions have not been comprehensively studied. In this study, ethanol extraction and mild alkaline hydrolysis were used to prepare H. itama propolis extracts, which were characterized using ATR–FTIR, GC–MS, and UHPLC–MS/MS techniques. The radical scavenging activities of the compounds were evaluated using ABTS and hydrogen peroxide scavenging assays. The total flavonoid content was 11.88 ± 0.10 mg QE/g for the crude extract (F1) and increased to 14.79 ± 0.08 mg QE/g for the hydrolyzed extract (F2), while the total phenolic content rose from 0.83 ± 0.17 mg GAE/g (F1) to 1.21 ± 0.09 mg GAE/g (F2), indicating the enhanced release of bioactive constituents. Functional group and compound profiling confirmed the presence of diverse phenolics (including flavonoids), terpenoids (including limonoids) and sterols. Notably, the identified compounds such as oxyphyllacinol, licarin B, evobioside, kushenol B, and nimbolidin C are known for their antioxidant activities. Molecular docking results indicated that five phytochemicals from H. itama propolis extract were predicted to bind stably to COX–2 (–5.7 to –8.2 kcal/mol), and strong antioxidant capacities were demonstrated by ABTS (∼90 %) and hydrogen peroxide scavenging assays (54.84–69.73 %). Based on these findings, it is demonstrated that H. itama propolis extract represents a rich natural antioxidant source with promising bioactivity. Further in vivo validation, pharmacokinetic, and toxicological studies are recommended to confirm the therapeutic applicability and safety of these compounds in pharmaceutical and nutraceutical products.

马来西亚无刺蜂（Heterotrigona itama， H. itama）的蜂胶因其抗氧化潜力而被广泛认可；然而，其详细的化学成分和可能的分子相互作用尚未得到全面的研究。本研究采用乙醇提取和温和碱性水解法制备羽绒蜂胶提取物，并采用ATR-FTIR、GC-MS和UHPLC-MS /MS技术对其进行了表征。采用ABTS和过氧化氢清除法对化合物的自由基清除活性进行了评价。粗提物（F1）总黄酮含量为11.88±0.10 mg QE/g，水解提物（F2）总黄酮含量为14.79±0.08 mg QE/g，总酚含量从0.83±0.17 mg GAE/g （F1）增加到1.21±0.09 mg GAE/g (F2)，表明活性成分释放增强。官能团和化合物谱证实了多种酚类物质（包括黄酮类）、萜类物质（包括柠檬酮）和甾醇的存在。值得注意的是，已鉴定的化合物，如叶香树醇、李卡林B、左旋草苷、苦参酚B和莲叶素C，以其抗氧化活性而闻名。分子对接结果表明，itama蜂胶提取物中的5种植物化学物质与COX-2稳定结合（-5.7 ~ -8.2 kcal/mol）， ABTS（~ 90%）和过氧化氢清除（54.84 ~ 69.73%）显示出较强的抗氧化能力。综上所述，itama蜂胶提取物是一种丰富的天然抗氧化来源，具有良好的生物活性。建议进行进一步的体内验证、药代动力学和毒理学研究，以确认这些化合物在药物和保健品中的治疗适用性和安全性。

{"title":"Characterization and molecular docking studies of antioxidant compounds from Malaysian Heterotrigona itama propolis extract","authors":"Afzan Mahmad , Lee Suan Chua , Lay Jing Seow , Poh Yen Khor , Teh Ubaidah Noh","doi":"10.1016/j.aspen.2025.102501","DOIUrl":"10.1016/j.aspen.2025.102501","url":null,"abstract":"<div><div>Propolis from the Malaysian stingless bee Heterotrigona itama (H. itama) has been widely recognized for its antioxidant potential; however, its detailed chemical constituents and possible molecular interactions have not been comprehensively studied. In this study, ethanol extraction and mild alkaline hydrolysis were used to prepare H. itama propolis extracts, which were characterized using ATR–FTIR, GC–MS, and UHPLC–MS/MS techniques. The radical scavenging activities of the compounds were evaluated using ABTS and hydrogen peroxide scavenging assays. The total flavonoid content was 11.88 ± 0.10 mg QE/g for the crude extract (F1) and increased to 14.79 ± 0.08 mg QE/g for the hydrolyzed extract (F2), while the total phenolic content rose from 0.83 ± 0.17 mg GAE/g (F1) to 1.21 ± 0.09 mg GAE/g (F2), indicating the enhanced release of bioactive constituents. Functional group and compound profiling confirmed the presence of diverse phenolics (including flavonoids), terpenoids (including limonoids) and sterols. Notably, the identified compounds such as oxyphyllacinol, licarin B, evobioside, kushenol B, and nimbolidin C are known for their antioxidant activities. Molecular docking results indicated that five phytochemicals from <em>H. itama</em> propolis extract were predicted to bind stably to COX–2 (–5.7 to –8.2 kcal/mol), and strong antioxidant capacities were demonstrated by ABTS (∼90 %) and hydrogen peroxide scavenging assays (54.84–69.73 %). Based on these findings, it is demonstrated that H. itama propolis extract represents a rich natural antioxidant source with promising bioactivity. Further in vivo validation, pharmacokinetic, and toxicological studies are recommended to confirm the therapeutic applicability and safety of these compounds in pharmaceutical and nutraceutical products.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102501"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145516479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible interactive risk of copper hydroxide, inorganic bactericide of fire blight with dinotefuran and triflumizole on honey bee 氢氧化铜、无机杀菌剂与呋喃、三氟唑对蜜蜂可能的交互作用风险

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-12-01 Epub Date: 2025-09-06 DOI: 10.1016/j.aspen.2025.102475

Hyeonjeong Jang , Tekalign Begna , Sukjun Sun , Delgermaa Ulziibayar , Chuleui Jung

In spring 2021, abnormal honey bee mortality was observed near apple orchards in Cheongsong, South Korea, following copper-based fire blight control. This study evaluated the acute toxicity of copper hydroxide and basic copper sulfate to honey bees, assessed residue levels in field-collected bees, and investigated potential synergistic effects with co-occurring pesticides. Five-step serial dilutions of the recommended field concentration (RFC) were used for acute oral and contact toxicity assays. ICP-OES was used to quantify copper residues in lab-exposed and field-collected bees, while multi-residue pesticide analysis was performed with LC-MS/MS and GC–MS/MS. Copper hydroxide showed the highest oral toxicity, with both LD₅₀ and HQ values indicating moderate toxicity. Field-collected bees exhibited higher copper bioaccumulation than control, comparable to lab-exposed bees with a 10⁻² RFC dilution, which remained well under the LC₅₀. This implies that copper alone is not the cause of the witnessed phenomenon. Among detected pesticides, dinotefuran exhibited high HQ values, exceeding elevated risk threshold. Triflumizole and buprofezin showed minimal risk; however, triflumizole was considered likely to interact with dinotefuran, with their short half-lives potentially contributing to increased toxicity in bees. The combination of copper, dinotefuran, and triflumizole suggests potential interactive toxicity contributing to the observed mortality. These findings underscore the need to account for pesticide mixtures in practical management strategies, as even low-risk compounds can contribute to elevated toxicity when applied together. To prevent unintended harm to honey bees, a more integrated and pollinator-conscious approach to pesticide use is essential.

2021年春季，在韩国青松市的苹果园附近，在铜基火疫病防治之后，观察到蜜蜂死亡率异常。本研究评估了氢氧化铜和碱式硫酸铜对蜜蜂的急性毒性，评估了现场采集蜜蜂的残留水平，并研究了它们与共效农药的潜在协同效应。五步连续稀释推荐场浓度（RFC）用于急性口服和接触毒性试验。采用ICP-OES定量实验室接触和野外采集的蜜蜂体内铜残留，采用LC-MS/MS和GC-MS /MS进行多残留农药分析。氢氧化铜的口服毒性最高，LD50和HQ值均为中等毒性。野外采集的蜜蜂表现出比对照更高的铜生物积累，与实验室暴露的蜜蜂相比，RFC稀释为10−2，但仍保持在LC50以下。这意味着铜本身并不是所见现象的原因。在检测到的农药中，敌敌畏的HQ值较高，超过了风险升高阈值。三氟唑和丁丙非津风险最小；然而，三氟唑被认为可能与呋虫胺相互作用，它们的半衰期较短，可能会增加蜜蜂的毒性。铜、呋虫胺和三氟唑的联合作用提示潜在的相互作用毒性导致观察到的死亡率。这些发现强调了在实际管理策略中考虑农药混合物的必要性，因为即使是低风险化合物在一起使用时也可能导致毒性升高。为了防止对蜜蜂造成意外伤害，对农药的使用采取一种更综合、更注重传粉媒介的方法至关重要。

{"title":"Possible interactive risk of copper hydroxide, inorganic bactericide of fire blight with dinotefuran and triflumizole on honey bee","authors":"Hyeonjeong Jang , Tekalign Begna , Sukjun Sun , Delgermaa Ulziibayar , Chuleui Jung","doi":"10.1016/j.aspen.2025.102475","DOIUrl":"10.1016/j.aspen.2025.102475","url":null,"abstract":"<div><div>In spring 2021, abnormal honey bee mortality was observed near apple orchards in Cheongsong, South Korea, following copper-based fire blight control. This study evaluated the acute toxicity of copper hydroxide and basic copper sulfate to honey bees, assessed residue levels in field-collected bees, and investigated potential synergistic effects with co-occurring pesticides. Five-step serial dilutions of the recommended field concentration (RFC) were used for acute oral and contact toxicity assays. ICP-OES was used to quantify copper residues in lab-exposed and field-collected bees, while multi-residue pesticide analysis was performed with LC-MS/MS and GC–MS/MS. Copper hydroxide showed the highest oral toxicity, with both LD<sub>50</sub> and HQ values indicating moderate toxicity. Field-collected bees exhibited higher copper bioaccumulation than control, comparable to lab-exposed bees with a 10<sup>−2</sup> RFC dilution, which remained well under the LC<sub>50</sub>. This implies that copper alone is not the cause of the witnessed phenomenon. Among detected pesticides, dinotefuran exhibited high HQ values, exceeding elevated risk threshold. Triflumizole and buprofezin showed minimal risk; however, triflumizole was considered likely to interact with dinotefuran, with their short half-lives potentially contributing to increased toxicity in bees. The combination of copper, dinotefuran, and triflumizole suggests potential interactive toxicity contributing to the observed mortality. These findings underscore the need to account for pesticide mixtures in practical management strategies, as even low-risk compounds can contribute to elevated toxicity when applied together. To prevent unintended harm to honey bees, a more integrated and pollinator-conscious approach to pesticide use is essential.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102475"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An effective formulation from Annona squamosa L. Leaves against Aedes aegypti 番槐叶抗埃及伊蚊有效配方研究

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-12-01 Epub Date: 2025-10-13 DOI: 10.1016/j.aspen.2025.102491

Lucas Gabriel Pita dos Santos , Lívia Laís de Santana Silva Barbosa , Gabriel da Silva Coutinho , Emerson de Oliveira Silva , Júlio Cesar Ribeiro de Oliveira Farias de Aguiar , Glaucilane dos Santos Cruz , Alvaro Aguiar Coelho Teixeira , Valéria Wanderley Teixeira , Patrícia Maria Guedes Paiva , Daniela Maria do Amaral Ferraz Navarro , Pedro José Rolim Neto , Thiago Henrique Napoleão , Welton Aaron de Almeida , Emmanuel Viana Pontual

Amid rising resistance to synthetic insecticides and growing environmental concerns, plant-based alternatives have gained relevance in vector control strategies. This study reports the development of a solid tablet formulation derived from the saline extract of Annona squamosa L. (Annonaceae) leaves, which contains cinnamic acid derivatives, rutin, chlorogenic acid, lectins, and trypsin inhibitors. Thermal and spectroscopic analyses (DTA and FTIR) confirmed chemical compatibility between the extract and excipients, supporting the formulation’s physicochemical stability. When dissolved to deliver 1.9 % (w/v) of the extract, the tablet induced 42 ± 14 % mortality in third-instar Aedes aegypti L. (Diptera: Culicidae) larvae. A concentration-dependent larvicidal effect was observed, with an estimated LC₅₀ of 3.10 % and a diagnostic dose of 2.20 % (w/v). Histological examination revealed severe midgut disruption in treated larvae, including vacuolization, epithelial hypertrophy, and regenerative cell proliferation—hallmarks of necrotic processes. Although effective, the current tablet requires further optimization to enhance larvicidal potency. Additional studies are also needed to assess environmental safety and real-world performance, reinforcing the potential of this tablet as a sustainable larvicide for integrated mosquito management.

在对合成杀虫剂的抗药性日益增强和环境问题日益严重的情况下，基于植物的替代品已在病媒控制战略中获得了相关性。本研究报告了一种固体片剂制剂的开发，该制剂是从番槐科植物番槐叶的生理盐水提取物中提取的，其中含有肉桂酸衍生物、芦丁、绿原酸、凝集素和胰蛋白酶抑制剂。热分析和光谱分析（DTA和FTIR）证实了提取物和辅料之间的化学相容性，支持配方的物理化学稳定性。当溶出浓度为1.9% （w/v）时，该片剂对埃及伊蚊（Aedes aegypti L.） 3龄幼虫的死亡率为42±14%。观察到浓度依赖性的杀幼虫效果，估计LC₅0为3.10%，诊断剂量为2.20% （w/v）。组织学检查显示，处理过的幼虫严重的中肠破坏，包括空泡化、上皮肥大和再生细胞增殖——坏死过程的标志。虽然有效，但目前的片剂需要进一步优化以增强杀幼虫效力。还需要进一步的研究来评估环境安全性和实际性能，以加强这种片剂作为综合蚊子管理的可持续杀幼虫剂的潜力。

{"title":"An effective formulation from Annona squamosa L. Leaves against Aedes aegypti","authors":"Lucas Gabriel Pita dos Santos , Lívia Laís de Santana Silva Barbosa , Gabriel da Silva Coutinho , Emerson de Oliveira Silva , Júlio Cesar Ribeiro de Oliveira Farias de Aguiar , Glaucilane dos Santos Cruz , Alvaro Aguiar Coelho Teixeira , Valéria Wanderley Teixeira , Patrícia Maria Guedes Paiva , Daniela Maria do Amaral Ferraz Navarro , Pedro José Rolim Neto , Thiago Henrique Napoleão , Welton Aaron de Almeida , Emmanuel Viana Pontual","doi":"10.1016/j.aspen.2025.102491","DOIUrl":"10.1016/j.aspen.2025.102491","url":null,"abstract":"<div><div>Amid rising resistance to synthetic insecticides and growing environmental concerns, plant-based alternatives have gained relevance in vector control strategies. This study reports the development of a solid tablet formulation derived from the saline extract of <em>Annona squamosa</em> L. (Annonaceae) leaves, which contains cinnamic acid derivatives, rutin, chlorogenic acid, lectins, and trypsin inhibitors. Thermal and spectroscopic analyses (DTA and FTIR) confirmed chemical compatibility between the extract and excipients, supporting the formulation’s physicochemical stability. When dissolved to deliver 1.9 % (w/v) of the extract, the tablet induced 42 ± 14 % mortality in third-instar <em>Aedes aegypti</em> L. (Diptera: Culicidae) larvae. A concentration-dependent larvicidal effect was observed, with an estimated LC₅₀ of 3.10 % and a diagnostic dose of 2.20 % (w/v). Histological examination revealed severe midgut disruption in treated larvae, including vacuolization, epithelial hypertrophy, and regenerative cell proliferation—hallmarks of necrotic processes. Although effective, the current tablet requires further optimization to enhance larvicidal potency. Additional studies are also needed to assess environmental safety and real-world performance, reinforcing the potential of this tablet as a sustainable larvicide for integrated mosquito management.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102491"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145321158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redescription of Cylindralcides takahashii (Kôno) (Coleoptera, Curculionidae, Molytinae, Mecysolobini) damaged on the Thunberg’s bay-tree 高桥圆柱孢菌（Kôno）（鞘翅目，曲霉科，Molytinae, Mecysolobini）在桐树上的危害再认识

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-12-01 Epub Date: 2025-10-17 DOI: 10.1016/j.aspen.2025.102492

Dilshara D. Wijesinghe , Sangwook Park , Dong-Soon Kim , Ki-Jeong Hong

Fifteen adult and larval specimens of Cylindralcides (s. str.) takahashii (Kôno, 1930), a member of the tribe Mecysolobini (Curculionidae: Molytinae), were collected on new shoots of Thunberg’s bay-tree, Machilus thunbergii Siebold and Zucc. from Jeju Island of Korea. This represents the first record of the genus Cylindralcides Heller, 1918 in Korea and the first documented record of M. thunbergii as its host plant. The original description of C. takahashii was brief and lacked detailed diagnostic features, leading to misidentification. This study provides a redescription with diagnostic features, detailed illustrations of the adult and larva, and ecological observations, aiming to clarify the taxonomic status of the species. Additionally, the 682 bp of COI DNA barcode data support the assessment of molecular relationships within the tribe Mecysolobini.

本文在桐叶月桂树Machilus thunbergii Siebold和Zucc的新枝上，采集了桐叶月桂树（Mecysolobini科：Molytinae）科成员圆柱孢菌（s.str .） takahashi (Kôno, 1930) 15个成虫和幼虫标本。来自韩国济州岛。这是韩国在1918年首次记录到的圆柱孢属植物，也是首次记录到的寄主植物为刺贝属植物。最初对C. takahashi的描述很简短，缺乏详细的诊断特征，导致误认。本研究对该物种进行了诊断特征的重新描述，详细描述了成虫和幼虫，并进行了生态观察，旨在阐明该物种的分类地位。此外，682 bp的COI DNA条形码数据支持了Mecysolobini部落分子关系的评估。

引用次数: 0

Molecular chaperone heat shock protein 70 mediates thermotolerance and insecticide resistance in Aphis glycines Matsumura (Hemiptera: Aphididae) 分子伴侣热休克蛋白70介导松村甘氨酸蚜虫的耐热性和抗药性（半翅目：蚜虫科）

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-12-01 Epub Date: 2025-10-17 DOI: 10.1016/j.aspen.2025.102497

Zhengbing Wang , Haojia Bao , Hongfei Zhang , Manman Zheng , Xiaoyue Sang , Mingsheng Yang , Keshi Ma

The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is a major agricultural pest that causes substantial yield losses in soybean production. Heat shock proteins (Hsps) are evolutionarily conserved molecular chaperones that play critical roles in regulating cellular responses to abiotic and biotic stresses. In this study, we identified an Hsp70 gene (AglyHsp70) from the soybean aphid genome and investigated its expression profile and role in stress tolerance under thermal and insecticide challenges. Sequence alignment and phylogenetic analysis revealed that AglyHsp70 shares strong homology with Hsp70 proteins from other insect species. Developmental expression analysis showed that AglyHsp70 was most abundantly expressed in first-instar nymphs. Under heat stress, AglyHsp70 expression was strongly induced but rapidly returned to baseline levels after recovery from both heat and cold shocks. Exposure to imidacloprid significantly upregulated AglyHsp70 expression in a concentration- and time-dependent manner. Moreover, RNAi-mediated knockdown of AglyHsp70 markedly increased soybean aphid mortality under heat stress and at specific concentrations of imidacloprid. Collectively, these findings demonstrate that AglyHsp70 plays an essential role in stress tolerance against both thermal and chemical stressors in A. glycines. This study provides novel insights into the molecular mechanisms of stress adaptation in soybean aphids and highlights potential molecular targets for sustainable pest management strategies.

大豆蚜虫（半翅目：蚜虫科）是一种主要的农业害虫，对大豆生产造成严重的产量损失。热休克蛋白（Hsps）是进化上保守的分子伴侣，在调节细胞对非生物和生物胁迫的反应中起着关键作用。在这项研究中，我们从大豆蚜虫基因组中鉴定了一个Hsp70基因（AglyHsp70），并研究了其表达谱及其在高温和杀虫剂胁迫下的耐受性中的作用。序列比对和系统发育分析表明，AglyHsp70蛋白与其他昆虫的Hsp70蛋白具有较强的同源性。发育表达分析表明，AglyHsp70在一龄若虫中表达量最高。在热应激下，AglyHsp70的表达被强烈诱导，但在热休克和冷休克恢复后迅速恢复到基线水平。吡虫啉暴露显著上调AglyHsp70的表达，并呈浓度和时间依赖性。此外，rnai介导的AglyHsp70的敲低显著增加了高温胁迫和特定浓度吡虫啉下大豆蚜虫的死亡率。综上所述，这些发现表明，AglyHsp70在A.甘氨酸对热应激和化学应激的耐受性中起着至关重要的作用。该研究为大豆蚜虫适应逆境的分子机制提供了新的见解，并为可持续虫害管理策略提供了潜在的分子靶点。

{"title":"Molecular chaperone heat shock protein 70 mediates thermotolerance and insecticide resistance in Aphis glycines Matsumura (Hemiptera: Aphididae)","authors":"Zhengbing Wang , Haojia Bao , Hongfei Zhang , Manman Zheng , Xiaoyue Sang , Mingsheng Yang , Keshi Ma","doi":"10.1016/j.aspen.2025.102497","DOIUrl":"10.1016/j.aspen.2025.102497","url":null,"abstract":"<div><div>The soybean aphid, <em>Aphis glycines</em> Matsumura (Hemiptera: Aphididae), is a major agricultural pest that causes substantial yield losses in soybean production. Heat shock proteins (Hsps) are evolutionarily conserved molecular chaperones that play critical roles in regulating cellular responses to abiotic and biotic stresses. In this study, we identified an Hsp70 gene (<em>AglyHsp70</em>) from the soybean aphid genome and investigated its expression profile and role in stress tolerance under thermal and insecticide challenges. Sequence alignment and phylogenetic analysis revealed that AglyHsp70 shares strong homology with Hsp70 proteins from other insect species. Developmental expression analysis showed that <em>AglyHsp70</em> was most abundantly expressed in first-instar nymphs. Under heat stress, <em>AglyHsp70</em> expression was strongly induced but rapidly returned to baseline levels after recovery from both heat and cold shocks. Exposure to imidacloprid significantly upregulated <em>AglyHsp70</em> expression in a concentration- and time-dependent manner. Moreover, RNAi-mediated knockdown of <em>AglyHsp70</em> markedly increased soybean aphid mortality under heat stress and at specific concentrations of imidacloprid. Collectively, these findings demonstrate that <em>AglyHsp70</em> plays an essential role in stress tolerance against both thermal and chemical stressors in <em>A. glycines</em>. This study provides novel insights into the molecular mechanisms of stress adaptation in soybean aphids and highlights potential molecular targets for sustainable pest management strategies.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102497"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145358741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insecticidal properties of tea seed saponin against the mulberry snout moth, Glyphodes pyloalis Walker (Lepidoptera: Crambidae) 茶籽皂苷对桑鼻蛾的杀虫性能研究

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-12-01 Epub Date: 2025-08-26 DOI: 10.1016/j.aspen.2025.102465

Samar Ramzi , Ehsan Kahneh , Reza Azadi Gonbad , Fatemeh Parsa , Morteza Shahriari

The mulberry snout moth, Glyphodes pyloalis Walker, is an important and destructive pest of mulberry in Asia. Because of its extensive damages on mulberry leaves and making obstacle in sericulture, it is inevitable application of synthetic and biorational insecticides. Excessive application of insecticides may lead to develop resistance and to pollute environment mainly water resources therefore use of natural products (like botanical insecticides) is of great importance as an alternative. In the present study, a saponin was extracted from tea seeds and treated against G. pyloalis larvae. Results showed that a concentration of 2.8 % saponin caused 50 % mortality in treated larvae compared to the control, and the LT₅₀ (Median Lethal Time) was 6.34 days. Also, the repellency index increased in a dose-dependent manner. Tea saponin treatment increased the duration of egg hatching, larval and pre-pupae periods compared to the control. Activities of digestive carbohydrates and proteases were significantly reduced in the exposed larvae. Additionally, saponin treatment significantly decreased the activity of digestive enzymes but it statistically induced antioxidant system in G. pyloalis larvae. The obtained results suggested that tea saponin can affect important biological and physiological processes of G. pyloalis and it may be used as an efficient biopesticide in control of G. pyloalis population.

桑树鼻蛾（Glyphodes pyloalis Walker）是亚洲桑树的重要危害害虫。由于其对桑叶危害广泛，对桑蚕养殖造成障碍，因此合成和生物杀虫剂的应用是不可避免的。过量使用杀虫剂可能导致抗药性，污染环境，主要是水资源，因此使用天然产品（如植物性杀虫剂）作为替代方法非常重要。本研究从茶叶种子中提取一种皂素，并对其进行防治幽门螺杆菌幼虫的研究。结果表明，浓度为2.8%的皂苷处理的幼虫死亡率为对照组的50%，LT50（中位致死时间）为6.34 d。驱避指数呈剂量依赖性增加。与对照组相比，茶皂素处理增加了卵孵化期、幼虫期和蛹期。暴露后幼虫的消化碳水化合物和蛋白酶活性显著降低。此外，皂素处理显著降低了幽门螺杆菌幼虫的消化酶活性，但对抗氧化系统有显著的促进作用。结果表明，茶皂素可影响幽门螺杆菌的重要生理和生物学过程，可作为一种有效的生物农药防治幽门螺杆菌。

{"title":"Insecticidal properties of tea seed saponin against the mulberry snout moth, Glyphodes pyloalis Walker (Lepidoptera: Crambidae)","authors":"Samar Ramzi , Ehsan Kahneh , Reza Azadi Gonbad , Fatemeh Parsa , Morteza Shahriari","doi":"10.1016/j.aspen.2025.102465","DOIUrl":"10.1016/j.aspen.2025.102465","url":null,"abstract":"<div><div>The mulberry snout moth, <em>Glyphodes pyloalis</em> Walker, is an important and destructive pest of mulberry in Asia. Because of its extensive damages on mulberry leaves and making obstacle in sericulture, it is inevitable application of synthetic and biorational insecticides. Excessive application of insecticides may lead to develop resistance and to pollute environment mainly water resources therefore use of natural products (like botanical insecticides) is of great importance as an alternative. In the present study, a saponin was extracted from tea seeds and treated against <em>G. pyloalis</em> larvae. Results showed that a concentration of 2.8 % saponin caused 50 % mortality in treated larvae compared to the control, and the LT<sub>50</sub> (Median Lethal Time) was 6.34 days. Also, the repellency index increased in a dose-dependent manner. Tea saponin treatment increased the duration of egg hatching, larval and pre-pupae periods compared to the control. Activities of digestive carbohydrates and proteases were significantly reduced in the exposed larvae. Additionally, saponin treatment significantly decreased the activity of digestive enzymes but it statistically induced antioxidant system in <em>G. pyloalis</em> larvae. The obtained results suggested that tea saponin can affect important biological and physiological processes of <em>G. pyloalis</em> and it may be used as an efficient biopesticide in control of <em>G. pyloalis</em> population.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102465"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145221180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Field evidence of Plesiochrysa ramburi (Neuroptera: Chrysopidae) establishment and predation on invasive mealybugs in the Sakishima Islands, Japan 在日本咲岛群岛，野蛾（神经翅目：野蛾科）的建立和捕食入侵粉蚧的野外证据

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-12-01 Epub Date: 2025-09-30 DOI: 10.1016/j.aspen.2025.102488

Masayuki Hayashi , Yuta Owashi , Tatsuya Yoshida

Spreading invasive pest insects cause substantial agricultural damage worldwide. Their natural enemies can also expand their distributions, thus presenting potential opportunities for biological control. The green lacewing Plesiochrysa ramburi (Schneider), widely distributed from Southeast Asia to Australia, Polynesia, and Micronesia, has potential as a natural enemy for agricultural pest control owing to its predation on economically important pest mealybugs. Only a few specimens have been recorded in Japan in recent years, and the species’ ecological characteristics in the region remain poorly understood. Here, we recorded multiple adults and immatures—eggs, larvae, and pupae—of P. ramburi associated with invasive pest mealybugs in the Sakishima Islands, Japan. Collection of the lacewings from multiple sites on Ishigaki Island and their observation on Miyako Island, over 100 km away, confirms widespread establishment in the region. Immature stages were closely associated with colonies of three invasive mealybug species, Paracoccus marginatus, Phenacoccus solenopsis, and Maconellicoccus hirsutus. Larvae of P. ramburi, covered with wax secretions from their prey, were observed preying on the mealybugs. We discuss the potential for P. ramburi to continue expanding its distribution and its potential as a natural enemy for pest control in Japan.

入侵性害虫的传播在世界范围内造成了巨大的农业损失。它们的天敌也可以扩大它们的分布，从而为生物防治提供了潜在的机会。绿草草Plesiochrysa ramburi （Schneider）广泛分布于东南亚至澳大利亚、波利尼西亚和密克罗尼西亚，因其捕食具有重要经济意义的粉蚧而成为农业害虫防治的潜在天敌。近年来，日本只记录了少数标本，该地区物种的生态特征仍然知之甚少。在日本的Sakishima群岛，我们记录了与入侵害虫粉蚧相关的P. ramburi的多个成虫和未成熟虫的卵、幼虫和蛹。从石垣岛多个地点收集草蛉，并在100多公里外的宫古岛观察草蛉，证实在该地区广泛存在。未成熟阶段与三种入侵粉蚧的菌落密切相关，即边缘副球菌、扶桑绵粉球菌和毛毛单胞球菌。观察到被其猎物分泌的蜡状分泌物覆盖的兰布氏夜蛾幼虫捕食粉蚧。我们讨论了在日本继续扩大其分布的潜力及其作为害虫控制天敌的潜力。

{"title":"Field evidence of Plesiochrysa ramburi (Neuroptera: Chrysopidae) establishment and predation on invasive mealybugs in the Sakishima Islands, Japan","authors":"Masayuki Hayashi , Yuta Owashi , Tatsuya Yoshida","doi":"10.1016/j.aspen.2025.102488","DOIUrl":"10.1016/j.aspen.2025.102488","url":null,"abstract":"<div><div>Spreading invasive pest insects cause substantial agricultural damage worldwide. Their natural enemies can also expand their distributions, thus presenting potential opportunities for biological control. The green lacewing <em>Plesiochrysa ramburi</em> (Schneider), widely distributed from Southeast Asia to Australia, Polynesia, and Micronesia, has potential as a natural enemy for agricultural pest control owing to its predation on economically important pest mealybugs. Only a few specimens have been recorded in Japan in recent years, and the species’ ecological characteristics in the region remain poorly understood. Here, we recorded multiple adults and immatures—eggs, larvae, and pupae—of <em>P. ramburi</em> associated with invasive pest mealybugs in the Sakishima Islands, Japan. Collection of the lacewings from multiple sites on Ishigaki Island and their observation on Miyako Island, over 100 km away, confirms widespread establishment in the region. Immature stages were closely associated with colonies of three invasive mealybug species, <em>Paracoccus marginatus</em>, <em>Phenacoccus solenopsis</em>, and <em>Maconellicoccus hirsutus</em>. Larvae of <em>P. ramburi</em>, covered with wax secretions from their prey, were observed preying on the mealybugs. We discuss the potential for <em>P. ramburi</em> to continue expanding its distribution and its potential as a natural enemy for pest control in Japan.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102488"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145221181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can aromatic and medicinal plants effectively control Tylenchulus semipenetrans? Evidence from in vitro and in vivo studies 芳香和药用植物是否能有效防治半透叶菊？来自体外和体内研究的证据

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-12-01 Epub Date: 2025-09-17 DOI: 10.1016/j.aspen.2025.102482

Btissam Zoubi , Fouad Mokrini , Meriem Rafya , Abderrazak Benkebboura , Oumaima Akachoud , Ghizlane Krif , Ghoulam Cherki , Abdelilah Iraqi Housseini , Ahmed Qaddoury

Tylenchulus semipenetrans is a highly damaging obligate plant parasitic nematode (PPN) that poses a significant threat to citrus crops. The use of chemical nematicides has proven effective in reducing T. semipenetrans populations; however, repeated use can have negative environmental impacts, necessitating the adoption of more ecological strategies. The current study aimed to evaluate the nematicidal effect of three aromatic and medicinal plants (AMPs), Rosmarinus officinalis, Laurus nobilis and Verbena officinalis against T. semipenetrans in terms of juvenile J2 mortality and eggs hatch inhibition under greenhouse and laboratory conditions. The results showed that all AMPs aqueous extracts exhibited toxicity against T. semipenetrans, with J2 mortality ranging between 25.85 % and 75.17 %, compared to 1.0 to 13.6 % in the control treatment. Egg hatching varied between 0 % and 66 %, compared to 73 to 95 % in control treatment. Toxicity increased with increasing AMPs aqueous extract concentration and incubation period, reaching more than 55.44 % mortality after 96 h and complete egg hatching inhibition after 144 h of incubation at an R. officinalis aqueous extract concentration of 10 %. In the greenhouse experiment, the aqueous extract of R. officinalis was highly active against T. semipenetrans, resulting in a substantial reduction in nematode populations, with counts ranging from 475 nematodes/100 g of soil and 122 nematodes/10 g of roots, compared to populations of 997.30 nematodes/100 g of soil and 185 nematodes/10 g of roots in the control treatment. The results of this study suggest that AMPs studied, could be used as a bionematicide product for managing T. semipenetrans.

半透叶霉（Tylenchulus semipenetrans）是一种破坏性极强的专性植物寄生线虫（PPN），对柑橘作物构成严重威胁。使用化学杀线虫剂已被证明对减少半透线虫种群有效；然而，重复使用会对环境产生负面影响，因此需要采取更多的生态战略。在温室和实验室条件下，研究了迷香、野月桂和马鞭草3种芳香药用植物对半透蛾的杀线虫效果，包括对幼虫J2的死亡率和对卵孵化的抑制。结果表明，所有AMPs水提液对半透虫均有毒性，J2死亡率为25.85% ~ 75.17%，而对照处理的J2死亡率为1.0 ~ 13.6%。鸡蛋的孵化率在0%到66%之间，而对照处理的孵化率为73%到95%。毒力随AMPs水提物浓度的增加和孵育时间的延长而增加，96 h后死亡率达到55.44%以上，10%的水提物孵育144 h后完全抑制卵孵化。在温室试验中，与对照处理的997.30根/100克土壤和185根线虫的数量相比，石斛水提物对半穿透线虫的活性较高，线虫的数量大幅减少，为475根/100克土壤和122根/10克根系。本研究结果表明，所研究的AMPs可以作为一种生物杀线虫剂用于治疗半透线虫。

{"title":"Can aromatic and medicinal plants effectively control Tylenchulus semipenetrans? Evidence from in vitro and in vivo studies","authors":"Btissam Zoubi , Fouad Mokrini , Meriem Rafya , Abderrazak Benkebboura , Oumaima Akachoud , Ghizlane Krif , Ghoulam Cherki , Abdelilah Iraqi Housseini , Ahmed Qaddoury","doi":"10.1016/j.aspen.2025.102482","DOIUrl":"10.1016/j.aspen.2025.102482","url":null,"abstract":"<div><div><em>Tylenchulus semipenetrans</em> is a highly damaging obligate plant parasitic nematode (PPN) that poses a significant threat to citrus crops. The use of chemical nematicides has proven effective in reducing <em>T. semipenetrans</em> populations; however, repeated use can have negative environmental impacts, necessitating the adoption of more ecological strategies. The current study aimed to evaluate the nematicidal effect of three aromatic and medicinal plants (AMPs), <em>Rosmarinus officinalis</em>, <em>Laurus nobilis</em> and <em>Verbena officinalis</em> against <em>T. semipenetrans</em> in terms of juvenile J2 mortality and eggs hatch inhibition under greenhouse and laboratory conditions. The results showed that all AMPs aqueous extracts exhibited toxicity against <em>T. semipenetrans</em>, with J2 mortality ranging between 25.85 % and 75.17 %, compared to 1.0 to 13.6 % in the control treatment. Egg hatching varied between 0 % and 66 %, compared to 73 to 95 % in control treatment. Toxicity increased with increasing AMPs aqueous extract concentration and incubation period, reaching more than 55.44 % mortality after 96 h and complete egg hatching inhibition after 144 h of incubation at an <em>R. officinalis</em> aqueous extract concentration of 10 %. In the greenhouse experiment, the aqueous extract of <em>R. officinalis</em> was highly active against <em>T. semipenetrans,</em> resulting in a substantial reduction in nematode populations, with counts ranging from 475 nematodes/100 g of soil and 122 nematodes/10 g of roots, compared to populations of 997.30 nematodes/100 g of soil and 185 nematodes/10 g of roots in the control treatment. The results of this study suggest that AMPs studied, could be used as a bionematicide product for managing <em>T. semipenetrans</em>.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102482"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145097368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insecticidal and antibacterial properties of zinc oxide nanoparticles (ZnO-NPs) synthesized from Phoebe zhennan leaf extract 竹叶提取物合成氧化锌纳米粒子的杀虫抑菌性能

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-12-01 Epub Date: 2025-09-08 DOI: 10.1016/j.aspen.2025.102474

Arshad Ali , Kirshan Chand , Chenghu Liu , Asfandyar , Ali Hassan , Hammad Hassan , Xiaolong Liu , Min Lu

Chemical insecticides are used to control most insect pests, which are harmful to the environment and humans. In this study, green synthesis of zinc oxide nanoparticles (ZnO-NPs) was carried out by using extracts from the Phoebe zhennan plant leaf. X-ray diffraction, transmission electron microscopy (TEM), scanning electron microscopy (SEM), ultraviolet–visible spectroscopy, and Fourier transform infrared spectroscopy (FTIR) confirmed the successful synthesis and structural characteristics of nanoparticles. The forest insect pest Plagiodera versicolora Laicharting is used to evaluate the bioactivity of nanoparticles. Mortality in P. versicolora was found to be dose- and time-dependent after different treatments of nanoparticles. The study determined the lethal concentration values (LC₅₀ and LC₉₀) of nanoparticles against P. versicolora, revealing a time-dependent efficacy with the LC₅₀ of 22.47 mg/L and LC₉₀ of 81.2 mg/L at 96 h. The findings revealed the lethal time values (LT₅₀ and LT₉₀) of the nanoparticles against P. versicolora, with maximum efficacy observed at a concentration of 50 mg/L, resulting in an LT₅₀ of 27.24 h and an LT₉₀ of 62.71 h. In addition, the antibacterial activity of nanoparticles was also evaluated against Bacillus subtilis, and the results showed significant inhibition of bacterial growth in a dose-dependent manner. The study demonstrated the potential of nanoparticles as an insect control and antimicrobial agent. This research provides valuable insights into the green synthesis of nanoparticles and emphasizes the need for further exploration of their safety and efficacy in various practical applications.

化学杀虫剂用于控制大多数对环境和人类有害的害虫。本研究以真南叶提取物为原料，进行了氧化锌纳米颗粒（ZnO-NPs）的绿色合成。x射线衍射、透射电子显微镜（TEM）、扫描电子显微镜（SEM）、紫外可见光谱和傅里叶变换红外光谱（FTIR）证实了纳米颗粒的成功合成和结构特征。利用森林害虫花斑曲蝗（Plagiodera versicolora Laicharting）对纳米颗粒的生物活性进行了评价。在不同的纳米颗粒处理后，发现斑孢霉的死亡率与剂量和时间有关。实验测定了纳米颗粒对紫斑拟虫的致死浓度（LC50和LC90）， 96 h时LC50和LC90分别为22.47 mg/L和81.2 mg/L，具有时间依赖性。结果表明，纳米颗粒对紫斑拟虫的致死时间（LT50和LT90）在浓度为50 mg/L时达到最大，LT50和LT90分别为27.24 h和62.71 h。纳米颗粒对枯草芽孢杆菌的抑菌活性也进行了评估，结果表明纳米颗粒对枯草芽孢杆菌的生长有明显的抑制作用，且呈剂量依赖性。该研究证明了纳米颗粒作为昆虫控制和抗菌剂的潜力。本研究为纳米颗粒的绿色合成提供了有价值的见解，并强调了在各种实际应用中进一步探索其安全性和有效性的必要性。

{"title":"Insecticidal and antibacterial properties of zinc oxide nanoparticles (ZnO-NPs) synthesized from Phoebe zhennan leaf extract","authors":"Arshad Ali , Kirshan Chand , Chenghu Liu , Asfandyar , Ali Hassan , Hammad Hassan , Xiaolong Liu , Min Lu","doi":"10.1016/j.aspen.2025.102474","DOIUrl":"10.1016/j.aspen.2025.102474","url":null,"abstract":"<div><div>Chemical insecticides are used to control most insect pests, which are harmful to the environment and humans. In this study, green synthesis of zinc oxide nanoparticles (ZnO-NPs) was carried out by using extracts from the <!-->antibacterial activity of nanoparticles was also evaluated against <em>Bacillus subtilis</em>, and the results showed significant inhibition of bacterial growth in a dose-dependent manner. The study demonstrated the potential of nanoparticles as an insect control and antimicrobial agent. This research provides valuable insights into the green synthesis of nanoparticles and emphasizes the need for further exploration of their safety and efficacy in various practical applications.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102474"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Presence of a queen (Apis mellifera) reduces the sensitivity of worker bees to environmental risks 蜂王（蜜蜂）的存在降低了工蜂对环境风险的敏感性

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-12-01 Epub Date: 2025-09-27 DOI: 10.1016/j.aspen.2025.102485

De Fang Niu , Qiu Lan Zheng , Bin Yuan , Xiao Ling Su , Cui Ping Zhang , Yuan Yuan Lu , Ping Liu , Fu Liang Hu , Huo Qing Zheng

In honeybees (Apis mellifera), the queen is the central node of the colony; however, the mechanisms through which her presence mitigates the colony’s collective vulnerability to environmental stressors are not well understood. This study investigates the importance of within-colony interactions, particularly the interactions between the queen and worker bees, on colony development under environmental stress. Compared to worker bees in queenless colonies, the presence of a queen resulted in higher survival rates and food intake when exposed to 450 μg/mL of tetracycline. The queen also modulated antioxidant responses, particularly enhancing superoxide dismutase (SOD) activity. Transcriptome analysis indicated that the presence of a queen downregulated the expression of genes involved in the Toll-like receptor (Toll) and Immune deficiency (Imd) pathways, ascorbate and aldarate metabolism, and retinol metabolism in individual worker bees. These results indicate that interactions between queen and worker bees strengthen their immunity and increase the response threshold to environmental stress. Therefore, individual interactions should be included in environmental risk assessments, especially for superorganisms such as honeybees.

在蜜蜂（Apis mellifera）中，蜂王是群体的中心节点；然而，她的存在减轻了群体对环境压力的集体脆弱性的机制尚不清楚。本研究探讨了环境压力下蜂群内部相互作用，特别是蜂王与工蜂之间的相互作用对蜂群发展的重要性。与无蜂王的工蜂相比，当暴露于450 μg/mL的四环素中时，蜂王的存在导致了更高的存活率和食物摄入量。女王还可以调节抗氧化反应，特别是提高超氧化物歧化酶（SOD）的活性。转录组分析表明，蜂王的存在下调了工蜂个体中Toll样受体（Toll）和免疫缺陷（Imd）途径、抗坏血酸和醛酸盐代谢以及视黄醇代谢相关基因的表达。这些结果表明，蜂王与工蜂的相互作用增强了蜂王的免疫力，提高了蜂王对环境压力的反应阈值。因此，个体的相互作用应包括在环境风险评估中，特别是对于像蜜蜂这样的超级有机体。

{"title":"Presence of a queen (Apis mellifera) reduces the sensitivity of worker bees to environmental risks","authors":"De Fang Niu , Qiu Lan Zheng , Bin Yuan , Xiao Ling Su , Cui Ping Zhang , Yuan Yuan Lu , Ping Liu , Fu Liang Hu , Huo Qing Zheng","doi":"10.1016/j.aspen.2025.102485","DOIUrl":"10.1016/j.aspen.2025.102485","url":null,"abstract":"<div><div>In honeybees (<em>Apis mellifera</em>), the queen is the central node of the colony; however, the mechanisms through which her presence mitigates the colony’s collective vulnerability to environmental stressors are not well understood. This study investigates the importance of within-colony interactions, particularly the interactions between the queen and worker bees, on colony development under environmental stress. Compared to worker bees in queenless colonies, the presence of a queen resulted in higher survival rates and food intake when exposed to 450 μg/mL of tetracycline. The queen also modulated antioxidant responses, particularly enhancing superoxide dismutase (SOD) activity. Transcriptome analysis indicated that the presence of a queen downregulated the expression of genes involved in the Toll-like receptor (Toll) and Immune deficiency (Imd) pathways, ascorbate and aldarate metabolism, and retinol metabolism in individual worker bees. These results indicate that interactions between queen and worker bees strengthen their immunity and increase the response threshold to environmental stress. Therefore, individual interactions should be included in environmental risk assessments, especially for superorganisms such as honeybees.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102485"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145358744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0