Journal of Asia-pacific Entomology最新文献_第2页

Identification and stress-responsive expression analysis of two Hsp70 genes in Epicauta impressicornis under temperature and diapause conditions 温滞育条件下印角壁鱼Hsp70基因的鉴定及应激响应分析

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2026-01-16 DOI: 10.1016/j.aspen.2026.102521

Qing Yang , Yangyang Liu , Xuyu Ran , Ling Yang , Jiankun Long , Zhimin Chang , Xiangsheng Chen

The blister beetle Epicauta impressicornis is known for producing cantharidin, a compound that exhibits anticancer properties. Heat shock protein 70 (Hsp70) plays a vital role in helping insect adapt to stress. The aim of this study was to clone and characterise two Hsp70 genes (EiHsp70-1 and EiHsp70-2) from this beetle, and to investigate how they are expressed in response to temperature variations and diapause. The full-length cDNA sequences of EiHsp70-1 and EiHsp70-2 were cloned and bioinformatically analysed using available transcriptomic data (PRJNA679947). Two cytosolic Hsp70 genes were successfully identified, each of which contained conserved signature motifs and a C-terminal EEVD sequence. Phylogenetic analysis revealed a high degree of homology with Hsp70s from other insects, particularly E. impressicornis, emphasising evolutionary conservation. The two Hsp70 genes in E. impressicornis exhibited the lowest levela of expression on day 3 of the fifth instar, with the highest levels observed in thoracic tissue and during diapause. Expression was strongly up-regulated under high-temperature stress (36 °C), followed by low-temperature stress (12 °C), and was lowest at 30 °C. The expression patterns observed for EiHsp70-1 and EiHsp70-2 suggest their potential roles in enhancing thermal tolerance and modulating diapause-related stress responses in E. impressicornis. This research lays the groundword for understanding of Hsp70 function in insect stress adaptation, particularly during overwintering under extreme temperatures.

水疱甲虫以生产斑蝥素而闻名，斑蝥素是一种具有抗癌特性的化合物。热休克蛋白70 （Hsp70）在帮助昆虫适应应激中起着至关重要的作用。本研究的目的是克隆和表征该甲虫的两个Hsp70基因（EiHsp70-1和EiHsp70-2），并研究它们在温度变化和滞育中的表达方式。克隆了EiHsp70-1和EiHsp70-2的全长cDNA序列，并利用现有转录组学数据（PRJNA679947）进行生物信息学分析。成功鉴定了两个胞质Hsp70基因，每个基因都包含保守的特征基序和c端EEVD序列。系统发育分析显示，该基因与其他昆虫的hsp70具有高度的同源性，特别是与印染伊蚊的hsp70具有高度的同源性。这两个Hsp70基因在5龄第3天表达量最低，在胸组织和滞育期间表达量最高。高温胁迫（36℃）下表达量显著上调，低温胁迫（12℃）次之，30℃时表达量最低。EiHsp70-1和EiHsp70-2的表达模式表明它们可能在增强耐温性和调节滞育相关的胁迫反应中发挥作用。本研究为了解Hsp70在昆虫逆境适应中的作用，特别是在极端温度下越冬的作用奠定了基础。

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引用次数: 0

Temporal dynamics of insecticide-resistance mutations in the diamondback moth Plutella xylostella (Linnaeus, 1758) within northern immigration regions 北方移民区小菜蛾（Plutella xylostella, Linnaeus, 1758）杀虫剂抗性突变的时间动态

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2026-02-09 DOI: 10.1016/j.aspen.2026.102532

Ya-Jun Qu , Jin-Cui Chen , Hui-Juan Li , Shuai-Yu Wang , Xue-Yan Zhang , Ary A. Hoffmann , Sen-Shan Wang , Shu-Jun Wei

The diamondback moth (DBM), Plutella xylostella (Linnaeus, 1758) (Lepidoptera: Plutellidae), is a migratory pest of cruciferous crops. This species cannot survive winters in high-latitude regions, where populations primarily consist of migrants from year-round breeding areas. The movement of these moths between regions can influence insecticide resistance patterns in the recipient region by introducing resistant alleles from source populations. In this study, we investigated the adult population dynamics of DBM in two recipient locations in northern China over multiple years and analyzed variation in resistance mutations during this period. The two sampling locations showed similar DBM population dynamics, with the three highest peaks in the number of captured DBM occurring in late May, late June, and mid-August. Among the 16 loci, Q4594L showed the highest mean frequency, followed by A1060T and G324A. Although average frequencies of resistance mutations remained relatively stable across years, there were marked fluctuations in May to June and August to September, which may be related to the introduction of varied sources by migration and/or local selection. Additionally, we found that deviations from Hardy-Weinberg equilibrium were common for many of the resistance loci. Our study demonstrated variation in resistance mutation frequency in the DBM, likely caused by immigrants from various sources and local selection. These results provide novel insights into the temporal dynamics of insecticide resistance in DBM. They may help in targeting chemical control of this pest, but additional data are required to understand the impacts of long-distance migration versus local selection on resistance.

小菜蛾（Plutella xylostella, Linnaeus, 1758）（鳞翅目：小菜蛾科）是十字花科作物的一种迁徙害虫。这个物种不能在高纬度地区过冬，那里的种群主要是由全年繁殖地区的迁徙者组成的。这些飞蛾在区域之间的移动可以通过从源种群引入抗性等位基因来影响接收区域的杀虫剂抗性模式。在这项研究中，我们调查了中国北方两个受体地区多年来DBM的成年种群动态，并分析了这一时期的抗性突变变化。2个采样点DBM种群动态相似，捕获DBM数量的3个高峰出现在5月下旬、6月下旬和8月中旬。16个基因座中，Q4594L的平均频率最高，其次是A1060T和G324A。尽管耐药突变的平均频率多年来保持相对稳定，但5月至6月和8月至9月出现明显波动，这可能与通过迁徙和/或当地选择引入各种来源有关。此外，我们发现许多抗性位点偏离Hardy-Weinberg平衡是常见的。我们的研究表明，DBM中抗性突变频率的变化可能是由来自不同来源的移民和当地选择引起的。这些结果为DBM杀虫剂抗性的时间动态提供了新的见解。它们可能有助于对这种害虫进行针对性的化学控制，但需要更多的数据来了解远距离迁移与本地选择对抗性的影响。

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引用次数: 0

The bumblebee Niemann–Pick type C2 protein binds to sperm and is upregulated in the antennae under thermal stress 大黄蜂Niemann-Pick型C2蛋白与精子结合，并在热应激下在触角中上调

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2026-02-08 DOI: 10.1016/j.aspen.2026.102531

Bo Yeon Kim , Jin Myung Kim , Hyung Joo Yoon , Kwang Sik Lee , Byung Rae Jin

Niemann–Pick type C2 protein (NPC2) in insects is involved in various physiological processes, including lipid metabolism, immune response, chemical communication, and sperm viability. However, the expression profile of NPC2 under thermal stress remains unclear. Thus, this study aimed to investigate the binding of the bumblebee Bombus terrestris NPC2 (BtNPC2) protein to sperm, as well as the BtNPC2 gene expression patterns in male B. terrestris tissues under thermal stress. Binding assays were performed using recombinant BtNPC2, and the specific BtNPC2 antibody demonstrated that BtNPC2 binds to the sperm surface; thus, BtNPC2 functions as a sperm-binding protein in the seminal fluids of males. Furthermore, BtNPC2 was highly expressed in the testes and antennae of B. terrestris males, with a significant upregulation in expression noted in the antennae under high-temperature stress. These findings suggest that BtNPC2 may play a role in the physiological responses conducted in the antennae of bumblebees under thermal stress. Our results support the multifunctional roles of NPC2 in bumblebees.

昆虫体内的Niemann-Pick型C2蛋白（NPC2）参与多种生理过程，包括脂质代谢、免疫反应、化学通讯和精子存活等。然而，NPC2在热应力下的表达谱尚不清楚。因此，本研究旨在研究热胁迫下雄性地熊蜂（bumblebee Bombus terrestris） NPC2 （BtNPC2）蛋白与精子的结合，以及BtNPC2基因在雄性地熊蜂组织中的表达模式。结合实验采用重组BtNPC2进行，特异性BtNPC2抗体表明BtNPC2与精子表面结合；因此，BtNPC2在雄性精液中作为精子结合蛋白发挥作用。此外，BtNPC2在雄性地夜蛾睾丸和触角中高表达，且在高温胁迫下触角中的表达显著上调。这些发现表明BtNPC2可能在热应激下大黄蜂触角的生理反应中发挥作用。我们的研究结果支持NPC2在大黄蜂中的多功能作用。

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引用次数: 0

Body size- and water homeostasis-dependent temperature stress tolerance and transcriptional changes in red fire ant workers 红火蚁工蚁体型和水分稳态依赖的温度胁迫耐受性和转录变化

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2025-12-17 DOI: 10.1016/j.aspen.2025.102518

Wei Gao , Rui Peng , Jian-min Yang , Jian-lin Lu , Hong-yun Cao , Ying-huan Li , Chun Li , Wen Fu , Jin Xu

The physiological processes that link body size to environmental temperature and humidity is vital for understanding the evolutionary significance of insect adaptation strategies and mechanisms. In this study, we demonstrated that both cold and heat stresses significantly affected the survival of Solenopsis invicta workers, with smaller worker ants showing higher mortality than larger ones. Workers who were provided with water or sucrose solution exhibited significantly higher survival under temperature stress than controls. Further measurements showed that smaller workers usually had a higher water loss rate than larger ones, especially under desiccation conditions. Body size- and temperature-specific transcriptional changes were found, where smaller workers had more differentially expressed genes (DEGs) than larger ones, suggesting that smaller workers are likely to incur greater temperature stresses. The most prominent changes were the remarkable upregulation of Hsps related genes in heat stressed workers, whereas their changes in cold stressed workers were not significant. Both cold and heat stresses induced significantly changes in small molecule protectants, antioxidants, cuticle and excretory related DEGs and pathways. Further, a Vasopressin-regulated water reabsorption pathway was enriched in both cold and heat stressed workers. Further analysis revealed 22 water balance related DEGs, such as neurophysin, capa receptor, and diuretic hormone. These results indicate that water homeostasis is crucial for the survival of ants under extreme hot and cold conditions.

研究昆虫体型与环境温度和湿度之间的生理过程，对于理解昆虫适应策略和机制的进化意义至关重要。在这项研究中，我们证明了冷应激和热应激都显著影响了无头螺梭工蚁的生存，小工蚁的死亡率高于大工蚁。提供水或蔗糖溶液的工人在温度胁迫下的存活率明显高于对照组。进一步的测量表明，体型较小的工人通常比体型较大的工人失水率更高，特别是在干燥条件下。研究发现，体型较小的工蜂比体型较大的工蜂有更多的差异表达基因（deg），这表明体型较小的工蜂可能会承受更大的温度压力。热应激工人的热休克蛋白相关基因表达显著上调，而冷应激工人的热休克蛋白相关基因表达变化不显著。冷应激和热应激均引起小分子保护剂、抗氧化剂、角质层和排泄相关deg和途径的显著变化。此外，抗利尿激素调节的水重吸收途径在冷应激和热应激工人中都丰富。进一步分析发现22种与水平衡相关的deg，如神经生理素、capa受体和利尿激素。这些结果表明，水分平衡对于蚂蚁在极端炎热和寒冷条件下的生存至关重要。

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引用次数: 0

A C-type lectin from domestic silkworm (Bombyx mori) is involved in bacterial recognition and participates in prophenoloxidase activation 家蚕c型凝集素参与细菌识别并参与酚氧化酶原活化

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2026-01-09 DOI: 10.1016/j.aspen.2026.102522

Yan Huang , Jing Zhang , Jingyao Shi , Jie Wu , Dan Zhang , Qiaoling Zhao , Dongxu Shen

C-type lectins (CTLs), which belong to the superfamily of glycan-binding proteins, are characterized by the presence of one or more carbohydrate recognition domains (CRDs). CTLs are involved in biological processes, such as immune recognition, intercellular signaling, cell adhesion, and pathogen clearance, and are widely distributed across diverse organisms, from invertebrates to vertebrates. In this study, we investigated the function of the C-type lectin gene named BmIML-2, in the innate immune response of the domestic silkworm, Bombyx mori. First, our results suggested that BmIML-2 was highly expressed in fat body and its transcript levels were significantly up-regulated after induction by different species of bacteria. Afterwards, BmIML-2 was recombinantly expressed using a prokaryotic expression system to delve into its physiological function. Binding assays showed that recombinant BmIML-2 (rBmIML-2) exhibits binding capacity to bacteria, including Escherichia coli, Micrococcus luteus, Staphylococcus aureus and Bacillus subtilis. In addition, Enzyme linked immunosorbent assay (ELISA) demonstrated that rBmIML-2 also binds to cell wall components, such as lipopolysaccharide (LPS) and peptidoglycan (PGN), with more intensely binding affinity to the former. Furthermore, encapsulation assays in vitro suggested that rBmIML-2 was able to accelerate encapsulation and melanization mediated by hemocytes. Finally, we demonstrated that rBmIML-2 significantly potentiates the stimulation pathway of prophenoloxidase (PPO) in plasma. In summary, our results demonstrate that BmIML-2 can act as a pattern recognition receptor (PRR) involved in the host innate immune response.

c型凝集素（ctl）属于聚糖结合蛋白超家族，其特征是存在一个或多个碳水化合物识别结构域（CRDs）。ctl参与生物过程，如免疫识别、细胞间信号传导、细胞粘附和病原体清除，广泛分布于从无脊椎动物到脊椎动物的各种生物中。在这项研究中，我们研究了c型凝集素基因BmIML-2在家蚕先天免疫应答中的功能。首先，我们的研究结果表明，BmIML-2在脂肪体中高表达，在不同种类细菌的诱导下，其转录水平显著上调。随后，利用原核表达系统重组表达bmml -2，深入研究其生理功能。结合实验表明，重组BmIML-2 （rBmIML-2）具有与大肠杆菌、黄体微球菌、金黄色葡萄球菌和枯草芽孢杆菌等细菌的结合能力。此外，酶联免疫吸附试验（ELISA）表明rBmIML-2还与细胞壁成分如脂多糖（LPS）和肽聚糖（PGN）结合，且前者的结合亲和力更强。此外，体外包封实验表明，rBmIML-2能够加速血细胞介导的包封和黑色素化。最后，我们证明rBmIML-2显著增强了血浆中酚氧化酶原（PPO）的刺激途径。综上所述，我们的研究结果表明bmml -2可以作为一种模式识别受体（PRR）参与宿主先天免疫反应。

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引用次数: 0

Exploring the relationship between dung beetles and mammalian species in Indian subcontinent 探索印度次大陆的蜣螂与哺乳动物物种之间的关系

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2026-02-24 DOI: 10.1016/j.aspen.2026.102538

Sourabh Verma , Neeraj Narayan , Piyush Barnwal , Nesamani Kandasamy , Bhupendra Kumar

Dung beetles are opportunistic feeders that consume various types of mammalian dung. In our study at Valmiki Tiger Reserve, India, we collected 5,645 individual dung beetles from two sites. At Site 1 (Sal Forest with Phoenix), we found 2,680 beetles across 45 species, while at Site 2 (Mixed moist Deciduous Forest), we recorded 965 beetles from 30 species. The highest species richness at Site 1 was in spotted deer dung, while at Site 2, it was in human feces; sambar dung had the lowest species richness at both sites. Sisyphus longipes was the most abundant dung beetle species at Site 1, and Onthophagus hamaticeps at Site 2. Resource partitioning among dung beetle species was generally low, indicating low food specialization. However, some species, like Paragymnopleurus sinuatus and Onthophagus crassicollis at Site 1, exhibited high dung specialization, while Copris sinicus and Sisyphus hirtus showed more generalized feeding patterns. At Site 2, Onthophagus hamaticeps and Onthophagus crassicollis displayed high dung specialization, whereas Caccobius unicornis and Onthophagus abreui were more generalized. This study suggests that the dung preference of dung beetles is species specific, and varies with both the mammalian species and the feeding site.

屎壳郎是机会主义食性动物，吃各种哺乳动物的粪便。在我们在印度瓦尔米基老虎保护区的研究中，我们从两个地点收集了5645只蜣螂。在样地1（有凤凰的萨尔林）共发现45种2680只甲虫，在样地2（潮湿落叶混交林）共发现30种965只甲虫。站点1中物种丰富度最高的是鹿粪，站点2中物种丰富度最高的是人粪；两个样点的物种丰富度均最低。在第1点，长角西西弗斯（Sisyphus longipes）是最丰富的屎壳郎种类，在第2点，是hamaticeps Onthophagus。屎壳郎种间资源分配普遍较低，食物专门化程度较低。然而，一些物种，如Paragymnopleurus sinuatus和Onthophagus crassicollis在站点1表现出高度的粪便专业化，而Copris sinicus和Sisyphus hirtus则表现出更普遍的取食模式。在站点2，食腐菌hamaticeps和Onthophagus crassicollis表现出高度的粪便专门化，而Caccobius unicornis和Onthophagus abreui表现出更普遍的粪便专门化。研究表明，屎壳郎的粪偏好具有物种特异性，且随哺乳动物种类和取食地点的不同而不同。

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引用次数: 0

Thermal and host-plant modulation of development, reproduction, and population growth of the spiraling whitefly, Aleurodicus dispersus (Hemiptera: Aleyrodidae) 粉虱发育、繁殖和种群增长的热调控和寄主植物调控（半翅目：粉虱科）

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2026-02-19 DOI: 10.1016/j.aspen.2026.102534

T. Boopathi , N. Anusha , J.G. Prasuna , K. Divya

Temperature and host-plant quality are key ecological drivers influencing the physiology, reproduction, and population dynamics of poikilothermic insect pests by directly constraining metabolic rates and energy allocation typical of ectotherms. This study investigated the interactive effects of temperature (25, 30, and 35 °C) and host plants (cassava and eggplant) on the developmental duration, fecundity, longevity, and demographic parameters of the spiraling whitefly, Aleurodicus dispersus (Hemiptera: Aleyrodidae), under controlled conditions. Developmental rate increased with temperature within the tested range up to 30 °C, followed by a sharp decline at 35 °C, indicating thermal stress under the highest temperature treatment. Cassava consistently supported shorter developmental periods, higher fecundity, and greater intrinsic (r = 0.066 day⁻¹) and net reproductive rates (R₀ = 9.94 offspring female⁻¹) compared with eggplant. Regression analyses revealed overall negative associations between total developmental period and fecundity, and positive association between egg hatchability and adult emergence, largely reflecting temperature-driven differences among treatments. Age-stage, two-sex life table analysis indicated 30 °C as the most favorable temperature among those tested for population growth, while performance was substantially reduced at 35 °C. These findings highlight that temperature and host quality jointly shape the demographic potential of A. dispersus, offering valuable insights for forecasting pest outbreaks and developing climate-informed integrated pest management (IPM) strategies in tropical and subtropical cropping systems.

温度和寄主植物质量是影响变温昆虫生理、繁殖和种群动态的关键生态驱动因素，直接制约着变温动物典型的代谢率和能量分配。研究了温度（25℃、30℃和35℃）和寄主植物（木薯和茄子）在控制条件下对螺旋粉虱（半翅目：粉虱科）发育持续时间、繁殖力、寿命和种群参数的交互影响。在测试温度范围内，发育速率随温度升高而升高，直至35℃，发育速率急剧下降，表明在最高温度处理下存在热应力。与茄子相比，木薯始终具有更短的发育周期，更高的繁殖力，更高的内在繁殖率（r = 0.066天−1）和净繁殖率（R0 = 9.94雌性后代−1）。回归分析显示，总发育期与繁殖力总体呈负相关，而卵孵化率与成虫羽化呈正相关，这在很大程度上反映了不同处理之间温度驱动的差异。年龄阶段，两性生命表分析表明，30°C是最有利于种群生长的温度，而35°C则大大降低了种群的性能。这些发现突出表明，温度和寄主质量共同塑造了散弹弹的种群潜力，为预测热带和亚热带种植系统的病虫害暴发和制定气候知情的病虫害综合治理（IPM）战略提供了有价值的见解。

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引用次数: 0

Removing gut commensal bacteria affects nutritional performance of diapause-destined larvae of Helicoverpa armigera 去除肠道共生菌会影响棉铃虫滞育幼虫的营养性能

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2026-01-03 DOI: 10.1016/j.aspen.2025.102517

Hoda Zamani, Azam Mikani, Mohammad Mehrabadi, Saeid Moharramipour

Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) is a highly polyphagous species that undergoes pupal diapause when fifth-instar larvae are exposed to short day lengths, enabling survival under unfavorable conditions. Diapause-destined larvae accumulate nutritional reserves that sustain the pupal stage, yet the role of gut symbiotic bacteria in this process remains unclear. We eliminated gut microbiota with antibiotics and compared nutritional indices, approximate digestibility (AD), efficiency of conversion of ingested food (ECI), efficiency of conversion of digested food (ECD), relative consumption rate (RCR), and relative growth rate (RGR), in diapause-destined (D) and nondiapause (ND) larvae during the fifth and sixth instars. Diapause-destined larvae with intact gut microbiota (D⁺) exhibited higher ECI, ECD, and RGR but lower RCR than antibiotic-treated larvae (D⁻), indicating efficient nutrient conversion and energy accumulation. In contrast, D⁻ larvae consumed more food but achieved lower growth efficiency, suggesting compensatory feeding. Similar trends were observed in nondiapause larvae, with ND⁺ individuals displaying higher growth efficiency than ND⁻ counterparts. Nutritional indices were generally greater in sixth instar larvae compared to fifth instar larvae, reflecting intensified reserve accumulation during the prediapause phase. Removal of gut bacteria reduced larval weight, fecal output, and conversion efficiencies, highlighting their importance in nutritional performance and biomass gain. These findings demonstrate that gut commensal bacteria play a critical role in optimizing nutrient utilization and supporting diapause preparation in H. armigera.

棉蚜Helicoverpa armigera (h bner)（鳞翅目：夜蛾科）是一种高度多食性的物种，当五龄幼虫暴露于较短的日照时，蛹会发生滞育，从而使其能够在不利的条件下生存。滞育幼虫积累营养储备以维持蛹期，但肠道共生细菌在这一过程中的作用尚不清楚。用抗生素消除肠道菌群，比较了滞育期(D)和非滞育期（ND）幼虫在5龄和6龄期间的营养指标：近似消化率（AD）、摄入食物转化效率（ECI）、消化食物转化效率（ECD）、相对消耗率（RCR）和相对生长率（RGR）。肠道菌群完整的滞育幼虫（D+）表现出更高的ECI、ECD和RGR，但RCR低于抗生素处理的幼虫（D -），表明营养转化和能量积累效率更高。相比之下，D−幼虫消耗更多的食物，但生长效率较低，这可能是代偿性摄食。在非滞育幼虫中也观察到类似的趋势，ND+个体的生长效率高于ND -个体。6龄幼虫的营养指标普遍高于5龄幼虫，反映了滞育前期储备积累的加剧。肠道细菌的去除降低了幼虫的体重、粪便排出量和转化效率，突出了它们在营养性能和生物量增加方面的重要性。这些结果表明，肠道共生菌在优化棉铃虫营养利用和支持滞育准备方面起着关键作用。

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引用次数: 0

Diverse cellulolytic bacterial community in the gut of Trypoxylus dichotomus larvae: a promising source for novel cellulose-degrading microorganisms 二叉地瓜幼虫肠道内纤维素分解菌群的多样性：新型纤维素降解微生物的一个有希望的来源

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2026-01-26 DOI: 10.1016/j.aspen.2026.102525

Ao Li , Linyao Weng , Jiajing Ye, Simin Peng, Yujie Fan, Xinqi Zhang, Qingyun Wang, Shouke Zhang, Junhao Huang

The gut microbiota of herbivorous insects is crucial for cellulose degradation. This study characterized the cellulolytic bacterial community in Trypoxylus dichotomus larvae, isolating 115 aerobic and facultatively anaerobic strains. 16S rDNA sequence analysis classified these isolates into 30 species, 19 genera, 13 families, and 4 phyla, with Pseudomonadota dominating (71.0%), followed by Actinobacteria (12.9%), Firmicutes (9.7%), and Bacteroidetes (6.4%). Distinct compositional differences were observed between the midgut and hindgut communities. Notably, the cellulolytic diversity in T. dichotomus is comparable to that of termites, indicating convergent, specialized adaptation for lignocellulose digestion. These findings establish the rhinoceros beetle gut as a significant reservoir for cellulolytic microbes and provide insights into the evolutionary adaptations of detritivorous insects and their symbiotic microbiota for efficient lignocellulose degradation.

食草昆虫的肠道微生物群对纤维素的降解至关重要。本研究通过分离出115株好氧和兼性厌氧菌株，对二叉地沟霉幼虫的纤维素降解菌群进行了研究。16S rDNA序列分析结果显示，这些分离物隶属于4门13科19属30种，其中假单胞菌门（Pseudomonadota）占71.0%，放线菌门（Actinobacteria）占12.9%，厚壁菌门（Firmicutes）占9.7%，拟杆菌门（Bacteroidetes）占6.4%。在中肠和后肠群落中观察到明显的组成差异。值得注意的是，T. dichotomus的纤维素分解多样性与白蚁相当，表明对木质纤维素消化的趋同，专门的适应。这些发现证实了犀牛甲虫肠道是纤维素分解微生物的重要储存库，并为了解食腐昆虫及其共生微生物群对有效降解木质纤维素的进化适应性提供了见解。

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引用次数: 0

Temperature-dependent fecundity of Delia platura (Meigen) (Diptera: Anthomyiidae) and life table parameters in the laboratory 双翅目：花蝇科斑蝶的温度依赖性繁殖力及实验室生命表参数

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2026-03-01 Epub Date: 2026-02-06 DOI: 10.1016/j.aspen.2026.102529

Han Ni Aye , Hyeon Suk Jo , Dong-Soon Kim

Understanding the thermal sensitivity of Delia platura (Meigen) (Diptera: Anthomyiidae), a widespread polyphagous pest, is crucial for predicting its population dynamics and improving pest management strategies. We investigated the temperature-dependent reproductive and developmental traits of D. platura under five constant temperature regimes (15, 19, 23, 27, and 30°C). Adults showed relatively long lifespans with considerable inter-individual variation, contributing to the observed variability in oviposition schedules-particularly at 23°C and 27°C, where fecundity patterns were highly dispersed and multimodal. Fecundity peaked at 27°C (98 eggs), while oviposition at 30°C declined rapidly and no pupae survived to adulthood, indicating a marginal upper limit for population persistence. Life table parameters showed clear temperature dependence. The intrinsic rate of increase (r_m) remained low at cooler temperatures (0.019 at 15°C and 0.020 at 19°C), but increased substantially at warmer temperatures, reaching 0.033 at 23°C and peaking at 0.039 at 27°C. At 30°C, no viable development was observed, preventing life table estimation. Our findings provide the first integrative dataset for temperature-dependent fecundity and development of D. platura and support its application in mechanistic modeling for seasonal forecasting and climate risk assessment.

摘要花蝇科（diia platura, Meigen，双翅目：花蝇科）是一种广泛分布的多食害虫，了解其热敏性对预测其种群动态和改进害虫防治策略具有重要意义。研究了五种恒温环境（15、19、23、27和30°C）下的斑胸草（D. platura）的生殖和发育性状。成虫的寿命相对较长，但个体间差异很大，这导致了观察到的产卵时间表的差异——特别是在23°C和27°C时，繁殖力模式高度分散和多模式。27°C时繁殖力达到顶峰（98个卵），而30°C时产卵量迅速下降，没有蛹存活到成虫，这表明种群持续存在的边际上限。寿命表参数表现出明显的温度依赖性。在较低的温度下（15°C时为0.019,19°C时为0.020），内在增长率（rm）仍然很低，但在较高的温度下显著增加，在23°C时达到0.033，在27°C时达到0.039的峰值。在30°C时，没有观察到有活力的发育，妨碍了生命表的估计。我们的研究结果提供了第一个基于温度依赖性的白杨繁殖力和发育的综合数据集，并支持其在季节预报和气候风险评估机制建模中的应用。

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引用次数: 0