Pub Date : 2023-07-26DOI: 10.24126/jobrc.2023.17.2.684
Mohammed Khafaji, Alaa Taha
Conclusion: Light significantly affected the concentration of several secondary metabolism chemicals. Despite this, UV light showed that the majority of secondary metabolites of N. niger had high significance when exposed to UV light for 120 min. This included a rise in levels of all compounds except for atropine, which showed a significant increase after only 60 minutes of UV exposure.
{"title":"Photoperiod and UV light influence secondary metabolites of Hyoscyamus niger callus induced from leaves","authors":"Mohammed Khafaji, Alaa Taha","doi":"10.24126/jobrc.2023.17.2.684","DOIUrl":"https://doi.org/10.24126/jobrc.2023.17.2.684","url":null,"abstract":"Conclusion: Light significantly affected the concentration of several secondary metabolism chemicals. Despite this, UV light showed that the majority of secondary metabolites of N. niger had high significance when exposed to UV light for 120 min. This included a rise in levels of all compounds except for atropine, which showed a significant increase after only 60 minutes of UV exposure.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139354860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-26DOI: 10.24126/jobrc.2023.17.2.715
Israa K. Hussein, Wafaa S. Shani
Background: Diabetic foot ulcer (DFU) is a common serious complication of diabetes. Nowadays there is a suggestion that referred to the fact the chronicity of DFU is a result of the positive feedback of proinflammatory cytokines production. Objective: This study aimed to display the negative effects of persistent expression of proinflammatory cytokines of (IL-1β and IL-18) under hyperglycemic condition on the DFU microenvironment and clarify the role of growth factor Milk fat globule-epidermal growth factor- 8( MFG-) in the DFU healing process. Approach: Clinical data of a total of 49 type 2 diabetic patients have chronic diabetic foot ulcer grade 2 and 3 (infected DFU n= 29 and non-infected n=21) in addition to the control group (n=25) were participated in the study since June 2021 to April 2022 , venous blood samples were collected for the measurement of the gene expression levels of (IL-1β ; IL-18 and MFG-E8) by Real-Time PCR technique . Results: There was no significant difference in age and sex when compared with the control group at p= 0.798, p =0.514 respectively. High levels of gene expression of (IL-1β ; IL-18 and MFG-E8) were shown in the patients group in comparison to the healthy control. Conclusions: Current data confirmed that the inflammatory mediators’ (IL-1β ; IL-18 and MFG-E8) have a strong association with the pathogenesis of DFU healing process.
{"title":"Gene expression levels of inflammatory mediators in diabetic foot ulcer patients","authors":"Israa K. Hussein, Wafaa S. Shani","doi":"10.24126/jobrc.2023.17.2.715","DOIUrl":"https://doi.org/10.24126/jobrc.2023.17.2.715","url":null,"abstract":"Background: Diabetic foot ulcer (DFU) is a common serious complication of diabetes. Nowadays there is a suggestion that referred to the fact the chronicity of DFU is a result of the positive feedback of proinflammatory cytokines production. Objective: This study aimed to display the negative effects of persistent expression of proinflammatory cytokines of (IL-1β and IL-18) under hyperglycemic condition on the DFU microenvironment and clarify the role of growth factor Milk fat globule-epidermal growth factor- 8( MFG-) in the DFU healing process. Approach: Clinical data of a total of 49 type 2 diabetic patients have chronic diabetic foot ulcer grade 2 and 3 (infected DFU n= 29 and non-infected n=21) in addition to the control group (n=25) were participated in the study since June 2021 to April 2022 , venous blood samples were collected for the measurement of the gene expression levels of (IL-1β ; IL-18 and MFG-E8) by Real-Time PCR technique . Results: There was no significant difference in age and sex when compared with the control group at p= 0.798, p =0.514 respectively. High levels of gene expression of (IL-1β ; IL-18 and MFG-E8) were shown in the patients group in comparison to the healthy control. Conclusions: Current data confirmed that the inflammatory mediators’ (IL-1β ; IL-18 and MFG-E8) have a strong association with the pathogenesis of DFU healing process.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139354452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-26DOI: 10.24126/jobrc.2023.17.2.734
Yasameen A. Hadi, Risalla H. ALlami, Ahmed A. Suleiman
Background :GDM (gestational diabetes mellitus) is a hazardous pregnancy situation that occurs when a pregnant woman who that have never had diabetes progresses to cause systemic hyperglycemia during their pregnancy.DNA methylation is an epigenetic mechanism it occurs in the vertebrate genomes and generates 5-methylcytosine by transferring a methyl group to the C5 position of cytosine. DNA methylation modulates gene expression by either attracting or preventing transcription factor(s) from binding to DNA. Objective: The purpose of this study is to figure out the connection between VDR-gene promoter methylation in Iraqi GDM and its consequence on vitamin D levels. Materials and Methods: This investigation comprised 50 patients with GDM (Group 1) and 30 healthy pregnant individuals as controls (Group 2). Methylation was analyzed by extracted DNA using Bisulfite conversion and detected by a specific primer. Results: The results of methylation effect on vitamin D3 expressed there were significant differences between methylated (25.74 ± 2.00) a percentage of 32(64.00%) of GDM samples compared with concentration (14.00±1.41) of methylated sample control at the percentage of observed results of methylation (1 (3.3%) at p-value (0.00) while the Un-methylated and partial methylate GDM samples did not produce any significant effect comparing with healthy controls. Conclusions: Due to the increased metabolic activity associated with pregnancy, it's imperative to keep glucose levels in balance. It is suspected that both genetic and epigenetic factors play a role in the development of GDM and that the underlying mechanisms are complex and alter over time. To create efficient treatments and preventative plans, a greater understanding of these mechanisms and how they affect GDM is needed.
{"title":"Associations of Epigenetic methylation with vitamin D receptor level in Iraqi Gestational diabetes mellitus patients","authors":"Yasameen A. Hadi, Risalla H. ALlami, Ahmed A. Suleiman","doi":"10.24126/jobrc.2023.17.2.734","DOIUrl":"https://doi.org/10.24126/jobrc.2023.17.2.734","url":null,"abstract":"Background :GDM (gestational diabetes mellitus) is a hazardous pregnancy situation that occurs when a pregnant woman who that have never had diabetes progresses to cause systemic hyperglycemia during their pregnancy.DNA methylation is an epigenetic mechanism it occurs in the vertebrate genomes and generates 5-methylcytosine by transferring a methyl group to the C5 position of cytosine. DNA methylation modulates gene expression by either attracting or preventing transcription factor(s) from binding to DNA. Objective: The purpose of this study is to figure out the connection between VDR-gene promoter methylation in Iraqi GDM and its consequence on vitamin D levels. Materials and Methods: This investigation comprised 50 patients with GDM (Group 1) and 30 healthy pregnant individuals as controls (Group 2). Methylation was analyzed by extracted DNA using Bisulfite conversion and detected by a specific primer. Results: The results of methylation effect on vitamin D3 expressed there were significant differences between methylated (25.74 ± 2.00) a percentage of 32(64.00%) of GDM samples compared with concentration (14.00±1.41) of methylated sample control at the percentage of observed results of methylation (1 (3.3%) at p-value (0.00) while the Un-methylated and partial methylate GDM samples did not produce any significant effect comparing with healthy controls. Conclusions: Due to the increased metabolic activity associated with pregnancy, it's imperative to keep glucose levels in balance. It is suspected that both genetic and epigenetic factors play a role in the development of GDM and that the underlying mechanisms are complex and alter over time. To create efficient treatments and preventative plans, a greater understanding of these mechanisms and how they affect GDM is needed.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139354897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-26DOI: 10.24126/jobrc.2023.17.2.658
Farah Essa, A. Al-Shammari, Ali Al-Saffar, Marwa Salman
Objective: The most recent World Health Organization statistics show that colorectal cancer is the third foremost cause of death by cancer among Iraqis. It has become necessary to search and develop novel and unconventional treatment methods. Oncolytic virotherapy is one of the innovative cancer therapeutics that showed to be safe and selective and there is increased efforts in the recent years to move them to clinic. For the reason we were developing our Iraqi strain of oncolytic NDV to fight colorectal cancer. Materials and Methods: This study tested the killing rate of Iraqi strain of Newcastle disease virus (AMHA1) on colorectal cancer cells and testing the safety on normal cells. The virus tittered using vero cells, and then the cytotoxic effect of the virus on both types of cells was determined using the crystal violet assay. Finally, we performed a morphological analysis using hematoxylin and eosin staining. Results: Newcastle disease virus 0.3 MOI causes significant cytotoxicity with prominent cytopathic effect in the colorectal cancer cell line HRT-18G with 50% percentage of growth inhibition, but not in the normal human fibroblast (NHF) percentage of growth inhibition where it was less than 20%. Conclusion oncolytic NDV AMHA1 strain effective against colorectal cancer cells because it has selectivity in replication and safety profile.
{"title":"The Oncolytic Effect of an Iraqi Newcastle Disease Virus Attenuated Strain (AMHA1) Against Colorectal Cancer Cells.","authors":"Farah Essa, A. Al-Shammari, Ali Al-Saffar, Marwa Salman","doi":"10.24126/jobrc.2023.17.2.658","DOIUrl":"https://doi.org/10.24126/jobrc.2023.17.2.658","url":null,"abstract":"Objective: The most recent World Health Organization statistics show that colorectal cancer is the third foremost cause of death by cancer among Iraqis. It has become necessary to search and develop novel and unconventional treatment methods. Oncolytic virotherapy is one of the innovative cancer therapeutics that showed to be safe and selective and there is increased efforts in the recent years to move them to clinic. For the reason we were developing our Iraqi strain of oncolytic NDV to fight colorectal cancer. Materials and Methods: This study tested the killing rate of Iraqi strain of Newcastle disease virus (AMHA1) on colorectal cancer cells and testing the safety on normal cells. The virus tittered using vero cells, and then the cytotoxic effect of the virus on both types of cells was determined using the crystal violet assay. Finally, we performed a morphological analysis using hematoxylin and eosin staining. Results: Newcastle disease virus 0.3 MOI causes significant cytotoxicity with prominent cytopathic effect in the colorectal cancer cell line HRT-18G with 50% percentage of growth inhibition, but not in the normal human fibroblast (NHF) percentage of growth inhibition where it was less than 20%. Conclusion oncolytic NDV AMHA1 strain effective against colorectal cancer cells because it has selectivity in replication and safety profile.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139354327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Clean hands are the single most important factor in preventing the spread of pathogens and reducing the incidence of infections. The good hand hygiene practices promote health safety and prevent infections. This study was carried out to assess the presence of bacteria from hands of Ambrose Alli University Students. Of all fifty (50) samples analyzed, at least one or two bacteria were isolated. The colony counts of students from each site were properly recorded with the highest found amongst dwellers of male hostels i.e 4.85 x 105cfu/ml, compared to dwellers at female hostels. However, there was considerable increase amongst students at Microbiology laboratory, which may be attributed to lack of good hand hygiene procedures not been adhered to. The considerable increase in the frequency of Escherichia coli and as well as Proteus sp is indicative of poor hygiene practices. The low colony counts recorded amongst students from school gate could be as a result of the student have taken their bath before coming to school. The frequency of occurrence of the isolates includes: Staphylococcus aureus 50 (100%), Escherichia coli 35 (70%), Klebsiella sp 15 (30%), Salmonella sp 21 (42%), Streptococcus sp 10 (20%), Bacillus sp 4 (8%), Pseudomonas sp 2 (4%) and Proteus sp 2 (4%). From the study, it was shown that there was considerable increase in poor hygienic practices amongst students of Ambrose Alli University, Ekpoma Edo State, Nigeria.
清洁双手是预防病原体传播和减少感染发生率的最重要因素。良好的手部卫生习惯可促进健康安全和预防感染。本研究旨在评估安布罗斯阿利大学学生手上的细菌存在情况。在分析的所有50个样本中，至少分离出一到两种细菌。每个地点的学生菌落计数都被正确记录下来，与女性宿舍的居民相比，男性宿舍的居民的菌落计数最高，为4.85 x 105cfu/ml。然而，在微生物实验室的学生中有相当大的增加，这可能是由于缺乏良好的手卫生程序，没有遵守。大肠杆菌和变形杆菌出现频率的大幅增加表明卫生习惯不良。在学校门口记录到的学生中菌落计数低可能是学生在上学前洗澡的结果。出现频率为:金黄色葡萄球菌50(100%)、大肠杆菌35(70%)、克雷伯氏菌15(30%)、沙门氏菌21(42%)、链球菌10(20%)、芽孢杆菌4(8%)、假单胞菌2(4%)和变形杆菌2(4%)。从这项研究中可以看出，尼日利亚埃克波马埃多州Ambrose Alli大学学生的不良卫生习惯有了相当大的增加。
{"title":"Bacteria Load Assessment from Hands of Students in a Tertiary University in South-South Nigeria","authors":"Iyevhobu Kenneth Oshiokhayamhe, Ken-Iyevhobu Benedicta Agumeile, Omolumen Lucky Eromosele, et. al.","doi":"10.32861/jbr.91.44.49","DOIUrl":"https://doi.org/10.32861/jbr.91.44.49","url":null,"abstract":"Clean hands are the single most important factor in preventing the spread of pathogens and reducing the incidence of infections. The good hand hygiene practices promote health safety and prevent infections. This study was carried out to assess the presence of bacteria from hands of Ambrose Alli University Students. Of all fifty (50) samples analyzed, at least one or two bacteria were isolated. The colony counts of students from each site were properly recorded with the highest found amongst dwellers of male hostels i.e 4.85 x 105cfu/ml, compared to dwellers at female hostels. However, there was considerable increase amongst students at Microbiology laboratory, which may be attributed to lack of good hand hygiene procedures not been adhered to. The considerable increase in the frequency of Escherichia coli and as well as Proteus sp is indicative of poor hygiene practices. The low colony counts recorded amongst students from school gate could be as a result of the student have taken their bath before coming to school. The frequency of occurrence of the isolates includes: Staphylococcus aureus 50 (100%), Escherichia coli 35 (70%), Klebsiella sp 15 (30%), Salmonella sp 21 (42%), Streptococcus sp 10 (20%), Bacillus sp 4 (8%), Pseudomonas sp 2 (4%) and Proteus sp 2 (4%). From the study, it was shown that there was considerable increase in poor hygienic practices amongst students of Ambrose Alli University, Ekpoma Edo State, Nigeria.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85216146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lumpy skin disease is characterized by its signs including eruptive, infectious and occasionally causing death to affected animals. It is caused by the family Poxviridea and genus Capripox with a strain of Neethling virus, a double stranded DNA virus. LSD clinical signs are mainly observed on skin as nodules (0.5 to 5 cm in diameter) throughout the skin surface or subcutaneous tissue involving superficial lymph nodes. Diagnosis of CaPV is based upon clinical signs with laboratory confirmation by virus isolation, polymerase chain reaction (PCR) and electron microscopy. Field diagnosis of LSD is often based on characteristic clinical signs of the disease. However, mild and subclinical forms require rapid and reliable laboratory testing to confirm diagnosis. Isolation of virus can be made from collected biopsy or at post-mortem from skin nodules, lung lesions or lymph nodes within the first week of the occurrence of clinical signs, before the development of neutralizing antibodies. There are different methods to detect LSD virus antibody from blood samples. These are serum neutralization test (SNT), indirect fluorescent antibody test (IFAT) and enzyme-linked immune-sorbent assay. LSD as a member of CaPVs, it has a single serotype, do not cause persistent infection, have a limited host range and vaccines are available that may provide long term immunity. These attributes increase the prospect of successfully implementing regional control programs, leading to the elimination of the virus and conceivably global eradication.
{"title":"Review on Lumpy Skin Disease Virus Characteristics and its Diagnosis in Cattle","authors":"S. Kassa","doi":"10.32861/jbr.91.33.43","DOIUrl":"https://doi.org/10.32861/jbr.91.33.43","url":null,"abstract":"Lumpy skin disease is characterized by its signs including eruptive, infectious and occasionally causing death to affected animals. It is caused by the family Poxviridea and genus Capripox with a strain of Neethling virus, a double stranded DNA virus. LSD clinical signs are mainly observed on skin as nodules (0.5 to 5 cm in diameter) throughout the skin surface or subcutaneous tissue involving superficial lymph nodes. Diagnosis of CaPV is based upon clinical signs with laboratory confirmation by virus isolation, polymerase chain reaction (PCR) and electron microscopy. Field diagnosis of LSD is often based on characteristic clinical signs of the disease. However, mild and subclinical forms require rapid and reliable laboratory testing to confirm diagnosis. Isolation of virus can be made from collected biopsy or at post-mortem from skin nodules, lung lesions or lymph nodes within the first week of the occurrence of clinical signs, before the development of neutralizing antibodies. There are different methods to detect LSD virus antibody from blood samples. These are serum neutralization test (SNT), indirect fluorescent antibody test (IFAT) and enzyme-linked immune-sorbent assay. LSD as a member of CaPVs, it has a single serotype, do not cause persistent infection, have a limited host range and vaccines are available that may provide long term immunity. These attributes increase the prospect of successfully implementing regional control programs, leading to the elimination of the virus and conceivably global eradication.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"196 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77438028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.24126/jobrc.2023.17.2.736
Zeena G. Faisal, K. H. Alobaidi, Mayaada Mahdi
Background: With growing environmental concern, interest was increased in research anddevelopment of environmentally friendly substances. Microbial surfactants are nontoxic andbiodegradable bioproducts that are widely used in medical, pharmaceutical, agricultural,industrial, food, cosmetics, and refinery applications. However, the main challenges in theproduction of biosurfactants are the high production cost and the limited yield. The improvementof biosurfactant production depend on many strategies, such as the use of low-cost raw materials,adjusting medium and growth conditions, and the selection of hype-producing strains.Objective: The study was conducted to obtain Pseudomonas guguanensis strain Iraqi ZG.K.Mmutant with higher biosurfactant production, through random mutagenesis.Materials and Methods: Chemical mutagen (acridine orange) in different concentrations andthe physical mutagen (UV irradiation) were used for the production of random mutagenesis inPseudomonas guguanensis strain Iraqi ZG.K.M. The biosurfactant hype-producing strain wasselected depending on CTAB test, E 24 %, and the measurement of surface tension.Results: A clear indication of the efficiency of UV light in producing biosurfactant mutant. Inwhich, a higher E 24 % (78%) and the lowest surface tension (33 mN/m) were recorded withZUVM2 mutant.Conclusion : UV light can successfully used to mutagenize Pseudomonas guguanensis strainIraqi ZG.K.M to obtain hyper-producer mutants of biosurfactant that used in many applications.
{"title":"Hyper-Production of Biosurfactant by Pseudomonas guguanensis Strain Iraqi ZG.K.M through Random Mutagenesis","authors":"Zeena G. Faisal, K. H. Alobaidi, Mayaada Mahdi","doi":"10.24126/jobrc.2023.17.2.736","DOIUrl":"https://doi.org/10.24126/jobrc.2023.17.2.736","url":null,"abstract":"Background: With growing environmental concern, interest was increased in research anddevelopment of environmentally friendly substances. Microbial surfactants are nontoxic andbiodegradable bioproducts that are widely used in medical, pharmaceutical, agricultural,industrial, food, cosmetics, and refinery applications. However, the main challenges in theproduction of biosurfactants are the high production cost and the limited yield. The improvementof biosurfactant production depend on many strategies, such as the use of low-cost raw materials,adjusting medium and growth conditions, and the selection of hype-producing strains.Objective: The study was conducted to obtain Pseudomonas guguanensis strain Iraqi ZG.K.Mmutant with higher biosurfactant production, through random mutagenesis.Materials and Methods: Chemical mutagen (acridine orange) in different concentrations andthe physical mutagen (UV irradiation) were used for the production of random mutagenesis inPseudomonas guguanensis strain Iraqi ZG.K.M. The biosurfactant hype-producing strain wasselected depending on CTAB test, E 24 %, and the measurement of surface tension.Results: A clear indication of the efficiency of UV light in producing biosurfactant mutant. Inwhich, a higher E 24 % (78%) and the lowest surface tension (33 mN/m) were recorded withZUVM2 mutant.Conclusion : UV light can successfully used to mutagenize Pseudomonas guguanensis strainIraqi ZG.K.M to obtain hyper-producer mutants of biosurfactant that used in many applications.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139364093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bo Peng Jing, Qiu Dong-Yan Kong Juan, Peng Yan Liu Gui-Ying, Guo Xiao-Yu Sun Xiang-Jin Xu Yan-Fang, Sun Rui-hua Pang Wei Zhou, Jin-Hui Zhao Quan-Xiu Wang
The protein content of rice seeds is an extremely important quality trait, but its genetic basis and molecular regulatory mechanism are still unclear. This study focuses on a positive regulatory gene OsAAP6 of grain protein content in rice. Proteins that interact with OsAAP6 were screened using yeast two hybrid experiments, and validated using in vivo point-to-point experiments and bimolecular fluorescence complementarity tests (BiFC). The main results are as follows: 98 positive colonies that may interact with OsAAP6 were screened from a rice cDNA library using yeast two hybrid technology. After sequencing and analysis, 40 proteins that may interact with OsAAP6 were ultimately obtained. Through comparative analysis, three proteins (Pyruvate phosphate dikinase (PPDR), WRKY, and GreA) were selected from these 40 proteins that may interact with each other. In vivo point-to-point experiments in yeast and bimolecular fluorescence complementarity (BiFC) experiments in rice were used to further verify that PPDK, WRKY, and GreA can interact with OsAAP6 protein, respectively. Therefore, the results of this study will provide important clues for further revealing the molecular mechanism by which the OsAAP6 gene regulates grain protein content.
{"title":"Screening and Validation of Rice OsAAP6 Interaction Protein","authors":"Bo Peng Jing, Qiu Dong-Yan Kong Juan, Peng Yan Liu Gui-Ying, Guo Xiao-Yu Sun Xiang-Jin Xu Yan-Fang, Sun Rui-hua Pang Wei Zhou, Jin-Hui Zhao Quan-Xiu Wang","doi":"10.32861/jbr.91.21.32","DOIUrl":"https://doi.org/10.32861/jbr.91.21.32","url":null,"abstract":"The protein content of rice seeds is an extremely important quality trait, but its genetic basis and molecular regulatory mechanism are still unclear. This study focuses on a positive regulatory gene OsAAP6 of grain protein content in rice. Proteins that interact with OsAAP6 were screened using yeast two hybrid experiments, and validated using in vivo point-to-point experiments and bimolecular fluorescence complementarity tests (BiFC). The main results are as follows: 98 positive colonies that may interact with OsAAP6 were screened from a rice cDNA library using yeast two hybrid technology. After sequencing and analysis, 40 proteins that may interact with OsAAP6 were ultimately obtained. Through comparative analysis, three proteins (Pyruvate phosphate dikinase (PPDR), WRKY, and GreA) were selected from these 40 proteins that may interact with each other. In vivo point-to-point experiments in yeast and bimolecular fluorescence complementarity (BiFC) experiments in rice were used to further verify that PPDK, WRKY, and GreA can interact with OsAAP6 protein, respectively. Therefore, the results of this study will provide important clues for further revealing the molecular mechanism by which the OsAAP6 gene regulates grain protein content.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82624139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-31DOI: 10.24126/jobrc.2023.17.1.682
K. Zainulabdeen
Background: The biological assessment of trimethoprim organotin complexes is the subject of the work discussed in this publication. Objective: The purpose of this project is to investigate the properties of trimethoprim tin complexes and via characterizing their IR and NMR elemental analysis sensitivity. Material and methods: These complexes combine octahedral ML6 and pyramidal ML5 tin complexes by allowing amino groups present in the medicinal ingredients to react with organotin (IV). Results: The complex conductivity was also determined for the prepared complexes and displayed low values. Discussions: Two concentrations of these complexes were screened for antibacterial activity against Gram+ve (Bacillus Pumilus) and (Candida albicans) by the Agar diffusion disc technique and showed a significant activity. In conclusion: the synthesized compounds proven to be more effective in inhibiting bacteria such as a) Bacillus Pumilus and b) Candida albicans at two concentrations (1×10-2, 1×10-4M).
{"title":"Anti-bacterial activity for trimethoprim and its complexes","authors":"K. Zainulabdeen","doi":"10.24126/jobrc.2023.17.1.682","DOIUrl":"https://doi.org/10.24126/jobrc.2023.17.1.682","url":null,"abstract":"Background: The biological assessment of trimethoprim organotin complexes is the subject of the work discussed in this publication. Objective: The purpose of this project is to investigate the properties of trimethoprim tin complexes and via characterizing their IR and NMR elemental analysis sensitivity. Material and methods: These complexes combine octahedral ML6 and pyramidal ML5 tin complexes by allowing amino groups present in the medicinal ingredients to react with organotin (IV). Results: The complex conductivity was also determined for the prepared complexes and displayed low values. Discussions: Two concentrations of these complexes were screened for antibacterial activity against Gram+ve (Bacillus Pumilus) and (Candida albicans) by the Agar diffusion disc technique and showed a significant activity. In conclusion: the synthesized compounds proven to be more effective in inhibiting bacteria such as a) Bacillus Pumilus and b) Candida albicans at two concentrations (1×10-2, 1×10-4M).","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80094047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food waste (FW) is one of the main problems in the world due to the continuous increase in the global population. Anaerobic digestion (AD) of FW was an alternative and economical solution to develop an effective method to enhance biomethane (BioM) production that uses enzyme pretreatment and hydrolysis of FW by locally produced cellulase and amylase enzymes. In this study, two types of sources fungi (TNAF-1 to TNFA-3) and (TNBC-1 to TNBC-3) strains were isolated from animal feed and compost. The cellulase and amylase activities were 300U/mL & 400U/mL, respectively. Based on OFAT results obtained, optimization of three factors such as pH of 5, TS of 12.5% (V/V) and enzyme loading of 80U/mL was carried out by applying the FCCCD under the RSM to develop a second-order regression model successful improvement in the production of reducing sugar of 162mg/mL was achieved. However, biogas yield was optimum using OFAT parameters such as the biogas inoculum of 25%, pH of 7, AD digestion times of 29 days, 500mL of hydrolysate food waste and room temperature at 30°C (±2). The results show the biogas contained was found such %3 hydrogens, 57% methane and 40% carbon dioxide. The new fungi are very potential for upgrading the biogas of BioM that is non-toxic as well as biodegradable, and therefore may be encouraging to the water treatment plants in future applications.
{"title":"Anaerobic Digestion for Biomethane Production from Food Waste Pretreated by Enzymatic Hydrolysis","authors":"N. Deb, M. Alam, Tawfkur Rahman, et. al.","doi":"10.32861/jbr.91.6.20","DOIUrl":"https://doi.org/10.32861/jbr.91.6.20","url":null,"abstract":"Food waste (FW) is one of the main problems in the world due to the continuous increase in the global population. Anaerobic digestion (AD) of FW was an alternative and economical solution to develop an effective method to enhance biomethane (BioM) production that uses enzyme pretreatment and hydrolysis of FW by locally produced cellulase and amylase enzymes. In this study, two types of sources fungi (TNAF-1 to TNFA-3) and (TNBC-1 to TNBC-3) strains were isolated from animal feed and compost. The cellulase and amylase activities were 300U/mL & 400U/mL, respectively. Based on OFAT results obtained, optimization of three factors such as pH of 5, TS of 12.5% (V/V) and enzyme loading of 80U/mL was carried out by applying the FCCCD under the RSM to develop a second-order regression model successful improvement in the production of reducing sugar of 162mg/mL was achieved. However, biogas yield was optimum using OFAT parameters such as the biogas inoculum of 25%, pH of 7, AD digestion times of 29 days, 500mL of hydrolysate food waste and room temperature at 30°C (±2). The results show the biogas contained was found such %3 hydrogens, 57% methane and 40% carbon dioxide. The new fungi are very potential for upgrading the biogas of BioM that is non-toxic as well as biodegradable, and therefore may be encouraging to the water treatment plants in future applications.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74073418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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