Pub Date : 2021-12-01DOI: 10.24126/jobrc.2021.15.2.608
K. Abdullah, Laith Muslah Najeeb, Ezeddin Albayar
The current investigation was intended to assess the viability of sesame oil got from Heet city and Indian premium (saussurea costus) affectability testing against Acinetobacter baumanii isolates detached from disease destination like urine, sputum, C.S.F, wound swab, and burn swab. The results indicated that sesame oil activated the gentamycin antibiotic activity from 3.08 mm to 14.7500 mm and also the Indian premium made the same thing the activation was from 5.4167 mm to 9.3333 mm. while the addition of sesame oil to Imipenem anti biotic there was inhibition in the activity of Imipenem from 21.33 mm to 20.083mm and when we added the Indian premium there was activation to the Imipenem antibiotic was from 22.1667 mm to 23.4167mm. Finally the results of cefotaxime after adding the sesame oil we noticed that there is decrease in the activity of cefotaxime from 3.500 mm to 1.000 mm and we also noticed that the addition of Indian premium to the antibiotic there was decrease in the activity from 3.9167 mm to 3.333 mm. Therefore ower explanation that there is activation to some antibiotics there is also inhibition to the other by adding different plant extract.
本研究旨在评估从艾特市和印度premium (saussurea costus)中提取的芝麻油对尿、痰、csf、伤口拭子和烧伤拭子等疾病目的地分离的鲍曼不动杆菌的敏感性试验的可行性。结果表明，芝麻油对庆大霉素活性的激活范围为3.08 mm ~ 14.7500 mm，印度油对庆大霉素活性的激活范围为5.4167 mm ~ 9.3333 mm，而亚胺培南对亚胺培南活性的抑制范围为21.33 mm ~ 20.083mm，印度油对亚胺培南活性的激活范围为22.1667 mm ~ 23.4167mm。最后，在加入香油后的头孢噻肟的结果中，我们注意到头孢噻肟的活性从3.500 mm下降到1.000 mm，我们还注意到，在抗生素中加入印度premium后，活性从3.9167 mm下降到3.333 mm。因此更有可能的解释是，加入不同的植物提取物对某些抗生素有激活作用，对另一些抗生素也有抑制作用。
{"title":"Study the effect of Indian premium (Saussurea costus) extract and sesame oil on the sensitivity of local Acinetobacter baumannii isolates causing infection","authors":"K. Abdullah, Laith Muslah Najeeb, Ezeddin Albayar","doi":"10.24126/jobrc.2021.15.2.608","DOIUrl":"https://doi.org/10.24126/jobrc.2021.15.2.608","url":null,"abstract":" The current investigation was intended to assess the viability of sesame oil got from Heet city and Indian premium (saussurea costus) affectability testing against Acinetobacter baumanii isolates detached from disease destination like urine, sputum, C.S.F, wound swab, and burn swab. The results indicated that sesame oil activated the gentamycin antibiotic activity from 3.08 mm to 14.7500 mm and also the Indian premium made the same thing the activation was from 5.4167 mm to 9.3333 mm. while the addition of sesame oil to Imipenem anti biotic there was inhibition in the activity of Imipenem from 21.33 mm to 20.083mm and when we added the Indian premium there was activation to the Imipenem antibiotic was from 22.1667 mm to 23.4167mm. Finally the results of cefotaxime after adding the sesame oil we noticed that there is decrease in the activity of cefotaxime from 3.500 mm to 1.000 mm and we also noticed that the addition of Indian premium to the antibiotic there was decrease in the activity from 3.9167 mm to 3.333 mm. \u0000Therefore ower explanation that there is activation to some antibiotics there is also inhibition to the other by adding different plant extract.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85634146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.24126/jobrc.2021.15.2.604
A. F. Salman, B. Hameed, E. A. Ali
To assess the significance of platelet indices and the ratio of platelet to lymphocyte as an early prediction of patients with severity of Preeclampsia. Ninety women were enrolled in three groups: group I: (In=30) women diagnosed as non-severe preeclampsia, group II: (n=30) women diagnosed as severe preeclampsia, and group: III (n=30) age-matched normal pregnant women as a control group. The platelet parameters namely: platelet count (PC), mean platelet volume (MPV), platelet distribution width (PDW), and platelet to lymphocyte ratio (P/L ratio) were evaluated and compared among the study groups.Our findings showed a significant difference among the studied groups regarding the followings: body mass index BMI, systolic blood pressure (SBP) and diastolic blood pressure (DBP) at P-value < 0.05. Mean Platelet Volume and Platelet Distribution Width showed no significant differences between the study groups (P= 0.693 and 0.118, respectively). In all groups, highly decrease in platelet count (p = 0.01) and platelet to lymphocyte ratio (p= 0.024) were found. The changes in the platelet indices are more evident in mothers with severe preeclampsia especially the ratio between platelet and lymphocyte. Thus, Platelet indices could be used as biomarkers for early prediction of preeclampsia seriousness.
{"title":"The Value of Platelet Indices and platelet to lymphocyte ratio as predictors of severity of Preeclampsia in Iraqi women","authors":"A. F. Salman, B. Hameed, E. A. Ali","doi":"10.24126/jobrc.2021.15.2.604","DOIUrl":"https://doi.org/10.24126/jobrc.2021.15.2.604","url":null,"abstract":" To assess the significance of platelet indices and the ratio of platelet to lymphocyte as an early prediction of patients with severity of Preeclampsia. Ninety women were enrolled in three groups: group I: (In=30) women diagnosed as non-severe preeclampsia, group II: (n=30) women diagnosed as severe preeclampsia, and group: III (n=30) age-matched normal pregnant women as a control group. The platelet parameters namely: platelet count (PC), mean platelet volume (MPV), platelet distribution width (PDW), and platelet to lymphocyte ratio (P/L ratio) were evaluated and compared among the study groups.Our findings showed a significant difference among the studied groups regarding the followings: body mass index BMI, systolic blood pressure (SBP) and diastolic blood pressure (DBP) at P-value < 0.05. Mean Platelet Volume and Platelet Distribution Width showed no significant differences between the study groups (P= 0.693 and 0.118, respectively). In all groups, highly decrease in platelet count (p = 0.01) and platelet to lymphocyte ratio (p= 0.024) were found. The changes in the platelet indices are more evident in mothers with severe preeclampsia especially the ratio between platelet and lymphocyte. Thus, Platelet indices could be used as biomarkers for early prediction of preeclampsia seriousness. \u0000 ","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91044046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.24126/jobrc.2021.15.2.606
A. Hamied
The occurrences of invasive candidiasis has increased over the previous few decades. Although Candida albicans considers as one of the most common species of organisms, that cause acquired fungal infections. Candida albicans is an opportunistic fungal pathogen and inherent in as a lifelong, the yeast is present in healthy individuals as a commensal, and can reside harmlessly in human body. However, in immuno-compromised individuals, the fungus can invade tissues, producing superficial infections and, in severe cases, life-threatening systemic infections. This review wills emphasis on virulence factor of C. albicans including (adhesion, invasion, candida proteinase, and phenotypic switching and biofilm formation. Invasive Fungal diseases are result in very high morbidity as well as up to 60% mortality for people with severely susceptible hosts. This review will illustrate briefly the virulence factors in Candida albicans.
{"title":"Candida and Candidiasis - A review of Virulence Factors","authors":"A. Hamied","doi":"10.24126/jobrc.2021.15.2.606","DOIUrl":"https://doi.org/10.24126/jobrc.2021.15.2.606","url":null,"abstract":" The occurrences of invasive candidiasis has increased over the previous few decades. Although Candida albicans considers as one of the most common species of organisms, that cause acquired fungal infections. \u0000Candida albicans is an opportunistic fungal pathogen and inherent in as a lifelong, the yeast is present in healthy individuals as a commensal, and can reside harmlessly in human body. However, in immuno-compromised individuals, the fungus can invade tissues, producing superficial infections and, in severe cases, life-threatening systemic infections. This review wills emphasis on virulence factor of C. albicans including (adhesion, invasion, candida proteinase, and phenotypic switching and biofilm formation. \u0000Invasive Fungal diseases are result in very high morbidity as well as up to 60% mortality for people with severely susceptible hosts. This review will illustrate briefly the virulence factors in Candida albicans. \u0000 ","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76205563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Black rice is the main member of the special rice family, and it is also a rare grain and oil crop resource in China. Black rice has special medicinal components and high nutritional value. In recent years, the research on the nutritional value and application of black rice has become an important direction, and a series of important progress has been made. In this paper, the nutrition and its influencing factors of black rice, the main ways to improve eating quality, the health care and medical functions of black rice were reviewed. Meanwhile, the application prospect of black rice has also been prospected. Therefore, this review will provide an important theoretical basis for genetic improvement and breeding of new varieties of black rice.
{"title":"The Nutritional Value and Application of Black Rice-A Review","authors":"Bo Peng, Anqi Lou, Xiaoyan Luo, Rui Wang, Shuangyan Tu, Ziyan Xue, Jing Qiu, Xiayu Tian, Fang Yang, Yan-ming Zhang, Ya-Qin Huang, Yan-Yang Sun, Ping-Li Chen, Wei Zhou, Quanxiu Wang","doi":"10.32861/jbr.74.63.72","DOIUrl":"https://doi.org/10.32861/jbr.74.63.72","url":null,"abstract":"Black rice is the main member of the special rice family, and it is also a rare grain and oil crop resource in China. Black rice has special medicinal components and high nutritional value. In recent years, the research on the nutritional value and application of black rice has become an important direction, and a series of important progress has been made. In this paper, the nutrition and its influencing factors of black rice, the main ways to improve eating quality, the health care and medical functions of black rice were reviewed. Meanwhile, the application prospect of black rice has also been prospected. Therefore, this review will provide an important theoretical basis for genetic improvement and breeding of new varieties of black rice.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87702266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The indigenous knowledge of medicinal plants appeared when humans started and learned how to use the traditional knowledge of medicinal plants. The various literature available shows the significant role of medicinal plants in primary health care delivery in Ethiopia (about 70% of humans and 90% of livestock population) depend on traditional plant medicine. This is similar to many developing countries, particularly with those countries in Sub-saharan Africa. Medicinal plants provide a vital contribution to human and livestock health care. This seminar review was an attempt to present five medicinal plants (citrus lemon, caricapapaya, Moringa stenopetala, Allium sativum, Zingiber officinale).
{"title":"A Seminar Paper on the Review of Five well-known Medicinal Plants Used in Ethiopia","authors":"Gadissa Melkamu Bulcha","doi":"10.32861/JBR.73.51.56","DOIUrl":"https://doi.org/10.32861/JBR.73.51.56","url":null,"abstract":"The indigenous knowledge of medicinal plants appeared when humans started and learned how to use the traditional knowledge of medicinal plants. The various literature available shows the significant role of medicinal plants in primary health care delivery in Ethiopia (about 70% of humans and 90% of livestock population) depend on traditional plant medicine. This is similar to many developing countries, particularly with those countries in Sub-saharan Africa. Medicinal plants provide a vital contribution to human and livestock health care. This seminar review was an attempt to present five medicinal plants (citrus lemon, caricapapaya, Moringa stenopetala, Allium sativum, Zingiber officinale).","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88823259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iyevhobu Kenneth Oshiokhayamhe, R. A. Amaechi, A. Turay, T. Okobi, E. Usoro, B A Ken-Iyevhobu
An estimated 50,000 Nigerian women die each year from complications of pregnancy and childbirth, accounting for 10% of global estimates of pregnancy maternal death with about 2% resulting from drug induction. This cross-sectional study sets out to evaluate the Prothrombin time test (PT), activated partial thromboplastin time test (aPTT) Erythrocyte sedimentation rate (ESR), and Platelet count (PC) of pregnant women attending antenatal clinics at Oredo Health Centre in Benin City, Edo State. A total number of 130 subjects comprising 100 pregnant women and 30 non-pregnant women were recruited for the study. Prothrombin time (PT), Activated Partial Thromboplastin Time (APTT), Platelet count and Erythrocyte Sedimentation Rate (ESR) were studied using standard manual methods. The prothrombin time (sec) of the pregnant women 1st trimester (19.12±0.77b), 2nd trimester (19.90±1.02 b) and 3rd trimester (19.66±0.56 b), activated partial thromboplastin time (sec) 1st trimester (44.02±1.17 b), 2nd trimester (47.72±1.47 b) and 3rd trimester (45.88±1.10b), Erythrocyte sedimentation rate (mm/hr) 1st trimester (24.37±3.04 a), 2nd trimester (37.83±4.53 a) and 3rd trimester (43.25±5.24 a) and platelet count (X109/L) 1st trimester (248.29±23.18a), 2nd trimester (236.33±13.84 b) and 3rd trimester (239.10±16.07 a) were significantly higher than the prothrombin time (sec) 16.48±0.81 a, activated partial thromboplastin time (sec) 36.53±1.42 a, ESR (mm/hr) 29.83±4.14 a and platelet count (X109/L) 201±9.54 an of the non-pregnant women (p<0.05). The ESR (mm/hr) of the 3rd trimester (43.25±5.24) was observed to be significantly higher than that of the 1st trimester (24.37±3.04) and 2nd trimester (37.83±4.53) (p<0.05). Our investigation showed that antibiotics in pregnancy have a deleterious effect on PT, ESR, APTT and PC studied. We recommend that pregnant women should be given due attention throughout the course and events of pregnancy to prevent or reduce the risk of thrombotic episodes and possible disseminated intravascular coagulation (DIC) with resultant better maternity/child safety and health outcome and educated on the effects of antibiotics to pregnancy.
{"title":"Effects of Antibiotics on Haemostatic Parameters during Pregnancy","authors":"Iyevhobu Kenneth Oshiokhayamhe, R. A. Amaechi, A. Turay, T. Okobi, E. Usoro, B A Ken-Iyevhobu","doi":"10.32861/JBR.73.57.62","DOIUrl":"https://doi.org/10.32861/JBR.73.57.62","url":null,"abstract":"An estimated 50,000 Nigerian women die each year from complications of pregnancy and childbirth, accounting for 10% of global estimates of pregnancy maternal death with about 2% resulting from drug induction. This cross-sectional study sets out to evaluate the Prothrombin time test (PT), activated partial thromboplastin time test (aPTT) Erythrocyte sedimentation rate (ESR), and Platelet count (PC) of pregnant women attending antenatal clinics at Oredo Health Centre in Benin City, Edo State. A total number of 130 subjects comprising 100 pregnant women and 30 non-pregnant women were recruited for the study. Prothrombin time (PT), Activated Partial Thromboplastin Time (APTT), Platelet count and Erythrocyte Sedimentation Rate (ESR) were studied using standard manual methods. The prothrombin time (sec) of the pregnant women 1st trimester (19.12±0.77b), 2nd trimester (19.90±1.02 b) and 3rd trimester (19.66±0.56 b), activated partial thromboplastin time (sec) 1st trimester (44.02±1.17 b), 2nd trimester (47.72±1.47 b) and 3rd trimester (45.88±1.10b), Erythrocyte sedimentation rate (mm/hr) 1st trimester (24.37±3.04 a), 2nd trimester (37.83±4.53 a) and 3rd trimester (43.25±5.24 a) and platelet count (X109/L) 1st trimester (248.29±23.18a), 2nd trimester (236.33±13.84 b) and 3rd trimester (239.10±16.07 a) were significantly higher than the prothrombin time (sec) 16.48±0.81 a, activated partial thromboplastin time (sec) 36.53±1.42 a, ESR (mm/hr) 29.83±4.14 a and platelet count (X109/L) 201±9.54 an of the non-pregnant women (p<0.05). The ESR (mm/hr) of the 3rd trimester (43.25±5.24) was observed to be significantly higher than that of the 1st trimester (24.37±3.04) and 2nd trimester (37.83±4.53) (p<0.05). Our investigation showed that antibiotics in pregnancy have a deleterious effect on PT, ESR, APTT and PC studied. We recommend that pregnant women should be given due attention throughout the course and events of pregnancy to prevent or reduce the risk of thrombotic episodes and possible disseminated intravascular coagulation (DIC) with resultant better maternity/child safety and health outcome and educated on the effects of antibiotics to pregnancy.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83460864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Viruses are microscopic organisms that can only grow within living host cells. Various types of animals such as Rats, Bat, Cows, snakes, and related wild animals can serve as vehicles of transmission of this group of organisms. Novel SARS-CoV-2 belongs to the Coronaviridae family that was reported to have emanated from Wuhan, China in December 2019. It alerts world response against strange viral respiratory disease. As the world reflects on pandemics historically. Epidemics like the London plague of 1665 and the Plague that emanates from Marseille, France of 1720 claimed millions of lives. Among the most dreadful world pandemics are the “Antonine Plague” which occurred in 165AD and claimed 5 million lives in Egypt, Asia, Italy, and Greece. “Plague of Justinian” was reported to have occurred between the years 541 and 542. In addition to this, “The Flu Pandemic” is also referred to as Spanish flu ravaged the world during the year 1918 to 1920, which is about a century ago.”3rd Plague of 1855” originated from China and was responsible for the death of 10 million Indians within one year.” The Black Death” which is a 7 years long pandemic devastated the world between the years 1346 to 1353. It leads to the death of half of the global population and was by far the worst pandemic. HIV-AIDS has peak record deaths between 2005-2012. In the year 2019/2020 COVID-19 broke out severely than MERS-CoV and SARS-CoV which occurred in recent years. One possible best approach for the control of COVID-19 diseases caused by SARS-CoV-2 is to improve the hosts’ immune system. Evidence shows that inflammatory conditions militate against the immune systems of COVID-19 patients. Some other control strategies will include the use of face masks, physical distancing, hand washing, or use of alcohol-based sanitizers and related personal hygiene are important to contain the diseases.
{"title":"Milestone of World Pandemics: A Review on Remedy for COVID-19 Diseases to Revitalize Human Race from Deadly Corona Virus","authors":"A. O. Ajayi","doi":"10.32861/JBR.72.27.33","DOIUrl":"https://doi.org/10.32861/JBR.72.27.33","url":null,"abstract":"Viruses are microscopic organisms that can only grow within living host cells. Various types of animals such as Rats, Bat, Cows, snakes, and related wild animals can serve as vehicles of transmission of this group of organisms. Novel SARS-CoV-2 belongs to the Coronaviridae family that was reported to have emanated from Wuhan, China in December 2019. It alerts world response against strange viral respiratory disease. As the world reflects on pandemics historically. Epidemics like the London plague of 1665 and the Plague that emanates from Marseille, France of 1720 claimed millions of lives. Among the most dreadful world pandemics are the “Antonine Plague” which occurred in 165AD and claimed 5 million lives in Egypt, Asia, Italy, and Greece. “Plague of Justinian” was reported to have occurred between the years 541 and 542. In addition to this, “The Flu Pandemic” is also referred to as Spanish flu ravaged the world during the year 1918 to 1920, which is about a century ago.”3rd Plague of 1855” originated from China and was responsible for the death of 10 million Indians within one year.” The Black Death” which is a 7 years long pandemic devastated the world between the years 1346 to 1353. It leads to the death of half of the global population and was by far the worst pandemic. HIV-AIDS has peak record deaths between 2005-2012. In the year 2019/2020 COVID-19 broke out severely than MERS-CoV and SARS-CoV which occurred in recent years. One possible best approach for the control of COVID-19 diseases caused by SARS-CoV-2 is to improve the hosts’ immune system. Evidence shows that inflammatory conditions militate against the immune systems of COVID-19 patients. Some other control strategies will include the use of face masks, physical distancing, hand washing, or use of alcohol-based sanitizers and related personal hygiene are important to contain the diseases.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84647234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.24126/JOBRC.2021.15.1.596
Miriam Jasim Shehab, M. Sabbah, D. S. Namaa, Sura Nabeel
Analysis of touched DNA from crime scenes is fundamental in forensic DNA laboratories. Many factors affect the recovery of DNA from touched surfaces and then affect the quality of the final results. The aim of this work is studying the possibility of recovery suitable amount of DNA from ltouched portable computer. The computer was cleaned with 10% Bleach then touch and DNA collected for extraction by Organic method and two STR regions D5S818 (115-163bp) and FGA (308-464bp) were amplified. The results showed that it is possible to isolate a proper amount of DNA from touched portable computer where it was amplified and then analyzed by Agarose gel electrophoresis. The conclusion is that portable computer is suitable source for forensic analysis.
{"title":"Isolation and Amplification of Touch DNA from Portable Computer","authors":"Miriam Jasim Shehab, M. Sabbah, D. S. Namaa, Sura Nabeel","doi":"10.24126/JOBRC.2021.15.1.596","DOIUrl":"https://doi.org/10.24126/JOBRC.2021.15.1.596","url":null,"abstract":" Analysis of touched DNA from crime scenes is fundamental in forensic DNA laboratories. Many factors affect the recovery of DNA from touched surfaces and then affect the quality of the final results. The aim of this work is studying the possibility of recovery suitable amount of DNA from ltouched portable computer. The computer was cleaned with 10% Bleach then touch and DNA collected for extraction by Organic method and two STR regions D5S818 (115-163bp) and FGA (308-464bp) were amplified. The results showed that it is possible to isolate a proper amount of DNA from touched portable computer where it was amplified and then analyzed by Agarose gel electrophoresis. The conclusion is that portable computer is suitable source for forensic analysis.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73786153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bo Peng, Qing-xi Zhang, Xiayu Tian, Yanfang Sun, Xin-Hua Huang, Rui-hua Pang, Quanxiu Wang, Wei Zhou, Hongyu Yuan, Fang Yang, Juan Peng, Hui Song, A. Xin-Xiang
Rice is one of the most important food crops in the world, and about half of the world’s population uses it as the main food source. China’s annual rice output accounts for about 34% of the world’s annual rice output, ranking first in the world, thus China is known as the "Rice Kingdom". Rice has high nutritional value, providing the human body with 35% of the total energy intake and about 28% of the required protein. Therefore, the nutritional value of rice is directly related to human nutrition and health. In this paper, the three aspects of rice nutrition and human health, factors affecting rice nutritional quality and genetic improvement of nutritional quality of rice are reviewed. The new challenges of rice nutrition quality were analyzed, and the prospect of improving rice nutritional quality was prospected. The results provide theoretical basis for genetic improvement of rice nutrition quality and cultivation of new high-quality rice varieties in the future.
{"title":"Influencing Factors of Grain Nutritional Quality and its Genetic Improvement Strategy in Rice","authors":"Bo Peng, Qing-xi Zhang, Xiayu Tian, Yanfang Sun, Xin-Hua Huang, Rui-hua Pang, Quanxiu Wang, Wei Zhou, Hongyu Yuan, Fang Yang, Juan Peng, Hui Song, A. Xin-Xiang","doi":"10.32861/jbr.71.1.11","DOIUrl":"https://doi.org/10.32861/jbr.71.1.11","url":null,"abstract":"Rice is one of the most important food crops in the world, and about half of the world’s population uses it as the main food source. China’s annual rice output accounts for about 34% of the world’s annual rice output, ranking first in the world, thus China is known as the \"Rice Kingdom\". Rice has high nutritional value, providing the human body with 35% of the total energy intake and about 28% of the required protein. Therefore, the nutritional value of rice is directly related to human nutrition and health. In this paper, the three aspects of rice nutrition and human health, factors affecting rice nutritional quality and genetic improvement of nutritional quality of rice are reviewed. The new challenges of rice nutrition quality were analyzed, and the prospect of improving rice nutritional quality was prospected. The results provide theoretical basis for genetic improvement of rice nutrition quality and cultivation of new high-quality rice varieties in the future.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89137984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The inhibitory effects of five commercial extracts (Allium sativum, Chamaemelum nobile, Thymus vulgaris, Zingiber officinale and Ricinus communis ) against Alternaria alternata were tested three concentrations (2.5, 5, 12.5 µl/ml) in vitro. T. vulgaris and R.communis extracts both exhibited the most effective antifungal activity against A. alternate with diameter of inhibition zones of 54 mm. The C. nobile extract exhibited a lower degree of inhibition 24.5 to 45 mm at three concentrations. The antifungal indices of T. vulgaris and R.communis extracts at three concentrations against A. alternata were all 98.14%, while this of C. nobile extract was 43.52% at 12.5 µl/ml. The results show that the five commercial extracts have potential for the development of natural antifungal agents, which could be an alternative to chemicals for control of phytopathogenic fungi on fruits or vegetables.
{"title":"Antifungal Activities of Five Commercial Extracts Against Alternaria alternate","authors":"B. Ghalem, B. Talia, H. Omar","doi":"10.32861/jbr.68.98.103","DOIUrl":"https://doi.org/10.32861/jbr.68.98.103","url":null,"abstract":"The inhibitory effects of five commercial extracts (Allium sativum, Chamaemelum nobile, Thymus vulgaris, Zingiber officinale and Ricinus communis ) against Alternaria alternata were tested three concentrations (2.5, 5, 12.5 µl/ml) in vitro. T. vulgaris and R.communis extracts both exhibited the most effective antifungal activity against A. alternate with diameter of inhibition zones of 54 mm. The C. nobile extract exhibited a lower degree of inhibition 24.5 to 45 mm at three concentrations. The antifungal indices of T. vulgaris and R.communis extracts at three concentrations against A. alternata were all 98.14%, while this of C. nobile extract was 43.52% at 12.5 µl/ml. The results show that the five commercial extracts have potential for the development of natural antifungal agents, which could be an alternative to chemicals for control of phytopathogenic fungi on fruits or vegetables.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85478879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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