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Biocompatibility and expression of transcription factors of a type B gelatin-Extracellular Matrix of Porcin Urinary Blader scaffold. B 型明胶-Porcin 尿道膀胱支架细胞外基质的生物相容性和转录因子的表达。
IF 2.3 4区 医学 Q3 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-01 Epub Date: 2024-07-29 DOI: 10.1177/08853282241267867
Olivia Abril Cuevas-Tapia, Mariana Gutiérrez-Sánchez, Amaury Pozos-Guillén, Juan Valerio Cauich-Rodríguez, Diana María Escobar-García

Objective: to evaluate a membrane based on type B gelatin (G) and porcine urinary bladder extracellular matrix (PUB-EM), highlighting the potential effect of the combination evaluated by biocompatibility and regulation of the expression of transcription factors involved in tissue regeneration. G-PUB-EM membranes were prepared at 12.5, 25, and 50% w/v, and evaluated for biocompatibility with Fibroblast. Chemical characterization by FTIR-ATR showed complex spectra during crosslinking process with glutaraldehyde. Physical tests were performed in deionized water and PBS for 48 h. A significant increase in swelling was observed during the first 2 h. Biocompatibility testing (MTS) and evaluation of the expression profile of genes involved in the cell cycle (Cyclin-D1 VEGF, TNF and NF-κ-B) by PCR showed an increase in viability in a PUB-EM content-dependent way, except for 50% PUB-EM membrane which showed cytotoxic effects with a decrease in cell viability below 70%. The membranes showed an increase in the expression of some factors of cell cycle, as well as inflammatory processes that could promote tissue repair. 12.5 and 25% gelatin type B/porcine urinary bladder extracellular matrix (G/PUB-EM) based membranes have potential for tissue regeneration applications.

Impact statement: The use of membranes based on type B gelatin and porcine urinary bladder for tissue engineering represents a novel strategy. Biocompatibility and signaling pathways play a primary role in tissue repair and wound recovery. Transcription factors that mediate signaling, cell division and vascularization are part of molecules that intervene in the regenerative potential of cells. These techniques will have a significant impact on tissue repair and regeneration and thus stop depending on tissue donors or other surgical sites from the same patient, as is the case with burn patients.

目的:评估一种基于 B 型明胶(G)和猪膀胱细胞外基质(PUB-EM)的膜,通过生物相容性和对参与组织再生的转录因子表达的调节,突出评估两者结合的潜在效果。以 12.5、25 和 50% w/v 的浓度制备 G-PUB-EM 膜,并评估其与成纤维细胞的生物相容性。通过傅立叶变换红外-原子吸收光谱(FTIR-ATR)进行的化学特性分析表明,在与戊二醛交联的过程中会出现复杂的光谱。生物相容性测试(MTS)和 PCR 对细胞周期相关基因(Cyclin-D1、VEGF、TNF 和 NF-κ-B)表达谱的评估显示,细胞存活率的提高与 PUB-EM 的含量有关,但 50%的 PUB-EM 膜除外,它具有细胞毒性,细胞存活率低于 70%。这些膜显示细胞周期中某些因子的表达增加,炎症过程也增加,从而促进了组织修复。基于12.5%和25%明胶B型/猪膀胱细胞外基质(G/PUB-EM)的膜具有组织再生应用的潜力:将基于B型明胶和猪膀胱的膜用于组织工程是一种新策略。生物相容性和信号通路在组织修复和伤口恢复中发挥着主要作用。介导信号传导、细胞分裂和血管生成的转录因子是干预细胞再生潜能的部分分子。这些技术将对组织修复和再生产生重大影响,从而不再像烧伤病人那样依赖组织捐献者或同一病人的其他手术部位。
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引用次数: 0
A novel biological antibacterial polyvinyl alcohol/polyionic liquid hydrogel for wound dressing. 用于伤口敷料的新型生物抗菌聚乙烯醇/聚阴离子液态水凝胶。
IF 2.3 4区 医学 Q3 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-01 Epub Date: 2024-06-20 DOI: 10.1177/08853282241264095
Wang Liu, Hao Wang, Jiaqi Liu, Yuen Y Cheng, Yanchun Guan, Kedong Song

The release of antibiotics or anions by traditional bacteriostatic agents led to the development of bacterial drug resistance and environmental pollution. Ionic liquids (ILs) have become important choices for antibacterial agents because of their excellent physical, chemical and biological properties. In this paper, the bioactivities of 1-vinyl-3-butylimidazolium chloride ([VBIM]Cl, IL) and poly (1-vinyl-3-butylimidazolium chloride) (P[VBIM]Cl, PIL) were evaluated, and the potential antibacterial material was used to synthesize hydrogels. Using the colony formation assay and the Oxford cup method, antibacterial effect of IL and PIL were tested. Cell-Counting-Kit-8 (CCK-8) experiments were used to study the IC50 (half maximal inhibitory concentration) values of IL and showed 1.47 mg/mL, 0.35 mg/mL and 0.33 mg/mL at 24 h, 48 h and 72 h, respectively. The IC50 value of PIL were 12.15 μg/mL, 12.06 μg/mL and 11.76 μg/mL at 24 h, 48 h and 72 h, respectively. The PIL is further crosslinked with polyvinyl alcohol (PVA) to form a novel hydrogel through freeze-thaw cycles. The newly fabricated hydrogel exhibited a high water content, excellent water absorption properties and outstanding mechanical performance. Using the colony formation assay and the inhibition zone assay, the hydrogels exhibited favorable antibacterial effects (against E.coli and S.aureus) such that nearly 100% of the bacteria were killed in liquid medium while cultivating with H4 (synthesized by 0.5 g PIL and 1g PVA). In addition, the cytotoxicity of PIL was significantly reduced through hydrogen bond crosslinking. H4 showed the highest antibacterial activity and a good biocompatibility. The results indicated that the PVA&PIL hydrogels had great potential for wound dressing.

传统抑菌剂释放的抗生素或阴离子导致了细菌耐药性的产生和环境污染。离子液体(ILs)因其优异的物理、化学和生物特性,已成为抗菌剂的重要选择。本文评估了 1-乙烯基-3-丁基氯化咪唑([VBIM]Cl,IL)和聚(1-乙烯基-3-丁基氯化咪唑)(P[VBIM]Cl,PIL)的生物活性,并将潜在的抗菌材料用于合成水凝胶。使用菌落形成试验和牛津杯法测试了 IL 和 PIL 的抗菌效果。利用细胞计数-Kit-8(CCK-8)实验研究了 IL 的 IC50(半数最大抑制浓度)值,结果显示 IL 在 24 小时、48 小时和 72 小时的 IC50 值分别为 1.47 毫克/毫升、0.35 毫克/毫升和 0.33 毫克/毫升。在 24 小时、48 小时和 72 小时内,PIL 的 IC50 值分别为 12.15 微克/毫升、12.06 微克/毫升和 11.76 微克/毫升。PIL 与聚乙烯醇(PVA)进一步交联,通过冻融循环形成新型水凝胶。新制成的水凝胶具有较高的含水量、优异的吸水性和出色的机械性能。通过菌落形成试验和抑菌区试验,水凝胶表现出良好的抗菌效果(针对大肠杆菌和金黄色葡萄球菌),在液体培养基中培养 H4(由 0.5 克 PIL 和 1 克 PVA 合成)时,几乎 100%的细菌都被杀死。此外,通过氢键交联,PIL 的细胞毒性显著降低。H4 显示出最高的抗菌活性和良好的生物相容性。结果表明,PVA&PIL 水凝胶在伤口敷料方面具有很大的潜力。
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引用次数: 0
The effect of Silver addition and deformation parameters on mechanostructure, biodegradation, antimicrobial and mechanical properties of Zn-based biodegradable alloys. 银添加和变形参数对锌基生物降解合金的机械结构、生物降解、抗菌和机械性能的影响。
IF 2.3 4区 医学 Q3 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-01 Epub Date: 2024-07-30 DOI: 10.1177/08853282241268682
Bünyamin Aksakal, Sinan Sezek, Cevher Kürşat Macit

Although low mechanical properties, Zinc (Zn) alloy systems with Copper (Cu) and Silver (Ag) as alloying elements have strong biocompatibility and biodegradability characteristics. This study examined the effects of rolling parameters and Ag alloying on the mechanical, biodegradable, and final structure of an alloy based on Zn. Comparing treated and untreated specimens, the addition of Ag led to a considerable improvement in both hardness and compressive strength. The produced alloys with varying amounts of Ag (between 1 and 4 wt%) were cold rolled at 400-800 r/min and friction coefficients between 0.3 and 0.5. The alloys' ultimate strength rose with an increase in rolling speed for Zn1Cu4Ag, and hardness and compressive strengths rose to 80HV and 470 MPa, respectively. It was demonstrated that rolling force rose somewhat with Ag concentration but significantly increased with rolling speed and friction. E. Coli and S. aureus were used to assess the biodegradable alloys' antibacterial properties. For the Zn-1Cu-2Ag alloy, the inclusion of Ag resulted in a considerable (50%) rise in antibacterial activity that exceeded the effects seen in other alloy systems.

以铜(Cu)和银(Ag)为合金元素的锌(Zn)合金体系虽然机械性能较低,但具有很强的生物相容性和生物降解性。本研究考察了轧制参数和 Ag 合金对锌基合金的机械性能、生物降解性和最终结构的影响。将处理过的试样与未处理的试样进行比较,发现添加 Ag 后,硬度和抗压强度均有显著提高。在 400-800 r/min 的转速和 0.3-0.5 之间的摩擦系数条件下,对所生产的含不同量 Ag(1-4 wt%)的合金进行冷轧。Zn1Cu4Ag 的合金极限强度随着轧制速度的增加而提高,硬度和抗压强度分别达到 80HV 和 470 兆帕。研究表明,轧制力随银浓度的增加而增加,但随轧制速度和摩擦力的增加而显著增加。大肠杆菌和金黄色葡萄球菌被用来评估可生物降解合金的抗菌性能。就 Zn-1Cu-2Ag 合金而言,加入 Ag 后,抗菌活性显著提高(50%),超过了其他合金体系的抗菌效果。
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引用次数: 0
The mechanics of tissue-engineered temporomandibular joint discs: Current status and prospects for enhancement. 组织工程颞下颌关节盘的力学:现状与发展前景。
IF 2.3 4区 医学 Q3 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-01 Epub Date: 2024-07-18 DOI: 10.1177/08853282241265059
Yilin She, Yixin Sun, Nan Jiang

The temporomandibular joint (TMJ) disc is an essential protective but vulnerable fibrocartilage. Their high mechanical strength is vital in absorbing loads, reducing friction, and protecting the condylar surface. Many diseases can lead to the destruction or degeneration of the mechanical function of the TMJ disc. Unfortunately, conservative treatment is ineffective in restoring the defective mechanical properties of the discs. Tissue engineering has been investigated as a promising alternative treatment approach to approximate the properties of native tissue. However, it is difficult for tissue-engineered discs to obtain sufficient mechanical properties. Several approaches have been proposed to improve the mechanical properties of tissue-engineered constructs. In this review, we summarized the mechanical properties of native TMJ discs and discussed the current mechanical testing methods. We then summarized the current advances in improving the mechanical properties of TMJ disc tissue-engineered constructs. Moreover, existing challenges and outbreak directions are discussed. This review assists future research in better understanding the mechanical properties of both native and tissue-engineered TMJ discs. It provides new insights into future mechanical property enhancement for TMJ disc tissue engineering.

颞下颌关节(TMJ)椎间盘是一种重要的保护性纤维软骨,但也很脆弱。它们的高机械强度对于吸收负荷、减少摩擦和保护髁突表面至关重要。许多疾病都会导致颞下颌关节盘机械功能的破坏或退化。遗憾的是,保守治疗无法有效恢复椎间盘有缺陷的机械性能。组织工程是一种很有前景的替代治疗方法,它可以接近原生组织的特性。然而,组织工程椎间盘很难获得足够的机械性能。人们提出了几种方法来改善组织工程构建物的机械性能。在本综述中,我们总结了原生颞下颌关节盘的机械性能,并讨论了当前的机械测试方法。然后,我们总结了目前在改善颞下颌关节盘组织工程构建物机械性能方面的进展。此外,还讨论了现有的挑战和发展方向。本综述有助于未来的研究更好地了解原生颞下颌关节盘和组织工程颞下颌关节盘的机械性能。它为未来颞下颌关节盘组织工程的机械性能增强提供了新的见解。
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引用次数: 0
Preparation, characterization, and liver targeting evaluation of a novel sustained-release brucine self-assembled micelle mediated by glycyrrhetinic acid. 甘草亭酸介导的新型缓释布鲁宾自组装胶束的制备、表征和肝脏靶向评估
IF 2.3 4区 医学 Q3 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-01 Epub Date: 2024-07-20 DOI: 10.1177/08853282241258161
Qingxia Guan, Han Yang, Zhaorui Xia, Xiuyan Li, Yue Zhang, Zeyu Lin, Shaung Sun, Zhixin Yang, Xiaoying Zhou, Shaowa Lv, Yanhong Wang

Background: Cancer is a serious threat to human life, health and social development. In recent years, nanomicelles, as an emerging drug carrier material, have gradually entered people's field of vision because of their advantages of improving bioavailability, maintaining drug levels, reducing systemic side effects and increasing drug accumulation at target sites. Methods: In this study, B-GPSG nano-micelles were prepared by film dispersion hydration method using brucine as model drug and glycyrrhetinic acid-polyethylene glycol-3-methylene glycol-dithiodipropionic acid-glycerol monostearate polymer as nano-carrier. The preparation process, characterization, drug release in vitro, pharmacokinetics and liver targeting were investigated. Results: The results showed that the range of particle size, polydispersion index and Zeta potential were 102.7 ± 1.09 nm, 0.201 ± 0.02 and -24.5 ± 0.19 mV respectively. The entrapment efficiency and drug loading were 83.79 ± 2.13% and 12.56 ± 0.09%, respectively. The drug release experiments in vitro and pharmacokinetic experiments showed that it had obvious sustained release effect. For pharmacokinetics study, it shows that both the B-GPSG solution group and the B-PSG solution group changed the metabolic kinetic parameters of brucine, but the B-GPSG solution group had a better effect. Compared with the B-PSG solution group, the drug was more prolonged in rats. The half-life in the body and the retention time in the body of B-GPSG are more helpful to improve the bioavailability of the drug and play a long-term effect. The tail vein injection results of mice indicate that B-GPSG can target and accumulate brucine in the liver without affecting other key organs. Cell uptake experiments and tissue distribution experiments in vivo show that glycyrrhetinic acid modified nano-micelles can increase the accumulation of brucine in hepatocytes, has a good liver targeting effect, and can be used as a new preparation for the treatment of liver cancer. Conclusion: The B-SPSG prepared in this experiment can provide a new treatment method and research idea for the treatment of liver cancer.

背景:癌症严重威胁着人类的生命健康和社会发展。近年来,纳米细胞作为一种新兴的药物载体材料,因其具有提高生物利用度、维持药物浓度、减少全身副作用、增加药物在靶点蓄积等优点,逐渐进入人们的视野。研究方法本研究以布鲁新为模型药物,甘草亭酸-聚乙二醇-3-亚甲基乙二醇-二硫代二丙酸-单硬脂酸甘油酯聚合物为纳米载体,采用薄膜分散水合法制备了B-GPSG纳米微胶囊。对其制备过程、表征、体外药物释放、药代动力学和肝脏靶向性进行了研究。结果表明结果表明,粒度范围、多分散指数和 Zeta 电位分别为 102.7 ± 1.09 nm、0.201 ± 0.02 和 -24.5 ± 0.19 mV。药物包埋效率和载药量分别为 83.79 ± 2.13% 和 12.56 ± 0.09%。体外药物释放实验和药代动力学实验表明,它具有明显的缓释效果。药代动力学研究表明,B-GPSG 溶液组和 B-PSG 溶液组均改变了布鲁宾的代谢动力学参数,但 B-GPSG 溶液组的效果更好。与 B-PSG 溶液组相比,该药物在大鼠体内的作用时间更长。B-GPSG 在体内的半衰期和在体内的滞留时间更有利于提高药物的生物利用度,发挥长效作用。小鼠尾静脉注射结果表明,B-GPSG 能在肝脏中靶向蓄积布鲁氨酸,而不影响其他关键器官。体内细胞摄取实验和组织分布实验表明,甘草亭酸修饰的纳米微球可增加布鲁氨酸在肝细胞中的蓄积,具有良好的肝脏靶向作用,可作为治疗肝癌的新制剂。结论本实验制备的 B-SPSG 可为肝癌的治疗提供一种新的治疗方法和研究思路。
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引用次数: 0
Sodium alginate hydrogel encapsulating microglia cell lysate subjected to serum starvation for mitigating glioma cells. 海藻酸钠水凝胶包裹血清饥饿状态下的小胶质细胞裂解液,用于缓解胶质瘤细胞的生长。
IF 2.3 4区 医学 Q3 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-01 Epub Date: 2024-07-29 DOI: 10.1177/08853282241268694
Shenzhong Luo, Jilong Wang, Meng Gao

Glioma is the most common malignant tumor in the brain, accounting for over 80% of all primary intracranial tumors. The current clinical treatment has shown certain limitations. Although M1 type microglia can secrete various pro-inflammatory cytokines and are expected to be used for glioma treatment, direct use of microglia may lead to overactivation and trigger immune storms. Therefore, we first found that serum starvation can stimulate the transformation of microglia into M1 type. Subsequently, we found through comparative experiments that the inhibitory effect of microglial cell lysis medium on glioma cells was stronger than that of microglial cell culture medium. Finally, we successfully prepared sodium alginate hydrogel loaded with microglia lysis solution to achieve sustained inhibitory effect on the growth of glioma and avoid its proliferation.

胶质瘤是脑部最常见的恶性肿瘤,占颅内原发性肿瘤的80%以上。目前的临床治疗存在一定的局限性。虽然 M1 型小胶质细胞能分泌多种促炎细胞因子,有望用于胶质瘤治疗,但直接使用小胶质细胞可能导致过度激活,引发免疫风暴。因此,我们首先发现血清饥饿能刺激小胶质细胞转化为 M1 型。随后,我们通过对比实验发现,小胶质细胞裂解液对胶质瘤细胞的抑制作用强于小胶质细胞培养液。最后,我们成功制备出了负载有小胶质细胞溶解液的海藻酸钠水凝胶,实现了对胶质瘤生长的持续抑制作用,避免了胶质瘤的增殖。
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引用次数: 0
Copper ion-doped multifunctional hydrogel with mild photothermal enhancement promotes vascularized bone regeneration. 掺杂铜离子的多功能水凝胶具有温和的光热增强功能,可促进血管化骨再生。
IF 2.3 4区 医学 Q3 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-01 Epub Date: 2024-07-26 DOI: 10.1177/08853282241268683
Chao Han, Dingsong Lu, Suoping Yang, Chong Liu, Feng Guo, Kai Zhang, Peng Li

The design and construction of a new and excellent synthetic graft is of great significance in the field of bone defect repair and reconstruction. In this study, a dopamine modified chitosan hydrogel doped with Cu ions with a mild photothermal effect was designed to provide a better microenvironment to advance the bone repair via promote the angiogenesis and osteogenesis. Characterizations showed the successful synthesis of the material while it also presented excellent biocompatibility and mild photothermal effect under the irradiation of near-infrared light. Further, it could enhance the angiogenesis of HUVECs cells through promoting the ability of migration and tube formation and enhance the osteogenic differentiation of MC3T3-E1 cells via increasing the content of vital osteogenic factors including Runx2, Col-1, OPN, OCN, OSX, etc. The in vivo experiment also testified that it could promote the bone defect repair in rat models. These results indicate the multifunctional hydrogel is an ideal material for the treatment of bone defects and has good clinical application potential.

在骨缺损修复和重建领域,设计和构建一种新型、优良的合成移植物具有重要意义。本研究设计了一种掺杂铜离子的多巴胺修饰壳聚糖水凝胶,具有温和的光热效应,可通过促进血管生成和骨生成为骨修复提供更好的微环境。表征结果表明,该材料的合成非常成功,而且具有良好的生物相容性,在近红外线照射下具有温和的光热效应。此外,它还能通过促进迁移和管形成能力来增强 HUVECs 细胞的血管生成,并通过增加 Runx2、Col-1、OPN、OCN、OSX 等重要成骨因子的含量来增强 MC3T3-E1 细胞的成骨分化。体内实验也证明,它能促进大鼠模型的骨缺损修复。这些结果表明,多功能水凝胶是治疗骨缺损的理想材料,具有良好的临床应用潜力。
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引用次数: 0
Application of self-assembled antibacterial nanofiber loaded oriented artificial skin in infected diabetic-related wound regeneration 自组装抗菌纳米纤维负载定向人造皮肤在糖尿病相关感染伤口再生中的应用
IF 2.9 4区 医学 Q3 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-14 DOI: 10.1177/08853282241270963
Jie Yang, Shengyun Li
Diabetic patients develop wounds that exhibit delayed healing, prolonged inflammatory responses, and slower epithelialization kinetics compared to non-diabetic patients. Diabetic foot ulcers(DFUs) affect approximately 18.6 million people worldwide. The presence of a high glucose microenvironment in DFUs results in the significant accumulation of bacterial infection and advanced glycation end products (AGEs). To solve this, a self-assemble antibacterial nanofiber(ANF) loaded oriential artificial skin (ANF@OAS) was introduced in this research, which is consisted of L/D-phenylalanine derivatives coupled the natural antimicrobial peptides.The ANF@OAS can effectively reduce AGEs production and suppress multiple resistant bacteria. Additionally, the ANF@OAS can suppress infection and stimulate wound healing in infected diabetic mice.
与非糖尿病患者相比,糖尿病患者的伤口愈合延迟、炎症反应持续时间长、上皮化速度慢。全球约有 1,860 万人患有糖尿病足溃疡。为了解决这一问题,本研究推出了一种自组装抗菌纳米纤维(ANF)负载的天然人工皮肤(ANF@OAS),它由L/D-苯丙氨酸衍生物和天然抗菌肽组成。此外,ANF@OAS 还能抑制感染,刺激受感染的糖尿病小鼠伤口愈合。
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引用次数: 0
Preparation and osteogenesis of a multiple crosslinking silk fibroin/carboxymethyl chitosan/sodium alginate composite scaffold loading with mesoporous silica/poly (lactic acid-glycolic acid) microspheres. 负载介孔二氧化硅/聚(乳酸-乙醇酸)微球的多重交联蚕丝纤维素/羧甲基壳聚糖/海藻酸钠复合支架的制备与成骨。
IF 2.9 4区 医学 Q3 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-12 DOI: 10.1177/08853282241281439
Yiwan Shi,Zhaozhen Wang,Weikang Xu,Xiaolu Yu,Botao Gao,Xinting Zhou,Jiwen Chen,Kunfeng Jia,Lek Hang Cheang,Man Seng Tam,Huajun Wang,Xiaofei Zheng,Tingting Wu
Large bone defect repair is a striking challenge in orthopedics. Currently, inorganic-organic composite scaffolds are considered as a promising approach to these bone regeneration. Silicon ions (Si4+) are bioactive and beneficial to bone regeneration and Si4+-containing inorganic mesoporous silica (MS) can effectively load drugs for bone repair. To better control the release of drug, we prepared biodegradable MS/PLGA (MP) microspheres. MP loaded organic silk fibroin/carboxymethyl chitosan/sodium alginate (MP/SF/CMCS/SA) composite scaffolds were further constructed by genipin and Ca2+ crosslinking. All MP/SF/CMCS/SA scaffolds had good swelling ability, degradation rate and high porosity. The incorporation of 1% MP significantly enhanced the compressive strength of composite scaffolds. Besides, MP loaded scaffold showed a sustained release of Si4+ and Ca2+. Moreover, the release rate of rhodamine (a model drug) of MP/SF/CMCS/SA scaffolds was obviously lower than that of MP. When culturing with rat bone marrow mesenchymal stem cells, scaffolds with 1% MP displayed good proliferation, adhesion and enhanced osteogenic differentiation ability. Based on the results above, the addition of 1% MP in SF/CMCS/SA scaffolds is a prospective way for drug release in bone regeneration and is promising for further in vivo bone repair applications.
大面积骨缺损修复是整形外科面临的一项严峻挑战。目前,无机-有机复合支架被认为是一种很有前景的骨再生方法。硅离子(Si4+)具有生物活性,有利于骨再生,而含 Si4+ 的无机介孔二氧化硅(MS)能有效负载药物,用于骨修复。为了更好地控制药物释放,我们制备了可生物降解的 MS/PLGA (MP)微球。通过基因素和 Ca2+ 交联,进一步构建了负载 MP 的有机丝纤维素/羧甲基壳聚糖/海藻酸钠(MP/SF/CMCS/SA)复合支架。所有 MP/SF/CMCS/SA 支架都具有良好的膨胀能力、降解率和高孔隙率。1% 的 MP 能显著提高复合材料支架的抗压强度。此外,负载 MP 的支架显示出 Si4+ 和 Ca2+ 的持续释放。此外,MP/SF/CMCS/SA 支架的罗丹明(一种模型药物)释放率明显低于 MP。在培养大鼠骨髓间充质干细胞时,含有 1%MP的支架显示出良好的增殖、粘附和成骨分化能力。基于上述结果,在 SF/CMCS/SA 支架中添加 1%的 MP 是骨再生中药物释放的一种前瞻性方法,有望进一步应用于体内骨修复。
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引用次数: 0
Biomimetic synthetic test system based on hydroxyapatite cement for adhesive strength evaluation of experimental mineral-organic bone adhesive materials 基于羟基磷灰石骨水泥的仿生合成测试系统,用于评估矿物-有机骨粘合剂实验材料的粘合强度
IF 2.9 4区 医学 Q3 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-10 DOI: 10.1177/08853282241283537
Paul Frederik Otto, Sebastian Hienz, Silvia Mittmann, Niklas Dümmler, Tobias Renner, Csaba Gergely, Juliane Carolin Kade, Uwe Gbureck
The development of bone adhesive materials is a research field of high relevance for the advancement of clinical procedures. Despite this, there are currently no material candidates meeting the full range of requirements placed on such a material, such as biocompatibility, sufficient mechanical properties and bond strength under biological conditions, practical applicability in a clinical setting, and no adverse effect on the healing process itself. A serious obstacle to the advancement of the field is a lack in standardized methodology leading to comparable results between experiments and different research groups. Natural bone samples are the current gold-standard material used to perform adhesive strength experiments, however they come with a number of drawbacks, including high sample variability due to unavoidable natural causes and the impossibility to reliably recreate test conditions to repeat experiments. This paper introduces a valuable auxiliary test method capable of producing large numbers of synthetic test specimens which are chemically similar to bone and can be produced in different laboratories so to repeat experiments under constant conditions across laboratories. The substrate is based on a hydroxyapatite forming cement with addition of gelatine as organic component. Crosslinking of the organic component is performed to improve mechanical properties. In order to demonstrate the performance of the developed method, various experimental and commercial bone/tissue adhesive materials were tested and compared with results obtained by established methods to highlight the potential of the test system.
骨粘合材料的开发是一个与临床手术进展密切相关的研究领域。尽管如此,目前还没有候选材料能满足对此类材料的全部要求,如生物相容性、生物条件下足够的机械性能和粘接强度、临床实际应用性以及对愈合过程本身无不良影响等。该领域发展的一个严重障碍是缺乏标准化的方法,导致实验和不同研究小组之间的结果不具可比性。天然骨样本是目前用于粘合强度实验的黄金标准材料,但也存在一些缺点,包括由于不可避免的自然原因导致的样本高变异性,以及无法可靠地再现测试条件以重复实验。本文介绍了一种有价值的辅助测试方法,该方法能够制作大量与骨骼化学性质相似的合成测试样本,并可在不同实验室制作,从而在不同实验室的恒定条件下重复实验。基质以羟基磷灰石成型水泥为基础,添加明胶作为有机成分。对有机成分进行交联可提高机械性能。为了证明所开发方法的性能,对各种实验用和商用骨/组织粘合剂材料进行了测试,并将测试结果与既定方法得出的结果进行了比较,以突出测试系统的潜力。
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Journal of Biomaterials Applications
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