R. Jena, I. Y. Raja, V. Ramamoorthy, S. LAKSHMI NARAYANAN, R. Renuka, A. SUBBIAH, K. ERAIVAN ARUTKANI AIYANATHAN, V. KARTHIK PANDI, R. SIVADHARSHANAPRIYA
Grapevine powdery mildew is one of the most important plant diseases widely affecting crops in many countries. The main aim of the present study was to use Ampelomyces quisqualis isolates to suppress the powdery mildew of grapes under field conditions. The findings revealed a strong correlation between the mycoparasite, Ampelomyces quisqualis and the grape powdery mildew pathogens, suggesting its potential for effective control. The results revealed that a single spray of A. quisqualis @ 2.0 per cent (MDU1) succeeded by two sprays of Trichoderma asperellum @ 2.0 per cent (Tasp,7) were found to be effective against Erysiphe necator. In addition, liquid formulation of A. quisqualis isolate MDU1 (2% w/v) as a foliar spray at 2% containing 2 × 106 spores ml-1 the during initial occurrence of the disease up to three sprayings at 15 days interval was found to be best. Thus, a liquid formulation of A. quisqualis isolate MDU1 proved effective in managing grapes powdery mildew disease under field conditions. The rDNA ITS region of A. quisqualis isolates was analyzed at the molecular level, and the resulting sequences were subjected to GC-MS analysis. The secondary metabolite identification using GC-MS revealed the presence of antimicrobial compounds, including squalene with the highest peak of 4.643 percent, octadecanoic acid with 3.862 percent, tetradecanoic acid with 3.600 percent, and 9,12-octadecadienoic acid (Z,Z) with 1.451 percent. These bioactive compounds revealed by GC-MS analysis in crude extracts of A. quisqualis had a stronger antifungal activity against E. necator.
{"title":"Exploring eco-sensitive strategies for effective powdery mildew management in grapevines","authors":"R. Jena, I. Y. Raja, V. Ramamoorthy, S. LAKSHMI NARAYANAN, R. Renuka, A. SUBBIAH, K. ERAIVAN ARUTKANI AIYANATHAN, V. KARTHIK PANDI, R. SIVADHARSHANAPRIYA","doi":"10.18311/jbc/2023/34206","DOIUrl":"https://doi.org/10.18311/jbc/2023/34206","url":null,"abstract":"Grapevine powdery mildew is one of the most important plant diseases widely affecting crops in many countries. The main aim of the present study was to use Ampelomyces quisqualis isolates to suppress the powdery mildew of grapes under field conditions. The findings revealed a strong correlation between the mycoparasite, Ampelomyces quisqualis and the grape powdery mildew pathogens, suggesting its potential for effective control. The results revealed that a single spray of A. quisqualis @ 2.0 per cent (MDU1) succeeded by two sprays of Trichoderma asperellum @ 2.0 per cent (Tasp,7) were found to be effective against Erysiphe necator. In addition, liquid formulation of A. quisqualis isolate MDU1 (2% w/v) as a foliar spray at 2% containing 2 × 106 spores ml-1 the during initial occurrence of the disease up to three sprayings at 15 days interval was found to be best. Thus, a liquid formulation of A. quisqualis isolate MDU1 proved effective in managing grapes powdery mildew disease under field conditions. The rDNA ITS region of A. quisqualis isolates was analyzed at the molecular level, and the resulting sequences were subjected to GC-MS analysis. The secondary metabolite identification using GC-MS revealed the presence of antimicrobial compounds, including squalene with the highest peak of 4.643 percent, octadecanoic acid with 3.862 percent, tetradecanoic acid with 3.600 percent, and 9,12-octadecadienoic acid (Z,Z) with 1.451 percent. These bioactive compounds revealed by GC-MS analysis in crude extracts of A. quisqualis had a stronger antifungal activity against E. necator.","PeriodicalId":15188,"journal":{"name":"Journal of Biological Control","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86121099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
DANISHTA AZIZ, Wasim Yousuf, BARKAT HUSSAIN, ISHTIYAQ AHAD, ZAKIR HUSSAIN KHAN, SIVA KUMAR, T. VENKATESAN
Codling moth is being reported in other countries of the world. It is a destructive and direct pest on apples and other fruit crops. The pathway for the occurrence of codling moth in the isolated belt of Baramulla is a cause of concern, and there is a need to contain this insect pest in other fruit-growing areas of Kashmir Valley. Here, we report a codling moth attacking apple and pear plants in the incursion belt.The fruit damage on apple in the belt was recorded more than 10% on delicious varieties. The codling moth was monitored and identified by using species-specific sex pheromone. The morphological and molecular tools were used to identify the codling moth.
{"title":"Pheromone-based monitoring of codling moth in isolated belt of Baramulla, Jammu and Kashmir, India","authors":"DANISHTA AZIZ, Wasim Yousuf, BARKAT HUSSAIN, ISHTIYAQ AHAD, ZAKIR HUSSAIN KHAN, SIVA KUMAR, T. VENKATESAN","doi":"10.18311/jbc/2023/33663","DOIUrl":"https://doi.org/10.18311/jbc/2023/33663","url":null,"abstract":"Codling moth is being reported in other countries of the world. It is a destructive and direct pest on apples and other fruit crops. The pathway for the occurrence of codling moth in the isolated belt of Baramulla is a cause of concern, and there is a need to contain this insect pest in other fruit-growing areas of Kashmir Valley. Here, we report a codling moth attacking apple and pear plants in the incursion belt.The fruit damage on apple in the belt was recorded more than 10% on delicious varieties. The codling moth was monitored and identified by using species-specific sex pheromone. The morphological and molecular tools were used to identify the codling moth.","PeriodicalId":15188,"journal":{"name":"Journal of Biological Control","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88461247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. SWAPNA RANI, S. PAL, K. T. Shivakumara, Ankita Gupta
A preliminary study was conducted from 2021 to 2022 to explore the parasitoid fauna associated with insect pests and their per cent parasitism levels in the field as well as to understand the diversity of parasitoids in the medicinal plants ecosystem under northern tracts of West Bengal. In course of the study, a total of ten parasitoid species belonging to 5 families from 2 orders were observed in this ecosystem. Hymenopteran parasitoids were dominant, i.e., more than 90% and only one fly parasitoid species from Diptera belonging to the family Tachinidae was observed. Amongst the hymenopteran parasitoids, braconids were the prime species, found parasitizing various insect pests. All these parasitoids were observed attacking lepidopteran caterpillars which were predominant defoliators on various medicinal plants. The per cent parasitism by Copidosoma sp. on Helcystogramma hibisci was 38% which was the highest and found effective in the suppression of pest population.
{"title":"Diversity and abundance of parasitoid fauna associated with the pests of certain medicinal plants of West Bengal","authors":"K. SWAPNA RANI, S. PAL, K. T. Shivakumara, Ankita Gupta","doi":"10.18311/jbc/2023/33841","DOIUrl":"https://doi.org/10.18311/jbc/2023/33841","url":null,"abstract":"A preliminary study was conducted from 2021 to 2022 to explore the parasitoid fauna associated with insect pests and their per cent parasitism levels in the field as well as to understand the diversity of parasitoids in the medicinal plants ecosystem under northern tracts of West Bengal. In course of the study, a total of ten parasitoid species belonging to 5 families from 2 orders were observed in this ecosystem. Hymenopteran parasitoids were dominant, i.e., more than 90% and only one fly parasitoid species from Diptera belonging to the family Tachinidae was observed. Amongst the hymenopteran parasitoids, braconids were the prime species, found parasitizing various insect pests. All these parasitoids were observed attacking lepidopteran caterpillars which were predominant defoliators on various medicinal plants. The per cent parasitism by Copidosoma sp. on Helcystogramma hibisci was 38% which was the highest and found effective in the suppression of pest population.","PeriodicalId":15188,"journal":{"name":"Journal of Biological Control","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73363366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Ruqiya, H. C. Girisha, C. Manjunatha, R. Rangeshwaran, A. Kandan, G. Sivakumar, M. K. Prasannakumar, D. Pramesh, K. T. Shivakumara, H. S. Venu, S. Nanditha, K. S. Ankitha, K. Aditya, N. Aarthi, S. N. Sushil
Bacillus subtilis is a Gram-positive and endospore producing bacterium. Limited studies have shown that lipopeptides produced by B. subtilis can be inhibitory to phytopathogens. Sclerotinia sclerotiorum is a plant pathogenic fungus which causes various diseases like cotton rot, watery soft rot, stem rot, crown rot and blossom blight in vegetable crops. The objective of the study was to isolate lipopeptides from B. subtilis and study their inhibitory potential against S. sclerotiorum. So, the B. subtilis isolates were extracted from the collected soils of Western Ghats of India. They were initially characterized through morphological parameters followed by PCR amplification of the 16S rDNA gene and confirmation through BLAST algorithm in NCBI database. The lipopeptides produced by these isolates were tested against S. sclerotiorum. B. subtilis strains were effective against S. sclerotiorum and exhibited 18.33 to 29.5 % inhibition under dual culture bio-assay. The antagonistic activity of lipopeptides extracted from B. subtilis strains showed 21.56 to 88.89 % inhibition of S. sclerotiorum in the lowest to highest concentration of lipopeptide tested and was found to be significantly higher than the control. The present study has shown that B. subtilis strains vary in the production of lipopeptides and some of them could produce lipopeptides that are highly inhibitory to S. sclerotiorum. B. subtilis strain NBAIR BSWG1 showed the highest inhibition for S. sclerotiorum. Lipopeptide based poison food technique and the dual culture bioassay results showed that B. subtilis strain NBAIR BSWG1 has immense potential for use in the biological control of S. sclerotiorum. Further studies are being carried out in formulating the lipopeptides for field application.
{"title":"Biocontrol potential and molecular characterization of lipopeptides producing Bacillus subtilis against Sclerotinia sclerotiorum","authors":"S. Ruqiya, H. C. Girisha, C. Manjunatha, R. Rangeshwaran, A. Kandan, G. Sivakumar, M. K. Prasannakumar, D. Pramesh, K. T. Shivakumara, H. S. Venu, S. Nanditha, K. S. Ankitha, K. Aditya, N. Aarthi, S. N. Sushil","doi":"10.18311/jbc/2022/33785","DOIUrl":"https://doi.org/10.18311/jbc/2022/33785","url":null,"abstract":"Bacillus subtilis is a Gram-positive and endospore producing bacterium. Limited studies have shown that lipopeptides produced by B. subtilis can be inhibitory to phytopathogens. Sclerotinia sclerotiorum is a plant pathogenic fungus which causes various diseases like cotton rot, watery soft rot, stem rot, crown rot and blossom blight in vegetable crops. The objective of the study was to isolate lipopeptides from B. subtilis and study their inhibitory potential against S. sclerotiorum. So, the B. subtilis isolates were extracted from the collected soils of Western Ghats of India. They were initially characterized through morphological parameters followed by PCR amplification of the 16S rDNA gene and confirmation through BLAST algorithm in NCBI database. The lipopeptides produced by these isolates were tested against S. sclerotiorum. B. subtilis strains were effective against S. sclerotiorum and exhibited 18.33 to 29.5 % inhibition under dual culture bio-assay. The antagonistic activity of lipopeptides extracted from B. subtilis strains showed 21.56 to 88.89 % inhibition of S. sclerotiorum in the lowest to highest concentration of lipopeptide tested and was found to be significantly higher than the control. The present study has shown that B. subtilis strains vary in the production of lipopeptides and some of them could produce lipopeptides that are highly inhibitory to S. sclerotiorum. B. subtilis strain NBAIR BSWG1 showed the highest inhibition for S. sclerotiorum. Lipopeptide based poison food technique and the dual culture bioassay results showed that B. subtilis strain NBAIR BSWG1 has immense potential for use in the biological control of S. sclerotiorum. Further studies are being carried out in formulating the lipopeptides for field application.","PeriodicalId":15188,"journal":{"name":"Journal of Biological Control","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73405624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abissi Marc Adjaoke, Ghislain Tepa Yotto, A. Adandonon
Foods derived from plants are important sources of food for natural enemies and influence several functions of their existence, including reproduction. The objective of the current study was to assess the effect of different sources of food and host densities on the oviposition efficiency of key parasitoids of Spodoptera frugiperda. The experiment was set up under laboratory conditions in a completely randomized block design with three factors namely parasitoids (four), feeding (groundnut pollen, soybean nectar, honey, starved as control) and host densities (five), a total of 80 treatments. There were five replications for the experimental design while the experiment was repeated four times, a total of 20 replications for each treatment. Results showed that the functional response of adult parasitoids was influenced both by food sources and host densities. Soybean nectar worked the best like honey, followed by groundnut pollen. Soybeans and groundnut may therefore have agroecological interests beneficial for mass rearing to promote key parasitoids as biological agents to control S. frugiperda. As for the host densities, the minimum and maximum of the parasitized host were obtained respectively with the densities of 20 and 100 eggs per female for T. remus, 20 and 80 eggs for Trichogramma sp. and C. insularis and 5 to 50 larvae for C. marginiventris. Taking into account the interesting results obtained which would be favourable to the rapid multiplication of parasitoids, tests in real environments are necessary to test the influence of the main environmental factors on the performance of said parasitoids.
{"title":"Diet source-dependent functional response of key parasitoids of Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera, Noctuidae)","authors":"Abissi Marc Adjaoke, Ghislain Tepa Yotto, A. Adandonon","doi":"10.18311/jbc/2022/30873","DOIUrl":"https://doi.org/10.18311/jbc/2022/30873","url":null,"abstract":"Foods derived from plants are important sources of food for natural enemies and influence several functions of their existence, including reproduction. The objective of the current study was to assess the effect of different sources of food and host densities on the oviposition efficiency of key parasitoids of Spodoptera frugiperda. The experiment was set up under laboratory conditions in a completely randomized block design with three factors namely parasitoids (four), feeding (groundnut pollen, soybean nectar, honey, starved as control) and host densities (five), a total of 80 treatments. There were five replications for the experimental design while the experiment was repeated four times, a total of 20 replications for each treatment. Results showed that the functional response of adult parasitoids was influenced both by food sources and host densities. Soybean nectar worked the best like honey, followed by groundnut pollen. Soybeans and groundnut may therefore have agroecological interests beneficial for mass rearing to promote key parasitoids as biological agents to control S. frugiperda. As for the host densities, the minimum and maximum of the parasitized host were obtained respectively with the densities of 20 and 100 eggs per female for T. remus, 20 and 80 eggs for Trichogramma sp. and C. insularis and 5 to 50 larvae for C. marginiventris. Taking into account the interesting results obtained which would be favourable to the rapid multiplication of parasitoids, tests in real environments are necessary to test the influence of the main environmental factors on the performance of said parasitoids.","PeriodicalId":15188,"journal":{"name":"Journal of Biological Control","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88349782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Senthil Kumar, S. Jayakumar, Dhurairaj Satheesh
Panchagavya is an organic formulation, formed by merging five cow derivatives, such as dung, urine, milk, ghee, and curd, which promotes the growth of plants. The present investigation was aimed to determine the larvicidal efficacy of Panchagavya with varied concentrations against major pests of teak, Tectona grandis, singly, and combined with leaf extract of members of Meliaceae family, and coupled with extract of seaweed, in experimental research plots. Observations on occurrence of pests was also observed periodically and recorded. Treatments include 5% concentration of panchagavya (T1), which serves as a control and Panchagavya combined with varied dosage of other leaf crude extracts of Melia dubia (T2), Melia azedarach (T3), Azadirachta indica (T4), and a seaweed, Sargassum wightii (T5), with a concentration range of 1000-5000 ppm that were used as foliar application. Among the various pests, Hyblaea puera, and Eutectona machaeralis were found to be dominant, and the III instar larval stage were targeted for the management. Maximum larval mortality was observed with exposure to Panchagavya with crude extract of S. wightii, at an appropriate ratio (T5) against teak defoliator, Hyblaea puera. The per cent pest reduction was found to be 65% and 73% in 48 h and 72 h time interval. Similarly, higher level of larval mortality was recorded against skeletonizer (E. machaeralis) where the reduction per cent was recorded as 62% and 71% at 5000 ppm in (T5) within the interval of 48 h and 72 h. Consequently, a distinct individual lethal effect of Panchagavya (T1, control) against both pests was found to be 8%, 24%, and 46% with similar time frames. However, the efficacy of Panchagavya combined with extract of M. dubia, M. azedarach and A. indica exhibited statistically significant ability in larvicidal potential against H. puera. Whereas, treatments like T2, T3, and T4 showed 59, 67, and 62 % of larval mortality of E. machaeralis larva within the period of 72 h. The results of the study affirmed that the synergistic effects of Panchagavya coupled with seaweed extract at appropriate ratio proved to be effective against major pests of teak plantation.
{"title":"Evaluation of larvicidal activity of Panchagavya, against the teak defoliator (Hyblaea puera Cramer) and skeletonizer (Eutectona machaeralis Walker) in a forest plantation","authors":"M. Senthil Kumar, S. Jayakumar, Dhurairaj Satheesh","doi":"10.18311/jbc/2022/31732","DOIUrl":"https://doi.org/10.18311/jbc/2022/31732","url":null,"abstract":"Panchagavya is an organic formulation, formed by merging five cow derivatives, such as dung, urine, milk, ghee, and curd, which promotes the growth of plants. The present investigation was aimed to determine the larvicidal efficacy of Panchagavya with varied concentrations against major pests of teak, Tectona grandis, singly, and combined with leaf extract of members of Meliaceae family, and coupled with extract of seaweed, in experimental research plots. Observations on occurrence of pests was also observed periodically and recorded. Treatments include 5% concentration of panchagavya (T1), which serves as a control and Panchagavya combined with varied dosage of other leaf crude extracts of Melia dubia (T2), Melia azedarach (T3), Azadirachta indica (T4), and a seaweed, Sargassum wightii (T5), with a concentration range of 1000-5000 ppm that were used as foliar application. Among the various pests, Hyblaea puera, and Eutectona machaeralis were found to be dominant, and the III instar larval stage were targeted for the management. Maximum larval mortality was observed with exposure to Panchagavya with crude extract of S. wightii, at an appropriate ratio (T5) against teak defoliator, Hyblaea puera. The per cent pest reduction was found to be 65% and 73% in 48 h and 72 h time interval. Similarly, higher level of larval mortality was recorded against skeletonizer (E. machaeralis) where the reduction per cent was recorded as 62% and 71% at 5000 ppm in (T5) within the interval of 48 h and 72 h. Consequently, a distinct individual lethal effect of Panchagavya (T1, control) against both pests was found to be 8%, 24%, and 46% with similar time frames. However, the efficacy of Panchagavya combined with extract of M. dubia, M. azedarach and A. indica exhibited statistically significant ability in larvicidal potential against H. puera. Whereas, treatments like T2, T3, and T4 showed 59, 67, and 62 % of larval mortality of E. machaeralis larva within the period of 72 h. The results of the study affirmed that the synergistic effects of Panchagavya coupled with seaweed extract at appropriate ratio proved to be effective against major pests of teak plantation.","PeriodicalId":15188,"journal":{"name":"Journal of Biological Control","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90777943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chickpea (Cicer arietinum L.) is grown in more than 50 countries. India is the largest chickpea-producing country accounting for 64% of the global chickpea production. However, the production is contrained by the dry root rot disease caused by Rhizoctonia bataticola. Considering this problem, the investigation was carried out to isolate, characterize and the antagonistic potential of indigenous endophytic PGPMs for one of the components in the integrated management of dry root rot of chickpeas in eco-friendly manner. Hence, the isolation of thirty endophytic PGPMs was carried from chickpea by using the spread plate technique. The cultural characters and Gram’s staining reaction confirmed that the endophytic PGPMs isolated from chickpea plant tissues were bacteria. Among thirty bacterial strains, eight showed more than 50% of mycelial inhibition of the pathogen. Out of eight strains, five highly superior strains were selected and subjected for 16S rDNA gene sequencing using the universal primers (16Sr DNA F and 16Sr DNA R), which produced amplified products of size 1500 bp. nBLAST results of 16S rDNA gene sequence revealed that all the endophytic bacterial PGPMs showed homology with genus Bacillus but with different species. The five potential strains namely, BEPGPM-5, BEPGPM-9, BEPGPM-27, BEPGPM-28, and BEPGPM-30 were identified and confirmed as B. tropicus, B. pacificus, B. cereus, B. subtilis, respectively, based on molecular technique.
鹰嘴豆(Cicer arietinum L.)在50多个国家种植。印度是最大的鹰嘴豆生产国，占全球鹰嘴豆产量的64%。然而，由于bataticola根核菌引起的干腐病，生产受到制约。针对这一问题，本研究对鹰嘴豆干根腐病生态综合治理中本土内生植物PGPMs的分离、鉴定及其拮抗潜力进行了研究。为此，采用平板扩散技术从鹰嘴豆中分离了30株内生PGPMs。培养特性和革兰氏染色反应证实从鹰嘴豆植物组织中分离到的内生PGPMs为细菌。在30株细菌中，有8株对病原菌的菌丝抑制率超过50%。从8株菌株中选择5株高度优良的菌株，使用通用引物(16Sr DNA F和16Sr DNA R)进行16S rDNA基因测序，扩增产物大小为1500bp。16S rDNA基因序列nBLAST分析结果显示，所有内生细菌PGPMs均与芽孢杆菌属同源，但属不同。利用分子技术鉴定出5株潜在菌株BEPGPM-5、BEPGPM-9、BEPGPM-27、BEPGPM-28和BEPGPM-30分别为热带芽孢杆菌、太平洋芽孢杆菌、蜡样芽孢杆菌和枯草芽孢杆菌。
{"title":"Indigenous bacterial endophytic PGPMs of chickpea: Characterization and hidden antagonistic potential against Rhizoctonia bataticola causing dry root rot of chickpea","authors":"G. Sunkad, Meghana S. Patil, Ranjana Joshi","doi":"10.18311/jbc/2022/32473","DOIUrl":"https://doi.org/10.18311/jbc/2022/32473","url":null,"abstract":"Chickpea (Cicer arietinum L.) is grown in more than 50 countries. India is the largest chickpea-producing country accounting for 64% of the global chickpea production. However, the production is contrained by the dry root rot disease caused by Rhizoctonia bataticola. Considering this problem, the investigation was carried out to isolate, characterize and the antagonistic potential of indigenous endophytic PGPMs for one of the components in the integrated management of dry root rot of chickpeas in eco-friendly manner. Hence, the isolation of thirty endophytic PGPMs was carried from chickpea by using the spread plate technique. The cultural characters and Gram’s staining reaction confirmed that the endophytic PGPMs isolated from chickpea plant tissues were bacteria. Among thirty bacterial strains, eight showed more than 50% of mycelial inhibition of the pathogen. Out of eight strains, five highly superior strains were selected and subjected for 16S rDNA gene sequencing using the universal primers (16Sr DNA F and 16Sr DNA R), which produced amplified products of size 1500 bp. nBLAST results of 16S rDNA gene sequence revealed that all the endophytic bacterial PGPMs showed homology with genus Bacillus but with different species. The five potential strains namely, BEPGPM-5, BEPGPM-9, BEPGPM-27, BEPGPM-28, and BEPGPM-30 were identified and confirmed as B. tropicus, B. pacificus, B. cereus, B. subtilis, respectively, based on molecular technique.","PeriodicalId":15188,"journal":{"name":"Journal of Biological Control","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89054839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The importance of augmentative biological control and chemical control, and the need and scope for integrating these, rather than dismissing them as incompatible, so as to create a win-win situation for both are highlighted. Besides the judicious use of chemicals and periodical releases of parasitoids/predators, other options such as insect-resistant transgenic crops, host plant resistance, botanical insecticides, sex pheromones, trap crops, pest resistance management, new product development, regulatory measures, etc., should also be exploited, as appropriate to a given situation, so as to develop an Integrated Pest Management (IPM) package. The practicality of such an IPM is explained, citing Bt cotton as an example. It is reiterated that IPM is the most prudent approach for sustainable crop production and protection with the major emphasis being laid on biological control and other eco-friendly methods as indicated by the latest global trend.
{"title":"Integration of augmentative biocontrol with synthetic pesticides and other control methods for IPM – Challenges and prospects*","authors":"T. Manjunath","doi":"10.18311/jbc/2022/34091","DOIUrl":"https://doi.org/10.18311/jbc/2022/34091","url":null,"abstract":"The importance of augmentative biological control and chemical control, and the need and scope for integrating these, rather than dismissing them as incompatible, so as to create a win-win situation for both are highlighted. Besides the judicious use of chemicals and periodical releases of parasitoids/predators, other options such as insect-resistant transgenic crops, host plant resistance, botanical insecticides, sex pheromones, trap crops, pest resistance management, new product development, regulatory measures, etc., should also be exploited, as appropriate to a given situation, so as to develop an Integrated Pest Management (IPM) package. The practicality of such an IPM is explained, citing Bt cotton as an example. It is reiterated that IPM is the most prudent approach for sustainable crop production and protection with the major emphasis being laid on biological control and other eco-friendly methods as indicated by the latest global trend.","PeriodicalId":15188,"journal":{"name":"Journal of Biological Control","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85776902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. B. Akshaya, A. S. Krishnamoorthy, S. Nakkeeran, U. Sivakumar, G. Thiribhuvanamala
Plant diseases especially wilt disease caused by Fusarium spp. pose a major threat to the cultivation of vegetables. In the present study, experiments were undertaken to explore the potential antifungal metabolites produced by macro basidiomycetes viz., Lentinus edodes, Ganoderma lucidum and Schizophyllum commune against Fusarium oxysporum and F. solani causing wilt disease of cucumber and capsicum. Among these, the ethyl acetate fraction of Cell-Free Culture Filtrate (CFC) of L. edodes exhibited maximum per cent inhibition of the mycelial growth of F. oxysporum and F. solani (61.11 and 57.77 %, respectively) at a concentration of 2000 ppm. Characterization of antifungal metabolites of Cell Free Condensate (CFC) of ethyl acetate fraction of L. edodes observed as prominent bands in Thin Layer Chromatography (TLC) indicated with an RF value of 0.25 and 0.69. Further GC-MS characterization of TLC-eluted compounds from L. edodes indicated the presence of 14 different compounds including 2H-pyran-2-one 6-pentyl-, possessing antifungal activity. The Fouriertransform Infrared Spectroscopy (FTIR) spectrum revealed the functional groups such as alcohol (O-H), amides (C-O), aliphatic polyes (CH2), triazenes (N=N), silicon compounds (Si-O-Si), amines (C-N) and phosphorus (P=S). The comparison of metabolite distribution patterns by Principal Component Analysis (PCA) obtained from L. edodes (PC 1) showed a positive correlation between the compounds. This study infers that L. edodes possess antifungal activity against F. oxysporum and F. solani that can be explored for formulation and application of these antifungal compounds in plant protection.
{"title":"Harnessing antifungal metabolites from macro basidiomycetes against wilt inciting Fusarium spp.","authors":"S. B. Akshaya, A. S. Krishnamoorthy, S. Nakkeeran, U. Sivakumar, G. Thiribhuvanamala","doi":"10.18311/jbc/2022/31880","DOIUrl":"https://doi.org/10.18311/jbc/2022/31880","url":null,"abstract":"Plant diseases especially wilt disease caused by Fusarium spp. pose a major threat to the cultivation of vegetables. In the present study, experiments were undertaken to explore the potential antifungal metabolites produced by macro basidiomycetes viz., Lentinus edodes, Ganoderma lucidum and Schizophyllum commune against Fusarium oxysporum and F. solani causing wilt disease of cucumber and capsicum. Among these, the ethyl acetate fraction of Cell-Free Culture Filtrate (CFC) of L. edodes exhibited maximum per cent inhibition of the mycelial growth of F. oxysporum and F. solani (61.11 and 57.77 %, respectively) at a concentration of 2000 ppm. Characterization of antifungal metabolites of Cell Free Condensate (CFC) of ethyl acetate fraction of L. edodes observed as prominent bands in Thin Layer Chromatography (TLC) indicated with an RF value of 0.25 and 0.69. Further GC-MS characterization of TLC-eluted compounds from L. edodes indicated the presence of 14 different compounds including 2H-pyran-2-one 6-pentyl-, possessing antifungal activity. The Fouriertransform Infrared Spectroscopy (FTIR) spectrum revealed the functional groups such as alcohol (O-H), amides (C-O), aliphatic polyes (CH2), triazenes (N=N), silicon compounds (Si-O-Si), amines (C-N) and phosphorus (P=S). The comparison of metabolite distribution patterns by Principal Component Analysis (PCA) obtained from L. edodes (PC 1) showed a positive correlation between the compounds. This study infers that L. edodes possess antifungal activity against F. oxysporum and F. solani that can be explored for formulation and application of these antifungal compounds in plant protection.","PeriodicalId":15188,"journal":{"name":"Journal of Biological Control","volume":"179 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80111558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Soundara Rajan, T. Sivakumar, P. Balabaskar, R. Parthasarathy
Coffee is an important beverage crop in India. The soil-borne pathogen, Rhizoctonia solani Khun is known to cause collar rot of coffee seedlings in the nursery itself and accounts for 10-25% mortality under conducive environmental conditions. This paper presents the efficacy of various microbial antagonists used in the form of biocapsules for management of coffee collar rot. Among the various antagonists used, Bacillus subtilis resulted in the maximum reduction of collar rot incidence compared to Trichoderma harzianum and Pseudomonas fluorescens.
{"title":"Bioprospecting: An innovative technology for the management of coffee collar rot disease","authors":"S. Soundara Rajan, T. Sivakumar, P. Balabaskar, R. Parthasarathy","doi":"10.18311/jbc/2022/33792","DOIUrl":"https://doi.org/10.18311/jbc/2022/33792","url":null,"abstract":"Coffee is an important beverage crop in India. The soil-borne pathogen, Rhizoctonia solani Khun is known to cause collar rot of coffee seedlings in the nursery itself and accounts for 10-25% mortality under conducive environmental conditions. This paper presents the efficacy of various microbial antagonists used in the form of biocapsules for management of coffee collar rot. Among the various antagonists used, Bacillus subtilis resulted in the maximum reduction of collar rot incidence compared to Trichoderma harzianum and Pseudomonas fluorescens.","PeriodicalId":15188,"journal":{"name":"Journal of Biological Control","volume":"191 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76638193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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