Xiaoli Li, Xiaogang Wang, Weiye Liu, Wenqiang Li, Meifeng Li
This work aimed to evaluate the effectiveness of several anti-TLR4 nanobody administration techniques in a gram-negative bacterial sepsis (GNBS) rat model. The targeting proteins for TI-Nb2 and TC-Nb6 anti-TLR4 nanobodies were TLR4203-348 and TLR4349-582, respectively. The survival times (STs) of 44 Sprague-Dawley (SD) rats were tracked in the TI-Nb2, TC-Nb6, TI-Nb2+TC-Nb6, and D0 groups (saline control). Besides, the ELISA was utilized to measure the levels of TNF-, IL-1, IL-8, and IL-10 in different groups. An automatic biochemical analyzer was employed to determine the levels of AST, ALT, AMS, CRE, and Urea. Furthermore, the rat liver and kidney tissue samples were stained with hematoxylin-eosin (HE). Cleaved-caspase-3 (CC3) protein expression (PE) in rat tissues was discovered using immunohistochemistry, and the positive unit (PU) value was computed. The TI-Nb2+TC-Nb6 group exhibited a longder ST, lower TNF- α , IL-1 β , IL-8, ALT, AST, AMS, CRE, and Urea levels, and a smaller CC3 protein PU value in nucleus and cytoplasm than the TI-Nb2, TC-Nb6, and D0 groups (all P <0.05). The above findings indicated that the combined usage of TI-Nb2 and TC-Nb6 can successfully reduce the expression levels of CC3 protein, biochemical markers, and inflammatory factors. This could protect the liver, kidneys, and other organs and prolong the ST of sepsis rats.
{"title":"Analysis of the Pathogenesis of Gram-Negative Bacterial Sepsis in Rats Under Nano-Body","authors":"Xiaoli Li, Xiaogang Wang, Weiye Liu, Wenqiang Li, Meifeng Li","doi":"10.1166/jbn.2023.3653","DOIUrl":"https://doi.org/10.1166/jbn.2023.3653","url":null,"abstract":"This work aimed to evaluate the effectiveness of several anti-TLR4 nanobody administration techniques in a gram-negative bacterial sepsis (GNBS) rat model. The targeting proteins for TI-Nb2 and TC-Nb6 anti-TLR4 nanobodies were TLR4203-348 and TLR4349-582, respectively. The survival times (STs) of 44 Sprague-Dawley (SD) rats were tracked in the TI-Nb2, TC-Nb6, TI-Nb2+TC-Nb6, and D0 groups (saline control). Besides, the ELISA was utilized to measure the levels of TNF-, IL-1, IL-8, and IL-10 in different groups. An automatic biochemical analyzer was employed to determine the levels of AST, ALT, AMS, CRE, and Urea. Furthermore, the rat liver and kidney tissue samples were stained with hematoxylin-eosin (HE). Cleaved-caspase-3 (CC3) protein expression (PE) in rat tissues was discovered using immunohistochemistry, and the positive unit (PU) value was computed. The TI-Nb2+TC-Nb6 group exhibited a longder ST, lower TNF- α , IL-1 β , IL-8, ALT, AST, AMS, CRE, and Urea levels, and a smaller CC3 protein PU value in nucleus and cytoplasm than the TI-Nb2, TC-Nb6, and D0 groups (all P <0.05). The above findings indicated that the combined usage of TI-Nb2 and TC-Nb6 can successfully reduce the expression levels of CC3 protein, biochemical markers, and inflammatory factors. This could protect the liver, kidneys, and other organs and prolong the ST of sepsis rats.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The early metastasis of inflammatory M1 macrophages to M2 macrophages is an early marker for macrophages to play an anti-inflammatory role, while the role of macrophages in the kidney injury induced by sepsis is still poorly studied. We used septic serum to treat BMDMs at several time points, and then detect the expression of GCN2 in BMDMs. Western blot was used to detect the expression of iNos and Arg1 of macrophages. ELISA was used to detect the inflammatory cytokines. In vivo , the mice model of septic renal injury was established and immunohistochemistry was used to detect M1 and M2 markers, and IL-6 level. BUN, Scr and NAG were detected to assess renal function. The protein expression of GCN2 was increased in septic serum-stimulated BMDMs. WB results showed that GCN2 promote macrophage M1 to M2 polarization and decrease inflammation in vitro . GCN2 expression was increased in response to sepsis induced renal injury In vivo . When we overexpressed GCN2, there were more M1 polarizing to M2 and less inflammation, and it will improve renal function. Our study confirmed that increasing GCN2 expression can drive the polarization of M1 macrophages to M2, alleviate the renal inflammation and improve renal function induced by LPS.
{"title":"General Control Nonderepressible 2 Promotes M2 Macrophages Polarization and Renal Function After Sepsis-Induced Renal Injury","authors":"Hongfei Wang, Zhu Lin, Wenhua Li, Lin Dou","doi":"10.1166/jbn.2023.3624","DOIUrl":"https://doi.org/10.1166/jbn.2023.3624","url":null,"abstract":"The early metastasis of inflammatory M1 macrophages to M2 macrophages is an early marker for macrophages to play an anti-inflammatory role, while the role of macrophages in the kidney injury induced by sepsis is still poorly studied. We used septic serum to treat BMDMs at several time points, and then detect the expression of GCN2 in BMDMs. Western blot was used to detect the expression of iNos and Arg1 of macrophages. ELISA was used to detect the inflammatory cytokines. In vivo , the mice model of septic renal injury was established and immunohistochemistry was used to detect M1 and M2 markers, and IL-6 level. BUN, Scr and NAG were detected to assess renal function. The protein expression of GCN2 was increased in septic serum-stimulated BMDMs. WB results showed that GCN2 promote macrophage M1 to M2 polarization and decrease inflammation in vitro . GCN2 expression was increased in response to sepsis induced renal injury In vivo . When we overexpressed GCN2, there were more M1 polarizing to M2 and less inflammation, and it will improve renal function. Our study confirmed that increasing GCN2 expression can drive the polarization of M1 macrophages to M2, alleviate the renal inflammation and improve renal function induced by LPS.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"135 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ke He, Yanqi Wu, Yanting He, Jun Jin, Qing Hu, Jia Wu, Guangxun Cui, Jun Zhang, Yong Liu
With the continuous improvement of people’s requirements for the living environment, healthy and green living materials have been favored by the market. The development of nanotechnology provides a new direction for the research and development of healthy human settlement materials. Nano-microcapsules are used as carriers to prepare new building coating materials with efficient antibacterial agents, which can achieve slow release and efficient antibacterial properties. It has important application value for improving bacterial pollution on indoor walls and providing a clean-living environment. Silver nanoparticles coated with nano-microcapsules (AgNPs@PS-NMP) were prepared by one-step method for killing Staphylococcus aureus ( S. aureus ). SEM results showed that the particle size was about 500–600 nm, the particle size was uniform, the degree of agglomeration was small, and the morphology was complete. The Zeta potential was approximately −18.8 mV. The coating construction performance, hydrophilicity, drying time of AgNPs@PS-NMP were investigated respectively. The antibacterial activity of AgNPs@PS-NMP was further studied. The results showed that the nano-microcapsules had a good killing effect on S. aureus , and the minimum bactericidal concentration (MBC) was 28 μ g/mL. These results suggest that AgNPs@PS-NMP, as a new type of coating, not only has good coating properties but also excellent antibacterial properties. It can be used as a potential antibacterial building material for hospitals, schools and other places.
{"title":"Constructed Nano-Microcapsule Coating Coated with AgNPs for Enhanced Antibacterial Activity Effectively","authors":"Ke He, Yanqi Wu, Yanting He, Jun Jin, Qing Hu, Jia Wu, Guangxun Cui, Jun Zhang, Yong Liu","doi":"10.1166/jbn.2023.3600","DOIUrl":"https://doi.org/10.1166/jbn.2023.3600","url":null,"abstract":"With the continuous improvement of people’s requirements for the living environment, healthy and green living materials have been favored by the market. The development of nanotechnology provides a new direction for the research and development of healthy human settlement materials. Nano-microcapsules are used as carriers to prepare new building coating materials with efficient antibacterial agents, which can achieve slow release and efficient antibacterial properties. It has important application value for improving bacterial pollution on indoor walls and providing a clean-living environment. Silver nanoparticles coated with nano-microcapsules (AgNPs@PS-NMP) were prepared by one-step method for killing Staphylococcus aureus ( S. aureus ). SEM results showed that the particle size was about 500–600 nm, the particle size was uniform, the degree of agglomeration was small, and the morphology was complete. The Zeta potential was approximately −18.8 mV. The coating construction performance, hydrophilicity, drying time of AgNPs@PS-NMP were investigated respectively. The antibacterial activity of AgNPs@PS-NMP was further studied. The results showed that the nano-microcapsules had a good killing effect on S. aureus , and the minimum bactericidal concentration (MBC) was 28 μ g/mL. These results suggest that AgNPs@PS-NMP, as a new type of coating, not only has good coating properties but also excellent antibacterial properties. It can be used as a potential antibacterial building material for hospitals, schools and other places.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This article aims to examine the research advancements in photodynamic therapy (PDT) for hepatocellular carcinoma (HCC) utilizing nano/microtechnology. The objective is to provide valuable insights for disease prevention and control in patients with liver cancer (LC). LC is a prevalent form of cancer that inflicts significant suffering on patients. Early treatment plays a crucial role in improving the prognosis and enhancing the quality of life (QoL) for LC patients. While PDT has proven effective in treating surface and intracerebral tumors, studies focusing on intra-abdominal tumors, particularly liver tumors, are limited. Hence, it is essential to comprehend the clinical value and therapeutic outcomes of PDT for LC, particularly in the context of nano/microtechnology. With rapid advancements in nanotechnology, its integration into the medical field has grown extensively. By leveraging nanotechnology, the analysis of PDT’s effects on HCC yields significant clinical value. This article reviews the clinical diagnostic methods and therapeutic approaches for LC, emphasizes the impact of PDT on clinical outcomes for LC patients, and analyzes the progress of PDT applied to HCC under the framework of nano/microtechnology. The findings aim to offer valuable references for the clinical treatment of LC patients.
{"title":"Progress of Photodynamic Therapy in Hepatocellular Carcinoma Using Nano/Microtechnology","authors":"Lifeng Liu, Qiang Chen","doi":"10.1166/jbn.2023.3669","DOIUrl":"https://doi.org/10.1166/jbn.2023.3669","url":null,"abstract":"This article aims to examine the research advancements in photodynamic therapy (PDT) for hepatocellular carcinoma (HCC) utilizing nano/microtechnology. The objective is to provide valuable insights for disease prevention and control in patients with liver cancer (LC). LC is a prevalent form of cancer that inflicts significant suffering on patients. Early treatment plays a crucial role in improving the prognosis and enhancing the quality of life (QoL) for LC patients. While PDT has proven effective in treating surface and intracerebral tumors, studies focusing on intra-abdominal tumors, particularly liver tumors, are limited. Hence, it is essential to comprehend the clinical value and therapeutic outcomes of PDT for LC, particularly in the context of nano/microtechnology. With rapid advancements in nanotechnology, its integration into the medical field has grown extensively. By leveraging nanotechnology, the analysis of PDT’s effects on HCC yields significant clinical value. This article reviews the clinical diagnostic methods and therapeutic approaches for LC, emphasizes the impact of PDT on clinical outcomes for LC patients, and analyzes the progress of PDT applied to HCC under the framework of nano/microtechnology. The findings aim to offer valuable references for the clinical treatment of LC patients.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"106 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Trung Hieu Le, My Uyen Dao, Thi Hong Chuong Nguyen, Thi Van Thi Tran, Lam Son Le, Thi Nhu Nguyen, Thanh Minh Tran, Hoang Luong Ngoc Nguyen, Xuan Anh Vu Ho
The presented investigation attempts to unveil the novel approach to prepare glucomannan/collagen-based hydrogel through the utilization of calcium ions (i.e., Ca 2+ ) as the cross-linker. The achieved composite provides an appropriate scaffold for the deposition of gallic acid as an active species. It turns out that gallic acid-decorated glucomannan/Ca 2+ /collagen composite (denoted as KGM/Ca 2+ /Col/GA) shows a great capacity to prevent free radicals in the antioxidant test. Impressively, the as-generated KGM/Ca 2+ /Col/GA sample demonstrates a robust capability to inhibit KB cells in the cytotoxic evaluation, associated with an extremely low IC 50 value (e.g., 8.8±0.5 μ g/mL). Such pieces of evidence suggest the potential application of KGM/Ca/Col/GA hydrogel material in medical applications.
{"title":"Calcium Ions-Driven Hydrogel Scaffold Toward the Robust Antioxidant and Anticancer Biomaterials","authors":"Trung Hieu Le, My Uyen Dao, Thi Hong Chuong Nguyen, Thi Van Thi Tran, Lam Son Le, Thi Nhu Nguyen, Thanh Minh Tran, Hoang Luong Ngoc Nguyen, Xuan Anh Vu Ho","doi":"10.1166/jbn.2023.3661","DOIUrl":"https://doi.org/10.1166/jbn.2023.3661","url":null,"abstract":"The presented investigation attempts to unveil the novel approach to prepare glucomannan/collagen-based hydrogel through the utilization of calcium ions (i.e., Ca 2+ ) as the cross-linker. The achieved composite provides an appropriate scaffold for the deposition of gallic acid as an active species. It turns out that gallic acid-decorated glucomannan/Ca 2+ /collagen composite (denoted as KGM/Ca 2+ /Col/GA) shows a great capacity to prevent free radicals in the antioxidant test. Impressively, the as-generated KGM/Ca 2+ /Col/GA sample demonstrates a robust capability to inhibit KB cells in the cytotoxic evaluation, associated with an extremely low IC 50 value (e.g., 8.8±0.5 μ g/mL). Such pieces of evidence suggest the potential application of KGM/Ca/Col/GA hydrogel material in medical applications.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiguang Xiao, Liming Xu, Bo Zheng, Zhun Wu, Jinqu Chen
We aimed to investigate the pivotal role of Tripartite Motif Containing 66 (TRIM66) in bladder cancer (BCa) and elucidate its underlying mechanism in promoting BCa cell metastasis. Tumor and adjacent normal tissues were collected from 62 BCa patients, and TRIM66 was quantified using quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between TRIM66 expression and clinical indicators, as well as patient prognosis, was analyzed. In addition, an in vitro model was established by silencing TRIM66 in a BCa cell line. The impact of TRIM66 on BCa cell invasion and metastasis was evaluated through Transwell and cell wound healing assays. Through meticulous bioinformatics analysis and luciferase assays, we confirmed that TRIM66 specifically binds to Matrix Metallopeptidase 11 (MMP11). Moreover, mRNA expression analysis revealed a positive correlation between TRIM66 and MMP11 in BCa tumor tissues. Intriguingly, in a cell recovery experiment, overexpression of MMP11 reversed the inhibition of migration and proliferation caused by TRIM66 downregulation. Collectively, our findings unequivocally indicate that heightened TRIM66 expression is closely associated with a malignant phenotype in BCa tissues. Silencing TRIM66 significantly mitigates BCa cell metastasis in vitro by downregulating MMP11. These observations shed light on the critical involvement of the TRIM66-MMP11 axis in BCa progression, offering promising avenues for therapeutic interventions targeting this pathway.
{"title":"Tripartite Motif Containing 66 Promotes Malignant Progression of Bladder Cancer by Activating Matrix Metallopeptidase 11","authors":"Jiguang Xiao, Liming Xu, Bo Zheng, Zhun Wu, Jinqu Chen","doi":"10.1166/jbn.2023.3663","DOIUrl":"https://doi.org/10.1166/jbn.2023.3663","url":null,"abstract":"We aimed to investigate the pivotal role of Tripartite Motif Containing 66 (TRIM66) in bladder cancer (BCa) and elucidate its underlying mechanism in promoting BCa cell metastasis. Tumor and adjacent normal tissues were collected from 62 BCa patients, and TRIM66 was quantified using quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between TRIM66 expression and clinical indicators, as well as patient prognosis, was analyzed. In addition, an in vitro model was established by silencing TRIM66 in a BCa cell line. The impact of TRIM66 on BCa cell invasion and metastasis was evaluated through Transwell and cell wound healing assays. Through meticulous bioinformatics analysis and luciferase assays, we confirmed that TRIM66 specifically binds to Matrix Metallopeptidase 11 (MMP11). Moreover, mRNA expression analysis revealed a positive correlation between TRIM66 and MMP11 in BCa tumor tissues. Intriguingly, in a cell recovery experiment, overexpression of MMP11 reversed the inhibition of migration and proliferation caused by TRIM66 downregulation. Collectively, our findings unequivocally indicate that heightened TRIM66 expression is closely associated with a malignant phenotype in BCa tissues. Silencing TRIM66 significantly mitigates BCa cell metastasis in vitro by downregulating MMP11. These observations shed light on the critical involvement of the TRIM66-MMP11 axis in BCa progression, offering promising avenues for therapeutic interventions targeting this pathway.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Melanoma is one of the deadliest forms of skin cancer which accounts for about 1% of all diagnosed skin cancer cases in the United States. Metastatic melanoma is highly resistant to conventional treatments and the disease is highly refractory. Therefore, the development of new treatment strategies is crucial. The current drug delivery systems for melanoma therapy have certain disadvantages such as off-target drug delivery, low circulation time, toxic side effects, and the need for repeated drug administration. To address these challenges, polymeric nanoparticles have broken new grounds for melanoma treatment. Among different candidates, chitosan-based nanoparticles (ChNPs) have gained significant attention. Due to their excellent biocompatibility, non-immunogenicity, versatile properties, high stability, and low cost, therapeutic appeal towards these carriers is on the rise. In the current review, recent progress, applications, and challenges of ChNPs in melanoma treatment will be discussed.
{"title":"Chitosan Nanoparticles as Next Generation of Drug Carriers for Melanoma Treatment: A Review","authors":"Xuesong Ren, Qi Jiang, Yali Zeng, Lin Wang","doi":"10.1166/jbn.2023.3598","DOIUrl":"https://doi.org/10.1166/jbn.2023.3598","url":null,"abstract":"Melanoma is one of the deadliest forms of skin cancer which accounts for about 1% of all diagnosed skin cancer cases in the United States. Metastatic melanoma is highly resistant to conventional treatments and the disease is highly refractory. Therefore, the development of new treatment strategies is crucial. The current drug delivery systems for melanoma therapy have certain disadvantages such as off-target drug delivery, low circulation time, toxic side effects, and the need for repeated drug administration. To address these challenges, polymeric nanoparticles have broken new grounds for melanoma treatment. Among different candidates, chitosan-based nanoparticles (ChNPs) have gained significant attention. Due to their excellent biocompatibility, non-immunogenicity, versatile properties, high stability, and low cost, therapeutic appeal towards these carriers is on the rise. In the current review, recent progress, applications, and challenges of ChNPs in melanoma treatment will be discussed.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The development of multifunctional biomaterials with precise design and synthesis is desired for various fields of medicine and biology. Nanomaterials and nanotechnology have a huge potential to be applied and develop multifunctional and sophisticated nano- and biomaterials. In this study, we synthesized silver nanoparticles (AgNPs) and applied them into Chitosan-Agarosemodified magnetic nanocomposite to develop an efficient reusable Nano catalyst. The fabricated AgNPs were characterized using Transmission electron microscopy and results showed that AgNPs were partially spherical with uniform morphology and a size of 22±6 nm. Measuring the zeta potential showed that AgNPs possess a negative surface potential of around −27.7 mV which is desired for the stability of AgNPs. The AgNPs were incorporated into a Chitosan-Agarose modified magnetic nanocomposite and TEM and EDX analysis confirmed homogenous dispersion of AgNPs into the nanocomposite. The cell culture ( in vitro ) studies conducted using the MTT assay kit confirmed the biocompatibility of the fabricated nanocomposite. The animal study (the implantation of the nanocomposite in rat calvarial defect model) showed that the nanocomposite induced bone regeneration and filed the induced defect. These resultant findings suggested that the developed AgNPs-bearing nanocomposite can be applied as an efficient reusable Nano catalyst for bone regeneration.
{"title":"Effects of Ag Nanoparticles Impregnated Over Chitosan-Agarose Modified Magnetic Nanocomposite as an Efficient Reusable Nano Catalyst on Bone Regeneration in a Rat Calvarial Defect Model and Screening System","authors":"Bingchen Liu, Hongqu Luo","doi":"10.1166/jbn.2023.3713","DOIUrl":"https://doi.org/10.1166/jbn.2023.3713","url":null,"abstract":"The development of multifunctional biomaterials with precise design and synthesis is desired for various fields of medicine and biology. Nanomaterials and nanotechnology have a huge potential to be applied and develop multifunctional and sophisticated nano- and biomaterials. In this study, we synthesized silver nanoparticles (AgNPs) and applied them into Chitosan-Agarosemodified magnetic nanocomposite to develop an efficient reusable Nano catalyst. The fabricated AgNPs were characterized using Transmission electron microscopy and results showed that AgNPs were partially spherical with uniform morphology and a size of 22±6 nm. Measuring the zeta potential showed that AgNPs possess a negative surface potential of around −27.7 mV which is desired for the stability of AgNPs. The AgNPs were incorporated into a Chitosan-Agarose modified magnetic nanocomposite and TEM and EDX analysis confirmed homogenous dispersion of AgNPs into the nanocomposite. The cell culture ( in vitro ) studies conducted using the MTT assay kit confirmed the biocompatibility of the fabricated nanocomposite. The animal study (the implantation of the nanocomposite in rat calvarial defect model) showed that the nanocomposite induced bone regeneration and filed the induced defect. These resultant findings suggested that the developed AgNPs-bearing nanocomposite can be applied as an efficient reusable Nano catalyst for bone regeneration.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135434287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to explore the potential effects of curculigoside on NLRP3 expression and catabolic genes in osteoarthritis (OA) development. OA mouse models were generated by destabilizing the medial meniscus (DMM) and treated with curculigoside. Curculigoside treatment resulted in dose-dependent reductions in OARSI scores, with the 20 μ g dose restoring scores to normal levels. Curculigoside increased mRNA and protein levels of iNOS and MMP9 induced by DMM surgery in a dose-dependent manner. Moreover, curculigoside downregulated the expression of NLRP3, NF- κ B, and PKR at both mRNA and protein levels. Additionally, curculigoside reversed the effects of IL-1 β on MMP-9, iNOS, and Col2A mRNA and protein levels in a dose-dependent manner, similar to the NLRP3 inhibitor MCC950. In vivo and in vitro results supported curculigoside’s potential to aid cartilage restoration in OA patients by blocking the NLRP3 pathway. These findings suggest curculigoside as a potential therapeutic option for OA, offering hope for improved public health outcomes related to this degenerative joint condition.
{"title":"Curculigoside Inhibits the Progression of Osteoarthritis via Regulating Nucleotide-Binding Oligomerization Domain-Like Receptor Containing Pyrin Domain 3","authors":"Guowei Shen, Shichang Yan, Siyuan Shen, Feng Liang, Shouyun Xiao, Yunpeng Zhang, Yongtao Zhang, Huimin Ding","doi":"10.1166/jbn.2023.3695","DOIUrl":"https://doi.org/10.1166/jbn.2023.3695","url":null,"abstract":"This study aimed to explore the potential effects of curculigoside on NLRP3 expression and catabolic genes in osteoarthritis (OA) development. OA mouse models were generated by destabilizing the medial meniscus (DMM) and treated with curculigoside. Curculigoside treatment resulted in dose-dependent reductions in OARSI scores, with the 20 μ g dose restoring scores to normal levels. Curculigoside increased mRNA and protein levels of iNOS and MMP9 induced by DMM surgery in a dose-dependent manner. Moreover, curculigoside downregulated the expression of NLRP3, NF- κ B, and PKR at both mRNA and protein levels. Additionally, curculigoside reversed the effects of IL-1 β on MMP-9, iNOS, and Col2A mRNA and protein levels in a dose-dependent manner, similar to the NLRP3 inhibitor MCC950. In vivo and in vitro results supported curculigoside’s potential to aid cartilage restoration in OA patients by blocking the NLRP3 pathway. These findings suggest curculigoside as a potential therapeutic option for OA, offering hope for improved public health outcomes related to this degenerative joint condition.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"220 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Breast cancer (BC) is a highly prevalent aggressive malignancy in women worldwide, and the search for key targets in its pathogenesis is a focus of research. Long non-coding RNAs (lncRNAs) play an important role in many cancers, including breast cancer. This study aimed to investigate the role of lncRNA SNHG9 in BC. The expression of SNHG9 in BC cells was found to be higher than that of human mammary epithelium. SNHG9 was found to inhibit the proliferation, migration, and invasion of cells and promote apoptosis. It was also found that SNHG9 regulates the miR-326/Wnt5a/ β -catenin axis to promote the development of BC. Dual luciferase reporter and RNA pull-down assays confirmed the interaction between SNHG9, Wnt5a, and miR-326. Western blot analysis indicated that the expression of Wnt5a, β -catenin, c-myc, and cyclin D1 decreased significantly after the silencing of SNHG9 and the overexpression of miR-326. On the contrary, Wnt5a, β -catenin, c-myc, and cyclin D1 proteins were significantly up-regulated after inhibiting miR-326 expression. These findings suggest that SNHG9 is a promising target for BC therapy.
{"title":"Small Nucleolar RNA Host Gene 9 Promotes the Development of Breast Cancer by Regulating the miR-326/Wnt5a/<i>β</i>-Catenin Axis","authors":"Jian Wang, Hekai Chen, Zhihua Jia","doi":"10.1166/jbn.2023.3665","DOIUrl":"https://doi.org/10.1166/jbn.2023.3665","url":null,"abstract":"Breast cancer (BC) is a highly prevalent aggressive malignancy in women worldwide, and the search for key targets in its pathogenesis is a focus of research. Long non-coding RNAs (lncRNAs) play an important role in many cancers, including breast cancer. This study aimed to investigate the role of lncRNA SNHG9 in BC. The expression of SNHG9 in BC cells was found to be higher than that of human mammary epithelium. SNHG9 was found to inhibit the proliferation, migration, and invasion of cells and promote apoptosis. It was also found that SNHG9 regulates the miR-326/Wnt5a/ β -catenin axis to promote the development of BC. Dual luciferase reporter and RNA pull-down assays confirmed the interaction between SNHG9, Wnt5a, and miR-326. Western blot analysis indicated that the expression of Wnt5a, β -catenin, c-myc, and cyclin D1 decreased significantly after the silencing of SNHG9 and the overexpression of miR-326. On the contrary, Wnt5a, β -catenin, c-myc, and cyclin D1 proteins were significantly up-regulated after inhibiting miR-326 expression. These findings suggest that SNHG9 is a promising target for BC therapy.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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