Gastric cancer (GC) is one of the most frequent cancers, resulting in plenty of death worldwide. Brassinin is a plant antibiotic extracted from cruciferous vegetables and exerts a vital anticancer role in various cancers. Nevertheless, its function and mechanism in GC are still uncovered. The role of brassinin in proliferation in GC cells were tested with cell counting kit 8 (CCK-8) or 5-ethynyl-2′-deoxyuridine (EdU) assay. The apoptotic cells were observed utilizing flow cytometry assay. Transwell analysis was introduced to assess GC cell migration as well as invasion. The downstream pathway regulated via brassinin in GC cells was determined utilizing Cignal Finder RTK signaling 10-Pathway Reporter Array and verified with Western blot assay. Brassinin reduced GC cell viability in a concentrated-dependent way. The brassinin treatment blocked cell migration in addition to invasion, and induced apoptosis in GC cells. Additionally, brassinin administration inactivated the NF- κ B cascade signal. Meanwhile, NF- κ B activator counteracted the effects of brassinin on GC cell progression. This investigation pointed out that brassinin inhibited growth and induced apoptosis at least partly by mediating NF- κ B pathway in GC cells, which indicated that brassinin will be a promising drug for treating GC.
{"title":"Brassinin Inhibits Progression and Induces Apoptosis in Gastric Cancer Cells by Regulating NF-<i>κ</i>B Signaling Cascade","authors":"Jianwen Li","doi":"10.1166/jbn.2023.3629","DOIUrl":"https://doi.org/10.1166/jbn.2023.3629","url":null,"abstract":"Gastric cancer (GC) is one of the most frequent cancers, resulting in plenty of death worldwide. Brassinin is a plant antibiotic extracted from cruciferous vegetables and exerts a vital anticancer role in various cancers. Nevertheless, its function and mechanism in GC are still uncovered. The role of brassinin in proliferation in GC cells were tested with cell counting kit 8 (CCK-8) or 5-ethynyl-2′-deoxyuridine (EdU) assay. The apoptotic cells were observed utilizing flow cytometry assay. Transwell analysis was introduced to assess GC cell migration as well as invasion. The downstream pathway regulated via brassinin in GC cells was determined utilizing Cignal Finder RTK signaling 10-Pathway Reporter Array and verified with Western blot assay. Brassinin reduced GC cell viability in a concentrated-dependent way. The brassinin treatment blocked cell migration in addition to invasion, and induced apoptosis in GC cells. Additionally, brassinin administration inactivated the NF- κ B cascade signal. Meanwhile, NF- κ B activator counteracted the effects of brassinin on GC cell progression. This investigation pointed out that brassinin inhibited growth and induced apoptosis at least partly by mediating NF- κ B pathway in GC cells, which indicated that brassinin will be a promising drug for treating GC.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The nanofibre membrane with the antibacterial doxycycline (DOX) intermediate layer can direct tissue regeneration and have antibacterial properties. Through the use of electrospinning, we were able to create DOX-loaded guided tissue regeneration (GTR)/guided bone regeneration (GBR) membranes and assess their efficacy in the treatment of peri-implantitis. Methods: Electrostatic spinning was used to create DOX-containing poly(caprolactone) (PCL) nanofibres, and a scanning electron microscope (SEM) was used to examine the membranes’ surface morphology. Results: The synthesised DOX-PCL nanofibres were found to have a smooth surface and a uniform diameter distribution, as revealed by scanning electron microscopy. When the percentage of DOX was raised from 15% to 25%, the fibre diameter shrank from 247.17 nm to 181.34 nm. According to differential calorimetry, the heat absorption peaks for the pre-electrospun PCL and 10% PCL electrospun membranes were most pronounced at 66 °C, while the peaks for the DOX powder showed at 171.5 °C. With the shift in drug loading, carbonization occurred at 223.5 degrees Celsius. PCL underwent a thermal reaction between 60 and 66 degrees Celsius. However, medication loading was strongly correlated with the level of suppression. Agar diffusion results showed that DOX-loaded nanofibre membranes inhibited the growth of Actinomyces (Aa) and Porphyromonas gingivalis; for both bacterial species, the diameter of the inhibition zone grew larger when more drug was added to the membranes. After 1 hour, 4 hours, 12 hours, 1 day, 7 days, and 28 days of the experiment, the cumulative drug release rates of CL+DOX were 19.14%, 36.16%, 44.37%, 59.52%, and 65.150%, respectively. After the initial steep drop during the first three days of the trial, the PCL-DOX release rate stabilised at around 1.61 percent per hour. Minimum concentration was 3.13 g/mL, while PCL-DOX release rate fell from 1.28 percent on Day 4 to 0.51 percent on Day 28. Conclusion: Combining the availability of natural polymeric materials with the reliability of manufactured polymeric materials, the three-layer nanofibre membranes use PCL-GE as a double surface layer and PCL-DOX as an intermediate layer. In addition, the physical insulating qualities of these membranes are maintained, which allows the fibre membrane to perform a sustained antibacterial action and prevents the initial, abrupt release of the medication in the tissue. Peri-implantitis may be treatable with the use of both pharmaceuticals and GTR/GBR technology.
{"title":"Preparation of Doxycycline Hydrochloride Guided Tissue Regeneration/Guided Bone Regeneration Membranes Through Electrostatic Spinning for the Treatment of Peri-Implantitis","authors":"Geng Liu, Jianbo Li, Xue Shi","doi":"10.1166/jbn.2023.3618","DOIUrl":"https://doi.org/10.1166/jbn.2023.3618","url":null,"abstract":"Objective: The nanofibre membrane with the antibacterial doxycycline (DOX) intermediate layer can direct tissue regeneration and have antibacterial properties. Through the use of electrospinning, we were able to create DOX-loaded guided tissue regeneration (GTR)/guided bone regeneration (GBR) membranes and assess their efficacy in the treatment of peri-implantitis. Methods: Electrostatic spinning was used to create DOX-containing poly(caprolactone) (PCL) nanofibres, and a scanning electron microscope (SEM) was used to examine the membranes’ surface morphology. Results: The synthesised DOX-PCL nanofibres were found to have a smooth surface and a uniform diameter distribution, as revealed by scanning electron microscopy. When the percentage of DOX was raised from 15% to 25%, the fibre diameter shrank from 247.17 nm to 181.34 nm. According to differential calorimetry, the heat absorption peaks for the pre-electrospun PCL and 10% PCL electrospun membranes were most pronounced at 66 °C, while the peaks for the DOX powder showed at 171.5 °C. With the shift in drug loading, carbonization occurred at 223.5 degrees Celsius. PCL underwent a thermal reaction between 60 and 66 degrees Celsius. However, medication loading was strongly correlated with the level of suppression. Agar diffusion results showed that DOX-loaded nanofibre membranes inhibited the growth of Actinomyces (Aa) and Porphyromonas gingivalis; for both bacterial species, the diameter of the inhibition zone grew larger when more drug was added to the membranes. After 1 hour, 4 hours, 12 hours, 1 day, 7 days, and 28 days of the experiment, the cumulative drug release rates of CL+DOX were 19.14%, 36.16%, 44.37%, 59.52%, and 65.150%, respectively. After the initial steep drop during the first three days of the trial, the PCL-DOX release rate stabilised at around 1.61 percent per hour. Minimum concentration was 3.13 g/mL, while PCL-DOX release rate fell from 1.28 percent on Day 4 to 0.51 percent on Day 28. Conclusion: Combining the availability of natural polymeric materials with the reliability of manufactured polymeric materials, the three-layer nanofibre membranes use PCL-GE as a double surface layer and PCL-DOX as an intermediate layer. In addition, the physical insulating qualities of these membranes are maintained, which allows the fibre membrane to perform a sustained antibacterial action and prevents the initial, abrupt release of the medication in the tissue. Peri-implantitis may be treatable with the use of both pharmaceuticals and GTR/GBR technology.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jie Yang, Lu Yao, Sai He, Periyannan Velu, Annamalai Vijayalakshmi, Run-Xu Yang, Hong-xuan Ren
Zinc oxide nanoparticles (ZnO-NPs) were synthesized in this study using Solanum xanthocarpum ( Sx ) leaf extracts via a green method. The nanomaterial, ZnO-NPs- Sx , was prepared using zinc acetate dehydrate solution followed by purification. The nanofabricate size and structural properties were investigated using UV-V Spectroscopy, X-ray diffraction, energy dispersive X-ray, and Fourier transform infrared spectroscopy (FT-IR), as well as scanning electron microscopy (SEM). The anti-neoplastic properties of nanomaterial against the colon cancer cells (HT-29) were measured using MTT assay and the apoptotic biomarkers via staining techniques (i.e., acridine orange/ethidium bromide (AO/EB), 4′,6-diamidino-2-phenylindole (DAPI) and Propidium iodide (PI staining). The nanomaterial induced reactive oxygen species ROS and apoptosis by stimulating cell signaling biomarkers: caspase-3, -8 and -9. Staining showed proportionately higher number of dead cells with nuclear damage and chromatin condensation to confirm the nanomaterial induced apoptosis in HT-29 cells. At low concentrations, the nanomaterial did not elicit adverse cellular changes, and was less toxic towards HT-29 cells. Thus, the bio-synthesized nanofabricate, can be used ZnO-NPs- Sx in nanomedicine to improve current treatments towards cancer cells.
{"title":"Zinc Oxide Nanoparticles Produced Sustainably Utilizing <i>Solanum xanthocarpum</i> Leaf Extracts and Their Anti-Neoplastic Effects on Colon Cancer Cells (HT-29)","authors":"Jie Yang, Lu Yao, Sai He, Periyannan Velu, Annamalai Vijayalakshmi, Run-Xu Yang, Hong-xuan Ren","doi":"10.1166/jbn.2023.3634","DOIUrl":"https://doi.org/10.1166/jbn.2023.3634","url":null,"abstract":"Zinc oxide nanoparticles (ZnO-NPs) were synthesized in this study using Solanum xanthocarpum ( Sx ) leaf extracts via a green method. The nanomaterial, ZnO-NPs- Sx , was prepared using zinc acetate dehydrate solution followed by purification. The nanofabricate size and structural properties were investigated using UV-V Spectroscopy, X-ray diffraction, energy dispersive X-ray, and Fourier transform infrared spectroscopy (FT-IR), as well as scanning electron microscopy (SEM). The anti-neoplastic properties of nanomaterial against the colon cancer cells (HT-29) were measured using MTT assay and the apoptotic biomarkers via staining techniques (i.e., acridine orange/ethidium bromide (AO/EB), 4′,6-diamidino-2-phenylindole (DAPI) and Propidium iodide (PI staining). The nanomaterial induced reactive oxygen species ROS and apoptosis by stimulating cell signaling biomarkers: caspase-3, -8 and -9. Staining showed proportionately higher number of dead cells with nuclear damage and chromatin condensation to confirm the nanomaterial induced apoptosis in HT-29 cells. At low concentrations, the nanomaterial did not elicit adverse cellular changes, and was less toxic towards HT-29 cells. Thus, the bio-synthesized nanofabricate, can be used ZnO-NPs- Sx in nanomedicine to improve current treatments towards cancer cells.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The death rate from gastric cancer (GC) is increasing while the methods of early diagnosis and treatment of GC are still limited. CircRNAs have ability to bind with miRNA to exert therapeutic action on kinds of cancers. The purpose of this study was to explore the action mechanism of circ-CCDC66 in GC. CCDC66, miR-129-5p and LHX2 mRNA and protein expression were examine by qRT-PCR and Western blot. Flow cytometry and Western blot were used to identify cells apoptosis. Dual-luciferase reporter assay was applied to verified the binding site that miR-129-5p and CCDC66 or LHX2. Transwell assay and cell account kit 8 (CCK-8) were used to examined cells proliferation ability, migration or invasion ability. Compared with normal tissues, CCDC66 expression was obviously higher and miR-129-5p expression was significantly lower in GC tissues. Knockdown circ-CCDC66 changed malignant behavior of GC cells. MiR-129-5p inhibitor changed the effect of down-regulated circ-CCDC66 on malignant behavior of gastric cancer cells. LHX2 was bond with miR-129-5p, and circ-CCDC66 regulated LHX2 expression to participated in GC progression via miR-129-5p. All the findings suggested that CCDC66 could adjust LHX2 expression to promote GC progression through restraining miR-129-5p, which may provide a key strategy for GC therapy.
{"title":"CircRNA Coiled-Coil Domain Containing 66 Up-Regulates LIM-Homeodomain Gene 2 to Promote Gastric Cancer Progression via Sponing miR-129-5p","authors":"Mingzhi Cai, Qiuxian Chen, Lisheng Cai, Yuqin Sun, Wenshan Zhang","doi":"10.1166/jbn.2023.3662","DOIUrl":"https://doi.org/10.1166/jbn.2023.3662","url":null,"abstract":"The death rate from gastric cancer (GC) is increasing while the methods of early diagnosis and treatment of GC are still limited. CircRNAs have ability to bind with miRNA to exert therapeutic action on kinds of cancers. The purpose of this study was to explore the action mechanism of circ-CCDC66 in GC. CCDC66, miR-129-5p and LHX2 mRNA and protein expression were examine by qRT-PCR and Western blot. Flow cytometry and Western blot were used to identify cells apoptosis. Dual-luciferase reporter assay was applied to verified the binding site that miR-129-5p and CCDC66 or LHX2. Transwell assay and cell account kit 8 (CCK-8) were used to examined cells proliferation ability, migration or invasion ability. Compared with normal tissues, CCDC66 expression was obviously higher and miR-129-5p expression was significantly lower in GC tissues. Knockdown circ-CCDC66 changed malignant behavior of GC cells. MiR-129-5p inhibitor changed the effect of down-regulated circ-CCDC66 on malignant behavior of gastric cancer cells. LHX2 was bond with miR-129-5p, and circ-CCDC66 regulated LHX2 expression to participated in GC progression via miR-129-5p. All the findings suggested that CCDC66 could adjust LHX2 expression to promote GC progression through restraining miR-129-5p, which may provide a key strategy for GC therapy.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, we explored the curative effect of recombinant human interferon α 2b gel combined with a loop electrosurgical excision procedure (LEEP) knife to treat cervicitis. We also analyzed the clinical applications of a copper/low-density polyethylene nanocomposite intrauterine device (IUD). We selected 62 patients with IUD-induced cervicitis admitted to our hospital from March 2020 to December 2022 and divided them into experimental and control groups according to the random number table method ( n = 31/group). The control group was treated with recombinant human interferon α 2b gel only, while the experimental group was treated using a combination of the gel and LEEP knife. We compared the total efficacy rate of both treatments by recording the serum inflammatory cytokines levels, hospitalization, and vaginal drainage times before and after three months of treatment. We selected 50 married women of childbearing age who came to our hospital voluntarily to receive IUDs during the same period and randomly divided them into two groups ( n = 25/group). Each group was administered either a copper/low-density polyethylene (Cu/LDPE) nanocomposite intrauterine device (IUD) or a TCu220C IUD. We conducted follow-ups at three, six, and 12 months after the implantation and analyzed the clinical and side effects. We observed that the total efficacy rate of the cervicitis treatment in the experimental group was 93%, 72% higher than in the control group. After six months of treatment, the tumor necrosis factor- α (TNF- α ), hypersensitive C-reactive protein (hs-CRP), and interleukin-6 (IL-6) levels, and the leukocyte counts of both groups were lower than that before treatment, with the test group showing lower values than the control group. Simultaneously, the hospitalization and vaginal discharge times of the experimental group were lower than those of the control group. We also followed up on the two different IUD groups after three, six, and 12 months. Compared with TCu220C IUD, the Cu/LDPE nanocomposite IUD group showed lesser side effects, such as uterine bleeding and abdominal pain. There was no significant difference between the hemoglobin levels of both groups before and after IUD insertion. In conclusion, recombinant human interferon α 2b gel combined with a LEEP knife is effective in treating cervicitis patients and can control the levels of inflammatory factors in the body. This treatment was safer and quicker and shortened the vaginal discharge time. Additionally, the clinical acceptance of Cu/LDPE nanocomposite IUD for human intrauterine contraception is good due to the low occurrence of adverse reactions, such as uterine bleeding and pain. Therefore, it should be promoted in clinical gynecological practice.
{"title":"Observation of the Curative Effect of Recombinant Human Interferon <i>α</i>2b Gel Combined with LEEP Knife in Cervicitis Caused by IUD and The Effect of Copper/Low-Density Polyethylene Nanocomposite IUD","authors":"Wei Huang, Shanshan Lu, Ling Chen, Ying Lin","doi":"10.1166/jbn.2023.3648","DOIUrl":"https://doi.org/10.1166/jbn.2023.3648","url":null,"abstract":"In this study, we explored the curative effect of recombinant human interferon α 2b gel combined with a loop electrosurgical excision procedure (LEEP) knife to treat cervicitis. We also analyzed the clinical applications of a copper/low-density polyethylene nanocomposite intrauterine device (IUD). We selected 62 patients with IUD-induced cervicitis admitted to our hospital from March 2020 to December 2022 and divided them into experimental and control groups according to the random number table method ( n = 31/group). The control group was treated with recombinant human interferon α 2b gel only, while the experimental group was treated using a combination of the gel and LEEP knife. We compared the total efficacy rate of both treatments by recording the serum inflammatory cytokines levels, hospitalization, and vaginal drainage times before and after three months of treatment. We selected 50 married women of childbearing age who came to our hospital voluntarily to receive IUDs during the same period and randomly divided them into two groups ( n = 25/group). Each group was administered either a copper/low-density polyethylene (Cu/LDPE) nanocomposite intrauterine device (IUD) or a TCu220C IUD. We conducted follow-ups at three, six, and 12 months after the implantation and analyzed the clinical and side effects. We observed that the total efficacy rate of the cervicitis treatment in the experimental group was 93%, 72% higher than in the control group. After six months of treatment, the tumor necrosis factor- α (TNF- α ), hypersensitive C-reactive protein (hs-CRP), and interleukin-6 (IL-6) levels, and the leukocyte counts of both groups were lower than that before treatment, with the test group showing lower values than the control group. Simultaneously, the hospitalization and vaginal discharge times of the experimental group were lower than those of the control group. We also followed up on the two different IUD groups after three, six, and 12 months. Compared with TCu220C IUD, the Cu/LDPE nanocomposite IUD group showed lesser side effects, such as uterine bleeding and abdominal pain. There was no significant difference between the hemoglobin levels of both groups before and after IUD insertion. In conclusion, recombinant human interferon α 2b gel combined with a LEEP knife is effective in treating cervicitis patients and can control the levels of inflammatory factors in the body. This treatment was safer and quicker and shortened the vaginal discharge time. Additionally, the clinical acceptance of Cu/LDPE nanocomposite IUD for human intrauterine contraception is good due to the low occurrence of adverse reactions, such as uterine bleeding and pain. Therefore, it should be promoted in clinical gynecological practice.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135433795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haishan Yao, Xin Guo, Qun Yao, Jiongjiong Lu, Zhenxing Sun
The objective of this study is to investigate the adjuvant effect of UCNP-PEG-M540 based on a core–shell structure in spinal cord injury (SCI) treatment. 90 female healthy adult SD rats were divided into a control (Ctrl) group, a UCNP, and a UCNP-PEG-M540 group according to different transplantation materials to observe the recovery of behavioral function and axonal regeneration in rats to evaluate the application effect. The diameter of the UCNP was approximately 24 nm, and the distribution was uniform, which conformed to nanoscale particles. The diffraction pattern of UCNP based on the core–shell structure was basically the same as that of the UCNP naked core. At 4–12 weeks of treatment, the BBB score of the UCNP-PEG-M540 group was higher based on that of the Ctrl and UCNP groups; the IOD value of NF200-positive staining in the UCNP-PEG-M540 group was much higher to that in the Ctrl group and UCNP group; the IOD value of GFAP-positive staining and the gray value of CFAP were lower to those of the Ctrl group and UCNP group. UCNP-PEG-M540 based on a core–shell structure can further promote the absorption of 980 nm near-infrared light in injured tissues of SCI rats and inhibit glial cell aggregation, thereby reducing the formation of glial cells and promoting the repair of SCI and the recovery of behavioral function.
{"title":"Preparation of Upconversion Nanoparticles (UCNPs)-Polyethylene Glycol (PEG)/Merocyanine540 (M540) Photosensitizer by Core–Shell Structure and Its Application in the Treatment of Spinal Cord Injury","authors":"Haishan Yao, Xin Guo, Qun Yao, Jiongjiong Lu, Zhenxing Sun","doi":"10.1166/jbn.2023.3671","DOIUrl":"https://doi.org/10.1166/jbn.2023.3671","url":null,"abstract":"The objective of this study is to investigate the adjuvant effect of UCNP-PEG-M540 based on a core–shell structure in spinal cord injury (SCI) treatment. 90 female healthy adult SD rats were divided into a control (Ctrl) group, a UCNP, and a UCNP-PEG-M540 group according to different\u0000 transplantation materials to observe the recovery of behavioral function and axonal regeneration in rats to evaluate the application effect. The diameter of the UCNP was approximately 24 nm, and the distribution was uniform, which conformed to nanoscale particles. The diffraction pattern of\u0000 UCNP based on the core–shell structure was basically the same as that of the UCNP naked core. At 4–12 weeks of treatment, the BBB score of the UCNP-PEG-M540 group was higher based on that of the Ctrl and UCNP groups; the IOD value of NF200-positive staining in the UCNP-PEG-M540\u0000 group was much higher to that in the Ctrl group and UCNP group; the IOD value of GFAP-positive staining and the gray value of CFAP were lower to those of the Ctrl group and UCNP group. UCNP-PEG-M540 based on a core–shell structure can further promote the absorption of 980 nm near-infrared\u0000 light in injured tissues of SCI rats and inhibit glial cell aggregation, thereby reducing the formation of glial cells and promoting the repair of SCI and the recovery of behavioral function.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48163235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To effectively treat severe diabetic foot (DF) and reduce amputation risk, we explored nano-silver alginate (Ag/Alg) dressings and tibial transverse transport (TTT) to improve circulation and suppress inflammatory responses in lower limbs. First, Ag/Alg dressings were biocompatible without affecting L-929 cell activity, and they had better hygroscopic properties when compared with conventional dressings. Ag/Alg dressing also markedly inhibited Gram+/Gram− bacterial activity. We also retrospectively analyzed clinical data from DF patients at our hospital and divided them into control and TTT groups according to treatment modality; the latter group was treated with TTT and Ag/Alg dressings as conventional therapy. We then compared cytokines, inflammatory responses, blood supply to the foot, and ulcer healing characteristics between pre- and post-treatments. TTT patients displayed significantly higher angiogenesis-related cytokine levels and more effective inflammation control after treatment when compared with patients receiving conventional therapy. Additionally, more significant increases in ankle-brachial index and skin temperature, and a greater decrease in the visual analog scale were observed in TTT patients. In terms of surface ulcer healing, TTT patients showed better healing, with healing times, amputation rates, and ulcer recurrence rates significantly reduced. Therefore, Ag/Alg dressings combined with TTT showed great potential for severe DF therapy.
{"title":"The Efficacy of Nano-Silver Alginate Dressing Combined with Tibial Transverse Transport for Stage III–IV Diabetic Foot Treatment","authors":"Yanqing Liu, Jianguo Wang, Youcai Liu, Meizhen Wu, Yuelong Wu, Yijun Zhang","doi":"10.1166/jbn.2023.3659","DOIUrl":"https://doi.org/10.1166/jbn.2023.3659","url":null,"abstract":"To effectively treat severe diabetic foot (DF) and reduce amputation risk, we explored nano-silver alginate (Ag/Alg) dressings and tibial transverse transport (TTT) to improve circulation and suppress inflammatory responses in lower limbs. First, Ag/Alg dressings were biocompatible\u0000 without affecting L-929 cell activity, and they had better hygroscopic properties when compared with conventional dressings. Ag/Alg dressing also markedly inhibited Gram+/Gram− bacterial activity. We also retrospectively analyzed clinical data from DF patients at our hospital and divided\u0000 them into control and TTT groups according to treatment modality; the latter group was treated with TTT and Ag/Alg dressings as conventional therapy. We then compared cytokines, inflammatory responses, blood supply to the foot, and ulcer healing characteristics between pre- and post-treatments.\u0000 TTT patients displayed significantly higher angiogenesis-related cytokine levels and more effective inflammation control after treatment when compared with patients receiving conventional therapy. Additionally, more significant increases in ankle-brachial index and skin temperature, and a\u0000 greater decrease in the visual analog scale were observed in TTT patients. In terms of surface ulcer healing, TTT patients showed better healing, with healing times, amputation rates, and ulcer recurrence rates significantly reduced. Therefore, Ag/Alg dressings combined with TTT showed great\u0000 potential for severe DF therapy.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46657806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Noroviruses (NoVs) is the main cause of gastroenteritis in humans worldwide, mainly affecting school-age children and adults. NoVs are transmitted through feces and vomitus, including human contact, food, and water. Presently, NoVs are detected using molecular biological methods. Loop-mediated isothermal amplification (LAMP), specifically, requires little detection equipment, a short detection time, and low technical skills. Here, we established our own NoV reverse transcription (RT) polymerase chain reaction (PCR) quantitative detection system and a NoV GII RT-LAMP detection system. We collected 40 clinical samples, extracted RNAs, and used RT-PCR and RT-LAMP to detect NoV GII. The qualitative results of RT-LAMP were consistent with those of RT-PCR. However, a significant difference was observed between RT-LAMP and RT-PCR quantitative detection results. The NoV GII RT-LAMP detection system showed good sensitivity, up to 101, as well as good specificity. Furthermore, GI and GII did not interfere with each other. No false-positive responses were obtained for other gastrointestinal RNA viruses, such as Coxsackie virus A16 or enterovirus 71. Our results showed that the RT-LAMP detection system for NoV GII is suitable for the quantitative determination of NoV.
{"title":"Rapid Detection of Norovirus GII by Fluorescent Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) and Nanomagnetic Bead Separation","authors":"Zhengkang Li, Yu-Wei Di, Xiaoyan Song, Yanqi Wu, Yingye Feng, Xinqiang Zhang, Cai-ting Gong, Guanghua Li","doi":"10.1166/jbn.2023.3552","DOIUrl":"https://doi.org/10.1166/jbn.2023.3552","url":null,"abstract":"Noroviruses (NoVs) is the main cause of gastroenteritis in humans worldwide, mainly affecting school-age children and adults. NoVs are transmitted through feces and vomitus, including human contact, food, and water. Presently, NoVs are detected using molecular biological methods. Loop-mediated\u0000 isothermal amplification (LAMP), specifically, requires little detection equipment, a short detection time, and low technical skills. Here, we established our own NoV reverse transcription (RT) polymerase chain reaction (PCR) quantitative detection system and a NoV GII RT-LAMP detection system.\u0000 We collected 40 clinical samples, extracted RNAs, and used RT-PCR and RT-LAMP to detect NoV GII. The qualitative results of RT-LAMP were consistent with those of RT-PCR. However, a significant difference was observed between RT-LAMP and RT-PCR quantitative detection results. The NoV GII RT-LAMP\u0000 detection system showed good sensitivity, up to 101, as well as good specificity. Furthermore, GI and GII did not interfere with each other. No false-positive responses were obtained for other gastrointestinal RNA viruses, such as Coxsackie virus A16 or enterovirus 71. Our results\u0000 showed that the RT-LAMP detection system for NoV GII is suitable for the quantitative determination of NoV.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48761926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Reyhan Koyuncu, G. Duruksu, B. Ozcelik, Serap Mert, Y. Yazır
Complexes generated by oleic acid and milk α-lactalbumin, termed BAMLET, attracted attention because of their selective toxicity against a variety of tumors. However, the production efficiency of BAMLET needs to be increased. In this study, α-lactalbumin and hydrolyzed α-lactalbumin were separately combined with oleic acid to obtain BAMLET and hydrolyzed BAMLET (HBAMLET) respectively. For these complexes, nanoparticles were prepared using double emulsion method and PLGA polymer as carrier. Then the tumoricidal activity and toxicity of the BAMLET and HBAMLET complexes were analyzed on prostate cancer cells (DU145) and breast cancer cells (MCF7) In-Vitro. The most effective concentration of HBAMLET was found as 6.38 μg/mL, at which the viability of cancer cell lines was reduced to 64.63% (for MCF7) and 47.7% (for DU145). However, BAMLET was found to be less effective, reducing DU145 and MCF7 cell viability by 9.6% and 39.5% of at 2.14 μg/mL and 10 μg/mL, respectively. Unfortunately, BAMLET showed cytotoxicity on fibroblasts at higher concentrations. Encapsulated BAMLET and HBAMLET showed promising encapsulation efficiency (72.75% and 84.44%, respectively) with a low PDI value (0.098–0.096, respectively). It was concluded that the release of HBAMLET can be controlled and can be used as an active drug agent when it was loaded in PLGA-NPs.
{"title":"Effect of Peptide-Lipid Conjugates Loaded PLGA Nanoparticles Against Cancer Cells In-Vitro","authors":"Reyhan Koyuncu, G. Duruksu, B. Ozcelik, Serap Mert, Y. Yazır","doi":"10.1166/jbn.2023.3588","DOIUrl":"https://doi.org/10.1166/jbn.2023.3588","url":null,"abstract":"Complexes generated by oleic acid and milk α-lactalbumin, termed BAMLET, attracted attention because of their selective toxicity against a variety of tumors. However, the production efficiency of BAMLET needs to be increased. In this study, α-lactalbumin and\u0000 hydrolyzed α-lactalbumin were separately combined with oleic acid to obtain BAMLET and hydrolyzed BAMLET (HBAMLET) respectively. For these complexes, nanoparticles were prepared using double emulsion method and PLGA polymer as carrier. Then the tumoricidal activity and toxicity\u0000 of the BAMLET and HBAMLET complexes were analyzed on prostate cancer cells (DU145) and breast cancer cells (MCF7) In-Vitro. The most effective concentration of HBAMLET was found as 6.38 μg/mL, at which the viability of cancer cell lines was reduced to 64.63% (for MCF7) and\u0000 47.7% (for DU145). However, BAMLET was found to be less effective, reducing DU145 and MCF7 cell viability by 9.6% and 39.5% of at 2.14 μg/mL and 10 μg/mL, respectively. Unfortunately, BAMLET showed cytotoxicity on fibroblasts at higher concentrations. Encapsulated BAMLET\u0000 and HBAMLET showed promising encapsulation efficiency (72.75% and 84.44%, respectively) with a low PDI value (0.098–0.096, respectively). It was concluded that the release of HBAMLET can be controlled and can be used as an active drug agent when it was loaded in PLGA-NPs.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44164759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the current study, Morinda citrifolia extract was loaded into gelatin-based electrospun scaffolds to treat diabetic wounds. The produced scaffolds were characterized in vitro using electron microscopy, cell culture studies, antimicrobial assessment, tensile strength assay, and water uptake capacity measurements. Wound healing function of these dressings was evaluated in a rat model of diabetic wound. Study showed that Morinda citrifolia extract-loaded wound dressings promoted diabetic wound healing by increasing wound size reduction rate and collagen deposition.
{"title":"Electrospun Morinda citrifolia Extract-Loaded Wound Dressings Promote Diabetic Wound Healing Through Antiinflammatory and Antioxidative Activities","authors":"Wenhua Ma, Zhongzhuang Pang, Yonglin Zhao","doi":"10.1166/jbn.2023.3632","DOIUrl":"https://doi.org/10.1166/jbn.2023.3632","url":null,"abstract":"In the current study, Morinda citrifolia extract was loaded into gelatin-based electrospun scaffolds to treat diabetic wounds. The produced scaffolds were characterized in vitro using electron microscopy, cell culture studies, antimicrobial assessment, tensile strength\u0000 assay, and water uptake capacity measurements. Wound healing function of these dressings was evaluated in a rat model of diabetic wound. Study showed that Morinda citrifolia extract-loaded wound dressings promoted diabetic wound healing by increasing wound size reduction rate and collagen\u0000 deposition.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48236698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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