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Genome wide association mapping of end-use gluten properties in bread wheat landraces (Triticum aestivum L.) 面包小麦陆地品系(Triticum aestivum L.)最终用途面筋特性的全基因组关联图谱
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-06-11 DOI: 10.1016/j.jcs.2024.103956
Matilde López-Fernández , Alejandro Chozas , Elena Benavente , Elia Alonso-Rueda , Julio Isidro y Sánchez , Laura Pascual , Patricia Giraldo

Numerous studies have aimed to dissect the genetic basis of wheat end-use quality, yet the consideration of gluten aggregation properties in this context has been limited despite its important role in determining the processing quality. This study has addressed the evaluation of the end-use quality of a collection of 189 Spanish bread wheat (Triticum aestivum L.) landraces. For phenotyping, we utilized the GlutoPeak methodology to assess gluten aggregation traits, the SDS-sedimentation test to measure gluten strength and NIR for grain protein content. In addition to previous genotyping data with numerous DArT-seq markers distributed across the entire wheat genome, we developed ten KASP-based molecular markers designed for GLU-1 loci, which encode high molecular weight glutenins (HMW-GS). The reliability of these markers was thoroughly assessed. Our genome-wide association analysis (GWAS) focused on gluten properties and protein content, revealed 89 marker-trait-associated loci (MTAs). These loci were further grouped into 50 MTA-QTLs across various chromosomes based on linkage disequilibrium. While some MTA-QTLs overlapped with regions identified in other studies, others represented novel genomic regions. Inside these regions, we identified several candidate genes with gluten quality-related annotated functions or grain-specific expression patterns, which represent potential targets for marker development and future wheat end-use quality improvement.

许多研究旨在剖析小麦最终使用质量的遗传基础,但在这方面对面筋聚集特性的考虑却很有限,尽管它在决定加工质量方面起着重要作用。本研究评估了 189 个西班牙面包小麦(Triticum aestivum L.)陆生品系的最终使用质量。在表型分析方面,我们采用 GlutoPeak 方法评估面筋聚集性状,采用 SDS 沉淀测试测量面筋强度,并采用近红外光谱测量谷物蛋白质含量。除了之前用分布在整个小麦基因组的大量 DArT-seq 标记进行基因分型的数据外,我们还开发了 10 个基于 KASP 的分子标记,这些标记是为编码高分子量谷蛋白(HMW-GS)的 GLU-1 位点设计的。我们对这些标记的可靠性进行了全面评估。我们的全基因组关联分析(GWAS)侧重于谷蛋白特性和蛋白质含量,发现了 89 个标记性状相关位点(MTAs)。根据连锁不平衡,这些位点在不同的染色体上被进一步分组为 50 个 MTA-QTL。有些 MTA-QTL 与其他研究发现的区域重叠,有些则代表了新的基因组区域。在这些区域中,我们发现了几个候选基因,它们具有与谷蛋白品质相关的注释功能或谷粒特异性表达模式,是标记开发和未来小麦最终用途品质改良的潜在目标。
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引用次数: 0
Enhancing the quality of highland barley noodles by Lactobacillus plantarum pre-fermentation: Insights into its underlying mechanism 通过植物乳杆菌预发酵提高高原大麦面条的质量:对其潜在机制的见解
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-06-11 DOI: 10.1016/j.jcs.2024.103954
Yu-Heng Wang , Yi-Peng Bai , Xiao-Na Guo , Ke-Xue Zhu

To improve highland barley noodles (HBNs) quality, the highland barley flour (HBF) was pre-fermented by Lactobacillus plantarum in this study. The changes in the quality of HBNs, the hydration properties and microstructure of the dietary fiber (DF), and the conformation and cross-linking of the protein in HBNs were measured. With the extension of pre-fermentation time (0–4 h), the overall acceptance of HBNs increased from 7.83 to 8.27, along with a 5.9% reduction in hardness and a 4.5% increase in springiness of HBNs. Meanwhile, confocal laser scanning microscopy (CLSM) revealed that the physical structure of the DF gradually disintegrated, which caused a significant (p < 0.05) decrease in its water retention capacity. In addition, spectral analysis showed a continual increase (from 19.62% to 30.15%) in β-turn content of the protein. The content of the free sulfhydryl group and sodium dodecyl sulfate extractable protein (SDSEP) decreased by 18.1% and 4.12%, respectively. However, with the further extension of pre-fermentation time (6–8 h), the degradation in HBNs sensory quality occurred, accompanied by a significant (p < 0.05) decrease in pH (5.95–5.01). These findings suggested that a moderate duration (4 h) of HBF pre-fermentation could effectively improve the quality of HBNs.

为了改善高原大麦面(HBNs)的质量,本研究采用植物乳杆菌对高原大麦面粉(HBF)进行预发酵。本研究测量了 HBNs 质量的变化、膳食纤维(DF)的水合特性和微观结构,以及 HBNs 中蛋白质的构象和交联。随着预发酵时间的延长(0-4 h),HBNs 的总体合格率从 7.83 提高到 8.27,HBNs 的硬度降低了 5.9%,回弹性提高了 4.5%。同时,共聚焦激光扫描显微镜(CLSM)显示,DF 的物理结构逐渐瓦解,导致其保水能力显著下降(p < 0.05)。此外,光谱分析显示,蛋白质中的β-匝含量持续上升(从 19.62% 升至 30.15%)。游离巯基和十二烷基硫酸钠提取蛋白(SDSEP)的含量分别下降了 18.1%和 4.12%。然而,随着预发酵时间的进一步延长(6-8 小时),HBNs 的感官质量发生了退化,同时 pH 值(5.95-5.01)显著下降(p < 0.05)。这些发现表明,适度的 HBF 预发酵时间(4 小时)可有效改善 HBN 的质量。
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引用次数: 0
Evaluation of anthocyanin-enriched wheat varieties (black, blue, purple) for enhanced antioxidant content and premium biscuit quality 评估富含花青素的小麦品种(黑、蓝、紫)以提高抗氧化剂含量和优质饼干品质
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-06-11 DOI: 10.1016/j.jcs.2024.103957
Usman Ali , Anita Kumari , Anjali Sharma , Mona Yadav , Satveer Kaur , Era Chaudhary , Bhawna Sheoran , Vandita Tiwari , Apoorv Tiwari , Pargat Singh , Ritu Nain , Ajay Goyal , Monika Garg

The anthocyanin-enriched wheat varieties were explored for their health benefits and development of antioxidant-rich biscuits. In the present study, around 35 advanced lines, showcasing different colors (7 white, 9 purple, 11 black, 8 blue), and robust yield potential under local climatic conditions were selected. The wheat lines were screened for yield, grain hardness, protein, dough quality, biscuit baking and sensory quality attributes. The two advanced black wheat lines (black-1 and black-6) showed low grain hardness indices (49.8 and 32.4) and protein content (9.1 and 8.2%). The dough obtained from these wheat lines exhibited excellent parameters including low hardness and high springiness. The black-1 (soft texture) and black-6 (very soft texture) lines exhibited superior biscuit-making quality with a high spread ratio (13.2 and 14), mirroring the positive controls {white-6 (vs)-15}, and surpassing the average (8.9) and negative controls {white-C3 (7.7) and white-C4 (8.4)}. Despite a reduction in anthocyanin content during baking, both lines demonstrated a 1.3-fold higher antioxidant activity compared to the control. These black wheat lines present significant potential for commercialization, offering an avenue to enhance the quality and nutritional profile of biscuits in the food industry.

研究人员探索了富含花青素的小麦品种,以了解其对健康的益处,并开发出富含抗氧化剂的饼干。本研究选择了约 35 个在当地气候条件下具有不同颜色(7 个白色、9 个紫色、11 个黑色、8 个蓝色)和强大产量潜力的先进品系。对这些小麦品系进行了产量、谷粒硬度、蛋白质、面团质量、饼干烘焙和感官质量属性的筛选。两个高级黑小麦品系(黑-1 和黑-6)的谷粒硬度指数(49.8 和 32.4)和蛋白质含量(9.1 和 8.2%)较低。从这些小麦品系中获得的面团表现出优异的参数,包括低硬度和高弹性。黑-1(质地柔软)和黑-6(质地非常柔软)品系表现出卓越的饼干制作质量,铺展率高(13.2 和 14),与阳性对照{白-6 (vs)-15}相同,超过了平均值(8.9)和阴性对照{白-C3 (7.7) 和白-C4 (8.4)}。尽管在烘焙过程中花青素含量有所减少,但与对照组相比,这两个品系的抗氧化活性都高出 1.3 倍。这些黑小麦品系具有巨大的商业化潜力,为食品工业提供了提高饼干质量和营养成分的途径。
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引用次数: 0
Oleogel for improving the texture and flavor in rice cooking: Preparation from natural wax and rice bran oil and characterization 用于改善米饭口感和风味的油凝胶:用天然蜡和米糠油制备油凝胶并进行表征
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-06-10 DOI: 10.1016/j.jcs.2024.103955
Lu Xu , Lin Liu , Yingge Wang , Shengmei Kang , Lili Cao , Min Pang

This study involved the preparation and characterization of oleogels from rice bran oil with different waxes and the potential application in improving the texture and flavor in rice cooking. The findings revealed a substantial improvement in rice texture with the addition of oleogel, particularly noteworthy at a 3% (w/w) incorporation of RBW (Rice Bran Wax). Under this condition, the hardness reached 15.75N, and stickiness measured 2.16N. The incorporation of 1–3% (w/w) oleogel significantly enhances the color vibrancy of rice during the cooking process, resulting in an increase in the L* value of the rice from 73.86 to 73.95-76.74. Sensory evaluation revealed higher scores for BW (Bee Wax) and RBW at 40.68 and 40.23 points, respectively. Microscopic structural analysis indicated that the incorporation of RBW oleogel led to rice samples with the smallest pores, contributing to a more refined internal pore structure in the grains. In the control rice, 7 aldehyde compounds were detected with a relative content of 43.44%. The addition of oleogel increased the number of aldehyde compounds to 9, with a relative content of 48.06%. This study expands the potential applications of oleogels in rice cooking, enriching flavor profiles and presenting opportunities for integration into the food industry.

这项研究涉及米糠油与不同蜡的油凝胶的制备和表征,以及在改善大米烹饪质地和风味方面的潜在应用。研究结果表明,加入油凝胶后,米饭的口感得到了显著改善,尤其是在米糠蜡(RBW)添加量为 3%(重量比)时。在此条件下,硬度达到 15.75N,粘性为 2.16N。在蒸煮过程中,加入 1-3%(重量比)的油凝胶可显著提高大米的色泽鲜艳度,使大米的 L* 值从 73.86 提高到 73.95-76.74。感官评估显示,BW(蜂蜡)和 RBW 的得分较高,分别为 40.68 分和 40.23 分。显微结构分析表明,加入 RBW 油凝胶后,大米样品的孔隙最小,从而使米粒的内部孔隙结构更加精细。对照组大米中检测到 7 种醛化合物,相对含量为 43.44%。加入油凝胶后,醛化合物的数量增加到 9 种,相对含量为 48.06%。这项研究拓展了油凝胶在大米烹饪中的潜在应用,丰富了大米的风味,为其融入食品工业提供了机会。
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引用次数: 0
Characteristics of whole wheat steamed bread production by two-stage fermentation procedure with Jiaozi as starter 以焦子为发酵剂的两阶段发酵法生产全麦馒头的特点
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-06-10 DOI: 10.1016/j.jcs.2024.103953
Zhijian Li , Tongyun Liu , Lulu Zhang

Given the beneficial role of biological acidification in improving the quality of cereal-based products, this study evaluated the applicability of Jiaozi as a starter for whole wheat dough fermentation and steamed bread quality improvement. During the first 12 h of dough fermentation, yeast and lactic acid bacteria (LAB) populations increased significantly, resulting in rapid carbohydrate consumption and acidification of the dough. The lactic/acetic acid molar ratio was approximately 3.3, which was within the recommended fermentation quotient (FQ). Compared with the unfermented dough and the dough fermented for 12 h, the dough fermented for 6 h had an improved protein network structure, and the steamed bread made from it also showed the best quality. At a low leavening temperature of 28 °C, satisfactory specific volume and height/diameter ratio were obtained. The dough with an inoculation amount of 50% of the fully fermented dough showed the highest CO2 production capacity. Compared with yeast, the whole wheat steamed bread produced by Jiaozi using the two-stage fermentation process showed higher specific volume, resistant starch (RS) content and protein digestibility. The results showed that the use of Jiaozi as a starter has great potential for application in improving the quality of whole wheat steamed bread.

鉴于生物酸化在改善谷物类产品质量方面的有益作用,本研究评估了娇子作为全麦面团发酵和馒头质量改善的启动剂的适用性。在面团发酵的最初 12 小时内,酵母和乳酸菌(LAB)数量显著增加,导致碳水化合物快速消耗和面团酸化。乳酸/醋酸摩尔比约为 3.3,符合推荐的发酵商数(FQ)。与未发酵面团和发酵 12 小时的面团相比,发酵 6 小时的面团蛋白质网络结构得到改善,用其制作的馒头质量也最好。在 28 °C 的低发酵温度下,获得了令人满意的比容和高/直径比。接种量为全发酵面团 50%的面团显示出最高的二氧化碳生产能力。与酵母相比,娇子采用两阶段发酵工艺生产的全麦馒头的比容、抗性淀粉(RS)含量和蛋白质消化率更高。结果表明,使用焦枝作为起始剂在提高全麦馒头质量方面具有很大的应用潜力。
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引用次数: 0
Zinc and selenium biofortification of sourdough breads with agronomically biofortified whole wheat flour 用农艺生物强化全麦面粉制作酸面包的锌和硒生物强化效果
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-31 DOI: 10.1016/j.jcs.2024.103952
Yaşar Karaduman , Aysel Gülbandılar , Arzu Akın , Seda Doğan , Erdinç Savaşlı

In this study, the possibility of enriching sourdough breads with agronomically biofortified whole wheat flour (BWWF) to remedy zinc and selenium deficiencies was investigated. For this purpose, two local sourdough breads were substituted with 25, 50, and 75% of BWWF and obtained with two yeast combinations. The zinc and selenium, physicochemical, nutritional, and product properties of breads have been determined. As replacement rates increased, the zinc content in the breads increased from 11.65 to 48.66 mg/kg and 8.56–45.98 mg/kg, and the selenium content increased from 48.5 to 266.8 μg/kg and 44.9–257.3 μg/kg. The zinc and selenium concentrations of breads that met the Recommended Daily Allowances (RDA) were roughly 25–40% at the 75 and 50% substitutes. Total phenolic content (TPC) and antioxidant activity increased in both breads. The SOG breads with the A-yeast combination had a substantially higher TPC. The hardness and volume of the breads decreased with the incorporation of BWWF bran, therefore lowering their sensory properties. However, at 25% and 50% substitution rates, this was limited. In general, the B-yeast combination of both breads had a higher content of amino acids. Biofortified sourdough breads could have a significant effect on eliminating zinc and selenium.

本研究调查了用农艺生物强化全麦面粉(BWWF)强化酸面团面包以弥补锌和硒缺乏的可能性。为此,用 25%、50% 和 75% 的生物强化全麦面粉替代了两种本地酸面团面包,并用两种酵母组合制作而成。对面包的锌和硒、理化、营养和产品特性进行了测定。随着替代率的增加,面包中的锌含量从 11.65 毫克/千克增加到 48.66 毫克/千克和 8.56-45.98 毫克/千克,硒含量从 48.5 微克/千克增加到 266.8 微克/千克和 44.9-257.3 微克/千克。在 75%和 50%的替代品中,符合每日推荐摄入量(RDA)的面包中锌和硒的含量大约为 25%-40%。两种面包的总酚含量(TPC)和抗氧化活性都有所增加。含有 A-酵母组合的 SOG 面包的总酚类物质含量要高得多。面包的硬度和体积随着 BWWF 麦麸的加入而降低,从而降低了面包的感官特性。不过,在替代率为 25% 和 50% 时,这种影响是有限的。总的来说,两种面包中的 B-酵母组合氨基酸含量更高。生物强化酸面包对锌和硒的消除有显著效果。
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引用次数: 0
Recent developments in the application of physical processing techniques for controlling browning in fresh wet noodles: A review 应用物理加工技术控制新鲜湿面条褐变的最新进展:综述
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-31 DOI: 10.1016/j.jcs.2024.103951
Mohammed Obadi, Qin Guo, Jun Sun, Bin Xu

Color is an important factor that affects the quality of noodles and consumer purchasing intentions and is an important indicator to measure the quality of noodles. However, the browning problem of fresh wet noodles has become an important factor affecting their quality and restricting their marketability. Therefore, inhibiting the browning of fresh wet noodles has always been one of the research hotspots for improving the quality of noodle products. Physical processing technology has gained widespread attention due to its advantages in terms of green safety and obvious inhibitory effects. Therefore, from this perspective, this review mainly elaborates on the noodle browning pathway and the factors affecting fresh wet noodles browning. Furthermore, the application status of physical processing technologies for controlling fresh wet noodles browning, mainly microwave, irradiation, ozone, and cold plasma, are reviewed and compared. Finally, the development direction to effectively inhibit fresh wet noodles browning was emphasized, providing a theoretical reference for improving fresh wet noodles browning.

色泽是影响面条质量和消费者购买意愿的重要因素,也是衡量面条质量的重要指标。然而,新鲜湿面条的褐变问题已成为影响其质量和制约其销售的重要因素。因此,抑制新鲜湿面条的褐变一直是提高面条产品质量的研究热点之一。物理加工技术因其绿色安全、抑制效果明显等优势而受到广泛关注。因此,从这个角度出发,本综述主要阐述了面条褐变的途径以及影响新鲜湿面条褐变的因素。此外,还对控制鲜湿面条褐变的物理加工技术,主要是微波、辐照、臭氧和冷等离子体的应用现状进行了综述和比较。最后,强调了有效抑制鲜湿面条褐变的发展方向,为改善鲜湿面条褐变提供了理论参考。
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引用次数: 0
Effects of heat exposure from late sowing on agronomic traits and the technological quality of hexaploid wheat 晚播受热对六倍体小麦农艺性状和技术质量的影响
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-28 DOI: 10.1016/j.jcs.2024.103950
Mike Sissons , Denise Pleming , Julian D. Taylor , Livinus Emebiri , Paul Eckermann , Nicholas C. Collins

Heat stress reduces wheat grain yield and affects quality traits relevant to trading and end use. In this study, 98 landraces and 152 elite hexaploid wheat varieties and breeding lines were trialled at two irrigated field sites in southern NSW to better understand heat stress responses and identify promising genotypes for heat tolerance breeding. Potential heat tolerance was defined using performance in heat-exposed late-sown (LS) plots relative to normal-sown (NS) plots, using a regression approach to calculate response indices uncorrelated with awn emergence time and performance per se in NS. The best individual responders for grain yield included genotypes from both Australia and Mexico. On average, lines selected in CIMMYT's High Temperature Wheat Yield Trials (HTWYT) performed well for yield per se in NS as well as for yield response to LS. CIMMYT lines previously selected for large grains under heat stress conditions performed well for grain size in NS and for LS responses of grain size and test weight. Dough peak resistance and time to peak resistance in a mixograph were increased by LS and these traits were positively correlated with grain protein and the occurrence of the Glu-D1d high molecular weight glutenin locus allele, respectively.

热胁迫会降低小麦谷物产量,并影响与贸易和最终用途相关的品质特征。本研究在新南威尔士州南部的两个灌溉田间试验了 98 个陆地小麦品种和 152 个六倍体小麦优良品种和育种品系,以更好地了解热胁迫反应,并确定有潜力的耐热育种基因型。利用回归法计算与芒出苗时间和正常播种(NS)地块本身的表现无关的反应指数,并根据受热晚播(LS)地块相对于正常播种(NS)地块的表现来定义潜在的耐热性。对谷物产量反应最好的个体包括澳大利亚和墨西哥的基因型。平均而言,在 CIMMYT 高温小麦产量试验(HTWYT)中选育的品系在 NS 中的单产以及对 LS 的产量响应方面表现良好。以前在热胁迫条件下选育的大粒 CIMMYT 品系在 NS 期的粒度以及 LS 期的粒度和测试重量方面表现良好。LS 增加了面团峰值阻力和在混合仪中达到峰值阻力的时间,这些性状分别与谷粒蛋白和 Glu-D1d 高分子量谷蛋白等位基因的出现呈正相关。
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引用次数: 0
Impact of rice friction milling on the bran layer and on kernel structure 摩擦碾米对米糠层和米粒结构的影响
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-21 DOI: 10.1016/j.jcs.2024.103936
Zilong Xu , Liu Yang , Xuan Xiao , Bo Cui , Jin Zhou , Qianmai Peng , Shaoyun Song , Houchang Pei , Yonglin Zhang

Moderate processing can improve the edible rice grain quality and nutrition. The grain structural-removal behavior was researched based on the macro and micro-structure analysis. Grain bran layer 3D profiling combined with iodine solution staining is applied for geometrical-structural analysis. Results show that rice grain can be divided into five structural region based on bran layer removal capacity, lateral > ventral > ventral groove > dorsal > dorsal groove. The relationship between grain geometric parameters and collision possibility is in-depth analyzed, results indicated large grain surface curvature leads to grain surface layer hard removal capacity. The region's bran layer thickness distribution affects its removal behavior and obey its removal capacity. The regional bran layer thickness order: dorsal(59.56 μm) > dorsal groove(50.62 μm) > ventral(43.91 μm) > ventral groove(42.83 μm) > lateral(37.08 μm). The bran layer micro-morphology impacts removal behavior, dorsal region with the groove structure, the bottom surface layer in grain groove structure can be removed when groove structure is damaged. The ventral groove depth is less than dorsal groove, showing better removal ability. The bran layer color in L*A*B*space shows strong correlation with remaining layer thickness, reflecting grain milling degree. Combined effects of grain geometry-parameters, bran layer thickness and micro-structure lead the final bran layer removal behavior. This study provides theoretical and practical basis for grain moderate processing.

适度加工可以改善食用米粒的品质和营养。在宏观和微观结构分析的基础上,研究了谷粒的结构去除行为。应用米糠层三维剖面图结合碘溶液染色进行几何结构分析。结果表明,米粒可根据糠层去除能力划分为五个结构区域:侧沟、腹沟、腹沟、背沟、背沟。深入分析了谷粒几何参数与碰撞可能性之间的关系,结果表明谷粒表面曲率大导致谷粒表层去除能力强。区域糠层厚度分布影响其去除行为,并服从其去除能力。区域糠层厚度顺序为:背面(59.56 μm)>;背沟(50.62 μm)>;腹面(43.91 μm)>;腹沟(42.83 μm)>;侧面(37.08 μm)。糠层的微观形态对去除行为有影响,背侧区域具有沟槽结构,当沟槽结构受到破坏时,谷物沟槽结构中的底表层可以被去除。腹侧沟槽深度小于背侧沟槽,显示出更好的去除能力。L*A*B* 空间中的糠层颜色与剩余层厚度有很强的相关性,反映了谷物的研磨程度。晶粒几何参数、麸层厚度和微观结构的综合影响导致了最终的麸层去除行为。这项研究为谷物适度加工提供了理论和实践依据。
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引用次数: 0
Impacts of the dose of nitrogen fertilizer applied to rice plants as top dressing on sake brewing 水稻顶部施肥时氮肥剂量对清酒酿造的影响
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-21 DOI: 10.1016/j.jcs.2024.103941
Takuji Miyamoto , Ikuhisa Nishida , Norikuni Ohtake , Dai Hirata

Applying nitrogen (N) fertilizer as top dressing to rice plants at the panicle formation stage affects the quality of rice grains. Our previous indoor experiment showed that application of a higher dose of top dressing N fertilizer to rice plants negatively affected the alcohol fermentation and yield of sake brewed from the grains. To further clarify the impacts of the N fertilizer dose, we performed a 2-year rice cultivation test in outdoor-installed containers with different doses of N fertilizer [1 (N1), 6 (N6), or 12 g N m−2 (N12)] applied as top dressing at the panicle formation stage. The frequency of grains with a white core and the protein content of the grains were 17%–26% lower and 8%–13% higher, respectively, in the N12 treatment than in the N1 treatment in both years. The yield of sake was consistently lower (1%–3%) from rice in the N12 treatment than from rice in the N1 treatment. The reduced sake yield and increased protein content in rice grains led to higher concentrations of amino acids in the sake brewed from rice in the N12 treatment. These results highlight the importance of fertilizer management in rice paddies for sake brewing.

在水稻植株的圆锥花序形成阶段施用氮肥会影响稻米的品质。我们之前的室内实验表明,对水稻植株施用较高剂量的氮肥会对酒精发酵和用稻米酿造的清酒产量产生负面影响。为了进一步明确氮肥剂量的影响,我们在室外安装的容器中进行了为期两年的水稻栽培试验,在水稻圆锥花序形成阶段施用不同剂量的氮肥[1(N1)、6(N6)或 12 g N m-2 (N12)]作为表层肥料。与 N1 处理相比,这两年 N12 处理中白芯谷粒的出现率和谷粒的蛋白质含量分别降低了 17%-26% 和提高了 8%-13% 。N12 处理的大米清酒产量一直比 N1 处理的大米清酒产量低(1%-3%)。清酒产量的降低和米粒中蛋白质含量的增加导致 N12 处理的大米酿造的清酒中氨基酸浓度更高。这些结果凸显了稻田肥料管理对清酒酿造的重要性。
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Journal of Cereal Science
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