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Combination of chemical modifications improves rice protein solubility 化学修饰组合可提高大米蛋白质的可溶性
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-21 DOI: 10.1016/j.jcs.2024.103939
Yi-Rong Yang , Wen-Kai Wu , Jhih-Ting Hsiao , Shu-Chen Hsieh , Fuu Sheu

Rice (Oryza sativa) is one of the most widely cultivated crops in the world, which makes it a promising source of non-animal protein. However, the utilization of RP in food is still limited due to its poor solubility. The present study modified RP through combinational methods and determined the changes in its solubility and functionality. Different combinations of three chemical modification methods, including deamidation, phosphorylation, and disulfide reduction, were employed. Disulfide reduction and following deamidation effectively improved the RP solubility from 1.29% to 51.45%, while if deamidation was applied before disulfide reduction the RP had the solubility increased to 40.46%. Phosphorylation showed little effect in enhancing RP solubility, rather it lowers the solubility when applied after the other two methods. Furthermore, the foaming properties, the water holding capacity, and the oil absorption capacity were also improved through the combinational methods. This study offers a potential avenue for enhancing the applicability of RP in various food applications.

水稻(Oryza sativa)是世界上种植最广泛的农作物之一,这使其成为一种前景广阔的非动物蛋白质来源。然而,由于可溶性差,RP 在食品中的利用仍然有限。本研究通过组合方法对 RP 进行改性,并测定其溶解性和功能性的变化。研究采用了三种化学改性方法的不同组合,包括脱氨基、磷酸化和二硫还原。二硫还原后再进行脱酰胺处理,可有效地将 RP 的溶解度从 1.29% 提高到 51.45%,而在二硫还原前进行脱酰胺处理,则可将 RP 的溶解度提高到 40.46%。磷酸化对提高 RP 溶解度的作用很小,在其他两种方法之后进行磷酸化反而会降低溶解度。此外,通过组合方法,发泡性能、持水能力和吸油能力也得到了改善。这项研究为提高 RP 在各种食品应用中的适用性提供了一条潜在的途径。
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引用次数: 0
Effects of sourdough-type fermentation and sorghum type on the techno-functional properties of the batter used for kisra, a fermented flatbread 酸面团类型发酵和高粱类型对发酵扁面饼(Kisra)所用面糊的技术功能特性的影响
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-21 DOI: 10.1016/j.jcs.2024.103937
Sami Sidahmed Ali, John RN. Taylor, Mohammad Naushad Emmambux

Techno-functional properties of six sorghum types were determined during spontaneous sourdough batter-type fermentation for Kisra production. All sorghum types showed a progressive decrease in pH to about 3.3 with fermentation for 40 h, accompanied by an increase in titratable acidity and free amino nitrogen. Fermentation increased pasting viscosity at 16 and 24 h, but decreased at 40 h due to starch hydrolysis. Protein hydrolysis as shown by SDS-PAGE leads to disaggregation of flour particles and the release of starch granules to increase the pasting viscosity. Thus, starch was more freely available to absorb water to paste and produce a higher viscosity from zero to 40 h. The decrease in pasting viscosity from 24 h to 40 h of fermentation was related to starch hydrolysis, as there was a reduction in total starch in the batter and pitting of starch granules, as shown by scanning electron microscopy. Fermentation had a more significant effect on techno-functional properties than sorghum types. Low protein and high tannin contents among the sorghum type also showed higher pasting viscosity. The techno-functional changes of sorghum batter during fermentation, especially the high pasting viscosity, might be helpful for structure design of kisra and other gluten-free sorghum-based products.

研究人员测定了六种高粱在自发酸面团发酵过程中的技术功能特性。所有类型的高粱在发酵 40 小时后,pH 值逐渐下降到 3.3 左右,可滴定酸度和游离氨基酸氮也随之增加。发酵在 16 和 24 小时后会增加糊化粘度,但在 40 小时后会因淀粉水解而降低。SDS-PAGE 显示,蛋白质水解会导致面粉颗粒分解,释放出淀粉颗粒,从而增加糊状粘度。因此,从零到 40 小时内,淀粉可更自由地吸水成糊状,并产生更高的粘度。发酵 24 小时到 40 小时内,糊状粘度的降低与淀粉水解有关,因为扫描电子显微镜显示,面糊中的总淀粉减少,淀粉颗粒出现点蚀。与高粱类型相比,发酵对技术功能特性的影响更为显著。蛋白质含量低和单宁酸含量高的高粱还表现出较高的糊化粘度。高粱面糊在发酵过程中的技术功能变化,尤其是高糊化粘度,可能有助于基斯拉和其他无麸质高粱产品的结构设计。
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引用次数: 0
Functional properties and structure of soluble dietary fiber obtained from rice bran with steam explosion treatment 通过汽爆处理从米糠中获得的可溶性膳食纤维的功能特性和结构
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-20 DOI: 10.1016/j.jcs.2024.103938
Xin-Yi Tian , Jian-Fu Liu , Cong-Cong Qiao , Zhuo Cheng , Na-Na Wu , Bin Tan

Rice bran was modified by steam explosion (SE) treatment to investigate the effect of different steam pressure (0.4, 0.8, 1.2, 1.6, and 2.0 MPa) with rice bran through 60 mesh and rice bran pulverization (60, 80, and 100 mesh) with the steam pressure of 1.2 MPa on the functional properties and structure of soluble dietary fiber (SDF) from SE-treated rice bran. SE pretreatment enhanced glucose and cholesterol adsorption capacity, water-holding capacity, oil-holding capacity, lipase inhibition capacity and anti-oxidation properties of SDF. However, functional properties of SDF were adversely affected when steam pressure was 2.0 MPa or crushing degree of rice bran was 100 mesh. The results showed that some glycosidic and hydrogen bonds were broken, resulting in a porous honeycomb structure and decreased polymerization degree of the crystalline regions and relative crystallinity in SDF from SE-treated rice bran. In general, the optimum condition for obtaining better properties of SDF was treated for rice bran at the steam pressure of 1.2 MPa and sieve size of 60 mesh. This finding emphasizes essential information on SE treatment of rice bran in modifying the properties of SDF, which is helpful to the development and utilization of SDF from SE-treated rice bran.

通过汽爆(SE)处理对米糠进行改性,研究不同蒸汽压力(0.4、0.8、1.2、1.6 和 2.0 兆帕)下米糠通过 60 目和蒸汽压力为 1.2 兆帕下米糠粉碎(60、80 和 100 目)对 SE 处理米糠中可溶性膳食纤维(SDF)的功能特性和结构的影响。SE 预处理提高了 SDF 的葡萄糖和胆固醇吸附能力、持水性、持油性、脂肪酶抑制能力和抗氧化性。然而,当蒸汽压力为 2.0 MPa 或米糠粉碎度为 100 目时,SDF 的功能特性受到不利影响。结果表明,一些糖苷键和氢键被破坏,导致多孔蜂窝结构,结晶区的聚合度和相对结晶度降低。总的来说,在蒸汽压力为 1.2 兆帕和筛孔尺寸为 60 目的条件下处理米糠,是获得性能更佳的 SDF 的最佳条件。这一发现强调了 SE 处理米糠在改变 SDF 性能方面的重要信息,有助于开发和利用 SE 处理米糠的 SDF。
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引用次数: 0
Relationship between the physicochemical properties and amylose content of rice starch in rice varieties with the same genetic background 具有相同遗传背景的水稻品种中水稻淀粉的理化性质与直链淀粉含量之间的关系
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-14 DOI: 10.1016/j.jcs.2024.103932
Hui Xu , Shunqian Xu , Yang Xu , Yanjie Jiang , Ting Li , Xinxia Zhang , Jie Yang , Li Wang

This study analyzed six mutant rice samples from uniform genetic and planting backgrounds with amylose contents ranging from 4.21% to 17.52%. The physicochemical, structural and functional properties of the extracted starches were investigated and their relationships were analyzed. No significant changes were observed in the morphology, granule size, amylopectin chain-length distribution, and crystalline type of rice starches. The average hydrodynamic radius (Rh), relative crystallinity (RC), the ratio of 1045 cm−1/1022 cm−1 (R1045/1022), and lamellar peak intensity (Imax) were significantly correlated with amylose content, impacting the digestion properties. A higher degree of order in starch structure was found to impede its digestion progress by affecting its physicochemical characteristics. The decreased onset temperature and increased gelatinization temperature range indicated increased crystalline structure instability, decreasing peak time and pasting temperature. Furthermore, there were notable reductions in starch digestion rate (k) and rapidly digestible starch content (RDS) as amylose content increased, despite no significant change in the final digestion percentage. The k exhibited a positive correlation with Rh, while there was a positive association between RC, R1045/1022, and Imax with RDS. These findings can provide valuable guidance for rice breeders in developing rice varieties with specific functional attributes.

本研究分析了六个突变水稻样本,这些样本具有统一的遗传和种植背景,直链淀粉含量从 4.21% 到 17.52% 不等。研究了提取淀粉的物理化学、结构和功能特性,并分析了它们之间的关系。结果表明,水稻淀粉的形态、颗粒大小、直链淀粉链长分布和结晶类型均无明显变化。平均流体力学半径(Rh)、相对结晶度(RC)、1045 cm-1/1022 cm-1 比值(R1045/1022)和片层峰强度(Imax)与直链淀粉含量显著相关,影响消化特性。研究发现,淀粉结构的有序度越高,就越会影响其理化特性,从而阻碍其消化进程。开始温度的降低和糊化温度范围的扩大表明结晶结构的不稳定性增加,峰值时间和糊化温度降低。此外,随着淀粉含量的增加,淀粉消化率(k)和快速消化淀粉含量(RDS)明显下降,尽管最终消化率没有显著变化。k 与 Rh 呈正相关,而 RC、R1045/1022 和 Imax 与 RDS 呈正相关。这些发现可为水稻育种者开发具有特定功能属性的水稻品种提供有价值的指导。
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引用次数: 0
Insight into elevated quality of whole millet (Pennisetum glaucum) cake through the process of extrusion 通过挤压工艺提高全小米(Pennisetum glaucum)饼质量的启示
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-13 DOI: 10.1016/j.jcs.2024.103931
Xinyu Zhang , Zijian Wu , Jinrong Wang , Ni Bian , Pei Zhao , Naxin Sun , Jinyu Chen

The practice of extrusion treatment has gained widespread adoption in the food industry. This treatment notably improved the quality of whole millet cake and the underlying mechanism was investigated. The results showed that millet extruded at 14% moisture could enhance the quality of whole millet cake by reducing hardness and increasing springiness, specific volume, and height-to-diameter ratio. Moreover, the improvement effect became more pronounced as the extrusion temperature reached 170 °C. However, it was worth noting that excessive moisture (18%) and high temperature (170 °C) during extrusion led to the highest gelatinization degree, resulting in a sticky cake batter that fails to form a soft cake during baking. In contrast, extrusion at low moisture (14%) and high temperature (170 °C) promoted starch degradation and formation of more starch complexes. These effects reduced stickiness while increasing the elasticity of the cake batter. This, in turn, contributes to the superior quality of the cake prepared using extruded millet flour at 14% moisture and 170 °C. The formation of starch complexes played a crucial role in enhancing the elasticity of the cake batter and overall cake quality, as they acted as supports within the cake batter matrix.

挤压处理的做法已在食品工业中得到广泛采用。这种处理方法显著提高了全小米饼的质量,并对其机理进行了研究。结果表明,在 14% 水分条件下挤压的小米可通过降低硬度、增加弹性、比容和高径比来提高全小米饼的质量。此外,当挤压温度达到 170 ℃ 时,改善效果更加明显。但值得注意的是,挤压过程中水分过高(18%)和温度过高(170 °C)会导致糊化度最高,从而使蛋糕糊变得粘稠,在烘焙过程中无法形成柔软的蛋糕。相反,在低水分(14%)和高温(170 °C)条件下挤压会促进淀粉降解,形成更多的淀粉复合物。这些作用在增加蛋糕糊弹性的同时降低了粘性。反过来,这也是使用水分为 14% 和温度为 170 °C 的挤压小麦粉制备的蛋糕质量上乘的原因。淀粉复合物的形成对提高蛋糕糊的弹性和蛋糕的整体质量起着至关重要的作用,因为它们在蛋糕糊基质中起着支撑作用。
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引用次数: 0
Enrichment of bread with soluble and insoluble rice bran dietary fibers: A comparative study 在面包中添加可溶性和不可溶性米糠膳食纤维:比较研究
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-01 DOI: 10.1016/j.jcs.2024.103927
Kefan Ouyang , Qin Tao , Hexiang Xie, Wenyu Wang, Wenyi Shi, Qianqian Shi, Hua Xiong, Qiang Zhao

Rice bran dietary fiber (RBDF) is a byproduct of rice milling that has potential applications in breadmaking. This study investigated the effects of adding RBDF at different soluble and insoluble fractions on bread quality and storage properties. RBDF was separated into soluble (RBSDF) and insoluble (RBIDF) fractions and added to the bread at various concentrations. The results showed that adding an appropriate amount of RBSDF (6%) improved the texture, specific volume, sensory evaluation, and shelf life of bread while adding too much RBSDF (12%) or RBIDF (>8%) reduced the quality of bread and increased its hardening and moisture loss during storage. The bread quality was improved when the addition of RBSDF and RBIDF were 6% and 5%, respectively, which could enhance the fiber intake, meeting the diversified dietary needs of consumers.

米糠膳食纤维(RBDF)是碾米的副产品,在面包制作中具有潜在的应用价值。本研究调查了添加不同可溶性和不可溶性部分的米糠膳食纤维对面包质量和储存特性的影响。将 RBDF 分离成可溶性(RBSDF)和不可溶性(RBIDF)部分,并以不同浓度添加到面包中。结果表明,添加适量的 RBSDF(6%)可改善面包的质地、比容、感官评价和保质期,而添加过多的 RBSDF(12%)或 RBIDF(8%)则会降低面包的质量,并增加其在贮藏过程中的硬化和水分损失。当 RBSDF 和 RBIDF 的添加量分别为 6% 和 5%时,面包的质量得到改善,从而提高了纤维的摄入量,满足了消费者多样化的膳食需求。
{"title":"Enrichment of bread with soluble and insoluble rice bran dietary fibers: A comparative study","authors":"Kefan Ouyang ,&nbsp;Qin Tao ,&nbsp;Hexiang Xie,&nbsp;Wenyu Wang,&nbsp;Wenyi Shi,&nbsp;Qianqian Shi,&nbsp;Hua Xiong,&nbsp;Qiang Zhao","doi":"10.1016/j.jcs.2024.103927","DOIUrl":"https://doi.org/10.1016/j.jcs.2024.103927","url":null,"abstract":"<div><p>Rice bran dietary fiber (RBDF) is a byproduct of rice milling that has potential applications in breadmaking. This study investigated the effects of adding RBDF at different soluble and insoluble fractions on bread quality and storage properties. RBDF was separated into soluble (RBSDF) and insoluble (RBIDF) fractions and added to the bread at various concentrations. The results showed that adding an appropriate amount of RBSDF (6%) improved the texture, specific volume, sensory evaluation, and shelf life of bread while adding too much RBSDF (12%) or RBIDF (&gt;8%) reduced the quality of bread and increased its hardening and moisture loss during storage. The bread quality was improved when the addition of RBSDF and RBIDF were 6% and 5%, respectively, which could enhance the fiber intake, meeting the diversified dietary needs of consumers.</p></div>","PeriodicalId":15285,"journal":{"name":"Journal of Cereal Science","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140825004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Demarcating quality improvement mechanisms in fresh yellow alkaline noodles: Insights into water-solid interaction, gluten network development, and starch properties during noodles resting 确定新鲜黄碱面的质量改进机制:面条静置过程中水固相互作用、面筋网络发展和淀粉特性的启示
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-01 DOI: 10.1016/j.jcs.2024.103935
Junkui Li, Yanan Shi, Yajing Qi, Tabussam Tufail, Bin Xu

This work explored, for the first time, the impact of noodles resting on the edible quality of fresh yellow alkaline noodles and demonstrated the promotion mechanism through analysis of physicochemical properties of principal components and microstructure of the noodles. According to the results, after resting for 18 h, fresh noodles exhibited reduced cooking loss and incresed water absorption. The chewiness and hardness of cooked noodles increased by 40.34% and 18.52%, respectively. An increase in the proportion of weakly bound water in the rested noodles was observed. indicating a more even distribution of moisture in noodles. Furthermore, a stronger gluten network structure and more organized secondary structure of gluten proteins were noted. The analysis of gluten network microstructure revealed a shorter average gluten network length, average protein width, higher branching rate, and lower terminal rate. Additionally, a higher peak viscosity of starch was found in the rested noodles.

本研究首次探讨了面条静置对新鲜黄碱面食用品质的影响,并通过分析面条主要成分的理化性质和微观结构,证明了面条静置对黄碱面食用品质的促进机制。结果表明,新鲜面条静置 18 小时后,蒸煮损失减少,吸水率增加。熟面条的嚼劲和硬度分别增加了 40.34% 和 18.52%。静置面条中弱结合水的比例增加,表明面条中的水分分布更均匀。此外,面筋网络结构更强,面筋蛋白的二级结构更有序。面筋网络微观结构分析表明,面筋网络平均长度、平均蛋白宽度更短,分支率更高,末端率更低。此外,静置面条的淀粉峰值粘度更高。
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引用次数: 0
Special bakery products - Acrylamide formation and bread quality are influenced by potato addition 特殊烘焙食品 - 添加马铃薯会影响丙烯酰胺的形成和面包质量
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-01 DOI: 10.1016/j.jcs.2024.103926
Jagoda Swiacka , Laura Kima , Alexander Voß , Sandra Grebenteuch , Sascha Rohn , Mario Jekle

Especially high levels of asparagine, like those present in potatoes, combined with reducing sugars, lead to increased acrylamide formation. As the influence of potato addition on acrylamide and baking quality in breads has not been examined yet, we aimed to investigate this area and prepared breads with potato flakes (7.5–22.5 g/100 g flour) at different moisture levels as a common mitigation approach (56.5–79.1 g/100 g flour). Analyses included reducing sugars (HPAEC–PAD), asparagine (HPLC) in dough, acrylamide (HPLC-MS/MS) in crumbs and crusts and baking quality measures (dimensions, baking yield, specific volume, lightness, browning index, firmness, crumb and crust moisture, crust-to-crumb ratio, crust thickness, pore analysis). High acrylamide concentrations were measured in crusts, reaching 292.3 ± 16.2 μg/kg, or 91.8 ± 5.3 μg/kg in bread. Significant (p < 0.001) linear correlations were identified between crust acrylamide and asparagine (r = 0.97), glucose (r = 0.85) and maltose (r = 0.92) in doughs, and also potato flakes addition (r = 0.95), surface browning index (r = 0.90) and bread lightness (r = −0.97). Summarizing, potato flakes addition led to higher precursor levels, while water addition influenced the reaction kinetics. Both parameters affected the quality of the bread significantly.

尤其是高水平的天门冬酰胺(如马铃薯中的天门冬酰胺)与还原糖相结合,会导致丙烯酰胺的形成增加。由于尚未研究过添加马铃薯对面包中丙烯酰胺和烘焙质量的影响,我们旨在调查这一领域,并以不同水分含量(56.5-79.1 克/100 克面粉)的马铃薯片(7.5-22.5 克/100 克面粉)作为常见的缓解方法,制备面包。分析包括还原糖(HPAEC-PAD)、面团中的天门冬酰胺(HPLC)、面包屑和面包皮中的丙烯酰胺(HPLC-MS/MS)以及烘焙质量指标(尺寸、烘焙产量、比容、光亮度、褐变指数、紧实度、面包屑和面包皮水分、面包皮与面包屑比率、面包皮厚度、孔隙分析)。在面包皮中测得的丙烯酰胺浓度较高,达到 292.3 ± 16.2 μg/kg,在面包中为 91.8 ± 5.3 μg/kg。面团中的丙烯酰胺与天冬酰胺(r = 0.97)、葡萄糖(r = 0.85)和麦芽糖(r = 0.92),以及马铃薯片添加量(r = 0.95)、表面褐变指数(r = 0.90)和面包亮度(r = -0.97)之间存在显著的线性相关(p < 0.001)。总之,马铃薯片的添加会导致前体水平升高,而水的添加会影响反应动力学。这两个参数对面包质量的影响都很大。
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引用次数: 0
The effects of different drying methods on the flavor profile of wheat germ using E-nose and GC-IMS 利用电子鼻和气相色谱-质谱分析不同干燥方法对小麦胚芽风味特征的影响
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-01 DOI: 10.1016/j.jcs.2024.103930
Yang Zhong, Gexing Zhang, Fangqin Zhang, Shuting Lin, Mei Wang, Yue Sun, Jin Liang, Xueling Li

The impact of hot-air drying (HA) and microwave-hot-air drying (MHA) on aroma components and free amino acids (FAAs) in wheat germ (WG) were investigated.

It was found that drying treatment improved the rancidity odor of WG, and MHA is better than HA. A total of 56 volatile compounds were identified in WG following different treatments, with 20 compounds identified as key differential compounds, and 13 key compounds were further screened based on the relative odor activity value (ROAV) > 1. Compared with HA, MHA accelerated the conversion of alcohols to aldehydes in WG, resulting in a more intense aroma and enhanced freshness and sweetness, but with an increase in bitter amino acids. Glutamic acid (Glu) exhibited a negative correlation with five aroma substances, while lysine (Lys) and arginine (Arg) were significantly negatively correlated with six and four aroma substances, respectively. The above results indicate that 2 types of drying have an impact on WG flavours, these findings offer valuable insights for the development and utilization of wheat germ, providing a reference for further exploration in this field.

研究了热风干燥(HA)和微波-热风干燥(MHA)对小麦胚芽(WG)中香气成分和游离氨基酸(FAA)的影响。研究发现,干燥处理可改善小麦胚芽的酸败气味,MHA的效果优于HA。与 HA 相比,MHA 加快了小麦胚芽中醇向醛的转化,使小麦胚芽的香气更浓郁,新鲜度和甜味增强,但苦味氨基酸增加。谷氨酸(Glu)与 5 种香气物质呈负相关,而赖氨酸(Lys)和精氨酸(Arg)分别与 6 种和 4 种香气物质呈显著负相关。上述结果表明,两种干燥方式对 WG 香味都有影响,这些发现为开发和利用小麦胚芽提供了有价值的见解,为这一领域的进一步探索提供了参考。
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引用次数: 0
Analysis of the effect of oat β-glucan on gut microbiota and clarification of their interaction relationship 燕麦β-葡聚糖对肠道微生物群的影响分析及其相互作用关系的澄清
IF 3.8 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-05-01 DOI: 10.1016/j.jcs.2024.103921
Junying Bai , Chuan Zhang , Jie Cui , Mingcong Fan , Yan Li , Linhua Huang , Li Wang

Oat β-glucan is catabolized by gut microbiota, however, the interaction relationship between β-glucan and gut microbiota is unclear. We aim to investigate the primary intestinal microbial species responsible for oat β-glucan degradation and explore their interaction. The result of in vitro fermentation showed that β-glucan significantly altered gut microbiota composition and primary microbial species responding to β-glucan was Lactobacillus. A total of 4 strains of Lactobacillus murinus were in vitro obtained and inoculated in the medium containing β-glucan as the sole carbon source to verify their association with β-glucan, and result showed that 4 strains of L. murinus could indeed degrade β-glucan. Genome analysis suggested that 4 strains of L. murinus contained abundant carbohydrate enzymes-encoding genes, among which genes encoding glycoside hydrolases family 1 (GH1) were most likely associated with β-glucan degradation. Further analysis revealed that GH1 could bind to β-glucan units through hydrogen bonding. Our study provides a novel insight into the research on interplay between dietary components and gut microbiota, and strategy for the exploration of potential carbohydrate hydrolase from gut microbiota for cereal β-glucan processing.

燕麦β-葡聚糖会被肠道微生物群分解,但β-葡聚糖与肠道微生物群之间的相互作用关系尚不清楚。我们的目的是研究负责燕麦β-葡聚糖降解的主要肠道微生物种类,并探讨它们之间的相互作用。体外发酵结果表明,β-葡聚糖显著改变了肠道微生物群的组成,而对β-葡聚糖做出反应的主要微生物种类是乳酸杆菌。为了验证乳酸杆菌与β-葡聚糖的关系,研究人员在体外获得了4株鼠乳酸杆菌,并将其接种到以β-葡聚糖为唯一碳源的培养基中,结果表明4株鼠乳酸杆菌确实能降解β-葡聚糖。基因组分析表明,4株L. murinus含有丰富的碳水化合物酶编码基因,其中编码糖苷水解酶家族1(GH1)的基因最有可能与β-葡聚糖降解有关。进一步分析表明,GH1 可通过氢键与β-葡聚糖单元结合。我们的研究为膳食成分与肠道微生物群之间相互作用的研究提供了新的视角,并为探索肠道微生物群中潜在的碳水化合物水解酶在谷物β-葡聚糖加工中的作用提供了策略。
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引用次数: 0
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Journal of Cereal Science
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