Aims: Comparison of the smear layer-removing capacity of several final irrigants (Er, Cr: YSGG laser (ECL), zinc phthalocyanine (TM-ZnPc), titanium: sapphire laser (Ti)) employed in the apical third of radicular dentin to and ethylenediamine tetraacetic acid (EDTA). Material and Methods: The chamber was opened, and 15 K file established canal patency. TruNatomy prime shaping files treated root canals. Root canal disinfectant was 3 mL of 2.25 NaOCl between each file. After disinfection, the teeth were randomly divided into four groups (n = 10). Groups 1–4:17% EDTA, ECL, Ti: sapphire laser, and TM-ZnPc. The root was separated by chiseling longitudinal grooves on the mesial and distal surfaces. SEM magnification at 5000X was used to evaluate smear layer removal based on dentinal tubule patency in the apical one-third of root canals using Gutman et al. grading system. IBM-SPSS 23.0 analyzed data. One-way ANOVA and Tukey’s post hoc test compared groups. p =0.05. Results: Group-2 (NaOCl+ECL) specimens removed the most smear layer. Group-4 (NaOCl+TM-ZnPc) removed the least smear layer. Group-1 (NaOCl+EDTA), Group-2, and Group-3 (NaOCl+Ti sapphire laser) showed equivalent smear layer removal efficacy. Conclusion: Er: Cr, YSGG, and Ti: sapphire laser with sodium hypochlorite has the potential to remove the apical third smear layer.
{"title":"Efficacy of Er.Cr.YSGG Laser, Ti Sapphire Laser and Zinc Pthalocyanine (TM ZnPc) Apical Root Canal Treatment on Smear Layer Removal. A Scanning Electron Microscopy Study","authors":"M. Alkahtany, K. Almadi","doi":"10.1166/jbt.2023.3321","DOIUrl":"https://doi.org/10.1166/jbt.2023.3321","url":null,"abstract":"Aims: Comparison of the smear layer-removing capacity of several final irrigants (Er, Cr: YSGG laser (ECL), zinc phthalocyanine (TM-ZnPc), titanium: sapphire laser (Ti)) employed in the apical third of radicular dentin to and ethylenediamine tetraacetic acid (EDTA). Material and Methods: The chamber was opened, and 15 K file established canal patency. TruNatomy prime shaping files treated root canals. Root canal disinfectant was 3 mL of 2.25 NaOCl between each file. After disinfection, the teeth were randomly divided into four groups (n = 10). Groups 1–4:17% EDTA, ECL, Ti: sapphire laser, and TM-ZnPc. The root was separated by chiseling longitudinal grooves on the mesial and distal surfaces. SEM magnification at 5000X was used to evaluate smear layer removal based on dentinal tubule patency in the apical one-third of root canals using Gutman et al. grading system. IBM-SPSS 23.0 analyzed data. One-way ANOVA and Tukey’s post hoc test compared groups. p =0.05. Results: Group-2 (NaOCl+ECL) specimens removed the most smear layer. Group-4 (NaOCl+TM-ZnPc) removed the least smear layer. Group-1 (NaOCl+EDTA), Group-2, and Group-3 (NaOCl+Ti sapphire laser) showed equivalent smear layer removal efficacy. Conclusion: Er: Cr, YSGG, and Ti: sapphire laser with sodium hypochlorite has the potential to remove the apical third smear layer.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"32 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139364523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hua Xin, Guang-Hao Tian, Nian-Ping Zhang, Hong-Jie Zhou, Guo-Zong Wang
The harvest of BMSCs has been relying on primary culture because construction of mesenchymal stem cell lines is difficult. The whole bone marrow method is a popular method to isolate and culture BMSCs. But the cell purity and extraction efficiency are not satisfactory using the traditional extraction method. The feasibility of an improved whole bone marrow method for extraction of human BMSCs was explored in our study. After multiple rounds of passage and culture, the cells maintained good morphology and proliferation. The characteristics of human BMSCs were demonstrated via in vitro trilineage-induced differentiation experiment and flow cytometry analysis. In conclusion, human BMSCs can be extracted and cultured successfully and simply using the improved whole bone marrow method.
{"title":"Isolation and Culture of Human Bone Marrow Mesenchymal Stem Cells by an Improved Method","authors":"Hua Xin, Guang-Hao Tian, Nian-Ping Zhang, Hong-Jie Zhou, Guo-Zong Wang","doi":"10.1166/jbt.2023.3308","DOIUrl":"https://doi.org/10.1166/jbt.2023.3308","url":null,"abstract":"The harvest of BMSCs has been relying on primary culture because construction of mesenchymal stem cell lines is difficult. The whole bone marrow method is a popular method to isolate and culture BMSCs. But the cell purity and extraction efficiency are not satisfactory using the traditional extraction method. The feasibility of an improved whole bone marrow method for extraction of human BMSCs was explored in our study. After multiple rounds of passage and culture, the cells maintained good morphology and proliferation. The characteristics of human BMSCs were demonstrated via in vitro trilineage-induced differentiation experiment and flow cytometry analysis. In conclusion, human BMSCs can be extracted and cultured successfully and simply using the improved whole bone marrow method.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"289 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139366174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study retrospectively analyzes the long-term clinical efficacy of single-stage arthroscopy-assisted surgery with limited incision in the treatment of knee dislocation (KD) with multiple ligament injuries (MLI). A total of 19 patients diagnosed with MLI at the First Affiliated Hospital of Guangxi Medical University and the 923th Hospital of the Joint Logistics Support Force of the People’s Liberation Army of China from March 2013 to May 2015 were included in this study. The functional levels were assessed using the Lysholm Knee Scoring Scale, International Knee Documentation Committee (IKDC) Subjective Knee Evaluation Form, Tegner Activity Score, and range of knee motion preoperatively. Non-parametric and Friedman tests were employed to analyze the surgical outcomes. The Lysholm scores increased from a preoperative value of 43.00 (95% confidence interval: 31.00, 50.00) to 88.00 (82.00, 95.00) at the post-operative follow-up of 10–36 months, and further improved to 90.00 (78.00, 98.00) after 8-year follow-up period. The IKDC scores increased from a preoperative value of 32.20 (28.70, 35.60) to 80.50 (60.90, 86.20) at 10–36 months post-operatively and further improved to 86.90 (69.00, 93.10) after 8-year follow-up period. The Tegner scores showed improvement from a preoperative value of 0.00 (0.00, 1.00) to 5.00 (3.00, 5.00) at 10–36 months postoperatively and to 4.0 (3.00, 5.00) after 8-year follow-up period. The ranges of active knee motion increased from preoperative 35.00 (30.00, 40.00) to 130.00 (120.00, 135.00) at 10–36 months and 8 years postoperative follow-up. In conclusion, it is recommended to adopt arthroscopy with limited incision for restoring knee joint stability and motor function.
{"title":"Long-Term Clinical Efficacy of Arthroscopy with Limited Incision in the Treatment of Knee Dislocation with Multiple Ligament Injuries","authors":"Yuqiang Huang, Wanan Qin, Baoqing Tang, Gejin Wei","doi":"10.1166/jbt.2023.3324","DOIUrl":"https://doi.org/10.1166/jbt.2023.3324","url":null,"abstract":"This study retrospectively analyzes the long-term clinical efficacy of single-stage arthroscopy-assisted surgery with limited incision in the treatment of knee dislocation (KD) with multiple ligament injuries (MLI). A total of 19 patients diagnosed with MLI at the First Affiliated Hospital of Guangxi Medical University and the 923th Hospital of the Joint Logistics Support Force of the People’s Liberation Army of China from March 2013 to May 2015 were included in this study. The functional levels were assessed using the Lysholm Knee Scoring Scale, International Knee Documentation Committee (IKDC) Subjective Knee Evaluation Form, Tegner Activity Score, and range of knee motion preoperatively. Non-parametric and Friedman tests were employed to analyze the surgical outcomes. The Lysholm scores increased from a preoperative value of 43.00 (95% confidence interval: 31.00, 50.00) to 88.00 (82.00, 95.00) at the post-operative follow-up of 10–36 months, and further improved to 90.00 (78.00, 98.00) after 8-year follow-up period. The IKDC scores increased from a preoperative value of 32.20 (28.70, 35.60) to 80.50 (60.90, 86.20) at 10–36 months post-operatively and further improved to 86.90 (69.00, 93.10) after 8-year follow-up period. The Tegner scores showed improvement from a preoperative value of 0.00 (0.00, 1.00) to 5.00 (3.00, 5.00) at 10–36 months postoperatively and to 4.0 (3.00, 5.00) after 8-year follow-up period. The ranges of active knee motion increased from preoperative 35.00 (30.00, 40.00) to 130.00 (120.00, 135.00) at 10–36 months and 8 years postoperative follow-up. In conclusion, it is recommended to adopt arthroscopy with limited incision for restoring knee joint stability and motor function.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"13 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139364790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: To compare the surface hardness of the nanohybrid resin composite with the other filler-containing resin composites. Material and Method: Abrasive wear properties of four types of resin composites: Harmonize (HM), Tetric N-Ceram (TNC), Filtek Z250 (Z250), and Filtek Z350 (Z350). Each composite had six 10 mm-diameter, 6 mm-thick cylindrical specimens for testing. The wear test involved subjecting the specimens to 800 cycles of abrasive wear under a 17 kg load. Surface hardness and weight loss were measured using a Vickers indenter micro-hardness tester after polishing. Surface morphology investigation was carried out using SEM on three specimens from each composite after the wear test. To identify significant differences in the wear characteristics of the resin composites, statistical analysis was conducted using one-way analysis of variance (ANOVA) and the Tukey HSD test (with α = 0.05). Results: The highest volumetric wear loss was observed in the Z350 XT nano-filled resin composite (71.19±2.49 mm3). The lowest was found in Z250 micro hybrid restorative material (36.33±1.57 mm3). The highest Vickers hardness was found in the Z250 micro-hybrid restorative material (111.24±2.1). Moreover, the lowest microhardness was found in tetric N ceram (TNC) (58.41±1.22). Conclusion: The wear resistance of micro-hybrid resin composite Z250 was found to be superior to that of other composite resins tested in the study.
{"title":"Examining Vicker Hardness and Wear Volume Minimization in Composite Resin with Different Filler Dimensions via Scanning Topography","authors":"Fahad H. Alkhudhairy, Y. AlFawaz, M. Bin-Shuwaish","doi":"10.1166/jbt.2023.3317","DOIUrl":"https://doi.org/10.1166/jbt.2023.3317","url":null,"abstract":"Aim: To compare the surface hardness of the nanohybrid resin composite with the other filler-containing resin composites. Material and Method: Abrasive wear properties of four types of resin composites: Harmonize (HM), Tetric N-Ceram (TNC), Filtek Z250 (Z250), and Filtek Z350 (Z350). Each composite had six 10 mm-diameter, 6 mm-thick cylindrical specimens for testing. The wear test involved subjecting the specimens to 800 cycles of abrasive wear under a 17 kg load. Surface hardness and weight loss were measured using a Vickers indenter micro-hardness tester after polishing. Surface morphology investigation was carried out using SEM on three specimens from each composite after the wear test. To identify significant differences in the wear characteristics of the resin composites, statistical analysis was conducted using one-way analysis of variance (ANOVA) and the Tukey HSD test (with α = 0.05). Results: The highest volumetric wear loss was observed in the Z350 XT nano-filled resin composite (71.19±2.49 mm3). The lowest was found in Z250 micro hybrid restorative material (36.33±1.57 mm3). The highest Vickers hardness was found in the Z250 micro-hybrid restorative material (111.24±2.1). Moreover, the lowest microhardness was found in tetric N ceram (TNC) (58.41±1.22). Conclusion: The wear resistance of micro-hybrid resin composite Z250 was found to be superior to that of other composite resins tested in the study.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"110 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139366096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Maawadh, T. Almohareb, K. Al Ahdal, A. AlShamrani, L. Al Deeb, A. Alrahlah
Aim: The purpose of the present study was to examine the effect of various disinfection regimes on the antimicrobial efficacy and bond integrity of CAD against two varieties of bacteria: lacto-bacilli and S. mutans. Material and methods: The investigation used SBS, SEM, elemental analysis, disinfection procedures, and failure mode analysis. Biofilm was grown on CAD samples. The specimens were randomly separated into five groups for disinfection: Group 1 received CHX treatment, Group 2 (Phycocyanain) PC treatment, Group 3 PC-PDT, Group 4 PC@ZrO2 NP-PDT, and Group 5 CO2 therapy. After disinfection, CFUs were measured, specimens were reconstituted, and SBS was tested using a universal testing machine (UTM). Stereomicroscope analyzed failures. Kruskal-Walis tested group survival rates. ANOVA and Post Hoc Tukey were used to calculate bond value mean and standard deviation for different disinfection techniques. All analyses used p<0.05. Results: The highest survival rate of S. mutans and Lactobacillus was found in group 2 CAD disinfected with PC only with no activation by PDT. The lowest survival rate of both bacteria was seen in Group 4: PC@ZrO2NP-PDT. Conclusion: PC@ZnO2NP-PDT demonstrated robust antibacterial activity against lactobacillus and S. mutans, albeit with lower bond values when compared to CHX and CO2.
{"title":"Phycocyanin-Incorporated Zirconium Dioxide Nanoparticles Activated by Photodynamic Therapy for Caries Affected Dentin Disinfection: “An Examination of Adhesive Bond Strength And Scanning Elemental Analysis”","authors":"A. Maawadh, T. Almohareb, K. Al Ahdal, A. AlShamrani, L. Al Deeb, A. Alrahlah","doi":"10.1166/jbt.2023.3320","DOIUrl":"https://doi.org/10.1166/jbt.2023.3320","url":null,"abstract":"Aim: The purpose of the present study was to examine the effect of various disinfection regimes on the antimicrobial efficacy and bond integrity of CAD against two varieties of bacteria: lacto-bacilli and S. mutans. Material and methods: The investigation used SBS, SEM, elemental analysis, disinfection procedures, and failure mode analysis. Biofilm was grown on CAD samples. The specimens were randomly separated into five groups for disinfection: Group 1 received CHX treatment, Group 2 (Phycocyanain) PC treatment, Group 3 PC-PDT, Group 4 PC@ZrO2 NP-PDT, and Group 5 CO2 therapy. After disinfection, CFUs were measured, specimens were reconstituted, and SBS was tested using a universal testing machine (UTM). Stereomicroscope analyzed failures. Kruskal-Walis tested group survival rates. ANOVA and Post Hoc Tukey were used to calculate bond value mean and standard deviation for different disinfection techniques. All analyses used p<0.05. Results: The highest survival rate of S. mutans and Lactobacillus was found in group 2 CAD disinfected with PC only with no activation by PDT. The lowest survival rate of both bacteria was seen in Group 4: PC@ZrO2NP-PDT. Conclusion: PC@ZnO2NP-PDT demonstrated robust antibacterial activity against lactobacillus and S. mutans, albeit with lower bond values when compared to CHX and CO2.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"34 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139364756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: This study examined the shear bond strength (SBS) and mode of failure of bioactive resin cement (BARC) and resin-modified glass ionomer cement (RMGIC) to dentin treated with Er, Cr: YSGG (ECrL) and femtosecond laser (FSL). Methods: 120 non-carious, non-fractured human molars without prior restorations were selected and processed. The teeth were grouped by surface conditioning. Groups 1 and 5 were untreated controls. Groups 2 and 6 had ECrL surface treatment, while Groups 3 and 7 had FSL. EDTA and Tetric N-Bond Universal conditioned Groups 4 and 8. Groups 1–4 (n = 15) employed BARC for bonding, while Groups 5–8 used RMGIC. A universal testing machine (UTM) tested shear bond strength, and a stereomicroscope studied the failure mode. Comparing findings required means, SDs, ANOVA, and Tukey’s post hoc test. Results: Group 1, without conditioning, has the lowest BARC-bonded SBS. In Group 4, EDTA+ Tetric N-Bond Universal-conditioned dentin bonded to BARC had the greatest SBS values. In the RMGIC-bonded groups, Group 5 without dentin conditioning had the lowest bond values, while EDTA+ Tetric NBond Universal-conditioned dentin had the greatest. Conclusion: EDTA and Tetric N-Bond Universal dentin conditioning improves RMGIC and BARC bond strength. This study’s conditioning methods boosted bond strength.
{"title":"Low-Level Laser Therapy of Er, Cr: YSGG and Femtosecond on Dentin Adhesion with Bioactive and Resin-Modified Glass Ionomer Cement","authors":"Fahad H. Alkhudhairy, Y. AlFawaz","doi":"10.1166/jbt.2023.3313","DOIUrl":"https://doi.org/10.1166/jbt.2023.3313","url":null,"abstract":"Aim: This study examined the shear bond strength (SBS) and mode of failure of bioactive resin cement (BARC) and resin-modified glass ionomer cement (RMGIC) to dentin treated with Er, Cr: YSGG (ECrL) and femtosecond laser (FSL). Methods: 120 non-carious, non-fractured human molars without prior restorations were selected and processed. The teeth were grouped by surface conditioning. Groups 1 and 5 were untreated controls. Groups 2 and 6 had ECrL surface treatment, while Groups 3 and 7 had FSL. EDTA and Tetric N-Bond Universal conditioned Groups 4 and 8. Groups 1–4 (n = 15) employed BARC for bonding, while Groups 5–8 used RMGIC. A universal testing machine (UTM) tested shear bond strength, and a stereomicroscope studied the failure mode. Comparing findings required means, SDs, ANOVA, and Tukey’s post hoc test. Results: Group 1, without conditioning, has the lowest BARC-bonded SBS. In Group 4, EDTA+ Tetric N-Bond Universal-conditioned dentin bonded to BARC had the greatest SBS values. In the RMGIC-bonded groups, Group 5 without dentin conditioning had the lowest bond values, while EDTA+ Tetric NBond Universal-conditioned dentin had the greatest. Conclusion: EDTA and Tetric N-Bond Universal dentin conditioning improves RMGIC and BARC bond strength. This study’s conditioning methods boosted bond strength.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"12 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acute kidney injury (AKI) manly occurred after sepsis and led to severe damage of the kidney. The present study was designed to explore the underlying mechanism of long non-coding RNA (lncRNA) SNHG14 in sepsis-induced AKI in In Vitro and In Vivo. The cecal ligation and puncture (CLP) rats was used as sepsis-induced AKI model for In Vivo studies and HK-2 cells with lipopolysaccharide (LPS) administration were used as cell model for In Vitro studies. Kidney tissues were stained by Hematoxylin and Eosin (H&E) for histological examination. The relative levels of SNHG14 and miR-145-5p was detected using qRT-PCR. The levels of serum cytokines, inflammatory factors, oxidative stress factors, apoptosis-related proteins were evaluated by ELISA and western blot. Apoptosis was detected using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. Compared with the sham group, the rats in the CLP group showed injured pathological morphology of kidney, high levels of serum Scr, and BUN, elevated contents of TNF-α, IL-6 and IL-1β, malondialdehyde (MDA) and apoptosis, but low SOD level in kidney tissues. However, all these changes were reversed by shRNA-SNHG14 administration or transfection In Vivo and In Vitro. SNHG14 level was upregulated, while miR-145-5p significantly decreased in kidney tissues of CLP rats and LPS-induced HK-2 cells. The target gene of miR-145-5p was SNHG14, verifying through luciferase reporter analysis. Further, miR-145-5p knockdown transfection significantly reversed the inhibition of SNHG14 interference on the secretion of inflammatory cytokines and apoptosis in LPS-induced HK-2 cells. SNHG14 silence can alleviate AKI in sepsis by upregulating the miR-145-5p expression. SNHG14 might be used as a potential therapeutic target for sepsis-induced AKI.
{"title":"Knockdown of Small Nucleolar RNA Host Gene 14 Alleviates Sepsis-Induced Acute Kidney Injury by Targeting miR-145-5p In Vitro and In Vivo","authors":"Gang Chen, Wenjing Wang, Min Zhu, Chuji Cheng","doi":"10.1166/jbt.2023.2627","DOIUrl":"https://doi.org/10.1166/jbt.2023.2627","url":null,"abstract":"Acute kidney injury (AKI) manly occurred after sepsis and led to severe damage of the kidney. The present study was designed to explore the underlying mechanism of long non-coding RNA (lncRNA) SNHG14 in sepsis-induced AKI in In Vitro and In Vivo. The cecal ligation and puncture (CLP) rats was used as sepsis-induced AKI model for In Vivo studies and HK-2 cells with lipopolysaccharide (LPS) administration were used as cell model for In Vitro studies. Kidney tissues were stained by Hematoxylin and Eosin (H&E) for histological examination. The relative levels of SNHG14 and miR-145-5p was detected using qRT-PCR. The levels of serum cytokines, inflammatory factors, oxidative stress factors, apoptosis-related proteins were evaluated by ELISA and western blot. Apoptosis was detected using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. Compared with the sham group, the rats in the CLP group showed injured pathological morphology of kidney, high levels of serum Scr, and BUN, elevated contents of TNF-α, IL-6 and IL-1β, malondialdehyde (MDA) and apoptosis, but low SOD level in kidney tissues. However, all these changes were reversed by shRNA-SNHG14 administration or transfection In Vivo and In Vitro. SNHG14 level was upregulated, while miR-145-5p significantly decreased in kidney tissues of CLP rats and LPS-induced HK-2 cells. The target gene of miR-145-5p was SNHG14, verifying through luciferase reporter analysis. Further, miR-145-5p knockdown transfection significantly reversed the inhibition of SNHG14 interference on the secretion of inflammatory cytokines and apoptosis in LPS-induced HK-2 cells. SNHG14 silence can alleviate AKI in sepsis by upregulating the miR-145-5p expression. SNHG14 might be used as a potential therapeutic target for sepsis-induced AKI.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"8 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Alrabiah, Hanan Alsunbul, Basil Almutairi, Mohammad Aljanakh, Khaled M. Alzahrani, T. Abduljabbar
Aims: To assess composite ceramics treated with Er, Cr: YSGG (ECL), Nd: YVO4, and Ti: Sapphire lasers to samples treated with hydrofluoric acid (HF) and silane (S) for shear bond strength (SBS) and surface roughness (Ra). Material and Methods: 60 disc-shaped HRC specimens. Surface conditioning techniques divided HRC-prepared discs into four groups. Samples in Group 1: HRC pretreated with normal 9.5% HF, Group 2: ECL, Group 3: Ti-Sapphire, Group 4: Nd: YVO4. The profilometer measured all ceramic discs’ average surface Ra. Universal testing machines tested the resin-ceramic interface SBS. Surface topography was assessed using SEM. Shear bond scores and Ra were compared using a one-way ANOVA. A Tukey’s post hoc test assessed several group comparisons. Results: Group 2 (Ti Sapphire Laser+S) samples scored highest in Ra and SBS. Group 4 Nd: YVO4+S had the lowest Ra and SBS values. HF 20 sec+S, (Ti Sapphire Laser+S), (ECL+S), and (Nd: YVO4+S) had comparable SBS and Ra values (p>0.05). Conclusion: Laser-conditioned hybrid ceramics Er, Cr: YSGG, Nd: YVO4, and Ti-Sapphire lasers have shear bond strength and surface roughness comparable to 9.6% hydrofluoric acid.
目的：评估用 Er、Cr：YSGG (ECL)、Nd: YVO4 和 Ti: Sapphire 激光处理的复合陶瓷与氢氟酸 (HF) 和硅烷 (S) 处理的样品的剪切结合强度 (SBS) 和表面粗糙度 (Ra)。材料与方法：60 个圆盘状 HRC 试样。 表面调节技术将热轧卷制备的圆盘分为四组。第 1 组：用普通 9.5% HF 预处理的 HRC，第 2 组：ECL，第 3 组：Ti-Sapphire，第 4 组：Nd: YVO4。轮廓仪测量了所有陶瓷盘的平均表面 Ra 值。万能试验机测试了树脂-陶瓷界面 SBS。使用 SEM 评估表面形貌。采用单因素方差分析比较剪切粘接得分和 Ra。采用 Tukey 后检验对几个组进行比较。结果如下第 2 组（钛蓝宝石激光+S）样品在 Ra 和 SBS 方面得分最高。第 4 组 Nd：YVO4+S 的 Ra 值和 SBS 值最低。HF 20 秒+S、（钛蓝宝石激光+S）、（ECL+S）和（钕：YVO4+S）的 SBS 和 Ra 值相当（p>0.05）。结论激光调节混合陶瓷 Er、Cr：YSGG、Nd：YVO4 和 Ti-Sapphire 激光混合陶瓷的剪切结合强度和表面粗糙度与 9.6% 的氢氟酸相当。
{"title":"Er, Cr: YSGG, Nd: YVO4, and Ti: Sapphire Lasers for Surface Treatment of Hybrid Ceramics on Bond Durability, Surface Texture, and Debonding to Resin Cement: A Scanning Electron Microscopy (SEM) Analysis","authors":"M. Alrabiah, Hanan Alsunbul, Basil Almutairi, Mohammad Aljanakh, Khaled M. Alzahrani, T. Abduljabbar","doi":"10.1166/jbt.2023.3318","DOIUrl":"https://doi.org/10.1166/jbt.2023.3318","url":null,"abstract":"Aims: To assess composite ceramics treated with Er, Cr: YSGG (ECL), Nd: YVO4, and Ti: Sapphire lasers to samples treated with hydrofluoric acid (HF) and silane (S) for shear bond strength (SBS) and surface roughness (Ra). Material and Methods: 60 disc-shaped HRC specimens. Surface conditioning techniques divided HRC-prepared discs into four groups. Samples in Group 1: HRC pretreated with normal 9.5% HF, Group 2: ECL, Group 3: Ti-Sapphire, Group 4: Nd: YVO4. The profilometer measured all ceramic discs’ average surface Ra. Universal testing machines tested the resin-ceramic interface SBS. Surface topography was assessed using SEM. Shear bond scores and Ra were compared using a one-way ANOVA. A Tukey’s post hoc test assessed several group comparisons. Results: Group 2 (Ti Sapphire Laser+S) samples scored highest in Ra and SBS. Group 4 Nd: YVO4+S had the lowest Ra and SBS values. HF 20 sec+S, (Ti Sapphire Laser+S), (ECL+S), and (Nd: YVO4+S) had comparable SBS and Ra values (p>0.05). Conclusion: Laser-conditioned hybrid ceramics Er, Cr: YSGG, Nd: YVO4, and Ti-Sapphire lasers have shear bond strength and surface roughness comparable to 9.6% hydrofluoric acid.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"9 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xu-Mei Cheng, Rui Ma, Yan-Bing Zhou, Tao Li, Sheng-Nan Lei, Xin Zheng, Hui-Xiao Zhang, Mei-Tian Liu, Jianye Zhou, Zhi-Qiang Li
Bone defect repair is a hot topic in tissue engineering, and the development of economical and effective bone scaffold materials to achieve the goal of repairing bone defects is an important task for researchers. In this experiment, fresh bovine cancellous bone was used as a xenogeneic bone material. After two high-temperature calcinations, β-tricalcium phosphate (β-TCP) material was obtained. An in-situ co-precipitation method was employed to prepare hydroxyapatite+graphene oxide (HA+GO) composite material. Then, the β-TCP material was immersed in the HA+GO composite material solution to prepare β-TCP+HA+GO composite material. The surface modification of β-TCP material with HA and GO improved the elastic modulus of the raw material. In vitro cell showed that the composite material was non-toxic, biocompatible, and promoted the proliferation of MC3T3-E1 cells on the β-TCP+HA+GO material. Finally, it was found that β-TCP+HA+GO composite material effectively stimulated the actin and mineralization behavior of MC3T3-E1 cells, and promoted the expression of bone-related factors such as Alkaline phosphatase (ALP), Collagen I (COLI), Osteocalcin (OCN), Bone Morphogenetic Protein-2 (BMP-2), and Bone Morphogenetic Protein-7 (BMP-7) in MC3T3-E1 cells In conclusion, β-TCP+HA+GO composite material can be applied in the biomedical field as a bone substitute or repair material.
{"title":"Preparation and Osteogenic Properties of β-TCP+HA+GO Composites","authors":"Xu-Mei Cheng, Rui Ma, Yan-Bing Zhou, Tao Li, Sheng-Nan Lei, Xin Zheng, Hui-Xiao Zhang, Mei-Tian Liu, Jianye Zhou, Zhi-Qiang Li","doi":"10.1166/jbt.2023.3310","DOIUrl":"https://doi.org/10.1166/jbt.2023.3310","url":null,"abstract":"Bone defect repair is a hot topic in tissue engineering, and the development of economical and effective bone scaffold materials to achieve the goal of repairing bone defects is an important task for researchers. In this experiment, fresh bovine cancellous bone was used as a xenogeneic bone material. After two high-temperature calcinations, β-tricalcium phosphate (β-TCP) material was obtained. An in-situ co-precipitation method was employed to prepare hydroxyapatite+graphene oxide (HA+GO) composite material. Then, the β-TCP material was immersed in the HA+GO composite material solution to prepare β-TCP+HA+GO composite material. The surface modification of β-TCP material with HA and GO improved the elastic modulus of the raw material. In vitro cell showed that the composite material was non-toxic, biocompatible, and promoted the proliferation of MC3T3-E1 cells on the β-TCP+HA+GO material. Finally, it was found that β-TCP+HA+GO composite material effectively stimulated the actin and mineralization behavior of MC3T3-E1 cells, and promoted the expression of bone-related factors such as Alkaline phosphatase (ALP), Collagen I (COLI), Osteocalcin (OCN), Bone Morphogenetic Protein-2 (BMP-2), and Bone Morphogenetic Protein-7 (BMP-7) in MC3T3-E1 cells In conclusion, β-TCP+HA+GO composite material can be applied in the biomedical field as a bone substitute or repair material.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"4 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Waleed M S Alqahtani, Salah A. Yousuf, Mohamed F. Metwally, Khaled M. Haggag, A. Barakat, Abdelrahman Alghamdi, Roua Mohammed Y. Almadani, Hajer Aiydh Hamed Alsalmi, Adnan Madani Mohammed Khamis
Objective: This study is the impact of resin composite and bio dentine cervical marginal elevation on vertical marginal gap using two CAD/CAM endo-crowns. Materials and Methods: 32 molars were employed, separated into two main groups: Group R, which included 16 molar teeth elevated distally with resin composite each; and Group B, which included 16 molar teeth elevated distally with dentine. The prepared teeth were then restored using endo-crown restorations. There were 2 equal subgroups for each main group (Vita MarkII and Lava Ultimate endo-crowns; n = 8). The samples underwent thermocycling (TC) after the restorations were bonded using dual-cure resin cement. A digital microscope was used to measure the vertical marginal gap. Results: A significant difference in the vertical marginal gap following thermocycling in all groups, except for the Resin composite. The polymer-based group demonstrated better marginal adaptation than other groups. The marginal accuracy produced by the marginal elevation approach, which involves placing a composite filling and dentine in the proximal box before the insertion of ceramic indirect restorations, is comparable to those of ceramics implanted in dentine without margin elevation. Conclusion: The margin elevation technique ensures that the margins created with these materials are as precise as those placed directly in dentin.
{"title":"Comparison of Resin Composite and Biodentine Cervical Marginal Elevation on the Marginal Gap of Two Computer-Aided Design/Computer-Aided Manufacture Endocrown Materials: In Vitro Study","authors":"Waleed M S Alqahtani, Salah A. Yousuf, Mohamed F. Metwally, Khaled M. Haggag, A. Barakat, Abdelrahman Alghamdi, Roua Mohammed Y. Almadani, Hajer Aiydh Hamed Alsalmi, Adnan Madani Mohammed Khamis","doi":"10.1166/jbt.2023.3312","DOIUrl":"https://doi.org/10.1166/jbt.2023.3312","url":null,"abstract":"Objective: This study is the impact of resin composite and bio dentine cervical marginal elevation on vertical marginal gap using two CAD/CAM endo-crowns. Materials and Methods: 32 molars were employed, separated into two main groups: Group R, which included 16 molar teeth elevated distally with resin composite each; and Group B, which included 16 molar teeth elevated distally with dentine. The prepared teeth were then restored using endo-crown restorations. There were 2 equal subgroups for each main group (Vita MarkII and Lava Ultimate endo-crowns; n = 8). The samples underwent thermocycling (TC) after the restorations were bonded using dual-cure resin cement. A digital microscope was used to measure the vertical marginal gap. Results: A significant difference in the vertical marginal gap following thermocycling in all groups, except for the Resin composite. The polymer-based group demonstrated better marginal adaptation than other groups. The marginal accuracy produced by the marginal elevation approach, which involves placing a composite filling and dentine in the proximal box before the insertion of ceramic indirect restorations, is comparable to those of ceramics implanted in dentine without margin elevation. Conclusion: The margin elevation technique ensures that the margins created with these materials are as precise as those placed directly in dentin.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"10 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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