Aim: The effect of three different veneering techniques (layering, press-over, and CAD/CAM techniques) on the fracture resistance of lithium disilicate crown (LDC). Material and Methods: Thirty lithium disilicate crowns were adhesively cemented on the standardized Epoxy die. LDC was fabricated according to the veneering materials and techniques into three groups (n = 10): group (LV) layering veneering technique, group (PV) pressed veneering technique, and group DV (CAD/CAM) technique. The specimen was artificially aged through dynamic loading and thermocycling. All specimens were tested for fracture resistance using compressive load. Descriptive statistics of frequency distribution mean and standard deviations were calculated and compared across different groups. ANOVA was used to evaluate the effect of the veneering technique on fracture resistance. Results: The highest load was demonstrated in the DV group (1057.26762±97.04401 N) and the lowest load was found in PV group (762.41229±102.56927 N). Similarly, the highest fracture resistance was observed in group DV (14.65171±1.34484 MPa), and the lowest was found in group PV group (10.56558±1.42141 MPa). Mean values of maximum loads and fracture resistance in veneers fabricated by digital, pressed, and layer veneering techniques showed a significant difference. Conclusion: The CAD/CAM veneered monolithic lithium disilicate crowns demonstrated superior fracture resistance compared to the lithium disilicate crowns fabricated by over-pressing and layering techniques.
{"title":"Effect of Different Veneering Techniques on the Fracture Resistance of Bioceramic Lithium Disilicate Ceramics Crowns","authors":"Ali Barakat, Mohammed S. Alomari","doi":"10.1166/jbt.2023.3281","DOIUrl":"https://doi.org/10.1166/jbt.2023.3281","url":null,"abstract":"Aim: The effect of three different veneering techniques (layering, press-over, and CAD/CAM techniques) on the fracture resistance of lithium disilicate crown (LDC). Material and Methods: Thirty lithium disilicate crowns were adhesively cemented on the standardized Epoxy\u0000 die. LDC was fabricated according to the veneering materials and techniques into three groups (n = 10): group (LV) layering veneering technique, group (PV) pressed veneering technique, and group DV (CAD/CAM) technique. The specimen was artificially aged through dynamic loading and thermocycling.\u0000 All specimens were tested for fracture resistance using compressive load. Descriptive statistics of frequency distribution mean and standard deviations were calculated and compared across different groups. ANOVA was used to evaluate the effect of the veneering technique on fracture resistance.\u0000 Results: The highest load was demonstrated in the DV group (1057.26762±97.04401 N) and the lowest load was found in PV group (762.41229±102.56927 N). Similarly, the highest fracture resistance was observed in group DV (14.65171±1.34484 MPa), and the lowest was found\u0000 in group PV group (10.56558±1.42141 MPa). Mean values of maximum loads and fracture resistance in veneers fabricated by digital, pressed, and layer veneering techniques showed a significant difference. Conclusion: The CAD/CAM veneered monolithic lithium disilicate crowns demonstrated\u0000 superior fracture resistance compared to the lithium disilicate crowns fabricated by over-pressing and layering techniques.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48089682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lamees Alssum, Maha Alghofaily, Mona Elsafadi, Jawahir Abuhaimed, Randa Almadhari, Nouf Alshibani, R. Al-Kattan, Amer Mahmood
Background and objectives: Myrrh (Commiphora molmol) is a natural resinous substance derived from the bark of the Commiphora molmol tree, which is native to Eastern Africa and the Arabian Peninsula. It has been used for thousands of years in traditional medicine for its well-known antimicrobial, analgesic, and anti-inflammatory properties. Recently, it has gained attention for its potential regenerative medicine applications. The aim of the current study was to evaluate the biocompatibility and mineralization potential of myrrh on human mesenchymal stem cells (hMSC). Methods: Myrrh solution (MS) was prepared from commercial organic myrrh resin. The hMSC cell line were exposed to nine different concentrations of MS and viability was assessed using the Alamar Blue assay. The mineralization potential of myrrh was evaluated using alkaline phosphatase (ALP) activity assay and Alizarin Red S (ARS) staining. Results: At concentrations lower than 15.6 ug/ml after 7 and 14 days of treatment, cell viability levels were not markedly different from the control indicating low cytotoxic effect of the MS on hMSC. ALP levels were higher in the MS experimental groups compared to the control group. The AZR results were consistent with the ALP levels and confirmed that MS promoted hMSC mineralization. Conclusions: These findings confirm the cellular biocompatibility and the mineralization potential of myrrh in hMSC cell lines in vitro.
{"title":"Biocompatibility and Mineralization Potential of Myrrh (Commiphora molmol) on Human Bone Marrow-Derived Mesenchymal Stem Cells","authors":"Lamees Alssum, Maha Alghofaily, Mona Elsafadi, Jawahir Abuhaimed, Randa Almadhari, Nouf Alshibani, R. Al-Kattan, Amer Mahmood","doi":"10.1166/jbt.2023.3282","DOIUrl":"https://doi.org/10.1166/jbt.2023.3282","url":null,"abstract":"Background and objectives: Myrrh (Commiphora molmol) is a natural resinous substance derived from the bark of the Commiphora molmol tree, which is native to Eastern Africa and the Arabian Peninsula. It has been used for thousands of years in traditional medicine\u0000 for its well-known antimicrobial, analgesic, and anti-inflammatory properties. Recently, it has gained attention for its potential regenerative medicine applications. The aim of the current study was to evaluate the biocompatibility and mineralization potential of myrrh on human mesenchymal\u0000 stem cells (hMSC). Methods: Myrrh solution (MS) was prepared from commercial organic myrrh resin. The hMSC cell line were exposed to nine different concentrations of MS and viability was assessed using the Alamar Blue assay. The mineralization potential of myrrh was evaluated using\u0000 alkaline phosphatase (ALP) activity assay and Alizarin Red S (ARS) staining. Results: At concentrations lower than 15.6 ug/ml after 7 and 14 days of treatment, cell viability levels were not markedly different from the control indicating low cytotoxic effect of the MS on hMSC. ALP levels\u0000 were higher in the MS experimental groups compared to the control group. The AZR results were consistent with the ALP levels and confirmed that MS promoted hMSC mineralization. Conclusions: These findings confirm the cellular biocompatibility and the mineralization potential of myrrh\u0000 in hMSC cell lines in vitro.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45619376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: This study aimed to construct a TDI-induced mouse model of asthma, and evaluate the potential effects and possible molecular mechanisms of Calycosin on airway inflammation and airway remodeling in mouse model. Material and methods: ELISA method was applied to detect the total serum IgE level and the inflammatory cytokine level in the bronchoalveolar lavage fluid. The total number of cells and the proportion of inflammatory cells in BALF were evaluated under an optical microscope. HE was employed to assess and score the infiltration of peritracheal and perivascular inflammatory cells in lung tissue, and PAS staining was used to assess the proportion of goblet cells in the airway epithelium and the thickness of airway epithelial reticular basement membrane in each group of mice. WB was used to detect the expressions of HMGB1 and a-SMA in cells. Immunofluorescence staining was used to detect the expressions of HMGB1 and a-SMA in 16HBEs. Results: The airway hyperresponsiveness of the Calycosin TDI asthma mice decreased, the inflammatory factors in BALF and the total serum IgE levels decreased, the airway epithelial goblet cell metaplasia and the thickness of the airway epithelial reticular basement membrane were improved, thus reducing the up-regulation of HMGB1 and a-SMA expression of 16HBES induced by TDI-HSA. Conclusion: In our study, in the TDI-induced mouse model of asthma, the administration of drug to inhibit the activation of AKT can reduce airway inflammation and airway remodeling. These findings have enriched the current understanding of Calycosin and provided a basis for future research. However, there are also some limitations: How does TDI activate the AKT signaling pathway? After the activation of the AKT pathway, the mechanism by which the expressions of HMGB1, α-SMA and Collagen-I were up-regulated has not been fully elucidated.
{"title":"Effect of Calycosin on Airway Inflammation and Airway Remodeling in Allergic Asthma Mouse Model","authors":"Li Huang, Mingjuan Zhang, Jin-rong Xiong","doi":"10.1166/jbt.2023.3291","DOIUrl":"https://doi.org/10.1166/jbt.2023.3291","url":null,"abstract":"Background: This study aimed to construct a TDI-induced mouse model of asthma, and evaluate the potential effects and possible molecular mechanisms of Calycosin on airway inflammation and airway remodeling in mouse model. Material and methods: ELISA method was applied\u0000 to detect the total serum IgE level and the inflammatory cytokine level in the bronchoalveolar lavage fluid. The total number of cells and the proportion of inflammatory cells in BALF were evaluated under an optical microscope. HE was employed to assess and score the infiltration of peritracheal\u0000 and perivascular inflammatory cells in lung tissue, and PAS staining was used to assess the proportion of goblet cells in the airway epithelium and the thickness of airway epithelial reticular basement membrane in each group of mice. WB was used to detect the expressions of HMGB1 and a-SMA\u0000 in cells. Immunofluorescence staining was used to detect the expressions of HMGB1 and a-SMA in 16HBEs. Results: The airway hyperresponsiveness of the Calycosin TDI asthma mice decreased, the inflammatory factors in BALF and the total serum IgE levels decreased, the airway epithelial\u0000 goblet cell metaplasia and the thickness of the airway epithelial reticular basement membrane were improved, thus reducing the up-regulation of HMGB1 and a-SMA expression of 16HBES induced by TDI-HSA. Conclusion: In our study, in the TDI-induced mouse model of asthma, the administration\u0000 of drug to inhibit the activation of AKT can reduce airway inflammation and airway remodeling. These findings have enriched the current understanding of Calycosin and provided a basis for future research. However, there are also some limitations: How does TDI activate the AKT signaling pathway?\u0000 After the activation of the AKT pathway, the mechanism by which the expressions of HMGB1, α-SMA and Collagen-I were up-regulated has not been fully elucidated.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43781798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yanpeng Xu, Jiahuan Li, Song Yu, Yan Chen, Zhixu He
The pathogenesis and the mechanism of orally administered propranolol in the treatment of hemangioma are unclear. In this study, we evaluated the changes of xenograft hemangioma in nude mice after intervention with estradiol and propranolol. Raf-1 and p-ERK expression in xenograft hemangiomas was assessed to evaluate their role in hemangioma proliferation and regression after treatment. A hemangioma xenograft model in nude mice was established. The successful xenograft specimens were selected and then randomized into control group, estradiol group and propranolol group. At the date of injection, and on day 7 and 21 after injection, the morphological changes of xenograft hemangiomas were visually characterized and imaged by light microscopy. The distribution and expression Raf-1 and p-ERK protein was determined by immunohistochemical detection. In control group, the xenografts increased gradually in volume, had a soft texture and their colors gradually turned red with observation of proliferation of endothelial cells and a capillary lumen that contained monolayer endothelial cells. In Estradiol group, the xenografts grew fast and increased significantly in volume, had a soft texture and their colors were dark red with a hyperplasia of endothelial cells, irregular volume, and deranged and compact endothelial cells. More capillary lumens and sinuses were also seen. Raf-1 and p-ERK expression in estradiol group was significantly increased (P < 0.05). In Propranolol group, the xenografts volume decreased, had a soft texture, and their colors turned gradually white with decreased number of proliferative endothelial cells. The vascular lumens, composed of endothelial cells, were larger, and some of them disappeared and were replaced by fibrous connective tissue and vascular adipose tissue. Raf-1 and p-ERK expression in propranolol group was lower than estradiol and control group (P < 0.05). In conclusion, Raf-1/ERK signaling pathway may be involved in hemangioma. Estrogen and propranolol may regulate the proliferation or regression of hemangioma through Raf-1/ERK signaling pathway.
{"title":"Role of Raf-1/ERK Signaling Pathway in Estradiol and Propranolol in the Intervention of Xenograft Hemangioma In Vivo","authors":"Yanpeng Xu, Jiahuan Li, Song Yu, Yan Chen, Zhixu He","doi":"10.1166/jbt.2023.3285","DOIUrl":"https://doi.org/10.1166/jbt.2023.3285","url":null,"abstract":"The pathogenesis and the mechanism of orally administered propranolol in the treatment of hemangioma are unclear. In this study, we evaluated the changes of xenograft hemangioma in nude mice after intervention with estradiol and propranolol. Raf-1 and p-ERK expression in xenograft hemangiomas\u0000 was assessed to evaluate their role in hemangioma proliferation and regression after treatment. A hemangioma xenograft model in nude mice was established. The successful xenograft specimens were selected and then randomized into control group, estradiol group and propranolol group. At the\u0000 date of injection, and on day 7 and 21 after injection, the morphological changes of xenograft hemangiomas were visually characterized and imaged by light microscopy. The distribution and expression Raf-1 and p-ERK protein was determined by immunohistochemical detection. In control group,\u0000 the xenografts increased gradually in volume, had a soft texture and their colors gradually turned red with observation of proliferation of endothelial cells and a capillary lumen that contained monolayer endothelial cells. In Estradiol group, the xenografts grew fast and increased significantly\u0000 in volume, had a soft texture and their colors were dark red with a hyperplasia of endothelial cells, irregular volume, and deranged and compact endothelial cells. More capillary lumens and sinuses were also seen. Raf-1 and p-ERK expression in estradiol group was significantly increased (P\u0000 < 0.05). In Propranolol group, the xenografts volume decreased, had a soft texture, and their colors turned gradually white with decreased number of proliferative endothelial cells. The vascular lumens, composed of endothelial cells, were larger, and some of them disappeared and were replaced\u0000 by fibrous connective tissue and vascular adipose tissue. Raf-1 and p-ERK expression in propranolol group was lower than estradiol and control group (P < 0.05). In conclusion, Raf-1/ERK signaling pathway may be involved in hemangioma. Estrogen and propranolol may regulate the proliferation\u0000 or regression of hemangioma through Raf-1/ERK signaling pathway.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42010580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Intravenous immunoglobulin (IVIG)-resistant Kawasaki disease (KD) is a complicated disorder, which can induce multiple-system damage. The pathogenic factor inducing KD remains unclear. The present study focused on identifying potential novel biomarkers for IVIG-resistant KD using integrated analyses. Eight IVIG-resistant KD samples and twelve IVIG-sensitive KD samples were included in the GSE18606 dataset. A Linear Model for Microarray Data (LIMMA) identified 504 differentially expressed genes (DEGs), An IVIG-resistant KD sample was compared with an IVIG-sensitive KD sample to identify 17 modules through weighted gene co-expression network analysis (WGCNA). A common gene (CG) is the intersection of DEGs and genes in the most significant module. Analysis of functional enrichment revealed that CGs were mainly enriched in TNF signaling pathways and NF-kappa B signaling pathways. Ten of these genes were selected as hub genes because of their high degree of connectivity (KLF1, AHSP, HBQ1, HBA2, HBA1, EPB42, GYPB, UBB, KRT1 and BPIFB2).
静脉免疫球蛋白(IVIG)抵抗性川崎病(KD)是一种复杂的疾病，可引起多系统损伤。诱发KD的致病因素尚不清楚。目前的研究重点是利用综合分析方法鉴定抗ivig KD的潜在新型生物标志物。GSE18606数据集中纳入了8个抗ivig的KD样本和12个对ivig敏感的KD样本。微阵列数据线性模型(Linear Model for Microarray Data, LIMMA)鉴定出504个差异表达基因(DEGs)，通过加权基因共表达网络分析(WGCNA)将抗ivig的KD样本与ivig敏感的KD样本进行比较，鉴定出17个模块。共同基因(CG)是deg和最重要模块中基因的交集。功能富集分析显示，CGs主要富集于TNF信号通路和nf - κ B信号通路。其中10个基因因其高度连通性而被选为枢纽基因(KLF1、AHSP、HBQ1、HBA2、HBA1、EPB42、GYPB、UBB、KRT1和BPIFB2)。
{"title":"Uncovering Potential Novel Biomarkers in Immunoglobulin-Resistant Kawasaki Disease Using Bioinformatics Analysis","authors":"Luoyi Hu","doi":"10.1166/jbt.2023.3278","DOIUrl":"https://doi.org/10.1166/jbt.2023.3278","url":null,"abstract":"Intravenous immunoglobulin (IVIG)-resistant Kawasaki disease (KD) is a complicated disorder, which can induce multiple-system damage. The pathogenic factor inducing KD remains unclear. The present study focused on identifying potential novel biomarkers for IVIG-resistant KD using integrated\u0000 analyses. Eight IVIG-resistant KD samples and twelve IVIG-sensitive KD samples were included in the GSE18606 dataset. A Linear Model for Microarray Data (LIMMA) identified 504 differentially expressed genes (DEGs), An IVIG-resistant KD sample was compared with an IVIG-sensitive KD sample to\u0000 identify 17 modules through weighted gene co-expression network analysis (WGCNA). A common gene (CG) is the intersection of DEGs and genes in the most significant module. Analysis of functional enrichment revealed that CGs were mainly enriched in TNF signaling pathways and NF-kappa B signaling\u0000 pathways. Ten of these genes were selected as hub genes because of their high degree of connectivity (KLF1, AHSP, HBQ1, HBA2, HBA1, EPB42, GYPB, UBB, KRT1 and BPIFB2).","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47271697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Sultan, M. Kamran, S. Almoammar, M. A. Al Jearah, Mariam Khan, Syed Junaid Mahmood, Ghulam Ahmed, Mirza Mahmood
Aim: To assess the effects of milk tea, carbonated drink, orange juice, and artificial saliva on the shear bond strength (SBS) of orthodontic brackets and assess their mode of failure under a scanning electron microscope (SEM). Material and Methods: Eighty non-carious extracted premolars were disinfected, etched, had primer applied and cured, and bracket bonded with light cure composite adhesive. These teeth were then immersed in separate media of milk tea, carbonated drink, orange juice, and artificial saliva for 10 minutes per day for three months. After which, they were thermocycled and subjected to shear stress in the Universal testing machine, and the shear load was recorded. Following debonding, the teeth were analyzed under SEM for failure analysis using adhesive remnant Index (ARI). Data were analyzed using ANOVA and Tukey multiple comparison tests. Results: Carbonated drink and milk tea showed comparable shear bond strength which differed significantly from that of orange juice and artificial saliva. Conclusion: Carbonated drink had the most erosive effect on the tooth’s surface and showed the least shear bond strength and adhesive remnant score than the teeth immersed in other media.
{"title":"pH Effects of Soda, Brew, and Citrus on Adhesive Bond Strength of Orthodontic Brackets to the Enamel. Scanning Electron Microscopy and Adhesive Remnant Index Analysis","authors":"H. Sultan, M. Kamran, S. Almoammar, M. A. Al Jearah, Mariam Khan, Syed Junaid Mahmood, Ghulam Ahmed, Mirza Mahmood","doi":"10.1166/jbt.2023.3287","DOIUrl":"https://doi.org/10.1166/jbt.2023.3287","url":null,"abstract":"Aim: To assess the effects of milk tea, carbonated drink, orange juice, and artificial saliva on the shear bond strength (SBS) of orthodontic brackets and assess their mode of failure under a scanning electron microscope (SEM). Material and Methods: Eighty non-carious\u0000 extracted premolars were disinfected, etched, had primer applied and cured, and bracket bonded with light cure composite adhesive. These teeth were then immersed in separate media of milk tea, carbonated drink, orange juice, and artificial saliva for 10 minutes per day for three months. After\u0000 which, they were thermocycled and subjected to shear stress in the Universal testing machine, and the shear load was recorded. Following debonding, the teeth were analyzed under SEM for failure analysis using adhesive remnant Index (ARI). Data were analyzed using ANOVA and Tukey multiple comparison\u0000 tests. Results: Carbonated drink and milk tea showed comparable shear bond strength which differed significantly from that of orange juice and artificial saliva. Conclusion: Carbonated drink had the most erosive effect on the tooth’s surface and showed the least shear bond\u0000 strength and adhesive remnant score than the teeth immersed in other media.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44532503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AMD, or age-related macular degeneration, is the fourth most common visual ailment leading to blindness worldwide and mostly affects persons over the age of 60. Early-stage blindness may be reduced with timely and precise screening. High-resolution analysis and identification of the retinal layers damaged by illness is made possible by optical coherence tomography (OCT), a diagnostic technique. Setting up a comprehensive eye screening system to identify AMD is a difficult task. Manually sifting through OCT pictures for anomalies is a time-consuming and error-prone operation. Automatic feature extraction from OCT images may speed up the diagnostic process and reduce the potential for human mistake. Historically, several methods have been developed to identify characteristics in OCT pictures. This thesis documents the development and evaluation of many such algorithms for the identification of AMD. In order to minimize the severity of AMD, retinal fundus images must be employed for early detection and classification. In this work, we develop a useful deep learning cloud-based AMD categorization model for wearables. The suggested model is DLCTO-AMDC model, a patient outfitted with a head-mounted camera (OphthoAI IoMT headset) may send retinaldehyde fundus imageries to a secure virtual server for analysis. The suggested AMD classification model employs Inception v3 as the feature extractor and a noise reduction approach based on midway point filtering (MPF). The deep belief network (DBN) model is also used to detect and classify AMD. Then, an AOA-inspired hyperparameter optimisation method is used to fine-tune the DBN parameters. To ensure the DLCTO-AMDC model would provide superior classification results, extensive simulations were done using the benchmark dataset. The findings prove the DLCTO-AMDC model is superior to other approaches already in use.
{"title":"An Efficient Investigation on Age-Related Macular Degeneration Using Deep Learning with Cloud-Based Teleophthalmology Architecture","authors":"P. Selvakumar, R. Arunprakash","doi":"10.1166/jbt.2023.3288","DOIUrl":"https://doi.org/10.1166/jbt.2023.3288","url":null,"abstract":"AMD, or age-related macular degeneration, is the fourth most common visual ailment leading to blindness worldwide and mostly affects persons over the age of 60. Early-stage blindness may be reduced with timely and precise screening. High-resolution analysis and identification of the\u0000 retinal layers damaged by illness is made possible by optical coherence tomography (OCT), a diagnostic technique. Setting up a comprehensive eye screening system to identify AMD is a difficult task. Manually sifting through OCT pictures for anomalies is a time-consuming and error-prone operation.\u0000 Automatic feature extraction from OCT images may speed up the diagnostic process and reduce the potential for human mistake. Historically, several methods have been developed to identify characteristics in OCT pictures. This thesis documents the development and evaluation of many such algorithms\u0000 for the identification of AMD. In order to minimize the severity of AMD, retinal fundus images must be employed for early detection and classification. In this work, we develop a useful deep learning cloud-based AMD categorization model for wearables. The suggested model is DLCTO-AMDC model,\u0000 a patient outfitted with a head-mounted camera (OphthoAI IoMT headset) may send retinaldehyde fundus imageries to a secure virtual server for analysis. The suggested AMD classification model employs Inception v3 as the feature extractor and a noise reduction approach based on midway point\u0000 filtering (MPF). The deep belief network (DBN) model is also used to detect and classify AMD. Then, an AOA-inspired hyperparameter optimisation method is used to fine-tune the DBN parameters. To ensure the DLCTO-AMDC model would provide superior classification results, extensive simulations\u0000 were done using the benchmark dataset. The findings prove the DLCTO-AMDC model is superior to other approaches already in use.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48585099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bao-Yin Zhang, Shuhao Wang, Linyuan Feng, J. Piao, Zhen-dong Lin, Yanqun Liu
Stomach adenocarcinoma (STAD) is a complex biological process involving multiple factors. Given the importance of the immune-related tumor microenvironment (TME) in STAD, investigating tumor-immune interactions and identifying novel prognostic and therapeutic targets in STAD is a promising avenue of research. S100A11 (S100 calcium-binding protein 11) is a class of proteins that transduces calcium-dependent cellular regulatory signals involved in cancer formation and development. Recent studies demonstrated that S100A gene families plays important roles in regulating immune cell infiltration of cancers. However, the exact role of S100A11 in STAD has yet to be fully understood. Therefore, we examined S100A11 expression in STAD and its normal tissues using GEPIA and the Human Protein Atlas, using the UALCAN database was used to analyze the relationship between S100A11 protein expression and clinical parameters, and using the GSCA database was used to analyze the correlation between S100A11 protein expression and various subtypes of STAD. We found that S100A11 mRNA levels were significantly upregulated in STAD tissues compared to normal tissues. Elevated S100A11 was significantly associated with poor overall survival (OS), first progression (FP) and post-progression survival (PPS) in multiple STAD patient populations.
{"title":"Expression of S100A11 Gene in Stomach Adenocarcinoma and Its Relationship with Immune Cell Infiltration and Prognosis","authors":"Bao-Yin Zhang, Shuhao Wang, Linyuan Feng, J. Piao, Zhen-dong Lin, Yanqun Liu","doi":"10.1166/jbt.2023.3286","DOIUrl":"https://doi.org/10.1166/jbt.2023.3286","url":null,"abstract":"Stomach adenocarcinoma (STAD) is a complex biological process involving multiple factors. Given the importance of the immune-related tumor microenvironment (TME) in STAD, investigating tumor-immune interactions and identifying novel prognostic and therapeutic targets in STAD is a promising\u0000 avenue of research. S100A11 (S100 calcium-binding protein 11) is a class of proteins that transduces calcium-dependent cellular regulatory signals involved in cancer formation and development. Recent studies demonstrated that S100A gene families plays important roles in regulating immune cell\u0000 infiltration of cancers. However, the exact role of S100A11 in STAD has yet to be fully understood. Therefore, we examined S100A11 expression in STAD and its normal tissues using GEPIA and the Human Protein Atlas, using the UALCAN database was used to analyze the relationship between S100A11\u0000 protein expression and clinical parameters, and using the GSCA database was used to analyze the correlation between S100A11 protein expression and various subtypes of STAD. We found that S100A11 mRNA levels were significantly upregulated in STAD tissues compared to normal tissues. Elevated\u0000 S100A11 was significantly associated with poor overall survival (OS), first progression (FP) and post-progression survival (PPS) in multiple STAD patient populations.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41343308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Alotaibi, Sarah Alnamlah, Rana M Almazroa, A. Alfouzan, S. Altaweel, H. Alshehri, N. Labban
We evaluated the effect of maintaining the applied torque for one minute on the reverse torque value (RTV) as compared to immediate torque application and retorquing after 10 minutes. A total of 48 screws were used to torque the abutment utilizing three different protocols (A–C); immediate torque application to the recommended 35 Ncm, torque applied to the recommended 35 Ncm, followed by retorquing of the same screw after 10 minutes and abutment screws were torqued to the recommended 35 Ncm, and the torque was held for one minute. The gap size between the implant and supra-structure was measured at three fixed points for each surface. Data were analyzed using SPSS software (α = 0.05). The mean RTV for 1 minute, immediate, and 10 minutes was 32.68±2.04 Ncm, 32.5±0.93 Ncm, and 30.54±0.90 Ncm, respectively. The difference between RTV in the 1-min and 10-min protocols was significant (P <0.05). Pearson’s coefficient demonstrated a negative correlation between RTV and the gap between the supra-structure and the implant interface (r =−0.43, P <0.01). When tightening abutment screws of implant-supported single crowns, holding the applied torque for 1 minute has proven to be efficient. Furthermore, larger gap values between the supra-structure and the implant inversely affect RTV, forcing the clinician to take caution to have a well-fitted supra-structure with as minimal a gap as possible.
{"title":"Effect of Different Applied Torque Maintenance Times on Detorque Values of Abutment Screws in Single Implant-Supported Fixed Prostheses","authors":"H. Alotaibi, Sarah Alnamlah, Rana M Almazroa, A. Alfouzan, S. Altaweel, H. Alshehri, N. Labban","doi":"10.1166/jbt.2023.3289","DOIUrl":"https://doi.org/10.1166/jbt.2023.3289","url":null,"abstract":"We evaluated the effect of maintaining the applied torque for one minute on the reverse torque value (RTV) as compared to immediate torque application and retorquing after 10 minutes. A total of 48 screws were used to torque the abutment utilizing three different protocols (A–C);\u0000 immediate torque application to the recommended 35 Ncm, torque applied to the recommended 35 Ncm, followed by retorquing of the same screw after 10 minutes and abutment screws were torqued to the recommended 35 Ncm, and the torque was held for one minute. The gap size between the implant and\u0000 supra-structure was measured at three fixed points for each surface. Data were analyzed using SPSS software (α = 0.05). The mean RTV for 1 minute, immediate, and 10 minutes was 32.68±2.04 Ncm, 32.5±0.93 Ncm, and 30.54±0.90 Ncm, respectively. The difference\u0000 between RTV in the 1-min and 10-min protocols was significant (P <0.05). Pearson’s coefficient demonstrated a negative correlation between RTV and the gap between the supra-structure and the implant interface (r =−0.43, P <0.01). When tightening abutment\u0000 screws of implant-supported single crowns, holding the applied torque for 1 minute has proven to be efficient. Furthermore, larger gap values between the supra-structure and the implant inversely affect RTV, forcing the clinician to take caution to have a well-fitted supra-structure with as\u0000 minimal a gap as possible.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46814303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The CD5L molecule (CD5L), also known as macrophage apoptosis inhibitor (AIM), has multiple functions in lipid metabolism and inflammatory processes. However, there is a lack of evaluation of CD5L in human tumors, especially its predictive role in HCC progression. The expression of CD5LmRNA in patients with hepatocellular Carcinoma was searched by The Tumor Genome Atlas (TCGA) database. CD5L had significant protein interactions with FASN, CD163, STAB2, and LILRB5, which were retrieved by the timer database. The relationship between CD5L survival and prognosis in HCC and hepatitis was analyzed by the KaplanMeier database. CD5L enrichment was analyzed by KEGG, Biological processes, Molecular functions, and Cellular components. CD5L expression was low in tumor tissues and high in neighboring tissues, showing a tumor inhibitory effect. Low expression of CD5L in patients with hepatitis is associated with poor prognosis. TP53 mutations with low CD5L expression accounted for a high proportion of HCC. The high expression of CD5L promotes the infiltration of CD4+ T cells, CD8+ T cells, macrophages, Tfh, and other cells, causing an immune response. We comprehensively evaluated the role of CD5L biomarkers in HCC, and CD5L may be a new target for tumor immunotherapy.
{"title":"CD5L is a Potential Biomarker for Clinical Prognosis and Immunotherapy of Hepatocellular Carcinoma","authors":"Bao-Yin Zhang, X. Ma, Zhen-dong Lin, Yanqun Liu","doi":"10.1166/jbt.2023.3283","DOIUrl":"https://doi.org/10.1166/jbt.2023.3283","url":null,"abstract":"The CD5L molecule (CD5L), also known as macrophage apoptosis inhibitor (AIM), has multiple functions in lipid metabolism and inflammatory processes. However, there is a lack of evaluation of CD5L in human tumors, especially its predictive role in HCC progression. The expression of CD5LmRNA\u0000 in patients with hepatocellular Carcinoma was searched by The Tumor Genome Atlas (TCGA) database. CD5L had significant protein interactions with FASN, CD163, STAB2, and LILRB5, which were retrieved by the timer database. The relationship between CD5L survival and prognosis in HCC and hepatitis\u0000 was analyzed by the KaplanMeier database. CD5L enrichment was analyzed by KEGG, Biological processes, Molecular functions, and Cellular components. CD5L expression was low in tumor tissues and high in neighboring tissues, showing a tumor inhibitory effect. Low expression of CD5L in patients\u0000 with hepatitis is associated with poor prognosis. TP53 mutations with low CD5L expression accounted for a high proportion of HCC. The high expression of CD5L promotes the infiltration of CD4+ T cells, CD8+ T cells, macrophages, Tfh, and other cells, causing an immune response. We comprehensively\u0000 evaluated the role of CD5L biomarkers in HCC, and CD5L may be a new target for tumor immunotherapy.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47145720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：