Maimaitiaili Niyazi, Jie Dai, Xin Eric Wang, Aikeremujiang Muheremu
Inflammatory factor stimulation secondary to lumbar disc herniation (LDH) is considered to be the main cause of lumbar back and lower extremity pain in patients with LDH. In the meanwhile, microRNAs have been reported to be effective in inhibiting the expression of several inflammatory factors. In the current study, we used rat LDH model to explore the feasibility of MSCs overexpressing microRNA-182-5p as a treatment option for LDH. Changes of inflammatory factors and changes of histological properties of dorsal root ganglion were observed to test the efficacy of this treatment option for lower back and extremity pain due secondary to LDH. All the rats survived by the end of eight week study period. The expression of TNF-α, IL-1, IL-6 both in dorsal root ganglia and blood serum were significantly lower in the experimental group than the control group (P <0.01). Histopathologic examination results showed better preserved tissue structural integrity of nerve ganglion in rats treated with BMSCs overexpressing microRNA-182-5p. Those results indicated that, BMSCs overexpressing microRNA-182-5p can significantly inhibit inflammatory reaction after LDH, and may be used as a therapeutic option to alleviate pain in patients with neurogenic pain after LDH.
{"title":"Overexpression of MicroRNA-182-5p Alleviates Pain in Rats with Lumbar Disc Herniation","authors":"Maimaitiaili Niyazi, Jie Dai, Xin Eric Wang, Aikeremujiang Muheremu","doi":"10.1166/jbt.2023.3300","DOIUrl":"https://doi.org/10.1166/jbt.2023.3300","url":null,"abstract":"Inflammatory factor stimulation secondary to lumbar disc herniation (LDH) is considered to be the main cause of lumbar back and lower extremity pain in patients with LDH. In the meanwhile, microRNAs have been reported to be effective in inhibiting the expression of several inflammatory\u0000 factors. In the current study, we used rat LDH model to explore the feasibility of MSCs overexpressing microRNA-182-5p as a treatment option for LDH. Changes of inflammatory factors and changes of histological properties of dorsal root ganglion were observed to test the efficacy of this treatment\u0000 option for lower back and extremity pain due secondary to LDH. All the rats survived by the end of eight week study period. The expression of TNF-α, IL-1, IL-6 both in dorsal root ganglia and blood serum were significantly lower in the experimental group than the control group\u0000 (P <0.01). Histopathologic examination results showed better preserved tissue structural integrity of nerve ganglion in rats treated with BMSCs overexpressing microRNA-182-5p. Those results indicated that, BMSCs overexpressing microRNA-182-5p can significantly inhibit inflammatory\u0000 reaction after LDH, and may be used as a therapeutic option to alleviate pain in patients with neurogenic pain after LDH.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46413230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hepatocellular carcinoma (HCC) is a major contributor to global cancer-related mortality, under-scoring the urgent need to identify effective prognostic markers. Single nucleotide polymorphisms (SNPs) play an increasingly important role in predicting the prognosis of patients with HCC. In this study, a cohort of 637 HCC patients was enrolled, and various online databases were comprehensively surveyed to identify SNPs that potentially impact HCC patient survival. The results of the Cox regression analysis demonstrated a significant association between rs3094165 and poor prognosis among patients with HCC. Further PSM analysis was also employed to control potential biases, and the results remained consistent. These findings suggest that SNPs can serve as reliable prognostic markers for HCC patients. Then, we conducted functional experiments to explore the molecular mechanism and confirmed that rs3094165 potentially regulates the progression of HCC by modulating the expression of the NDUFS1. Taken together, our study provides a comprehensive analysis of SNPs associated with HCC prognosis, and highlights the importance of rs3094165 as potential biomarkers for predicting poor prognosis of HCC patients. Our findings also shed light on the influence of rs3094165 on HCC progression by regulating NDUFS1 expression.
{"title":"The Mechanism Study of Single Nucleotide Polymorphism rs3094165 Regulating the Progression of Hepatocellular Carcinoma via Modulating NADH Dehydrogenase Ubiquinone Fe–S Protein-1","authors":"Ziyang Sun, Qingsong Cui, Yong Yu, Zhenhua Lin, Guang Jin, Hesong Cui, Enyue Yang","doi":"10.1166/jbt.2023.3294","DOIUrl":"https://doi.org/10.1166/jbt.2023.3294","url":null,"abstract":"Hepatocellular carcinoma (HCC) is a major contributor to global cancer-related mortality, under-scoring the urgent need to identify effective prognostic markers. Single nucleotide polymorphisms (SNPs) play an increasingly important role in predicting the prognosis of patients with HCC.\u0000 In this study, a cohort of 637 HCC patients was enrolled, and various online databases were comprehensively surveyed to identify SNPs that potentially impact HCC patient survival. The results of the Cox regression analysis demonstrated a significant association between rs3094165 and poor prognosis\u0000 among patients with HCC. Further PSM analysis was also employed to control potential biases, and the results remained consistent. These findings suggest that SNPs can serve as reliable prognostic markers for HCC patients. Then, we conducted functional experiments to explore the molecular mechanism\u0000 and confirmed that rs3094165 potentially regulates the progression of HCC by modulating the expression of the NDUFS1. Taken together, our study provides a comprehensive analysis of SNPs associated with HCC prognosis, and highlights the importance of rs3094165 as potential biomarkers for predicting\u0000 poor prognosis of HCC patients. Our findings also shed light on the influence of rs3094165 on HCC progression by regulating NDUFS1 expression.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48722735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives: This study aims to determine the variables influencing Saudi Arabia implantologists’ choice of the implant system. Materials and Methods: 78 dental implantologists in Saudi Arabia were interviewed and asked to complete a questionnaire. The questionnaire included age and gender demographic, followed by inquiries on the standards for choosing implant systems and used Likert scales. Results: Gender, place of employment, and specialization comparison between the doctor’s opinion and Fisher’s tests will be analyzed. The post-sale service (66.6%), size and prosthesis variety (59%), ease of surgical and prosthetic stages (56.4%), geometry and surface, and kind of connections were the most significant factors. The most relevant element in choosing an implant system was the implant system’s popularity (51.2%), and the least relevant aspect was a referral from a dentist (20.5%). One of the insignificant variables in choosing an implant system was the manufacturing country (25.6%). Patient preference (25.3%) is the irrelevant and negligible element influencing the choice of dental implant systems. Conclusions: This study indicated that most dentists prefer acid etching and blasting surface treatments.
{"title":"Factors Influencing Selection of Dental Implants Among Implantologists in Saudi Arabia","authors":"Ahmed A. Alzahrani, R. Kayal, Fatemah A. Tomishan","doi":"10.1166/jbt.2023.3295","DOIUrl":"https://doi.org/10.1166/jbt.2023.3295","url":null,"abstract":"Objectives: This study aims to determine the variables influencing Saudi Arabia implantologists’ choice of the implant system. Materials and Methods: 78 dental implantologists in Saudi Arabia were interviewed and asked to complete a questionnaire. The questionnaire\u0000 included age and gender demographic, followed by inquiries on the standards for choosing implant systems and used Likert scales. Results: Gender, place of employment, and specialization comparison between the doctor’s opinion and Fisher’s tests will be analyzed. The post-sale\u0000 service (66.6%), size and prosthesis variety (59%), ease of surgical and prosthetic stages (56.4%), geometry and surface, and kind of connections were the most significant factors. The most relevant element in choosing an implant system was the implant system’s popularity (51.2%), and\u0000 the least relevant aspect was a referral from a dentist (20.5%). One of the insignificant variables in choosing an implant system was the manufacturing country (25.6%). Patient preference (25.3%) is the irrelevant and negligible element influencing the choice of dental implant systems. Conclusions:\u0000 This study indicated that most dentists prefer acid etching and blasting surface treatments.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46907794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jamil M. A. S. Obaid, B. Alkadasi, M. Alahmari, Ahmed A. Alzahrani, T. A. AlDhelai, Shaima A. H. Mohammed, Ebtesam M. M. Al-Gora’ee, S. A. Elayah, Mohammed M. Al Moaleem
This study explored the secretion trait as a risk factor for periodontal disease progression. 162 patients suffering from periodontal disease attended the Outpatient Dental Clinics at the Faculty of Dentistry, Ibb University, and 69 healthy volunteers were enrolled in this study. Clinical evaluation for periodontal disease was carried out according to international guidelines and expressed by pocket probing depth (PPD), bleeding on probing (BOP), and clinical attachment level (CAL) parameters. A blood sample was collected and tested for blood grouping test, and a saliva sample was analyzed for secretion using hemagglutination inhibition test. Secretor patients were 82% compared with 64% of healthy control. Mean score of BOB and PPD and the clinical magnitude of CAL parameters were increased within secretors. The development of periodontal diseases increased among secretors by 2.61 times more than non-secretors (OR = 2.61, CI, 1.382–4.914, p = 0.004). Significant periodontal complaints associated with secretion are Bleeding gums (OR = 1.95, CI, 1.011–3.759, p = 0.049), malaligned teeth (OR = 4.49 (CI, 1.842–10.958), p = 0.0001), gingival recession (OR = 2.48, CI, 1.322–4.648, p = 0.007), and teeth decay (OR = 1.86, CI, 1.006–3.438, p = 0.05). Secretion of ABH antigens is risk factor for progression of periodontal diseases. The odds of periodontal complaints, bleeding gums, malaligned teeth, gingival recession, and tooth decay prove the ABH secretion effect.
{"title":"Periodontal Diseases and Salivary Secretion of ABH Blood Antigens in Yemeni Patients","authors":"Jamil M. A. S. Obaid, B. Alkadasi, M. Alahmari, Ahmed A. Alzahrani, T. A. AlDhelai, Shaima A. H. Mohammed, Ebtesam M. M. Al-Gora’ee, S. A. Elayah, Mohammed M. Al Moaleem","doi":"10.1166/jbt.2023.3307","DOIUrl":"https://doi.org/10.1166/jbt.2023.3307","url":null,"abstract":"This study explored the secretion trait as a risk factor for periodontal disease progression. 162 patients suffering from periodontal disease attended the Outpatient Dental Clinics at the Faculty of Dentistry, Ibb University, and 69 healthy volunteers were enrolled in this study. Clinical\u0000 evaluation for periodontal disease was carried out according to international guidelines and expressed by pocket probing depth (PPD), bleeding on probing (BOP), and clinical attachment level (CAL) parameters. A blood sample was collected and tested for blood grouping test, and a saliva sample\u0000 was analyzed for secretion using hemagglutination inhibition test. Secretor patients were 82% compared with 64% of healthy control. Mean score of BOB and PPD and the clinical magnitude of CAL parameters were increased within secretors. The development of periodontal diseases increased among\u0000 secretors by 2.61 times more than non-secretors (OR = 2.61, CI, 1.382–4.914, p = 0.004). Significant periodontal complaints associated with secretion are Bleeding gums (OR = 1.95, CI, 1.011–3.759, p = 0.049), malaligned teeth (OR = 4.49 (CI, 1.842–10.958), p\u0000 = 0.0001), gingival recession (OR = 2.48, CI, 1.322–4.648, p = 0.007), and teeth decay (OR = 1.86, CI, 1.006–3.438, p = 0.05). Secretion of ABH antigens is risk factor for progression of periodontal diseases. The odds of periodontal complaints, bleeding gums, malaligned\u0000 teeth, gingival recession, and tooth decay prove the ABH secretion effect.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49615141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Alsahhaf, E. Alhamdan, Fahim Vohra, T. Abduljabbar, Ziaullah Chaudhry, Isaac Kuyunov
Objective: To assess various inflammatory biomarkers CCL-20, BAF, RANK-L, IL-23, and osteoprotegerin from PICF along with clinical parameters in participants with peri-implant mucositis and peri-implantitis. Material and Methods: Participants were gathered i.e., 30 with periimplantitis, 32 with peri-implant mucositis, and 32 healthy. Peri-implant parameters PIPD, BoP, and PIPI were measured in all participants. PICF samples were collected to assess the level of biomarkers CCL-20; BAF; IL-23; RANK-L, and OPG. For periodontal parameters, ranges and means were measured. Kruskal Wallis test was used for comparison between groups. For categorical data sets, the Pearson Chi-Square test was applied. The Bonferroni test was used for multiple comparisons. Results: Peri-implant parameter BoP in peri-implant mucositis and peri-implantitis was significantly higher compared to controls (p < 0.05). PIPD with healthy peri-implant conditions was, significantly less compared to peri-implantitis, and peri-implant mucositis (p<0.05). PIPI demonstrated no significant difference throughout different peri-implant conditions. CCL-20 ng/mL in patients with peri-implant mucositis was found to be significantly higher (p < 0.05). BAF levels in peri-implantitis and peri-implant mucositis were comparable. The concentration of IL-23 ng/mL was found to be significantly lower in healthy controls (p < 0.05). Conclusion: Inflammatory biomarkers showed high levels of PICF in peri-implant disease patients compared to healthy controls. Individuals with peri-implant conditions experience the poor peri-implant probing depth and bleeding on probing.
{"title":"Novel Biomarker Evaluation in Peri-Implant Crevicular Fluid as Disease Indicators for Peri-Implant Health","authors":"A. Alsahhaf, E. Alhamdan, Fahim Vohra, T. Abduljabbar, Ziaullah Chaudhry, Isaac Kuyunov","doi":"10.1166/jbt.2023.3293","DOIUrl":"https://doi.org/10.1166/jbt.2023.3293","url":null,"abstract":"Objective: To assess various inflammatory biomarkers CCL-20, BAF, RANK-L, IL-23, and osteoprotegerin from PICF along with clinical parameters in participants with peri-implant mucositis and peri-implantitis. Material and Methods: Participants were gathered i.e., 30 with\u0000 periimplantitis, 32 with peri-implant mucositis, and 32 healthy. Peri-implant parameters PIPD, BoP, and PIPI were measured in all participants. PICF samples were collected to assess the level of biomarkers CCL-20; BAF; IL-23; RANK-L, and OPG. For periodontal parameters, ranges and means were\u0000 measured. Kruskal Wallis test was used for comparison between groups. For categorical data sets, the Pearson Chi-Square test was applied. The Bonferroni test was used for multiple comparisons. Results: Peri-implant parameter BoP in peri-implant mucositis and peri-implantitis was significantly\u0000 higher compared to controls (p < 0.05). PIPD with healthy peri-implant conditions was, significantly less compared to peri-implantitis, and peri-implant mucositis (p<0.05). PIPI demonstrated no significant difference throughout different peri-implant conditions. CCL-20\u0000 ng/mL in patients with peri-implant mucositis was found to be significantly higher (p < 0.05). BAF levels in peri-implantitis and peri-implant mucositis were comparable. The concentration of IL-23 ng/mL was found to be significantly lower in healthy controls (p < 0.05).\u0000 Conclusion: Inflammatory biomarkers showed high levels of PICF in peri-implant disease patients compared to healthy controls. Individuals with peri-implant conditions experience the poor peri-implant probing depth and bleeding on probing.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48104462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aims: Photon-induced Photoacoustic streaming (PIPS) as an adjunct to conventional irrigation (CI) using different final apical preparation diameters on smear layer (SL) Material and method: Clinical crown was removed from forty human mandibular molars standardizing root length to 18 mm. Chamber was opened and straight-line access was established Working length was kept 1 mm short of the apex. Teeth were randomly divided into 4 investigated groups based on the final apical diameter. Group-1 (F1+CI+PIPS), Group-2 (F2+CI+PIPS), Group-3 (F3+CI+PIPS) and Group-4 (F3+CI) (Control). CI protocol (2.5% NaOCl+17% EDTA+2.5% NaOCl+distilled water). SEM magnification of 5000x dentinal tubules’ patency at the apical third of root canals was assessed. ANOVA and Tukey’s HSD test were used for data analysis (p = 0.05) Results: Group-3 (F3+CI+PIPS displayed the highest mean scores (3.64±0.50) of SL removal from the apical third of the canal. However, Group-1 (F1+CI+PIPS) exhibited the lowest mean scores (1.27±0.47). Intergroup comparison analysis revealed that Group 1 and Group 2 (F2+CI+PIPS) presented the lowest and most comparable SL removal (p >0.05) Conclusion: Photon-induced Photoacoustic streaming should be considered as an adjunct therapy to improve the cleanliness of the Smear layer from the apical third.
{"title":"The Impact of Different Final Apical Preparation Diameters on the Efficiency of Photon-Induced Photoacoustic Streaming (PIPS) in Removing the Smear Layer at the Apical Third","authors":"K. Almadi, M. Alkahtany","doi":"10.1166/jbt.2023.3303","DOIUrl":"https://doi.org/10.1166/jbt.2023.3303","url":null,"abstract":"Aims: Photon-induced Photoacoustic streaming (PIPS) as an adjunct to conventional irrigation (CI) using different final apical preparation diameters on smear layer (SL) Material and method: Clinical crown was removed from forty human mandibular molars standardizing root\u0000 length to 18 mm. Chamber was opened and straight-line access was established Working length was kept 1 mm short of the apex. Teeth were randomly divided into 4 investigated groups based on the final apical diameter. Group-1 (F1+CI+PIPS), Group-2 (F2+CI+PIPS), Group-3 (F3+CI+PIPS) and Group-4\u0000 (F3+CI) (Control). CI protocol (2.5% NaOCl+17% EDTA+2.5% NaOCl+distilled water). SEM magnification of 5000x dentinal tubules’ patency at the apical third of root canals was assessed. ANOVA and Tukey’s HSD test were used for data analysis (p = 0.05) Results: Group-3\u0000 (F3+CI+PIPS displayed the highest mean scores (3.64±0.50) of SL removal from the apical third of the canal. However, Group-1 (F1+CI+PIPS) exhibited the lowest mean scores (1.27±0.47). Intergroup comparison analysis revealed that Group 1 and Group 2 (F2+CI+PIPS) presented the\u0000 lowest and most comparable SL removal (p >0.05) Conclusion: Photon-induced Photoacoustic streaming should be considered as an adjunct therapy to improve the cleanliness of the Smear layer from the apical third.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48494806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bone marrow-derived mesenchymal stem cells (BMSCs) affect EMT-related factors. miR-221-3p involves in several tumors. However, whether miR-221-3p affects cervical cancer (CC) cells co-cultured with BMSCs is unclear. BMSCs and CC cells were co-cultured, and transfected with miR-221-3p inhibitor followed by analysis of miR-221-3p level by real time PCR, cell proliferation, apoptosis activity, E-cadherin and Vimentin level, TGF-β1 secretion by ELISA as well as Smad1 and Smad2 expression. BMSCs upregulated miR-221-3p level in CC cells, increased cell proliferation and reduced apoptotic activity along with the decreased expression of EMT, increased TGF-β1 secretion and Smad1 and Smad2 expression (P <0.05). miR-221-3p inhibitor can reduce BMSCs’ effect on CC cells, and reverse the above changes (P <0.05). The co-culture of BMSCs promotes CC cell proliferation and invasion. Down-regulating miR-221-3p can change TGF-β1/Smad signaling pathway and affect malignant characteristics of CC cells.
骨髓间充质干细胞(BMSCs)影响emt相关因素。miR-221-3p参与多种肿瘤。然而，miR-221-3p是否影响与骨髓间充质干细胞共培养的宫颈癌(CC)细胞尚不清楚。将BMSCs与CC细胞共培养，转染miR-221-3p抑制剂，real - time PCR检测miR-221-3p水平、细胞增殖、凋亡活性、E-cadherin、Vimentin水平、ELISA检测TGF-β1分泌、Smad1、Smad2表达。BMSCs上调CC细胞中miR-221-3p水平，增加细胞增殖，降低细胞凋亡活性，同时降低EMT表达，增加TGF-β1分泌，增加Smad1、Smad2表达(P <0.05)。miR-221-3p抑制剂可降低BMSCs对CC细胞的作用，逆转上述变化(P <0.05)。骨髓间充质干细胞的共培养促进了CC细胞的增殖和侵袭。下调miR-221-3p可改变TGF-β1/Smad信号通路，影响CC细胞的恶性特征。
{"title":"miR-221-3p Modulates Cervical Cancer Cells Proliferation and Invasion After Co-Culture with Bone Marrow Mesenchymal Stem Cells (BMSCs)","authors":"Hongyan Cheng, Tao Jiang","doi":"10.1166/jbt.2023.3292","DOIUrl":"https://doi.org/10.1166/jbt.2023.3292","url":null,"abstract":"Bone marrow-derived mesenchymal stem cells (BMSCs) affect EMT-related factors. miR-221-3p involves in several tumors. However, whether miR-221-3p affects cervical cancer (CC) cells co-cultured with BMSCs is unclear. BMSCs and CC cells were co-cultured, and transfected with miR-221-3p\u0000 inhibitor followed by analysis of miR-221-3p level by real time PCR, cell proliferation, apoptosis activity, E-cadherin and Vimentin level, TGF-β1 secretion by ELISA as well as Smad1 and Smad2 expression. BMSCs upregulated miR-221-3p level in CC cells, increased cell proliferation\u0000 and reduced apoptotic activity along with the decreased expression of EMT, increased TGF-β1 secretion and Smad1 and Smad2 expression (P <0.05). miR-221-3p inhibitor can reduce BMSCs’ effect on CC cells, and reverse the above changes (P <0.05). The co-culture\u0000 of BMSCs promotes CC cell proliferation and invasion. Down-regulating miR-221-3p can change TGF-β1/Smad signaling pathway and affect malignant characteristics of CC cells.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43725903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Alqahtani, Hisham Abushaqqaf, Yazeed Assiry, Ahmed S Almslam, Fahad Al-obaid, Yazeed Alzamel, Ahmed Alhejazi, A. Alanzi
The aim was to compare the fluorescence intensity of tooth enamel, resin composite, lithium disilicate, and monolithic zirconia before and after thermocyclic aging. Lithium disilicate (LD) and monolithic Zirconia ceramic samples were fabricated with the CAD-CAM method. Resin composite specimens, of shades A1, A2, and A3, were prepared with photo-polymerization and putty mold. Extracted second molar teeth were obtained and sectioned to produce enamel specimens. Fluorescence assessment prior to thermocycling was performed using flurolog-Qm system before and after thermocycling. Samples were exposed to thermocycling (TC) for 30,000 cycles in distilled water at 5 °C and 55 °C for 30 s each, with 5 s between the baths. The means and standard deviations were compared using, t-test, analysis of variance, and Tukey–Kramer multiple comparisons test. The initial fluorescence intensities before thermocyclic ageing were highest in LD ceramic specimens (42579±817.1). The lowest fluorescence was observed in monolithic Zr specimen 14750±584.7. The reduction in fluorescence intensity after thermocyclic ageing was highest in LD specimens, however, the lowest difference was noted in composite A3 specimens. LD ceramic showed significantly high fluorescence among all materials before and after ageing. The fluorescence of the tested materials reduced with ageing and is expected to decrease intraorally with time.
{"title":"Comparison of Fluorescence of Tooth Enamel, Resin Composite, Lithium Disilicate and Monolithic Zirconia Under Thermocyclic Ageing","authors":"M. Alqahtani, Hisham Abushaqqaf, Yazeed Assiry, Ahmed S Almslam, Fahad Al-obaid, Yazeed Alzamel, Ahmed Alhejazi, A. Alanzi","doi":"10.1166/jbt.2023.3284","DOIUrl":"https://doi.org/10.1166/jbt.2023.3284","url":null,"abstract":"The aim was to compare the fluorescence intensity of tooth enamel, resin composite, lithium disilicate, and monolithic zirconia before and after thermocyclic aging. Lithium disilicate (LD) and monolithic Zirconia ceramic samples were fabricated with the CAD-CAM method. Resin composite\u0000 specimens, of shades A1, A2, and A3, were prepared with photo-polymerization and putty mold. Extracted second molar teeth were obtained and sectioned to produce enamel specimens. Fluorescence assessment prior to thermocycling was performed using flurolog-Qm system before and after thermocycling.\u0000 Samples were exposed to thermocycling (TC) for 30,000 cycles in distilled water at 5 °C and 55 °C for 30 s each, with 5 s between the baths. The means and standard deviations were compared using, t-test, analysis of variance, and Tukey–Kramer multiple comparisons test.\u0000 The initial fluorescence intensities before thermocyclic ageing were highest in LD ceramic specimens (42579±817.1). The lowest fluorescence was observed in monolithic Zr specimen 14750±584.7. The reduction in fluorescence intensity after thermocyclic ageing was highest in LD\u0000 specimens, however, the lowest difference was noted in composite A3 specimens. LD ceramic showed significantly high fluorescence among all materials before and after ageing. The fluorescence of the tested materials reduced with ageing and is expected to decrease intraorally with time.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48386717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pingquan Zhang, Sun Yijia, Zirong Huang, Liang Yujie, Weimin Zhu
Interleukin-1 (IL-1) is an important inflammatory factor in multi-organ inflammation and tissue damage. Interleukin-1 receptor antagonist (IL-1RA) is a bioactive receptor for IL-1. The interaction of these two—IL-1 and its particular receptor antagonist, IL-1RA—influences the propensity and severity of numerous illnesses. Importantly, therapies using IL-1RA have been applied in treatment of human inflammatory diseases like rheumatoid arthritis. In this study, we designed a “decoy” cell-derived nanocapsule, which uses stably-expressed HEK293T cells to display the IL-1RA on the outer surface of exosomes to act as a decoy antagonist against IL-1. After preparation, the decoy exosomes were characterized using Western Blot, transmission electron microscopy (TEM), and nanoparticle tracking analysis (NTA) to confirm whether they retained the correct size and shape of naturally-occurring exosomes. Results indicated that the IL-1Ra protein was successfully expressed on exosomes that had been secreted by HEK293T cells that were transfected with a pcDNA3.1(+)-IL-1RA-N-termSyntenin recombinant plasmid. This work provides a favorable, exosome-based tool for the targeted delivery of IL-1Ra for the treatment of joint inflammatory diseases.
{"title":"Decoy Interleukin-1 Receptor Antagonist on Extracellular Vesicles","authors":"Pingquan Zhang, Sun Yijia, Zirong Huang, Liang Yujie, Weimin Zhu","doi":"10.1166/jbt.2023.3280","DOIUrl":"https://doi.org/10.1166/jbt.2023.3280","url":null,"abstract":"Interleukin-1 (IL-1) is an important inflammatory factor in multi-organ inflammation and tissue damage. Interleukin-1 receptor antagonist (IL-1RA) is a bioactive receptor for IL-1. The interaction of these two—IL-1 and its particular receptor antagonist, IL-1RA—influences\u0000 the propensity and severity of numerous illnesses. Importantly, therapies using IL-1RA have been applied in treatment of human inflammatory diseases like rheumatoid arthritis. In this study, we designed a “decoy” cell-derived nanocapsule, which uses stably-expressed HEK293T cells\u0000 to display the IL-1RA on the outer surface of exosomes to act as a decoy antagonist against IL-1. After preparation, the decoy exosomes were characterized using Western Blot, transmission electron microscopy (TEM), and nanoparticle tracking analysis (NTA) to confirm whether they retained the\u0000 correct size and shape of naturally-occurring exosomes. Results indicated that the IL-1Ra protein was successfully expressed on exosomes that had been secreted by HEK293T cells that were transfected with a pcDNA3.1(+)-IL-1RA-N-termSyntenin recombinant plasmid. This work provides a favorable,\u0000 exosome-based tool for the targeted delivery of IL-1Ra for the treatment of joint inflammatory diseases.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42816523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hao Wang, Xuqing Mao, Li Ren, Cong-Cong Ma, Yibin Zhao, Lin Gan, Shanshan Zhang, Peng Gong, Yu-bo Lin, Xianming Lin
The presence of the Blood–Brain barrier (BBB) makes it difficult for therapeutic agents to reach the brain to treat brain diseases. As an emerging treatment, electroacupuncture (EA)-mediated BBB opening can temporarily increase the permeability of the brain blood vessels to increase the concentration of the therapeutic agent in the brain parenchyma, which offers great clinical benefits. Recent studies have suggested that BBB opening with excessive exposure levels may cause tissue/cell damage and short-term behavioral changes. Here, we investigated whether the EA-mediated BBB opening cause serious adverse events in the normal rat brain tissue and motor behavior, such as brain tissue damage, histopathologic alteration, or aggravated behavioral changes. EA was performed on the accupoint GV26 and GV20. Evans Blue Assay (EBA) and fluorescein isothiocyanate (FITC)–dextran Assay were performed to assess the BBB permeability. Immunofluorescence of GFAP-positive cells was marked and Hematoxylin-eosin (HE) Staining was carried out for assessing brain cortex damage. Tunel Assay was conducted to assess cell apoptosis. ELISA was used to measure the contents of TNF-α and IL-1β in brain tissue to explore the inflammatory response. The open field test, balance beam test and Y-Maze test were carried out to assess and neural behaviour. EA can induce an increase in BBB permeability on rats, allowing the macromolecular tracer Evans blue (EB) and Fluorescein Isothiocyanate (FITC)–Dextran to enter the brain. Histological analysis indicated that there was no obvious red blood cell leakage and a cellular apoptotic response in the brain tissue of rats with EA treatment. In addition, EA stimulation at specific parameters did not impair the rats’ motor ability, balance and coordination, and short-term spatial learning and memory. Our results suggest that EA can safely and effectively open the BBB in rats without causing brain damage and behavioral memory impairment.
血脑屏障(BBB)的存在使得治疗药物难以到达大脑治疗脑部疾病。电针(EA)介导的血脑屏障打开作为一种新兴的治疗方法，可以暂时增加脑血管的通透性，增加脑实质中治疗剂的浓度，具有很大的临床效益。最近的研究表明，过度暴露于血脑屏障可能导致组织/细胞损伤和短期行为改变。在这里，我们研究了ea介导的血脑屏障打开是否会在正常大鼠脑组织和运动行为中引起严重的不良事件，如脑组织损伤、组织病理学改变或加重的行为改变。在穴位GV26和GV20上进行EA。采用Evans Blue Assay (EBA)和异硫氰酸荧光素(FITC) -葡聚糖Assay评估血脑屏障的通透性。对gfap阳性细胞进行免疫荧光标记，并用苏木精-伊红(HE)染色评估脑皮质损伤。Tunel法检测细胞凋亡情况。ELISA法检测大鼠脑组织中TNF-α和IL-1β的含量，探讨炎症反应。采用裸地试验、平衡木试验和y型迷宫试验评估大鼠的神经行为。EA可以诱导大鼠血脑屏障通透性增加，使大分子示踪剂埃文斯蓝(EB)和异硫氰酸荧光素(FITC) -葡聚糖进入大脑。组织学分析表明，EA处理大鼠脑组织无明显的红细胞渗漏和细胞凋亡反应。此外，特定参数的电刺激对大鼠的运动能力、平衡协调能力和短期空间学习记忆能力没有损害。我们的研究结果表明，EA可以安全有效地打开大鼠血脑屏障，而不会造成脑损伤和行为记忆障碍。
{"title":"Safety Assessments of Electroacupuncture-Mediated Blood–Brain Barrier Opening: An Animal Study","authors":"Hao Wang, Xuqing Mao, Li Ren, Cong-Cong Ma, Yibin Zhao, Lin Gan, Shanshan Zhang, Peng Gong, Yu-bo Lin, Xianming Lin","doi":"10.1166/jbt.2023.3301","DOIUrl":"https://doi.org/10.1166/jbt.2023.3301","url":null,"abstract":"The presence of the Blood–Brain barrier (BBB) makes it difficult for therapeutic agents to reach the brain to treat brain diseases. As an emerging treatment, electroacupuncture (EA)-mediated BBB opening can temporarily increase the permeability of the brain blood vessels to increase\u0000 the concentration of the therapeutic agent in the brain parenchyma, which offers great clinical benefits. Recent studies have suggested that BBB opening with excessive exposure levels may cause tissue/cell damage and short-term behavioral changes. Here, we investigated whether the EA-mediated\u0000 BBB opening cause serious adverse events in the normal rat brain tissue and motor behavior, such as brain tissue damage, histopathologic alteration, or aggravated behavioral changes. EA was performed on the accupoint GV26 and GV20. Evans Blue Assay (EBA) and fluorescein isothiocyanate (FITC)–dextran\u0000 Assay were performed to assess the BBB permeability. Immunofluorescence of GFAP-positive cells was marked and Hematoxylin-eosin (HE) Staining was carried out for assessing brain cortex damage. Tunel Assay was conducted to assess cell apoptosis. ELISA was used to measure the contents of TNF-α\u0000 and IL-1β in brain tissue to explore the inflammatory response. The open field test, balance beam test and Y-Maze test were carried out to assess and neural behaviour. EA can induce an increase in BBB permeability on rats, allowing the macromolecular tracer Evans blue (EB) and\u0000 Fluorescein Isothiocyanate (FITC)–Dextran to enter the brain. Histological analysis indicated that there was no obvious red blood cell leakage and a cellular apoptotic response in the brain tissue of rats with EA treatment. In addition, EA stimulation at specific parameters did not impair\u0000 the rats’ motor ability, balance and coordination, and short-term spatial learning and memory. Our results suggest that EA can safely and effectively open the BBB in rats without causing brain damage and behavioral memory impairment.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47207326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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