A. AlShamrani, T. Almohareb, K. Al Ahdal, A. Maawadh, L. Al Deeb, A. Alrahlah
Aim: This study examines the SBS of resin-modified glass ionomer cement (RMGIC) on caries-affected dentin after using several cavity disinfectants (ICG activated by PDT, Pr, and SDF). Method: 40 CAD based on ICDAS criteria 3 and 4 teeth were chosen. Teeth were randomly divided into four groups (n = 10). Samples in Groups 1–4 were disinfected with chlorhexidine (CHX), indocyanine green (ICG), Propolis (Pr), and 38% silver diamine fluoride (SDF), respectively. Disinfected samples were bound to RMGIC and aged. SBS was evaluated using the universal testing machine. Stereomicroscope was used to assess bond failure. To compare many groups the Tukey’s Honestly Significant Difference (HSD) test was used. ANOVA was used to compare groups using mean values and standard deviations. Results: CHX-disinfected and RMGIC-bonded CAD had the highest bond values. Group 4 SDF-disinfected and RMGIC-bonded specimens had the lowest bond values. Group 1 samples disinfected with CHX and bonded with RMGIC had the highest bond integrity (p < 0.05) compared to other treatments. Conclusion: Chlorhexidine is the best disinfectant for caries-affected dentin before bonding with resin-modified glass ionomer cement.
{"title":"Artificial Ageing and Disinfection of Caries Affected Dentin on the Bond Integrity and Failure Analysis of Ion-Releasing Restorative Material","authors":"A. AlShamrani, T. Almohareb, K. Al Ahdal, A. Maawadh, L. Al Deeb, A. Alrahlah","doi":"10.1166/jbt.2023.3314","DOIUrl":"https://doi.org/10.1166/jbt.2023.3314","url":null,"abstract":"Aim: This study examines the SBS of resin-modified glass ionomer cement (RMGIC) on caries-affected dentin after using several cavity disinfectants (ICG activated by PDT, Pr, and SDF). Method: 40 CAD based on ICDAS criteria 3 and 4 teeth were chosen. Teeth were randomly divided into four groups (n = 10). Samples in Groups 1–4 were disinfected with chlorhexidine (CHX), indocyanine green (ICG), Propolis (Pr), and 38% silver diamine fluoride (SDF), respectively. Disinfected samples were bound to RMGIC and aged. SBS was evaluated using the universal testing machine. Stereomicroscope was used to assess bond failure. To compare many groups the Tukey’s Honestly Significant Difference (HSD) test was used. ANOVA was used to compare groups using mean values and standard deviations. Results: CHX-disinfected and RMGIC-bonded CAD had the highest bond values. Group 4 SDF-disinfected and RMGIC-bonded specimens had the lowest bond values. Group 1 samples disinfected with CHX and bonded with RMGIC had the highest bond integrity (p < 0.05) compared to other treatments. Conclusion: Chlorhexidine is the best disinfectant for caries-affected dentin before bonding with resin-modified glass ionomer cement.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"14 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Most studies have reported on the effects of bending rigidity using giant unilamellar liposomes. However, few studies have been conducted on nanosized liposomes. We studied the properties of DOPC liposomes of various sizes using atomic force microscopy (AFM). Two different topographies (convex and planar) of DOPC liposomes on a mica surface were observed in the tapping mode in the fluid. The topographic change of the liposomes could be attributed to the interaction force between the AFM tip and the liposome. Small DOPC liposomes (below 1 μm) showed a bending modulus (kbend) ranging between 10−18 and 10−20 J. The bending modulus was found to be size-dependent; it decreased as DOPC liposome size increased. In this study, images of unilamellar DOPC liposomes were obtained in the contact mode. The bending moduli of lipid vesicles from 100 to 1300 nm were measured using an AFM force curve. The bending modulus tended to increase as liposome size decreased. From experimental and theoretical perspectives, we showed that the value of the bending modulus is proportional to 1.5 power of the radius of the liposome.
{"title":"Size-Dependent Mechanical Property Measurement of 1,2-dioleoyl-SN-3-phosphocholine Liposome Using Atomic Force Microscopy","authors":"J. Kwak, J. Kwak, G. Kim","doi":"10.1166/jbt.2023.3315","DOIUrl":"https://doi.org/10.1166/jbt.2023.3315","url":null,"abstract":"Most studies have reported on the effects of bending rigidity using giant unilamellar liposomes. However, few studies have been conducted on nanosized liposomes. We studied the properties of DOPC liposomes of various sizes using atomic force microscopy (AFM). Two different topographies (convex and planar) of DOPC liposomes on a mica surface were observed in the tapping mode in the fluid. The topographic change of the liposomes could be attributed to the interaction force between the AFM tip and the liposome. Small DOPC liposomes (below 1 μm) showed a bending modulus (kbend) ranging between 10−18 and 10−20 J. The bending modulus was found to be size-dependent; it decreased as DOPC liposome size increased. In this study, images of unilamellar DOPC liposomes were obtained in the contact mode. The bending moduli of lipid vesicles from 100 to 1300 nm were measured using an AFM force curve. The bending modulus tended to increase as liposome size decreased. From experimental and theoretical perspectives, we showed that the value of the bending modulus is proportional to 1.5 power of the radius of the liposome.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"105 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Alsharidah, Mona Elsafadi, Osamah Al Rugaie, Amer Mahmood, Khalid M. Mohany, Khalid A. Al-Regaiey, K. Alyahya, Abdel-Moneim Hafez Abdel-Moneim, A. El Sadik, M. Abumaree
Aims: In this study, the authors aimed to examine the functional activities (adhesion, proliferation, and migration) of hMSCs under the effects of various concentrations of inflammatory mediators, such as TNFα or IFNg. Methods: HMSCs were exposed to various concentrations of the inflammatory mediators TNFα or IFNg, and the effects of TNFα or IFNg on the functional properties of hMSCs were determined using multiple functional assays (adhesion, proliferation, and migration). HMSC expression of genes (cytokine, chemokine, and adhesion molecule genes) was analyzed using real-time polymerase chain reaction (RT–PCR). Results: TNFα promoted their proliferation and migration especially at 100 ng/ml, while IFNg increased proliferation only at concentrations of 10 ng/ml and proliferation at 50 and 100 ng/ml. Conclusion: This study evaluated the suitability of hMSCs in treating inflammatory diseases, which are characterized by high levels of inflammatory mediators such as TNFα or IFNg, and proved that the treatment of hMSCs with TNFα or IFNg at higher concentrations has a protective effect.
{"title":"Therapeutic Potential of Human Mesenchymal Stem Cells Engineered with Different Concentrations of TNFα and IFNg","authors":"M. Alsharidah, Mona Elsafadi, Osamah Al Rugaie, Amer Mahmood, Khalid M. Mohany, Khalid A. Al-Regaiey, K. Alyahya, Abdel-Moneim Hafez Abdel-Moneim, A. El Sadik, M. Abumaree","doi":"10.1166/jbt.2023.3305","DOIUrl":"https://doi.org/10.1166/jbt.2023.3305","url":null,"abstract":"Aims: In this study, the authors aimed to examine the functional activities (adhesion, proliferation, and migration) of hMSCs under the effects of various concentrations of inflammatory mediators, such as TNFα or IFNg. Methods: HMSCs were exposed to various\u0000 concentrations of the inflammatory mediators TNFα or IFNg, and the effects of TNFα or IFNg on the functional properties of hMSCs were determined using multiple functional assays (adhesion, proliferation, and migration). HMSC expression of genes (cytokine, chemokine,\u0000 and adhesion molecule genes) was analyzed using real-time polymerase chain reaction (RT–PCR). Results: TNFα promoted their proliferation and migration especially at 100 ng/ml, while IFNg increased proliferation only at concentrations of 10 ng/ml and proliferation\u0000 at 50 and 100 ng/ml. Conclusion: This study evaluated the suitability of hMSCs in treating inflammatory diseases, which are characterized by high levels of inflammatory mediators such as TNFα or IFNg, and proved that the treatment of hMSCs with TNFα or IFNg\u0000 at higher concentrations has a protective effect.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49662080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yan Li, Lijie Sun, Lingli Zhou, Yonggang Ma, Mingyuan Liu
Separate stimulation by vitamin D3 and material surface chemistry has long witnessed and proved remarkable effects on biological behavior of osteoblasts, but combined stimulation effects of these two have not been reported yet. In this project, the surface of hydroxyl, amino and methyl groups were constructed by silicon-alkyl self-assembly technology. Then, chemical surfaces were to be integrated with vitamin D3 at different concentrations (10−7 mol/L, 10−8 mol/L, 10−9 mol/L) to stimulate MC3T3-E1 osteoblasts. It turned out to be quite successful in establishing the research platform for combined stimulation of material surface chemistry and vitamin D3. Meanwhile, indicators such as osteoblast adhesion spots, cytoskeleton formation, proliferation, and differentiation have been fully examined in the process for us to explore general rules and possible mechanism of combined stimulation effects of these two on early responses of osteoblasts. The results suggest that the combined effects of vitamin D3 and material surface chemistry can induce early responses of osteoblasts on different material surfaces, with differing response degrees. Combined stimulation of material surface chemistry-vitamin D3 causes adhesion plaque and cytoskeleton differences, leading to different early cell behaviors. These findings will provide a strong theoretical basis for the treatment of osteoporosis and the construction of tissue-engineered bone models in vitro.
{"title":"The Combined Effects of Material Surface Chemistry and Vitamin D3 on MC3T3-E1 Cells","authors":"Yan Li, Lijie Sun, Lingli Zhou, Yonggang Ma, Mingyuan Liu","doi":"10.1166/jbt.2023.3296","DOIUrl":"https://doi.org/10.1166/jbt.2023.3296","url":null,"abstract":"Separate stimulation by vitamin D3 and material surface chemistry has long witnessed and proved remarkable effects on biological behavior of osteoblasts, but combined stimulation effects of these two have not been reported yet. In this project, the surface of hydroxyl, amino\u0000 and methyl groups were constructed by silicon-alkyl self-assembly technology. Then, chemical surfaces were to be integrated with vitamin D3 at different concentrations (10−7 mol/L, 10−8 mol/L, 10−9 mol/L) to stimulate MC3T3-E1 osteoblasts.\u0000 It turned out to be quite successful in establishing the research platform for combined stimulation of material surface chemistry and vitamin D3. Meanwhile, indicators such as osteoblast adhesion spots, cytoskeleton formation, proliferation, and differentiation have been fully examined\u0000 in the process for us to explore general rules and possible mechanism of combined stimulation effects of these two on early responses of osteoblasts. The results suggest that the combined effects of vitamin D3 and material surface chemistry can induce early responses of osteoblasts\u0000 on different material surfaces, with differing response degrees. Combined stimulation of material surface chemistry-vitamin D3 causes adhesion plaque and cytoskeleton differences, leading to different early cell behaviors. These findings will provide a strong theoretical basis for the treatment\u0000 of osteoporosis and the construction of tissue-engineered bone models in vitro.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43995456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study examines the microhardness of five ceramic materials using CAD-CAM technology. Twenty-five disc-shaped specimens for the study, using I.P.S. Empress CAD (fully sintered), I.P.S. e.max CAD (30 minutes) and Vita Suprinity (26 minutes) milled in a pre-crystallized stage, Coprasmile (8 hours), and Zenostar milled in partially sintered stage (9 hours and 50 minutes). Each specimen had ten indentations, with a 1000-gram load applied for 10 seconds using a Vickers diamond indenter. Vickers values were automatically converted to microhardness using the formula Hv = 1.8544×F/d2. IBM SPSS 26 for statistical analysis, calculating microhardness values for each group. One-way ANOVA and Tukey HSD posthoc test compared differences between ceramic groups at a P <0.05 significance level. Results showed significant differences among the means (P <0.05), with Coprasmile, Zenostar, and Suprinity groups having the highest and lowest values, respectively.
{"title":"Surface Microhardness of Different Ceramic Materials Used for Esthetic Full-Ceramic Veneers","authors":"Esraa A. Attar, Ayman Aldharrab, Reem Ajaj","doi":"10.1166/jbt.2023.3298","DOIUrl":"https://doi.org/10.1166/jbt.2023.3298","url":null,"abstract":"The study examines the microhardness of five ceramic materials using CAD-CAM technology. Twenty-five disc-shaped specimens for the study, using I.P.S. Empress CAD (fully sintered), I.P.S. e.max CAD (30 minutes) and Vita Suprinity (26 minutes) milled in a pre-crystallized stage, Coprasmile\u0000 (8 hours), and Zenostar milled in partially sintered stage (9 hours and 50 minutes). Each specimen had ten indentations, with a 1000-gram load applied for 10 seconds using a Vickers diamond indenter. Vickers values were automatically converted to microhardness using the formula Hv = 1.8544×F/d2.\u0000 IBM SPSS 26 for statistical analysis, calculating microhardness values for each group. One-way ANOVA and Tukey HSD posthoc test compared differences between ceramic groups at a P <0.05 significance level. Results showed significant differences among the means (P <0.05),\u0000 with Coprasmile, Zenostar, and Suprinity groups having the highest and lowest values, respectively.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44175230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study aims to introduce an optimized method for the large-scale isolation of rat primary osteoblasts, suitable for exosome extraction. Methods: An advancement on the traditional secondary enzyme digestion method, manual bone grinding to clean connective tissue and ophthalmic cutting into fragments have been replaced with oscillating vortex rinsing and a 14 cm straight head shear in a centrifuge tube to cut bone fragments, significantly reducing the experimental time. The experiment was conducted on forty suckling rats. The experimental time of both the optimized and traditional methods were compared. The osteoblasts obtained through the optimized method were observed morphologically, identified by Alkaline phosphatase and alizarin red staining, and their proliferation activity was detected using the EdU method. Sufficient cell supernatant was collected to extract osteoblast-derived exosomes, identified by employing transmission electron microscopy, nanoparticle tracking analysis, and Western blot. Result: The total experimental time of the improved method (5.5±0.17 hours) was significantly shorter than the traditional method (7.25±0.23 hours) with a notable statistical difference (P <0.001); the osteoblasts obtained by the improved method displayed good growth status. On the seventh day, the cells matured and exhibited positive ALP staining. By the 21st day, mineralized nodules were formed, and alizarin red staining was positive. The EdU results showed that osteoblasts demonstrated healthy growth at both 24 and 48 hours, with a proliferation rate of (96.2%±1.3%) at 48 hours, slightly higher than that of (94.6%±2.8%) at 24 hours, with no significant statistical difference (P >0.05); Western blot showed positive results for CD9, CD63, and TSG101, and negative results for Calnexin. Conclusion: The optimized primary osteoblast isolation method can significantly improve experimental efficiency and is suitable for extracting osteoblast-derived exosomes.
{"title":"A Protocol for Large-Scale Isolation of Rat Primary Osteoblasts Suitable for Exosome Extraction","authors":"Yuhao Si, Shaoshuo Li, Yang Guo, Lining Wang, Yong Ma, Heng Yin","doi":"10.1166/jbt.2023.3299","DOIUrl":"https://doi.org/10.1166/jbt.2023.3299","url":null,"abstract":"Objective: This study aims to introduce an optimized method for the large-scale isolation of rat primary osteoblasts, suitable for exosome extraction. Methods: An advancement on the traditional secondary enzyme digestion method, manual bone grinding to clean connective\u0000 tissue and ophthalmic cutting into fragments have been replaced with oscillating vortex rinsing and a 14 cm straight head shear in a centrifuge tube to cut bone fragments, significantly reducing the experimental time. The experiment was conducted on forty suckling rats. The experimental time\u0000 of both the optimized and traditional methods were compared. The osteoblasts obtained through the optimized method were observed morphologically, identified by Alkaline phosphatase and alizarin red staining, and their proliferation activity was detected using the EdU method. Sufficient cell\u0000 supernatant was collected to extract osteoblast-derived exosomes, identified by employing transmission electron microscopy, nanoparticle tracking analysis, and Western blot. Result: The total experimental time of the improved method (5.5±0.17 hours) was significantly shorter\u0000 than the traditional method (7.25±0.23 hours) with a notable statistical difference (P <0.001); the osteoblasts obtained by the improved method displayed good growth status. On the seventh day, the cells matured and exhibited positive ALP staining. By the 21st day, mineralized\u0000 nodules were formed, and alizarin red staining was positive. The EdU results showed that osteoblasts demonstrated healthy growth at both 24 and 48 hours, with a proliferation rate of (96.2%±1.3%) at 48 hours, slightly higher than that of (94.6%±2.8%) at 24 hours, with no significant\u0000 statistical difference (P >0.05); Western blot showed positive results for CD9, CD63, and TSG101, and negative results for Calnexin. Conclusion: The optimized primary osteoblast isolation method can significantly improve experimental efficiency and is suitable for extracting\u0000 osteoblast-derived exosomes.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49097888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To explore the nursing intervention and its clinical effect on oral mucositis after methotrexate (MTX) chemotherapy in children with hematological tumors. Methods: Two-hundred children with hematological tumors admitted to Nanjing Children’s Hospital between January 2021 and August 2022 and treated with MTX chemotherapy after admission randomly dividing as control and experimental groups (n = 100 every group). Children given conventional clinical nursing and comprehensive oral care. Oral ulcer, quality of life, and satisfaction of children’s parents after intervention were compared between 2 groups. Results: SF-36 score after nursing intervention significantly increasing in experimental group compared with that of control group, with a statistically significant difference (p<0.05). Satisfaction of children’s parents significantly improving in experimental group compared with that of control group (p<0.05). Conclusion: Comprehensive oral nursing intervention is effective in preventing and treating oral mucositis, improving oral cleanliness and comfort, and developing good oral hygiene habits, which is conducive to clinical rehabilitation in children undergoing MTX chemotherapy for hematological tumors.
{"title":"Nursing Intervention in Oral Mucositis After Methotrexate Chemotherapy in Children with Hematological Tumors","authors":"Benying Yu, Lin Du, Wenyan Chen, Banghong Xu","doi":"10.1166/jbt.2023.3306","DOIUrl":"https://doi.org/10.1166/jbt.2023.3306","url":null,"abstract":"Objective: To explore the nursing intervention and its clinical effect on oral mucositis after methotrexate (MTX) chemotherapy in children with hematological tumors. Methods: Two-hundred children with hematological tumors admitted to Nanjing Children’s Hospital between\u0000 January 2021 and August 2022 and treated with MTX chemotherapy after admission randomly dividing as control and experimental groups (n = 100 every group). Children given conventional clinical nursing and comprehensive oral care. Oral ulcer, quality of life, and satisfaction of children’s\u0000 parents after intervention were compared between 2 groups. Results: SF-36 score after nursing intervention significantly increasing in experimental group compared with that of control group, with a statistically significant difference (p<0.05). Satisfaction of children’s\u0000 parents significantly improving in experimental group compared with that of control group (p<0.05). Conclusion: Comprehensive oral nursing intervention is effective in preventing and treating oral mucositis, improving oral cleanliness and comfort, and developing good oral\u0000 hygiene habits, which is conducive to clinical rehabilitation in children undergoing MTX chemotherapy for hematological tumors.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41482138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A growing number of related research papers have been published in recent months about COVID-19 as a global pandemic. Research directions and hot topics can be identified by bibliometric analysis of these publications. Using the Web of Science database, we scanned the global literature about COVID-19 from 2020 to 2022.A search using keywords such as “COVID-19” and “genetic polymorphisms” led to relevant publications. A bibliometric analysis of these articles was conducted using CiteSpace. We identified 438 publications on COVID-19 with genetic polymorphisms. In terms of citation counts, Nature ranked first with 271 citations. Veronica Tisato, Giuseppe Fiorentino, Naoki Yamamoto, and Ingrid Fricke-Galindo were the most productive authors (each having 4 articles). The most cited organization is Tehran University of Medical Sciences with fourteen articles. USA is the most prolific collaborator with 76 articles. The polymorphism in the interleukin-6 gene (IL-6) was first studied in 2020. ACE2 gene polymorphism has been the focus of research since 2021. A major contribution to this field was made by the USA. The relationship of ACE2 gene G8790A polymorphism with COVID-19 and severity may be future hotspots.
近几个月来，越来越多的相关研究论文将COVID-19视为全球大流行。通过对这些出版物的文献计量分析，可以确定研究方向和热点。利用Web of Science数据库，我们扫描了2020年至2022年有关COVID-19的全球文献。使用“COVID-19”和“遗传多态性”等关键词进行搜索，可以找到相关的出版物。使用CiteSpace对这些文章进行文献计量学分析。我们确定了438篇关于COVID-19基因多态性的出版物。引用次数方面，《自然》以271次排名第一。Veronica Tisato, Giuseppe Fiorentino, Naoki Yamamoto和Ingrid Fricke-Galindo是最多产的作者(每人有4篇文章)。被引用最多的组织是德黑兰医学科学大学，有14篇文章。美国是最多产的合作者，有76篇文章。白细胞介素-6基因(IL-6)多态性于2020年首次被研究。ACE2基因多态性自2021年以来一直是研究的重点。美国对这一领域作出了重大贡献。ACE2基因G8790A多态性与COVID-19及其严重程度的关系可能是未来研究的热点。
{"title":"Human Genetic Polymorphisms Contributing to Coronavirus Disease 2019 Susceptibility: A Bibliometric Analysis via CiteSpace","authors":"Wu Zuchuang, Pengfei Lv","doi":"10.1166/jbt.2023.3302","DOIUrl":"https://doi.org/10.1166/jbt.2023.3302","url":null,"abstract":"A growing number of related research papers have been published in recent months about COVID-19 as a global pandemic. Research directions and hot topics can be identified by bibliometric analysis of these publications. Using the Web of Science database, we scanned the global literature\u0000 about COVID-19 from 2020 to 2022.A search using keywords such as “COVID-19” and “genetic polymorphisms” led to relevant publications. A bibliometric analysis of these articles was conducted using CiteSpace. We identified 438 publications on COVID-19 with genetic polymorphisms.\u0000 In terms of citation counts, Nature ranked first with 271 citations. Veronica Tisato, Giuseppe Fiorentino, Naoki Yamamoto, and Ingrid Fricke-Galindo were the most productive authors (each having 4 articles). The most cited organization is Tehran University of Medical Sciences with fourteen\u0000 articles. USA is the most prolific collaborator with 76 articles. The polymorphism in the interleukin-6 gene (IL-6) was first studied in 2020. ACE2 gene polymorphism has been the focus of research since 2021. A major contribution to this field was made by the USA. The relationship of ACE2\u0000 gene G8790A polymorphism with COVID-19 and severity may be future hotspots.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47239823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The development of the intelligent expert system is required mandatorily today for the clinical analysis and to make the accurate diagnosis for disease treatment. Lung cancer diagnosis requires more thorough investigation than other disease processes since it impacts equally men and women with a higher fatality rate. Images from a computer tomography (CT) scan can give more useful information about a lung cancer’s diagnosis. Using CT scan input images, numerous machine learning as well as deep learning techniques are developed for the improvement of the medical treatment process. But when it comes to developing a precise and intelligent system, research still has a dark side. This research suggests a brand-new classification model that operates on the principles of optimal learning networks and capsules. Capsule network theory is used into the suggested framework to enhance classification maps and consequently lower the likelihood of overfitting issues. Additionally, Whale Optimized Feed Forward Layers (WO FFL) have been used in place of the traditional neural network in the suggested study to get the best classification of malignancies in lung CT scan. The suggested framework’s simulation results demonstrate improved F 1-score (99.98%), specificity (99.96%), sensitivity (99.95%), and accuracy (99.99%). Additionally, the suggested framework’s performance was compared to that of other traditional system, and several performance metrics indicated that the suggested paradigm outperformed the alternatives.
{"title":"LUNGCAPS-A Novel Hybrid Capsule Networks and Optimized Learning Framework for the Improved Classification of Lung Tumours","authors":"M. Manimegalai, P. Suresh Kumar","doi":"10.1166/jbt.2023.3297","DOIUrl":"https://doi.org/10.1166/jbt.2023.3297","url":null,"abstract":"The development of the intelligent expert system is required mandatorily today for the clinical analysis and to make the accurate diagnosis for disease treatment. Lung cancer diagnosis requires more thorough investigation than other disease processes since it impacts equally men and\u0000 women with a higher fatality rate. Images from a computer tomography (CT) scan can give more useful information about a lung cancer’s diagnosis. Using CT scan input images, numerous machine learning as well as deep learning techniques are developed for the improvement of the medical\u0000 treatment process. But when it comes to developing a precise and intelligent system, research still has a dark side. This research suggests a brand-new classification model that operates on the principles of optimal learning networks and capsules. Capsule network theory is used into the suggested\u0000 framework to enhance classification maps and consequently lower the likelihood of overfitting issues. Additionally, Whale Optimized Feed Forward Layers (WO FFL) have been used in place of the traditional neural network in the suggested study to get the best classification of malignancies in\u0000 lung CT scan. The suggested framework’s simulation results demonstrate improved F 1-score (99.98%), specificity (99.96%), sensitivity (99.95%), and accuracy (99.99%). Additionally, the suggested framework’s performance was compared to that of other traditional system, and\u0000 several performance metrics indicated that the suggested paradigm outperformed the alternatives.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42534496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fangchen Liu, Rui Ma, Yidan Ma, Geng Li, Yongsheng Lin, Bin Ma, Lin Xu, Jianye Zhou
Bone scaffolds are a kind of bone repair material, but bone scaffold infection is a common and serious clinical problem that has not been well studied in the context of the oral microbiota. We prepared two different bone scaffold materials with different pore sizes from adult and fetal bovine cancellous bones and compared them with hydroxyapatite (HA) powder. After incubation, we facilitated the initial colonization by the oral microbiota on the three materials by using the saliva of healthy people. Through high-throughput sequencing and analysis, we found that the different pore sizes of the bone scaffold materials had a certain influence on the initial colonization by the oral microbiota, affecting β-diversity and functional profiles. Redundancy analysis (RDA) and cooccurrence analysis indicated that initial microbial colonization by the oral microbiota in fetal bone scaffold materials with smaller pore sizes was more likely to cause infection. In addition, there may be some functional bacteria that play an interactive role in early colonization by the oral microflora in bone scaffold materials, such as those of the genera Bacillus and Lactobacillus.
{"title":"The Influence of Different Pore Sizes of Bovine Bone Scaffold Material on the Oral Microbiota","authors":"Fangchen Liu, Rui Ma, Yidan Ma, Geng Li, Yongsheng Lin, Bin Ma, Lin Xu, Jianye Zhou","doi":"10.1166/jbt.2023.3304","DOIUrl":"https://doi.org/10.1166/jbt.2023.3304","url":null,"abstract":"Bone scaffolds are a kind of bone repair material, but bone scaffold infection is a common and serious clinical problem that has not been well studied in the context of the oral microbiota. We prepared two different bone scaffold materials with different pore sizes from adult and fetal\u0000 bovine cancellous bones and compared them with hydroxyapatite (HA) powder. After incubation, we facilitated the initial colonization by the oral microbiota on the three materials by using the saliva of healthy people. Through high-throughput sequencing and analysis, we found that the different\u0000 pore sizes of the bone scaffold materials had a certain influence on the initial colonization by the oral microbiota, affecting β-diversity and functional profiles. Redundancy analysis (RDA) and cooccurrence analysis indicated that initial microbial colonization by the oral microbiota\u0000 in fetal bone scaffold materials with smaller pore sizes was more likely to cause infection. In addition, there may be some functional bacteria that play an interactive role in early colonization by the oral microflora in bone scaffold materials, such as those of the genera Bacillus\u0000 and Lactobacillus.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42615591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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