Xiao-Jiao Nie, Huiru Cao, Wumidan Abudureheman, Chen Lin
The recurrence and prognosis of advanced cervical cancer patients is an unresolved medical need. To improve prognosis and bring new strategies to more curable stages of the disease, such as high-risk locally advanced disease patients and low metastatic or small volume disease patients. After culturing cervical cancer cells in vitro, they were treated with different concentrations of cisplatin and carboplatin drugs for 24, 48, and 72 hours respectively. Detected the inhibitory rate of different treatment groups on cervical cancer cells using CCK-8 detection, To observe live and dead cells through staining experiments. The results showed that different concentrations of cisplatin and carboplatin have significant inhibitory effects on cervical cancer cells. However, the inhibitory effect of cisplatin and carboplatin in the high concentration group on cervical cancer cells were significantly greater than that in the low concentration treatment group. The sensitivity of cervical cancer cells to cisplatin was similar with carboplatin, and the sensitivity to cisplatin was better than that of carboplatin. We believe that targeted therapy can be combined with chemotherapy drugs to enhance the anti-tumor effect of cisplatin. When the toxic side effects of cisplatin cannot be overcome, carboplatin can be considered to replace cisplatin in the treatment of cancer.
{"title":"Inhibitory Effect of Metal Complexes Cisplatin and Carboplatin of Cervical Cancer Cells","authors":"Xiao-Jiao Nie, Huiru Cao, Wumidan Abudureheman, Chen Lin","doi":"10.1166/jbt.2023.3290","DOIUrl":"https://doi.org/10.1166/jbt.2023.3290","url":null,"abstract":"The recurrence and prognosis of advanced cervical cancer patients is an unresolved medical need. To improve prognosis and bring new strategies to more curable stages of the disease, such as high-risk locally advanced disease patients and low metastatic or small volume disease patients.\u0000 After culturing cervical cancer cells in vitro, they were treated with different concentrations of cisplatin and carboplatin drugs for 24, 48, and 72 hours respectively. Detected the inhibitory rate of different treatment groups on cervical cancer cells using CCK-8 detection, To observe\u0000 live and dead cells through staining experiments. The results showed that different concentrations of cisplatin and carboplatin have significant inhibitory effects on cervical cancer cells. However, the inhibitory effect of cisplatin and carboplatin in the high concentration group on cervical\u0000 cancer cells were significantly greater than that in the low concentration treatment group. The sensitivity of cervical cancer cells to cisplatin was similar with carboplatin, and the sensitivity to cisplatin was better than that of carboplatin. We believe that targeted therapy can be combined\u0000 with chemotherapy drugs to enhance the anti-tumor effect of cisplatin. When the toxic side effects of cisplatin cannot be overcome, carboplatin can be considered to replace cisplatin in the treatment of cancer.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47405065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yan Li, Lijie Sun, Lingli Zhou, Yonggang Ma, Mingyuan Liu
In recent years, polyvinyl alcohol hydrogel has been repeatedly confronted with urgent needs for more enhanced bio-activity in the field of bone tissue engineering. We used sulfydryl-alkyne click chemical reaction to prepare a bifunctional polyvinyl alcohol hydrogel containing RGD peptide and hyaluronic acid. Polyvinyl alcohol grafted with hyaluronic acid can reduce degradation rate and improve mechanical properties. RGD peptide can improve cell adhesion and migration on material surface. The bifunctional polyvinyl alcohol hydrogel is prepared by click chemistry reaction and characterized by scanning electron microscopy, 1H NMR, Ellman’s test and Kjeldahl nitrogen determination. The physical properties of bifunctional polyvinyl alcohol hydrogel are evaluated in terms of tensile properties and water contact angle test. According to varying hydrogel composition, five groups are designed to conduct the experiment as follows: Control group, PVA group, PVA-HA group, PVAPA-RGD group, and PVA-HA-SH/PVAPA-RGD group, each of which is to be co-cultured with MC3T3-E1 cells to observe cell morphology by laser scanning confocal microscope. Then, CCK-8 method and cell counting method were used to detect the proliferation activity and cell adhesion rate, respectively. ALP activity and qRT-PCR were used to detect osteogenic differentiation and expression of osteogenic marker genes ALP, OCN, Runx2, Col I and OPN. The experimental results show that the tensile strength of the bifunctional polyvinyl alcohol hydrogel is 1.42±0.61 MPa, with tensile modulus as 0.58±0.03 MPa, and elongation at break as 297.53±42.36%. The water contact angle is 18.40±2.2°. After three days of culture, the proliferation activity ranks in order as PVA-HA-SH/PVAPA-RGD group > PVA-RGD group > PVA-HA group > PVA group > Control group. After 4 hours of culture, cell adhesion is sequenced as PVA-HA-SH/PVAPA-RGD group > bicomponent group > PVA group > Control group. After 48 hours of culture, immunofluorescence staining demonstrates more sufficient cellular spreading and adherence, clearer actin fibers, more vinculin numbers in PVA-HA-SH/PVAPA-RGD group. In addition, significantly higher ALP activity (after 3 days’ culturing) and osteogenic gene expression level (after 7 days’ culturing) have been witnessed in PVA-HA-SH/PVAPA-RGD group than those of other groups. The overall experimental results have confirmed its excellent hydrophilicity and fine mechanical properties of the bifunctional polyvinyl alcohol hydrogel system, which would exert a remarkable effect on proliferation, adhesion and osteogenic differentiation of MC3T3-E1 cells.
{"title":"Preparation, Characterization and Osteoblastic Compatibility Evaluation of Bifunctional Polyvinyl Alcohol Hydrogels Containing RGD and Hyaluronic Acid","authors":"Yan Li, Lijie Sun, Lingli Zhou, Yonggang Ma, Mingyuan Liu","doi":"10.1166/jbt.2023.3279","DOIUrl":"https://doi.org/10.1166/jbt.2023.3279","url":null,"abstract":"In recent years, polyvinyl alcohol hydrogel has been repeatedly confronted with urgent needs for more enhanced bio-activity in the field of bone tissue engineering. We used sulfydryl-alkyne click chemical reaction to prepare a bifunctional polyvinyl alcohol hydrogel containing RGD peptide\u0000 and hyaluronic acid. Polyvinyl alcohol grafted with hyaluronic acid can reduce degradation rate and improve mechanical properties. RGD peptide can improve cell adhesion and migration on material surface. The bifunctional polyvinyl alcohol hydrogel is prepared by click chemistry reaction and\u0000 characterized by scanning electron microscopy, 1H NMR, Ellman’s test and Kjeldahl nitrogen determination. The physical properties of bifunctional polyvinyl alcohol hydrogel are evaluated in terms of tensile properties and water contact angle test. According to varying hydrogel\u0000 composition, five groups are designed to conduct the experiment as follows: Control group, PVA group, PVA-HA group, PVAPA-RGD group, and PVA-HA-SH/PVAPA-RGD group, each of which is to be co-cultured with MC3T3-E1 cells to observe cell morphology by laser scanning confocal microscope. Then,\u0000 CCK-8 method and cell counting method were used to detect the proliferation activity and cell adhesion rate, respectively. ALP activity and qRT-PCR were used to detect osteogenic differentiation and expression of osteogenic marker genes ALP, OCN, Runx2, Col I and OPN. The experimental results\u0000 show that the tensile strength of the bifunctional polyvinyl alcohol hydrogel is 1.42±0.61 MPa, with tensile modulus as 0.58±0.03 MPa, and elongation at break as 297.53±42.36%. The water contact angle is 18.40±2.2°. After three days of culture, the proliferation\u0000 activity ranks in order as PVA-HA-SH/PVAPA-RGD group > PVA-RGD group > PVA-HA group > PVA group > Control group. After 4 hours of culture, cell adhesion is sequenced as PVA-HA-SH/PVAPA-RGD group > bicomponent group > PVA group > Control group. After 48 hours of culture,\u0000 immunofluorescence staining demonstrates more sufficient cellular spreading and adherence, clearer actin fibers, more vinculin numbers in PVA-HA-SH/PVAPA-RGD group. In addition, significantly higher ALP activity (after 3 days’ culturing) and osteogenic gene expression level (after 7\u0000 days’ culturing) have been witnessed in PVA-HA-SH/PVAPA-RGD group than those of other groups. The overall experimental results have confirmed its excellent hydrophilicity and fine mechanical properties of the bifunctional polyvinyl alcohol hydrogel system, which would exert a remarkable\u0000 effect on proliferation, adhesion and osteogenic differentiation of MC3T3-E1 cells.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44329171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dongtao Wang, Yu Yang, Tianyong Zhao, Fang Wang, Jingrui Qi, Lu Sui, L. Xing
Background: Adenoid cystic carcinoma (ACC) is a common tumor of the head and neck. It is characterized by slow growth, strong invasiveness, easy recurrence and distant organ metastasis. Bcl-2 and NF-κB are indicated to relate to tumor growth, proliferation and apoptosis. This study aims to analyze the relationship between Bcl-2, NF-κB and ACC. Methods: With ELISA method to detect Bcl-2 and NF-κB expression in the blood of patients with ACC, patients with benign adenoma and healthy controls, and the expression of Bcl-2 and NF-κB in tissues was detected by immunohistochemistry, and their relationship with ACC was analyzed. relevance. Relationships between clinicopathological features. Results: The levels of Bcl-2 (4.16±1.34 ng/ml) and NF-κB (4.47 ± 1.37 ng/ml) as well as positive rates of Bcl-2 and NF-κB (87.5% and 85%) were significantly elevated in the ACC group (P <0.05). Bcl-2 was positively correlated with NF-κB (P <0.05). Of note, Bcl-2 and NF-κB expressions were correlated with the incidence frequency, histological type, clinical stage, lymph node metastasis and distant metastasis of ACC patients (P < 0.05) as the distant metastasis is an independent risk factor. Conclusion: In summary, Bcl-2 and NF-κB expression in ACC patients is associated with the clinicopathological features of ACC.
{"title":"Expression of Lymphoma-2 Gene and NF-κB in Human Adenoid Cystic Carcinoma Tissue and Its Relation with Disease Prognosis","authors":"Dongtao Wang, Yu Yang, Tianyong Zhao, Fang Wang, Jingrui Qi, Lu Sui, L. Xing","doi":"10.1166/jbt.2023.3267","DOIUrl":"https://doi.org/10.1166/jbt.2023.3267","url":null,"abstract":"Background: Adenoid cystic carcinoma (ACC) is a common tumor of the head and neck. It is characterized by slow growth, strong invasiveness, easy recurrence and distant organ metastasis. Bcl-2 and NF-κB are indicated to relate to tumor growth, proliferation and apoptosis.\u0000 This study aims to analyze the relationship between Bcl-2, NF-κB and ACC. Methods: With ELISA method to detect Bcl-2 and NF-κB expression in the blood of patients with ACC, patients with benign adenoma and healthy controls, and the expression of Bcl-2 and NF-κB\u0000 in tissues was detected by immunohistochemistry, and their relationship with ACC was analyzed. relevance. Relationships between clinicopathological features. Results: The levels of Bcl-2 (4.16±1.34 ng/ml) and NF-κB (4.47 ± 1.37 ng/ml) as well as positive rates\u0000 of Bcl-2 and NF-κB (87.5% and 85%) were significantly elevated in the ACC group (P <0.05). Bcl-2 was positively correlated with NF-κB (P <0.05). Of note, Bcl-2 and NF-κB expressions were correlated with the incidence frequency, histological\u0000 type, clinical stage, lymph node metastasis and distant metastasis of ACC patients (P < 0.05) as the distant metastasis is an independent risk factor. Conclusion: In summary, Bcl-2 and NF-κB expression in ACC patients is associated with the clinicopathological\u0000 features of ACC.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46177394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Alqahtani, A. AlHelal, A. Alayad, A. Alotaibi, Fahad A. Alfarraj, Omar S. Alosaimi, Ziyad H. Alharbi
Aim: To examine the cutting efficiency and amount of material removed from crown-cutting diamond burs from a different manufacturer on zirconia using an electric handpiece. Material and Methods: The performance of super coarse diamond bur round-end from five different manufacturers that were commercially advertised as zirconium crown cutters were assessed. The five manufacturers and their respective diamond bur models tested were super coarse Group 1: Dimond bur, Group 2: Zirconia Diamond Bur, Round End Cylinder, Meisinger, Group 3: 4ZR Zirconia Cutter Diamond Crown Removal Bur, Group 4: Brasseler Dental Diamond Bur, Group 5: Expanded SS White® Lineup of Zirconia Cutting Diamonds. The qualitative assessment of diamond burs (before zirconia cutting) and (after zirconia cutting) was assessed using a scanning electron microscope. The mean cutting distance and material removed for each group were calculated and analyzed using one-way ANOVA. Results: The highest cutting efficiency was found in group 2 and the lowest efficiency was observed in group 5. Post-SEM images of rotary zirconia cutting instruments in groups (Post G1-G5) demonstrate pullout, wear, chipping, and loss of the diamond grains in general. Conclusion: Zirconia Diamond Bur (G2) was found to be suitable. Whereas, (G5) Expanded SS White® Lineup of Zirconia Cutting Great White®Z Diamonds is less effective.
{"title":"Assessment of Diamond Rotary Instruments Advertised for Cutting 3Y-TZP Restorations. A Scanning Electron Microscope Analysis","authors":"A. Alqahtani, A. AlHelal, A. Alayad, A. Alotaibi, Fahad A. Alfarraj, Omar S. Alosaimi, Ziyad H. Alharbi","doi":"10.1166/jbt.2023.3274","DOIUrl":"https://doi.org/10.1166/jbt.2023.3274","url":null,"abstract":"Aim: To examine the cutting efficiency and amount of material removed from crown-cutting diamond burs from a different manufacturer on zirconia using an electric handpiece. Material and Methods: The performance of super coarse diamond bur round-end from five different\u0000 manufacturers that were commercially advertised as zirconium crown cutters were assessed. The five manufacturers and their respective diamond bur models tested were super coarse Group 1: Dimond bur, Group 2: Zirconia Diamond Bur, Round End Cylinder, Meisinger, Group 3: 4ZR Zirconia Cutter\u0000 Diamond Crown Removal Bur, Group 4: Brasseler Dental Diamond Bur, Group 5: Expanded SS White® Lineup of Zirconia Cutting Diamonds. The qualitative assessment of diamond burs (before zirconia cutting) and (after zirconia cutting) was assessed using a scanning electron microscope.\u0000 The mean cutting distance and material removed for each group were calculated and analyzed using one-way ANOVA. Results: The highest cutting efficiency was found in group 2 and the lowest efficiency was observed in group 5. Post-SEM images of rotary zirconia cutting instruments in groups\u0000 (Post G1-G5) demonstrate pullout, wear, chipping, and loss of the diamond grains in general. Conclusion: Zirconia Diamond Bur (G2) was found to be suitable. Whereas, (G5) Expanded SS White® Lineup of Zirconia Cutting Great White®Z Diamonds is less effective.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44605023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The stomach and all of its appendages, which include the oesophagus, duodenum, small intestine, and large intestine, amongst others, all play a crucial function within this system. Stomach dysrhythmias, which are linked to problems with the movement of gastrointestinal contents, affect a significant number of individuals all over the globe. These problems include inappropriate digestion (dyspepsia), nausea (vomiting sensation) for no apparent reason, vomiting, abdominal pain, stomach ulcers, gastroesophageal reflux disease, and other disorders. During the process of finding the anomalies, it is possible that a number of techniques, including as imaging, endoscopy, electrogastrogram, and clinical analysis, will be used. Electrogastrography signals, also known as electrogastrograms (EGG), were captured using surface Ag/AgCl electrodes that were put over the stomach in 20 healthy persons before the data was gathered and pre-processed. The datasets were produced from these signals (8 Females and 12 Males). In addition to this, the datasets were obtained from 10 individuals who were suffering from various stomach illnesses (3 Females and 8 Males). In the stage known as “pre-processing,” which needs the obtained dataset to be treated in advance, any noise that was present in the signal is removed. In order to rid the data of any noise and increase the overall quality of the input data, a technique that is known as the Wiener filter is used. A technique known as Hybrid Grey Wolf Optimization with Particle Swarm Optimization is utilized in the process of feature selection. This algorithm is responsible for removing any extraneous data from the features that have been collected from the signal. The procedure is sped up as a result of this. The classifiers get the qualities that have been chosen as their input in order to carry out an analysis of the many stomach disorders, such as primary gastric lymphoma, gastrointestinal stromal tumour (GIST), and neuroendocrine tumor. This enables the classifiers to do the analysis (carcinoid). The Multi-class Feed Forward Neural Network Classifier (MCFFN) is used to carry out the classification process. This classifier provides the stages along with the classes. The accuracy, sensitivity, and specificity of the classification process are taken into account in the calculation of performance measures.
{"title":"Gastric Disorder Analysis Using Hybrid Optimization with Machine Learning","authors":"G. Gurumoorthy, S. Ganesh Vaidyanathan","doi":"10.1166/jbt.2023.3269","DOIUrl":"https://doi.org/10.1166/jbt.2023.3269","url":null,"abstract":"The stomach and all of its appendages, which include the oesophagus, duodenum, small intestine, and large intestine, amongst others, all play a crucial function within this system. Stomach dysrhythmias, which are linked to problems with the movement of gastrointestinal contents, affect\u0000 a significant number of individuals all over the globe. These problems include inappropriate digestion (dyspepsia), nausea (vomiting sensation) for no apparent reason, vomiting, abdominal pain, stomach ulcers, gastroesophageal reflux disease, and other disorders. During the process of finding\u0000 the anomalies, it is possible that a number of techniques, including as imaging, endoscopy, electrogastrogram, and clinical analysis, will be used. Electrogastrography signals, also known as electrogastrograms (EGG), were captured using surface Ag/AgCl electrodes that were put over the stomach\u0000 in 20 healthy persons before the data was gathered and pre-processed. The datasets were produced from these signals (8 Females and 12 Males). In addition to this, the datasets were obtained from 10 individuals who were suffering from various stomach illnesses (3 Females and 8 Males). In the\u0000 stage known as “pre-processing,” which needs the obtained dataset to be treated in advance, any noise that was present in the signal is removed. In order to rid the data of any noise and increase the overall quality of the input data, a technique that is known as the Wiener filter\u0000 is used. A technique known as Hybrid Grey Wolf Optimization with Particle Swarm Optimization is utilized in the process of feature selection. This algorithm is responsible for removing any extraneous data from the features that have been collected from the signal. The procedure is sped up\u0000 as a result of this. The classifiers get the qualities that have been chosen as their input in order to carry out an analysis of the many stomach disorders, such as primary gastric lymphoma, gastrointestinal stromal tumour (GIST), and neuroendocrine tumor. This enables the classifiers to do\u0000 the analysis (carcinoid). The Multi-class Feed Forward Neural Network Classifier (MCFFN) is used to carry out the classification process. This classifier provides the stages along with the classes. The accuracy, sensitivity, and specificity of the classification process are taken into account\u0000 in the calculation of performance measures.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48469451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Retinal ganglion cells (RGC) axons participate in the construction of optic nerve, and prevent the damage of RGC during acute optic nerve injury. IncRNA-XIST is crucial for RGC apoptosis. Our study intends to assess IncRNA-XIST’s role in the regulation of RGC apoptosis in an attempt to provide a theoretical basis for treating optic nerve crush injury. Two genotypes of mice (wild-type and miR-36 KO) were used to establish an optic nerve crush injury model to investigate the regulatory role of IncRNA-XIST gene in RGCs apoptosis. These mice were then randomly assigned into control group (WT), injury group, and XIST/injury group. The changes of apoptotic genes and proteins in retinal ganglion cells were analyzed by qPCR, WB and TUNEL staining. In wild-type mice, RGC apoptosis was significantly increased after optic nerve compression injury, and the expression of Bax and Bad was significantly increased. When the LncRNA-XIST gene was overexpressed before retinal crush injury, the apoptosis of retinal ganglion cells was significantly reduced, and Bax and Bad levels were decreased as compared with model group of optic nerve injury. The results showed that in wild-type mice, overexpression of IncRNA-XIST gene promoted the survival of RGC after optic nerve crush injury. In addition, upregulation of IncRNA-xist expression in miR-36 KO mice did not reduce retinal ganglion cell apoptosis and alter the apoptotic protein expression after optic nerve crush injury. Defects of miR-36 alone or overexpression of XIST gene do not cause morphological damage of retina in mice. In mouse ganglion cells, miR-36 expression was up-regulated in both injured cells and overexpressed XIST gene. However, up-regulation of miR-36 caused by overexpression of XIST gene was more obvious. In addition, in vivo studies of wild-type mice, it was found that overexpression of XIST reduced retinal ganglion cell apoptosis, and this effect was abolished in miR-36 KO mice. In conclusion, lncRNA-XIST reduces ganglion cell apoptosis by upregulating miR-36 and promotes the survival of RGC after nerve crush injury.
{"title":"Long Non-Coding RNA-X-Inactive Specific Transcript Promotes the Retinal Ganglion Cell Survival After Optic Nerve Crush Injury by Upregulating miR-36","authors":"Qingjia Ren, Junjun Zhang","doi":"10.1166/jbt.2023.3265","DOIUrl":"https://doi.org/10.1166/jbt.2023.3265","url":null,"abstract":"Retinal ganglion cells (RGC) axons participate in the construction of optic nerve, and prevent the damage of RGC during acute optic nerve injury. IncRNA-XIST is crucial for RGC apoptosis. Our study intends to assess IncRNA-XIST’s role in the regulation of RGC apoptosis in an attempt\u0000 to provide a theoretical basis for treating optic nerve crush injury. Two genotypes of mice (wild-type and miR-36 KO) were used to establish an optic nerve crush injury model to investigate the regulatory role of IncRNA-XIST gene in RGCs apoptosis. These mice were then randomly assigned into\u0000 control group (WT), injury group, and XIST/injury group. The changes of apoptotic genes and proteins in retinal ganglion cells were analyzed by qPCR, WB and TUNEL staining. In wild-type mice, RGC apoptosis was significantly increased after optic nerve compression injury, and the expression\u0000 of Bax and Bad was significantly increased. When the LncRNA-XIST gene was overexpressed before retinal crush injury, the apoptosis of retinal ganglion cells was significantly reduced, and Bax and Bad levels were decreased as compared with model group of optic nerve injury. The results showed\u0000 that in wild-type mice, overexpression of IncRNA-XIST gene promoted the survival of RGC after optic nerve crush injury. In addition, upregulation of IncRNA-xist expression in miR-36 KO mice did not reduce retinal ganglion cell apoptosis and alter the apoptotic protein expression after optic\u0000 nerve crush injury. Defects of miR-36 alone or overexpression of XIST gene do not cause morphological damage of retina in mice. In mouse ganglion cells, miR-36 expression was up-regulated in both injured cells and overexpressed XIST gene. However, up-regulation of miR-36 caused by overexpression\u0000 of XIST gene was more obvious. In addition, in vivo studies of wild-type mice, it was found that overexpression of XIST reduced retinal ganglion cell apoptosis, and this effect was abolished in miR-36 KO mice. In conclusion, lncRNA-XIST reduces ganglion cell apoptosis by upregulating\u0000 miR-36 and promotes the survival of RGC after nerve crush injury.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47489385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Feng Qiu, Leyi Xu, Li Gong, Lingjun Kong, Jue Zhang, Z. Fei
This study discusses the mechanism of miR-512-5p derived from BMSC in restraining the proliferation and prompting apoptosis of GBM. BMSC exosome was obtained through ultra-centrifugation and assessed by TEM. The positive presentation of CD63 and HSP70 was detected with Western Blot. The GBM cell line LN229 was divided into WM set, NC set, and ZR set followed by analysis of cell proliferation by MTT method, invasive ability by Tranwell chamber, apoptotic rate by FCM and the expression of JAG1 and notch2 by Western Blot. miR-512-5p level in LN229 cells was significantly lower than U87MG and SHG44 cells. There was positive expression of CD63 and HSP70 in exosome. LN229 cell proliferation was restrained by the drug. ZR set had lower cell proliferation rate and invasive quantity and higher apoptotic rate than WM set and NC set. The protein expressions of JAG1 and notch2 in ZR set was reduced compared with WM set and NC set (P <0.05) without difference between NC set and WM set (P >0.05). In conclusion, GBM cell proliferation could be restrained and apoptosis could be increased by miR-512-5p derived from BMSC through targeting JAG1. It could provide a brand-new therapeutic strategy for the treatment on GBM.
{"title":"Bone Marrow Mesenchymal Stem Cells (BMSC) Exosome-Derived mR-512-5p Inhibits the Proliferation and Apoptosis of Glioblastoma Cells","authors":"Feng Qiu, Leyi Xu, Li Gong, Lingjun Kong, Jue Zhang, Z. Fei","doi":"10.1166/jbt.2023.3266","DOIUrl":"https://doi.org/10.1166/jbt.2023.3266","url":null,"abstract":"This study discusses the mechanism of miR-512-5p derived from BMSC in restraining the proliferation and prompting apoptosis of GBM. BMSC exosome was obtained through ultra-centrifugation and assessed by TEM. The positive presentation of CD63 and HSP70 was detected with Western Blot.\u0000 The GBM cell line LN229 was divided into WM set, NC set, and ZR set followed by analysis of cell proliferation by MTT method, invasive ability by Tranwell chamber, apoptotic rate by FCM and the expression of JAG1 and notch2 by Western Blot. miR-512-5p level in LN229 cells was significantly\u0000 lower than U87MG and SHG44 cells. There was positive expression of CD63 and HSP70 in exosome. LN229 cell proliferation was restrained by the drug. ZR set had lower cell proliferation rate and invasive quantity and higher apoptotic rate than WM set and NC set. The protein expressions of JAG1\u0000 and notch2 in ZR set was reduced compared with WM set and NC set (P <0.05) without difference between NC set and WM set (P >0.05). In conclusion, GBM cell proliferation could be restrained and apoptosis could be increased by miR-512-5p derived from BMSC through targeting\u0000 JAG1. It could provide a brand-new therapeutic strategy for the treatment on GBM.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"122 3","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41284489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liu-Jie Gao, Ting-Ting Shao, Wan-Zhen Zheng, Jiguo Ding, Jun Li
MicroRNAs (miRs) have been proved to play significant role in both the tumorigenesis and recurrence of lung cancer (LA). However, the identification of which miR participated in the recurrence of LA, as well as the underlying mechanism are poorly understood. Here in our article, quantitative analysis of the miR-100 expression in non-recurrent and recurrent LA tissues using real-time PCR (RT-qPCR) revealed that, compared to non-recurrent LA, the expression of miR-100 was significantly downregulated in recurrent ones. Especially, the chemokine (CXC motif) receptor 7 (CXCR7) level was negatively correlated with the expression of miR-100 in both non-recurrent and recurrent LA cell lines derived from corresponding LA tissues. The following in vitro experiments also confirmed that either miR-100 overexpression or CXCR7 depletion decreased proliferation of recurrent LA cells. In conclusion, miR-100 may act as a recurrence suppressor in LA through the regulation of CXCR7 expression, which might be a promising target for the prevention of LA recurrence.
{"title":"Downregulated miR-100 Result in High Expression Levels of Chemokine (CXC Motif) Receptor 7 That Predict Recurrence of Lung Cancer","authors":"Liu-Jie Gao, Ting-Ting Shao, Wan-Zhen Zheng, Jiguo Ding, Jun Li","doi":"10.1166/jbt.2023.2619","DOIUrl":"https://doi.org/10.1166/jbt.2023.2619","url":null,"abstract":"MicroRNAs (miRs) have been proved to play significant role in both the tumorigenesis and recurrence of lung cancer (LA). However, the identification of which miR participated in the recurrence of LA, as well as the underlying mechanism are poorly understood. Here in our article, quantitative\u0000 analysis of the miR-100 expression in non-recurrent and recurrent LA tissues using real-time PCR (RT-qPCR) revealed that, compared to non-recurrent LA, the expression of miR-100 was significantly downregulated in recurrent ones. Especially, the chemokine (CXC motif) receptor 7 (CXCR7) level\u0000 was negatively correlated with the expression of miR-100 in both non-recurrent and recurrent LA cell lines derived from corresponding LA tissues. The following in vitro experiments also confirmed that either miR-100 overexpression or CXCR7 depletion decreased proliferation of recurrent\u0000 LA cells. In conclusion, miR-100 may act as a recurrence suppressor in LA through the regulation of CXCR7 expression, which might be a promising target for the prevention of LA recurrence.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42200246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Urine from domestic animals has traditionally been used to treat multiple human diseases. Rock hyrax hyraceum (RHH) is a dark brown resin-like substance made up of accumulated feces and urine of rock hyraxes, used in traditional African medicine to treat snake and scorpion bites, as well as colic, hysteria, epilepsy, and Parkinson’s disease. As there is no scientific backing for the utilization of hyrax hyraceum, there is an urgent need to establish its safety as a medicine. Furthermore, toxicity evaluation facilitates the identification of factors for clinical surveillance of the potential side effects, as well as the estimation of a preliminary hygienic dose level and dosing frequency range for human clinical trials. The safety of RHH was assessed by administering 2.5 and 5 gm/kg doses of hyraceum crystals dissolved in de-ionized water and administered to rats orally. We evaluated the acute toxicity profile, as well as the effect of RHH on liver and kidney function and histopathological investigations. The results of this investigation showed that consuming RHH does not have any hazardous effects on liver and kidney function and histopathological investigations. Drinking RHH did not cause toxicity. These findings are consistent with those of previous studies of drinking cows and camel urine. We encourage more research on hyraceum composition in relation to rock hyrax habitat areas and eating patterns, as we believe it will help us understand why people practice drinking hyraceum as a medicine.
{"title":"Safety Evaluation of Rock Hyrax (Procavia capensis) Hyraceum: Oral Toxicity Study in Rats","authors":"Manei M. Aljedaie, Mohamed A. Akela","doi":"10.1166/jbt.2023.3270","DOIUrl":"https://doi.org/10.1166/jbt.2023.3270","url":null,"abstract":"Urine from domestic animals has traditionally been used to treat multiple human diseases. Rock hyrax hyraceum (RHH) is a dark brown resin-like substance made up of accumulated feces and urine of rock hyraxes, used in traditional African medicine to treat snake and scorpion bites, as\u0000 well as colic, hysteria, epilepsy, and Parkinson’s disease. As there is no scientific backing for the utilization of hyrax hyraceum, there is an urgent need to establish its safety as a medicine. Furthermore, toxicity evaluation facilitates the identification of factors for clinical\u0000 surveillance of the potential side effects, as well as the estimation of a preliminary hygienic dose level and dosing frequency range for human clinical trials. The safety of RHH was assessed by administering 2.5 and 5 gm/kg doses of hyraceum crystals dissolved in de-ionized water and administered\u0000 to rats orally. We evaluated the acute toxicity profile, as well as the effect of RHH on liver and kidney function and histopathological investigations. The results of this investigation showed that consuming RHH does not have any hazardous effects on liver and kidney function and histopathological\u0000 investigations. Drinking RHH did not cause toxicity. These findings are consistent with those of previous studies of drinking cows and camel urine. We encourage more research on hyraceum composition in relation to rock hyrax habitat areas and eating patterns, as we believe it will help us\u0000 understand why people practice drinking hyraceum as a medicine.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48407663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kaiyu Li, Chunbo Li, Jin-Chao Zhao, Xin Ge, Nan Wang, Yu Sun
LINC02532 and miR-362-5p modulates gastric cancer (GC) cell activities. Herein, we elucidated the role of LINC02532 targeting miR-362-5p to mediate p-AKT in GC cells, aiming to provide a theoretical basis for clinical treatment. Human GC cells were treated with si-LINC02532, si-NC, LINC02532+miR-362-5p inhibitor and p-AKT inhibitor. LINC02532 and miR-362-5p expression was determined by RT-qPCR and p-AKT expression was detected. Transwell assay assessed cell invasion and migration upon treatment and the targeting relationship of LINC02532 and miR-362-5p was evaluated. A positive expression of LINC02532 and miR-362-5p was detected in each group of GC cells. The expression of LINC02532 was up-regulated (2.95 ± 0.23) and miR-362-5p was down-regulated (0.35 ± 0.08). Silence of LINC02532 significantly suppressed GC cell behaviors and inhibited migration speed of cancer cells, while p-AKT inhibitor treatment resulted in a decrease in the number of invaded and migrated cells. Combination of LINC02532 and miR-362-5p inhibitor was not effective as previous two treatments, but still decreased cell migration and invasion (p < 0.05). The luciferase experiment indicated LINC02532 targeted miR-362-5p. Down-regulation of LINC02532 also reduced p-AKT protein expression. p-AKT inhibitor group had a lower level of p-AKT protein, followed by LINC02532+miR-362-5p inhibitor group, and si-NC group. In conclusion, silence of LINC02532 reduces miR-362-5p and p-AKT protein expression in GC cells to suppress GC cell growth through inhibition of p-AKT signaling pathway.
{"title":"Long Non-Coding RNA LINC02532 Mediates p-AKT to Regulate Gastric Cancer Cell Activities Through Targeting miR-362-5p","authors":"Kaiyu Li, Chunbo Li, Jin-Chao Zhao, Xin Ge, Nan Wang, Yu Sun","doi":"10.1166/jbt.2023.3271","DOIUrl":"https://doi.org/10.1166/jbt.2023.3271","url":null,"abstract":"LINC02532 and miR-362-5p modulates gastric cancer (GC) cell activities. Herein, we elucidated the role of LINC02532 targeting miR-362-5p to mediate p-AKT in GC cells, aiming to provide a theoretical basis for clinical treatment. Human GC cells were treated with si-LINC02532, si-NC,\u0000 LINC02532+miR-362-5p inhibitor and p-AKT inhibitor. LINC02532 and miR-362-5p expression was determined by RT-qPCR and p-AKT expression was detected. Transwell assay assessed cell invasion and migration upon treatment and the targeting relationship of LINC02532 and miR-362-5p was evaluated.\u0000 A positive expression of LINC02532 and miR-362-5p was detected in each group of GC cells. The expression of LINC02532 was up-regulated (2.95 ± 0.23) and miR-362-5p was down-regulated (0.35 ± 0.08). Silence of LINC02532 significantly suppressed GC cell behaviors and inhibited\u0000 migration speed of cancer cells, while p-AKT inhibitor treatment resulted in a decrease in the number of invaded and migrated cells. Combination of LINC02532 and miR-362-5p inhibitor was not effective as previous two treatments, but still decreased cell migration and invasion (p <\u0000 0.05). The luciferase experiment indicated LINC02532 targeted miR-362-5p. Down-regulation of LINC02532 also reduced p-AKT protein expression. p-AKT inhibitor group had a lower level of p-AKT protein, followed by LINC02532+miR-362-5p inhibitor group, and si-NC group. In conclusion, silence\u0000 of LINC02532 reduces miR-362-5p and p-AKT protein expression in GC cells to suppress GC cell growth through inhibition of p-AKT signaling pathway.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46968075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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