Journal of chemical technology and biotechnology最新文献_第6页

Novel nanoparticle-mediated plasmid delivery in bacteria for difficult applications 新型纳米颗粒介导的质粒递送在细菌中的困难应用

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology

Pub Date : 2025-09-18 DOI: 10.1002/jctb.70070

Laura D. Páez-Angarita, Laura X. Henao, Sebastián Jaramillo-Toro, Valentina Quezada, Silvia Restrepo, Juan C. Cruz, Johana Husserl

BACKGROUND

This study presents the development and application of magnetite/silver-pDMAEMA-PEG-BUFII nanobioconjugates as an advanced plasmid delivery system in bacteria. These nanobioconjugates demonstrated superior efficiency compared to traditional methods in four challenging applications: (i) genomic editing in Gram-positive bacteria; (ii) CRISPR/Cas9-mediated antibiotic resistance control; (iii) large plasmid delivery; and (iv) transformation in complex environmental matrices such as soil.

RESULTS

Functionalization with pDMAEMA improved plasmid loading, while PEGylation enhanced stability, dispersity, and cellular uptake. The system enabled high-efficiency transformation in Streptomyces JH010, overcoming low conjugation efficiency observed with conventional approaches. In Escherichia coli, the nanobioconjugates facilitated effective CRISPR/Cas9-based resensitization to antibiotics, achieving higher transformation and genome-editing efficiencies than traditional competent-cell methods. Furthermore, the nanobioconjugates achieved large plasmid delivery without requiring conjugation or phage-based methods, thereby simplifying transformation procedures. In soil environments, plasmid transfer was significantly enhanced compared to transformation in chemically/competent cells, demonstrating potential for bioremediation and environmental applications. Characterization confirmed successful multi-functionalization, enhanced colloidal stability, and low toxicity across bacterial models. The magnetic properties of the nanoparticles further enable potential recovery and reuse, reducing environmental impact.

CONCLUSION

These findings highlight the versatility and robustness of nanobioconjugate-mediated plasmid delivery, providing an effective alternative for bacterial genetic engineering in medical, industrial, and environmental settings. Future research should focus on optimizing delivery conditions for diverse bacterial species, scaling up for industrial applications, and assessing long-term impacts in complex biological systems. © 2025 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

本研究介绍了磁铁矿/银- pdmaema - peg - bufii纳米生物偶联物作为一种先进的质粒递送系统在细菌中的开发和应用。与传统方法相比，这些纳米生物偶联物在四个具有挑战性的应用中表现出更高的效率：(i)革兰氏阳性细菌的基因组编辑；（ii） CRISPR/ cas9介导的抗生素耐药性控制；（iii）质粒量大；（iv）在土壤等复杂环境基质中的转化。结果pDMAEMA功能化提高了质粒的负载，而PEGylation增强了稳定性、分散性和细胞摄取。该系统克服了传统方法偶联效率低的问题，实现了Streptomyces JH010的高效转化。在大肠杆菌中，纳米生物偶联物促进了基于CRISPR/ cas9的抗生素重敏，实现了比传统的活性细胞方法更高的转化和基因组编辑效率。此外，纳米生物偶联物在不需要偶联或基于噬菌体的方法的情况下实现了大型质粒递送，从而简化了转化过程。在土壤环境中，质粒的转移比在化学/能态细胞中的转化显著增强，显示出生物修复和环境应用的潜力。表征证实了成功的多功能化，增强了胶体稳定性，并且在细菌模型中具有低毒性。纳米颗粒的磁性能进一步实现潜在的回收和再利用，减少对环境的影响。结论这些发现强调了纳米生物偶联物介导的质粒传递的多功能性和稳健性，为医学、工业和环境环境中的细菌基因工程提供了有效的替代方案。未来的研究应侧重于优化不同细菌种类的递送条件，扩大工业应用规模，以及评估复杂生物系统的长期影响。©2025作者。由John Wiley &； Sons Ltd代表美国化学工业学会（SCI）出版的化学技术与生物技术杂志。

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引用次数: 0

Removal of ship borne oil from seawater using Maltodextrin – gum Arabic – aerogel biocomposites: characterization, equilibrium, kinetics, thermodynamics, and process optimization 用麦芽糖糊精-阿拉伯胶-气凝胶生物复合材料从海水中去除船载油：表征、平衡、动力学、热力学和工艺优化

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology

Pub Date : 2025-09-16 DOI: 10.1002/jctb.70069

Noor Khader Hussain Hussain, Berrin Saygi, Ahmet Alper Aydın, Didem Saloglu

BACKGROUND

Numerous contemporary investigations on the physical, chemical, thermal, and biological processes used to treat oil from seawater. The majority of the approaches' primary drawback, though, is how challenging it is to modify them for direct field use in traditional treatment systems. Of them, the adsorption technique is the most easily adapted to traditional water treatment systems. In this context, the presented paper investigates maltodextrin-gum arabic-silica aerogel biocomposites as ship-borne oil adsorbent from seawater performance and feasibility.

Results

The 10%, 20%, and 30% gum arabic embedded in 10% maltodextrin and 2% silica aerogel synthesized biocomposites ship-borne oil removal % values were 13.97%, 11.83%, and 28.57%, respectively. Experimental data by nonlinear isotherm and kinetics models of Langmuir, Freundlich, Temkin, and Dubinin-Radushkevich and nonlinear kinetic models of pseudo first-order, pseudo second-order, Weber Morris, and Elovich were evaluated using STATISTICA (Version 8.0, StatSoft Inc., USA). Higher correlation coefficients (R²) and lower chi-square (χ²) represented that Freundlich and Temkin isotherm models and Weber Morris and pseudo first-order kinetic models were superior for ship-borne oil adsorption. The positive enthalpy revealed endothermic oil adsorption. The optimum independent variables resulted in a ship-borne oil removal onto maltodextrin-gum arabic-aerogel with 2.91 g/g adsorption capacity.

Conclusion

The data from the first investigation in the literature on the adsorption of maltodextrin-gum arabic-aerogel biocomposites for the removal of ship-borne oil from seawater are presented in this work. © 2025 Society of Chemical Industry (SCI).

背景：目前对处理海水中石油的物理、化学、热和生物工艺进行了大量的研究。然而，大多数方法的主要缺点是，将它们修改为直接在传统处理系统中使用是多么具有挑战性。其中，吸附技术最容易适应传统的水处理系统。在此背景下，本文研究了麦芽糖糊精-阿拉伯胶-二氧化硅气凝胶生物复合材料作为船用海水油吸附剂的性能和可行性。结果10%、20%、30%阿拉伯胶包埋在10%麦精糊精和2%二氧化硅气凝胶合成的生物复合材料中，其船载除油率分别为13.97%、11.83%和28.57%。采用STATISTICA （Version 8.0, StatSoft Inc.， USA）软件对Langmuir、Freundlich、Temkin和Dubinin-Radushkevich非线性等温线和动力学模型以及伪一阶、伪二阶、Weber Morris和Elovich非线性动力学模型的实验数据进行评估。较高的相关系数（R2）和较低的χ2 （χ2）表明Freundlich和Temkin等温模型以及Weber Morris和伪一级动力学模型对船舶油的吸附效果较好。正焓表明吸热油吸附。最佳自变量结果表明，麦芽糖糊精-阿拉伯胶-气凝胶的船舶除油性能为2.91 g/g。本文首次研究了麦芽糖糊精-阿拉伯胶-气凝胶生物复合材料对海水中船舶载油的吸附作用。©2025化学工业学会（SCI）。

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引用次数: 0

Navigating the landscape of microbial fuel cell bioelectrochemical technology: a review from current to potential applications 微生物燃料电池生物电化学技术的前景：从目前到潜在的应用综述

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology

Pub Date : 2025-09-15 DOI: 10.1002/jctb.70064

Abdulrahman Itopa Suleiman, Claudia Guerrero-Barajas, Mustapha Omenesa Idris, Abdulrazaq Itopa Ahmad, Abdulganiu Adeiza Otuoze, Barnabas Ojochegbe Akuba, Agada Isaac Eva, David Adeiza Zakari, Abdulbasit Anoze Aliyu, Abdulrahaman Okino Otuoze

The globe will continue to confront major issues with environmental sustainability and energy in the years to come. As a result, it is crucial to pursue sustainable development to protect the environment and make the most of renewable energy sources. One promising approach involves using natural microorganisms to generate energy from affordable substrates through Microbial Fuel Cells (MFCs). These bioelectrochemical systems work by harnessing the ability of microbes to break down organic materials. This process releases electrons that travel through an external circuit, producing power. This review explores the basic principles and concepts behind MFCs, while also considering the key factors affecting their performance. The review provides a detailed analysis of MFCs, focusing on the characteristics and functions of exoelectrogens and the optimal conditions necessary for their efficient operation. It also discusses the working principles and mechanisms of MFCs, including recent advancements in the field. The primary focus of the review is on the potential and practical applications of this sustainable energy technology. It details various real-world uses, including biosensors, electronic devices, desalination, and environmental bioremediation. © 2025 Society of Chemical Industry (SCI).

未来几年，全球将继续面临环境可持续性和能源方面的重大问题。因此，保护环境和充分利用可再生能源是实现可持续发展的关键。一种有前景的方法是利用天然微生物通过微生物燃料电池（mfc）从可负担得起的基质中产生能量。这些生物电化学系统的工作原理是利用微生物分解有机物质的能力。这一过程释放出电子，电子通过外部电路，产生电力。本文探讨了mfc背后的基本原理和概念，同时也考虑了影响其性能的关键因素。本文对mfc进行了详细的分析，重点介绍了其产电特性和功能，以及其高效运行所需的最佳条件。它还讨论了mfc的工作原理和机制，包括该领域的最新进展。审查的主要重点是这种可持续能源技术的潜力和实际应用。它详细介绍了各种现实世界的应用，包括生物传感器、电子设备、海水淡化和环境生物修复。©2025化学工业学会（SCI）。

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引用次数: 0

In situ adsorption and controlled dosing of sodium dodecylsulfate enhances bacterial violacein production: Insights into kinetics, cell morphology and integrated process design 原位吸附和控制剂量十二烷基硫酸钠提高细菌紫色素生产：洞察动力学，细胞形态和综合工艺设计

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology

Pub Date : 2025-09-15 DOI: 10.1002/jctb.70072

Krushna Gharat, Rekha S Singhal

BACKGROUND

Despite having immense therapeutic potential, violacein, a bacterial metabolite, lacks industrial exploration owing to low titer and compromised yields. Current study explores integrated in situ strategies for enhanced biomanufacturing of violacein. In situ strategies involve simultaneous release and recovery of metabolites from biological systems while keeping the system viable for further production.

RESULTS

Sodium dodecylsulfate (SDS) at a preoptimized dose of 0.005% (w/v) in the broth was selected as a permeabilizing agent for elevating the release of violacein from Chromobacterium violaceum MTCC2656. SDS modulated the cell morphology which was assessed by biochemical assays and transmission electron microscopy. SP200 was selected as an adsorbent after multi-level screening based on the requirements of in situ recovery operations. The interactions of violacein and SP200 showed pseudo-first-order kinetics of adsorption and were best fitted with Langmuir's adsorption isotherm. Insights from these studies were applied to design a process for in situ recovery of violacein from broth and improved the overall titers and productivity of violacein from 7.34 to 14.63 mg⁻¹ L⁻¹ h⁻¹.

CONCLUSION

Inducing cell permeability by appropriate dosing of surfactant and in situ adsorption emerges as a successful integrated approach for enhanced biomanufacturing of violacein due to enhanced productivity as well as ease of downstream processing. © 2025 Society of Chemical Industry (SCI).

背景：紫紫素作为一种细菌代谢物，尽管具有巨大的治疗潜力，但由于其低效价和低收率而缺乏工业开发。目前的研究探索了加强紫罗兰素生物制造的综合原位策略。就地策略包括从生物系统中同时释放和回收代谢物，同时保持系统对进一步生产的可行性。结果经筛选，以0.005% （w/v）十二烷基硫酸钠（SDS）为渗透剂，可促进紫色色杆菌MTCC2656中紫色素的释放。SDS对细胞形态的调节作用通过生化实验和透射电镜观察。根据现场开采作业的要求，经过多级筛选，选择SP200作为吸附剂。紫紫素与SP200的相互作用表现出准一级吸附动力学，符合Langmuir吸附等温线。从这些研究中获得的见解被应用于设计一种从肉汤中原位回收紫罗兰素的工艺，并将紫罗兰素的总体滴度和产率从7.34提高到14.63 mg−1 L−1 h−1。结论通过适当剂量的表面活性剂和原位吸附诱导细胞通透性是一种成功的提高紫罗兰素生物生产效率和易于下游加工的综合方法。©2025化学工业学会（SCI）。

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引用次数: 0

Experimental evaluation of sequencing batch reactor with continuous feeding: a novel continuous batch process for wastewater treatment 连续进料序批式反应器的实验评价：一种新型连续批式污水处理工艺

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology

Pub Date : 2025-09-13 DOI: 10.1002/jctb.70062

Derin Orhon, Esra Hallaç, Bülent Solmaz, Seval Sözen

BACKGROUND

This study experimentally tested and evaluated the organic carbon (COD) removal performance of a sequencing batch reactor (SBR) with continuous sewage feeding, to define a novel process scheme, the continuous batch reactor (CBR). The experiments involved two pilot plants: a CBR unit, and a conventional SBR unit with the same reactor volume, which operated with two cycles per day and intermittent feeding, for parallel evaluation. The study emphasized the effect of continuous feeding on COD removal and challenged the empirical practice of intermittent feeding.

RESULTS

The effluent COD of the CBR unit remained within the bracket of 50–70 mg L⁻¹, with an average value of 67 mg L⁻¹, while the influent COD exhibited a significant fluctuation between 400 and 600 mg L⁻¹. The performance of the parallel SBR unit was quite similar. The cyclic soluble COD profile of the CBR unit was lowered down to a plateau right after entering the reactor, and remained constant throughout the process phase, as it primarily consisted of soluble inert COD fractions. The incremental sewage feeding during the idle phase exerted a minor increase in the effluent soluble COD level.

CONCLUSION

The novel CBR scheme eliminated the need for multiple parallel reactors associated with conventional SBR systems operated with intermittent and short feeding periods. Continuous feeding, inherently avoided in conventional SBR operation, did not appreciably affect system performance. It also provided ideal operating conditions for aeration and complete substrate removal, since the incremental sewage feeding into the entire biomass would maintain a very low substrate-to-biomass ratio, leading to immediate substrate adsorption and utilization. © 2025 Society of Chemical Industry (SCI).

本研究通过对连续进料污水的顺序间歇反应器（SBR）的有机碳（COD）去除性能进行实验测试和评价，以确定一种新的工艺方案——连续间歇反应器（CBR）。实验涉及两个试验装置：一个CBR装置和一个具有相同反应器容量的传统SBR装置，该装置以每天两个循环和间歇投料的方式运行，以进行平行评估。本研究强调了连续投喂对COD去除的影响，对间歇投喂的经验做法提出了挑战。结果CBR出水COD保持在50 ~ 70 mg L−1的范围内，平均值为67 mg L−1，进水COD在400 ~ 600 mg L−1之间波动较大。并联SBR装置的性能非常相似。CBR装置的循环可溶性COD剖面在进入反应器后立即降至平台，并在整个工艺阶段保持不变，因为它主要由可溶性惰性COD组分组成。在空闲阶段增加污水进给量对出水可溶性COD水平有轻微的增加。结论新型CBR方案消除了传统SBR系统中间歇和短投料周期运行的多个并联反应器的需要。常规SBR操作固有地避免了连续进料，对系统性能没有明显影响。它还为曝气和完全去除底物提供了理想的操作条件，因为进入整个生物质的增量污水将保持非常低的底物与生物质的比例，从而导致底物立即吸附和利用。©2025化学工业学会（SCI）。

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引用次数: 0

Pectin-bentonite-zeolite bead for encapsulation of corn mint (Mentha arvensis) essential oil: surface structural characterization and adsorption–desorption properties 玉米薄荷精油包封用果胶-膨润土-沸石珠：表面结构表征及吸附-解吸性能

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology

Pub Date : 2025-09-13 DOI: 10.1002/jctb.70068

Lai Minh Nhat Anh, Le Tan Phong, Nguyen Thi Truc Phuong, Nguyen Van Dung, Tran Thuy Tuyet Mai, Ngo Tran Hoang Duong, Nguyen Quang Long

Background

Mentha arvensis essential oil exhibits strong insecticidal activity against stored-grain pests but suffers from high volatility and poor stability. Conventional nano-encapsulation improves stability but often yields formulations that are not ready-to-use, limiting practical applications. This study aimed to address these challenges by encapsulating corn mint essential oil into granulated zeolite X and Mordenite structures using pectin and bentonite as binders.

Results

The prepared beads were highly porous, spherical, and had a uniform diameter of approximately 3 mm. Their mechanical strength, which depended on the type of zeolite used, decreased slightly after the encapsulation process. The granulation process preserved the structural characteristics of zeolite X and zeolite MOR, allowing the pectin-bentonite-zeolite beads to effectively adsorb menthol and menthone – the primary active compounds in corn mint oil, as confirmed by GC–MS analysis. Based on TGA results, the loading capacity and encapsulation efficiency of calcined beads made with zeolite X were calculated to be approximately 12.4% and 41.33%, respectively. Under all chosen temperature points, the R² values were greater than 0.97 for all samples, so the two-stage kinetic model is best suitable for explaining the desorption of corn mint essential oil within pectin-bentonite-zeolite beads.

Conclusions

This work presents the first granulated zeolite-based carrier for corn mint essential oil, overcoming the limitations of powder formulations. The approach enhances stability, facilitates handling and packaging, and holds promise for sustainable pest management applications. © 2025 Society of Chemical Industry (SCI).

背景薄荷精油对储粮害虫具有较强的杀虫活性，但挥发性高，稳定性差。传统的纳米封装提高了稳定性，但通常产生的配方不是现成的，限制了实际应用。本研究旨在解决这些挑战，通过将玉米薄荷精油包封到粒状沸石X和丝光沸石结构中，使用果胶和膨润土作为粘合剂。结果制备的微球多孔，呈球形，直径均匀，约为3mm。它们的机械强度在包封后略有下降，这取决于所使用的沸石类型。GC-MS分析证实，造粒过程保留了X沸石和MOR沸石的结构特征，使果胶-膨润土-沸石珠能够有效吸附薄荷油中的主要活性成分薄荷醇和薄荷酮。根据TGA结果，计算出X沸石煅烧球的负载能力和包封效率分别约为12.4%和41.33%。在所有选择的温度点下，所有样品的R2值都大于0.97，因此两阶段动力学模型最适合解释玉米薄荷精油在果胶-膨润土-沸石珠内的解吸。结论本文首次建立了玉米薄荷精油颗粒状载体，克服了粉末状载体的局限性。该方法提高了稳定性，便于处理和包装，并有望实现可持续虫害管理应用。©2025化学工业学会（SCI）。

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引用次数: 0

Dissolution and separation of carbon dioxide in biohydrogen by monoethanolamine-based deep eutectic solvents 单乙醇胺基深共晶溶剂对生物氢中二氧化碳的溶解和分离

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology

Pub Date : 2025-09-12 DOI: 10.1002/jctb.70059

Xiaokai Zhou, Yanyan Jing, Cunjie Li, Quanguo Zhang, Yameng Li, Tian Zhang, Kai Zhang

Background

The presence of CO₂ in biohydrogen reduces hydrogen purity, thereby limiting its application in fuel cells, hydrogen-enriched water and other advanced energy sectors. To improve biohydrogen purity, the solubility of pure CO₂ under different molar ratios, temperatures and aqueous deep eutectic solvents (DESs) concentrations were studied, and a response surface optimization experiment was carried out. Subsequently, aqueous DESs were utilized to investigate the decarbonization and purification of biohydrogen at varying temperatures and biohydrogen flow rates.

Results

The results demonstrated that the DESs composed of acetamide and monoethanolamine (A-MEA) exhibited effective CO₂ solubility properties at 65 °C. Upon the addition of water, the viscosity of A-MEA was significantly reduced, improving its fluidity. Notably, the optimum CO₂ dissolution temperature of aqueous A-MEA decreased to 40 °C. Optimal purification performance was achieved when biohydrogen was passed through 40% aqueous A-MEA at a flow rate of 100 mL min⁻¹. Under these conditions, hydrogen purity increased from 60.00% to over 99.00% and was sustained for 21 min. Simultaneously, the CO₂-dissolving capacity of the solvent reached 0.311 g CO₂ g⁻¹ A-MEA.

Conclusion

Aqueous A-MEA is an efficient CO₂ absorbent that provides important insights for advancing biohydrogen purification and laying the foundation for its future industrial-scale application. © 2025 Society of Chemical Industry (SCI).

生物氢中二氧化碳的存在降低了氢的纯度，从而限制了其在燃料电池、富氢水和其他先进能源领域的应用。为了提高生物氢的纯度，研究了纯CO2在不同摩尔比、温度和水深共晶溶剂（DESs）浓度下的溶解度，并进行了响应面优化实验。随后，利用水相DESs研究了不同温度和不同氢流量下生物氢的脱碳和纯化。结果由乙酰胺和单乙醇胺（A-MEA）组成的DESs在65℃下具有良好的CO2溶解度。加入水后，A-MEA的粘度明显降低，流动性提高。值得注意的是，A-MEA的最佳CO2溶解温度降至40℃。当生物氢以100 mL min - 1的流速通过40%的a - mea时，纯化效果最佳。在此条件下，氢的纯度从60.00%提高到99.00%以上，并持续21 min。同时，该溶剂的CO2溶出量达到0.311 g CO2 g−1 A-MEA。结论A-MEA是一种高效的CO2吸收剂，为推进生物氢净化提供了重要见解，为其未来的工业规模应用奠定了基础。©2025化学工业学会（SCI）。

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引用次数: 0

Design and evaluation of a novel two-phase partitioning bioreactor to enhance methane mass transfer 新型甲烷传质两相分配生物反应器的设计与评价

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology

Pub Date : 2025-09-12 DOI: 10.1002/jctb.70063

Robert Bertrand, Lisa Stephanie Dizon, William Holmes, Dhan Fortela, Andrei Chistoserdov, Rafael Hernandez, Mark Zappi, Emmanuel Revellame

Background

Methane is typically used in combustion reactions for energy generation or as a disposal strategy. The latter is an environmentally unsustainable practice that demands alternative utilization strategies to convert methane into high-value products. Bioconversion is a responsible methane conversion practice that utilizes bacteria to directly metabolize methane. However, due to mass transfer limitations, widespread adoption of this technology is limited. Thus, a novel two-phase partitioning bioreactor (TPPB) was constructed to improve methane transport through a circulating non-aqueous phase (NAP). Silicone, mineral, and soybean oils were tested abiotically for their suitability as NAPs by evaluating methane volumetric mass transfer coefficients (k_La) at different circulation flow rates (0.25–1.50 L/min).

Results

Due to emulsion formation at higher circulation rates, soybean oil was only tested at 0.25–1.00 L/min, which showed insignificant k_La improvement. For mineral oil, the circulation rate significantly improved the average methane k_La from 9.46 h⁻¹ (0.25 L/min) to 20.44 h⁻¹ (1.5 L/min). Conversely, silicone oil showed the highest significant k_La improvement when the circulation flow rate increased from 0.5 L/min (k_La = 9.93 ± 1.77 h⁻¹) to 1.25 L/min (k_La = 49.24 ± 3.04 h⁻¹). Compared to direct gaseous methane bubbling, 1.25 L/min silicone oil provided a 900% k_La enhancement per unit mass flow of methane fed into the TPPB.

Conclusion

This study demonstrated that TPPB with circulating NAP can provide significantly higher methane k_La than direct methane injection. Although the present study focused solely on abiotic evaluation, the TPPB could prove beneficial in advancing the bioconversion of methane into useful materials. © 2025 Society of Chemical Industry (SCI).

甲烷通常用于燃烧反应以产生能量或作为一种处理策略。后者是一种环境上不可持续的做法，需要其他利用战略将甲烷转化为高价值产品。生物转化是一种负责任的甲烷转化实践，利用细菌直接代谢甲烷。然而，由于传质的限制，该技术的广泛采用受到限制。因此，构建了一种新型的两相分配生物反应器（TPPB），以改善甲烷通过循环非水相（NAP）的输送。在不同循环流速（0.25 ~ 1.50 L/min）下，通过评估甲烷体积传质系数（kLa），对硅油、矿物油和大豆油作为nap的适用性进行了非生物测试。结果大豆油在较高的循环速率下形成乳化液，仅在0.25 ~ 1.00 L/min的循环速率下检测，kLa改善不显著。对于矿物油，循环速率显著提高了平均甲烷kLa，从9.46 h−1 （0.25 L/min）提高到20.44 h−1 （1.5 L/min）。相反，当循环流量从0.5 L/min （kLa = 9.93±1.77 h−1）增加到1.25 L/min （kLa = 49.24±3.04 h−1）时，硅油的kLa改善最为显著。与直接气体甲烷鼓泡相比，1.25 L/min的硅油可使进入TPPB的甲烷单位质量流量增加900% kLa。结论循环NAP的TPPB提供的甲烷kLa明显高于直接注甲烷。虽然目前的研究仅集中在非生物评价上，但TPPB可以证明有利于推进甲烷转化为有用材料的生物转化。©2025化学工业学会（SCI）。

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引用次数: 0

Enrichment of algae harvesting and actions of potassium carbonate on hydrogen production from dairy wastewater via supercritical water gasification: performance measures 藻类富集及碳酸钾对乳品废水超临界水气化制氢的作用：性能指标

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology

Pub Date : 2025-09-11 DOI: 10.1002/jctb.70065

A Mohana Krishnan, N Nagabhooshanam, Yogendra Thakur, Ankur Kulshreshta, MD Anto Praveena, UL Nagendra Kumar, Debabrata Barik, R Srinivasan, S Sathiyamurthy

BACKGROUND

The reliance on non-renewable fossil fuels is prevalent across numerous energy sectors, significantly contributing to global warming. However, the emergence of hydrogen energy technologies presents a promising opportunity for achieving environmental sustainability. By offering carbon-free emissions, hydrogen could revolutionize our approach to energy, becoming a vital carrier for the future. The main aim of the study was to produce hydrogen energy from dairy wastewater featuring 0.1, 0.2, 0.3, and 0.4 vol% of iron oxide (Fe₂O₃) through a 14 wt% potassium carbonate (K₂CO₃)-configured supercritical water gasification (SCWG) process, followed by 400–600 °C gasification temperature with 25 bar pressure maintained by 30 min residence time. The impact of Fe₂O₃ concentration on algae growth and harvesting efficiency was measured over consecutive days.

RESULTS

The 0.4 vol% Fe₂O₃ concentration facilitates superior algae growth of 0.85 μm d⁻¹ and improved harvesting efficiency by 89%. The optimal algae growth from dairy waste/0.4 vol% Fe₂O₃ was considered a biomass feedstock for the SCWG process. The influence of an alkaline catalyst with 14 wt% K₂CO₃ and varying gasification temperatures on molar fraction percentage, hydrogen selectivity, hydrogen yield, and gasification efficiency was experimentally studied. The outcomes demonstrated that the alkaline catalyst with 14 wt% K₂CO₃, configured for SCWG, functioned at 600 °C, achieving 58% hydrogen percentage, 18.5% hydrogen selectivity, and 23.65 mol kg⁻¹ of hydrogen yield with a maximum gasification efficiency of 88%. The extracted hydrogen energy is suitable for alternative energy applications.

CONCLUSION

The dual role of the Fe₂O₃ and K₂CO₃ featured gasification process found improved algal growth and superior syngas production (higher hydrogen).

许多能源部门普遍依赖不可再生的化石燃料，这对全球变暖起到了重要作用。然而，氢能源技术的出现为实现环境可持续性提供了一个有希望的机会。通过提供无碳排放，氢可以彻底改变我们的能源方式，成为未来的重要载体。该研究的主要目的是通过14 wt%碳酸钾（K2CO3）配置的超临界水气化（SCWG）工艺，在400-600°C的气化温度下，在25 bar的压力下保持30分钟的停留时间，从含有0.1、0.2、0.3和0.4 vol%氧化铁（Fe2O3）的乳制品废水中生产氢能源。连续数天测量Fe2O3浓度对藻类生长和收获效率的影响。结果当Fe2O3浓度为0.4 vol%时，藻类生长速度达到0.85 μm d−1，收获效率提高89%。以乳酸渣/0.4 vol% Fe2O3为原料的最佳藻类生长被认为是SCWG工艺的生物质原料。实验研究了K2CO3质量分数为14wt %的碱性催化剂和不同气化温度对摩尔分数、氢气选择性、产氢率和气化效率的影响。结果表明，在600℃条件下，K2CO3质量分数为14wt %的碱性催化剂可实现58%的氢气率、18.5%的氢气选择性和23.65 mol kg−1的氢气产率，气化效率最高可达88%。提取的氢能适合替代能源的应用。结论在Fe2O3和K2CO3的双重作用下，气化过程可以促进藻类生长，提高合成气产量（氢气含量更高）。

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引用次数: 0

Advanced process design for large-scale de-novo 2-phenylethanol production via fermentation 大规模发酵生产2-苯乙醇的先进工艺设计

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology

Pub Date : 2025-09-10 DOI: 10.1002/jctb.70057

Tamara Janković, Tobias Fecker, Jean-Marc Daran, Adrie J.J. Straathof, Anton A. Kiss

BACKGROUND

2-Phenylethanol (2PE) is a valuable aroma component that can be obtained through de-novo fermentation from glucose. However, its toxicity at very low concentrations (<2.5 g L⁻¹) limits the fermentation titer, rate and yield. To address these limitations, in-situ product removal has been explored, leading to a recent scale-up to pilot scale. Nonetheless, an industrial scale has yet to be achieved.

RESULTS

This original research pioneers conceptual development of two large-scale (2 ktonne_2PE/y) production processes for 2PE via de-novo fermentation from glucose. Liquid–liquid extraction with oleyl alcohol and adsorption by hydrophobic resins followed by ethanol desorption, were alternatives considered for in-situ 2PE removal. For either design, solvent recovery and final purification were performed using advanced distillation techniques, including a heat pump-assisted distillation and a dividing-wall column. A fermentation titer of approximately 1.5 g_2PE/L_broth minimized production costs by achieving balance between upstream and downstream processing costs. This resulted in a cost-effective 2PE production for both designs of the recovery process (9.03–9.40 $/kg_2PE). Sensitivity analysis revealed that glucose, oleyl alcohol, and ethanol costs strongly impact total production costs.

CONCLUSION

This novel study provides a comprehensive and scalable process framework for the large-scale production of 2PE through de-novo fermentation. Integrating in-situ product removal and energy-efficient purification strategies, it marks a significant step forward in industrial biotechnology. © 2025 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

背景2-苯乙醇（2PE）是一种有价值的香气成分，可以通过葡萄糖去新发酵获得。然而，其毒性在非常低的浓度（<2.5 g L−1）限制了发酵滴度、速率和产量。为了解决这些限制，研究人员探索了原位产物去除技术，并将其扩大到中试规模。尽管如此，工业规模尚未达到。结果：本研究开创了两个大规模（2 ktonne2PE/年）通过葡萄糖从头发酵生产2PE的概念发展。油醇液液萃取和疏水树脂吸附后乙醇解吸是原位去除2PE的两种方法。对于这两种设计，溶剂回收和最终纯化使用先进的蒸馏技术，包括热泵辅助蒸馏和分壁塔。发酵滴度约为1.5 g2PE/Lbroth通过实现上游和下游加工成本之间的平衡来最小化生产成本。这使得两种回收工艺的2PE生产都具有成本效益（9.03-9.40美元/kg2PE）。敏感性分析显示，葡萄糖、油醇和乙醇成本对总生产成本影响很大。结论本研究为通过去novo发酵大规模生产2PE提供了一个全面的、可扩展的工艺框架。它结合了原位产物去除和节能净化策略，标志着工业生物技术向前迈出了重要一步。©2025作者。由John Wiley &； Sons Ltd代表美国化学工业学会（SCI）出版的化学技术与生物技术杂志。

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引用次数: 0