Chae-Eun Kim, Seung-Hyun Park, Kyeong-Won Paeng, Lorenzo Tavelli, David T. Wu, Daniel S. Thoma, Ronald E. Jung, Ui-Won Jung, Jae-Kook Cha
Aim
To test the hypothesis that a volume-stable collagen matrix (VCMX) can serve as an effective rhBMP-2 carrier for bone regeneration while compensating for the volume instability of absorbable collagen sponge (ACS).
Materials and Methods
(i) rhBMP-2 release kinetics from VCMX and ACS were measured over 7 days by enzyme-linked immunosorbent assay. (ii) Bilateral sinus augmentations were performed in 15 rabbits using either VCMX or ACS, with or without rhBMP-2. After 4 weeks, radiographic and histological analyses were conducted.
Results
The overall release patterns between VCMX and ACS did not differ significantly, although VCMX showed a delayed peak release (at 3 h) compared to ACS (at 10 min). Three-dimensional radiographic analysis showed greater volumetric gains in VCMX groups than in ACS groups, regardless of rhBMP-2 loading. Histologically, VCMX/rhBMP-2 group exhibited the largest total augmented area (13.1 ± 2.9 mm2). The use of rhBMP-2 led to significant increases in new bone area for both VCMX (3.9 ± 1.1 vs. 1.6 ± 1.3 mm2, p = 0.003) and ACS (1.8 ± 0.8 vs. 1.0 ± 0.6 mm2, p = 0.035). Notably, the proportion of new bone in the total augmented region was comparable between the VCMX/rhBMP-2 and ACS/rhBMP-2 groups (p > 0.05).
Conclusion
rhBMP-2-loaded VCMX enhances bone regeneration in sinus augmentation, suggesting that its volume maintenance may contribute to the improved clinical and radiographic outcomes.
目的验证一种体积稳定的胶原基质（VCMX）可以作为骨再生的有效载体，同时补偿可吸收胶原海绵（ACS）的体积不稳定性。材料和方法(i)采用酶联免疫吸附法测定VCMX和ACS在7天内释放rhBMP - 2的动力学。（ii） 15只兔使用VCMX或ACS进行双侧鼻窦增强，有或没有rhBMP‐2。4周后，进行影像学和组织学分析。结果VCMX与ACS的整体释放模式无显著差异，但VCMX的峰值释放延迟（3 h），而ACS的峰值释放延迟（10 min）。三维x线分析显示，无论rhBMP - 2负荷如何，VCMX组比ACS组的体积增加更大。组织学上，VCMX/rhBMP‐2组表现出最大的总增强面积（13.1±2.9 mm2）。rhBMP‐2的使用导致VCMX（3.9±1.1 vs. 1.6±1.3 mm2, p = 0.003）和ACS（1.8±0.8 vs. 1.0±0.6 mm2, p = 0.035）的新骨面积显著增加。值得注意的是，VCMX/rhBMP‐2组和ACS/rhBMP‐2组在总增强区的新骨比例相当（p > 0.05）。结论负载rhbmp - 2的VCMX增强了窦性骨再生，表明其体积维持可能有助于改善临床和影像学结果。
{"title":"Volume-Stable Collagen Matrix as a Recombinant Human Bone Morphogenetic Protein-2 Carrier for Maxillary Sinus Augmentation: An Experimental In Vivo Study","authors":"Chae-Eun Kim, Seung-Hyun Park, Kyeong-Won Paeng, Lorenzo Tavelli, David T. Wu, Daniel S. Thoma, Ronald E. Jung, Ui-Won Jung, Jae-Kook Cha","doi":"10.1111/jcpe.70054","DOIUrl":"10.1111/jcpe.70054","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To test the hypothesis that a volume-stable collagen matrix (VCMX) can serve as an effective rhBMP-2 carrier for bone regeneration while compensating for the volume instability of absorbable collagen sponge (ACS).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>(i) rhBMP-2 release kinetics from VCMX and ACS were measured over 7 days by enzyme-linked immunosorbent assay. (ii) Bilateral sinus augmentations were performed in 15 rabbits using either VCMX or ACS, with or without rhBMP-2. After 4 weeks, radiographic and histological analyses were conducted.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The overall release patterns between VCMX and ACS did not differ significantly, although VCMX showed a delayed peak release (at 3 h) compared to ACS (at 10 min). Three-dimensional radiographic analysis showed greater volumetric gains in VCMX groups than in ACS groups, regardless of rhBMP-2 loading. Histologically, VCMX/rhBMP-2 group exhibited the largest total augmented area (13.1 ± 2.9 mm<sup>2</sup>). The use of rhBMP-2 led to significant increases in new bone area for both VCMX (3.9 ± 1.1 vs. 1.6 ± 1.3 mm<sup>2</sup>, <i>p</i> = 0.003) and ACS (1.8 ± 0.8 vs. 1.0 ± 0.6 mm<sup>2</sup>, <i>p</i> = 0.035). Notably, the proportion of new bone in the total augmented region was comparable between the VCMX/rhBMP-2 and ACS/rhBMP-2 groups (<i>p</i> > 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>rhBMP-2-loaded VCMX enhances bone regeneration in sinus augmentation, suggesting that its volume maintenance may contribute to the improved clinical and radiographic outcomes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 2","pages":"299-307"},"PeriodicalIF":6.8,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145314581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sophie Koehlen, Inga Harks, Johannes Matern, Karola Prior, Peter Eickholz, Katrin Lorenz, Ti-Sun Kim, Thomas Kocher, Jörg Meyle, Doğan Kaner, Yvonne Jockel-Schneider, Dag Harmsen, Benjamin Ehmke, Sven Kleine Bardenhorst, Daniel Hagenfeld
Aim
To investigate whether exhaled carbon monoxide (CO) is dose-dependently associated with subgingival polymicrobial clusters before and after non-surgical periodontal therapy.
Material and Methods
We followed 163 adults with periodontitis, measuring CO before and at 2, 14 and 26 months after therapy. Subgingival plaque samples were analysed using 16S rRNA gene sequencing. We applied topic models and regression analysis, adjusted for age, sex, treatment group, pocket probing depth and plaque levels.
Results
Smokers had generally higher mean CO levels at every visit and showed significant declines at 14 and 26 months after therapy (p < 0.01). Before therapy, CO was not associated with dysbiosis; after therapy, higher levels were positively associated. In sensitivity analyses, self-reported smoking status explained more variance in dysbiosis than CO alone, with only marginal gains when both were combined. Among smokers, a distinct polymicrobial cluster dominated by Fusobacterium and Prevotella increased with higher exhaled CO. Every 10 parts per million increase in CO was associated with an estimated 16% increase in cluster abundance (95% confidence interval: 8%–26%).
Conclusion
Exhaled CO may help monitor persistent dysbiosis and select an anaerobe-dominated subgingival community after periodontal therapy among smokers.
{"title":"Exhaled Carbon Monoxide Indicates Persistent Subgingival Dysbiosis After Periodontal Therapy","authors":"Sophie Koehlen, Inga Harks, Johannes Matern, Karola Prior, Peter Eickholz, Katrin Lorenz, Ti-Sun Kim, Thomas Kocher, Jörg Meyle, Doğan Kaner, Yvonne Jockel-Schneider, Dag Harmsen, Benjamin Ehmke, Sven Kleine Bardenhorst, Daniel Hagenfeld","doi":"10.1111/jcpe.70053","DOIUrl":"10.1111/jcpe.70053","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To investigate whether exhaled carbon monoxide (CO) is dose-dependently associated with subgingival polymicrobial clusters before and after non-surgical periodontal therapy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>We followed 163 adults with periodontitis, measuring CO before and at 2, 14 and 26 months after therapy. Subgingival plaque samples were analysed using 16S rRNA gene sequencing. We applied topic models and regression analysis, adjusted for age, sex, treatment group, pocket probing depth and plaque levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Smokers had generally higher mean CO levels at every visit and showed significant declines at 14 and 26 months after therapy (<i>p</i> < 0.01). Before therapy, CO was not associated with dysbiosis; after therapy, higher levels were positively associated. In sensitivity analyses, self-reported smoking status explained more variance in dysbiosis than CO alone, with only marginal gains when both were combined. Among smokers, a distinct polymicrobial cluster dominated by <i>Fusobacterium</i> and <i>Prevotella</i> increased with higher exhaled CO. Every 10 parts per million increase in CO was associated with an estimated 16% increase in cluster abundance (95% confidence interval: 8%–26%).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Exhaled CO may help monitor persistent dysbiosis and select an anaerobe-dominated subgingival community after periodontal therapy among smokers.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 2","pages":"170-178"},"PeriodicalIF":6.8,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Simranjit Kaur, Peter Riis Hansen, Claus Henrik Nielsen, Palle Holmstrup, Anne Havemose-Poulsen, Merete Markvart, Nikoline Nygaard, Christian Damgaard
Aim
To determine (i) the parameters used by dental professionals to evaluate patients with periodontitis, (ii) the prevalence of periodontitis using different case definitions and (iii) associations between periodontitis and diabetes mellitus, cardiovascular disease and rheumatoid arthritis among adult patients in private practices in Denmark.
Materials and Methods
This nationwide descriptive study used data collected from private practices using the DentalSuite electronic dental record system between 2000 and 2022. Patients 18 years or older with a Danish social security number were included in the study. Prevalences of periodontitis were calculated using periodontal registrations and different case definitions for periodontitis. Registrations of selected comorbidities along with periodontitis, namely diabetes mellitus, cardiovascular diseases and rheumatoid arthritis, were also collected.
Results
A total of 1,473,428 individuals were included in the study, of whom 782,464 (53%) were females. About 302,604 (20.5%) patients complied with the periodontal probing depth ≥ 5 mm PD case definition. Furthermore, 130,688 (8.9%) patients had periodontal parameters that fulfilled the more specific 2018 AAP/EFP classification. Periodontal probing depth registrations were available for 24% of the patients. Patients identified with periodontitis had higher prevalences of the selected comorbidities compared to the population as a whole.
Conclusions
In private practices in Denmark, probing pocket depth was the most frequently registered periodontal parameter used for screening and monitoring periodontitis. The selected comorbidities appeared to be more prevalent in patients with periodontitis.
{"title":"Descriptive Analysis of Periodontitis Case Detection and Selected Comorbidities in Danish Private Dental Practices","authors":"Simranjit Kaur, Peter Riis Hansen, Claus Henrik Nielsen, Palle Holmstrup, Anne Havemose-Poulsen, Merete Markvart, Nikoline Nygaard, Christian Damgaard","doi":"10.1111/jcpe.70046","DOIUrl":"10.1111/jcpe.70046","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To determine (i) the parameters used by dental professionals to evaluate patients with periodontitis, (ii) the prevalence of periodontitis using different case definitions and (iii) associations between periodontitis and diabetes mellitus, cardiovascular disease and rheumatoid arthritis among adult patients in private practices in Denmark.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>This nationwide descriptive study used data collected from private practices using the DentalSuite electronic dental record system between 2000 and 2022. Patients 18 years or older with a Danish social security number were included in the study. Prevalences of periodontitis were calculated using periodontal registrations and different case definitions for periodontitis. Registrations of selected comorbidities along with periodontitis, namely diabetes mellitus, cardiovascular diseases and rheumatoid arthritis, were also collected.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 1,473,428 individuals were included in the study, of whom 782,464 (53%) were females. About 302,604 (20.5%) patients complied with the periodontal probing depth ≥ 5 mm PD case definition. Furthermore, 130,688 (8.9%) patients had periodontal parameters that fulfilled the more specific 2018 AAP/EFP classification. Periodontal probing depth registrations were available for 24% of the patients. Patients identified with periodontitis had higher prevalences of the selected comorbidities compared to the population as a whole.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>In private practices in Denmark, probing pocket depth was the most frequently registered periodontal parameter used for screening and monitoring periodontitis. The selected comorbidities appeared to be more prevalent in patients with periodontitis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 2","pages":"214-221"},"PeriodicalIF":6.8,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Craig S. Miller, Qi Yan, Sreenatha S. Kirakodu, Jeffrey L. Ebersole, Xiaohua D. Zhang
Aim
This secondary analysis of a cross-sectional study tested the hypothesis that a salivary biomarker panel (i.e., consisting of 2–6 features) could accurately identify periodontitis in persons with Type 2 diabetes (T2DM) compared with non-periodontitis in systemically healthy persons.
Materials and Methods
Salivary concentrations of 12 protein biomarkers and 14 oral microbiome species were evaluated by immunoassays and 16S rRNA sequencing, respectively, from 28 systemically healthy non-periodontitis adults and 28 T2DM patients with periodontitis. Data were analysed for the identification of periodontitis from non-periodontitis using 5-fold cross-validation logistic regression, receiver operating characteristics (ROC) and odds ratios.
Results
Bacteria showed better predictive value than individual salivary proteins. Two bacteria (Porphyromonas gingivalis and Mycoplasma faucium) yielded specificities > 90%, Prevotella species yielded high sensitivity (86%) and Treponema socranskii demonstrated the top area under the curve (AUC) (0.81). A salivary panel consisting of bacteria (Selenomonas sputigena, P. gingivalis, Prevotella nigrescens, Pr. dentalis) and protein ratios (prostaglandin E2/tissue inhibitor of metalloproteinase-1 or macrophage inflammatory protein-1α/tissue inhibitor of metalloproteinase-1) produced robust diagnostic accuracy (95%) and precision (96.6%) for the detection of periodontitis in T2DM.
Conclusions
A salivary panel using bacteria and ratios of host-response biomarkers accurately identified periodontitis in T2DM compared with systemically healthy persons without periodontitis.
{"title":"Salivary Biomarker Panel That Identifies Periodontitis in Persons With Type 2 Diabetes: A Secondary Analysis of a Cross-Sectional Study","authors":"Craig S. Miller, Qi Yan, Sreenatha S. Kirakodu, Jeffrey L. Ebersole, Xiaohua D. Zhang","doi":"10.1111/jcpe.70049","DOIUrl":"10.1111/jcpe.70049","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>This secondary analysis of a cross-sectional study tested the hypothesis that a salivary biomarker panel (i.e., consisting of 2–6 features) could accurately identify periodontitis in persons with Type 2 diabetes (T2DM) compared with non-periodontitis in systemically healthy persons.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Salivary concentrations of 12 protein biomarkers and 14 oral microbiome species were evaluated by immunoassays and 16S rRNA sequencing, respectively, from 28 systemically healthy non-periodontitis adults and 28 T2DM patients with periodontitis. Data were analysed for the identification of periodontitis from non-periodontitis using 5-fold cross-validation logistic regression, receiver operating characteristics (ROC) and odds ratios.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Bacteria showed better predictive value than individual salivary proteins. Two bacteria (<i>Porphyromonas gingivalis</i> and <i>Mycoplasma faucium</i>) yielded specificities > 90%, <i>Prevotella species</i> yielded high sensitivity (86%) and <i>Treponema socranskii</i> demonstrated the top area under the curve (AUC) (0.81). A salivary panel consisting of bacteria (<i>Selenomonas sputigena</i>, <i>P. gingivalis</i>, <i>Prevotella nigrescens</i>, <i>Pr. dentalis</i>) and protein ratios (prostaglandin E2/tissue inhibitor of metalloproteinase-1 or macrophage inflammatory protein-1α/tissue inhibitor of metalloproteinase-1) produced robust diagnostic accuracy (95%) and precision (96.6%) for the detection of periodontitis in T2DM.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>A salivary panel using bacteria and ratios of host-response biomarkers accurately identified periodontitis in T2DM compared with systemically healthy persons without periodontitis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 2","pages":"190-200"},"PeriodicalIF":6.8,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Dionigi, A. C. Ericson, A. Shikhan, L. Larsson, T. Berglundh
Aim
To analyse gene expression profiles in human tissue samples obtained from dental implant sites with or without peri-implantitis.
Materials and Methods
Soft-tissue biopsy samples obtained from patients presenting with implants with severe peri-implantitis and adjacent reference implants with no signs of peri-implantitis were analysed by spatial transcriptomics (n = 4), RNA-sequencing (n = 20) and immunohistochemistry (n = 38).
Results
The analysis disclosed evident associations between distinct gene clusters and specific tissue compartments in both groups of specimens. Samples from peri-implantitis sites showed higher levels of gene activity than samples from reference implant sites. Gene-set enrichment analysis revealed that several important biological pathways, for example, ‘regulation of antimicrobial host response’, ‘collagen fibril organisation’ and ‘regulation of angiogenesis’, were severely dysregulated in peri-implantitis lesions when compared to reference implant sites. Similarly, immunohistochemical evaluation showed elevated levels of proteins corresponding to the most up-regulated, differentially expressed genes (DEGs), which were identified by spatial transcriptomics and RNA-sequencing.
Conclusions
Peri-implant lesions demonstrated associations between gene clusters and specific tissue compartments. Several biological pathways linked to the activation of host response towards bacterial insults were dysregulated in peri-implantitis specimens when compared to reference implant samples.
{"title":"Spatial Transcriptomic Assessments of Gene Expression Profiles in Human Peri-Implant Lesions","authors":"C. Dionigi, A. C. Ericson, A. Shikhan, L. Larsson, T. Berglundh","doi":"10.1111/jcpe.70008","DOIUrl":"10.1111/jcpe.70008","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To analyse gene expression profiles in human tissue samples obtained from dental implant sites with or without peri-implantitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Soft-tissue biopsy samples obtained from patients presenting with implants with severe peri-implantitis and adjacent reference implants with no signs of peri-implantitis were analysed by spatial transcriptomics (<i>n</i> = 4), RNA-sequencing (<i>n</i> = 20) and immunohistochemistry (<i>n</i> = 38).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The analysis disclosed evident associations between distinct gene clusters and specific tissue compartments in both groups of specimens. Samples from peri-implantitis sites showed higher levels of gene activity than samples from reference implant sites. Gene-set enrichment analysis revealed that several important biological pathways, for example, ‘regulation of antimicrobial host response’, ‘collagen fibril organisation’ and ‘regulation of angiogenesis’, were severely dysregulated in peri-implantitis lesions when compared to reference implant sites. Similarly, immunohistochemical evaluation showed elevated levels of proteins corresponding to the most up-regulated, differentially expressed genes (DEGs), which were identified by spatial transcriptomics and RNA-sequencing.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Peri-implant lesions demonstrated associations between gene clusters and specific tissue compartments. Several biological pathways linked to the activation of host response towards bacterial insults were dysregulated in peri-implantitis specimens when compared to reference implant samples.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 1","pages":"137-151"},"PeriodicalIF":6.8,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hélvis E. S. Paz, Mabelle F. Monteiro, Camila S. Stolf, Cássia F. Araújo, Angelika Silbereisen, Mauro P. Santamaria, Nagihan Bostanci, Renato C. V. Casarin
Aim
To investigate whether periodontitis in parents is associated with differences in the faecal microbiome and systemic markers in both themselves and their children.
Methods
Eighty participants were divided into four groups (n = 20): parents with periodontitis (PP); healthy parents (PC); and their respective children (CP, CC). Clinical periodontal parameters were recorded. Saliva and faecal bacterial DNA were analysed via 16S rRNA sequencing. Salivary lactoferrin, faecal calprotectin, gingival crevicular fluid cytokines (IFN-γ, IL-10, IL-17, IL-1β, IL-4, TNF-α) and urinary intestinal permeability markers (claudin-2, -3, -4, haptoglobin) were quantified.
Results
Parents with periodontitis showed distinct faecal microbiota profiles, which were mirrored in their children and significantly differed from controls. Claudin-2 levels were elevated in both PP and CP groups (p < 0.05) and positively correlated with the oral dysbiosis index and the faecal Firmicutes/Bacteroidetes ratio.
Conclusions
Parental periodontal health appears to influence the faecal microbiome and systemic markers in the offspring. These findings highlight a potential pathway for oral–gut microbial transmission and its relevance to systemic health, warranting further investigation.
{"title":"Familial Patterns of Oral–Gut Dysbiosis and Systemic Markers in Periodontitis","authors":"Hélvis E. S. Paz, Mabelle F. Monteiro, Camila S. Stolf, Cássia F. Araújo, Angelika Silbereisen, Mauro P. Santamaria, Nagihan Bostanci, Renato C. V. Casarin","doi":"10.1111/jcpe.70047","DOIUrl":"10.1111/jcpe.70047","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To investigate whether periodontitis in parents is associated with differences in the faecal microbiome and systemic markers in both themselves and their children.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Eighty participants were divided into four groups (<i>n</i> = 20): parents with periodontitis (PP); healthy parents (PC); and their respective children (CP, CC). Clinical periodontal parameters were recorded. Saliva and faecal bacterial DNA were analysed via 16S rRNA sequencing. Salivary lactoferrin, faecal calprotectin, gingival crevicular fluid cytokines (IFN-γ, IL-10, IL-17, IL-1β, IL-4, TNF-α) and urinary intestinal permeability markers (claudin-2, -3, -4, haptoglobin) were quantified.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Parents with periodontitis showed distinct faecal microbiota profiles, which were mirrored in their children and significantly differed from controls. Claudin-2 levels were elevated in both PP and CP groups (<i>p</i> < 0.05) and positively correlated with the oral dysbiosis index and the faecal Firmicutes/Bacteroidetes ratio.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Parental periodontal health appears to influence the faecal microbiome and systemic markers in the offspring. These findings highlight a potential pathway for oral–gut microbial transmission and its relevance to systemic health, warranting further investigation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 2","pages":"222-231"},"PeriodicalIF":6.8,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wentao Zhu, Kathy Fung, Pawan Dhami, Paul Sharpe, Jan Krivanek, Luigi Nibali, Cheng Zhang, Vitor C. M. Neves
Aim
To investigate the cellular composition and molecular mechanisms of periodontal granulation tissue formation using single-cell RNA sequencing (scRNA-seq), aiming to enhance the understanding of periodontal disease pathogenesis and identify potential targets for regenerative therapies.
Materials and Methods
Granulation tissue samples were collected from patients undergoing periodontal surgery (n = 3). Fresh tissues were processed into single-cell suspensions and subjected to scRNA-seq. The data were integrated and compared with existing datasets from healthy gingiva and periodontal ligament. Computational analyses were performed and validated through immunofluorescence staining.
Results
Ten distinct cell clusters were identified across the samples. Granulation tissue exhibited a higher abundance of immune cells compared to healthy tissues. A novel endothelial cell subpopulation, exclusive to granulation tissue, was discovered and characterised by NOTCH3 expression and involvement in ossification pathways. Additionally, granulation tissue fibroblast subpopulations demonstrated a progenitor-like state, characterised by extracellular matrix reorganisation and low differentiation, similar to cancer-associated fibroblasts.
Conclusion
This study identified a novel endothelial subpopulation offering new insights into the disease's pathogenesis and presenting potential targets for regenerative therapies. These findings will help advance the understanding of periodontal disease granulation tissue formation and provide information for the development of materials to modulate specific cellular pathways to improve periodontal disease management.
{"title":"Characterising the Periodontal Granulation Tissue Using scRNAseq","authors":"Wentao Zhu, Kathy Fung, Pawan Dhami, Paul Sharpe, Jan Krivanek, Luigi Nibali, Cheng Zhang, Vitor C. M. Neves","doi":"10.1111/jcpe.70048","DOIUrl":"10.1111/jcpe.70048","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To investigate the cellular composition and molecular mechanisms of periodontal granulation tissue formation using single-cell RNA sequencing (scRNA-seq), aiming to enhance the understanding of periodontal disease pathogenesis and identify potential targets for regenerative therapies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Granulation tissue samples were collected from patients undergoing periodontal surgery (<i>n</i> = 3). Fresh tissues were processed into single-cell suspensions and subjected to scRNA-seq. The data were integrated and compared with existing datasets from healthy gingiva and periodontal ligament. Computational analyses were performed and validated through immunofluorescence staining.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Ten distinct cell clusters were identified across the samples. Granulation tissue exhibited a higher abundance of immune cells compared to healthy tissues. A novel endothelial cell subpopulation, exclusive to granulation tissue, was discovered and characterised by <i>NOTCH3</i> expression and involvement in ossification pathways. Additionally, granulation tissue fibroblast subpopulations demonstrated a progenitor-like state, characterised by extracellular matrix reorganisation and low differentiation, similar to cancer-associated fibroblasts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study identified a novel endothelial subpopulation offering new insights into the disease's pathogenesis and presenting potential targets for regenerative therapies. These findings will help advance the understanding of periodontal disease granulation tissue formation and provide information for the development of materials to modulate specific cellular pathways to improve periodontal disease management.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 2","pages":"308-320"},"PeriodicalIF":6.8,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lina Weinert, Nihad El Sayed, Michel Wensing, Bettina Dannewitz, Stefan Listl
Aim
The objectives of this study were to gain a comprehensive understanding of patients' barriers to adherence to periodontal treatment and to derive intervention functions for a digital companion to support them through their therapy journey.
Materials and Methods
A qualitative study incorporating patient and expert interviews was designed. Data were analysed using thematic analysis. Barriers to therapy adherence and functions for a digital intervention were synthesised with the help of the Capability, Opportunity and Motivation behaviour change model.
Results
Barriers related to periodontal therapy are a lack of awareness of the patients' own responsibility and prioritisation of individual oral health, lack of time to both instruct as well as learn and perform better oral hygiene and lack of knowledge about disease characteristics and ideal habits. Participants expected that a digital intervention should incorporate personalised and comprehensive educational material, possibilities to visually document progress, simple descriptions of oral hygiene tasks, reminders and the inclusion of prompts for self-reflection.
Conclusion
While a digital health companion has the potential to address the aforementioned barriers, future research should also aim to develop strategies to focus on health systems barriers (e.g., financing and workforce arrangements) as these can influence patient engagement and adherence.
{"title":"Exploring Barriers to Periodontal Treatment Adherence in Patients With Periodontitis to Inform Development of a Digital Companion: A Qualitative Study","authors":"Lina Weinert, Nihad El Sayed, Michel Wensing, Bettina Dannewitz, Stefan Listl","doi":"10.1111/jcpe.70044","DOIUrl":"10.1111/jcpe.70044","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The objectives of this study were to gain a comprehensive understanding of patients' barriers to adherence to periodontal treatment and to derive intervention functions for a digital companion to support them through their therapy journey.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A qualitative study incorporating patient and expert interviews was designed. Data were analysed using thematic analysis. Barriers to therapy adherence and functions for a digital intervention were synthesised with the help of the Capability, Opportunity and Motivation behaviour change model.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Barriers related to periodontal therapy are a lack of awareness of the patients' own responsibility and prioritisation of individual oral health, lack of time to both instruct as well as learn and perform better oral hygiene and lack of knowledge about disease characteristics and ideal habits. Participants expected that a digital intervention should incorporate personalised and comprehensive educational material, possibilities to visually document progress, simple descriptions of oral hygiene tasks, reminders and the inclusion of prompts for self-reflection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>While a digital health companion has the potential to address the aforementioned barriers, future research should also aim to develop strategies to focus on health systems barriers (e.g., financing and workforce arrangements) as these can influence patient engagement and adherence.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 1","pages":"58-71"},"PeriodicalIF":6.8,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hélvis E. S. Paz, Camila S. Stolf, Kai Bao, Mabelle F. Monteiro, Lucas M. Carvalho, Angelika Silbereisen, Georgios N. Belibasakis, Renato C. V. Casarin, Nagihan Bostanci
Aim
To evaluate the subgingival proteome and microbiome of mothers with periodontitis and their offspring, thereby assessing signatures of periodontal diseases.
Methods
Forty participants in four groups were included: mothers with periodontitis and their offspring, as well as periodontally healthy mothers and their offspring. Periodontal clinical parameters were assessed. Gingival crevicular fluid (GCF) and subgingival biofilm were collected from the same sites. Proteome from GCF was investigated by liquid chromatography-tandem mass spectrometry with data-independent acquisition (DIA-PASEF). Bacterial DNA from subgingival biofilms was sequenced using the 16S rRNA gene for taxonomy assignment.
Results
Overall, 6147 bacterial and human proteins (≥ 2 peptides) were quantified. Despite the absence of attachment loss, the offspring of mothers with periodontitis presented with similar proteotypes as their mothers, characterised by up-regulation of inflammatory response cascades and down-regulation of epithelial barrier proteins. They also displayed higher colonisation patterns by periodontopathogens while presenting with increased expression of bacterial virulence proteins compared with controls.
Conclusion
The study showed that the maternal periodontal microbiome and proteome associate with those of the offspring and relate to maternal periodontal status. These early ecological events may potentially promote offspring's susceptibility to dysbiosis and may predispose them to periodontitis.
{"title":"The Subgingival Host–Microbial Landscape in Mothers With Periodontitis and Their At-Risk Offspring","authors":"Hélvis E. S. Paz, Camila S. Stolf, Kai Bao, Mabelle F. Monteiro, Lucas M. Carvalho, Angelika Silbereisen, Georgios N. Belibasakis, Renato C. V. Casarin, Nagihan Bostanci","doi":"10.1111/jcpe.70045","DOIUrl":"10.1111/jcpe.70045","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To evaluate the subgingival proteome and microbiome of mothers with periodontitis and their offspring, thereby assessing signatures of periodontal diseases.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Forty participants in four groups were included: mothers with periodontitis and their offspring, as well as periodontally healthy mothers and their offspring. Periodontal clinical parameters were assessed. Gingival crevicular fluid (GCF) and subgingival biofilm were collected from the same sites. Proteome from GCF was investigated by liquid chromatography-tandem mass spectrometry with data-independent acquisition (DIA-PASEF). Bacterial DNA from subgingival biofilms was sequenced using the 16S rRNA gene for taxonomy assignment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Overall, 6147 bacterial and human proteins (≥ 2 peptides) were quantified. Despite the absence of attachment loss, the offspring of mothers with periodontitis presented with similar proteotypes as their mothers, characterised by up-regulation of inflammatory response cascades and down-regulation of epithelial barrier proteins. They also displayed higher colonisation patterns by periodontopathogens while presenting with increased expression of bacterial virulence proteins compared with controls.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The study showed that the maternal periodontal microbiome and proteome associate with those of the offspring and relate to maternal periodontal status. These early ecological events may potentially promote offspring's susceptibility to dysbiosis and may predispose them to periodontitis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 2","pages":"201-213"},"PeriodicalIF":6.8,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lee, S., J.-H. Chung, D. S Thoma, et al. “Bone Regeneration After Sinus Floor Elevation in an Intact Sinus or a Sinus With Prior Large Membrane Perforation: A Preclinical Study Using a Rabbit Sinus Model,” Journal of Clinical Periodontology 52, no. 3: 421–433. https://doi.org/10.1111/jcpe.14108.
Figure 4 is identical to Figure 3.
The title of Figure 4 is ‘Representative histological views of the sinus membrane in groups SFE_Perf and SFE’, but Figure 4 shows the entire histological views of groups SFE_Perf and SFE at 2 and 4 weeks.
We kindly request that the current Figure 4 be revised to reflect the correct version.
We apologise for this error.
李，S.， J.-H.。Chung， D. S . Thoma，等。“完整鼻窦或先前有大膜穿孔的鼻窦底抬高后骨再生：使用兔鼻窦模型的临床前研究”，《临床牙周病杂志》第52期。3: 421 - 433。https://doi.org/10.1111/jcpe.14108.Figure 4与图3相同。图4的标题是“SFE_Perf组和SFE组窦膜的代表性组织学视图”，但图4显示的是SFE_Perf组和SFE组在2周和4周时的整体组织学视图。我们恳请修改当前的图4以反映正确的版本。我们为这个错误道歉。
{"title":"Correction to ‘Bone Regeneration After Sinus Floor Elevation in an Intact Sinus or a Sinus With Prior Large Membrane Perforation: A Preclinical Study Using a Rabbit Sinus Model’","authors":"","doi":"10.1111/jcpe.70014","DOIUrl":"10.1111/jcpe.70014","url":null,"abstract":"<p>Lee, S., J.-H. Chung, D. S Thoma, et al. “Bone Regeneration After Sinus Floor Elevation in an Intact Sinus or a Sinus With Prior Large Membrane Perforation: A Preclinical Study Using a Rabbit Sinus Model,” <i>Journal of Clinical Periodontology</i> 52, no. 3: 421–433. https://doi.org/10.1111/jcpe.14108.</p><p>Figure 4 is identical to Figure 3.</p><p>The title of Figure 4 is ‘Representative histological views of the sinus membrane in groups SFE_Perf and SFE’, but Figure 4 shows the entire histological views of groups SFE_Perf and SFE at 2 and 4 weeks.</p><p>We kindly request that the current Figure 4 be revised to reflect the correct version.</p><p>We apologise for this error.</p>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"52 11","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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