Journal of Clinical Periodontology最新文献

英文中文

The Association of Periodontal Inflammation and Systemic Health Indicators: A Machine Learning Approach 牙周炎症与全身健康指标的关联：一种机器学习方法。

IF 6.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2025-07-23 DOI: 10.1111/jcpe.70000

Yumeng Yan, Praveen Sharma, Jeanie Suvan, Francesco D'Aiuto

Aim

The relationship between oral and systemic inflammation has profound implications for understanding the broader health impacts of periodontitis. The aim of this study was to (a) explore the association between periodontal inflammation and markers of systemic inflammation and metabolic health, and (b) preliminarily assess periodontal status based on systemic health indicators using machine learning techniques.

Methods

Data from a cross-sectional cohort (N = 667) were modelled (simple/multiple linear, fractional polynomial, logistic and random forest regression) to examine the association between systemic and periodontal measures. Three classifiers—random forest (RF), support vector machine (SVM) and gradient boosting (GB)—were used using periodontal inflamed surface area (PISA) and demographic and anthropometric variables (age, gender, ethnicity, body mass index [BMI] and smoking habits) as inputs to predict systemic inflammation (defined using serum C-reactive protein [CRP] levels). The best performing classification models (evaluated using area under the curve, AUC analyses) were validated using a second nationally representative dataset from the National Health and Nutrition Examination Surveys (NHANES) 2001–2002 and 2003–2004 combined datasets (N = 2288). Next, RF, SVM and GB were employed incorporating a set of systemic parameters (including serum CRP and lipid profiles) to predict the diagnosis of periodontitis. The best performing classification models were then validated using the NHANES 2009–2010 (N = 664) dataset.

Results

A nonlinear trend of CRP levels and PISA was confirmed by fractional polynomial regression (p = 0.008). Further, multiple linear regression analyses (adjusted for age, gender, ethnicity, BMI and smoking habits) confirmed a statistically significant relationship between log-transformed CRP levels and PISA (p < 0.0001). Logistic regression confirmed a relationship between PISA and low-density lipoprotein (LDL) in both crude and adjusted models. Among the classification models, SVM showed the highest performance in distinguishing CRP < 2 mg/L from CRP ≥ 2 mg/L (AUC = 0.71). The SVM model was successfully replicated in the NHANES 2001–2002 and 2003–2004 waves (AUC = 0.74). Prediction of periodontitis status (case vs. control) based on systemic indicators using the SVM model achieved the best performance with a mean AUC of 0.82. This was partially confirmed after external validation using the 2009–2010 NHANES dataset (AUC of 0.72).

目的口腔和全身炎症之间的关系对理解牙周炎对健康的广泛影响具有深远的意义。本研究的目的是(a)探索牙周炎症与全身炎症和代谢健康标志物之间的关系，以及(b)使用机器学习技术初步评估基于全身健康指标的牙周状态。方法对来自横断面队列（N = 667）的数据进行建模（简单/多元线性、分数多项式、逻辑回归和随机森林回归），以检验系统测量与牙周测量之间的关系。三种分类器——随机森林（RF）、支持向量机（SVM）和梯度增强（GB）——使用牙周发炎表面积（PISA）和人口统计学和人体测量变量（年龄、性别、种族、体重指数（BMI）和吸烟习惯）作为预测全身炎症（用血清c反应蛋白（CRP）水平定义）的输入。使用2001-2002年和2003-2004年国家健康和营养检查调查（NHANES）联合数据集（N = 2288）的第二个具有全国代表性的数据集验证了表现最佳的分类模型（使用曲线下面积和AUC分析进行评估）。然后，结合一组系统参数（包括血清CRP和脂质谱），采用RF、SVM和GB来预测牙周炎的诊断。然后使用NHANES 2009-2010 （N = 664）数据集对表现最佳的分类模型进行验证。结果分数多项式回归证实CRP水平与PISA的sa非线性趋势（p = 0.008）。此外，多元线性回归分析（调整了年龄、性别、种族、BMI和吸烟习惯）证实了对数转化CRP水平与PISA之间的统计学显著关系（p < 0.0001）。Logistic回归证实了PISA与低密度脂蛋白（LDL）之间的关系。在分类模型中，SVM对CRP < 2mg /L和CRP≥2mg /L的区分效果最好（AUC = 0.71）。支持向量机模型在2001-2002年和2003-2004年NHANES波浪中被成功复制（AUC = 0.74）。使用SVM模型基于系统指标预测牙周炎状态（病例与对照组）的效果最好，平均AUC为0.82。在使用2009-2010年NHANES数据集（AUC为0.72）进行外部验证后，部分证实了这一点。本研究通过机器学习模型证实了累积牙周炎症与全身炎症之间的一致关系。结合全身健康参数的预测模型有助于识别牙周炎病例。这两种模型都有可能用于初级卫生保健机构，包括筛查计划，因为它们证实了牙周炎和全身健康之间的双向联系。

{"title":"The Association of Periodontal Inflammation and Systemic Health Indicators: A Machine Learning Approach","authors":"Yumeng Yan, Praveen Sharma, Jeanie Suvan, Francesco D'Aiuto","doi":"10.1111/jcpe.70000","DOIUrl":"10.1111/jcpe.70000","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The relationship between oral and systemic inflammation has profound implications for understanding the broader health impacts of periodontitis. The aim of this study was to (a) explore the association between periodontal inflammation and markers of systemic inflammation and metabolic health, and (b) preliminarily assess periodontal status based on systemic health indicators using machine learning techniques.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Data from a cross-sectional cohort (<i>N</i> = 667) were modelled (simple/multiple linear, fractional polynomial, logistic and random forest regression) to examine the association between systemic and periodontal measures. Three classifiers—random forest (RF), support vector machine (SVM) and gradient boosting (GB)—were used using periodontal inflamed surface area (PISA) and demographic and anthropometric variables (age, gender, ethnicity, body mass index [BMI] and smoking habits) as inputs to predict systemic inflammation (defined using serum C-reactive protein [CRP] levels). The best performing classification models (evaluated using area under the curve, AUC analyses) were validated using a second nationally representative dataset from the National Health and Nutrition Examination Surveys (NHANES) 2001–2002 and 2003–2004 combined datasets (<i>N</i> = 2288). Next, RF, SVM and GB were employed incorporating a set of systemic parameters (including serum CRP and lipid profiles) to predict the diagnosis of periodontitis. The best performing classification models were then validated using the NHANES 2009–2010 (<i>N</i> = 664) dataset.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A nonlinear trend of CRP levels and PISA was confirmed by fractional polynomial regression (<i>p</i> = 0.008). Further, multiple linear regression analyses (adjusted for age, gender, ethnicity, BMI and smoking habits) confirmed a statistically significant relationship between log-transformed CRP levels and PISA (<i>p</i> < 0.0001). Logistic regression confirmed a relationship between PISA and low-density lipoprotein (LDL) in both crude and adjusted models. Among the classification models, SVM showed the highest performance in distinguishing CRP < 2 mg/L from CRP ≥ 2 mg/L (AUC = 0.71). The SVM model was successfully replicated in the NHANES 2001–2002 and 2003–2004 waves (AUC = 0.74). Prediction of periodontitis status (case vs. control) based on systemic indicators using the SVM model achieved the best performance with a mean AUC of 0.82. This was partially confirmed after external validation using the 2009–2010 NHANES dataset (AUC of 0.72).</p>\u0000 </section>\u0000 \u0000 ","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"52 10","pages":"1466-1477"},"PeriodicalIF":6.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70000","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early Placement of Patient-Specific One-Piece Drill-Free Fully Digital Designed Dental Implants: A Feasibility Study 患者特异性单件无钻全数字化设计牙种植体的早期植入：可行性研究。

IF 6.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2025-07-20 DOI: 10.1111/jcpe.14204

Giuseppe Alexandre Romito, Mohamed Ahmed Hassan, Marina Nogueira de Castro Galvão Corrente, Julia Dahdal Aoun, Vitor Marques Sapata, Roger Nishyama, Cristina Cunha Villar

Aim

CBCT and CAD/CAM technologies have allowed the development of patient-specific implants requiring no drilling. This prospective study evaluated the safety and effectiveness of a novel, drill-free, one-piece root-analogue implant designed using a digital workflow and manufactured additively.

Materials and Methods

Patients with non-restorable maxillary anterior or premolar teeth requiring extraction and implant placement were included. Implant design was based on pre-extraction cone-beam computed tomography (CBCT) and optical impressions of the target tooth, adjacent teeth and opposing dentition, and post-extraction scanning of the extracted tooth. Within 14 days post extraction, the implant was designed, manufactured and placed into the alveolar socket, and a temporary crown was installed out of occlusion. The final crown was installed after 3 months. Clinical parameters, including plaque index, gingival index, bleeding on probing, suppuration, peri-implant mucosal margin position, probing depth, probing depth relative to the implant platform and keratinised tissue, were collected at 3, 6, 9, 12 and 24 months post loading. Radiographic measurements as well as patient- and clinician-reported outcomes were also assessed.

Results

The study cohort comprised 12 patients. The cumulative implant survival and success rates reached 100% and 90%, respectively. Clinical and radiographic parameters consistently indicated healthy peri-implant tissues. Patient-reported outcomes demonstrated high satisfaction and minimal discomfort. Visual analogue scale (VAS) scores for overall satisfaction remained high, with a median of 10 at final restoration delivery (95% CI: 10.00–10.00) and at the 24-month follow-up (95% CI: 9.70–10.01).

Conclusion

These patient-specific, root-analogue implants demonstrated both safety and effectiveness, along with high patient satisfaction rates up to 24 months post loading.

AIMCBCT和CAD/CAM技术使得无需钻孔的患者特异性植入物得以开发。这项前瞻性研究评估了一种新型、无钻孔、一体式根模拟种植体的安全性和有效性，该种植体采用数字工作流程设计，并采用增材制造。材料与方法包括上颌前磨牙或前磨牙不可修复，需要拔牙和种植体的患者。种植体设计基于拔牙前的锥形束计算机断层扫描（CBCT）和目标牙齿、相邻牙齿和对牙列的光学印象，以及拔牙后对拔牙的扫描。拔牙后14天内，设计、制作种植体并将其放置在牙槽窝中，并安装临时冠以避免咬合。3个月后安装了最终的冠。于加载后3、6、9、12和24个月收集临床参数，包括菌斑指数、牙龈指数、探诊出血、化脓、种植体周围粘膜边缘位置、探诊深度、相对于种植体平台和角化组织的探诊深度。放射测量以及患者和临床报告的结果也进行了评估。结果研究队列包括12例患者。累积种植体成活率为100%，成功率为90%。临床和影像学参数一致显示种植体周围组织健康。患者报告的结果显示高满意度和最小的不适。视觉模拟量表（VAS）总体满意度评分仍然很高，在最终修复交付时（95% CI: 10.00-10.00）和24个月随访时（95% CI: 9.70-10.01）的中位数为10。结论：这些患者特异性的根模拟种植体具有安全性和有效性，并且在植入后24个月患者满意度较高。

{"title":"Early Placement of Patient-Specific One-Piece Drill-Free Fully Digital Designed Dental Implants: A Feasibility Study","authors":"Giuseppe Alexandre Romito, Mohamed Ahmed Hassan, Marina Nogueira de Castro Galvão Corrente, Julia Dahdal Aoun, Vitor Marques Sapata, Roger Nishyama, Cristina Cunha Villar","doi":"10.1111/jcpe.14204","DOIUrl":"10.1111/jcpe.14204","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>CBCT and CAD/CAM technologies have allowed the development of patient-specific implants requiring no drilling. This prospective study evaluated the safety and effectiveness of a novel, drill-free, one-piece root-analogue implant designed using a digital workflow and manufactured additively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Patients with non-restorable maxillary anterior or premolar teeth requiring extraction and implant placement were included. Implant design was based on pre-extraction cone-beam computed tomography (CBCT) and optical impressions of the target tooth, adjacent teeth and opposing dentition, and post-extraction scanning of the extracted tooth. Within 14 days post extraction, the implant was designed, manufactured and placed into the alveolar socket, and a temporary crown was installed out of occlusion. The final crown was installed after 3 months. Clinical parameters, including plaque index, gingival index, bleeding on probing, suppuration, peri-implant mucosal margin position, probing depth, probing depth relative to the implant platform and keratinised tissue, were collected at 3, 6, 9, 12 and 24 months post loading. Radiographic measurements as well as patient- and clinician-reported outcomes were also assessed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The study cohort comprised 12 patients. The cumulative implant survival and success rates reached 100% and 90%, respectively. Clinical and radiographic parameters consistently indicated healthy peri-implant tissues. Patient-reported outcomes demonstrated high satisfaction and minimal discomfort. Visual analogue scale (VAS) scores for overall satisfaction remained high, with a median of 10 at final restoration delivery (95% CI: 10.00–10.00) and at the 24-month follow-up (95% CI: 9.70–10.01).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These patient-specific, root-analogue implants demonstrated both safety and effectiveness, along with high patient satisfaction rates up to 24 months post loading.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"52 10","pages":"1374-1385"},"PeriodicalIF":6.8,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.14204","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Surgical Periodontal Therapy Modulates Oral Microbiome in Primary Immunodeficient Children 非手术牙周治疗调节原发性免疫缺陷儿童的口腔微生物组。

IF 6.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2025-07-20 DOI: 10.1111/jcpe.14201

Abish S. Stephen, Stacy Worrall, Cheryl Somani, Robert P. Allaker, Janet Davies, Luigi Nibali, Nikolaos Donos

Background and Aim

Primary Immunodeficiencies (PIDs) arise from rare genetic defects affecting humoral and cellular immunity, which can lead to reduced dental plaque control. This study aimed to characterise the subgingival dental plaque microbiome in neutropenic PID children compared to healthy controls and assess their response to non-surgical periodontal therapy.

Methods

Subgingival plaque was collected from three first molars and one first incisor at baseline and 6 months post therapy from children with PID (n = 24) and systematically healthy control participants (n = 24) who were recruited from Great Ormond Street Hospital and Barts Health NHS Trust, respectively. The subgingival microbiome was profiled using an Illumina metabarcoding approach on the bacterial 16S rRNA gene V1–V2 region.

Results

Significant shifts in community structure were observed post therapy, as measured by alpha and beta diversities. An increase in Rothia spp., Neisseria spp. and Actinomyces spp. was noted in PID children post therapy, consistent with clinical improvements. Baseline blood absolute neutrophil counts in PID children were positively associated with Streptococcus cristatus and Gemella spp., and negatively with Saccharibacteria, Capnocytophaga and Porphyromonas spp., highlighting key host–microbial relationships.

Conclusion

Non-surgical periodontal therapy modulated the subgingival microbiota in neutropenic PID children, revealing novel host–microbial interactions important for the oral microbiome in health.

原发性免疫缺陷（pid）是由影响体液和细胞免疫的罕见遗传缺陷引起的，这可能导致对牙菌斑的控制减少。本研究旨在描述中性粒细胞减少的PID儿童与健康对照组相比龈下牙菌斑微生物组的特征，并评估他们对非手术牙周治疗的反应。方法分别从大奥蒙德街医院（Great Ormond Street Hospital）和巴茨健康NHS信托基金（Barts Health NHS Trust）招募的患有PID的儿童（n = 24）和系统健康对照组（n = 24），在基线和治疗后6个月分别从3颗第一磨牙和1颗第一门牙上收集牙龈下菌斑。采用Illumina元条形码方法对细菌16S rRNA基因V1-V2区域进行龈下微生物组分析。结果通过α和β多样性测量，观察到治疗后社区结构的显著变化。在治疗后的PID患儿中，罗氏菌属、奈瑟菌属和放线菌属的数量有所增加，与临床改善一致。PID患儿的基线血液绝对中性粒细胞计数与criptostatus和Gemella spp呈正相关，与saccharibacterium， Capnocytophaga和Porphyromonas spp呈负相关，突出了关键的宿主-微生物关系。结论：非手术牙周治疗可调节中性粒细胞减少的PID儿童的龈下微生物群，揭示了新的宿主-微生物相互作用对健康口腔微生物群的重要作用。

{"title":"Non-Surgical Periodontal Therapy Modulates Oral Microbiome in Primary Immunodeficient Children","authors":"Abish S. Stephen, Stacy Worrall, Cheryl Somani, Robert P. Allaker, Janet Davies, Luigi Nibali, Nikolaos Donos","doi":"10.1111/jcpe.14201","DOIUrl":"10.1111/jcpe.14201","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Aim</h3>\u0000 \u0000 <p>Primary Immunodeficiencies (PIDs) arise from rare genetic defects affecting humoral and cellular immunity, which can lead to reduced dental plaque control. This study aimed to characterise the subgingival dental plaque microbiome in neutropenic PID children compared to healthy controls and assess their response to non-surgical periodontal therapy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Subgingival plaque was collected from three first molars and one first incisor at baseline and 6 months post therapy from children with PID (<i>n</i> = 24) and systematically healthy control participants (<i>n</i> = 24) who were recruited from Great Ormond Street Hospital and Barts Health NHS Trust, respectively. The subgingival microbiome was profiled using an Illumina metabarcoding approach on the bacterial 16S rRNA gene V1–V2 region.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant shifts in community structure were observed post therapy, as measured by alpha and beta diversities. An increase in <i>Rothia</i> spp., <i>Neisseria</i> spp. and <i>Actinomyces</i> spp. was noted in PID children post therapy, consistent with clinical improvements. Baseline blood absolute neutrophil counts in PID children were positively associated with \u0000 <i>Streptococcus cristatus</i>\u0000 and <i>Gemella</i> spp., and negatively with <i>Saccharibacteria, Capnocytophaga</i> and <i>Porphyromonas</i> spp., highlighting key host–microbial relationships.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Non-surgical periodontal therapy modulated the subgingival microbiota in neutropenic PID children, revealing novel host–microbial interactions important for the oral microbiome in health.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"52 10","pages":"1410-1418"},"PeriodicalIF":6.8,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.14201","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Loci Associated With Periodontitis: The FinnGen Study Based on National Health Registers 牙周炎相关基因位点：基于国家健康登记的FinnGen研究

IF 6.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2025-07-19 DOI: 10.1111/jcpe.14193

Aino Salminen, Kati Hyvärinen, Jarmo Ritari, Ana Caetano, Oleg Kambur, Päivi Mäntylä, Mustafa Yilmaz, Juha Sinisalo, FinnGen, Markus Perola, Aki Havulinna, Luigi Nibali, Ulvi Kahraman Gürsoy, Pirkko J. Pussinen

Aim

To perform a genome-wide association study (GWAS) for periodontitis in the FinnGen cohort, as genetic factors contribute to periodontitis.

Materials and Methods

We included nearly 250,000 Finnish individuals who had visited a dentist in the public healthcare sector for a clinical oral examination. We designed three periodontitis phenotypes based on diagnosis and procedure codes and CPI indexes in national health registers.

Results

We identified 11 independent genetic loci associated with periodontitis, among which 6 were common and novel. A locus near the FST gene was associated with two phenotypes, whereas other lead SNPs were located near ARL15, MFHAS1, DEFB130A and APOE. Additionally, all phenotypes in the discovery and replication cohorts were associated with genetic variations in the HLA region. Furthermore, imputed HLA allele frequencies identified independent associations between HLA-DRB1, HLA-DPB1 and HLA-DQA1 and periodontitis. Based on single-cell RNA sequencing, the expression of genes near our lead SNPs across all three phenotypes was particularly enriched in gingival cell lineages important in the pathogenesis of periodontitis. Phenotypical and genetic correlations revealed associations between periodontitis and bacterial diseases, as well as autoimmune and cardiometabolic phenotypes.

Conclusions

Our GWAS suggests that genetic variation contributing to immune dysregulation is involved in the pathogenesis of periodontitis, which has considerable genetic similarity with other complex traits.

目的：在FinnGen队列中对牙周炎进行全基因组关联研究（GWAS），因为遗传因素有助于牙周炎。材料和方法我们纳入了近25万名在公共卫生部门就诊牙医进行临床口腔检查的芬兰人。我们设计了三种牙周炎表型根据诊断和程序代码和CPI指数在国家卫生登记。结果鉴定出11个与牙周炎相关的独立基因位点，其中6个为常见的新基因位点。FST基因附近的位点与两种表型相关，而其他先导snp位于ARL15、MFHAS1、DEFB130A和APOE附近。此外，发现和复制队列中的所有表型都与HLA区域的遗传变异有关。此外，输入的HLA等位基因频率确定了HLA- drb1、HLA- dpb1和HLA- dqa1与牙周炎之间的独立关联。基于单细胞RNA测序，在所有三种表型中，我们的主要snp附近的基因表达在牙周炎发病机制中重要的牙龈细胞系中特别丰富。表型和遗传相关性揭示了牙周炎与细菌性疾病以及自身免疫和心脏代谢表型之间的关联。结论GWAS提示导致免疫失调的遗传变异参与了牙周炎的发病机制，与其他复杂性状具有相当的遗传相似性。

{"title":"Genetic Loci Associated With Periodontitis: The FinnGen Study Based on National Health Registers","authors":"Aino Salminen, Kati Hyvärinen, Jarmo Ritari, Ana Caetano, Oleg Kambur, Päivi Mäntylä, Mustafa Yilmaz, Juha Sinisalo, FinnGen, Markus Perola, Aki Havulinna, Luigi Nibali, Ulvi Kahraman Gürsoy, Pirkko J. Pussinen","doi":"10.1111/jcpe.14193","DOIUrl":"10.1111/jcpe.14193","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To perform a genome-wide association study (GWAS) for periodontitis in the FinnGen cohort, as genetic factors contribute to periodontitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>We included nearly 250,000 Finnish individuals who had visited a dentist in the public healthcare sector for a clinical oral examination. We designed three periodontitis phenotypes based on diagnosis and procedure codes and CPI indexes in national health registers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We identified 11 independent genetic loci associated with periodontitis, among which 6 were common and novel. A locus near the <i>FST</i> gene was associated with two phenotypes, whereas other lead SNPs were located near <i>ARL15</i>, <i>MFHAS1</i>, <i>DEFB130A</i> and <i>APOE</i>. Additionally, all phenotypes in the discovery and replication cohorts were associated with genetic variations in the HLA region. Furthermore, imputed HLA allele frequencies identified independent associations between <i>HLA-DRB1</i>, <i>HLA-DPB1</i> and <i>HLA-DQA1</i> and periodontitis. Based on single-cell RNA sequencing, the expression of genes near our lead SNPs across all three phenotypes was particularly enriched in gingival cell lineages important in the pathogenesis of periodontitis. Phenotypical and genetic correlations revealed associations between periodontitis and bacterial diseases, as well as autoimmune and cardiometabolic phenotypes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our GWAS suggests that genetic variation contributing to immune dysregulation is involved in the pathogenesis of periodontitis, which has considerable genetic similarity with other complex traits.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"52 9","pages":"1263-1275"},"PeriodicalIF":6.8,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.14193","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144664263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Periodontal Benefits of Alveolar Ridge Augmentation With Xenograft Following Third Molar Extraction: A Randomised Controlled Trial 第三磨牙拔除后异种移植牙槽嵴增强对牙周的益处：一项随机对照试验。

IF 6.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2025-07-13 DOI: 10.1111/jcpe.14205

Su Sheng Quach, Nigel Johnson, Raahib Dudhia, Anthony Puljich, Sašo Ivanovski, Ryan S. B. Lee

Aim

To evaluate the effect of deproteinised bovine bone mineral with collagen (DBBM-C) grafting on periodontal healing at the distal aspect of the second molar (D2M) during adjacent third molar extraction.

Material and Methods

Forty-two sites in 28 patients were randomly allocated into two groups. The test group (18 sites) received DBBM-C grafting during extraction, whereas the control group (24 sites) received extraction alone. The patients were reviewed 6 months postoperatively, and the D2M and extraction sites were evaluated by both linear and volumetric cone beam computed tomography measurements.

Results

Nine patients dropped out (14 sites) and two patients were excluded (4 sites), leaving a total of 17 patients (24 sites) who were reviewed after 6 months. Both groups showed significant healing when examining the linear measurements (except at the disto-lingual aspect of the second molar) and the defined volumes of interest. There were, however, no significant differences between the groups in the linear or volumetric outcome analyses.

Conclusions

Within the limitations of the study, the use of DBBM-C during third molar extraction did not improve periodontal healing at the D2M. This trial was registered in April 2020 in the Australian New Zealand Clinical Trials Registry under code ACTRN12620000497909 (https://anzctr.org.au/Trial/Registration/TrialReview.aspx?ACTRN=12620000497909).

目的探讨脱蛋白牛骨矿胶原（DBBM-C）移植对相邻第三磨牙拔牙时第二磨牙远端牙周愈合的影响。材料与方法28例患者的42个部位随机分为两组。实验组（18个位点）在拔牙过程中进行DBBM-C接枝，对照组（24个位点）单独拔牙。术后6个月复查患者，通过线性和体积锥形束计算机断层扫描测量评估D2M和拔牙部位。结果9例患者退出（14个点），2例患者被排除（4个点），6个月后复查17例患者（24个点）。当检查线性测量（除了第二磨牙的离舌侧面）和确定的兴趣体积时，两组都显示出显著的愈合。然而，在线性或容积结果分析中，两组之间没有显著差异。结论：在本研究的限制范围内，在第三磨牙拔牙时使用DBBM-C并没有改善D2M的牙周愈合。该试验于2020年4月在澳大利亚新西兰临床试验注册中心注册，代码为ACTRN12620000497909 （https://anzctr.org.au/Trial/Registration/TrialReview.aspx?ACTRN=12620000497909）。

{"title":"The Periodontal Benefits of Alveolar Ridge Augmentation With Xenograft Following Third Molar Extraction: A Randomised Controlled Trial","authors":"Su Sheng Quach, Nigel Johnson, Raahib Dudhia, Anthony Puljich, Sašo Ivanovski, Ryan S. B. Lee","doi":"10.1111/jcpe.14205","DOIUrl":"10.1111/jcpe.14205","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To evaluate the effect of deproteinised bovine bone mineral with collagen (DBBM-C) grafting on periodontal healing at the distal aspect of the second molar (D2M) during adjacent third molar extraction.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Forty-two sites in 28 patients were randomly allocated into two groups. The test group (18 sites) received DBBM-C grafting during extraction, whereas the control group (24 sites) received extraction alone. The patients were reviewed 6 months postoperatively, and the D2M and extraction sites were evaluated by both linear and volumetric cone beam computed tomography measurements.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Nine patients dropped out (14 sites) and two patients were excluded (4 sites), leaving a total of 17 patients (24 sites) who were reviewed after 6 months. Both groups showed significant healing when examining the linear measurements (except at the disto-lingual aspect of the second molar) and the defined volumes of interest. There were, however, no significant differences between the groups in the linear or volumetric outcome analyses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Within the limitations of the study, the use of DBBM-C during third molar extraction did not improve periodontal healing at the D2M. This trial was registered in April 2020 in the Australian New Zealand Clinical Trials Registry under code ACTRN12620000497909 (https://anzctr.org.au/Trial/Registration/TrialReview.aspx?ACTRN=12620000497909).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"52 10","pages":"1419-1429"},"PeriodicalIF":6.8,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.14205","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144622174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability of Augmented Bone and Its Influencing Factors After Simultaneous Guided Bone Regeneration With Implant Placement in the Posterior Mandible: A Retrospective Study 后下颌骨同时引导骨再生与种植体植入后增强骨的稳定性及其影响因素：回顾性研究

IF 6.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2025-07-10 DOI: 10.1111/jcpe.14206

Xiaoyu Chen, Wenting Qi, Siyuan Wang, Antian Xu, Hongye Lu, Xiaoting Shen, Fuming He

Aim

To analyse the stability of augmented bone and its influencing factors after simultaneous guided bone regeneration (GBR) with implant placement in the posterior mandible.

Materials and Methods

A total of 165 implants in 102 patients were included. General information, peri-implant soft-tissue parameters and complications were recorded. Cone-beam computed tomography images at preoperative (T0), immediate postoperative (T1), post-healing (T2) and the latest follow-up (T3) were collected. Buccal bone width, height, bone distance (BD) and augmented bone volume (ABV) were assessed. Bone augmentation range was classified into inside-contour (IC) group and outside-contour (OC) group based on BD values. Factors influencing the augmented bone volume resorption rate (ABV%) were analysed.

Results

During the follow-up period of 12–88 months, the mean ABV% was 47.56% ± 20.29%, predominantly occurring between T1 and T2. The OC group showed higher ABV% compared to the IC group (p < 0.001). BD of the IC and 0–1 mm OC groups was less than 0, while BD of the 1–2 and > 2 mm OC groups was near 0 at T3. Bone augmentation range (p < 0.001), non-contained defects (p = 0.001) and 2-mm healing abutments (p = 0.008) significantly influenced ABV%.

Conclusions

Simultaneous GBR with implant placement in the posterior mandible provided predictable volumetric stability of the augmented bone. OC grafts resorbed towards individual phenotypical dimensions, whereas 1–2 mm over-augmentation might optimise contour maintenance. Non-contained defects compromised volumetric stability, while the 2-mm healing abutments enhanced stability compared to cover screws.

目的分析后颌骨引导骨再生（GBR）联合种植体植入后增强骨的稳定性及其影响因素。材料与方法102例患者共165颗种植体。记录一般信息、种植体周围软组织参数和并发症。收集术前（T0）、术后即刻（T1）、愈合后（T2）和最新随访（T3）的锥形束计算机断层扫描图像。评估颊骨宽度、高度、骨距（BD）和增强骨体积（ABV）。根据BD值将骨增强范围分为内轮廓（IC）组和外轮廓（OC）组。分析影响增强骨体积吸收率的因素。结果随访12 ~ 88个月，平均ABV%为47.56%±20.29%，主要发生在T1和T2之间。与IC组相比，OC组ABV%较高(p <；0.001)。IC组和0 ~ 1 mm OC组的BD < 0, 1 ~ 2 mm OC组和1 ~ 2 mm OC组的BD < 0；2 mm OC组在T3时接近0。骨增强范围(p <；0.001)，不含缺陷（p = 0.001）和2mm愈合基台（p = 0.008）显著影响ABV%。结论GBR与种植体同时植入后颌骨可提供可预测的增强骨体积稳定性。OC移植物向个体表型尺寸吸收，而1-2毫米的过度增大可能会优化轮廓维持。非包含缺陷损害了体积稳定性，而2毫米愈合基台与盖螺钉相比增强了稳定性。

{"title":"Stability of Augmented Bone and Its Influencing Factors After Simultaneous Guided Bone Regeneration With Implant Placement in the Posterior Mandible: A Retrospective Study","authors":"Xiaoyu Chen, Wenting Qi, Siyuan Wang, Antian Xu, Hongye Lu, Xiaoting Shen, Fuming He","doi":"10.1111/jcpe.14206","DOIUrl":"10.1111/jcpe.14206","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To analyse the stability of augmented bone and its influencing factors after simultaneous guided bone regeneration (GBR) with implant placement in the posterior mandible.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A total of 165 implants in 102 patients were included. General information, peri-implant soft-tissue parameters and complications were recorded. Cone-beam computed tomography images at preoperative (T0), immediate postoperative (T1), post-healing (T2) and the latest follow-up (T3) were collected. Buccal bone width, height, bone distance (BD) and augmented bone volume (ABV) were assessed. Bone augmentation range was classified into inside-contour (IC) group and outside-contour (OC) group based on BD values. Factors influencing the augmented bone volume resorption rate (ABV%) were analysed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>During the follow-up period of 12–88 months, the mean ABV% was 47.56% ± 20.29%, predominantly occurring between T1 and T2. The OC group showed higher ABV% compared to the IC group (<i>p</i> < 0.001). BD of the IC and 0–1 mm OC groups was less than 0, while BD of the 1–2 and > 2 mm OC groups was near 0 at T3. Bone augmentation range (<i>p</i> < 0.001), non-contained defects (<i>p</i> = 0.001) and 2-mm healing abutments (<i>p</i> = 0.008) significantly influenced ABV%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Simultaneous GBR with implant placement in the posterior mandible provided predictable volumetric stability of the augmented bone. OC grafts resorbed towards individual phenotypical dimensions, whereas 1–2 mm over-augmentation might optimise contour maintenance. Non-contained defects compromised volumetric stability, while the 2-mm healing abutments enhanced stability compared to cover screws.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"52 10","pages":"1430-1441"},"PeriodicalIF":6.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144603083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Periodontal Condition With Serum C-Reactive Protein Levels: The Role of Serum Apolipoproteins' Concentration 牙周状况与血清C反应蛋白水平的关系：血清载脂蛋白浓度的作用

IF 6.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2025-07-10 DOI: 10.1111/jcpe.14202

Anniina Haro, Tuomas Saxlin, Anna Liisa Suominen, Antti Jula, Pekka Ylöstalo

Aim

To investigate whether the systemic inflammatory response against inflammatory conditions in the periodontium is related to serum apolipoprotein B (ApoB) and A1 (ApoA1) concentrations.

Material and Methods

The study consisted of the Health 2000 Survey participants (n = 2709) aged 30–49 years. The inflammatory condition of the periodontium was assessed by means of the number of teeth with deepened (≥ 4 mm) and deep (≥ 6 mm) periodontal pockets. Systemic inflammation was measured by serum C-reactive protein (CRP) levels. The role of ApoB and ApoA1 was studied by performing regression analyses and stratified analyses (according to the median values).

Results

In logistic regression analyses, the number of teeth with deepened (≥ 4 mm) periodontal pockets was associated with serum CRP levels among those participants whose serum ApoB concentration was ≥ 1.12 g/L. When the participants' ApoB concentration was < 1.12 g/L, such an association between deepened periodontal pockets and CRP was not observed. The direction or strength of the association between periodontal pockets and CRP was not essentially different in the ApoA1 strata.

Conclusion

Systemic response against poor periodontal condition varied between individuals. The variation appeared to be related more to the serum concentration of ApoB than ApoA1.

目的探讨血清载脂蛋白B （ApoB）和A1 （ApoA1）浓度是否与牙周组织炎症反应有关。材料与方法本研究纳入健康2000调查参与者（n = 2709），年龄30-49岁。通过深度（≥4mm）和深度（≥6mm）牙周袋的牙数来评估牙周组织的炎症情况。通过血清C反应蛋白（CRP）水平检测全身性炎症。通过回归分析和分层分析（按中位数）研究ApoB和ApoA1的作用。结果经logistic回归分析，血清载脂蛋白（ApoB）浓度≥1.12 g/L的受试者牙周袋深度（≥4 mm）牙数与血清CRP水平相关。当参与者的ApoB浓度为<；1.12 g/L时，未观察到牙周袋加深与CRP之间的相关性。在ApoA1层中，牙周袋与CRP之间的关联方向或强度并无本质差异。结论不同个体对牙周不良的全身反应存在差异。这种变异似乎与血清ApoB浓度比ApoA1浓度更相关。

{"title":"Association of Periodontal Condition With Serum C-Reactive Protein Levels: The Role of Serum Apolipoproteins' Concentration","authors":"Anniina Haro, Tuomas Saxlin, Anna Liisa Suominen, Antti Jula, Pekka Ylöstalo","doi":"10.1111/jcpe.14202","DOIUrl":"10.1111/jcpe.14202","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To investigate whether the systemic inflammatory response against inflammatory conditions in the periodontium is related to serum apolipoprotein B (ApoB) and A1 (ApoA1) concentrations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>The study consisted of the Health 2000 Survey participants (<i>n</i> = 2709) aged 30–49 years. The inflammatory condition of the periodontium was assessed by means of the number of teeth with deepened (≥ 4 mm) and deep (≥ 6 mm) periodontal pockets. Systemic inflammation was measured by serum C-reactive protein (CRP) levels. The role of ApoB and ApoA1 was studied by performing regression analyses and stratified analyses (according to the median values).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In logistic regression analyses, the number of teeth with deepened (≥ 4 mm) periodontal pockets was associated with serum CRP levels among those participants whose serum ApoB concentration was ≥ 1.12 g/L. When the participants' ApoB concentration was < 1.12 g/L, such an association between deepened periodontal pockets and CRP was not observed. The direction or strength of the association between periodontal pockets and CRP was not essentially different in the ApoA1 strata.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Systemic response against poor periodontal condition varied between individuals. The variation appeared to be related more to the serum concentration of ApoB than ApoA1.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"52 10","pages":"1454-1465"},"PeriodicalIF":6.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.14202","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144603085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KAT6A Promotes Macrophage Inflammation and Periodontitis KAT6A促进巨噬细胞炎症和牙周炎

IF 6.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2025-07-09 DOI: 10.1111/jcpe.14203

Shi-Jia Huang, Shuo Xu, Hui-Lin Ye, Lu-Jun Zhou, Yong-Li Wang, Sheng-Zhong Duan

Both epigenetic modification and immunoregulation play important roles in periodontitis (PD). However, the function of macrophage lysine acetyltransferase 6A (KAT6A) in PD remains unknown. In this study, we first analysed single-cell RNA sequencing data and demonstrated up-regulated KAT6A expression in periodontal tissue macrophages of PD patients. Subsequently, macrophage KAT6A knockout (MK6AKO) mice were generated and subjected to PD induction by a combination of molar ligation and application of Porphyromonas gingivalis (Pg). Morphological analyses showed significantly decreased alveolar bone resorption in MK6AKO mice, and qRT-PCR analysis showed markedly attenuated expression of inflammatory genes in the gingiva of MK6AKO mice compared to littermate mice after PD induction. RNA-seq of peritoneal macrophages stimulated with Pg lipopolysaccharides (Pg-LPS) unveiled down-regulation of inflammatory pathways, particularly the interleukin 17 pathway in MK6AKO macrophages. Flow cytometry analysis detected a notable reduction of neutrophils in the gingiva of MK6AKO mice after PD induction. Furthermore, using the Cut&tag technique, we identified reduced histone H3K27 acetylation levels at nuclear factor kappa-B binding sites on promoters of interleukin 1β and tumour necrosis factor in MK6AKO macrophages treated with Pg-LPS. In summary, our study highlights the significant role of KAT6A in modulating macrophage phenotypes and the progression of PD, suggesting the therapeutic potential of targeting KAT6A.

表观遗传修饰和免疫调节在牙周炎（PD）中起重要作用。然而，巨噬细胞赖氨酸乙酰转移酶6A （KAT6A）在PD中的功能尚不清楚。在这项研究中，我们首先分析了单细胞RNA测序数据，并证明了PD患者牙周组织巨噬细胞中KAT6A表达上调。随后，产生巨噬细胞KAT6A敲除（MK6AKO）小鼠，并通过磨牙结扎和应用牙龈卟啉单胞菌（Pg）联合诱导PD。形态学分析显示，MK6AKO小鼠的牙槽骨吸收明显减少，qRT - PCR分析显示，与同窝小鼠相比，PD诱导后MK6AKO小鼠的牙龈炎症基因表达明显减弱。用Pg脂多糖（Pg‐LPS）刺激的腹膜巨噬细胞的RNA‐seq揭示了炎症通路的下调，特别是MK6AKO巨噬细胞的白细胞介素17通路。流式细胞术分析发现，PD诱导后MK6AKO小鼠牙龈中性粒细胞明显减少。此外，使用Cut&；tag技术，我们发现Pg‐LPS处理的MK6AKO巨噬细胞中，白细胞介素1β和肿瘤坏死因子启动子上核因子kappa‐B结合位点的组蛋白H3K27乙酰化水平降低。综上所述，我们的研究强调了KAT6A在调节巨噬细胞表型和PD进展中的重要作用，提示靶向KAT6A的治疗潜力。

{"title":"KAT6A Promotes Macrophage Inflammation and Periodontitis","authors":"Shi-Jia Huang, Shuo Xu, Hui-Lin Ye, Lu-Jun Zhou, Yong-Li Wang, Sheng-Zhong Duan","doi":"10.1111/jcpe.14203","DOIUrl":"10.1111/jcpe.14203","url":null,"abstract":"<div>\u0000 \u0000 <p>Both epigenetic modification and immunoregulation play important roles in periodontitis (PD). However, the function of macrophage lysine acetyltransferase 6A (KAT6A) in PD remains unknown. In this study, we first analysed single-cell RNA sequencing data and demonstrated up-regulated KAT6A expression in periodontal tissue macrophages of PD patients. Subsequently, macrophage KAT6A knockout (MK6AKO) mice were generated and subjected to PD induction by a combination of molar ligation and application of \u0000 <i>Porphyromonas gingivalis</i>\u0000 (<i>Pg</i>). Morphological analyses showed significantly decreased alveolar bone resorption in MK6AKO mice, and qRT-PCR analysis showed markedly attenuated expression of inflammatory genes in the gingiva of MK6AKO mice compared to littermate mice after PD induction. RNA-seq of peritoneal macrophages stimulated with <i>Pg</i> lipopolysaccharides (<i>Pg</i>-LPS) unveiled down-regulation of inflammatory pathways, particularly the interleukin 17 pathway in MK6AKO macrophages. Flow cytometry analysis detected a notable reduction of neutrophils in the gingiva of MK6AKO mice after PD induction. Furthermore, using the Cut&tag technique, we identified reduced histone H3K27 acetylation levels at nuclear factor kappa-B binding sites on promoters of interleukin 1β and tumour necrosis factor in MK6AKO macrophages treated with <i>Pg</i>-LPS. In summary, our study highlights the significant role of KAT6A in modulating macrophage phenotypes and the progression of PD, suggesting the therapeutic potential of targeting KAT6A.</p>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"52 10","pages":"1499-1508"},"PeriodicalIF":6.8,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144593896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Treatment Endpoints After Active Periodontal Treatment and 10 Years of Supportive Periodontal Care: A Retrospective Cohort Study 积极牙周治疗和10年支持牙周护理后的临床治疗终点：一项回顾性队列研究

IF 6.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2025-07-08 DOI: 10.1111/jcpe.14179

Mario Schröder, Max Buchinger, Iulia Dahmer, Peter Eickholz, Hari Petsos

Objectives

Comparing periodontal stability following active periodontal treatment (APT/T1) and 120 ± 12 months of supportive periodontal care (SPC/T2) using four clinical endpoints (CEPs).

Methods

CEP1: pocket probing depths (PPD) ≤ 4 mm, no sites with ≥ 4 mm with bleeding on probing (BOP), and total BOP < 10%; CEP2: no PPD > 4 mm with BOP or PPD ≥ 6 mm; CEP3: ≤ 4 sites with PPD ≥ 5 mm; CEP4: ≤ 5 teeth with PPD ≥ 5 mm. Assuming CEPs are mutually exclusive, patient- and tooth-related parameters (e.g., periodontal tooth loss: PTL) were compared. Using receiver operating characteristic analysis for the prediction of PTL as a cutoff for CEP was assessed.

Results

From 128 patients (age 65.5 ± 10.5 years; 83 stage III, 45 stage IV; 47 grade B, 81 grade C), 7 achieved CEP1, 23 CEP2, 45 CEP3, 23 CEP4, 30 noCEP at T1. At T2, six patients reached CEP1, 37 CEP2, 38 CEP3, 35 CEP4, 12 noCEP. For noCEP, the number of sites with PPD > 5 mm increased significantly, and PTL was higher compared to CEP1, CEP2 and CEP3 (p < 0.001).

Conclusions

While achieving CEP1 is possible through comprehensive APT, treating a chronic disease often leads to less ideal CEP2/CEP3. Achieving CEP1, CEP2 or CEP3 after APT made no observable difference regarding PTL.

Trial Registration: The study is registered with the United States National Library of Medicine in the clinical trials database (URL: https://clinicaltrials.gov; NCT03048045)

目的采用4个临床终点（cep）比较主动牙周治疗（APT/T1）和支持牙周治疗（SPC/T2）后（120±12个月）的牙周稳定性。方法1：穿刺袋深度(PPD)≤4mm，无≥4mm处有穿刺出血（BOP），总BOP <；10%;CEP2：无PPD >；4 mm，防喷器或PPD≥6 mm；CEP3:≤4个位点，PPD≥5mm；CEP4:≤5齿，PPD≥5mm。假设cep是相互排斥的，比较患者和牙齿相关参数（如牙周牙齿脱落：PTL）。利用受者工作特征分析预测PTL作为CEP的截止值进行评估。结果128例患者(年龄65.5±10.5岁；III期83人，IV期45人；B级47人，C级81人)，达到CEP1者7人，达到CEP2者23人，达到CEP3者45人，达到CEP4者23人，达到noCEP者30人。T2时，6例达到CEP1, 37例达到CEP2, 38例达到CEP3, 35例达到CEP4, 12例达到noCEP。对于noCEP，具有PPD >；5 mm显著增高，PTL较CEP1、CEP2和CEP3增高(p <；0.001)。结论通过综合APT可以实现CEP1，但慢性疾病的治疗往往导致CEP2/CEP3不理想。APT后达到CEP1、CEP2或CEP3对PTL没有明显差异。试验注册：本研究已在美国国家医学图书馆临床试验数据库注册(URL: https://clinicaltrials.gov；NCT03048045)

{"title":"Clinical Treatment Endpoints After Active Periodontal Treatment and 10 Years of Supportive Periodontal Care: A Retrospective Cohort Study","authors":"Mario Schröder, Max Buchinger, Iulia Dahmer, Peter Eickholz, Hari Petsos","doi":"10.1111/jcpe.14179","DOIUrl":"10.1111/jcpe.14179","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Comparing periodontal stability following active periodontal treatment (APT/T1) and 120 ± 12 months of supportive periodontal care (SPC/T2) using four clinical endpoints (CEPs).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>CEP1: pocket probing depths (PPD) ≤ 4 mm, no sites with ≥ 4 mm with bleeding on probing (BOP), and total BOP < 10%; CEP2: no PPD > 4 mm with BOP or PPD ≥ 6 mm; CEP3: ≤ 4 sites with PPD ≥ 5 mm; CEP4: ≤ 5 teeth with PPD ≥ 5 mm. Assuming CEPs are mutually exclusive, patient- and tooth-related parameters (e.g., periodontal tooth loss: PTL) were compared. Using receiver operating characteristic analysis for the prediction of PTL as a cutoff for CEP was assessed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>From 128 patients (age 65.5 ± 10.5 years; 83 stage III, 45 stage IV; 47 grade B, 81 grade C), 7 achieved CEP1, 23 CEP2, 45 CEP3, 23 CEP4, 30 noCEP at T1. At T2, six patients reached CEP1, 37 CEP2, 38 CEP3, 35 CEP4, 12 noCEP. For noCEP, the number of sites with PPD > 5 mm increased significantly, and PTL was higher compared to CEP1, CEP2 and CEP3 (<i>p</i> < 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>While achieving CEP1 is possible through comprehensive APT, treating a chronic disease often leads to less ideal CEP2/CEP3. Achieving CEP1, CEP2 or CEP3 after APT made no observable difference regarding PTL.</p>\u0000 \u0000 <p>\u0000 <b>Trial Registration:</b> The study is registered with the United States National Library of Medicine in the clinical trials database (URL: https://clinicaltrials.gov; NCT03048045)</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"52 10","pages":"1386-1397"},"PeriodicalIF":6.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.14179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endpoints of Periodontal Therapy in Elderly Patients With Stage III/IV Periodontitis and Their Oral Health–Related Quality of Life Following 10 Years of Supportive Periodontal Therapy 老年III/IV期牙周炎患者的牙周治疗终点及10年牙周支持性治疗后口腔健康相关生活质量

IF 6.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2025-07-06 DOI: 10.1111/jcpe.14198

Caspar Victor Bumm, Sophie Gaenesch, Florian Nagler, Iris Frasheri, Falk Schwendicke, Vinay Pitchika, Christina Ern, Richard Heym, Charlotte Wetzel, Matthias Folwaczny, Nils Werner

Aim

To investigate clinical endpoints of periodontal therapy after steps 1 and 2 of therapy and their association with oral health–related quality of life (OHRQoL) following long-term supportive periodontal therapy (SPT).

Materials and Methods

Forty-seven patients receiving SPT for 126 ± 30 months were included. Clinical endpoints of therapy, as proposed by the EFP (PPD ≤ 3 mm, ≤ 5 mm without bleeding on probing), and a treat-to-target endpoint (T2T; ≤ 4 sites with PPD of ≥ 5 mm) were determined following steps 1 and 2 of therapy (T1) and were associated with patients' OHRQoL using the Oral Health Impact Profile (OHIP)-14 as well as tooth loss (TL) and self-reported tooth migration 126 ± 30 months after step 2 (T2).

Results

One patient achieved the EFP endpoint and 16 achieved T2T, and 30 patients failed to achieve any endpoint at T1. OHRQoL at T2 did not differ significantly between patients achieving or not achieving the endpoints (p = 0.485). Self-reported tooth migration during the examination period was significantly associated with poorer OHRQoL (p = 0.009).

Conclusions

OHRQoL has become a major subject of periodontal research. Within the limitations of this study, achieving clinical endpoints does not seem to affect patients' OHRQoL following long-term SPT. Patients reporting on tooth migration, however, showed significantly reduced OHRQoL. Besides clinical endpoints, functional and aesthetic complaints of periodontally compromised patients should be considered when evaluating the success of therapy.

目的探讨牙周治疗第1步和第2步治疗后的临床终点及其与长期支持牙周治疗（SPT）后口腔健康相关生活质量（OHRQoL）的关系。材料和方法纳入47例接受SPT治疗126±30个月的患者。治疗的临床终点，如EFP提出的（PPD≤3mm,≤5mm，穿刺时不出血）和治疗至目标终点(T2T；在治疗第1步和第2步（T1）后确定PPD≥5 mm的≤4个位点，并通过口腔健康影响量表（OHIP）‐14以及第2步（T2）后126±30个月的牙齿脱落（TL）和自我报告的牙齿迁移与患者的OHRQoL相关。结果1例患者达到EFP终点，16例患者达到T2T， 30例患者在T1时未达到任何终点。达到或未达到终点的患者在T2时的OHRQoL无显著差异（p = 0.485）。检查期间自我报告的牙齿移位与较差的OHRQoL显著相关（p = 0.009）。结论hrqol已成为牙周病研究的重要课题。在本研究的局限性内，达到临床终点似乎并不影响长期SPT后患者的OHRQoL。然而，报告牙齿移位的患者显示OHRQoL显着降低。除了临床终点外，在评估治疗成功时还应考虑牙周受损患者的功能和美观问题。

{"title":"Endpoints of Periodontal Therapy in Elderly Patients With Stage III/IV Periodontitis and Their Oral Health–Related Quality of Life Following 10 Years of Supportive Periodontal Therapy","authors":"Caspar Victor Bumm, Sophie Gaenesch, Florian Nagler, Iris Frasheri, Falk Schwendicke, Vinay Pitchika, Christina Ern, Richard Heym, Charlotte Wetzel, Matthias Folwaczny, Nils Werner","doi":"10.1111/jcpe.14198","DOIUrl":"10.1111/jcpe.14198","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To investigate clinical endpoints of periodontal therapy after steps 1 and 2 of therapy and their association with oral health–related quality of life (OHRQoL) following long-term supportive periodontal therapy (SPT).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Forty-seven patients receiving SPT for 126 ± 30 months were included. Clinical endpoints of therapy, as proposed by the EFP (PPD ≤ 3 mm, ≤ 5 mm without bleeding on probing), and a treat-to-target endpoint (T2T; ≤ 4 sites with PPD of ≥ 5 mm) were determined following steps 1 and 2 of therapy (T1) and were associated with patients' OHRQoL using the Oral Health Impact Profile (OHIP)-14 as well as tooth loss (TL) and self-reported tooth migration 126 ± 30 months after step 2 (T2).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>One patient achieved the EFP endpoint and 16 achieved T2T, and 30 patients failed to achieve any endpoint at T1. OHRQoL at T2 did not differ significantly between patients achieving or not achieving the endpoints (<i>p</i> = 0.485). Self-reported tooth migration during the examination period was significantly associated with poorer OHRQoL (<i>p</i> = 0.009).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>OHRQoL has become a major subject of periodontal research. Within the limitations of this study, achieving clinical endpoints does not seem to affect patients' OHRQoL following long-term SPT. Patients reporting on tooth migration, however, showed significantly reduced OHRQoL. Besides clinical endpoints, functional and aesthetic complaints of periodontally compromised patients should be considered when evaluating the success of therapy.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"52 10","pages":"1398-1409"},"PeriodicalIF":6.8,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.14198","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144568418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

首页上一页

类型

全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

数据库

全部 ACS Publications Elsevier ieeexplore Springer The Royal Society of Chemistry Wiley

期刊

Journal of Clinical Periodontology

全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.

﹀

微信

客服QQ

扫码关注我们

反馈

Book学术文献互助群
群号：604180095

文献互助智能选刊最新文献互助须知联系我们：info@booksci.cn

Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。