Effective prevention of periodontitis requires addressing multiple modifiable health behaviours (MHBs). This study aimed to examine how MHBs interact and to identify the key behaviours that could serve as targets for intervention.
Materials and Methods
We analysed data from a large, population-based survey using questionnaires conducted in China during 2021–2023. Logistic regression was used to examine associations between self-reported periodontitis and multiple MHBs. Network analysis was applied to assess interrelationships among MHBs and identify central MHBs.
Results
Among 85,483 participants, 12.8% reported periodontitis. Ten MHBs were associated with self-reported periodontitis. Ever smoking, ever drinking, unhealthy bedtime, frequent consumption of sugar-sweetened snacks and beverages, insufficient vegetable or fruit intake and toothbrushing less than twice daily were linked to higher prevalence, while insufficient physical activity, no daily flossing and absence of annual dental checkups were linked to lower prevalence. Network analysis identified ever smoking, ever drinking, toothbrushing frequency and dental checkups as the most central behaviours, with variations observed across different subgroups.
Conclusion
This study identified key MHBs and revealed differences in central behaviours across subgroups. Ever smoking, ever drinking, toothbrushing frequency and annual dental checkups may represent priority targets for tailored prevention strategies. Future research is warranted to inform integrated interventions.
{"title":"Network Analysis of Health Behaviours Associated With Periodontitis in Chinese Adults","authors":"Ruiying Fu, Yuting Xie, Huang Huang, Peiyuan Sun, Xiuqi Ma, Zhuolun Hu, Yingtai Chen, Xiaofeng Bi, Qiong Chen, Shaokai Zhang, Yin Liu, Ranran Qie, Jiankun Wei, Miaochang Chen, Jieying Zhong, Zhi Qi, Fan Yao, Lijuan Gao, Huanling Yu, Fen Liu, Yao Zhao, Baozhong Chen, Xiaoli Wei, Shasha Qin, Yuhui Du, Guoyu Zhou, Fangfang Yu, Yue Ba, Tingting Shang, Yaqun Zhang, Shan Zheng, Dongmei Xie, Xiaolan Chen, Xiaoling Liu, Cairong Zhu, Weiwei Wu, Yongliang Feng, Ying Wang, Qi Yan, Mengyao Wu, Yubing Lin, Kaiyong Zou, Yawei Zhang","doi":"10.1111/jcpe.70075","DOIUrl":"10.1111/jcpe.70075","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>Effective prevention of periodontitis requires addressing multiple modifiable health behaviours (MHBs). This study aimed to examine how MHBs interact and to identify the key behaviours that could serve as targets for intervention.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>We analysed data from a large, population-based survey using questionnaires conducted in China during 2021–2023. Logistic regression was used to examine associations between self-reported periodontitis and multiple MHBs. Network analysis was applied to assess interrelationships among MHBs and identify central MHBs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among 85,483 participants, 12.8% reported periodontitis. Ten MHBs were associated with self-reported periodontitis. Ever smoking, ever drinking, unhealthy bedtime, frequent consumption of sugar-sweetened snacks and beverages, insufficient vegetable or fruit intake and toothbrushing less than twice daily were linked to higher prevalence, while insufficient physical activity, no daily flossing and absence of annual dental checkups were linked to lower prevalence. Network analysis identified ever smoking, ever drinking, toothbrushing frequency and dental checkups as the most central behaviours, with variations observed across different subgroups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study identified key MHBs and revealed differences in central behaviours across subgroups. Ever smoking, ever drinking, toothbrushing frequency and annual dental checkups may represent priority targets for tailored prevention strategies. Future research is warranted to inform integrated interventions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 3","pages":"455-465"},"PeriodicalIF":6.8,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145730643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hamoun Sabri, Parham Hazrati, Lorenzo Tavelli, Carlos Garaicoa-Pazmino, Javier Calatrava, Hom-Lay Wang, Shayan Barootchi
<div> <section> <h3> Objective</h3> <p>To investigate the associations between peri-implant phenotype characteristics and long-term outcomes of soft tissue–level implants.</p> </section> <section> <h3> Methods</h3> <p>Twenty-five tissue-level implants from a previous controlled clinical trial were evaluated at 1- and 5-year follow-ups after crown delivery. Data included ultrasonographic scans (mucosal thickness and supracrestal tissue height), standardised 2D radiographs, cone beam computed tomography, clinical outcomes (mucosal recession, probing pocket depth, bleeding on probing), patient-reported outcomes and peri-implant health status. Standard logistic and linear regression models were used to analyse associations of implant- and patient-related factors with outcomes, including peri-implant disease status, mucosal recession and marginal bone level (MBL) changes.</p> </section> <section> <h3> Results</h3> <p>Five-year implant survival was 100%, with peri-implant mucositis diagnosed in 36% of the implants. Mean MBL changes from the first to the fifth year was 0.29 ± 0.29 mm. Baseline (pre-surgical) buccal soft-tissue thickness < 1.5 mm (OR: 5.20, <i>p</i> = 0.007) and increased plaque scores (OR: 30.11, <i>p</i> < 0.001) were associated with peri-implant mucositis, whereas buccal bone thickness ≥ 1.5 mm (OR: 0.48, <i>p</i> = 0.039), mucosal thickness around implant (OR: 0.20, <i>p</i> = 0.001) and supracrestal tissue height (OR: 0.50, <i>p</i> = 0.002) emerged as protective factors. Mucosal recession was significantly associated with baseline buccal soft-tissue thickness < 1.5 mm (estimate = 0.27, <i>p</i> = 0.03) and keratinised mucosa width of < 2 mm (estimate = 0.39, <i>p</i> = 0.021). Clinical thresholds associated with long-term peri-implant health were defined as follows: supracrestal tissue height ≥ 2.8 mm, restorative emergence angle ≤ 35.5°, mucosal thickness 1.8 mm and buccal bone thickness 1.5 mm. Patients reported a high overall satisfaction (visual analogue scale: 88.2%). Colour Doppler ultrasonography showed a strong correlation between tissue perfusion and peri-implant disease (<i>r</i> = 0.93, <i>p</i> < 0.001).</p> </section> <section> <h3> Conclusions</h3> <p>Within the limitations of this study, tissue-level implants showed excellent survival rates and patient satisfaction at 5 years. Several peri-implant parameters—such as supracrestal tissue height ≥ 2.8 mm, restorative emergence angle ≤ 35.5° and buccal bone thickness ≥ 1.5 mm—were associated with favourable outcomes. These exploratory thresholds may be consi
目的探讨软组织水平种植体的种植体周围表型特征与长期预后之间的关系。方法在冠植入后1年和5年的随访中，对来自先前对照临床试验的25个组织级种植体进行评估。数据包括超声扫描（粘膜厚度和阴茎上组织高度）、标准化二维x线片、锥束计算机断层扫描、临床结果（粘膜消退、探查袋深度、探查时出血）、患者报告的结果和种植体周围的健康状况。采用标准logistic和线性回归模型分析种植体和患者相关因素与结果的相关性，包括种植体周围疾病状态、粘膜消退和边缘骨水平（MBL）变化。结果种植体5年生存率为100%，36%的种植体被诊断为种植体周围粘膜炎。第一至第五年MBL平均变化为0.29±0.29 mm。基线（术前）颊软组织厚度1.5 mm （OR: 5.20, p = 0.007）和增加的菌斑评分（OR: 30.11, p = 0.001）与种植体周围粘膜炎相关，而颊骨厚度≥1.5 mm （OR: 0.48, p = 0.039）、种植体周围粘膜厚度（OR: 0.20, p = 0.001）和切上组织高度（OR: 0.50, p = 0.002）成为保护因素。粘膜退缩与基线颊软组织厚度1.5 mm（估计= 0.27,p = 0.03）和角化粘膜宽度2 mm（估计= 0.39,p = 0.021）显著相关。与种植体周围长期健康相关的临床阈值定义如下：切上组织高度≥2.8 mm，修复体出牙角≤35.5°，粘膜厚度1.8 mm，颊骨厚度1.5 mm。患者报告了较高的总体满意度（视觉模拟量表：88.2%）。彩色多普勒超声显示组织灌注与种植体周围疾病有很强的相关性（r = 0.93, p < 0.001）。结论：在本研究的局限性内，组织级植入物在5年内具有良好的生存率和患者满意度。几个种植体周围参数，如切上组织高度≥2.8 mm，修复体涌现角≤35.5°和颊骨厚度≥1.5 mm，与良好的结果相关。这些探索性阈值可能被认为是假设生成，可以帮助指导未来的研究和临床监测，尽管在更大的队列中验证是必不可少的。试验注册：www.clinicaltrials.gov/study/NCT02925078
{"title":"Impact of Peri-Implant Phenotype on Implant Therapy Outcomes: A 5-Year Cohort Analysis on Soft Tissue—Level Implants","authors":"Hamoun Sabri, Parham Hazrati, Lorenzo Tavelli, Carlos Garaicoa-Pazmino, Javier Calatrava, Hom-Lay Wang, Shayan Barootchi","doi":"10.1111/jcpe.70062","DOIUrl":"10.1111/jcpe.70062","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To investigate the associations between peri-implant phenotype characteristics and long-term outcomes of soft tissue–level implants.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Twenty-five tissue-level implants from a previous controlled clinical trial were evaluated at 1- and 5-year follow-ups after crown delivery. Data included ultrasonographic scans (mucosal thickness and supracrestal tissue height), standardised 2D radiographs, cone beam computed tomography, clinical outcomes (mucosal recession, probing pocket depth, bleeding on probing), patient-reported outcomes and peri-implant health status. Standard logistic and linear regression models were used to analyse associations of implant- and patient-related factors with outcomes, including peri-implant disease status, mucosal recession and marginal bone level (MBL) changes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Five-year implant survival was 100%, with peri-implant mucositis diagnosed in 36% of the implants. Mean MBL changes from the first to the fifth year was 0.29 ± 0.29 mm. Baseline (pre-surgical) buccal soft-tissue thickness < 1.5 mm (OR: 5.20, <i>p</i> = 0.007) and increased plaque scores (OR: 30.11, <i>p</i> < 0.001) were associated with peri-implant mucositis, whereas buccal bone thickness ≥ 1.5 mm (OR: 0.48, <i>p</i> = 0.039), mucosal thickness around implant (OR: 0.20, <i>p</i> = 0.001) and supracrestal tissue height (OR: 0.50, <i>p</i> = 0.002) emerged as protective factors. Mucosal recession was significantly associated with baseline buccal soft-tissue thickness < 1.5 mm (estimate = 0.27, <i>p</i> = 0.03) and keratinised mucosa width of < 2 mm (estimate = 0.39, <i>p</i> = 0.021). Clinical thresholds associated with long-term peri-implant health were defined as follows: supracrestal tissue height ≥ 2.8 mm, restorative emergence angle ≤ 35.5°, mucosal thickness 1.8 mm and buccal bone thickness 1.5 mm. Patients reported a high overall satisfaction (visual analogue scale: 88.2%). Colour Doppler ultrasonography showed a strong correlation between tissue perfusion and peri-implant disease (<i>r</i> = 0.93, <i>p</i> < 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Within the limitations of this study, tissue-level implants showed excellent survival rates and patient satisfaction at 5 years. Several peri-implant parameters—such as supracrestal tissue height ≥ 2.8 mm, restorative emergence angle ≤ 35.5° and buccal bone thickness ≥ 1.5 mm—were associated with favourable outcomes. These exploratory thresholds may be consi","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 3","pages":"394-406"},"PeriodicalIF":6.8,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145730641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Denis Bourgeois, Aida Kanoute, Daouda Faye, Marta Mazur, Hervé Perrier, Roman Ardan, Céline Clément, Lucio Souza Gonçalves, Romain Lan, Florence Carrouel
Aim
Pregnancy gingivitis is potentially modifiable, making it a promising target for preventive strategies to reduce adverse obstetrical outcomes. The primary objective of this study was to evaluate whether an interdental hygiene intervention in early pregnancy could reduce obstetrical complications. Exploratory analyses focused on potential biological pathways linking oral inflammation and obstetrical outcomes.
Materials and Methods
A two-arm randomised controlled trial (RCT) with cluster allocation (1:1) was conducted in six antenatal clinics in Senegal among nulliparous women at 12 weeks. Participants received either a daily calibrated interdental brushing intervention (Intervention) or usual oral hygiene (Control). Follow-up visits were scheduled at 5 and 8 months. The primary endpoint was a composite of preterm birth, low birth weight, small for gestational age or pre-eclampsia, analysed exploratorily under the intention-to-treat principle.
Results
A total of 323 women were randomised (162 intervention, 161 control). The composite outcome occurred in 28.4% of the intervention group and 24.2% of the control group (p = 0.449). After adjustment, the intervention was not significantly associated (aOR = 0.65; 95% CI: 0.39–1.10; p = 0.107). Exploratory analyses suggested that interdental bleeding and C-reactive protein (CRP) could act as mediators, accounting for ~60% of the effect, with the remaining ~40% representing a direct effect. No intervention-related adverse events were observed.
Conclusion
This RCT did not demonstrate a significant reduction in obstetrical complications with early interdental prophylaxis. However, the intervention was safe, acceptable and feasible within antenatal care, highlighting the need for adequately powered trials to clarify its potential clinical impact.
{"title":"Interdental Brushing and Obstetrical Outcomes in Nulliparous Pregnant Women: Insights From a Cluster Randomised Controlled Trial","authors":"Denis Bourgeois, Aida Kanoute, Daouda Faye, Marta Mazur, Hervé Perrier, Roman Ardan, Céline Clément, Lucio Souza Gonçalves, Romain Lan, Florence Carrouel","doi":"10.1111/jcpe.70070","DOIUrl":"10.1111/jcpe.70070","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>Pregnancy gingivitis is potentially modifiable, making it a promising target for preventive strategies to reduce adverse obstetrical outcomes. The primary objective of this study was to evaluate whether an interdental hygiene intervention in early pregnancy could reduce obstetrical complications. Exploratory analyses focused on potential biological pathways linking oral inflammation and obstetrical outcomes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A two-arm randomised controlled trial (RCT) with cluster allocation (1:1) was conducted in six antenatal clinics in Senegal among nulliparous women at 12 weeks. Participants received either a daily calibrated interdental brushing intervention (Intervention) or usual oral hygiene (Control). Follow-up visits were scheduled at 5 and 8 months. The primary endpoint was a composite of preterm birth, low birth weight, small for gestational age or pre-eclampsia, analysed exploratorily under the intention-to-treat principle.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 323 women were randomised (162 intervention, 161 control). The composite outcome occurred in 28.4% of the intervention group and 24.2% of the control group (<i>p</i> = 0.449). After adjustment, the intervention was not significantly associated (aOR = 0.65; 95% CI: 0.39–1.10; <i>p</i> = 0.107). Exploratory analyses suggested that interdental bleeding and C-reactive protein (CRP) could act as mediators, accounting for ~60% of the effect, with the remaining ~40% representing a direct effect. No intervention-related adverse events were observed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This RCT did not demonstrate a significant reduction in obstetrical complications with early interdental prophylaxis. However, the intervention was safe, acceptable and feasible within antenatal care, highlighting the need for adequately powered trials to clarify its potential clinical impact.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 3","pages":"436-444"},"PeriodicalIF":6.8,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145710895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Li Niu, Xiaojing Ke, Wei Zhou, Baochun Tan, Junjie Zhao, Bin Chen, Yanfen Li, Peng Zhang, Fuhua Yan, Houxuan Li
Aim
To assess a new material in comparison with free gingival graft (FGG) for increasing the width of keratinised tissue (KT).
Material and Methods
Forty-six participants were randomly allocated to the absorbable gradient membrane (AGM) or the FGG group. This trial used inter-patient comparison to establish the non-inferiority of AGM compared to FGG. The primary outcome (KT) was examined from baseline to 3 years after surgery. Secondary outcomes included the plaque index (PI), bleeding index (BI), gingival recession (GR), probing depth (PD), immunoglobulin E (IgE) level, postoperative pain, aesthetics and patient satisfaction.
Results
The width of KT in the AGM group was non-inferior to that of the FGG group at all short-term follow-ups (1, 3 and 6 months), with a pre-defined margin of 1 mm. However, this non-inferiority disappeared by 3 years after operation. From 6 months to 3 years, the GR associated with FGG significantly decreased, while that associated with AGM showed no significant change between adjacent time points. No significant differences were observed in PI, BI, PD or IgE levels between the groups. AGM required shorter surgery times and secured better aesthetic outcomes than FGG.
Conclusion
AGM showed short-term non-inferiority to FGG for KT augmentation. However, the non-inferiority was not sustained at 3 years.
Trial Registration
The study was registered with the China Clinical Trial Center under ChiCTR2000034683. Informed consent was obtained from all participants
{"title":"Clinical Evaluation of Absorbable Gradient Membrane vs. Free Gingival Grafts for Periodontal Soft-Tissue Augmentation: A Randomised Controlled Clinical Trial","authors":"Li Niu, Xiaojing Ke, Wei Zhou, Baochun Tan, Junjie Zhao, Bin Chen, Yanfen Li, Peng Zhang, Fuhua Yan, Houxuan Li","doi":"10.1111/jcpe.70073","DOIUrl":"10.1111/jcpe.70073","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To assess a new material in comparison with free gingival graft (FGG) for increasing the width of keratinised tissue (KT).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Forty-six participants were randomly allocated to the absorbable gradient membrane (AGM) or the FGG group. This trial used inter-patient comparison to establish the non-inferiority of AGM compared to FGG. The primary outcome (KT) was examined from baseline to 3 years after surgery. Secondary outcomes included the plaque index (PI), bleeding index (BI), gingival recession (GR), probing depth (PD), immunoglobulin E (IgE) level, postoperative pain, aesthetics and patient satisfaction.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The width of KT in the AGM group was non-inferior to that of the FGG group at all short-term follow-ups (1, 3 and 6 months), with a pre-defined margin of 1 mm. However, this non-inferiority disappeared by 3 years after operation. From 6 months to 3 years, the GR associated with FGG significantly decreased, while that associated with AGM showed no significant change between adjacent time points. No significant differences were observed in PI, BI, PD or IgE levels between the groups. AGM required shorter surgery times and secured better aesthetic outcomes than FGG.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>AGM showed short-term non-inferiority to FGG for KT augmentation. However, the non-inferiority was not sustained at 3 years.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Trial Registration</h3>\u0000 \u0000 <p>The study was registered with the China Clinical Trial Center under ChiCTR2000034683. Informed consent was obtained from all participants</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 3","pages":"373-383"},"PeriodicalIF":6.8,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145674128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To identify shared genetic architectures and molecular pathways underlying the frequent co-occurrence of stage III/IV grade C periodontitis under 35 years of age (PIII/IV-C< 35y) and abnormal root morphology (ARM), using a family-based SNP set and transcriptomic approach.
Materials and Methods
We conducted a family-based study using whole-genome genotyping of 148 Han Chinese individuals from 52 families, including 52 probands, 35 mothers, 32 fathers and 29 siblings. The participants included 65 comorbidity cases, 29 PIII/IV-C< 35y-only cases, 27 ARM-only cases and 27 controls. Whole-genome genotyping and bi-clustering were used to identify SNP sets associated with each phenotype, and transcriptomic profiling was performed to validate gene expression changes.
Results
Among 109 SNP sets, 91 (83.5%) showed ≥ 40% comorbidity risk. Fifty-four and 27 SNP sets were significantly associated with PIII/IV-C< 35y and ARM, respectively. Six genotype subnetworks were enriched for neural development and inflammation pathways, particularly IL-17 signalling. Transcriptome data confirmed down-regulation of KLHL29, HSF2 and COL13A1 in PIII/IV-C< 35y, and a reduction in GJA1 expression in comorbidity cases.
Conclusions
This study reveals a shared genetic architecture between PIII/IV-C< 35y and ARM, implicating neural development and inflammatory pathways in their co-occurrence. Key genes such as GJA1 may advance mechanistic understanding and support early identification of high-risk individuals.
{"title":"Shared Genotypic Architectures of Stage III/IV Grade C Periodontitis Under 35 Years of Age (Former Aggressive Periodontitis) and Abnormal Root Morphology: A Family-Based Study","authors":"Xiaoyuan Guan, Xiaoyi Li, Dafang Chen, Li Xu, Wenjing Li, Ruifang Lu, Xian'e Wang, Huanxin Meng","doi":"10.1111/jcpe.70071","DOIUrl":"10.1111/jcpe.70071","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To identify shared genetic architectures and molecular pathways underlying the frequent co-occurrence of stage III/IV grade C periodontitis under 35 years of age (PIII/IV-C< 35y) and abnormal root morphology (ARM), using a family-based SNP set and transcriptomic approach.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>We conducted a family-based study using whole-genome genotyping of 148 Han Chinese individuals from 52 families, including 52 probands, 35 mothers, 32 fathers and 29 siblings. The participants included 65 comorbidity cases, 29 PIII/IV-C< 35y-only cases, 27 ARM-only cases and 27 controls. Whole-genome genotyping and bi-clustering were used to identify SNP sets associated with each phenotype, and transcriptomic profiling was performed to validate gene expression changes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among 109 SNP sets, 91 (83.5%) showed ≥ 40% comorbidity risk. Fifty-four and 27 SNP sets were significantly associated with PIII/IV-C< 35y and ARM, respectively. Six genotype subnetworks were enriched for neural development and inflammation pathways, particularly IL-17 signalling. Transcriptome data confirmed down-regulation of <i>KLHL29</i>, <i>HSF2</i> and <i>COL13A1</i> in PIII/IV-C< 35y, and a reduction in <i>GJA1</i> expression in comorbidity cases.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This study reveals a shared genetic architecture between PIII/IV-C< 35y and ARM, implicating neural development and inflammatory pathways in their co-occurrence. Key genes such as <i>GJA1</i> may advance mechanistic understanding and support early identification of high-risk individuals.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 3","pages":"478-491"},"PeriodicalIF":6.8,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145680105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To compare acute-phase response and blood pressure levels following full-mouth or quadrant non-surgical periodontal treatment (FM-SRP or Q-SRP) in patients affected by hypertension and periodontitis.
Patients and Methods
Forty patients affected by hypertension and periodontitis were enrolled and randomly allocated to either the FM-SRP or Q-SRP group. Periodontal parameters were registered at baseline and 3 months after treatment. Blood samples and vital signs were collected at baseline, 24 h and 3 months after treatment. Primary outcomes were high-sensitivity C-reactive protein (hs-CRP) concentration 24 h post operation and the variation in levels of blood pressure (BP).
Results
Significant increase in hs-CRP levels was observed at 24 h, with FM-SRP presenting higher values than Q-SRP (p < 0.05). In terms of periodontal outcomes, both treatment regimens proved equally effective. Systolic BP was significantly reduced at days 1 and 90 in both groups (p < 0.01), while a 90-day significant decrease in diastolic BP was detected only in the Q-SRP group (p < 0.01). Multiple regression analysis suggests that the peak of hs-CRP at 24 h may influence BP reduction at Day 90.
Conclusions
Q-SRP may be preferred in patients affected by hypertension and periodontitis, as a higher post-operative inflammatory response was associated with lower improvements in BP control in the medium term.
{"title":"Acute-Phase Response Following Different Modalities of Non-Surgical Periodontal Treatment in Subjects Affected by Periodontitis and Co-Morbid Hypertension: A Randomised Clinical Trial","authors":"Morena Petrini, Stefano Gennai, Crystal Marruganti, Urska Marhl, Rossana Izzetti, Marco Nisi, Filippo Graziani","doi":"10.1111/jcpe.70068","DOIUrl":"10.1111/jcpe.70068","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To compare acute-phase response and blood pressure levels following full-mouth or quadrant non-surgical periodontal treatment (FM-SRP or Q-SRP) in patients affected by hypertension and periodontitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Patients and Methods</h3>\u0000 \u0000 <p>Forty patients affected by hypertension and periodontitis were enrolled and randomly allocated to either the FM-SRP or Q-SRP group. Periodontal parameters were registered at baseline and 3 months after treatment. Blood samples and vital signs were collected at baseline, 24 h and 3 months after treatment. Primary outcomes were high-sensitivity C-reactive protein (hs-CRP) concentration 24 h post operation and the variation in levels of blood pressure (BP).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant increase in hs-CRP levels was observed at 24 h, with FM-SRP presenting higher values than Q-SRP (<i>p</i> < 0.05). In terms of periodontal outcomes, both treatment regimens proved equally effective. Systolic BP was significantly reduced at days 1 and 90 in both groups (<i>p</i> < 0.01), while a 90-day significant decrease in diastolic BP was detected only in the Q-SRP group (<i>p</i> < 0.01). Multiple regression analysis suggests that the peak of hs-CRP at 24 h may influence BP reduction at Day 90.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Q-SRP may be preferred in patients affected by hypertension and periodontitis, as a higher post-operative inflammatory response was associated with lower improvements in BP control in the medium term.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 3","pages":"350-361"},"PeriodicalIF":6.8,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isabel Lopez-Oliva, Iain L. Chapple, Akshay Paropkari, Shweta Saraswat, Praveen Sharma, Stefan Serban, Paola de Pablo, Karim Raza, Andrew Filer, Thomas Dietrich, Melissa Grant, Purnima S. Kumar
Aim
To explore mechanistic links between rheumatoid arthritis (RA) and periodontitis (PD) through the lens of subgingival microbial dysbiosis–mediated inflammation.
Methods
Subgingival plaque from 100 volunteers with RA and PD (RAPD), 22 with RA (RAnoPD), 18 with PD (PDnoRA) and 19 healthy controls (noRAnoPD) was analysed using 16S-amplicon sequencing, semi-quantitative bead-based flow cytometry to measure crevicular fluid cytokines and ELISA to quantify antibodies to oral pathogens and systemic inflammatory markers in serum. The RAPD group had been randomised to receive intensive non-surgical periodontal therapy (PMPR) or oral hygiene alone and reviewed at 3 and 6 months in our previously reported study.
Results
Subgingival microbial dysbiosis, as evidenced by higher species richness, alpha-diversity and higher levels of known and putative periodontal pathobionts, was evident at baseline in RAnoPD, RAPD and PDnoRA. Higher serum antibodies to oral pathogens were recorded in RAPD. PMPR restored host–microbial homeostasis in RAPD within 3 months. Significant decreases in serum antibodies to microbial antigens and clinical measures of RA activity were seen after 3 and 6 months in the PMPR group but not controls.
Conclusions
We demonstrate a mutualistic influence of RA and PD, beginning with RA-induced dysbiosis of the periodontal microbiome, progressing to periodontal inflammation and culminating in PD-driven exacerbation of systemic inflammation.
{"title":"Dysbiosis-Mediated Inflammation: A Pathophysiological Link Between Rheumatoid Arthritis and Periodontitis","authors":"Isabel Lopez-Oliva, Iain L. Chapple, Akshay Paropkari, Shweta Saraswat, Praveen Sharma, Stefan Serban, Paola de Pablo, Karim Raza, Andrew Filer, Thomas Dietrich, Melissa Grant, Purnima S. Kumar","doi":"10.1111/jcpe.70063","DOIUrl":"10.1111/jcpe.70063","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To explore mechanistic links between rheumatoid arthritis (RA) and periodontitis (PD) through the lens of subgingival microbial dysbiosis–mediated inflammation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Subgingival plaque from 100 volunteers with RA and PD (RAPD), 22 with RA (RAnoPD), 18 with PD (PDnoRA) and 19 healthy controls (noRAnoPD) was analysed using 16S-amplicon sequencing, semi-quantitative bead-based flow cytometry to measure crevicular fluid cytokines and ELISA to quantify antibodies to oral pathogens and systemic inflammatory markers in serum. The RAPD group had been randomised to receive intensive non-surgical periodontal therapy (PMPR) or oral hygiene alone and reviewed at 3 and 6 months in our previously reported study.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Subgingival microbial dysbiosis, as evidenced by higher species richness, alpha-diversity and higher levels of known and putative periodontal pathobionts, was evident at baseline in RAnoPD, RAPD and PDnoRA. Higher serum antibodies to oral pathogens were recorded in RAPD. PMPR restored host–microbial homeostasis in RAPD within 3 months. Significant decreases in serum antibodies to microbial antigens and clinical measures of RA activity were seen after 3 and 6 months in the PMPR group but not controls.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We demonstrate a mutualistic influence of RA and PD, beginning with RA-induced dysbiosis of the periodontal microbiome, progressing to periodontal inflammation and culminating in PD-driven exacerbation of systemic inflammation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 3","pages":"466-477"},"PeriodicalIF":6.8,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145656973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Emilio Couso-Queiruga, Miguel Padial-Molina, Pablo Galindo-Moreno, Carlos Garaicoa-Pazmino, Nicolly Oliveira-Santos, Giuseppe Troiano, Vivianne Chappuis, Gustavo Avila-Ortiz
Aim
To evaluate the effect of specific phenotypical and anatomical features of the alveolar process and basal bone on post-extraction dimensional changes.
Materials and Methods
Patients requiring the extraction of a single maxillary non-molar tooth were enrolled. A comprehensive panel of baseline anatomical characteristics, including sagittal root position (SRP) class, was recorded. Linear and volumetric bone changes and the need for ancillary bone augmentation (NBA) were assessed after a 14-week healing period.
Results
Records from 65 patients were included. Facial bone thickness (FBT) ≤ 1 mm was associated with significantly greater dimensional changes and greater NBA compared to sites presenting FBT > 1 mm (88.46% vs. 35.13%; p < 0.002). Bone remodelling and NBA were highest in SRP Class IV (100%), with lower frequencies in Class I (60%) and Class II (43.5%). Sites with roots outside the bony housing showed significantly more remodelling and NBA (89.47% vs. 43.18%; p < 0.001). Sites with a basal bone width narrower than the alveolar processes exhibited significantly greater bone remodelling and a higher prevalence of NBA compared to sites where the basal bone was wider (88.89% vs. 50%; p = 0.09). Socket entrance dimensions, distance from the coronal aspect of the facial bone to adjacent boundaries and root volume showed a comparable trend towards increased dimensional changes and grafting.
Conclusions
Certain site-specific features appear to significantly affect post-extraction alveolar ridge remodelling and the subsequent NBA to facilitate implant therapy.
目的探讨牙槽突和基底骨的特定表型和解剖特征对拔牙后尺寸变化的影响。材料和方法选择需要拔除单颗上颌非磨牙的患者。全面的基线解剖特征，包括矢状根位置（SRP）分类，被记录。在14周的愈合期后，评估线性和体积骨变化以及辅助骨增强（NBA）的需求。结果纳入65例患者的记录。与面骨厚度≤1 mm的部位相比，面骨厚度≤1 mm的部位有更大的尺寸变化和更大的NBA （88.46% vs. 35.13%; p < 0.002）。骨重塑和NBA在SRP IV类中发生率最高（100%），在I类和II类中发生率较低（60%）（43.5%）。根在骨壳外的部位表现出更多的重塑和NBA （89.47% vs. 43.18%; p < 0.001）。与基底骨较宽的部位相比，基底骨宽度小于牙槽突的部位表现出更大的骨重塑和更高的NBA患病率（88.89%比50%；p = 0.09）。窝口尺寸、从面骨冠状面到相邻边界的距离和根体积都显示出类似的尺寸变化和嫁接增加的趋势。结论：某些特定部位的特征似乎显著影响拔牙后牙槽嵴重塑和随后的NBA，以促进种植治疗。
{"title":"Effect of Alveolar Process and Basal Bone Features on Post-Extraction Dimensional Changes","authors":"Emilio Couso-Queiruga, Miguel Padial-Molina, Pablo Galindo-Moreno, Carlos Garaicoa-Pazmino, Nicolly Oliveira-Santos, Giuseppe Troiano, Vivianne Chappuis, Gustavo Avila-Ortiz","doi":"10.1111/jcpe.70069","DOIUrl":"10.1111/jcpe.70069","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To evaluate the effect of specific phenotypical and anatomical features of the alveolar process and basal bone on post-extraction dimensional changes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Patients requiring the extraction of a single maxillary non-molar tooth were enrolled. A comprehensive panel of baseline anatomical characteristics, including sagittal root position (SRP) class, was recorded. Linear and volumetric bone changes and the need for ancillary bone augmentation (NBA) were assessed after a 14-week healing period.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Records from 65 patients were included. Facial bone thickness (FBT) ≤ 1 mm was associated with significantly greater dimensional changes and greater NBA compared to sites presenting FBT > 1 mm (88.46% vs. 35.13%; <i>p</i> < 0.002). Bone remodelling and NBA were highest in SRP Class IV (100%), with lower frequencies in Class I (60%) and Class II (43.5%). Sites with roots outside the bony housing showed significantly more remodelling and NBA (89.47% vs. 43.18%; <i>p</i> < 0.001). Sites with a basal bone width narrower than the alveolar processes exhibited significantly greater bone remodelling and a higher prevalence of NBA compared to sites where the basal bone was wider (88.89% vs. 50%; <i>p</i> = 0.09). Socket entrance dimensions, distance from the coronal aspect of the facial bone to adjacent boundaries and root volume showed a comparable trend towards increased dimensional changes and grafting.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Certain site-specific features appear to significantly affect post-extraction alveolar ridge remodelling and the subsequent NBA to facilitate implant therapy.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 3","pages":"384-393"},"PeriodicalIF":6.8,"publicationDate":"2025-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shuyu Cheng, Shiyuan Song, Wen Zhang, Dan Qiao, Min Wang, Zhifeng Wang, Jiajie Pu, Wenrong Yang, Fuhua Yan, Yangheng Zhang
Aim To investigate the association between tryptophan‐derived microbial metabolites and periodontitis, and to assess whether the differential tryptophan metabolite indole‐3‐acetic acid (IAA) could ameliorate periodontitis and elucidate its potential mechanism. Materials and Methods The association between tryptophan‐derived metabolites and periodontitis was evaluated using targeted metabolomics analysis, 16S rRNA gene amplicon sequencing and correlation analysis. The impact of the differential metabolite IAA on periodontitis was assessed with micro‐computed tomography (micro‐CT), histological staining and real‐time quantitative polymerase chain reaction (RT‐qPCR). The effects of IAA on macrophages under an inflammatory microenvironment, along with its potential mechanism, were explored using RT‐qPCR, enzyme‐linked immunosorbent assay (ELISA), immunofluorescence staining and Western blot. Results IAA, a differential tryptophan metabolite, was identified in the saliva of patients with periodontitis and periodontally healthy individuals. Local application of IAA in experimental mice with periodontitis alleviated the immune‐inflammatory response and reduced periodontal tissue destruction. Mechanistically, IAA induced M2 macrophage polarisation by activating the AhR/CYP1A1 axis, thereby contributing to the amelioration of periodontal destruction. Conclusions These findings highlight the potential role of the microbial metabolite IAA in the development and treatment of periodontitis, offering a new perspective for intervention strategies based on microbial metabolites for periodontitis.
{"title":"Tryptophan‐Derived Microbial Metabolite Indole‐3‐Acetic Acid Ameliorates Periodontitis Through AhR / CYP1A1 ‐Mediated Macrophage Polarisation","authors":"Shuyu Cheng, Shiyuan Song, Wen Zhang, Dan Qiao, Min Wang, Zhifeng Wang, Jiajie Pu, Wenrong Yang, Fuhua Yan, Yangheng Zhang","doi":"10.1111/jcpe.70064","DOIUrl":"https://doi.org/10.1111/jcpe.70064","url":null,"abstract":"Aim To investigate the association between tryptophan‐derived microbial metabolites and periodontitis, and to assess whether the differential tryptophan metabolite indole‐3‐acetic acid (IAA) could ameliorate periodontitis and elucidate its potential mechanism. Materials and Methods The association between tryptophan‐derived metabolites and periodontitis was evaluated using targeted metabolomics analysis, 16S rRNA gene amplicon sequencing and correlation analysis. The impact of the differential metabolite IAA on periodontitis was assessed with micro‐computed tomography (micro‐CT), histological staining and real‐time quantitative polymerase chain reaction (RT‐qPCR). The effects of IAA on macrophages under an inflammatory microenvironment, along with its potential mechanism, were explored using RT‐qPCR, enzyme‐linked immunosorbent assay (ELISA), immunofluorescence staining and Western blot. Results IAA, a differential tryptophan metabolite, was identified in the saliva of patients with periodontitis and periodontally healthy individuals. Local application of IAA in experimental mice with periodontitis alleviated the immune‐inflammatory response and reduced periodontal tissue destruction. Mechanistically, IAA induced M2 macrophage polarisation by activating the AhR/CYP1A1 axis, thereby contributing to the amelioration of periodontal destruction. Conclusions These findings highlight the potential role of the microbial metabolite IAA in the development and treatment of periodontitis, offering a new perspective for intervention strategies based on microbial metabolites for periodontitis.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"88 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145592848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiao Wu, Jiawei Lu, Zehui Xiong, Jie Huang, Jinyi Zhang, Lijun Luo
Aim To investigate the impact of neutrophil extracellular traps (NETs) on periodontitis and the regulatory mechanisms by which melatonin alleviates the disease. Materials and Methods The expression of NET markers in periodontitis tissues was detected using multiple immunohistochemical staining. The effect of NETs on periodontitis was explored through in vivo and in vitro experiments. qPCR and transwell migration assay were used to assess neutrophil activation and recruitment. Flow cytometry, micro‐CT and histological staining were employed to evaluate neutrophil recruitment in gingiva and periodontitis progression in a ligature‐induced periodontitis (LIP) mouse model. Melatonin was then administered in vivo and in vitro to evaluate its effects on NET formation, neutrophil activation, neutrophil recruitment and periodontitis progression. Finally, Western blotting and qPCR were employed to elucidate the mechanisms underlying melatonin‐mediated regulation of NET formation. Results NETs were evident in gingival tissues from patients with periodontitis. After inhibiting NET formation, the expression levels of inflammatory cytokines (Il‐1β, Il‐6, Tnf‐α), neutrophil chemokines (Ccl2, Ccl4, Cxcl1) and neutrophil recruitment were found to decrease. Furthermore, melatonin treatment reduced NET formation, decreased neutrophil activation and recruitment and alleviated bone resorption in LIP mice. Mechanistically, melatonin suppresses NET formation through the NF‐κB signalling pathway. Conclusions Melatonin decreases neutrophil recruitment by suppressing NET formation, exerting a protective effect in periodontitis.
{"title":"Melatonin Alleviates Periodontitis by Suppressing Neutrophil Extracellular Traps–Dependent Neutrophil Recruitment","authors":"Xiao Wu, Jiawei Lu, Zehui Xiong, Jie Huang, Jinyi Zhang, Lijun Luo","doi":"10.1111/jcpe.70067","DOIUrl":"https://doi.org/10.1111/jcpe.70067","url":null,"abstract":"Aim To investigate the impact of neutrophil extracellular traps (NETs) on periodontitis and the regulatory mechanisms by which melatonin alleviates the disease. Materials and Methods The expression of NET markers in periodontitis tissues was detected using multiple immunohistochemical staining. The effect of NETs on periodontitis was explored through in vivo and in vitro experiments. qPCR and transwell migration assay were used to assess neutrophil activation and recruitment. Flow cytometry, micro‐CT and histological staining were employed to evaluate neutrophil recruitment in gingiva and periodontitis progression in a ligature‐induced periodontitis (LIP) mouse model. Melatonin was then administered in vivo and in vitro to evaluate its effects on NET formation, neutrophil activation, neutrophil recruitment and periodontitis progression. Finally, Western blotting and qPCR were employed to elucidate the mechanisms underlying melatonin‐mediated regulation of NET formation. Results NETs were evident in gingival tissues from patients with periodontitis. After inhibiting NET formation, the expression levels of inflammatory cytokines (Il‐1β, Il‐6, Tnf‐α), neutrophil chemokines (Ccl2, Ccl4, Cxcl1) and neutrophil recruitment were found to decrease. Furthermore, melatonin treatment reduced NET formation, decreased neutrophil activation and recruitment and alleviated bone resorption in LIP mice. Mechanistically, melatonin suppresses NET formation through the NF‐κB signalling pathway. Conclusions Melatonin decreases neutrophil recruitment by suppressing NET formation, exerting a protective effect in periodontitis.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"167 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145593441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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