To discover new salivary biomarkers to diagnose periodontitis and evaluate the impact of age and smoking on predictive capacity.
Saliva samples were collected from 44 healthy periodontal individuals and 41 with periodontitis. Samples were analysed by sequential window acquisition of all theoretical mass spectra (SWATH-MS), and proteins were identified by employing the UniProt database. The diagnostic capacity of the molecules was determined with generalized additive models. The models obtained were single-protein unadjusted and adjusted for age and smoking status, besides two-protein combinations.
Eight single salivary proteins had a bias-corrected accuracy (bc-ACC) of 78.8%–86.8% (bc-sensitivity/bc-specificity of 62.5%–86.9%/60.9%–98.1%) to diagnose periodontitis. Predictive capacity increased more by adjusting for age (bc-ACC: 94.1%–98.2%; bc-sensitivity/bc-specificity: 90.2%–98.6%/93.6%–97.2%) than smoking (bc-ACC: 83.9%–90.4%; bc-sensitivity/bc-specificity: 73.6%–89.9%/76.2%–96.4%). These proteins were keratin, type II cytoskeletal 1, protein S100-A8, β-2-microglobulin, neutrophil defensin 1, lysozyme C, ubiquitin-60S ribosomal protein L40, isoform 2 of tropomyosin α-3 chain and resistin. Two dual combinations showed bc-sensitivity/bc-specificity of > 90%: β-2-microglobulin with profilin-1, and lysozyme C with zymogen granule protein 16 homologue B.
New salivary biomarkers show good or excellent ability to diagnose periodontitis. Age has a more significant influence on the accuracy of the single biomarkers than smoking, with results comparable to two-protein combinations.
This narrative review aims to synthesize current knowledge on integrating single-cell genomics technologies with animal models of periodontitis and peri-implantitis.
Single-cell RNA sequencing (scRNAseq) reveals cellular heterogeneity and specific cell roles in periodontitis and peri-implantitis, overcoming the limitations of bulk RNA sequencing. Under controlled conditions and genetic manipulation, animal models facilitate studying disease progression, gene functions and systemic disease links, aiding targeted therapy development. Knockout models have started to elucidate the impact of genetic mutations on periodontal disease and host responses. scRNAseq in animal models has been used to examine connections between periodontitis and systemic diseases, revealing altered immune environments and cellular interactions. Emerging studies are now applying these methods to animal models of peri-implantitis. Integrating these datasets into single-cell and spatially resolved atlases will enable future meta-analyses, providing deeper insights into disease mechanisms considering factors such as sex, strain, and age.
Integrating scRNAseq with animal models advances the understanding of periodontitis and peri-implantitis pathogenesis and precision therapies. The combined use of single-cell and spatial genomics and scRNAseq will further enhance data insights significantly for drug discovery and preclinical testing, making these technologies pivotal in validating animal models and translating findings into clinical practice.
Aim: The objective of this cross-sectional survey was to assess the attitude among general practitioners (GPs) and periodontal specialists (PSs) in Norway towards developing and implementing guideline-based periodontal referral practise.
Material and methods: A multiple-choice questionnaire was distributed online to a sample of professionally active GPs and PSs. The survey included questions on demographics, practise profile, proficiency and insight among oral healthcare providers, periodontal referral patterns, and attitude on establishing guideline-based referral practise. Logistic regression analyses were employed to estimate associations between dependent and selected independent variables. Inverse probability weights (IPW) were applied to adjust for non-response in the logistic regression models.
Results: Analyses were based on answers from 353 GPs and 49 PSs. The majority of GPs (77.9%) considered periodontal referral guidelines as a useful tool if they were available, and 59.2% of PSs expressed a positive attitude for implementing guideline-based referral practise to prevent premature or delayed referrals. In total, 93.9% of PSs reported that patients with periodontitis were being referred from GPs too late either frequently or sometimes. Females tended to be more interested in utilising referral guidelines than males (odds ratio, OR = 2.89, p < 0.001). Older GPs and those with increasing years of practising, were less interested in using referral guidelines than younger and more inexperienced GPs (both p < 0.05).
Conclusions: The findings document a positive attitude and significant need for developing and implementing periodontal referral guidelines among GPs and PSs, ensuring timely identification and referral of patients with periodontitis.
Aim: To investigate the impact of different vertical interimplant distances (VIDs) on the long-term success of two adjacent single-unit implants.
Materials and methods: This retrospective study categorized implants into two groups based on VID: Group 1 (VID < 2 mm) and Group 2 (VID ≥ 2 mm). Further classification was done according to horizontal inter-implant distance (HID). Statistical models were used to evaluate the primary outcome of implant survival, secondary outcomes of complication-free survival rates and radiological findings.
Results: We included 290 patients with 376 two adjacent single-unit implants. Group 1 included 223 patients with 280 implants, whereas Group 2 comprised 89 patients with 96 implants. The implant survival rates were 93.2% in Group 1 and 96.8% in Group 2 over the 8 years, with no significant association between the groups. Similarly, the overall complication-free survival rates were 60.6% in Group 1 and 65.6% in Group 2, showing no significant association between the groups. Radiological assessments also showed no notable association between the groups. This trend was consistent in the subgroup analyses based on HID, with no significant differences in implant survival, complication-free survival rates or radiological outcomes.
Conclusions: Considering the study limitations of an 8-year post-prosthetic review period and despite varying VIDs, our findings showed no significant difference in implant survival, complication-free survival rates or radiological assessments. This underscores the positive prognosis of two adjacent single-unit implants and contributes to a deeper understanding of the success factors in implantology.
Trial registration: The study was registered in the Chinese Clinical Trial Registry (ChiCTR2300073215).
Aim: The aim of this study was to investigate the non-inferiority of alveolar ridge preservation (ARP) with membrane stabilization compared with non-stabilization, focusing on changes of soft-tissue contour in periodontally compromised extraction sockets. Secondary outcomes included changes in hard-tissue contour, patient-reported outcomes and new bone formation.
Materials and methods: Twenty-four patients with periodontally compromised teeth were randomly assigned to ARP with (test group) or without (control group) membrane stabilization. To assess profilometric and hard-tissue dimensional changes, dental impressions and cone beam computed tomography scans were performed at baseline (T0), immediately after ARP (T1) and 4 months post surgery (T2). Soft-tissue healing in open healing sites was evaluated at T2, and wound closure was assessed 10 days post surgery. Patient-reported outcomes were documented, and core biopsies were obtained for histomorphometric analysis.
Results: The absolute profilometric horizontal width change at 3 mm below the crest in the test group was not inferior to that in the control group. For the relative values, horizontal width reduction (3 and 5 mm below the crest) and volumetric shrinkage (3-5 mm below the crest) were lower in the test group. No significant differences were observed in bone dimensional changes, wound healing, pain and swelling or histomorphometric outcomes.
Conclusion: ARP with membrane stabilization in periodontally compromised extraction sockets is non-inferior in terms of soft-tissue contour changes to those without membrane fixation.
Trial registration: Clinical Research Information Service (CRIS), KCT0005280. Registered 4 August 2020, https://cris.nih.go.kr/cris/search/detailSearch.do?seq=19165&search_page=L.
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