John Rong Hao Tay, Birte Holtfreter, Sebastian-Edgar Baumeister, Marco A Peres, Gustavo G Nascimento
Aims: To compare periodontitis prevalence estimates based on the Application of the 2018 periodontal status Classification to Epidemiological Survey data (ACES) and the Centers for Disease Control and Prevention/American Academy of Periodontology (CDC/AAP) classification.
Materials and methods: National Health and Nutrition Examination Survey data for the years 2009/2010, 2011/2012 and 2013/2014 were survey-weighted and post-stratified to estimate the prevalence of periodontitis. Estimates based on ACES and CDC/AAP were cross-classified and stratified by age group. Prevalence estimates using different partial recording protocols were examined.
Results: Using the ACES framework, the prevalence of adults with periodontitis was 93.1% (95% CI: 91.9-94.2) (Stage I: 17.9%; Stage II: 46.2%; Stage III: 16.7%; Stage IV: 12.4%). Complexity factors did not alter Stage II prevalence. The CDC/AAP classification yielded a periodontitis prevalence of 38.9% (95% CI: 36.4-41.4) (Mild: 3.5%; Moderate: 28.1%; Severe: 7.3%). Partial recording protocols resulted in increased prevalence in the lower stages of periodontitis.
Conclusions: The European Federation of Periodontology/American Academy of Periodontology Classification (using the ACES framework) overestimates periodontitis cases compared with the CDC/AAP classification. Including complexity factors in the ACES framework provides limited benefits in staging periodontitis. Partial-mouth recording protocols overestimate health and early disease stages while underestimating more severe disease.
{"title":"Application of the 2018 Periodontal Status Classification to Epidemiological Survey Data (ACES) Framework to Estimate the Periodontitis Prevalence in the United States.","authors":"John Rong Hao Tay, Birte Holtfreter, Sebastian-Edgar Baumeister, Marco A Peres, Gustavo G Nascimento","doi":"10.1111/jcpe.14132","DOIUrl":"https://doi.org/10.1111/jcpe.14132","url":null,"abstract":"<p><strong>Aims: </strong>To compare periodontitis prevalence estimates based on the Application of the 2018 periodontal status Classification to Epidemiological Survey data (ACES) and the Centers for Disease Control and Prevention/American Academy of Periodontology (CDC/AAP) classification.</p><p><strong>Materials and methods: </strong>National Health and Nutrition Examination Survey data for the years 2009/2010, 2011/2012 and 2013/2014 were survey-weighted and post-stratified to estimate the prevalence of periodontitis. Estimates based on ACES and CDC/AAP were cross-classified and stratified by age group. Prevalence estimates using different partial recording protocols were examined.</p><p><strong>Results: </strong>Using the ACES framework, the prevalence of adults with periodontitis was 93.1% (95% CI: 91.9-94.2) (Stage I: 17.9%; Stage II: 46.2%; Stage III: 16.7%; Stage IV: 12.4%). Complexity factors did not alter Stage II prevalence. The CDC/AAP classification yielded a periodontitis prevalence of 38.9% (95% CI: 36.4-41.4) (Mild: 3.5%; Moderate: 28.1%; Severe: 7.3%). Partial recording protocols resulted in increased prevalence in the lower stages of periodontitis.</p><p><strong>Conclusions: </strong>The European Federation of Periodontology/American Academy of Periodontology Classification (using the ACES framework) overestimates periodontitis cases compared with the CDC/AAP classification. Including complexity factors in the ACES framework provides limited benefits in staging periodontitis. Partial-mouth recording protocols overestimate health and early disease stages while underestimating more severe disease.</p>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hager R Zein Elabdeen, Ellen Berggreen, Knut Helge Jensen, Gerhard Sulo, Siri Flagestad Kvalheim, Linda C Hasselgren, Eva Gerdts, Marjolein Memelink Iversen, Anne Isine Bolstad, Dagmar F Bunæs
Aim: To assess the prevalence and severity of periodontitis in an older adult population in Western Norway using the periodontitis definition by the recently published ACES 2018 Classification Framework.
Materials and methods: In the present study, 1298 70-year-old subjects answered a questionnaire and underwent a full-mouth periodontal examination. The prevalence of periodontitis, stages, extents and grades was presented according to the Framework applying the 2018 periodontal classification scheme in epidemiological survey (ACES). Descriptive analyses were used, and results were expressed as percentages of the total number of observations.
Results: According to ACES, periodontitis was detected in 100% of the study population, and Stage II, Stage III and Stage IV were present in 21%, 75% and 4% of the individuals, respectively. The majority of periodontitis cases demonstrated generalised periodontitis and Grade B was the predominant grade in the periodontitis cases. The mean number of teeth in the study population was 25.3.
Conclusion: There is a high burden of periodontitis in the present population of old individuals, and the majority of participants were classified as Stage III periodontitis. The cut-offs for interdental clinical attachment loss in the definition of periodontitis affect the estimate of cases, stages and extent of periodontitis in older age groups.
{"title":"Prevalence of Periodontitis in a 70-Year-Old Population in Western Norway According to the ACES 2018 Classification Framework: A Cross-Sectional Study.","authors":"Hager R Zein Elabdeen, Ellen Berggreen, Knut Helge Jensen, Gerhard Sulo, Siri Flagestad Kvalheim, Linda C Hasselgren, Eva Gerdts, Marjolein Memelink Iversen, Anne Isine Bolstad, Dagmar F Bunæs","doi":"10.1111/jcpe.14128","DOIUrl":"https://doi.org/10.1111/jcpe.14128","url":null,"abstract":"<p><strong>Aim: </strong>To assess the prevalence and severity of periodontitis in an older adult population in Western Norway using the periodontitis definition by the recently published ACES 2018 Classification Framework.</p><p><strong>Materials and methods: </strong>In the present study, 1298 70-year-old subjects answered a questionnaire and underwent a full-mouth periodontal examination. The prevalence of periodontitis, stages, extents and grades was presented according to the Framework applying the 2018 periodontal classification scheme in epidemiological survey (ACES). Descriptive analyses were used, and results were expressed as percentages of the total number of observations.</p><p><strong>Results: </strong>According to ACES, periodontitis was detected in 100% of the study population, and Stage II, Stage III and Stage IV were present in 21%, 75% and 4% of the individuals, respectively. The majority of periodontitis cases demonstrated generalised periodontitis and Grade B was the predominant grade in the periodontitis cases. The mean number of teeth in the study population was 25.3.</p><p><strong>Conclusion: </strong>There is a high burden of periodontitis in the present population of old individuals, and the majority of participants were classified as Stage III periodontitis. The cut-offs for interdental clinical attachment loss in the definition of periodontitis affect the estimate of cases, stages and extent of periodontitis in older age groups.</p>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stefan P. Bienz, Jasmine Kuonen, Jürg Hüsler, Naida Gadzo, Sonja Hitz, Ronald E. Jung, Daniel S. Thoma
AimTo compare the histological structure of biopsies from the anterior and posterior regions of the human palate in order to gain insights into potential differences of soft tissue harvesting sites.MethodsThis comparative study involved 20 participants, from each of whom four samples from the palatal mucosa were collected. The biopsies were histologically processed and subsequently analysed. The thickness of the epithelium, lamina propria, and the length of the rete pegs were measured using a microscope. In addition, parameters such as collagen fibres, elastic fibres, elastic connective tissue, loose connective tissue and the background were measured.ResultsThe mean epithelial thickness was 0.42 mm anteriorly and 0.37 mm posteriorly. The mean thickness of the lamina propria was 0.81 mm anteriorly and 0.85 mm posteriorly. Within the region of interest, collagen fibres were significantly less abundant anteriorly than posteriorly (p = 0.016). The loose connective tissue showed a significantly higher occurrence in the anterior compared with the posterior sampling site (p = 0.004).ConclusionThis trial indicates no differences in terms of the thickness of the lamina propria. There was a higher concentration of collagen fibres and a lower concentration of loose connective tissue in the posterior region of the palate.
{"title":"Comparison of the Histological Structure of an Anterior as well as a Posterior Biopsy From the Human Palate: A Comparative Study","authors":"Stefan P. Bienz, Jasmine Kuonen, Jürg Hüsler, Naida Gadzo, Sonja Hitz, Ronald E. Jung, Daniel S. Thoma","doi":"10.1111/jcpe.14133","DOIUrl":"https://doi.org/10.1111/jcpe.14133","url":null,"abstract":"AimTo compare the histological structure of biopsies from the anterior and posterior regions of the human palate in order to gain insights into potential differences of soft tissue harvesting sites.MethodsThis comparative study involved 20 participants, from each of whom four samples from the palatal mucosa were collected. The biopsies were histologically processed and subsequently analysed. The thickness of the epithelium, lamina propria, and the length of the rete pegs were measured using a microscope. In addition, parameters such as collagen fibres, elastic fibres, elastic connective tissue, loose connective tissue and the background were measured.ResultsThe mean epithelial thickness was 0.42 mm anteriorly and 0.37 mm posteriorly. The mean thickness of the lamina propria was 0.81 mm anteriorly and 0.85 mm posteriorly. Within the region of interest, collagen fibres were significantly less abundant anteriorly than posteriorly (<jats:italic>p</jats:italic> = 0.016). The loose connective tissue showed a significantly higher occurrence in the anterior compared with the posterior sampling site (<jats:italic>p</jats:italic> = 0.004).ConclusionThis trial indicates no differences in terms of the thickness of the lamina propria. There was a higher concentration of collagen fibres and a lower concentration of loose connective tissue in the posterior region of the palate.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"60 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143056326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marcela Mayana Pereira Franco, Cecilia Claudia Costa Ribeiro, Lorena Lúcia Costa Ladeira, Mario Augusto Brondani, Erika Bárbara Abreu Fonseca Thomaz, Cláudia Maria Coelho Alves
AimTo investigate the association between molar incisor hypomineralization (MIH) and periodontitis in adolescents.MethodsA population‐based study was undertaken of Brazilian adolescents aged 18 and 19 years (n = 2515). MIH was assessed according to Ghanim's criteria. The outcomes were Periodontitis Indicators, a continuous latent variable estimated on the basis of the common variance shared by the indicators bleeding on probing (BoP), probing pocket depth (PPD ≥ 4 mm) and clinical attachment level (CAL ≥ 3 mm), as well as periodontitis cases defined according to CDC‐AAP. Structural equation modelling was used, and the models were adjusted for lower socioeconomic status, sex, smoking, triglycerides/high‐density lipoprotein (TG/HDL) (mg/dL) and visible plaque index. Two other approaches were used for sensitivity analysis: (i) logistic regression, considering the effect of MIH on periodontitis (CDC/AAP) at the individual level, and (ii) multilevel regression to evaluate the association of MIH with CAL and PPD, considering the tooth as the unit of analysis, adjusted for other tooth‐level and individual‐level variables.ResultsThe prevalence of MIH was 16.86% (n = 423). Individuals with MIH had higher values of the Periodontitis Indicators through a direct pathway (standardized coefficient [SC] = 0.193, p < 0.001) and indirectly mediated by biofilm (SC = 0.263, p < 0.001). MIH was also associated with periodontitis according to CDC‐AAP (SC = 0.071, p < 0.001) and indirectly mediated by biofilm (SC = 0.344, p < 0.001). Consistently, lower socioeconomic status, sex, TG/HDL and biofilm were associated with both periodontal outcomes. Tooth‐level sensitivity regression analysis confirmed the association observed in individual‐level analyses.ConclusionOur findings suggest that adolescents with MIH are susceptible to periodontitis, and it is therefore important to monitor their periodontal health.
目的探讨青少年磨牙切牙低矿化（MIH）与牙周炎的关系。方法对18岁和19岁的巴西青少年进行了一项基于人群的研究（n = 2515）。根据加尼姆的标准对MIH进行评估。结果是牙周炎指标，这是一个连续的潜在变量，根据探针出血指标（BoP）、探针口袋深度（PPD≥4 mm）和临床附着水平（CAL≥3 mm）以及根据CDC - AAP定义的牙周炎病例所共有的共同方差进行估计。使用结构方程模型，并根据较低的社会经济地位、性别、吸烟、甘油三酯/高密度脂蛋白（TG/HDL） （mg/dL）和可见斑块指数对模型进行了调整。另外两种方法用于敏感性分析：(i)逻辑回归，考虑MIH对牙周炎（CDC/AAP）在个体水平的影响；（ii）多水平回归，评估MIH与CAL和PPD的关系，考虑牙齿作为分析单位，调整其他牙齿水平和个体水平变量。结果mh患病率为16.86% （n = 423）。通过直接途径，MIH患者的牙周炎指标较高(标准化系数[SC] = 0.193, p <；0.001)和间接介导的生物膜(SC = 0.263, p <；0.001)。根据CDC - AAP， MIH也与牙周炎相关(SC = 0.071, p <；0.001)和间接介导的生物膜(SC = 0.344, p <；0.001)。同样，较低的社会经济地位、性别、TG/HDL和生物膜与两种牙周结局有关。牙齿水平敏感性回归分析证实了个体水平分析中观察到的相关性。结论青少年MIH患者易患牙周炎，应加强对其牙周健康的监测。
{"title":"Molar Incisor Hypomineralization and Periodontitis in Adolescents: A Population‐Based Study","authors":"Marcela Mayana Pereira Franco, Cecilia Claudia Costa Ribeiro, Lorena Lúcia Costa Ladeira, Mario Augusto Brondani, Erika Bárbara Abreu Fonseca Thomaz, Cláudia Maria Coelho Alves","doi":"10.1111/jcpe.14123","DOIUrl":"https://doi.org/10.1111/jcpe.14123","url":null,"abstract":"AimTo investigate the association between molar incisor hypomineralization (MIH) and periodontitis in adolescents.MethodsA population‐based study was undertaken of Brazilian adolescents aged 18 and 19 years (<jats:italic>n</jats:italic> = 2515). MIH was assessed according to Ghanim's criteria. The outcomes were Periodontitis Indicators, a continuous latent variable estimated on the basis of the common variance shared by the indicators bleeding on probing (BoP), probing pocket depth (PPD ≥ 4 mm) and clinical attachment level (CAL ≥ 3 mm), as well as periodontitis cases defined according to CDC‐AAP. Structural equation modelling was used, and the models were adjusted for lower socioeconomic status, sex, smoking, triglycerides/high‐density lipoprotein (TG/HDL) (mg/dL) and visible plaque index. Two other approaches were used for sensitivity analysis: (i) logistic regression, considering the effect of MIH on periodontitis (CDC/AAP) at the individual level, and (ii) multilevel regression to evaluate the association of MIH with CAL and PPD, considering the tooth as the unit of analysis, adjusted for other tooth‐level and individual‐level variables.ResultsThe prevalence of MIH was 16.86% (<jats:italic>n</jats:italic> = 423). Individuals with MIH had higher values of the Periodontitis Indicators through a direct pathway (standardized coefficient [SC] = 0.193, <jats:italic>p</jats:italic> < 0.001) and indirectly mediated by biofilm (SC = 0.263, <jats:italic>p</jats:italic> < 0.001). MIH was also associated with periodontitis according to CDC‐AAP (SC = 0.071, <jats:italic>p</jats:italic> < 0.001) and indirectly mediated by biofilm (SC = 0.344, <jats:italic>p</jats:italic> < 0.001). Consistently, lower socioeconomic status, sex, TG/HDL and biofilm were associated with both periodontal outcomes. Tooth‐level sensitivity regression analysis confirmed the association observed in individual‐level analyses.ConclusionOur findings suggest that adolescents with MIH are susceptible to periodontitis, and it is therefore important to monitor their periodontal health.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"84 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AimTo investigate the involvement of low‐density lipoprotein receptor‐related protein 5 (LRP5) in inflammation and alveolar bone loss in periodontitis.Materials and MethodsGingival tissues were obtained from 10 periodontitis patients and 10 healthy individuals. Wild‐type (WT) and osteoblast‐specific Lrp5 conditional knock‐out C57BL/6 (LRP5fl/fl;Oc‐Cre) mice were used to establish a ligature‐induced mouse model of periodontitis. Human periodontal ligament stem cells (hPDLSCs) were isolated and used to further verify the mechanism through which LRP5 mediates periodontitis in vitro. Micro‐computed tomography, haematoxylin and eosin staining, immunohistochemistry, quantitative reverse‐transcription PCR, western blotting, enzyme‐linked immunosorbent assay and RNA sequencing were performed to explore the role of LRP5 in periodontitis and the underlying mechanism.ResultsLRP5 expression was down‐regulated in human/mouse periodontal tissues compared to that in healthy controls. Compared to those in wild‐type mice, the periodontal tissues of LRP5fl/fl;Oc‐Cre mice had increased alveolar bone loss, higher proinflammatory cytokine levels, and lower osteogenesis‐related factor expression. LRP5 expression was down‐regulated in hPDLSCs after lipopolysaccharide treatment in vitro. LRP5 knockdown increased proinflammatory cytokine production and inhibited osteoblastogenesis by inhibiting PI3K/c‐FOS signalling.ConclusionLRP5 down‐regulation exacerbates inflammation and alveolar bone loss in periodontitis by inhibiting PI3K/c‐FOS signalling, suggesting LRP5 as a potential therapeutic target for periodontitis.
{"title":"LRP5 Down‐Regulation Exacerbates Inflammation and Alveolar Bone Loss in Periodontitis by Inhibiting PI3K/c‐FOS Signalling","authors":"Hui Jiang, Yue Xi, Qifeng Jiang, Wei Dai, Xiaoru Qin, Jing Zhang, Zhiwei Jiang, Guoli Yang, Qianming Chen","doi":"10.1111/jcpe.14112","DOIUrl":"https://doi.org/10.1111/jcpe.14112","url":null,"abstract":"AimTo investigate the involvement of low‐density lipoprotein receptor‐related protein 5 (LRP5) in inflammation and alveolar bone loss in periodontitis.Materials and MethodsGingival tissues were obtained from 10 periodontitis patients and 10 healthy individuals. Wild‐type (WT) and osteoblast‐specific Lrp5 conditional knock‐out C57BL/6 (LRP5fl/fl;Oc‐Cre) mice were used to establish a ligature‐induced mouse model of periodontitis. Human periodontal ligament stem cells (hPDLSCs) were isolated and used to further verify the mechanism through which LRP5 mediates periodontitis in vitro. Micro‐computed tomography, haematoxylin and eosin staining, immunohistochemistry, quantitative reverse‐transcription PCR, western blotting, enzyme‐linked immunosorbent assay and RNA sequencing were performed to explore the role of LRP5 in periodontitis and the underlying mechanism.ResultsLRP5 expression was down‐regulated in human/mouse periodontal tissues compared to that in healthy controls. Compared to those in wild‐type mice, the periodontal tissues of LRP5fl/fl;Oc‐Cre mice had increased alveolar bone loss, higher proinflammatory cytokine levels, and lower osteogenesis‐related factor expression. LRP5 expression was down‐regulated in hPDLSCs after lipopolysaccharide treatment in vitro. LRP5 knockdown increased proinflammatory cytokine production and inhibited osteoblastogenesis by inhibiting PI3K/c‐FOS signalling.ConclusionLRP5 down‐regulation exacerbates inflammation and alveolar bone loss in periodontitis by inhibiting PI3K/c‐FOS signalling, suggesting LRP5 as a potential therapeutic target for periodontitis.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"24 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142992106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zi‐an Tong, Long Chen, Ling Shen, Yi‐fan Lu, Jian‐wei Zhang, Ya‐dong Qi, Yin‐jie Qian, Si‐qi Bao, Wei Chen, Mi‐si Si
AimTo propose a modified method for establishing a peri‐implantitis murine model, assess the gene expression profile and immune cell infiltration of the gingiva and alveolar bone, and evaluate the transcriptomic similarity between patients with peri‐implantitis and the corresponding murine model.Materials and MethodsA ligature‐induced peri‐implantitis murine model was established using an immediate implant placement approach. RNA sequencing was performed to determine the transcriptomic profiles of peri‐implant tissues from mice, patients with peri‐implantitis and healthy individuals. Histopathological and bioinformatics analyses were performed to measure immune cell infiltration, bone remodelling, and inflammatory reactions.ResultsMouse gingival tissue showed strong immune and inflammatory responses, especially macrophage functions; these responses were weaker in the alveolar bone. Humans and mice showed similar gene expression patterns in the gingiva, with greater infiltration of macrophages and neutrophils.ConclusionsDuring peri‐implantitis progression, mouse gingival tissue exhibited increased immune‐related functions and inflammation compared with the alveolar bone. Patients with peri‐implantitis and the murine model displayed transcriptomic similarities within the gingiva. We propose that the modified ligature‐induced peri‐implantitis murine model is suitable for investigating peri‐implantitis pathogenesis, with macrophages and neutrophils potentially being critical in its development.
{"title":"A Modified Ligature‐Induced Peri‐Implantitis Murine Model and RNA Sequencing Analysis Compared With Human Subjects","authors":"Zi‐an Tong, Long Chen, Ling Shen, Yi‐fan Lu, Jian‐wei Zhang, Ya‐dong Qi, Yin‐jie Qian, Si‐qi Bao, Wei Chen, Mi‐si Si","doi":"10.1111/jcpe.14127","DOIUrl":"https://doi.org/10.1111/jcpe.14127","url":null,"abstract":"AimTo propose a modified method for establishing a peri‐implantitis murine model, assess the gene expression profile and immune cell infiltration of the gingiva and alveolar bone, and evaluate the transcriptomic similarity between patients with peri‐implantitis and the corresponding murine model.Materials and MethodsA ligature‐induced peri‐implantitis murine model was established using an immediate implant placement approach. RNA sequencing was performed to determine the transcriptomic profiles of peri‐implant tissues from mice, patients with peri‐implantitis and healthy individuals. Histopathological and bioinformatics analyses were performed to measure immune cell infiltration, bone remodelling, and inflammatory reactions.ResultsMouse gingival tissue showed strong immune and inflammatory responses, especially macrophage functions; these responses were weaker in the alveolar bone. Humans and mice showed similar gene expression patterns in the gingiva, with greater infiltration of macrophages and neutrophils.ConclusionsDuring peri‐implantitis progression, mouse gingival tissue exhibited increased immune‐related functions and inflammation compared with the alveolar bone. Patients with peri‐implantitis and the murine model displayed transcriptomic similarities within the gingiva. We propose that the modified ligature‐induced peri‐implantitis murine model is suitable for investigating peri‐implantitis pathogenesis, with macrophages and neutrophils potentially being critical in its development.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"45 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. M. Nguyen, A. V. Tran, J. P. Kincheloe, J. E. Ebersole
IntroductionTelomeres are nucleotide sequences found at the end of chromosomes, and their shortening is associated with chronological and biological ageing, oxidative stress and malnutrition. Shorter telomeres have been shown to be associated with periodontitis. Dietary nutrients are also influential factors in the aetiology and progression of periodontitis and other chronic inflammatory diseases. We tested the hypothesis that blood serum levels of folate, vitamin D, vitamin E, cis‐β‐carotene and/or β‐cryptoxanthin are associated with telomere lengths and periodontitis and that these relationships are modified by factors that also affect periodontitis (e.g., age, sex, smoking, race/ethnicity).MethodsLaboratory and demographic data collected by the NHANES 1999–2002 survey of 10,793 study participants were analysed. The data included age (binned every 10 years starting at age 30), race/ethnicity (White, Black, Hispanic), sex (male, female), periodontitis (none/mild, moderate/severe), vitamin levels (low, medium, high), telomere lengths (base pairs) and smoking (yes, no). Statistical analyses were performed with ANOVA tests.ResultsPeriodontitis was significantly associated with vitamin D, vitamin E and cis‐β‐carotene. Telomere length was significantly associated with vitamin E and cis‐β‐carotene. There were statistically significant interactions between the following vitamins and covariates on telomere length: folate and sex, vitamin D and periodontitis, vitamin D and race/ethnicity and vitamin E and sex.ConclusionsCertain blood nutrients may disrupt biological ageing, with periodontitis as a co‐morbidity. Some of these relationships are modified by demographic variables such as sex and race/ethnicity.
导言端粒是染色体末端的核苷酸序列，端粒的缩短与时间和生物老化、氧化应激和营养不良有关。端粒的缩短已被证明与牙周炎有关。膳食营养素也是牙周炎和其他慢性炎症性疾病的病因和进展的影响因素。我们测试了这样一个假设：血清中叶酸、维生素 D、维生素 E、顺式-β-胡萝卜素和/或 β-隐黄素的水平与端粒长度和牙周炎有关，而且这些关系会因同样影响牙周炎的因素（如年龄、性别、吸烟、种族/民族）而改变。数据包括年龄（从 30 岁开始，每 10 年为一档）、种族/民族（白人、黑人、西班牙裔）、性别（男性、女性）、牙周炎（无/轻度、中度/重度）、维生素水平（低、中、高）、端粒长度（碱基对）和吸烟（是、否）。结果 牙周炎与维生素 D、维生素 E 和顺式-β-胡萝卜素显著相关。端粒长度与维生素 E 和顺式-β-胡萝卜素明显相关。以下维生素与端粒长度的协变量之间存在统计学意义上的交互作用：叶酸与性别、维生素 D 与牙周炎、维生素 D 与种族/民族、维生素 E 与性别。其中一些关系会因人口统计学变量（如性别和种族/民族）而改变。
{"title":"Serum Nutrients, Periodontitis and Biological Ageing","authors":"L. M. Nguyen, A. V. Tran, J. P. Kincheloe, J. E. Ebersole","doi":"10.1111/jcpe.14125","DOIUrl":"https://doi.org/10.1111/jcpe.14125","url":null,"abstract":"IntroductionTelomeres are nucleotide sequences found at the end of chromosomes, and their shortening is associated with chronological and biological ageing, oxidative stress and malnutrition. Shorter telomeres have been shown to be associated with periodontitis. Dietary nutrients are also influential factors in the aetiology and progression of periodontitis and other chronic inflammatory diseases. We tested the hypothesis that blood serum levels of folate, vitamin D, vitamin E, <jats:italic>cis</jats:italic>‐β‐carotene and/or β‐cryptoxanthin are associated with telomere lengths and periodontitis and that these relationships are modified by factors that also affect periodontitis (e.g., age, sex, smoking, race/ethnicity).MethodsLaboratory and demographic data collected by the NHANES 1999–2002 survey of 10,793 study participants were analysed. The data included age (binned every 10 years starting at age 30), race/ethnicity (White, Black, Hispanic), sex (male, female), periodontitis (none/mild, moderate/severe), vitamin levels (low, medium, high), telomere lengths (base pairs) and smoking (yes, no). Statistical analyses were performed with ANOVA tests.ResultsPeriodontitis was significantly associated with vitamin D, vitamin E and <jats:italic>cis</jats:italic>‐β‐carotene. Telomere length was significantly associated with vitamin E and <jats:italic>cis</jats:italic>‐β‐carotene. There were statistically significant interactions between the following vitamins and covariates on telomere length: folate and sex, vitamin D and periodontitis, vitamin D and race/ethnicity and vitamin E and sex.ConclusionsCertain blood nutrients may disrupt biological ageing, with periodontitis as a co‐morbidity. Some of these relationships are modified by demographic variables such as sex and race/ethnicity.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"75 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuan Li, Julie Choi Ka Kung, Junyu Shi, Xinyu Wu, Steve Lut Ting Lam, Ke Deng, Xiao Zhang, Hongchang Lai, George Pelekos, Lijian Jin, Maurizio S Tonetti
Aim: To evaluate the diagnostic accuracy of an active matrix metalloproteinase-8 (aMMP-8) point-of-care oral rinse test (POC-ORT) for predicting periodontitis in treatment-naïve subjects in two independent studies and update a recent meta-analysis.
Methods: The aMMP-8 POC-ORT index test was performed in a representative population in Hong Kong, China, and a consecutive convenience sample in Shanghai, China. The reference standard was the 2017 World Workshop classification of periodontal diseases. Sensitivity, specificity, and the area under the receiver operating characteristic (AUROC) curve were assessed. The original data were used to update a recent Bayesian meta-analysis following the current Cochrane guideline for diagnostic trials. The GRADE framework was used to interpret the strength and certainty of the evidence.
Results: Three-hundred and eighty-four and 390 subjects were enrolled in the Hong Kong and Shanghai studies, respectively; 74.5% and 67.2% had periodontitis. An aMMP-8-positive test predicted periodontitis with an AUROC of 0.661 and 0.669 in the two studies. The updated systematic review and meta-analysis included eight studies and 2048 subjects. After considering the risk of bias, indirectness, inconsistency, imprecision and publication bias, it showed moderate certainty of a sensitivity of 0.59 (95% CrI: 0.42-0.75), a specificity of 0.82 (95% CrI: 0.68-0.93) and a hierarchical summary AUROC of 0.77 (95% CrI: 0.74-0.81).
Conclusion: There is moderate certainty that the aMMP-8 POC-ORT test predicts periodontitis with low to moderate sensitivity, moderate to high specificity, and moderate accuracy. Its high false-negative rate does not allow the replacement of clinical examinations when available. The moderate to high positive predictive value shows the potential utility of a positive test for self-detection or co-management of periodontitis in a medical setting and its incorporation in multi-test diagnostics. Further investigations are highly warranted.
{"title":"Diagnostic Accuracy of a Point-Of-Care aMMP-8 Test for Discriminating Periodontal Health Status in Adults: Validation Trials and Updated Meta-Analysis.","authors":"Yuan Li, Julie Choi Ka Kung, Junyu Shi, Xinyu Wu, Steve Lut Ting Lam, Ke Deng, Xiao Zhang, Hongchang Lai, George Pelekos, Lijian Jin, Maurizio S Tonetti","doi":"10.1111/jcpe.14119","DOIUrl":"https://doi.org/10.1111/jcpe.14119","url":null,"abstract":"<p><strong>Aim: </strong>To evaluate the diagnostic accuracy of an active matrix metalloproteinase-8 (aMMP-8) point-of-care oral rinse test (POC-ORT) for predicting periodontitis in treatment-naïve subjects in two independent studies and update a recent meta-analysis.</p><p><strong>Methods: </strong>The aMMP-8 POC-ORT index test was performed in a representative population in Hong Kong, China, and a consecutive convenience sample in Shanghai, China. The reference standard was the 2017 World Workshop classification of periodontal diseases. Sensitivity, specificity, and the area under the receiver operating characteristic (AUROC) curve were assessed. The original data were used to update a recent Bayesian meta-analysis following the current Cochrane guideline for diagnostic trials. The GRADE framework was used to interpret the strength and certainty of the evidence.</p><p><strong>Results: </strong>Three-hundred and eighty-four and 390 subjects were enrolled in the Hong Kong and Shanghai studies, respectively; 74.5% and 67.2% had periodontitis. An aMMP-8-positive test predicted periodontitis with an AUROC of 0.661 and 0.669 in the two studies. The updated systematic review and meta-analysis included eight studies and 2048 subjects. After considering the risk of bias, indirectness, inconsistency, imprecision and publication bias, it showed moderate certainty of a sensitivity of 0.59 (95% CrI: 0.42-0.75), a specificity of 0.82 (95% CrI: 0.68-0.93) and a hierarchical summary AUROC of 0.77 (95% CrI: 0.74-0.81).</p><p><strong>Conclusion: </strong>There is moderate certainty that the aMMP-8 POC-ORT test predicts periodontitis with low to moderate sensitivity, moderate to high specificity, and moderate accuracy. Its high false-negative rate does not allow the replacement of clinical examinations when available. The moderate to high positive predictive value shows the potential utility of a positive test for self-detection or co-management of periodontitis in a medical setting and its incorporation in multi-test diagnostics. Further investigations are highly warranted.</p>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":" ","pages":""},"PeriodicalIF":5.8,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mingyan Xu, Feixiang Zhu, Yifan Guo, Fan Liu, Songlin Shi, Ling Yang, Rui Huang, Xiaoling Deng
AimsCircular RNA forkhead box O3 (circFOXO3) is crucial in regulating inflammation in lung and heart injuries. However, its role in periodontitis remains unclear. We sought to elucidate the effects of circFOXO3 on periodontitis progression and related molecular mechanisms.MethodsReverse‐transcription quantitative polymerase chain reaction and fluorescence in situ hybridization were used to quantify and localize circFOXO3 expression. The mechanism by which circFOXO3 promotes inflammation in periodontitis was investigated using epithelial cells, human gingival epithelium and a rat model of ligature‐induced periodontitis.ResultscircFOXO3 expression was abnormally high in the gingival epithelial tissues of patients with periodontitis. Elevated circFOXO3 levels down‐regulated microRNA (miR)‐141‐3p, leading to increased FOXO3 expression. FOXO3 interacted with JunB to form a transcriptional‐repression complex that inhibited the integrin β6 (ITGβ6)‐mediated activation of transforming growth factor β (TGF‐β) in epithelial cells. Through the miR‐141‐3p/FOXO3/JunB axis, circFOXO3 suppressed TGF‐β signalling, thereby exacerbating periodontal inflammation. Finally, circFOXO3 inhibition hindered disease progression and restored TGF‐β activity in vivo via the FOXO3/JunB/ITGβ6 pathway.ConclusionOur study identified a novel mechanism by which circFOXO3 contributes to periodontal inflammation through a complex transcriptional regulatory network involving miR‐141‐3p, FOXO3, JunB and ITGβ6. These findings highlight potential therapeutic targets for the development of effective treatments for this debilitating disease.
{"title":"Targeting circFOXO3 to Modulate Integrin β6 Expression in Periodontitis: A Potential Therapeutic Approach","authors":"Mingyan Xu, Feixiang Zhu, Yifan Guo, Fan Liu, Songlin Shi, Ling Yang, Rui Huang, Xiaoling Deng","doi":"10.1111/jcpe.14124","DOIUrl":"https://doi.org/10.1111/jcpe.14124","url":null,"abstract":"AimsCircular RNA forkhead box O3 (circFOXO3) is crucial in regulating inflammation in lung and heart injuries. However, its role in periodontitis remains unclear. We sought to elucidate the effects of circFOXO3 on periodontitis progression and related molecular mechanisms.MethodsReverse‐transcription quantitative polymerase chain reaction and fluorescence in situ hybridization were used to quantify and localize circFOXO3 expression. The mechanism by which circFOXO3 promotes inflammation in periodontitis was investigated using epithelial cells, human gingival epithelium and a rat model of ligature‐induced periodontitis.ResultscircFOXO3 expression was abnormally high in the gingival epithelial tissues of patients with periodontitis. Elevated circFOXO3 levels down‐regulated microRNA (miR)‐141‐3p, leading to increased FOXO3 expression. FOXO3 interacted with JunB to form a transcriptional‐repression complex that inhibited the integrin β6 (ITGβ6)‐mediated activation of transforming growth factor β (TGF‐β) in epithelial cells. Through the miR‐141‐3p/FOXO3/JunB axis, circFOXO3 suppressed TGF‐β signalling, thereby exacerbating periodontal inflammation. Finally, circFOXO3 inhibition hindered disease progression and restored TGF‐β activity in vivo via the FOXO3/JunB/ITGβ6 pathway.ConclusionOur study identified a novel mechanism by which circFOXO3 contributes to periodontal inflammation through a complex transcriptional regulatory network involving miR‐141‐3p, FOXO3, JunB and ITGβ6. These findings highlight potential therapeutic targets for the development of effective treatments for this debilitating disease.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"14 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mi Du, Ke Deng, Jiawei Yin, Chenkai Wu, Shixian Hu, Linxu Guo, Zhixin Luo, Maurizio Tonetti, Geerten‐Has E. Tjakkes, Anita Visser, Shaohua Ge, An Li
AimMasticatory dysfunction due to tooth loss is a potentially modifiable risk for mortality, but the pathway behind that remains to be investigated. This prospective study aimed to examine the role of diet and ageing in the associations between chewing capacity and long‐term mortality.MethodsData were obtained from participants (aged ≥ 20) in the National Health Nutritional and Health Survey (NHANES 1999–2010, n = 22,900). The mortality follow‐up ended on 31 December 2019. Chewing capacity was determined by the number of functional tooth units (FTUs). Diet information in NHANES was collected using a 24‐h‐recall questionnaire, and diet quality was measured by three index‐based dietary patterns, namely the nutrition index (NI), energy‐adjusted dietary inflammatory index (E‐DII) and healthy eating index‐2015 (HEI‐2015). The biological ageing process was reflected using phenotypic age acceleration (PhenoAgeAccel) and frailty index. Mediation analyses were conducted to assess the role of diet quality in the association between FTUs and ageing, as well as the role of ageing in the association between impaired chewing capacity (ICC) and mortality.ResultsParticipants with more FTUs were found to have a slower biological ageing process. Diet quality scores were estimated to mediate 9.0%–23.0% of the association between chewing capacity and biological ageing. Multivariable Cox proportional hazards regression models found a positive association between ICC and all‐cause mortality (hazard ratio = 1.282 [95% confidence interval: 1.189–1.382]). Also, ICC was significantly associated with a 28.9% higher risk of mortality due to cardiovascular disease (CVD) and a 32.7% higher risk of mortality due to cancer. Mediation analyses indicated that PhenoAgeAccel mediated the effect of ICC on all‐cause, CVD and cancer mortality with proportions of 18.1%, 17.3% and 12.5%, respectively. Similar mediating proportions were observed in the frailty index (range: 11.6%–23.5%).ConclusionsICC was associated with poorer diet quality and accelerated ageing, resulting in higher mortality risk. Therefore, it is plausible that dietary interventions and oral rehabilitation would promote healthy longevity, although further investigations are needed.
目的牙齿脱落引起的咀嚼功能障碍是一种潜在的可改变的死亡风险，但其背后的途径仍有待研究。这项前瞻性研究旨在研究饮食和衰老在咀嚼能力和长期死亡率之间的关系中的作用。方法数据来自国家健康营养与健康调查（NHANES 1999-2010, n = 22,900）参与者（年龄≥20岁）。死亡率随访于2019年12月31日结束。咀嚼能力由功能牙单元（ftu）的数量决定。采用24小时召回问卷收集NHANES中的饮食信息，并通过三种基于指数的饮食模式（即营养指数（NI）、能量调节饮食炎症指数（E‐DII）和健康饮食指数- 2015 (HEI‐2015)）来衡量饮食质量。利用表型年龄加速（PhenoAgeAccel）和脆弱指数反映生物衰老过程。进行了中介分析，以评估饮食质量在ftu和衰老之间的关联中的作用，以及衰老在咀嚼能力受损（ICC）和死亡率之间的关联中的作用。结果ftu越多的参与者生物衰老过程越慢。据估计，饮食质量评分介导了咀嚼能力和生物衰老之间9.0%-23.0%的关联。多变量Cox比例风险回归模型发现ICC与全因死亡率呈正相关（风险比= 1.282[95%置信区间：1.189-1.382]）。此外，ICC与心血管疾病（CVD）死亡率增加28.9%和癌症死亡率增加32.7%显著相关。中介分析表明，PhenoAgeAccel介导ICC对全因、CVD和癌症死亡率的影响分别为18.1%、17.3%和12.5%。在脆弱指数中也观察到类似的中介比例（范围：11.6%-23.5%）。结论sicc与饮食质量差、衰老加速有关，死亡风险较高。因此，饮食干预和口腔康复可能会促进健康长寿，尽管还需要进一步的研究。
{"title":"Association Between Chewing Capacity and Mortality Risk: The Role of Diet and Ageing","authors":"Mi Du, Ke Deng, Jiawei Yin, Chenkai Wu, Shixian Hu, Linxu Guo, Zhixin Luo, Maurizio Tonetti, Geerten‐Has E. Tjakkes, Anita Visser, Shaohua Ge, An Li","doi":"10.1111/jcpe.14122","DOIUrl":"https://doi.org/10.1111/jcpe.14122","url":null,"abstract":"AimMasticatory dysfunction due to tooth loss is a potentially modifiable risk for mortality, but the pathway behind that remains to be investigated. This prospective study aimed to examine the role of diet and ageing in the associations between chewing capacity and long‐term mortality.MethodsData were obtained from participants (aged ≥ 20) in the National Health Nutritional and Health Survey (NHANES 1999–2010, <jats:italic>n</jats:italic> = 22,900). The mortality follow‐up ended on 31 December 2019. Chewing capacity was determined by the number of functional tooth units (FTUs). Diet information in NHANES was collected using a 24‐h‐recall questionnaire, and diet quality was measured by three index‐based dietary patterns, namely the nutrition index (NI), energy‐adjusted dietary inflammatory index (E‐DII) and healthy eating index‐2015 (HEI‐2015). The biological ageing process was reflected using phenotypic age acceleration (PhenoAgeAccel) and frailty index. Mediation analyses were conducted to assess the role of diet quality in the association between FTUs and ageing, as well as the role of ageing in the association between impaired chewing capacity (ICC) and mortality.ResultsParticipants with more FTUs were found to have a slower biological ageing process. Diet quality scores were estimated to mediate 9.0%–23.0% of the association between chewing capacity and biological ageing. Multivariable Cox proportional hazards regression models found a positive association between ICC and all‐cause mortality (hazard ratio = 1.282 [95% confidence interval: 1.189–1.382]). Also, ICC was significantly associated with a 28.9% higher risk of mortality due to cardiovascular disease (CVD) and a 32.7% higher risk of mortality due to cancer. Mediation analyses indicated that PhenoAgeAccel mediated the effect of ICC on all‐cause, CVD and cancer mortality with proportions of 18.1%, 17.3% and 12.5%, respectively. Similar mediating proportions were observed in the frailty index (range: 11.6%–23.5%).ConclusionsICC was associated with poorer diet quality and accelerated ageing, resulting in higher mortality risk. Therefore, it is plausible that dietary interventions and oral rehabilitation would promote healthy longevity, although further investigations are needed.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"27 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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