Jarno Hakkers,Yvonne C M de Waal,Barzi Gareb,Henny J A Meijer,Gerry M Raghoebar
AIMTo analyse the effect of systemic amoxicillin and metronidazole on surgical peri-implantitis treatment with a follow-up period of 1 year.MATERIALS AND METHODSFifty-nine patients were randomly assigned to receive peri-implantitis surgery supplemented with (29 patients; test) or without (30 patients; control) systemic amoxicillin and metronidazole, in cases with no, one-wall or two-wall bony defects. Primary outcomes were mean peri-implant probing pocket depth (PPD) and mean peri-implant bleeding on probing (BOP); secondary outcomes included disease resolution (composite treatment outcome: residual probing depths ≤ 5 mm; no BOP in one or more probing site; no suppuration on probing), suppuration on probing (SOP) and radiographic marginal bone levels (MBLs), evaluated 3, 6, 9 and 12 months postoperatively (T3, T6, T9, T12). Linear and logistic mixed-effects models were employed.RESULTSBetween-group analyses showed that BOP was significantly lower in the test group compared to the control group at T9 (β = -10.57%, 95% CI: -20.17 to -0.97, p = 0.03) and T12 (β = -14.47%, 95% CI: -25.9 to -3.04, p = 0.01). No other parameters in the mixed-effects models showed statistically significant differences between groups at any timepoint.CONCLUSIONAccess-flap peri-implantitis surgery supplemented with systemic amoxicillin and metronidazole led to a statistically significant reduction in peri-implant BOP after 1 year, whereas no other parameters showed statistically significant differences between groups at any timepoint. The clinical implications of these differences should be interpreted with caution, as the isolated short-term effect does not translate into broader or sustained clinical benefit and must be weighed against the risks associated with systemic antibiotic use.
{"title":"Adjunctive Systemic Amoxicillin and Metronidazole Following Surgical Peri-Implantitis Treatment: A Single-Blind Randomised Controlled Trial With a 1-Year Follow-Up.","authors":"Jarno Hakkers,Yvonne C M de Waal,Barzi Gareb,Henny J A Meijer,Gerry M Raghoebar","doi":"10.1111/jcpe.70100","DOIUrl":"https://doi.org/10.1111/jcpe.70100","url":null,"abstract":"AIMTo analyse the effect of systemic amoxicillin and metronidazole on surgical peri-implantitis treatment with a follow-up period of 1 year.MATERIALS AND METHODSFifty-nine patients were randomly assigned to receive peri-implantitis surgery supplemented with (29 patients; test) or without (30 patients; control) systemic amoxicillin and metronidazole, in cases with no, one-wall or two-wall bony defects. Primary outcomes were mean peri-implant probing pocket depth (PPD) and mean peri-implant bleeding on probing (BOP); secondary outcomes included disease resolution (composite treatment outcome: residual probing depths ≤ 5 mm; no BOP in one or more probing site; no suppuration on probing), suppuration on probing (SOP) and radiographic marginal bone levels (MBLs), evaluated 3, 6, 9 and 12 months postoperatively (T3, T6, T9, T12). Linear and logistic mixed-effects models were employed.RESULTSBetween-group analyses showed that BOP was significantly lower in the test group compared to the control group at T9 (β = -10.57%, 95% CI: -20.17 to -0.97, p = 0.03) and T12 (β = -14.47%, 95% CI: -25.9 to -3.04, p = 0.01). No other parameters in the mixed-effects models showed statistically significant differences between groups at any timepoint.CONCLUSIONAccess-flap peri-implantitis surgery supplemented with systemic amoxicillin and metronidazole led to a statistically significant reduction in peri-implant BOP after 1 year, whereas no other parameters showed statistically significant differences between groups at any timepoint. The clinical implications of these differences should be interpreted with caution, as the isolated short-term effect does not translate into broader or sustained clinical benefit and must be weighed against the risks associated with systemic antibiotic use.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"3 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145961602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comment on Huoshen et al. (2025) ‘Pharmacovigilance-Based Identification and Mechanistic Exploration of Periodontitis-Related Drugs’","authors":"Jianxing Zhou, Weipeng Lai, Jiaping Zheng","doi":"10.1111/jcpe.70074","DOIUrl":"10.1111/jcpe.70074","url":null,"abstract":"","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 3","pages":"492-493"},"PeriodicalIF":6.8,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145937884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shrouk N. Elboray, Ola M. Ezzatt, Ahmad Salah, Asmaa Mohamed, Mahetab M. Abdalwahab
Aim To evaluate the diagnostic accuracy of thermographic imaging for differentiating healthy gingiva, site‐level inflammation and sites with clinical attachment loss. Subjects and Methods A calibrated periodontist examined 511 teeth in systemically healthy individuals attending the periodontology clinic. Clinical assessments were performed at buccal/facial sites within the anterior and premolar regions, with parameters including bleeding on probing (BOP), plaque index (PI), gingival index (GI), probing depth (PD) and clinical attachment level (CAL). Each tooth was classified into one of three subgroups based on CAL and BOP: clinically healthy sites (Group1/CH); inflamed sites without attachment loss (Group2/INF); and sites with clinical attachment loss (Group3/AL). Thermal imaging of the same sites was performed by a blinded operator. Diagnostic performance was assessed using receiver operating characteristic (ROC) curves as well as sensitivity and specificity analyses. Results The mean temperature values were 37.8°C ± 1.6°C for Group2/INF, 38.7°C ± 1.2°C for Group3/AL and 34.8°C ± 2.4°C for Group1/CH ( p < 0.001). ROC analysis resulted in an area under the curve (AUC) of 0.94 for Group2/INF and 0.86 for Group3/AL, high sensitivity (93% and 90.7%) and moderate to high specificity (83.8% and 64.5%), respectively, with significant correlations between thermal values, PI and CAL ( p < 0.001). Conclusions Thermographic imaging could effectively differentiate between clinically healthy sites and those showing site‐specific inflammation or attachment loss. These findings reflect periodontal status at the site level and are not intended for full‐mouth diagnosis of gingivitis or periodontitis. Trial Registration: The trial was registered at https://clinicaltrials.gov/ under the number (NCT06290414) on 14/8/2024
{"title":"Evaluation of the Accuracy of Infrared Thermographic Imaging for the Diagnosis of Periodontal Diseases: A Cross‐Sectional Study","authors":"Shrouk N. Elboray, Ola M. Ezzatt, Ahmad Salah, Asmaa Mohamed, Mahetab M. Abdalwahab","doi":"10.1111/jcpe.70066","DOIUrl":"https://doi.org/10.1111/jcpe.70066","url":null,"abstract":"Aim To evaluate the diagnostic accuracy of thermographic imaging for differentiating healthy gingiva, site‐level inflammation and sites with clinical attachment loss. Subjects and Methods A calibrated periodontist examined 511 teeth in systemically healthy individuals attending the periodontology clinic. Clinical assessments were performed at buccal/facial sites within the anterior and premolar regions, with parameters including bleeding on probing (BOP), plaque index (PI), gingival index (GI), probing depth (PD) and clinical attachment level (CAL). Each tooth was classified into one of three subgroups based on CAL and BOP: clinically healthy sites (Group1/CH); inflamed sites without attachment loss (Group2/INF); and sites with clinical attachment loss (Group3/AL). Thermal imaging of the same sites was performed by a blinded operator. Diagnostic performance was assessed using receiver operating characteristic (ROC) curves as well as sensitivity and specificity analyses. Results The mean temperature values were 37.8°C ± 1.6°C for Group2/INF, 38.7°C ± 1.2°C for Group3/AL and 34.8°C ± 2.4°C for Group1/CH ( <jats:italic>p</jats:italic> < 0.001). ROC analysis resulted in an area under the curve (AUC) of 0.94 for Group2/INF and 0.86 for Group3/AL, high sensitivity (93% and 90.7%) and moderate to high specificity (83.8% and 64.5%), respectively, with significant correlations between thermal values, PI and CAL ( <jats:italic>p</jats:italic> < 0.001). Conclusions Thermographic imaging could effectively differentiate between clinically healthy sites and those showing site‐specific inflammation or attachment loss. These findings reflect periodontal status at the site level and are not intended for full‐mouth diagnosis of gingivitis or periodontitis. Trial Registration: The trial was registered at <jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"https://clinicaltrials.gov/\">https://clinicaltrials.gov/</jats:ext-link> under the number (NCT06290414) on 14/8/2024","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"1 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145847294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Charlene E. Goh, Bruno Bohn, Jeanine M. Genkinger, Rebecca Molinsky, Sumith Roy, Bruce J. Paster, Ching‐Yuan Chen, Stephen Johnson, Melana Yuzefpolskaya, Paolo C. Colombo, Michael Rosenbaum, Rob Knight, Moïse Desvarieux, Panos N. Papapanou, David R. Jacobs, Ryan T. Demmer
Aims To investigate whether the association between the nitrite‐generating capacity of the subgingival microbiome and early cardiometabolic risk biomarkers varies by dietary nitrate intake. Materials and Methods Cross‐sectional data from 668 participants (mean age 31 ± 9 years, 73% women) were analysed. Dietary nitrate intake was calculated from food frequency questionnaires. Subgingival 16S rRNA sequencing (Illumina, MiSeq) and PICRUSt2 estimated microbial genes. The Microbiome‐Induced Nitric Oxide Enrichment Score (MINES) was calculated as a ratio of microbial gene abundances representing enhanced net capacity for NO generation. Adjusted multivariable linear models regressed cardiometabolic risk biomarkers (HbA1c, glucose, insulin, insulin resistance (HOMA‐IR), blood pressure) on nitrate intake and MINES together with a MINES × nitrate intake interaction term. Results Mean nitrate intake was 190 ± 171 mg/day. Significant interactions of MINES and nitrate intake were observed for insulin and HOMA‐IR ( p < 0.05). Among participants with a low MINES, higher nitrate intake was associated with lower HOMA‐IR (1.2 [1.1–1.4] vs. 1.5 [1.3–1.6]; p = 0.002), but levels were similar in those with high MINES ( p = 0.84). Conclusions A biomarker of higher microbial NO‐generating capacity in subgingival plaque is associated with lower insulin and insulin resistance among individuals with lower dietary nitrate intake. Future trials evaluating the cardiometabolic benefits of nitrate‐rich diets should incorporate measures of the entire oral microbiome.
{"title":"Dietary Nitrate Intake and 16S rRNA ‐Inferred Nitrite‐Generating Capacity of the Subgingival Microbiome May Influence Glucose Metabolism: Results From the Oral Infections Glucose Intolerance and Insulin Resistance Study ( ORIGINS )","authors":"Charlene E. Goh, Bruno Bohn, Jeanine M. Genkinger, Rebecca Molinsky, Sumith Roy, Bruce J. Paster, Ching‐Yuan Chen, Stephen Johnson, Melana Yuzefpolskaya, Paolo C. Colombo, Michael Rosenbaum, Rob Knight, Moïse Desvarieux, Panos N. Papapanou, David R. Jacobs, Ryan T. Demmer","doi":"10.1111/jcpe.70084","DOIUrl":"https://doi.org/10.1111/jcpe.70084","url":null,"abstract":"Aims To investigate whether the association between the nitrite‐generating capacity of the subgingival microbiome and early cardiometabolic risk biomarkers varies by dietary nitrate intake. Materials and Methods Cross‐sectional data from 668 participants (mean age 31 ± 9 years, 73% women) were analysed. Dietary nitrate intake was calculated from food frequency questionnaires. Subgingival 16S rRNA sequencing (Illumina, MiSeq) and PICRUSt2 estimated microbial genes. The Microbiome‐Induced Nitric Oxide Enrichment Score (MINES) was calculated as a ratio of microbial gene abundances representing enhanced net capacity for NO generation. Adjusted multivariable linear models regressed cardiometabolic risk biomarkers (HbA1c, glucose, insulin, insulin resistance (HOMA‐IR), blood pressure) on nitrate intake and MINES together with a MINES × nitrate intake interaction term. Results Mean nitrate intake was 190 ± 171 mg/day. Significant interactions of MINES and nitrate intake were observed for insulin and HOMA‐IR ( <jats:italic>p</jats:italic> < 0.05). Among participants with a low MINES, higher nitrate intake was associated with lower HOMA‐IR (1.2 [1.1–1.4] vs. 1.5 [1.3–1.6]; <jats:italic>p</jats:italic> = 0.002), but levels were similar in those with high MINES ( <jats:italic>p</jats:italic> = 0.84). Conclusions A biomarker of higher microbial NO‐generating capacity in subgingival plaque is associated with lower insulin and insulin resistance among individuals with lower dietary nitrate intake. Future trials evaluating the cardiometabolic benefits of nitrate‐rich diets should incorporate measures of the entire oral microbiome.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"22 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145830262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Patrick Nafz, Thomas Kocher, Christiane Pink, Sebastian‐Edgar Baumeister, Stefan Reckelkamm, Stefanie Samietz, Sonya Nafz, Henry Völzke, Philipp Kanzow, Birte Holtfreter
Aim To investigate the association between dental restorations and adjacent periodontal status over a 7‐year period, using data from a population‐based cohort study. Materials and Methods We used 7‐year follow‐up data on the restorative and periodontal statuses of 88,793 tooth surfaces from 2158 SHIP‐TREND (Study of Health in Pomerania) participants. Using confounder‐adjusted and inverse‐probability‐weighted generalised estimating equations, we estimated the associations of restoration status with bleeding on probing (BOP), probing depth (PD) and clinical attachment level (CAL). Results Surfaces with dental restorations had significantly poorer periodontal outcomes than sound surfaces, with crowns having the greatest impact. At follow‐up, filled and crowned surfaces presented higher proportions of adjacent sites with BOP (18.5% and 22.4%, respectively) compared to sound surfaces (15.8%). Similarly, adjusted average PD was 1.93 mm adjacent to sound surfaces, 1.99 mm adjacent to surfaces with fillings and 2.14 mm adjacent to surfaces with crowns. The results remained consistent when the effects of incidentally placed fillings and crowns on follow‐up periodontal status were evaluated. Although effect modification by surface type was observed, no consistent patterns emerged across the different outcomes. Conclusion Dental restorations can have an adverse effect on periodontal health, emphasising the critical need for precise restorative techniques and post‐treatment maintenance.
{"title":"Associations Between Restoration Margins and Adjacent Periodontal Status—Longitudinal Results From SHIP‐TREND","authors":"Patrick Nafz, Thomas Kocher, Christiane Pink, Sebastian‐Edgar Baumeister, Stefan Reckelkamm, Stefanie Samietz, Sonya Nafz, Henry Völzke, Philipp Kanzow, Birte Holtfreter","doi":"10.1111/jcpe.70082","DOIUrl":"https://doi.org/10.1111/jcpe.70082","url":null,"abstract":"Aim To investigate the association between dental restorations and adjacent periodontal status over a 7‐year period, using data from a population‐based cohort study. Materials and Methods We used 7‐year follow‐up data on the restorative and periodontal statuses of 88,793 tooth surfaces from 2158 SHIP‐TREND (Study of Health in Pomerania) participants. Using confounder‐adjusted and inverse‐probability‐weighted generalised estimating equations, we estimated the associations of restoration status with bleeding on probing (BOP), probing depth (PD) and clinical attachment level (CAL). Results Surfaces with dental restorations had significantly poorer periodontal outcomes than sound surfaces, with crowns having the greatest impact. At follow‐up, filled and crowned surfaces presented higher proportions of adjacent sites with BOP (18.5% and 22.4%, respectively) compared to sound surfaces (15.8%). Similarly, adjusted average PD was 1.93 mm adjacent to sound surfaces, 1.99 mm adjacent to surfaces with fillings and 2.14 mm adjacent to surfaces with crowns. The results remained consistent when the effects of incidentally placed fillings and crowns on follow‐up periodontal status were evaluated. Although effect modification by surface type was observed, no consistent patterns emerged across the different outcomes. Conclusion Dental restorations can have an adverse effect on periodontal health, emphasising the critical need for precise restorative techniques and post‐treatment maintenance.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"23 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145807510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Emilio Couso‐Queiruga, Manrique Fonseca, Vivianne Chappuis, Gustavo‐Avila Ortiz, Giovanni E. Salvi, Frank Schwarz, Clemens Raabe
Objectives To compare the long‐term survival rate and prevalence of peri‐implant diseases between bone‐level (BL) and tissue‐level (TL) titanium implants. The secondary objective was to assess the effect of implant diameter and other risk indicators of peri‐implant diseases on the outcomes of implant therapy. Materials and Methods Adult patients with at least one non‐molar implant‐supported prosthesis (ISP) were included in the study. Relevant clinical and radiographic outcomes, along with patient‐related, anatomical, surgical and prosthetic‐related factors, were analysed. Results A total of 266 patients and 336 ISPs were included after a mean follow‐up of 11.2 ± 1.5 years. Implant survival rates at the implant level were 99.4% and 98.2% for BL and TL implants, respectively. The prevalence of peri‐implant health, mucositis and peri‐implantitis was comparable between BL (21.1%, 67.5% and 11.4%, respectively) and TL implants (20.5%, 70.5% and 9.0%). Implants with a diameter of 3.3 mm showed lower peri‐implantitis rates (7.2%) compared to those with 4.1 mm (13.3%; p = 0.02). Notably, 3.3 mm TL implants exhibited a significantly lower peri‐implantitis rate (4.8%) than BL implants (9.6%; p < 0.001). Multilevel regression at the implant level showed that parafunctional habits (OR = 0.33, 95% CI: 0.12–0.91) and greater mucosal thickness (OR = 0.44, 95% CI: 0.32–0.60) were cross‐sectionally associated with decreased odds of mucositis, whereas higher plaque scores were cross‐sectionally associated with increased odds (OR = 1.29, 95% CI: 1.03–1.61). Age was cross‐sectionally associated with peri‐implantitis (OR = 0.96, 95% CI: 0.93–0.99), higher plaque score (OR = 1.45, 95% CI: 1.11–1.90), larger implant diameter (OR = 2.98, 95% CI: 1.19–7.45) and smoking (OR = 4.54, 95% CI: 1.42–14.5), while greater mucosal thickness (OR = 0.17, 95% CI: 0.08–0.37) was cross‐sectionally associated with a reduced risk of developing this condition. Conclusions BL and TL implants at non‐molar sites exhibited comparable survival and peri‐implant disease rates. However, TL implants with 3.3 mm diameter showed lower peri‐implantitis rates. A higher plaque score increased the risk of both mucositis and peri‐implantitis, whereas smoking was a strong risk indicator for peri‐implantitis. Greater mucosal thickness was protective against both conditions.
{"title":"Bone‐Level Versus Tissue‐Level Titanium Dental Implants: A Comparative Cross‐Sectional Study","authors":"Emilio Couso‐Queiruga, Manrique Fonseca, Vivianne Chappuis, Gustavo‐Avila Ortiz, Giovanni E. Salvi, Frank Schwarz, Clemens Raabe","doi":"10.1111/jcpe.70080","DOIUrl":"https://doi.org/10.1111/jcpe.70080","url":null,"abstract":"Objectives To compare the long‐term survival rate and prevalence of peri‐implant diseases between bone‐level (BL) and tissue‐level (TL) titanium implants. The secondary objective was to assess the effect of implant diameter and other risk indicators of peri‐implant diseases on the outcomes of implant therapy. Materials and Methods Adult patients with at least one non‐molar implant‐supported prosthesis (ISP) were included in the study. Relevant clinical and radiographic outcomes, along with patient‐related, anatomical, surgical and prosthetic‐related factors, were analysed. Results A total of 266 patients and 336 ISPs were included after a mean follow‐up of 11.2 ± 1.5 years. Implant survival rates at the implant level were 99.4% and 98.2% for BL and TL implants, respectively. The prevalence of peri‐implant health, mucositis and peri‐implantitis was comparable between BL (21.1%, 67.5% and 11.4%, respectively) and TL implants (20.5%, 70.5% and 9.0%). Implants with a diameter of 3.3 mm showed lower peri‐implantitis rates (7.2%) compared to those with 4.1 mm (13.3%; <jats:italic>p</jats:italic> = 0.02). Notably, 3.3 mm TL implants exhibited a significantly lower peri‐implantitis rate (4.8%) than BL implants (9.6%; <jats:italic>p</jats:italic> < 0.001). Multilevel regression at the implant level showed that parafunctional habits (OR = 0.33, 95% CI: 0.12–0.91) and greater mucosal thickness (OR = 0.44, 95% CI: 0.32–0.60) were cross‐sectionally associated with decreased odds of mucositis, whereas higher plaque scores were cross‐sectionally associated with increased odds (OR = 1.29, 95% CI: 1.03–1.61). Age was cross‐sectionally associated with peri‐implantitis (OR = 0.96, 95% CI: 0.93–0.99), higher plaque score (OR = 1.45, 95% CI: 1.11–1.90), larger implant diameter (OR = 2.98, 95% CI: 1.19–7.45) and smoking (OR = 4.54, 95% CI: 1.42–14.5), while greater mucosal thickness (OR = 0.17, 95% CI: 0.08–0.37) was cross‐sectionally associated with a reduced risk of developing this condition. Conclusions BL and TL implants at non‐molar sites exhibited comparable survival and peri‐implant disease rates. However, TL implants with 3.3 mm diameter showed lower peri‐implantitis rates. A higher plaque score increased the risk of both mucositis and peri‐implantitis, whereas smoking was a strong risk indicator for peri‐implantitis. Greater mucosal thickness was protective against both conditions.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"3 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145807679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Spyridon K. Kouris, Yiorgos A. Bobetsis, Sophia Lionaki, Panagiotis N. Kanellopoulos, George Maropoulos, Panagiotis A. Koromantzos, Athanasios D. Protogerou, Phoebus N. Madianos
Aim
To investigate the effect of non-surgical periodontal therapy (NSPT) on renal function and glycaemic control in chronic kidney disease (CKD) patients over a 6-month observation period, through a randomised clinical trial (RCT).
Materials and Methods
A total of 53 patients participated: 27 in the intervention group (IG) and 26 in the control group (CG). Outcomes included estimated glomerular filtration rate (eGFR, calculated using serum creatinine or both serum creatinine and cystatin-C), urine albumin to urine creatinine ratio (uACR) and serum high-sensitivity C-reactive protein (hsCRP) levels. Glycaemic control was assessed using HbA1c levels in patients with diabetes mellitus (DM).
Results
Intra-group ΔeGFR levels were 10.26 mL/min/1.73 m2 (p < 0.001) and 11.17 mL/min/1.73 m2 (p < 0.001), depending on the equation used, favouring IG. hsCRP levels were reduced by 84% (p < 0.001) compared to CG; ΔHbA1c decreased by 0.54% (p = 0.003) at 6 months. uACR was lower but only marginally (not statistically significant) in IG.
Conclusion
Despite its limitations, this RCT shows that NSPT enhances renal function—increasing eGFR and decreasing hsCRP—and improves glycaemic control in CKD patients with DM.
{"title":"The Effect of Periodontal Therapy on Renal Function and Diabetes Control in Patients With Chronic Kidney Disease. A Randomised Controlled Clinical Trial","authors":"Spyridon K. Kouris, Yiorgos A. Bobetsis, Sophia Lionaki, Panagiotis N. Kanellopoulos, George Maropoulos, Panagiotis A. Koromantzos, Athanasios D. Protogerou, Phoebus N. Madianos","doi":"10.1111/jcpe.70059","DOIUrl":"10.1111/jcpe.70059","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To investigate the effect of non-surgical periodontal therapy (NSPT) on renal function and glycaemic control in chronic kidney disease (CKD) patients over a 6-month observation period, through a randomised clinical trial (RCT).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A total of 53 patients participated: 27 in the intervention group (IG) and 26 in the control group (CG). Outcomes included estimated glomerular filtration rate (eGFR, calculated using serum creatinine or both serum creatinine and cystatin-C), urine albumin to urine creatinine ratio (uACR) and serum high-sensitivity C-reactive protein (hsCRP) levels. Glycaemic control was assessed using HbA1c levels in patients with diabetes mellitus (DM).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Intra-group ΔeGFR levels were 10.26 mL/min/1.73 m<sup>2</sup> (<i>p</i> < 0.001) and 11.17 mL/min/1.73 m<sup>2</sup> (<i>p</i> < 0.001), depending on the equation used, favouring IG. hsCRP levels were reduced by 84% (<i>p</i> < 0.001) compared to CG; ΔHbA1c decreased by 0.54% (<i>p</i> = 0.003) at 6 months. uACR was lower but only marginally (not statistically significant) in IG.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Despite its limitations, this RCT shows that NSPT enhances renal function—increasing eGFR and decreasing hsCRP—and improves glycaemic control in CKD patients with DM.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 3","pages":"362-372"},"PeriodicalIF":6.8,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145807509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim To identify novel non‐syndromic hereditary gingival fibromatosis (nsHGF)–associated pathogenic variants and discover therapeutic targets for innovative, minimally invasive therapies. Materials and Methods Whole‐genome sequencing was performed to identify the pathogenic variant in a family with nsHGF. Levels of fibrosis markers and the yes‐associated protein/transcriptional coactivator with PDZ‐binding motif (YAP/TAZ) in gingival fibroblasts were measured by qPCR, western blot and immunofluorescence. Conditioned medium from gingival fibroblasts was used to treat THP‐1‐derived macrophages. In vivo pro‐fibrotic behaviour of CHYMASE ‐silenced gingival fibroblasts and verteporfin‐loaded exosome efficacy were evaluated in NOD/SCID mice. Results We identified a novel CHYMASE ( CMA1 ) nonsense mutation (c.114C>A, p.Tyr38*) in the nsHGF family. This mutation caused chymase deficiency in the patient's gingival fibroblasts, directly leading to extracellular matrix (ECM) overproduction through YAP/TAZ activation. Moreover, CHYMASE ‐silenced gingival fibroblasts promoted interleukin‐6 (IL‐6) secretion by macrophages, thereby amplifying pro‐fibrotic responses in gingival fibroblasts. The YAP inhibitor verteporfin suppressed ECM overproduction in CHYMASE ‐silenced gingival fibroblasts. In vivo, topical administration of verteporfin‐loaded exosomes significantly attenuated chymase deficiency–induced fibrosis. Conclusion Our findings support the pathogenic role of the CHYMASE mutation in nsHGF, establish chymase deficiency and consequent YAP/TAZ activation as the underlying mechanism and propose verteporfin‐loaded exosomes as a promising therapeutic strategy for nsHGF‐associated gingival overgrowth.
目的发现新的非综合征性遗传性牙龈纤维瘤病（nsHGF）相关的致病变异，并发现创新的微创治疗靶点。材料和方法采用全基因组测序方法鉴定一个nsHGF家族的致病变异。通过qPCR、western blot和免疫荧光检测牙龈成纤维细胞中纤维化标志物和yes相关蛋白/带PDZ结合基序的转录共激活因子（YAP/TAZ）的水平。使用牙龈成纤维细胞的条件培养基处理THP‐1来源的巨噬细胞。在NOD/SCID小鼠中评估了CHYMASE沉默的牙龈成纤维细胞的体内促纤维化行为和载维替porfin的外泌体功效。结果在nsHGF家族中发现了一个新的CHYMASE （CMA1）无义突变（c.114C> a, p.Tyr38*）。这种突变导致患者的牙龈成纤维细胞中乳糜酶缺乏，通过YAP/TAZ激活直接导致细胞外基质（ECM）过量产生。此外，CHYMASE沉默的牙龈成纤维细胞促进巨噬细胞分泌白细胞介素- 6 (IL - 6)，从而增强了牙龈成纤维细胞的促纤维化反应。YAP抑制剂维替波芬抑制CHYMASE沉默的牙龈成纤维细胞中ECM的过量产生。在体内，局部施用载维替波芬的外泌体可显著减轻乳糜酶缺乏症引起的纤维化。结论我们的研究结果支持了CHYMASE突变在nsHGF中的致病作用，确定了CHYMASE缺陷和随后的YAP/TAZ激活是潜在的机制，并提出了满载维替波芬的外泌体作为治疗nsHGF相关牙龈过度生长的有希望的治疗策略。
{"title":"Non‐Syndromic Hereditary Gingival Fibromatosis Driven by Chymase Deficiency Is Attenuated by Verteporfin‐Loaded Exosomes","authors":"Xin Chen, Yuqing Guo, Yangqiao Qing, Runze Li, Haotian Luo, Hio Cheng Ieong, Bingyan Guo, Zichun Huang, Yungshan Teng, Ruoyu Li, Wenfeng Li, Danying Chen, Yang Cao, Weicai Wang, Chen Zhou","doi":"10.1111/jcpe.70077","DOIUrl":"https://doi.org/10.1111/jcpe.70077","url":null,"abstract":"Aim To identify novel non‐syndromic hereditary gingival fibromatosis (nsHGF)–associated pathogenic variants and discover therapeutic targets for innovative, minimally invasive therapies. Materials and Methods Whole‐genome sequencing was performed to identify the pathogenic variant in a family with nsHGF. Levels of fibrosis markers and the yes‐associated protein/transcriptional coactivator with PDZ‐binding motif (YAP/TAZ) in gingival fibroblasts were measured by qPCR, western blot and immunofluorescence. Conditioned medium from gingival fibroblasts was used to treat THP‐1‐derived macrophages. In vivo pro‐fibrotic behaviour of <jats:italic>CHYMASE</jats:italic> ‐silenced gingival fibroblasts and verteporfin‐loaded exosome efficacy were evaluated in NOD/SCID mice. Results We identified a novel <jats:italic>CHYMASE</jats:italic> ( <jats:italic>CMA1</jats:italic> ) nonsense mutation (c.114C>A, p.Tyr38*) in the nsHGF family. This mutation caused chymase deficiency in the patient's gingival fibroblasts, directly leading to extracellular matrix (ECM) overproduction through YAP/TAZ activation. Moreover, <jats:italic>CHYMASE</jats:italic> ‐silenced gingival fibroblasts promoted interleukin‐6 (IL‐6) secretion by macrophages, thereby amplifying pro‐fibrotic responses in gingival fibroblasts. The YAP inhibitor verteporfin suppressed ECM overproduction in <jats:italic>CHYMASE</jats:italic> ‐silenced gingival fibroblasts. In vivo, topical administration of verteporfin‐loaded exosomes significantly attenuated chymase deficiency–induced fibrosis. Conclusion Our findings support the pathogenic role of the <jats:italic>CHYMASE</jats:italic> mutation in nsHGF, establish chymase deficiency and consequent YAP/TAZ activation as the underlying mechanism and propose verteporfin‐loaded exosomes as a promising therapeutic strategy for nsHGF‐associated gingival overgrowth.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"39 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145730642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thomas Kocher, Sebastian-Edgar Baumeister, Henry Völzke, Matthias Nauck, Peter Meisel, Karsten Suhre, Uwe Völker, Nele Friedrich, Birte Holtfreter
Background
Theobromine, a methylxanthine mainly found in chocolate, has been suggested to possess various health-promoting properties. This study aimed to investigate the long-term effect of salivary theobromine levels on periodontitis severity using 7- and 10-year follow-up data from the prospective Studies of Health in Pomerania (SHIP-TREND and SHIP-START).
Materials and Methods
We conducted a non-targeted metabolomics analysis of salivary methylxanthines in 679 participants from SHIP-TREND and 953 participants from SHIP-START. Inverse-probability-of-treatment-weighted generalised linear models were used to assess the relationship between salivary theobromine and periodontal variables, including bleeding on probing, probing depth and clinical attachment loss.
Results
Higher salivary theobromine levels were significantly associated with improved periodontal health, as evidenced by lower mean probing depth and a reduced percentage of sites with probing depth ≥ 3 mm. The results were successfully replicated in the SHIP-START data and extended to a lower clinical attachment loss.
Discussion
Our cohort studies suggest that elevated salivary theobromine levels are associated with improved periodontal parameters over 7 and 10 years. These results indicate the potential for theobromine-containing products to support periodontal health, warranting further investigation through randomised controlled trials.
{"title":"Elevated Salivary Theobromine and Long-Term Improvement of Periodontal Health in Two Cohort Studies","authors":"Thomas Kocher, Sebastian-Edgar Baumeister, Henry Völzke, Matthias Nauck, Peter Meisel, Karsten Suhre, Uwe Völker, Nele Friedrich, Birte Holtfreter","doi":"10.1111/jcpe.70072","DOIUrl":"10.1111/jcpe.70072","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Theobromine, a methylxanthine mainly found in chocolate, has been suggested to possess various health-promoting properties. This study aimed to investigate the long-term effect of salivary theobromine levels on periodontitis severity using 7- and 10-year follow-up data from the prospective Studies of Health in Pomerania (SHIP-TREND and SHIP-START).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>We conducted a non-targeted metabolomics analysis of salivary methylxanthines in 679 participants from SHIP-TREND and 953 participants from SHIP-START. Inverse-probability-of-treatment-weighted generalised linear models were used to assess the relationship between salivary theobromine and periodontal variables, including bleeding on probing, probing depth and clinical attachment loss.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Higher salivary theobromine levels were significantly associated with improved periodontal health, as evidenced by lower mean probing depth and a reduced percentage of sites with probing depth ≥ 3 mm. The results were successfully replicated in the SHIP-START data and extended to a lower clinical attachment loss.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Discussion</h3>\u0000 \u0000 <p>Our cohort studies suggest that elevated salivary theobromine levels are associated with improved periodontal parameters over 7 and 10 years. These results indicate the potential for theobromine-containing products to support periodontal health, warranting further investigation through randomised controlled trials.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 3","pages":"445-454"},"PeriodicalIF":6.8,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145730639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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