Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38429
Shouyu Zhang, Yuanxin Bi, Zuli Wu
{"title":"Spatial distribution pattern of Sargassum horneri around Gouqi Island,Shengsi,China","authors":"Shouyu Zhang, Yuanxin Bi, Zuli Wu","doi":"10.3724/SP.J.1231.2013.38429","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38429","url":null,"abstract":"","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69966376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38430
X. Yi, Wenbing Zhang, K. Mai, J. Shentu
An 8-week feeding trial was conducted to investigate the effects of dietary fish oil replaced with rapeseed oil on growth,muscle fatty acid compositions and skin color of large yellow croaker(initial weight 13.56±0.05 g).Five isoproteic and isolipidic experimental diets were formulated with 0%,25%,50%,75% and 100% replacement of fish oil by rapeseed oil.Results showed that these replacements did not significantly influence the survival rate(SR)and specific growth rate(SGR)(P0.05).However,the feed conversion ratio(FCR)was increased with the increasing of replacement levels.Furthermore,FCR in the group of 100% rapeseed oil was significantly higher than that of 100% dietary fish oil(P0.05).No significant difference was observed in the whole body compositions(P0.05).The concentrations of C18∶ 0,C18∶ 1,C18∶ 2n-6,C18∶ 3n-3 and the saturated fatty acid(SFA),except C20∶ 4n-6 and C22∶ 5n-3 in muscle,were elevated with the increasing of dietary rapeseed oil levels.There were no significant differences in yellowness(b*)of dorsal and ventral skin among the five treatments(P0.05).Lightness(L*)of the dorsal skin in the treatment with 100% dietary rapeseed oil was significantly higher than that with 100% dietary fish oil(P0.05).However,L* of the ventral skin was not significantly different among the all treatments(P0.05).Higher redness(a*)of ventral skin and lower a* of dorsal skin were found in fish fed with 0% rapeseed oil.This study suggested that there were no significant effects of replacement of dietary fish oil by rapeseed oil on growth and body composition.However,the fatty acid composition of muscle and the skin color were significantly influenced.
{"title":"Effects of dietary fish oil replaced with rapeseed oil on the growth,fatty acid composition and skin color of large yellow croaker(Larimichthys crocea)","authors":"X. Yi, Wenbing Zhang, K. Mai, J. Shentu","doi":"10.3724/SP.J.1231.2013.38430","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38430","url":null,"abstract":"An 8-week feeding trial was conducted to investigate the effects of dietary fish oil replaced with rapeseed oil on growth,muscle fatty acid compositions and skin color of large yellow croaker(initial weight 13.56±0.05 g).Five isoproteic and isolipidic experimental diets were formulated with 0%,25%,50%,75% and 100% replacement of fish oil by rapeseed oil.Results showed that these replacements did not significantly influence the survival rate(SR)and specific growth rate(SGR)(P0.05).However,the feed conversion ratio(FCR)was increased with the increasing of replacement levels.Furthermore,FCR in the group of 100% rapeseed oil was significantly higher than that of 100% dietary fish oil(P0.05).No significant difference was observed in the whole body compositions(P0.05).The concentrations of C18∶ 0,C18∶ 1,C18∶ 2n-6,C18∶ 3n-3 and the saturated fatty acid(SFA),except C20∶ 4n-6 and C22∶ 5n-3 in muscle,were elevated with the increasing of dietary rapeseed oil levels.There were no significant differences in yellowness(b*)of dorsal and ventral skin among the five treatments(P0.05).Lightness(L*)of the dorsal skin in the treatment with 100% dietary rapeseed oil was significantly higher than that with 100% dietary fish oil(P0.05).However,L* of the ventral skin was not significantly different among the all treatments(P0.05).Higher redness(a*)of ventral skin and lower a* of dorsal skin were found in fish fed with 0% rapeseed oil.This study suggested that there were no significant effects of replacement of dietary fish oil by rapeseed oil on growth and body composition.However,the fatty acid composition of muscle and the skin color were significantly influenced.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69966448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38443
Jun Liang, Weiding Wang, Hanxiang Xu, Yong-dong Zhou, Kaida Xu
It is necessary to establish a highly efficient method of marking,which will provide technical guarantee for a more accurate assessment of stock-enhancement effect of the cuttlefish(Sepiella japonica).The cuttlefish were chosen as the experimental material in this study.The larval cuttlefish were immersed in alizarin complexone(ALC)to dye the cuttlebone.The experiment was divided into 5 groups with concentration gradient and 2 time periods,and doral mantle length,body weight and survival rate of cuttlefish were measured and observed.The trial group and control group(50 ind/group)were reared in 2.5 m diameter circular concrete ponds,respectively.The cuttlefish were sampled randomly in order to examine the dye-retention of the tagged cuttlebone after being tagged 5,12,19,26 d.Moreover,the tagged cuttlefish were examined at high temperature treatment for 5 min.The results showed that the optimised dye concentration and immerged time were 90 mg/L and 24 h,respectively.The doral mantle length of cuttlefish had a significant positive correlation with body weight(P0.01),and there was no significant difference in growth between trial group and control group(P0.05).The tagged oval pink ring was still clear in the cuttlebone even after being tagged 26 d.The range and color of tagging parts were not changed before and after high temperature treatment.Additionally,the proposed method has effect on both internal signs and external signs,and the survival rate and dye-retention rate of the tagged cuttlefish are 100%.It is concluded that this method can be successfully applied to marking cuttlefish,and offer a number of benefits,including simple,efficient,high sample throughput,and a wide range of application prospects.
{"title":"A fluorescent staining method for marking the cuttlefish(Sepiella japonica)","authors":"Jun Liang, Weiding Wang, Hanxiang Xu, Yong-dong Zhou, Kaida Xu","doi":"10.3724/SP.J.1231.2013.38443","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38443","url":null,"abstract":"It is necessary to establish a highly efficient method of marking,which will provide technical guarantee for a more accurate assessment of stock-enhancement effect of the cuttlefish(Sepiella japonica).The cuttlefish were chosen as the experimental material in this study.The larval cuttlefish were immersed in alizarin complexone(ALC)to dye the cuttlebone.The experiment was divided into 5 groups with concentration gradient and 2 time periods,and doral mantle length,body weight and survival rate of cuttlefish were measured and observed.The trial group and control group(50 ind/group)were reared in 2.5 m diameter circular concrete ponds,respectively.The cuttlefish were sampled randomly in order to examine the dye-retention of the tagged cuttlebone after being tagged 5,12,19,26 d.Moreover,the tagged cuttlefish were examined at high temperature treatment for 5 min.The results showed that the optimised dye concentration and immerged time were 90 mg/L and 24 h,respectively.The doral mantle length of cuttlefish had a significant positive correlation with body weight(P0.01),and there was no significant difference in growth between trial group and control group(P0.05).The tagged oval pink ring was still clear in the cuttlebone even after being tagged 26 d.The range and color of tagging parts were not changed before and after high temperature treatment.Additionally,the proposed method has effect on both internal signs and external signs,and the survival rate and dye-retention rate of the tagged cuttlefish are 100%.It is concluded that this method can be successfully applied to marking cuttlefish,and offer a number of benefits,including simple,efficient,high sample throughput,and a wide range of application prospects.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69967329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38521
Liming Xu, Hongbai Liu, Lu Tongyan
Matrix protein is one of the structure proteins of infectious haematopoietic necrosis virus(IHNV),and its synthesis leads to apoptosis or programmed cell death in the transfected cells.To study its structure and function,the open reading frame of matrix protein gene was amplified by RT-PCR from IHNV-Sn isolate and cloned into plasmid pET27b(+)vector.The structure and characteristics of the gene were studied by means of bioinformatics software.The results are described as follows:the length of the M gene,encoding 195 aa,was 588 bp.The molecular weight was 21.88 ku and the isoelectric point was 9.35.The matrix protein was rich in serine,threonine and alkaline amino acid,and was composed of plenty of α-helix,extended β and coil.The protein is hydrophobic with no signal peptide and transmembrane regions.The antigenicity of matrix protein was good.According to the protein structure prediction,there might be no N-glycosylation sites,and there were 7 O-glycosylation sites and 15 phosphorylation sites in matrix protein.Phylogenetic analysis showed that the IHNV-Sn isolate was in the same branch with the isolates from America.The study established the foundation for genetic background information,pathogenesis,molecular epidemiology research of IHNV.
{"title":"Cloning and bioinformatics analysis of matrix protein from IHNV-Sn isolate","authors":"Liming Xu, Hongbai Liu, Lu Tongyan","doi":"10.3724/SP.J.1231.2013.38521","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38521","url":null,"abstract":"Matrix protein is one of the structure proteins of infectious haematopoietic necrosis virus(IHNV),and its synthesis leads to apoptosis or programmed cell death in the transfected cells.To study its structure and function,the open reading frame of matrix protein gene was amplified by RT-PCR from IHNV-Sn isolate and cloned into plasmid pET27b(+)vector.The structure and characteristics of the gene were studied by means of bioinformatics software.The results are described as follows:the length of the M gene,encoding 195 aa,was 588 bp.The molecular weight was 21.88 ku and the isoelectric point was 9.35.The matrix protein was rich in serine,threonine and alkaline amino acid,and was composed of plenty of α-helix,extended β and coil.The protein is hydrophobic with no signal peptide and transmembrane regions.The antigenicity of matrix protein was good.According to the protein structure prediction,there might be no N-glycosylation sites,and there were 7 O-glycosylation sites and 15 phosphorylation sites in matrix protein.Phylogenetic analysis showed that the IHNV-Sn isolate was in the same branch with the isolates from America.The study established the foundation for genetic background information,pathogenesis,molecular epidemiology research of IHNV.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69967805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38523
Yujuan Wang, Xiuhua Wang, Wenbao Han, Rui Wang, Jie Huang
The correlation between hemolysis time and concentration of saponin(2.5,5,12.5,25,50 and 125 mg/L)was analyzed via microscope observation.In vitro hemolysis rate of turbot blood cells in different concentrations of saponin(2.5,5,12.5,25 and 50 mg/L)at 20 and 4 ℃,for different time duration were tested respectively.To investigate the toxicity of saponin extracted from Quillaja saponaria to turbot(Scophthalmus maximus),median lethal concentration(LC50)was calculated by immersing the turbot in sea water with different concentrations of saponin.In addition,the lactic dehydrogenase(LDH)activities in serum of turbot after bathing in saponin seawater solution(0,5,25 and 45 mg/L)were analyzed.The results indicated that the correlation between hemolysis time of erythrocytes and saponin concentration showed a logarithmic negative correlation(R2=0.98),and that saponin could also induce hemolysis to leukocytes of turbot.Saponin in concentration of 50 mg/L could result in 100% hemolysis at 20 ℃ for 5 min,in contrast,only 42.2% hemolysis was observed at 4 ℃.The 24 h LC50 of saponin for S.maximus was 64.85 mg/L.In this report,from cell level,the evaluation of the toxicity of saponin to blood cells for hemolysis was made,which will theoretically support its safe application in aquaculture.
显微镜观察溶血时间与血清皂苷浓度(2.5、5、12.5、25、50、125 mg/L)的相关性。分别测定了不同浓度的皂苷(2.5、5、12.5、25、50 mg/L)在20、4℃、不同时间下对大菱鲆血细胞的体外溶血率。为研究皂角皂苷对大菱鲆(Scophthalmus maximus)的毒性，将大菱鲆浸泡在不同浓度皂角皂苷的海水中，计算其中位致死浓度(LC50)。此外，测定了大比目鱼在0、5、25和45 mg/L皂苷海水溶液中浸泡后血清乳酸脱氢酶(LDH)活性。结果表明，大菱鲆红细胞溶血时间与皂苷浓度呈对数负相关(R2=0.98)，皂苷也能诱导大菱鲆白细胞溶血。50 mg/L浓度的皂苷在20℃作用5 min时溶血率为100%，而在4℃作用下溶血率仅为42.2%。皂苷对大菱鲆的24 h LC50为64.85 mg/L。本报告从细胞水平评价了皂苷对血细胞的溶血毒性，为其在水产养殖中的安全应用提供理论支持。
{"title":"Hemolysis of turbot(Scophthalmus maximus)blood cell with Quillaja saponaria saponin","authors":"Yujuan Wang, Xiuhua Wang, Wenbao Han, Rui Wang, Jie Huang","doi":"10.3724/SP.J.1231.2013.38523","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38523","url":null,"abstract":"The correlation between hemolysis time and concentration of saponin(2.5,5,12.5,25,50 and 125 mg/L)was analyzed via microscope observation.In vitro hemolysis rate of turbot blood cells in different concentrations of saponin(2.5,5,12.5,25 and 50 mg/L)at 20 and 4 ℃,for different time duration were tested respectively.To investigate the toxicity of saponin extracted from Quillaja saponaria to turbot(Scophthalmus maximus),median lethal concentration(LC50)was calculated by immersing the turbot in sea water with different concentrations of saponin.In addition,the lactic dehydrogenase(LDH)activities in serum of turbot after bathing in saponin seawater solution(0,5,25 and 45 mg/L)were analyzed.The results indicated that the correlation between hemolysis time of erythrocytes and saponin concentration showed a logarithmic negative correlation(R2=0.98),and that saponin could also induce hemolysis to leukocytes of turbot.Saponin in concentration of 50 mg/L could result in 100% hemolysis at 20 ℃ for 5 min,in contrast,only 42.2% hemolysis was observed at 4 ℃.The 24 h LC50 of saponin for S.maximus was 64.85 mg/L.In this report,from cell level,the evaluation of the toxicity of saponin to blood cells for hemolysis was made,which will theoretically support its safe application in aquaculture.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69967916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Simultaneous determination of 13 kinds of sulfonamides and trimethoprim residues of aquatic products by ASE-GPC-HPLC method","authors":"Jing Wang, Wuqi Shen, Meiqin Zhang, Yong Meng, Guanghong Wu","doi":"10.3724/SP.J.1231.2013.38507","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38507","url":null,"abstract":"","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69968231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38513
Quan Yang, Yongzhen Li, Peng Zhang, Yong Tang, Guobao Chen, Jun Zhang
Symplectoteuthis oualaniensis is a pelagic squid species widely distributed in the Indo-Pacific region,especially in the South China Sea,S.oualaniensis is the dominant species and therein there exists abundant biomass in the central and southern deep sea.It is necessary to carry out the exploitation and assessment of S.oualaniensis resources to promote the development of the fishery resources of the South China Sea.Due to the low population densities in natural conditions,resource survey by using squid jigging machine or pelagic trawl is ineffective.Although fisheries acoustics is a faster and more efficient way to assess the resources,because of insufficient data on biology and acoustic characteristics,it(when used alone)is not a perfect method.Therefore,the aim of this study is to explore a reasonable and effective assessment method and provide theoretic basis for reasonable utilization.This study proposes a model which combines light falling-net and acoustic survey by using a split-beam echo-sounder(Simrad EY60,120 kHz).Based on the acoustic data and biological data obtained from light falling-net conducted in the South China Sea from April to May in 2011,S.oualaniensis distribution,population structures,as well as method of acoustic survey were analyzed.The results showed that the effects of light trapping squids were obvious and the squid was mainly distributed at depths of 0-100 m.The average mantle length range was 10.4 to 14.2 cm,and the corresponding range of average target strength was-58 to-60.7 dB.Based on an analysis of collected data,the relationship TS=21.23logML-82.48 was obtained for S.oualaniensis at 120 kHz,with TS in dB and ML in centimeters.Our results indicated that this method is feasible and could provide important theoretical support for the squid resource assessment.
{"title":"Acoustic survey of the Symplectoteuthis oualaniensis resources in the South China Sea based on light falling-net","authors":"Quan Yang, Yongzhen Li, Peng Zhang, Yong Tang, Guobao Chen, Jun Zhang","doi":"10.3724/SP.J.1231.2013.38513","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38513","url":null,"abstract":"Symplectoteuthis oualaniensis is a pelagic squid species widely distributed in the Indo-Pacific region,especially in the South China Sea,S.oualaniensis is the dominant species and therein there exists abundant biomass in the central and southern deep sea.It is necessary to carry out the exploitation and assessment of S.oualaniensis resources to promote the development of the fishery resources of the South China Sea.Due to the low population densities in natural conditions,resource survey by using squid jigging machine or pelagic trawl is ineffective.Although fisheries acoustics is a faster and more efficient way to assess the resources,because of insufficient data on biology and acoustic characteristics,it(when used alone)is not a perfect method.Therefore,the aim of this study is to explore a reasonable and effective assessment method and provide theoretic basis for reasonable utilization.This study proposes a model which combines light falling-net and acoustic survey by using a split-beam echo-sounder(Simrad EY60,120 kHz).Based on the acoustic data and biological data obtained from light falling-net conducted in the South China Sea from April to May in 2011,S.oualaniensis distribution,population structures,as well as method of acoustic survey were analyzed.The results showed that the effects of light trapping squids were obvious and the squid was mainly distributed at depths of 0-100 m.The average mantle length range was 10.4 to 14.2 cm,and the corresponding range of average target strength was-58 to-60.7 dB.Based on an analysis of collected data,the relationship TS=21.23logML-82.48 was obtained for S.oualaniensis at 120 kHz,with TS in dB and ML in centimeters.Our results indicated that this method is feasible and could provide important theoretical support for the squid resource assessment.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69968280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38578
Hao Chen, Xugan Wu, Zhijun Liu, Yongxu Cheng
Progesterone is one of very important sex steroid hormones,which plays an important role during the ovarian development of crustaceans.In vertebrate,progesterone regulates the reproduction generally via progestin receptor(PR).Previous studies have shown PR is present in the ovary,hepatopancreas and nervous tissues for some crustacean species.The swimming crab,Portunus trituberculatus,is a commercially important fisheries resource and mariculture species in East Asian countries.The better understanding of reproductive mechanism would potentially benefit artificial propagation as well as fisheries management of P.trituberculatus.The present study was conducted to immunorecognize and immunolocalize PR in the ovary,hepatopancreas,mandibular organ,Y-organ,optic ganglia and thoracic ganglion mass of female P.trituberculatus via Western-blotting and immunohistochemistry.Then,the change and distribution of PR was also investigated in these tissues during the ovarian development of female P.trituberculatus.The results showed that PR with an apparent molecular weight of 100 ku was identified in the optic ganglia of female P.trituberculatus.By means of immunohistochemistry,PR was detected in the ovary,hepatopancreas,mandibular organ,optic ganglia and thoracic ganglion mass.During the ovarian development of female P.trituberculatus,the follicule cells were stained with strongly positive PR at each ovarian stage.As for the germinal cells,the strongly positive PR only exsited in the cytoplasm from the early ovary developmental stages,including stagesⅠ,Ⅱ and Ⅲ,while the strongly positive PR was stained in the nucleus of germinal cells during the stage Ⅲ to stage Ⅴ.For the female hepatopancreas,PR was mainly localized in the cytoplasm and nucleus of the fibrillar cell at all ovarian developmental stages,and PR was also present in the nucleus of stage Ⅲ resorptive cell.On the contrary,no positive PR-like substance was found in the other types of hepatopancreas cells,such as blister-like cell and embryonic cell during the ovarian development of female P.trituberculatus.As for the endocrine organ,PR was only localized in the cellular cytoplasm of the stage Ⅳ mandibular organ while positive PR-like substance was not found in the gland cell of Y-organ.Moreover,PR-like substance was also detected in the nervous tissues of female P.trituberculatus.As for the optic ganglia,PR was mainly localized in nerve cells from stage Ⅲ to stage Ⅴ.In addition,PR was also localized in the thoracic ganglion mass.Nerve cell and nerve medulla were stained positively with PR-anti-body from the ovarian stage Ⅰ to stage Ⅴ.In conclusion,these results suggest that progesterone not only can regulate the vitellogenesis and ovarian development directly via interacting with PR in ovary and hepatopancreas,but also may indirectly modulate ovarian development through the synthesis and secretion of other endocrine hormones.
{"title":"Immunorecognition and distribution of progestin receptor in the swimming crab Portunus trituberculatus during ovarian development","authors":"Hao Chen, Xugan Wu, Zhijun Liu, Yongxu Cheng","doi":"10.3724/SP.J.1231.2013.38578","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38578","url":null,"abstract":"Progesterone is one of very important sex steroid hormones,which plays an important role during the ovarian development of crustaceans.In vertebrate,progesterone regulates the reproduction generally via progestin receptor(PR).Previous studies have shown PR is present in the ovary,hepatopancreas and nervous tissues for some crustacean species.The swimming crab,Portunus trituberculatus,is a commercially important fisheries resource and mariculture species in East Asian countries.The better understanding of reproductive mechanism would potentially benefit artificial propagation as well as fisheries management of P.trituberculatus.The present study was conducted to immunorecognize and immunolocalize PR in the ovary,hepatopancreas,mandibular organ,Y-organ,optic ganglia and thoracic ganglion mass of female P.trituberculatus via Western-blotting and immunohistochemistry.Then,the change and distribution of PR was also investigated in these tissues during the ovarian development of female P.trituberculatus.The results showed that PR with an apparent molecular weight of 100 ku was identified in the optic ganglia of female P.trituberculatus.By means of immunohistochemistry,PR was detected in the ovary,hepatopancreas,mandibular organ,optic ganglia and thoracic ganglion mass.During the ovarian development of female P.trituberculatus,the follicule cells were stained with strongly positive PR at each ovarian stage.As for the germinal cells,the strongly positive PR only exsited in the cytoplasm from the early ovary developmental stages,including stagesⅠ,Ⅱ and Ⅲ,while the strongly positive PR was stained in the nucleus of germinal cells during the stage Ⅲ to stage Ⅴ.For the female hepatopancreas,PR was mainly localized in the cytoplasm and nucleus of the fibrillar cell at all ovarian developmental stages,and PR was also present in the nucleus of stage Ⅲ resorptive cell.On the contrary,no positive PR-like substance was found in the other types of hepatopancreas cells,such as blister-like cell and embryonic cell during the ovarian development of female P.trituberculatus.As for the endocrine organ,PR was only localized in the cellular cytoplasm of the stage Ⅳ mandibular organ while positive PR-like substance was not found in the gland cell of Y-organ.Moreover,PR-like substance was also detected in the nervous tissues of female P.trituberculatus.As for the optic ganglia,PR was mainly localized in nerve cells from stage Ⅲ to stage Ⅴ.In addition,PR was also localized in the thoracic ganglion mass.Nerve cell and nerve medulla were stained positively with PR-anti-body from the ovarian stage Ⅰ to stage Ⅴ.In conclusion,these results suggest that progesterone not only can regulate the vitellogenesis and ovarian development directly via interacting with PR in ovary and hepatopancreas,but also may indirectly modulate ovarian development through the synthesis and secretion of other endocrine hormones.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69968529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38629
Cai-xia Yang, Yun Li, Chongming Wang, Peng Qu, Jie Huang
Acute viral necrosis virus(AVNV),which caused mass mortality of scallops Chlamys farreri,is a DNA virus. In the present study,the detection of AVNV was conducted on culture scallops from different culture modes and seeds to search appropriate culture modes and seeds which could reduce mortality of culture scallops. Ten adult scallops were sampled regularly from scallop single-cultured system( Liuqing River,Qingdao) and scallop-kelp co-cultured system( Sanggou Bay,Rongcheng) per month,both contained scallops originating from wild-seed and artificial-seed(March 2010- April 2011,total 480 scallops were sampled). The scallops were taken to the lab at low tempreture. Mantle tissue DNA was extracted and used as the template for nest-PCR to evaluate infection of AVNV. The results show ed that the infection rates of AVNV for cultured scallops from wild-seed and artificial-seed were 21.1% and 18.9% in Liuqing River, while in Sanggou Bay the infection rates were 11.1% and 5.6%. In both sampling sites,high infection rates were detected in July and August. Highest infection rate in Liuqing River reached 80%,while in Sanggou Bay that was only 40%. The present study indicated that scallop-kelp co-cultured system and artificial-seed could effectively decrease the infection rate of AVNV,which could be useful methods to control occurrence of scallop disease.
{"title":"Comparative analysis of scallop Chlamys farreri infected by acute viral necrosis virus( AVNV) in two areas","authors":"Cai-xia Yang, Yun Li, Chongming Wang, Peng Qu, Jie Huang","doi":"10.3724/SP.J.1231.2013.38629","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38629","url":null,"abstract":"Acute viral necrosis virus(AVNV),which caused mass mortality of scallops Chlamys farreri,is a DNA virus. In the present study,the detection of AVNV was conducted on culture scallops from different culture modes and seeds to search appropriate culture modes and seeds which could reduce mortality of culture scallops. Ten adult scallops were sampled regularly from scallop single-cultured system( Liuqing River,Qingdao) and scallop-kelp co-cultured system( Sanggou Bay,Rongcheng) per month,both contained scallops originating from wild-seed and artificial-seed(March 2010- April 2011,total 480 scallops were sampled). The scallops were taken to the lab at low tempreture. Mantle tissue DNA was extracted and used as the template for nest-PCR to evaluate infection of AVNV. The results show ed that the infection rates of AVNV for cultured scallops from wild-seed and artificial-seed were 21.1% and 18.9% in Liuqing River, while in Sanggou Bay the infection rates were 11.1% and 5.6%. In both sampling sites,high infection rates were detected in July and August. Highest infection rate in Liuqing River reached 80%,while in Sanggou Bay that was only 40%. The present study indicated that scallop-kelp co-cultured system and artificial-seed could effectively decrease the infection rate of AVNV,which could be useful methods to control occurrence of scallop disease.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69969682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38668
Lin Li, Xinghong Yan
The specific inhibitors of cytoskeleton and histochemical stain were used to investigate formation of cell polarization during the early development of conchospores in Pyropia haitanensis. Polarized conchospores w ere observed tw ice: Anterior-posterior axis is formed in migrating conchospores. After migration,conchospores adhere to the substratum and then apical-basal axis is established. Meanw hile,composition of mucilage around conchospores w as studied,and acidic polysaccharides and sulfated polysaccharides w ere found at the rear end in migrating conchospores and basal portion of up-righted conchospores. These results indicate that mucilage is involved in formation and maintenance of the cell polarization in conchospores. Inhibitors of cytoskeleton prevented the released conchospores from germinating through disturbing the anterior-posterior axis formed in migrating conchospores. It w as found that they also affected the establishment of apical-basal axis for abnormal germinations in the inhibitors-treated migrated conchospores. These results indicate that cytoskeleton and mucilage are involved constantly in tw ice cell polarization formation of conchospores in P. haitanensis.
{"title":"Cytoskeleton and mucilage involved in the cell polarization of conchospores in Pyropia haitanensis","authors":"Lin Li, Xinghong Yan","doi":"10.3724/SP.J.1231.2013.38668","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38668","url":null,"abstract":"The specific inhibitors of cytoskeleton and histochemical stain were used to investigate formation of cell polarization during the early development of conchospores in Pyropia haitanensis. Polarized conchospores w ere observed tw ice: Anterior-posterior axis is formed in migrating conchospores. After migration,conchospores adhere to the substratum and then apical-basal axis is established. Meanw hile,composition of mucilage around conchospores w as studied,and acidic polysaccharides and sulfated polysaccharides w ere found at the rear end in migrating conchospores and basal portion of up-righted conchospores. These results indicate that mucilage is involved in formation and maintenance of the cell polarization in conchospores. Inhibitors of cytoskeleton prevented the released conchospores from germinating through disturbing the anterior-posterior axis formed in migrating conchospores. It w as found that they also affected the establishment of apical-basal axis for abnormal germinations in the inhibitors-treated migrated conchospores. These results indicate that cytoskeleton and mucilage are involved constantly in tw ice cell polarization formation of conchospores in P. haitanensis.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69969795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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