Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38752
Bao-yan Zhang, Hai-yang Yu, Yu-dong Cheng, Yinzhe Jin
In order to improve the rehydration rate and rehydration effect,the dried tilapia fillets( moisture content decreased to 0. 100 0( g / g d. b.)( dry basis)) w ere hydrated by microw ave. Tilapia fillets w ere put into the container made of acrylic materials w ith different sizes and shapes. The effect of container shape, container size,and salt content on the microw ave rehydration w as investigated. The result show s that the moistureed content increased gradually w ith the rehydration time extending,reached a maximum value and maintain stability after 250- 300 s. The moisture content of sample after rehydration process did not reach the initial moisture content( 3. 859 0( g / g d. b.)). It is show n that the rehydration process is irreversible, cell dehydration and damaged organizational structure during the drying process cause the rehydration ability of sample to decrease. In this study,the larger size of the container has a better rehydration effect. Compared w ith cubic container,cylindrical container is more advantageous to the microw ave rehydration for Tilapia fillets. The dielectric loss rate of soup material increased w ith the increasing of NaCl contents,so that the rehydration rate increased. In summary,rehydration time by microw ave is about 240 s,w hich saves about 2 / 3 time compared w ith the traditional w ater bath( about 700 s). Moreover,some models such as Peleg and Weibull model w ere used to fit the rehydration process. It w as found that Peleg model has a higher fitness for the experiment data than Weibull model. Peleg model w as more suitable for describing the rehydration process of tilapia fillets.
为提高复水率和复水效果，将罗非鱼干鱼片含水率降至0。将罗非鱼鱼片放入不同大小形状的亚克力材料制成的容器中。研究了容器形状、容器尺寸和含盐量对微波复水化的影响。结果表明，随着复水时间的延长，水分含量逐渐增加，在250 ~ 300 s后达到最大值并保持稳定。复水化处理后的样品含水率未达到初始含水率(3。859 (g / g d. b.))。结果表明，再水化过程是不可逆的，干燥过程中细胞脱水和组织结构破坏导致样品的再水化能力下降。在本研究中，容器尺寸越大，复水效果越好。与立方容器相比，圆柱形容器更有利于罗非鱼鱼片的微波复水化。随着NaCl含量的增加，汤料的介电损耗率增大，从而使复水化率增大。综上所述，微波复水时间约为240 s，与传统水浴(约700 s)相比，可节省约2 / 3的时间，并采用Peleg和Weibull模型等模型对复水过程进行拟合。结果表明，Peleg模型比Weibull模型对实验数据的拟合度更高。Peleg模型更适合描述罗非鱼鱼片的复水化过程。
{"title":"Effects of packaging size and shape on the microwave rehydration characteristics of tilapia fillets","authors":"Bao-yan Zhang, Hai-yang Yu, Yu-dong Cheng, Yinzhe Jin","doi":"10.3724/SP.J.1231.2013.38752","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38752","url":null,"abstract":"In order to improve the rehydration rate and rehydration effect,the dried tilapia fillets( moisture content decreased to 0. 100 0( g / g d. b.)( dry basis)) w ere hydrated by microw ave. Tilapia fillets w ere put into the container made of acrylic materials w ith different sizes and shapes. The effect of container shape, container size,and salt content on the microw ave rehydration w as investigated. The result show s that the moistureed content increased gradually w ith the rehydration time extending,reached a maximum value and maintain stability after 250- 300 s. The moisture content of sample after rehydration process did not reach the initial moisture content( 3. 859 0( g / g d. b.)). It is show n that the rehydration process is irreversible, cell dehydration and damaged organizational structure during the drying process cause the rehydration ability of sample to decrease. In this study,the larger size of the container has a better rehydration effect. Compared w ith cubic container,cylindrical container is more advantageous to the microw ave rehydration for Tilapia fillets. The dielectric loss rate of soup material increased w ith the increasing of NaCl contents,so that the rehydration rate increased. In summary,rehydration time by microw ave is about 240 s,w hich saves about 2 / 3 time compared w ith the traditional w ater bath( about 700 s). Moreover,some models such as Peleg and Weibull model w ere used to fit the rehydration process. It w as found that Peleg model has a higher fitness for the experiment data than Weibull model. Peleg model w as more suitable for describing the rehydration process of tilapia fillets.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"1854"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69970385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38743
Y. Liao, Bo Liu, M. Ren, Honghong Cui, Jun Xie, X. Ge
A 10-week feeding trial was conducted to investigate effects of lysine on growth,physiological and biochemical indexes of blood and essential amino acids of plasma in juvenile Megalobrama amblycephala. Six isonitrogenous and isoenergetic semi-purified diets w ere formulated to contain graded dietary lysine levels,and the values w ere 1. 29%,1. 71%,2. 09%,2. 48%,2. 88% and 3. 27%,respectively. At the initiation of experiment,fish[( 3. 34 ± 0. 03) g]w ere randomly chosen and sorted into eighteen cages w ith 30 fish per cage. Each experimental diet w as randomly assigned to triplicate cages in a completely randomized design. At the end of feeding trial,final body w eight( FBW),w eight gain rate( WRG),specific growth rate( SGR),Nitrogen retention efficiency( NRE),protein productive value( PPV)and protein efficiency ratio( PER) of fish fed diet containing 2. 48% lysine w ere significantly higher than those fish fed 1. 29% lysine diet w hile feed conversion ratio( FCR) show ed an adverse tendency,but no significant difference w as observed in survival rate( SR) of fish fed different dietary methionine levels.Increased crude protein and reduced viscerosomatic ratio( VR) w ere observed in fish fed 2. 48% lysine diet.Ash of w hole body of fish fed 2. 88% and 3. 27% lysine diet w as higher than those fish fed 1. 29% lysine diet. Alanine aminotransferase( ALT) and total protein( TP) contents w ere significantly improved w ith increasing lysine levels up to 3. 27%. Urea content in plasma of fish fed 2. 94% lysine diet w as low er than those fish fed 1. 29% lysine diet. Fish fed the 2. 88% lysine diet demonstrated a significant improvement in plasma lysine content and total essential amino acid except for Leu and Val. On the basis of SGR and PPV,the optimum dietary lysine requirement of juvenile M. amblycephala w as estimated to be 2. 36% of diet( 6. 94% of protein) and 2. 22% of diet( 6. 53% of protein),respectively,using broken-line regression analysis.
{"title":"Effects of lysine on growth,physiological and biochemical indexes of blood and essential amino acids of serum in juvenile blunt snout bream(Megalobrama amblycephala)","authors":"Y. Liao, Bo Liu, M. Ren, Honghong Cui, Jun Xie, X. Ge","doi":"10.3724/SP.J.1231.2013.38743","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38743","url":null,"abstract":"A 10-week feeding trial was conducted to investigate effects of lysine on growth,physiological and biochemical indexes of blood and essential amino acids of plasma in juvenile Megalobrama amblycephala. Six isonitrogenous and isoenergetic semi-purified diets w ere formulated to contain graded dietary lysine levels,and the values w ere 1. 29%,1. 71%,2. 09%,2. 48%,2. 88% and 3. 27%,respectively. At the initiation of experiment,fish[( 3. 34 ± 0. 03) g]w ere randomly chosen and sorted into eighteen cages w ith 30 fish per cage. Each experimental diet w as randomly assigned to triplicate cages in a completely randomized design. At the end of feeding trial,final body w eight( FBW),w eight gain rate( WRG),specific growth rate( SGR),Nitrogen retention efficiency( NRE),protein productive value( PPV)and protein efficiency ratio( PER) of fish fed diet containing 2. 48% lysine w ere significantly higher than those fish fed 1. 29% lysine diet w hile feed conversion ratio( FCR) show ed an adverse tendency,but no significant difference w as observed in survival rate( SR) of fish fed different dietary methionine levels.Increased crude protein and reduced viscerosomatic ratio( VR) w ere observed in fish fed 2. 48% lysine diet.Ash of w hole body of fish fed 2. 88% and 3. 27% lysine diet w as higher than those fish fed 1. 29% lysine diet. Alanine aminotransferase( ALT) and total protein( TP) contents w ere significantly improved w ith increasing lysine levels up to 3. 27%. Urea content in plasma of fish fed 2. 94% lysine diet w as low er than those fish fed 1. 29% lysine diet. Fish fed the 2. 88% lysine diet demonstrated a significant improvement in plasma lysine content and total essential amino acid except for Leu and Val. On the basis of SGR and PPV,the optimum dietary lysine requirement of juvenile M. amblycephala w as estimated to be 2. 36% of diet( 6. 94% of protein) and 2. 22% of diet( 6. 53% of protein),respectively,using broken-line regression analysis.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"1716"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69970501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38746
Zhigang Liu, X. Ke, Mai-xin Lu, Huaping Zhu, F. Gao
In order to understand the influence and mechanism of temperature on the virulence of Streptococcus agalactiae,the effects of culture temperature on the virulence related parameters of S. agalactiae including bacteria grow th,adhesion,invasion,expression of virulence genes and mortality rates of tilapia w ere studied. The results show ed that the grow th speeds of S. agalactiae at different temperatures( 25-40 ℃) were different; 37 ℃ was the optimum growth temperature and the slowest growth speed appeared at 25 ℃. The adhesive capacity of S. agalactiae reached the peak at 37 ℃ and decreased at 40 ℃. There w as no significant difference betw een the absorbance( OD 590 nm) of adhesive S. agalactiae incubated in the range of 25- 34 ℃. The number of colony forming units in the brain of tilapia infected artificially w ith S. agalactiae show ed a temperature-dependent grow th pattern. The number of colony forming units increased along w ith the increase of culture temperature and reached the peak at 37 ℃,and then decreased at 40 ℃. The number of colony forming units w as positively associated w ith its mortality rate. The expression of virulence genes w as also influenced by culture temperature. The expression level of hly and cfb genes show ed a temperature-dependent expression pattern similar to the number of colony forming units. The expression of hly and cfb genes significantly increased and reached the peak at 34 ℃ and 37 ℃,respectively,and then dropped rapidly w hile the culture temperature continued to rise. The expression of sip gene w as minimally influenced by culture temperature w hile the expression of scpB gene decreased along w ith the increase of the culture temperature. The mortality rates of tilapia infected w ith S. agalactiae cultured at different temperatures show ed temperature-dependence. The mortality rate increased along w ith the increase of culture temperature and peaked 66. 67% ± 6. 67% at 37 ℃,and then slightly decreased at 40 ℃. In conclusion,the culture temperature influenced the grow th,adhesion,invasion and expression of some virulence genes of S. agalactiae,w hich may contribute to its virulence.
{"title":"Effect of temperature on the virulence of Streptococcus agalactiae from Nile tilapia(Oreochromis niloticus)","authors":"Zhigang Liu, X. Ke, Mai-xin Lu, Huaping Zhu, F. Gao","doi":"10.3724/SP.J.1231.2013.38746","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38746","url":null,"abstract":"In order to understand the influence and mechanism of temperature on the virulence of Streptococcus agalactiae,the effects of culture temperature on the virulence related parameters of S. agalactiae including bacteria grow th,adhesion,invasion,expression of virulence genes and mortality rates of tilapia w ere studied. The results show ed that the grow th speeds of S. agalactiae at different temperatures( 25-40 ℃) were different; 37 ℃ was the optimum growth temperature and the slowest growth speed appeared at 25 ℃. The adhesive capacity of S. agalactiae reached the peak at 37 ℃ and decreased at 40 ℃. There w as no significant difference betw een the absorbance( OD 590 nm) of adhesive S. agalactiae incubated in the range of 25- 34 ℃. The number of colony forming units in the brain of tilapia infected artificially w ith S. agalactiae show ed a temperature-dependent grow th pattern. The number of colony forming units increased along w ith the increase of culture temperature and reached the peak at 37 ℃,and then decreased at 40 ℃. The number of colony forming units w as positively associated w ith its mortality rate. The expression of virulence genes w as also influenced by culture temperature. The expression level of hly and cfb genes show ed a temperature-dependent expression pattern similar to the number of colony forming units. The expression of hly and cfb genes significantly increased and reached the peak at 34 ℃ and 37 ℃,respectively,and then dropped rapidly w hile the culture temperature continued to rise. The expression of sip gene w as minimally influenced by culture temperature w hile the expression of scpB gene decreased along w ith the increase of the culture temperature. The mortality rates of tilapia infected w ith S. agalactiae cultured at different temperatures show ed temperature-dependence. The mortality rate increased along w ith the increase of culture temperature and peaked 66. 67% ± 6. 67% at 37 ℃,and then slightly decreased at 40 ℃. In conclusion,the culture temperature influenced the grow th,adhesion,invasion and expression of some virulence genes of S. agalactiae,w hich may contribute to its virulence.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"51 1","pages":"1733"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69970613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38204
Guoping Zhu, Chaohua Zhang, Wenhong Cao, Ji Hongwu
Shrimp head is susceptible to autolysis under certain conditions,the protein in it is degraded into soluble protein,peptides and amino acids,and some peptides are active peptides which can inhibit the ACE enzyme activity.At present,many ACE inhibitory peptides derived from food protein have been developed.In the present study,two ACE inhibitory peptides(Tyr-Pro and Leu-Pro/Ile-Pro)were highly purified from the shrimp head(Litopenaeus vannamei)autolysate by extra fine membrane and a series of column chromatographies.In the first autolysis solution of shrimp head was consecutively extracted through extra fine membrane with molecular weight cut-offs(MWCO)at 8,5,3 ku,respectively.The active results shown that filtrate through MWCO at 3 000 u had the highest ACE inhibitory activity.The crude filtrate through MWCO at 3 ku was purified by Sephadex G-25 gel chromatography,SP Sephadex C-25 anion-exchange chromatography as well as Sephadex G-15 gel chromatography,respectively.After that,the ACE inhibitory activity of purified filtrate almost increased by 8 times(IC50=0.19 mg/mL)that of crude filtrate.The high active collected fraction from Sephadex G-15 gel chromatography was carried out by RP-HPLC(High-Performance Liquid Chromatography)twice for the further purification and two kinds of dipeptide were obtained,and the identification of dipeptide by mass spectra showed that they were Tyr-Pro and Leu-Pro/Ile-Pro,and the molecular weight was 279 u and 229 u,respectively.
{"title":"Isolation and identification of ACE inhibitory peptides from the autolysis product of shrimp head(Litopenaeus vannamei)","authors":"Guoping Zhu, Chaohua Zhang, Wenhong Cao, Ji Hongwu","doi":"10.3724/SP.J.1231.2013.38204","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38204","url":null,"abstract":"Shrimp head is susceptible to autolysis under certain conditions,the protein in it is degraded into soluble protein,peptides and amino acids,and some peptides are active peptides which can inhibit the ACE enzyme activity.At present,many ACE inhibitory peptides derived from food protein have been developed.In the present study,two ACE inhibitory peptides(Tyr-Pro and Leu-Pro/Ile-Pro)were highly purified from the shrimp head(Litopenaeus vannamei)autolysate by extra fine membrane and a series of column chromatographies.In the first autolysis solution of shrimp head was consecutively extracted through extra fine membrane with molecular weight cut-offs(MWCO)at 8,5,3 ku,respectively.The active results shown that filtrate through MWCO at 3 000 u had the highest ACE inhibitory activity.The crude filtrate through MWCO at 3 ku was purified by Sephadex G-25 gel chromatography,SP Sephadex C-25 anion-exchange chromatography as well as Sephadex G-15 gel chromatography,respectively.After that,the ACE inhibitory activity of purified filtrate almost increased by 8 times(IC50=0.19 mg/mL)that of crude filtrate.The high active collected fraction from Sephadex G-15 gel chromatography was carried out by RP-HPLC(High-Performance Liquid Chromatography)twice for the further purification and two kinds of dipeptide were obtained,and the identification of dipeptide by mass spectra showed that they were Tyr-Pro and Leu-Pro/Ile-Pro,and the molecular weight was 279 u and 229 u,respectively.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"631"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69963949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38261
Li Xupeng, Meng Xianhong, Kong Jie, Cao Baoxiang, L. Ning, Luo Kun, Liu Weiya
White spot syndrome virus(WSSV)is one kind of destructive virus affecting shrimp industry in the world.Output of Fenneropenaeus chinensis which once was a staple species has been greatly affected by outbreak of WSSV since 1993 in China.To produce WSSV-resistance shrimp strains is an important approach to revive the shrimp industry.To select survived F.chinensis as broodstock via WSSV challenge test can increase the efficiency of breeding.However,mortality of shrimps could reach nearly 100% once they were infected with WSSV,which increases the difficulty for shrimp breeding.In this study for the purpose to reveal effect of different WSSV amount on F.chinensis,juvenile F.chinensis were infected with a certain amount of WSSV individually and the survival time and survival rate were observed.The results showed that survival time of shrimps infected with 5.2×108 copies,1.0×109 copies and 2.1×109 copies of WSSV respectively was 389.3,323.3 and 187.3 h and significant different(P0.01).The cumulative mortalities of all the shrimps finally reached 100%.The survival time increased while shrimps were fed with less WSSV bait.For the purpose to reveal the effect of diets on the ability for disease resistance,shrimps infected with WSSV were fed with different diets of live brine shrimps,commercial bait and fresh clam.It was showed that the survival time of infected shrimps fed with live brine shrimps,fresh clam and commercial bait respectively was 281.7,173.9 and 164.9 h.The survival time of infected shrimp fed with live brine shrimps was significantly higher than that fed by commercial bait and fresh clam respectively(P0.01).There was no significant difference of survival time between infected shrimps fed with commercial bait and fresh clam(P0.05).Cumulative mortalities of all the groups of infected shrimps that fed with different diets were 100%.Live brine shrimp is better than commercial bait and fresh clam for the purpose of enhancing shrimp WSSV resistance ability.
{"title":"Effects on survival time of Fenneropenaeus chinensis challenged by different doses of WSSV and diets","authors":"Li Xupeng, Meng Xianhong, Kong Jie, Cao Baoxiang, L. Ning, Luo Kun, Liu Weiya","doi":"10.3724/SP.J.1231.2013.38261","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38261","url":null,"abstract":"White spot syndrome virus(WSSV)is one kind of destructive virus affecting shrimp industry in the world.Output of Fenneropenaeus chinensis which once was a staple species has been greatly affected by outbreak of WSSV since 1993 in China.To produce WSSV-resistance shrimp strains is an important approach to revive the shrimp industry.To select survived F.chinensis as broodstock via WSSV challenge test can increase the efficiency of breeding.However,mortality of shrimps could reach nearly 100% once they were infected with WSSV,which increases the difficulty for shrimp breeding.In this study for the purpose to reveal effect of different WSSV amount on F.chinensis,juvenile F.chinensis were infected with a certain amount of WSSV individually and the survival time and survival rate were observed.The results showed that survival time of shrimps infected with 5.2×108 copies,1.0×109 copies and 2.1×109 copies of WSSV respectively was 389.3,323.3 and 187.3 h and significant different(P0.01).The cumulative mortalities of all the shrimps finally reached 100%.The survival time increased while shrimps were fed with less WSSV bait.For the purpose to reveal the effect of diets on the ability for disease resistance,shrimps infected with WSSV were fed with different diets of live brine shrimps,commercial bait and fresh clam.It was showed that the survival time of infected shrimps fed with live brine shrimps,fresh clam and commercial bait respectively was 281.7,173.9 and 164.9 h.The survival time of infected shrimp fed with live brine shrimps was significantly higher than that fed by commercial bait and fresh clam respectively(P0.01).There was no significant difference of survival time between infected shrimps fed with commercial bait and fresh clam(P0.05).Cumulative mortalities of all the groups of infected shrimps that fed with different diets were 100%.Live brine shrimp is better than commercial bait and fresh clam for the purpose of enhancing shrimp WSSV resistance ability.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"263-268"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69964411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.37994
Yunhui Pu, X. Xu, Huan Gao, Zhao-pu Liu, B. Yan
Exopalaemon carinicauda is one of the marine aquaculture species with high economic value in China.This paper studied the effects of cryopreservation(-196 ℃)on the enzyme activity(SDH,LDH,Na+K+-ATP,SOD,CAT,GR and Acrosin)in E.carinicauda spermatozoa,in order to provide a theoretical basis for cryopreservation effect on E.carinicauda spermatozoa,and lay a foundation for artificial propagation of E.carinicauda.We set up a control group(without cryoprotectant),and three experimental groups(DMSO(V/V)10%,12.5%,15%),detected the enzyme activities on day 0,1,3,5,7,15.The result showed that,after cryopreservation,in addition to GR,the other enzyme activities decreased significantly(P0.05),with the biggest drop in the control group.GR activity significantly increased in 7 days,and the control group was significantly higher than the experimental group(P0.05),declining again in 15 days.The group of 15% DMSO measured enzyme activities were higher than other groups,indicating that 15% DMSO had protective effect on enzyme of spermatozoa.15 days later,under the condition of 15% DMSO,the activities of SDH,LDH and Na+/K+-ATPase decreased from(28.500±1.453)U/mL,(1290.836±27.603)U/L and(2.605±0.232)μmol/(mg·h)to(15.300±0.950)U/mL,(363.713±13.943)U/L and(0.542±0.186)μmol/(mg·h)respectively;the activities of SOD and CAT decreased from(106.497±7.217)U/mL,(383.632±4.731)U/g to(17.036±0.321)U/mL and(166.940±1.910)U/g respectively;the activities of acrosin decreased from(3.521±0.010)μIU/106 to(1.212±0.043)μIU/106;but the activities of GR increased from(217.042±6.962)U/L to(302.787±24.558)U/L.From the decline range,cryopreservation had the biggest influence on SOD,followed by Na+/K+-ATPase.
{"title":"Effects of cryopreservation on enzyme activity of Exopalaemon carinicauda spermatozoa","authors":"Yunhui Pu, X. Xu, Huan Gao, Zhao-pu Liu, B. Yan","doi":"10.3724/SP.J.1231.2013.37994","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.37994","url":null,"abstract":"Exopalaemon carinicauda is one of the marine aquaculture species with high economic value in China.This paper studied the effects of cryopreservation(-196 ℃)on the enzyme activity(SDH,LDH,Na+K+-ATP,SOD,CAT,GR and Acrosin)in E.carinicauda spermatozoa,in order to provide a theoretical basis for cryopreservation effect on E.carinicauda spermatozoa,and lay a foundation for artificial propagation of E.carinicauda.We set up a control group(without cryoprotectant),and three experimental groups(DMSO(V/V)10%,12.5%,15%),detected the enzyme activities on day 0,1,3,5,7,15.The result showed that,after cryopreservation,in addition to GR,the other enzyme activities decreased significantly(P0.05),with the biggest drop in the control group.GR activity significantly increased in 7 days,and the control group was significantly higher than the experimental group(P0.05),declining again in 15 days.The group of 15% DMSO measured enzyme activities were higher than other groups,indicating that 15% DMSO had protective effect on enzyme of spermatozoa.15 days later,under the condition of 15% DMSO,the activities of SDH,LDH and Na+/K+-ATPase decreased from(28.500±1.453)U/mL,(1290.836±27.603)U/L and(2.605±0.232)μmol/(mg·h)to(15.300±0.950)U/mL,(363.713±13.943)U/L and(0.542±0.186)μmol/(mg·h)respectively;the activities of SOD and CAT decreased from(106.497±7.217)U/mL,(383.632±4.731)U/g to(17.036±0.321)U/mL and(166.940±1.910)U/g respectively;the activities of acrosin decreased from(3.521±0.010)μIU/106 to(1.212±0.043)μIU/106;but the activities of GR increased from(217.042±6.962)U/L to(302.787±24.558)U/L.From the decline range,cryopreservation had the biggest influence on SOD,followed by Na+/K+-ATPase.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"37 1","pages":"101"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69962326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.37789
C. Song, Xi-wu Yan, Shilei Sang, Z. Huo, Qing Liu
13 half-sib families and 39 full-sib families of Mytilus edulis were established with the balance nest design in which each male mated with three females and the artificial fertilization technique.The shell height and length of each family were measured in 2,3,5,6 months.The heritability of the growth was estimated using the full-sib families theories.The results show that the maternal genetic variance component estimates are all significantly greater than those of paternal ones of each growth trait(P0.05).The female genetic variance components have significant maternal effects(P0.05),suggesting that the large non-additive genetic effects could not be differentiated from the available data,so the heritability of paternal half-sib families estimates in the narrow sense were precise unbiased estimator.The shell height heritability of paternal half-sib families estimates are 0.29,0.85,0.84,0.87 respectively.The shell length heritability are 0.27,0.87,0.86,0.85 respectively,during 2,3,5,6 months.There were significant additive genetic variance of the growth and different heritability of shell height and shell length,indicating that there is a great potential genetic improvement for the growth of M.edulis using the selective breeding.
{"title":"Estimates for the heritability of growth of Mytilus edulis","authors":"C. Song, Xi-wu Yan, Shilei Sang, Z. Huo, Qing Liu","doi":"10.3724/SP.J.1231.2013.37789","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.37789","url":null,"abstract":"13 half-sib families and 39 full-sib families of Mytilus edulis were established with the balance nest design in which each male mated with three females and the artificial fertilization technique.The shell height and length of each family were measured in 2,3,5,6 months.The heritability of the growth was estimated using the full-sib families theories.The results show that the maternal genetic variance component estimates are all significantly greater than those of paternal ones of each growth trait(P0.05).The female genetic variance components have significant maternal effects(P0.05),suggesting that the large non-additive genetic effects could not be differentiated from the available data,so the heritability of paternal half-sib families estimates in the narrow sense were precise unbiased estimator.The shell height heritability of paternal half-sib families estimates are 0.29,0.85,0.84,0.87 respectively.The shell length heritability are 0.27,0.87,0.86,0.85 respectively,during 2,3,5,6 months.There were significant additive genetic variance of the growth and different heritability of shell height and shell length,indicating that there is a great potential genetic improvement for the growth of M.edulis using the selective breeding.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"37 1","pages":"201"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69962392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The growth,development and sexual maturity of pond-reared Penaeus monodon","authors":"Jianhua Huang, Qibin Yang, Ziming Ma, Xu Chen, F. Zhou, Weigeng Wen, Shi-gui Jiang","doi":"10.3724/SP.J.1231.2013.38174","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38174","url":null,"abstract":"","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"37 1","pages":"397"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69963334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38191
W. Ting, Xu Yan, Xie Chaotian, J. Dehua, Chen Chang-sheng
Porphyra haitanensis is one of the most important economic marine crops of China.For any cultivar,the correct identification of species or forma of the cultivated strains is necessary to ensure a well-bred cultivation and good production quality.However,because the gametophytic blade of Porphyra is morphologically simple and marked variations can occur as environmental conditions change,it is very difficult to precisely identify the species or forma of cultivated strains based only on their morphological characteristics.With new advances in molecular biology,molecular markers and DNA fingerprinting techniques have become routine for the identification and classification of many crops,including seaweeds.The strain of "Z-26" of P.haitanensis was selected by the laboratory of germplasm improvement and the application of P.haitanensis in Jimei University which has the characters of high-temperature tolerance and high yield,and it has been widely cultivated in south China.In order to construct the technology of variety identification for "Z-26",firstly,300 primers of RAPD were used to scan the specific markers of 6 new strains of P.haitanensis and 9 specific RAPD markers of "Z-26" were selected.After cloning and sequencing,two specific RAPD markers of "Z-26" were transformed into the SCAR markers successfully,the length of the 2 SCAR markers was 540 and 242 bp,respectively.Secondly,after verification by 4 different experiments,we can affirm that the 2 SCAR markers were the specific and stable markers of "Z-26".At last,based on the 2 SCAR markers,after optimization of experimental conditions,the technology of multiplex PCR which was used to identify the variety of "Z-26" was constructed.The result supplied a simple,fast and reliable technigue for variety identification of "Z-26".
{"title":"Construction of multiplex PCR in variety identification of Porphyra haitanensis \"Z-26\" based on SCAR markers.","authors":"W. Ting, Xu Yan, Xie Chaotian, J. Dehua, Chen Chang-sheng","doi":"10.3724/SP.J.1231.2013.38191","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38191","url":null,"abstract":"Porphyra haitanensis is one of the most important economic marine crops of China.For any cultivar,the correct identification of species or forma of the cultivated strains is necessary to ensure a well-bred cultivation and good production quality.However,because the gametophytic blade of Porphyra is morphologically simple and marked variations can occur as environmental conditions change,it is very difficult to precisely identify the species or forma of cultivated strains based only on their morphological characteristics.With new advances in molecular biology,molecular markers and DNA fingerprinting techniques have become routine for the identification and classification of many crops,including seaweeds.The strain of \"Z-26\" of P.haitanensis was selected by the laboratory of germplasm improvement and the application of P.haitanensis in Jimei University which has the characters of high-temperature tolerance and high yield,and it has been widely cultivated in south China.In order to construct the technology of variety identification for \"Z-26\",firstly,300 primers of RAPD were used to scan the specific markers of 6 new strains of P.haitanensis and 9 specific RAPD markers of \"Z-26\" were selected.After cloning and sequencing,two specific RAPD markers of \"Z-26\" were transformed into the SCAR markers successfully,the length of the 2 SCAR markers was 540 and 242 bp,respectively.Secondly,after verification by 4 different experiments,we can affirm that the 2 SCAR markers were the specific and stable markers of \"Z-26\".At last,based on the 2 SCAR markers,after optimization of experimental conditions,the technology of multiplex PCR which was used to identify the variety of \"Z-26\" was constructed.The result supplied a simple,fast and reliable technigue for variety identification of \"Z-26\".","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"37 1","pages":"688-695"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69963508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38170
Hang-Jun Wang, X. Ye, Yuan-yuan Tian, Li-li Zhang, Guocheng Deng
{"title":"Analysis of function and immunogenicity of GCRV-GD108 VP5","authors":"Hang-Jun Wang, X. Ye, Yuan-yuan Tian, Li-li Zhang, Guocheng Deng","doi":"10.3724/SP.J.1231.2013.38170","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38170","url":null,"abstract":"","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"37 1","pages":"109"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69963767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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