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Effects of water temperature and fish size on growth and nitrogen budget of cobia(Rachycentron canadum) 水温和鱼的大小对军曹鱼生长和氮收支的影响
Q4 Environmental Science Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1231.2013.38597
Lihua Sun, Haoru Chen
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引用次数: 0
Correlation analysis of major morphometric traits and body weight of selective group at different month ages of Trachinotus ovatus 选择组不同月龄卵形沙眼主要形态计量性状与体重的相关性分析
Q4 Environmental Science Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1231.2013.38599
Ou Youjun, Jifin Lei, Li Jiaer, Fan Chunyan, W. Gang
To investigate the effect of major morphometric traits of selective group of Trachinotus ovatus at different month ages on body weight,2 635 specimens at 1-month-old stage,4-month-old stage,7-month-old stage,10-month-old stage and 13-month-old stage were measured respectively in terms of total length(x1),body length(x2),body height(x3)and weight(y).Correlation coefficients,path coefficients and determination coefficients of each trait to body weight were calculated through path analysis and multiple linear regression.Effects of each morphometric trait on body weight were also dissected and then the optimal regression equation was established.The results showed that the correlations among 4 traits ranged from 0.017 to 0.960,and all reached significant level(P0.01)at different stages except that at 4-month-old stage.At 1-month-old stage and 4-month-old stage,the trait with strongest direct effect on body weight was the total length,but that at other stages was the body height.The result of determinant coefficient analysis was consistent with that of path analysis,and the fitness of equations at different stages was good(86.9%-90.6%).It indicated that the major morphometric traits affecting body weight were found and traits for selection should be considered at different stages,which provided technical parameters for selective breeding for T.ovatus.
为研究不同月龄卵形沙眼选择性组主要形态计量性状对体重的影响,分别测定了1月龄、4月龄、7月龄、10月龄和13月龄2 635只沙眼标本的体长(x1)、体长(x2)、体高(x3)和体重(y)。通过通径分析和多元线性回归计算各性状与体重的相关系数、通径系数和决定系数。分析了各性状对体重的影响,建立了最优回归方程。结果表明:4个性状间的相关系数为0.017 ~ 0.960,除4月龄外,其余4个性状间的相关系数均达到极显著水平(P0.01)。在1月龄和4月龄对体重直接影响最大的性状是体长,其他各阶段对体重直接影响最大的性状是体高。行列式系数分析结果与通径分析结果一致,各阶段方程的拟合度较好(86.9% ~ 90.6%)。结果表明,确定了影响卵圆蝶体重的主要形态计量性状,应在不同阶段进行性状选择,为卵圆蝶的选育提供了技术参数。
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引用次数: 6
Acute toxicity of Cochlodinium geminatum bloom waters from Pearl River Estuary on larvae of brine shrimp,fish and shrimp 珠江口双长氯藻水对咸虾、鱼虾幼虫的急性毒性研究
Q4 Environmental Science Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1231.2013.38620
N. Wu, Tao Jiang, Tian-jiu Jiang, Songhui Lv, Qingliu Huan
Dinoflagellate genus Cochlodinium Schutt has been documented to form blooms along the coastlines of many countries and caused mass mortalities of fish or shellfish.From October to November,2009,harmful algal blooms(HABs)caused by dinoflagellate Cochlodinium geminatum broke out in the coastal waters in Zhuhai,Guangdong.The bloom covers more than 300 km2.In order to understand the toxicity of the bloom water,and provide some basis to the algae bloom management and further study of this species,acute toxicity of the bloom water against larvae of zooplankton Artemia salina,fish Scatophagus argus and shrimp Penaeus vannamei was investigated under room conditions.Moreover,feeding rate of A.salina on C.geminatum was also investigated in this paper.The results showed that LC50(Median Lethal Concentration)of C.geminatum against A.salina larvae was 9.55×104/mL within 24 h.LT50 of C.geminatum against A.salina larvae decreased with the increasing cell density.LT50 of C.geminatum with cell density of 2.5×103/mL against A.salina larvae was 48.5 h.No adverse effects of C.geminatum on the survival of fish and shrimp larvae was observed within 60 h.On the other hand,it was found that both A.salina and S.argus larvae could feed on C.geminatum.But the feeding rate of A.salina larvae was low.Overall,the bloom water of C.geminatum caused an impact on the survival of A.salina.C.geminatum with high cell density could cause mass mortality of A.salina larvae.However,A.salina could live on low cell densities of C.geminatum.Causes of the death of A.salina larvae may not be due to the ingestion of C.geminatum.C.geminatum caused acute toxicity neither against the S.argus larvae nor against the P.vannamei larvae.
据记载,鞭毛藻属cochlordinium Schutt在许多国家的海岸线上形成了大量的水华,造成了鱼类或贝类的大量死亡。2009年10 - 11月,广东珠海市沿海海域爆发了由双氯藻(cochlordinium geminatum)引起的有害藻华。水华覆盖了300多平方公里。为了了解华水的毒性,为华水的管理和藻类的进一步研究提供依据,在室内条件下,研究了华水对浮游动物阿耳蒿(Artemia salina)、泥鳅(Scatophagus argus)和凡纳滨对虾(Penaeus vannamei)幼虫的急性毒性。此外,本文还研究了salina对双胞菌的取食率。结果表明:24 h内,双胞菌对盐盲蝽幼虫的中位致死浓度(LC50)为9.55×104/mL,随着细胞密度的增加,双胞菌对盐盲蝽幼虫的中位致死浓度(lt50)降低;细胞密度为2.5×103/mL的双胞囊藻对双胞囊藻幼虫的LT50为48.5 h, 60 h内未观察到双胞囊藻对鱼虾幼虫的不良影响。另一方面,发现双胞囊藻和argus幼虫均可取食双胞囊藻,但双胞囊藻幼虫的取食率较低。综上所述,高细胞密度的黄花水对双胞沙蝇的生存有一定的影响,可导致双胞沙蝇幼虫大量死亡。salina幼虫的死亡原因可能不是由于误食了c.g inatum, c.g inatum对s.a argus幼虫和p.a annamei幼虫都没有产生急性毒性。
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引用次数: 3
Genetic evaluation and investment appraisal of the multi-trait selection breeding program inLitopenaeus vannamei 凡纳滨对虾多性状选择育种方案的遗传评价与投资评价
Q4 Environmental Science Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1231.2013.38787
Wu Jin, Sheng Luan, J. Kong, Kun Luo, Xianhong Meng, Tianshi Zhang, Qing-yin Wang
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引用次数: 0
The feeding rhythm of veined rapa whelkRapana venosa(Valenciennes) 海螺的摄食节律(瓦朗西纳)
Q4 Environmental Science Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1231.2013.38780
Pingchuan Wang, Libin Zhang, Yang Pan, Tao Zhang, Zhaoxia Li, Shaojun Ban
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引用次数: 0
Population characteristics and distribution ofPseudeuphausia sinicain the Yellow Sea and its relations to the environmental factors 黄海中华伪兔种群特征、分布及其与环境因子的关系
Q4 Environmental Science Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1231.2013.38876
Zhencheng Tao, Chaolun Li, Song Sun
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引用次数: 1
Isolation and identification of ACE inhibitory peptides from the autolysis product of shrimp head(Litopenaeus vannamei) 凡纳滨对虾头自溶产物中ACE抑制肽的分离与鉴定
Q4 Environmental Science Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1231.2013.38204
Guoping Zhu, Chaohua Zhang, Wenhong Cao, Ji Hongwu
Shrimp head is susceptible to autolysis under certain conditions,the protein in it is degraded into soluble protein,peptides and amino acids,and some peptides are active peptides which can inhibit the ACE enzyme activity.At present,many ACE inhibitory peptides derived from food protein have been developed.In the present study,two ACE inhibitory peptides(Tyr-Pro and Leu-Pro/Ile-Pro)were highly purified from the shrimp head(Litopenaeus vannamei)autolysate by extra fine membrane and a series of column chromatographies.In the first autolysis solution of shrimp head was consecutively extracted through extra fine membrane with molecular weight cut-offs(MWCO)at 8,5,3 ku,respectively.The active results shown that filtrate through MWCO at 3 000 u had the highest ACE inhibitory activity.The crude filtrate through MWCO at 3 ku was purified by Sephadex G-25 gel chromatography,SP Sephadex C-25 anion-exchange chromatography as well as Sephadex G-15 gel chromatography,respectively.After that,the ACE inhibitory activity of purified filtrate almost increased by 8 times(IC50=0.19 mg/mL)that of crude filtrate.The high active collected fraction from Sephadex G-15 gel chromatography was carried out by RP-HPLC(High-Performance Liquid Chromatography)twice for the further purification and two kinds of dipeptide were obtained,and the identification of dipeptide by mass spectra showed that they were Tyr-Pro and Leu-Pro/Ile-Pro,and the molecular weight was 279 u and 229 u,respectively.
虾头在一定条件下易发生自溶,蛋白质降解为可溶性蛋白、多肽和氨基酸,部分多肽为抑制ACE酶活性的活性多肽。目前已开发出许多从食品蛋白中提取的ACE抑制肽。本研究采用超细膜和一系列柱层析技术,从凡纳滨对虾头自溶物中高度纯化了两种ACE抑制肽(Tyr-Pro和Leu-Pro/Ile-Pro)。在第一次自溶液中,虾头分别用分子量临界值为8、5、3 ku的超细膜连续提取。活性结果表明,3 000 u MWCO滤液的ACE抑制活性最高。采用Sephadex G-25凝胶层析、SP Sephadex C-25阴离子交换层析和Sephadex G-15凝胶层析对3ku MWCO粗滤液进行纯化。之后,纯化滤液的ACE抑制活性几乎比粗滤液提高了8倍(IC50=0.19 mg/mL)。对从Sephadex G-15凝胶层析中收集的高活性组分进行两次高效液相色谱(RP-HPLC)进一步纯化,得到两种二肽,质谱鉴定为Tyr-Pro和Leu-Pro/Ile-Pro,分子量分别为279 u和229 u。
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引用次数: 0
Molecular cloning and prokaryotic expression of cyclin B from shrimp Metapenaeus affinis 拟对虾细胞周期蛋白B的分子克隆及原核表达
Q4 Environmental Science Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1231.2013.38210
Yanan Yang, Huiyang Huang, Xiaoshuai Huang, Haihui Ye, Li Shaojing
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引用次数: 2
Effects on survival time of Fenneropenaeus chinensis challenged by different doses of WSSV and diets 不同剂量WSSV和饲料攻毒对中国对虾存活时间的影响
Q4 Environmental Science Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1231.2013.38261
Li Xupeng, Meng Xianhong, Kong Jie, Cao Baoxiang, L. Ning, Luo Kun, Liu Weiya
White spot syndrome virus(WSSV)is one kind of destructive virus affecting shrimp industry in the world.Output of Fenneropenaeus chinensis which once was a staple species has been greatly affected by outbreak of WSSV since 1993 in China.To produce WSSV-resistance shrimp strains is an important approach to revive the shrimp industry.To select survived F.chinensis as broodstock via WSSV challenge test can increase the efficiency of breeding.However,mortality of shrimps could reach nearly 100% once they were infected with WSSV,which increases the difficulty for shrimp breeding.In this study for the purpose to reveal effect of different WSSV amount on F.chinensis,juvenile F.chinensis were infected with a certain amount of WSSV individually and the survival time and survival rate were observed.The results showed that survival time of shrimps infected with 5.2×108 copies,1.0×109 copies and 2.1×109 copies of WSSV respectively was 389.3,323.3 and 187.3 h and significant different(P0.01).The cumulative mortalities of all the shrimps finally reached 100%.The survival time increased while shrimps were fed with less WSSV bait.For the purpose to reveal the effect of diets on the ability for disease resistance,shrimps infected with WSSV were fed with different diets of live brine shrimps,commercial bait and fresh clam.It was showed that the survival time of infected shrimps fed with live brine shrimps,fresh clam and commercial bait respectively was 281.7,173.9 and 164.9 h.The survival time of infected shrimp fed with live brine shrimps was significantly higher than that fed by commercial bait and fresh clam respectively(P0.01).There was no significant difference of survival time between infected shrimps fed with commercial bait and fresh clam(P0.05).Cumulative mortalities of all the groups of infected shrimps that fed with different diets were 100%.Live brine shrimp is better than commercial bait and fresh clam for the purpose of enhancing shrimp WSSV resistance ability.
白斑综合征病毒(White spot syndrome virus, WSSV)是世界上影响对虾产业的一种破坏性病毒。自1993年WSSV爆发以来,曾是中国主要养殖品种的中国对虾产量受到严重影响。培育抗wssv对虾品系是振兴对虾产业的重要途径。通过WSSV攻毒试验选择存活的中华黄芪作为亲本,可以提高育种效率。然而,一旦感染WSSV,虾的死亡率可达到近100%,这增加了虾的养殖难度。本研究为揭示不同WSSV量对中华绒螯蟹的影响,分别用一定数量的WSSV侵染中华绒螯蟹幼体,观察其存活时间和存活率。结果表明,感染WSSV 5.2×108拷贝、1.0×109拷贝和2.1×109拷贝的对虾的存活时间分别为389.3,323.3和187.3 h,差异有统计学意义(P0.01)。所有虾的累积死亡率最终达到100%。饵料越少,虾的存活时间越长。为研究不同饵料对WSSV侵染对虾抗病能力的影响,分别饲喂活卤虾、商品饵料和鲜蛤蜊饵料。结果表明,分别饲喂活卤虾、鲜蛤蜊和商品饵料的病虾存活时间分别为281.17、173.9和164.9 h,分别饲喂活卤虾和鲜蛤蜊的病虾存活时间显著高于商品饵料和鲜蛤蜊(P0.01)。饵料饵料与鲜蛤蜊饵料的成活率无显著差异(P0.05)。饲喂不同饵料的各组受感染虾的累积死亡率均为100%。在提高对虾抗WSSV能力方面,活盐水虾优于商品饵料和鲜蛤蜊。
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引用次数: 0
Research on algicidal effect of bioactive metabolites of Brevibacillus laterosporus on Oscillatoria sp.in shrimp pond 后芽孢短杆菌生物活性代谢物对虾池中振荡藻的杀藻效果研究
Q4 Environmental Science Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1231.2013.38269
W. Jia, Xianghu Huang, Changling Li, Jia-hui Zhang
Oscillatoria sp.is a common and harmful cyanophyte in shrimp culture pond.In this study,the sterile filtrate of Brevibacillus laterosporus and Oscillatoria sp.were used to study the algicidal effects in their different growth stages.The algae dry weight,chlorophyll-a and phycocyanin content were measured in order to explore the best algae-lysing period and the algicidal mechanism.The final aim was to provide the basic theory for improving water environment in shrimp pond.The results showed that algicidal effects of the sterile filtrate on Oscillatoria sp.were very significant(P0.01)when the algae-lysing bacteria was in the stable and decline phases.After 7 days,the algae dry weights were reduced by 51.77%,47.04% respectively,the contents of chlorophyll-a were decreased by 67.60%,59.13% respectively;the phycocyanin contents were increased by 33.51%,30.97%,respectively.Similarly,the algicidal effect of the sterile filtrate of algae-lysing bacteria was so conspicuous(P0.01)on the lag phase Oscillatoria sp..7 days later,the algae dry weight was reduced by 63.90%;the content of chlorophyll-a was decreased by 69.72%;the phycocyanin content was increased by 54.17%.The conclusion was that the sterile filtrate of Brevibacillus laterosporus in the stable phase had the best algicidal effect on the lag phase Oscillatoria sp..
振荡藻是对虾养殖池中常见的有害蓝藻。本研究以短芽孢杆菌(Brevibacillus laterosporus)和振荡芽孢杆菌(Oscillatoria sp.)的无菌滤液为材料,研究了它们在不同生长阶段的杀藻效果。通过测定藻类干重、叶绿素-a和藻蓝蛋白含量,探索最佳的溶藻期和杀藻机理。最终目的是为改善虾塘水环境提供理论依据。结果表明,当溶藻菌处于稳定期和衰退期时,无菌滤液对振荡菌的杀藻效果极显著(P0.01)。处理7 d后,藻类干重分别降低了51.77%、47.04%,叶绿素a含量分别降低了67.60%、59.13%,藻蓝蛋白含量分别提高了33.51%、30.97%。同样,溶藻菌无菌滤液对滞相振荡菌的杀藻效果也非常显著(P0.01)。7 d后,藻类干重降低63.90%,叶绿素-a含量降低69.72%,藻蓝蛋白含量提高54.17%。结果表明,稳定期后芽孢短杆菌无菌滤液对滞后期振荡菌的杀藻效果最好。
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引用次数: 2
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