Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38817
L. Yongxin, Z. Yi-mei, Liu Yingjie, Gao Lei, Fang Hui, H. Gang, Wang Yufen, Ji Xiufeng, Liu Haijin
To analyze the genetic diversity of natural population and select the specific microsatellite markers for identifying effectively genetic characteristics of population in Japanese flounder( Paralichthys olivaceus)in this study,seventy-four w ild individuals captured from the Bohai Sea w ere used to form the experimental population. A set of 72 microsatellite markers located on different regions in linkage group w ere chosen to carry out the genetic analysis. In these markers,17 w ere located in the centromeric region,36 in the distant region from the centromere and 19 in the intermediate region of linkage groups. Analysis show ed that the number of alleles( A) ranged from 6. 400 to 7. 389,number of effective alleles( N e) ranged from 4. 469 to5. 129 and Shannon's information index( I) ranged from 1. 565 to 1. 683 in different regions of linkage group. The observed heterozygosity( H o),unbiased expected heterozygosity( H e) and polymorphism information content( PIC) w as from 0. 568 to 0. 593,from 0. 738 to 0. 753 and from 0. 707 to 0. 746,respectively. The Hardy-Weinberg departure value( d) w as low er than- 0. 200,w hile,genetic distance( G D)among individuals in population w as above than 0. 620. Each genetic parameter demonstrated that there w as richer genetic diversity in experimental population and relatively farther genetic distance among individuals.This population w as suitable for conducting selective breeding as founder population. Significant difference did not exist in the genetic parameters obtained by microsatellite markers located on different regions in linkage group,w hich verified that there w as no necessary correlation betw een the positions of marker located and the degree of genetic diversity of markers. How ever,these polymorphic markers could be used as marker candidates to analyze the genetic structure in population of Japanese flounder.
为了分析日本比目鱼自然种群的遗传多样性，选择具有特异性的微卫星标记，有效鉴定日本比目鱼种群的遗传特征，本研究以捕获的74条渤海野生个体为研究对象，组成实验种群。选择位于连锁群不同区域的72个微卫星标记进行遗传分析。在这些标记中，有17个位于着丝粒区，36个位于着丝粒远区，19个位于连锁群的中间区。分析结果表明，等位基因数量(A)为6个。400比7。389，有效等位基因数(N e)为4。469卡车。Shannon’s information index(I)的取值范围为1。565比1。683在不同区域的连锁组。观察杂合度(H o)、无偏期望杂合度(H e)和多态性信息含量(PIC) w从0开始。568比0。593年,从0。738到0。753和0。707比0。746年,分别。Hardy-Weinberg偏离值(d) w小于- 0。种群中个体间遗传距离(gd)大于0。620. 各遗传参数均表明实验群体具有较丰富的遗传多样性，个体间遗传距离相对较远。该群体与创始群体一样适合进行选择性育种。位于连锁群不同区域的微卫星标记获得的遗传参数没有显著差异，验证了标记所处位置与标记的遗传多样性程度之间没有必然的相关性。这些多态标记可以作为候选标记，用于分析牙鲆群体的遗传结构。
{"title":"Analysis of genetic diversity in Bohai natural population of Paralichthys olivaceus.","authors":"L. Yongxin, Z. Yi-mei, Liu Yingjie, Gao Lei, Fang Hui, H. Gang, Wang Yufen, Ji Xiufeng, Liu Haijin","doi":"10.3724/SP.J.1231.2013.38817","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38817","url":null,"abstract":"To analyze the genetic diversity of natural population and select the specific microsatellite markers for identifying effectively genetic characteristics of population in Japanese flounder( Paralichthys olivaceus)in this study,seventy-four w ild individuals captured from the Bohai Sea w ere used to form the experimental population. A set of 72 microsatellite markers located on different regions in linkage group w ere chosen to carry out the genetic analysis. In these markers,17 w ere located in the centromeric region,36 in the distant region from the centromere and 19 in the intermediate region of linkage groups. Analysis show ed that the number of alleles( A) ranged from 6. 400 to 7. 389,number of effective alleles( N e) ranged from 4. 469 to5. 129 and Shannon's information index( I) ranged from 1. 565 to 1. 683 in different regions of linkage group. The observed heterozygosity( H o),unbiased expected heterozygosity( H e) and polymorphism information content( PIC) w as from 0. 568 to 0. 593,from 0. 738 to 0. 753 and from 0. 707 to 0. 746,respectively. The Hardy-Weinberg departure value( d) w as low er than- 0. 200,w hile,genetic distance( G D)among individuals in population w as above than 0. 620. Each genetic parameter demonstrated that there w as richer genetic diversity in experimental population and relatively farther genetic distance among individuals.This population w as suitable for conducting selective breeding as founder population. Significant difference did not exist in the genetic parameters obtained by microsatellite markers located on different regions in linkage group,w hich verified that there w as no necessary correlation betw een the positions of marker located and the degree of genetic diversity of markers. How ever,these polymorphic markers could be used as marker candidates to analyze the genetic structure in population of Japanese flounder.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"37 1","pages":"1609-1617"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69971160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.27834
Lili Yang, G. He, Zhong-jun Hu, Wen-Jing Shi, Qigen Liu, Laisheng Chen, Jinpeng Wang, Weiming Yang
{"title":"Community structure of zooplankton and its relation to environmental factors in the silver-carp-and-bighead-carp-dominated Qiandao Lake","authors":"Lili Yang, G. He, Zhong-jun Hu, Wen-Jing Shi, Qigen Liu, Laisheng Chen, Jinpeng Wang, Weiming Yang","doi":"10.3724/SP.J.1231.2013.27834","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.27834","url":null,"abstract":"","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"37 1","pages":"894"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69962350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38099
L. Zhiyuan, Li Jianrong, Li Xuepeng, Li Tingting, Wang Yanbo, Huang He, Chen Huajian, Lu Weike
In order to use two-dimensional electrophoresis(2-DE)analysis in the muscle proteins of Chinese shrimp(Fenneropenaeus chinensis),its muscle proteins were separated using immobilized pH gradient 2-DE after dissolving.By optimizing different extraction methods,pH of IPG gel strips and its length,isoelectric focusing programs,and loading amount,etc,the proteins were successfully extracted from Chinese shrimp muscle and were separated by 2-DE.After silver staining or Coomassie brilliant blue staining,PDQuest image analysis software was applied to analyze the 2-DE images.The results showed that most of shrimp muscle protein isoelectric points were between 4 and 7.The 2-DE related techniques was constructed and optimized in muscle proteome of Chinese shrimp by comparative tests on different extraction methods,IPG gel strips,isoelectric focusing programs,salt bridge,and sample volume,etc.The results showed that the resolution and reproducibility of 2-DE profiles were significantly improved by adding thiourea,CHAPS and DTT in lysis buffer,active rehydrating of 17 cm(pH 4-7)IPG gel strips,loading the sample 120 μg,prolonging the time of desalting,increasing the voltage and power of isoelectric focusing,employing the salt bridge,preparing 12.5% SDS-PAGE gel,and dying the gels by the silver staining.It shows that the repeatability and stability are good enough.
{"title":"Establishment of two-dimensional electrophoresis (2-DE) technique in muscle proteome of Fenneropenaeus chinensis.","authors":"L. Zhiyuan, Li Jianrong, Li Xuepeng, Li Tingting, Wang Yanbo, Huang He, Chen Huajian, Lu Weike","doi":"10.3724/SP.J.1231.2013.38099","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38099","url":null,"abstract":"In order to use two-dimensional electrophoresis(2-DE)analysis in the muscle proteins of Chinese shrimp(Fenneropenaeus chinensis),its muscle proteins were separated using immobilized pH gradient 2-DE after dissolving.By optimizing different extraction methods,pH of IPG gel strips and its length,isoelectric focusing programs,and loading amount,etc,the proteins were successfully extracted from Chinese shrimp muscle and were separated by 2-DE.After silver staining or Coomassie brilliant blue staining,PDQuest image analysis software was applied to analyze the 2-DE images.The results showed that most of shrimp muscle protein isoelectric points were between 4 and 7.The 2-DE related techniques was constructed and optimized in muscle proteome of Chinese shrimp by comparative tests on different extraction methods,IPG gel strips,isoelectric focusing programs,salt bridge,and sample volume,etc.The results showed that the resolution and reproducibility of 2-DE profiles were significantly improved by adding thiourea,CHAPS and DTT in lysis buffer,active rehydrating of 17 cm(pH 4-7)IPG gel strips,loading the sample 120 μg,prolonging the time of desalting,increasing the voltage and power of isoelectric focusing,employing the salt bridge,preparing 12.5% SDS-PAGE gel,and dying the gels by the silver staining.It shows that the repeatability and stability are good enough.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"37 1","pages":"288-296"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69963093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38105
Jiaia Zheng, Lisha Peng, Xiao-ping Zhang, Jiandong Sun, L. Fu, Yibing Wang, Caiye Hu, Li Weifen
This experiment was conducted to study the effects of complex probiotics on water quality and microbial community structure in grass carp culture.The results of water quality determination showed that compared with the control group,the concentration of ammonia nitrogen,nitrite nitrogen and total nitrogen in treatment group was lower in the whole experiment,respectively;On 18th d,the concentration of nitrate nitrogen in treatment group was decreased by 54.49%(P0.05).On 15th d,the concentration of total inorganic nitrogen in treatment group was decreased by 28.75%(P0.05).Orthophosphate and total phosphorus had no difference between the two groups.The 454-Pyrosequencing results showed that the composition of bacterial communities was highly variable between control and treatment groups,and microbial diversity of treatment was higher than that of control.Firmicutes and Proteobacteria decreased by 91.21%(P0.01)and 21.75%(P0.01)respectively.Bacteroidetes,Actinobacteria and Cyanobacteria increased by 288%(P0.01),435%(P0.01)and 848%(P0.01)respectively.Among Proteobacteria,Alphaproteobacteria and Betaproteobacteria was elevated by 318%(P0.01)and 18% respectively,while Gammaproteobacteria was reduced by 78.82%(P0.01).
{"title":"Effects of complex probiotics on water quality and microbial communities in grass carp(Ctenopharyngodon idellus)culture","authors":"Jiaia Zheng, Lisha Peng, Xiao-ping Zhang, Jiandong Sun, L. Fu, Yibing Wang, Caiye Hu, Li Weifen","doi":"10.3724/SP.J.1231.2013.38105","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38105","url":null,"abstract":"This experiment was conducted to study the effects of complex probiotics on water quality and microbial community structure in grass carp culture.The results of water quality determination showed that compared with the control group,the concentration of ammonia nitrogen,nitrite nitrogen and total nitrogen in treatment group was lower in the whole experiment,respectively;On 18th d,the concentration of nitrate nitrogen in treatment group was decreased by 54.49%(P0.05).On 15th d,the concentration of total inorganic nitrogen in treatment group was decreased by 28.75%(P0.05).Orthophosphate and total phosphorus had no difference between the two groups.The 454-Pyrosequencing results showed that the composition of bacterial communities was highly variable between control and treatment groups,and microbial diversity of treatment was higher than that of control.Firmicutes and Proteobacteria decreased by 91.21%(P0.01)and 21.75%(P0.01)respectively.Bacteroidetes,Actinobacteria and Cyanobacteria increased by 288%(P0.01),435%(P0.01)and 848%(P0.01)respectively.Among Proteobacteria,Alphaproteobacteria and Betaproteobacteria was elevated by 318%(P0.01)and 18% respectively,while Gammaproteobacteria was reduced by 78.82%(P0.01).","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"44 1","pages":"457"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69963155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38236
Haiying Liang, Yongxin Cheng, J. Jian, Zao-he Wu
In order to study the immunogenic and protective effects of DNA vaccine,plasmid DNA encoding flagellin flaC gene(designated as pcDNA-flaC)was used as a DNA vaccine to immunize red snapper(Lutjanus sanguineus).The distribution,expression and immunoprotection of the DNA vaccine were analyzed in tissues of the red snapper by PCR,RT-PCR and challenge test.PCR results indicated that pcDNA-flaC was distributed in liver,spleen,kidney,gill and injection site muscle at 7-28 days after vaccination.RT-PCR results indicated that the flaC gene was expressed in all above tissues of vaccinated fish at 7-28 days after vaccination.These results demonstrated that the DNA vaccine was distributed and flaC gene was expressed in various tissues of vaccinated fish.Red snapper immunized with DNA vaccine showed higher serum antibody levels at 7-28 days after vaccination,compared to fish vaccinated with the control eukaryotic expression vector pcDNA3.1 and PBS.In addition,fish immunized with DNA vaccine developed a protective response to live V.alginolyticus challenge 28 days post-inoculation,as demonstrated by increased survival of vaccinated fish over the control fish.This study indicates that pcDNA-flaC is an effective vaccine candidate against V.alginolyticus infection.
{"title":"Immunogenic and protective effects of a DNA vaccine containing flagellin flaC gene against Vibrio alginolyticus in red snapper(Lutjanus sanguineus)","authors":"Haiying Liang, Yongxin Cheng, J. Jian, Zao-he Wu","doi":"10.3724/SP.J.1231.2013.38236","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38236","url":null,"abstract":"In order to study the immunogenic and protective effects of DNA vaccine,plasmid DNA encoding flagellin flaC gene(designated as pcDNA-flaC)was used as a DNA vaccine to immunize red snapper(Lutjanus sanguineus).The distribution,expression and immunoprotection of the DNA vaccine were analyzed in tissues of the red snapper by PCR,RT-PCR and challenge test.PCR results indicated that pcDNA-flaC was distributed in liver,spleen,kidney,gill and injection site muscle at 7-28 days after vaccination.RT-PCR results indicated that the flaC gene was expressed in all above tissues of vaccinated fish at 7-28 days after vaccination.These results demonstrated that the DNA vaccine was distributed and flaC gene was expressed in various tissues of vaccinated fish.Red snapper immunized with DNA vaccine showed higher serum antibody levels at 7-28 days after vaccination,compared to fish vaccinated with the control eukaryotic expression vector pcDNA3.1 and PBS.In addition,fish immunized with DNA vaccine developed a protective response to live V.alginolyticus challenge 28 days post-inoculation,as demonstrated by increased survival of vaccinated fish over the control fish.This study indicates that pcDNA-flaC is an effective vaccine candidate against V.alginolyticus infection.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"1 1","pages":"125"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69963904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38253
Y. Zheng, W. Hong, Qiyong Zhang
Historical investigation and the achievements of resources biology of main marine demersal food fishes along the coastal waters of China,with special regard to the ribbon fish(Trichiurus japonicus),small yellow croaker(Larimichthys polyactis),large yellow croaker(Larimichthys crocea)and black scraper(Thamnaconus modestus),are reviewed in this paper.This review covers the identification and classification of fish geographical populations and their spawning stocks,historical pattern changes,the structure of populations and stocks,age composition and growth characteristics,feeding habits,sex mature cycles,gonadosomatic index(GSI),fecundity of the spawning stocks,spawning grounds,feeding grounds,wintering grounds,migration routes,assessment of amounts of nature resources and catches,adjustment of fishing boats,and restricted measures for fishing effort.The prospects and problems of resource biology of main marine demersal food fishes along the coastal waters of China are also discussed.
{"title":"Review and prospects for resource biology of main marine demersal food fishes along the coastal waters of China","authors":"Y. Zheng, W. Hong, Qiyong Zhang","doi":"10.3724/SP.J.1231.2013.38253","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38253","url":null,"abstract":"Historical investigation and the achievements of resources biology of main marine demersal food fishes along the coastal waters of China,with special regard to the ribbon fish(Trichiurus japonicus),small yellow croaker(Larimichthys polyactis),large yellow croaker(Larimichthys crocea)and black scraper(Thamnaconus modestus),are reviewed in this paper.This review covers the identification and classification of fish geographical populations and their spawning stocks,historical pattern changes,the structure of populations and stocks,age composition and growth characteristics,feeding habits,sex mature cycles,gonadosomatic index(GSI),fecundity of the spawning stocks,spawning grounds,feeding grounds,wintering grounds,migration routes,assessment of amounts of nature resources and catches,adjustment of fishing boats,and restricted measures for fishing effort.The prospects and problems of resource biology of main marine demersal food fishes along the coastal waters of China are also discussed.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"37 1","pages":"151"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69964262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38282
Hai Lin, Gang Zhou, Xuguang Li, Jun Zhou, Tongqing Zhang, Gui-Ping Wang
In order to analyze the seasonal variation characteristics of emissions of CO2,CH4,N2O and evaluate the comprehensive global warming potentials(GWPs),fluxes of CO2,CH4 and N2O were measured simultaneously from the culture ponds of Chinese mitten crabs(Eriocheir sinensis)by the closed static opaque chamber-GC techniques.The results showed that the pond ecosystem was the sources of CO2 and CH4 in summer.The total emission of CO2 and CH4 came up to(42.62±9.55)-(95.09±10.89)g/m2 (6.91±2.18)-(12.52±3.11)g/m2 in summer.There was significantly lower emission of CO2/CH4 from the water with aquatic plants than the one without.Compared with the waters without aquatic plants,the aquatic plants can effectively decrease and control the emissions by 52.47 g/m2 of CO2 and 5.61 g/m2 of CH4.They are proved to reduce greenhouse gas emission significantly from culture ponds,but no significant effect on the emission of N2O with aquatic plants.It may decrease global warming potential by 1.85 times contrasted to that without aquatic plants.There may be great potential space of reducing greenhouse gases emission in pond culture of Chinese mitten crabs.
{"title":"Greenhouse gases emissions from pond culture ecosystem of Chinese mitten crab and their comprehensive global warming potentials in summer","authors":"Hai Lin, Gang Zhou, Xuguang Li, Jun Zhou, Tongqing Zhang, Gui-Ping Wang","doi":"10.3724/SP.J.1231.2013.38282","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38282","url":null,"abstract":"In order to analyze the seasonal variation characteristics of emissions of CO2,CH4,N2O and evaluate the comprehensive global warming potentials(GWPs),fluxes of CO2,CH4 and N2O were measured simultaneously from the culture ponds of Chinese mitten crabs(Eriocheir sinensis)by the closed static opaque chamber-GC techniques.The results showed that the pond ecosystem was the sources of CO2 and CH4 in summer.The total emission of CO2 and CH4 came up to(42.62±9.55)-(95.09±10.89)g/m2 (6.91±2.18)-(12.52±3.11)g/m2 in summer.There was significantly lower emission of CO2/CH4 from the water with aquatic plants than the one without.Compared with the waters without aquatic plants,the aquatic plants can effectively decrease and control the emissions by 52.47 g/m2 of CO2 and 5.61 g/m2 of CH4.They are proved to reduce greenhouse gas emission significantly from culture ponds,but no significant effect on the emission of N2O with aquatic plants.It may decrease global warming potential by 1.85 times contrasted to that without aquatic plants.There may be great potential space of reducing greenhouse gases emission in pond culture of Chinese mitten crabs.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"37 1","pages":"417"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69964571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38337
Yuling Yang, Wei Li, Weizhou Chen, Juntian Xu
{"title":"Effects of CO2and UV radiation on photosynthetic performance of conchocelis ofPyropia haitanensis","authors":"Yuling Yang, Wei Li, Weizhou Chen, Juntian Xu","doi":"10.3724/SP.J.1231.2013.38337","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38337","url":null,"abstract":"","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"37 1","pages":"1198"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69965343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38343
Jianjian Lü, Yu Wang, B. Gao, Jian Li, Ping Liu
In the present study,typeⅠmicrosatellites of Portunus trituberculatus were screened from 13 985 ESTs in the NCBI database by bioinformatics approach.A total of 287 type Ⅰ microsatellites were identified,among which 173 were the type of dinucleotide repeat being the most abundant microsatellite and accounting for 60.3% of the identified microsatellite markers,and 79 were the type of trinucleotide accounting for 27.5%,while hexa-,penta-and tetra-nucleotide repeats were rare.Among the dinucleotide repeats,AC/GT was the most abundant type with percentage of 53.8%,followed by the AG/CT type with 37.0%,while AT and GC were rare.14 microsatellites were selected for polymorphism analyses,8 of them showed polymorphism in a wild population of P.trituberculatus.The mean PIC and heterozygosity values were 0.57 and 0.63,respectively.Among them,6 PIC of microsatellites greater than 0.5,showing high polymorphism.This study showed that the way of screening typeⅠmicrosatellite markers from EST data was feasible.These typeⅠmicrosatellites can be used for further study in genetic diversity,genetic mapping and quantitative trait loci(QTLs)identification in P.trituberculatus.
{"title":"Identification of type I microsatellite markers and their polymorphism in Portunus trituberculatus","authors":"Jianjian Lü, Yu Wang, B. Gao, Jian Li, Ping Liu","doi":"10.3724/SP.J.1231.2013.38343","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38343","url":null,"abstract":"In the present study,typeⅠmicrosatellites of Portunus trituberculatus were screened from 13 985 ESTs in the NCBI database by bioinformatics approach.A total of 287 type Ⅰ microsatellites were identified,among which 173 were the type of dinucleotide repeat being the most abundant microsatellite and accounting for 60.3% of the identified microsatellite markers,and 79 were the type of trinucleotide accounting for 27.5%,while hexa-,penta-and tetra-nucleotide repeats were rare.Among the dinucleotide repeats,AC/GT was the most abundant type with percentage of 53.8%,followed by the AG/CT type with 37.0%,while AT and GC were rare.14 microsatellites were selected for polymorphism analyses,8 of them showed polymorphism in a wild population of P.trituberculatus.The mean PIC and heterozygosity values were 0.57 and 0.63,respectively.Among them,6 PIC of microsatellites greater than 0.5,showing high polymorphism.This study showed that the way of screening typeⅠmicrosatellite markers from EST data was feasible.These typeⅠmicrosatellites can be used for further study in genetic diversity,genetic mapping and quantitative trait loci(QTLs)identification in P.trituberculatus.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":"37 1","pages":"816"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69965410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38389
Lifang Feng, Xing Cheng, Shanshan He, Jian-rong Li
Vibrio vulnificus is a marine seafood-borne pathogen that will cause death in susceptible individuals after consumption of raw or uncooked contaminated seafood around the world.So,early detection and identification of V.vulnificus strains in food and clinical samples is essential for diagnosis and reducing the incidence of food-borne disease.PCR assay has been one of the most important and extensive method to detect pathogenic bacteria.Previous study depended on vvhA as the target gene to detect this bacterium.In this study,we constructed a local BLAST database and identified 34 candidates as V.vulnificus-specific target genes distributed in 3 isolates(CMCP6,MO6-24/O,and YJ016)with published completed genome.Among these candidate-specific targets,VV1_2692,VV2_0075,and VV2_0939 genes are known for their functions,while the rests encode hypothetical protein of unknown function.To evaluate the specificity of above 3 genes,PCR amplification of genomic DNA from a V.vulnificus strain resulted in a product with predicted length,whereas no products were detected from 14 non-V.vulnificus bacterial strains.The minimum detectable limits of VV1_2692,VV2_0075,and VV2_0939 genes were 103,101,and 102 cfu/mL,respectively.A total of 137 seafood samples(e.g.,fish,prawn,crab,shell)from Hangzhou city were detected by both PCR assay and biochemical method.Among them,39 V.vulnificus isolates were detected using biochemical method,while 38,39,39,and 39 V.vulnificus isolates were detected using PCR assay of VV1_2692,VV2_0075,VV2_0939,and vvhA genes,respectively.The negative result of VV1_2692 probably resulted from its relative high detectable limit,and both VV2_0075 and VV2_0939 genes might be suitable species-specific targets to detect V.vulnificus in seafood.Moreover,28.5% of 137 seafood samples contained V.vulnificus,and oyster had the highest ratio(68%),suggesting status of V.vulnificus pollution was extremely serious in coastal seafood of Hangzhou city,especially oyster.
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