Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38506
Liping Li, Rui Wang, Ting Huang, Weiyi Huang, W. Liang, Jian Li, Y. Huang, A. Lei, X. Gan, Ming Chen
To acquire the data upon the clinical dominant strains of tilapia Streptococcus spp.and their genotype variations in Guangxi province,a total of 77 clinical Streptococcus spp.strains collected from the epidemic areas of Guangxi from 2006 to 2012 were identified by species-specific PCR.Among them,37 strains collected from 2006 to 2011 were genotyped by the pulse field gel electrophoresis(PFGE)method.As a result,57 out of 77 strains were identified as Streptococcus agalactiae,and the rest were determined as S.iniae by PCR identification process.As is shown,the dominant strains from 2006 to 2007 were S.iniae,accounted for 94.7%(18/19),only 5.3%(1/19)was S.agalactiae strains.Between 2009 and 2012,the dominant strains,however,became S.agalactiae,which accounted for 96.6%(56/58),only 3.4%(2/58)was referred as S.iniae strains.The strains were divided into two branches by PFGE genotyping.20 S.iniae strains were in one classification with 4 PFGE genotypes,the similarities of which were 83.9%-100%.17 S.agalactiae strains were put in another branch with 5 PFGE genotypes,the similarities of which were between 47.4% and 100%.Our data confirmed that the prevalent strains of tilapia Streptococcus spp.in Guangxi province have been shifting from S.iniae(before 2008)to S.agalactiae(2009-2012),and the PFGE genetic diversities were demonstrated in the prevalent strains.
{"title":"PCR detection and PFGE genotype analyses of streptococcal clinical isolates from tilapia in Guangxi","authors":"Liping Li, Rui Wang, Ting Huang, Weiyi Huang, W. Liang, Jian Li, Y. Huang, A. Lei, X. Gan, Ming Chen","doi":"10.3724/SP.J.1231.2013.38506","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38506","url":null,"abstract":"To acquire the data upon the clinical dominant strains of tilapia Streptococcus spp.and their genotype variations in Guangxi province,a total of 77 clinical Streptococcus spp.strains collected from the epidemic areas of Guangxi from 2006 to 2012 were identified by species-specific PCR.Among them,37 strains collected from 2006 to 2011 were genotyped by the pulse field gel electrophoresis(PFGE)method.As a result,57 out of 77 strains were identified as Streptococcus agalactiae,and the rest were determined as S.iniae by PCR identification process.As is shown,the dominant strains from 2006 to 2007 were S.iniae,accounted for 94.7%(18/19),only 5.3%(1/19)was S.agalactiae strains.Between 2009 and 2012,the dominant strains,however,became S.agalactiae,which accounted for 96.6%(56/58),only 3.4%(2/58)was referred as S.iniae strains.The strains were divided into two branches by PFGE genotyping.20 S.iniae strains were in one classification with 4 PFGE genotypes,the similarities of which were 83.9%-100%.17 S.agalactiae strains were put in another branch with 5 PFGE genotypes,the similarities of which were between 47.4% and 100%.Our data confirmed that the prevalent strains of tilapia Streptococcus spp.in Guangxi province have been shifting from S.iniae(before 2008)to S.agalactiae(2009-2012),and the PFGE genetic diversities were demonstrated in the prevalent strains.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"927"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69968180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38695
D. Zhang
{"title":"Applications of aquatic animal behavior in aquaculture","authors":"D. Zhang","doi":"10.3724/SP.J.1231.2013.38695","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38695","url":null,"abstract":"","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"1591"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69970280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Retinoid X receptor( RXR) is a member of the second subfamily of nuclear receptor superfamily. In the insect and the crustacean,RXR is an important signaling protein w ith varied roles in regulating aspects of reproductive maturation,molting and embryo development. In this study,w e cloned RXR gene from Eriocheir sinensis using reverse transcriptase polymerase chain reaction( RT-PCR) and rapid-amplification of cDNA ends( RACE),and primers w ere designed according to the conserved sequence of RXR from G ecarcinus lateralis. The full-length cDNA sequence of RXR is 1 517 bp and codes a protein of about 433 amino acids. The amino acid sequence comparison results show ed that the RXR gene of Eriocheir sinensis shared 96% identity w ith G ecarcinus lateralis,using BLASTn and BLASTx softw are. Phylogenetic tree of RXR gene generated by Neighbor Joining method suggested that RXR is clustered closely w ith that of G ecarcinus lateralis. The expression of the gene in different tissues and molting stages of E. sinensis w as analyzed by real-time fluorescent quantitative PCR. The result show ed the RXR mRNA w as expressed in all tissues examined and highly in Y-organ,w ith small amount in hepatopancreas,gill and muscle,trace in heart, stomach,intestine,thoracic ganglion and brain ganglion. RXR mRNA w as detected w ith high volume in Yorgan compared to hepatopancreas,muscles and gill at the same molting stages of the crab. RXR mRNA of Y-organ in stage D w as significantly( P 0. 05) higher than that of stage AB and stage C. It also had significant difference( P 0. 05) betw een muscles at stage AB and stage E,and there w as no significant difference expression in hepatopancreas and gill tissues in different molting stages.
{"title":"The full length cDNA cloning and expression analysis of RXR from the Chinese mitten crab(Eriocheir sinensis)","authors":"Yao Wang, Zhigang Yang, Zi-hao Guo, Qinqin Yao, Qitao Zeng, Xiaozhen Yang, Yongxu Cheng","doi":"10.3724/SP.J.1231.2013.38754","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38754","url":null,"abstract":"Retinoid X receptor( RXR) is a member of the second subfamily of nuclear receptor superfamily. In the insect and the crustacean,RXR is an important signaling protein w ith varied roles in regulating aspects of reproductive maturation,molting and embryo development. In this study,w e cloned RXR gene from Eriocheir sinensis using reverse transcriptase polymerase chain reaction( RT-PCR) and rapid-amplification of cDNA ends( RACE),and primers w ere designed according to the conserved sequence of RXR from G ecarcinus lateralis. The full-length cDNA sequence of RXR is 1 517 bp and codes a protein of about 433 amino acids. The amino acid sequence comparison results show ed that the RXR gene of Eriocheir sinensis shared 96% identity w ith G ecarcinus lateralis,using BLASTn and BLASTx softw are. Phylogenetic tree of RXR gene generated by Neighbor Joining method suggested that RXR is clustered closely w ith that of G ecarcinus lateralis. The expression of the gene in different tissues and molting stages of E. sinensis w as analyzed by real-time fluorescent quantitative PCR. The result show ed the RXR mRNA w as expressed in all tissues examined and highly in Y-organ,w ith small amount in hepatopancreas,gill and muscle,trace in heart, stomach,intestine,thoracic ganglion and brain ganglion. RXR mRNA w as detected w ith high volume in Yorgan compared to hepatopancreas,muscles and gill at the same molting stages of the crab. RXR mRNA of Y-organ in stage D w as significantly( P 0. 05) higher than that of stage AB and stage C. It also had significant difference( P 0. 05) betw een muscles at stage AB and stage E,and there w as no significant difference expression in hepatopancreas and gill tissues in different molting stages.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"1761"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69970410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In order to control Fusarium isolated from shrimp culture environment producing T-2 toxin,the inhibitory effect and antibacterial mechanism of lipopeptides from Bacillus natto on Fusarium was studied in the article.The inhibitory effect was studied through the influence of lipopeptides on the germination and growth of Fusarium spores;and the observation of the ultrastructure of the spores,the mycelium morphology and the mycelium permeability was performed through electron microscope,ordinary microscope and fluorescence microscope respectively to study the antibacterial mechanism.When the concentration of lipopeptides was 1 mg/mL,the inhibition rate on the germination of Fusarium spores reached 78.8%,and the cultivation biomass of Fusarium was 24.5% of the control group without lipopeptides on the third day.Under the condition of the same concentration,the microscopic observation under scanning electron microscope revealed that the spores of Fusarium presented moniliform state under the influence of lipopeptides;ordinary microscope revealed that the mycelia became nonseptate after the treatment of lipopeptides;and fluorescence microscope revealed that lipopeptides could increase the membrane permeability of the Fusarium mycelium.
{"title":"Study on inhibitory effect of lipopeptides from Bacillus Natto on Fusarium isolated from shrimp culture environment producing T-2 toxin","authors":"Huanming Liu, Yaling Wang, Lijun Sun, Defeng Xu, Qi Shi, Jianmeng Liao, Jian-rong Li","doi":"10.3724/SP.J.1231.2013.38297","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38297","url":null,"abstract":"In order to control Fusarium isolated from shrimp culture environment producing T-2 toxin,the inhibitory effect and antibacterial mechanism of lipopeptides from Bacillus natto on Fusarium was studied in the article.The inhibitory effect was studied through the influence of lipopeptides on the germination and growth of Fusarium spores;and the observation of the ultrastructure of the spores,the mycelium morphology and the mycelium permeability was performed through electron microscope,ordinary microscope and fluorescence microscope respectively to study the antibacterial mechanism.When the concentration of lipopeptides was 1 mg/mL,the inhibition rate on the germination of Fusarium spores reached 78.8%,and the cultivation biomass of Fusarium was 24.5% of the control group without lipopeptides on the third day.Under the condition of the same concentration,the microscopic observation under scanning electron microscope revealed that the spores of Fusarium presented moniliform state under the influence of lipopeptides;ordinary microscope revealed that the mycelia became nonseptate after the treatment of lipopeptides;and fluorescence microscope revealed that lipopeptides could increase the membrane permeability of the Fusarium mycelium.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"784"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69964751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38481
Mingyi Cai, Xiande Liu, Ziying Chen, B. Cai, C. Ke
{"title":"Characterization of Pacific abalone(Haliotis discus hannai)karyotype by C-banding and fluorescence in situ hybridization with rDNA","authors":"Mingyi Cai, Xiande Liu, Ziying Chen, B. Cai, C. Ke","doi":"10.3724/SP.J.1231.2013.38481","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38481","url":null,"abstract":"","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"1002"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69967612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Effects of three feeding models on growth rates throughout the whole growth cycle,gonad yield and gonad quality at harvest of the sea urchin Strongylocentrotus intermedius were studied.Six-month-old sea urchins were selected and fed on three diets kelp(E1),kelp+mussel(E2),maize+mussel(E3)to harvest.The test diameter and live body weight of sea urchins were measured every two months.The gonad weight,gonad moisture content and gonad color were measured at the end of the experiment.Results showed that:the feeding model significantly affected(P0.01)the test diameter and live body weight of the sea urchin during its growth stage.The test diameter and live body weight performed as:E2E1E3 among feeding models.The feeding model significantly affected most specific growth rates(SGRs)of the test diameter and live body weight(P0.01),which ranked as:E2≥E1E3.The feeding model significantly affected the gonad yield and gonad quality at harvest(P0.05 or P0.01).E2[female:(7.58±1.21)g,male:(7.74±1.95)g]was the optimal model for the gonad wet weight,followed by E1 [female:(4.50±1.20)g,male:(4.87±1.02)g].E3[female:(2.66±1.02)g,male:(2.32±0.75)g]was the worst model.E2(female:15.07%±1.84%,male:15.83%±3.01%)was the optimal model for the gonad index,followed by E1(female:11.54%±4.94%,male:11.84%±3.70%)and E2(female:12.48%±4.33%,male:12.78%±3.73%).E2 and E1 were the optimal models for the gonad moisture content and gonad color,while E3 was the weakest.Effects of the feeding model on male or female sea urchins were similar.The result suggested that,S.intermedius can grow good test diameter by feeding kelp only.Animal protein can significantly improve the growth rate and gonadal production of S.intermedius.Kelp+mussel model can be applied to improve the culture efficiency of the sea urchin.Maize had no beneficial effect on the promotion of the growth,gonad yield and quality of the sea urchin.Even if animal protein was added,maize+mussel can hardly be an alternative feeding model in aquaculture of S.intermedius.
{"title":"Effects of three feeding models on growth,gonad yield and gonad quality of the sea urchin(Strongylocentrotus intermedius)","authors":"Haisen Zhou, Yaqing Chang, Shibin Luo, Xiaofei Tian, Weijie Zhang, Chong Zhao","doi":"10.3724/SP.J.1231.2013.38485","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38485","url":null,"abstract":"Effects of three feeding models on growth rates throughout the whole growth cycle,gonad yield and gonad quality at harvest of the sea urchin Strongylocentrotus intermedius were studied.Six-month-old sea urchins were selected and fed on three diets kelp(E1),kelp+mussel(E2),maize+mussel(E3)to harvest.The test diameter and live body weight of sea urchins were measured every two months.The gonad weight,gonad moisture content and gonad color were measured at the end of the experiment.Results showed that:the feeding model significantly affected(P0.01)the test diameter and live body weight of the sea urchin during its growth stage.The test diameter and live body weight performed as:E2E1E3 among feeding models.The feeding model significantly affected most specific growth rates(SGRs)of the test diameter and live body weight(P0.01),which ranked as:E2≥E1E3.The feeding model significantly affected the gonad yield and gonad quality at harvest(P0.05 or P0.01).E2[female:(7.58±1.21)g,male:(7.74±1.95)g]was the optimal model for the gonad wet weight,followed by E1 [female:(4.50±1.20)g,male:(4.87±1.02)g].E3[female:(2.66±1.02)g,male:(2.32±0.75)g]was the worst model.E2(female:15.07%±1.84%,male:15.83%±3.01%)was the optimal model for the gonad index,followed by E1(female:11.54%±4.94%,male:11.84%±3.70%)and E2(female:12.48%±4.33%,male:12.78%±3.73%).E2 and E1 were the optimal models for the gonad moisture content and gonad color,while E3 was the weakest.Effects of the feeding model on male or female sea urchins were similar.The result suggested that,S.intermedius can grow good test diameter by feeding kelp only.Animal protein can significantly improve the growth rate and gonadal production of S.intermedius.Kelp+mussel model can be applied to improve the culture efficiency of the sea urchin.Maize had no beneficial effect on the promotion of the growth,gonad yield and quality of the sea urchin.Even if animal protein was added,maize+mussel can hardly be an alternative feeding model in aquaculture of S.intermedius.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"1050"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69967831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38607
Lu Cui-yun, Geng Longwu, Li Chao, Cheng Lei, Sun Xiao-wen, Xu Wei
Barbus capito is an endemic species in the Aral Sea of Uzbekistan.It is well-known for its salt tolerance and fast growth,and is a valuable food source in the region.The species was introduced to China in 2003 and F1 was obtained using artificial propagation in 2010.As an introduced species,B.capito has the relatively limited genetic resources due to the small founder population size.It is very important to effectively protect and utilize the existing gene resources in breeding practice.In this study,24 microsatellite markers were selected to analyze the genetic structure in cultured F1 population including 96 individuals.A total of 74 alleles were detected and the length of fragments ranged from 109 to 367 bp.The number of alleles per locus was from 2 to 5,and the mean was 3.0833.The effective number of alleles(Ne)ranged from 1.144 5 to 4.626 4 with an average of 2.265 6.The observed heterozygosity(Ho)and the expected heterzygosity(He)varied from 0.135 4 to 1.000 0(mean=0.538 4)and from 0.126 9 to 0.788 1(mean=0.487 2),respectively.The value of polymorphic information content(PIC)was between 0.118 3 and 0.749 0 with an average of 0.428 1,and this result indicated that the level of genetic diversity was moderate(0.25≤PIC0.50).The population accorded with Hardy-Weinberg equilibrium checking by χ2 test except 8 markers including B1,B20,B32,B37,B45,B51,B68,and BC43.In addition,the correlation was analyzed between 24 microsatellite markers and growth traits using the GLM procedure of SPSS 19.0.As a result,there were 8 markers that had a significant(P0.05)impact on growth traits.Six markers including B1,B20,B45,B51,B59,and BC38 had a significant(P0.05)impact on body weight,length,height,and thickness.B26 had a significant(P0.05)impact on body weight,height,and thickness.BC3 had a significant(P0.05)impact on body weight and length.Superior genotypes were obtained using Duncan's multiple comparison.
{"title":"Analysis of genetic structure and growth traits in Barbus capito using microsatellite markers","authors":"Lu Cui-yun, Geng Longwu, Li Chao, Cheng Lei, Sun Xiao-wen, Xu Wei","doi":"10.3724/SP.J.1231.2013.38607","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38607","url":null,"abstract":"Barbus capito is an endemic species in the Aral Sea of Uzbekistan.It is well-known for its salt tolerance and fast growth,and is a valuable food source in the region.The species was introduced to China in 2003 and F1 was obtained using artificial propagation in 2010.As an introduced species,B.capito has the relatively limited genetic resources due to the small founder population size.It is very important to effectively protect and utilize the existing gene resources in breeding practice.In this study,24 microsatellite markers were selected to analyze the genetic structure in cultured F1 population including 96 individuals.A total of 74 alleles were detected and the length of fragments ranged from 109 to 367 bp.The number of alleles per locus was from 2 to 5,and the mean was 3.0833.The effective number of alleles(Ne)ranged from 1.144 5 to 4.626 4 with an average of 2.265 6.The observed heterozygosity(Ho)and the expected heterzygosity(He)varied from 0.135 4 to 1.000 0(mean=0.538 4)and from 0.126 9 to 0.788 1(mean=0.487 2),respectively.The value of polymorphic information content(PIC)was between 0.118 3 and 0.749 0 with an average of 0.428 1,and this result indicated that the level of genetic diversity was moderate(0.25≤PIC0.50).The population accorded with Hardy-Weinberg equilibrium checking by χ2 test except 8 markers including B1,B20,B32,B37,B45,B51,B68,and BC43.In addition,the correlation was analyzed between 24 microsatellite markers and growth traits using the GLM procedure of SPSS 19.0.As a result,there were 8 markers that had a significant(P0.05)impact on growth traits.Six markers including B1,B20,B45,B51,B59,and BC38 had a significant(P0.05)impact on body weight,length,height,and thickness.B26 had a significant(P0.05)impact on body weight,height,and thickness.BC3 had a significant(P0.05)impact on body weight and length.Superior genotypes were obtained using Duncan's multiple comparison.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"1121-1128"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69969157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38632
Ling Zhang, Yichen Liu, Yichen Zhang, Yan Sun, Xuyun Gen, Jinsheng Sun
{"title":"Molecular cloning and expression pattern analysis of signal transducer and activator of transcription(Lv-STAT)from the Litopenaeus vannamei","authors":"Ling Zhang, Yichen Liu, Yichen Zhang, Yan Sun, Xuyun Gen, Jinsheng Sun","doi":"10.3724/SP.J.1231.2013.38632","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38632","url":null,"abstract":"","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"1140"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69969773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38670
Y. Zhang, Xinghong Yan
The cross between a red-mutant(R,♀)and a wild type(W,♂)of Pyropia haitanensis in laboratory demonstrated that its gametophytic blades were mainly monoecious.However,sexual observations showed a contrary result in the wild populations of this species.In order to clarify the real sex of the wild blades,some of the key issues related to this discrepancy were studied in this paper.Sequencing analysis of 5.8S rDNA region demonstrated that the monoecious and dioecious blades in wild populations of P.haitanensis were the same species.In nature,the heterozygous conchocelis produced in the cross between a red mutant and the wild type was cultivated,and the conchospores released from mature conchocelis were cultivated into F1 blades.Microscopic observation showed that two parental color sectors(W sector and R sector)and two recombinant color sectors(R′ sector and W′ sector)appeared in F1 blades,and 95.6% of the young blades(about 5 mm in length)were linearly sectored with 2-4 color-sectors.Besides,when those young blades grow up,each mature color-sector in the sectored blade was unisexual(either male or female).Therefore,the color-sectored blades were also the sex-sectored blades.Those findings tallylied with those obtained in laboratory,indicating that neither the position of meiosis nor segregation mode of the parental sex was change in natural conditions.However,during subsequent development of the young blades,one or two basal color-sector(s)was arrested severely.As a result,they formed very small areas on the blade which were hardly discerned by naked eyes.The area composed the rhizoid or basal part of the adult blade which could hardly maturate.Nearly entire blade was derived from one or two top color-sector(s),therefore color-sectored blades dropped to 42.1%.However,the uppermost sector took very large area of the blade and its maturity was much earlier than the middle and basal part.As a result,in the beginning and middle stages of the maturity,almost all the mature parts of the blades were in the upmost sector,and their phenotype showed unisexual even though they were monoecious actually.At the end of maturity of the blades,the middle sectors started to maturate,so the percentage of monoecious blades increased significantly.The results above showed that the wild blades of P.haitanensis in nature were also mainly monoecious.However,the probability for observation of its real sex was greatly reduced because of the uneven development of the tetrad and the asynchronous maturity of the top,middle and base of the blades.Due to these factors,one-time sampled by naked eyes often revealed that the wild blades were dioecious phenotype,with a few exceptions of monoecious phenotype.
{"title":"Observation on tetrad development and formation of sex phenotype of Pyropia haitanensis blades in natural conditions","authors":"Y. Zhang, Xinghong Yan","doi":"10.3724/SP.J.1231.2013.38670","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38670","url":null,"abstract":"The cross between a red-mutant(R,♀)and a wild type(W,♂)of Pyropia haitanensis in laboratory demonstrated that its gametophytic blades were mainly monoecious.However,sexual observations showed a contrary result in the wild populations of this species.In order to clarify the real sex of the wild blades,some of the key issues related to this discrepancy were studied in this paper.Sequencing analysis of 5.8S rDNA region demonstrated that the monoecious and dioecious blades in wild populations of P.haitanensis were the same species.In nature,the heterozygous conchocelis produced in the cross between a red mutant and the wild type was cultivated,and the conchospores released from mature conchocelis were cultivated into F1 blades.Microscopic observation showed that two parental color sectors(W sector and R sector)and two recombinant color sectors(R′ sector and W′ sector)appeared in F1 blades,and 95.6% of the young blades(about 5 mm in length)were linearly sectored with 2-4 color-sectors.Besides,when those young blades grow up,each mature color-sector in the sectored blade was unisexual(either male or female).Therefore,the color-sectored blades were also the sex-sectored blades.Those findings tallylied with those obtained in laboratory,indicating that neither the position of meiosis nor segregation mode of the parental sex was change in natural conditions.However,during subsequent development of the young blades,one or two basal color-sector(s)was arrested severely.As a result,they formed very small areas on the blade which were hardly discerned by naked eyes.The area composed the rhizoid or basal part of the adult blade which could hardly maturate.Nearly entire blade was derived from one or two top color-sector(s),therefore color-sectored blades dropped to 42.1%.However,the uppermost sector took very large area of the blade and its maturity was much earlier than the middle and basal part.As a result,in the beginning and middle stages of the maturity,almost all the mature parts of the blades were in the upmost sector,and their phenotype showed unisexual even though they were monoecious actually.At the end of maturity of the blades,the middle sectors started to maturate,so the percentage of monoecious blades increased significantly.The results above showed that the wild blades of P.haitanensis in nature were also mainly monoecious.However,the probability for observation of its real sex was greatly reduced because of the uneven development of the tetrad and the asynchronous maturity of the top,middle and base of the blades.Due to these factors,one-time sampled by naked eyes often revealed that the wild blades were dioecious phenotype,with a few exceptions of monoecious phenotype.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"871"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69969848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38691
Shengyi Chen, Xinfu Liu, Yongjiang Xu, Zhiliang Liu, Xuezhou Liu, Bao Shi, Yanyan Wang
In order to study the underlying molecular mechanisms for growth of Epinephelus septemfasciatus,the insulin-like growth factorⅠ(IGF-Ⅰ)mature peptide sequence was amplified from E.septemfasciatus liver with RT-PCR method.It was predicted that the mature peptide was composed of 210 base pairs,which encodes 70 amino acid residues and consists of B-C-A-D four functional domains.The matured peptide fragment was subcloned into the expression vector pET-28a and was successfully expressed in E.coli BL21(DE3)cell.The result of SDS-PAGE analysis indicated that the fusion protein expressed in the form of inclusion bodies with molecular weight of 11 ku and maximally amounted to 51.8% of the whole protein in the E.coli cell 3 hours after being induced with IPTG.The purified recombinant protein was obtained through denaturation,purification and refolding.The Western-blotting indicated that the recombinant protein had specifically been recognized by 6×His antibody.The proliferation experiment showed that the purified IGF-Ⅰ fusion protein could significantly promote the proliferation of breast cancer cells MDA231,and this indicated that it has biological activity.
{"title":"Prokaryotic expression and activity analysis of insulin-like growth factor-I from Epinephelus septemfasciatus","authors":"Shengyi Chen, Xinfu Liu, Yongjiang Xu, Zhiliang Liu, Xuezhou Liu, Bao Shi, Yanyan Wang","doi":"10.3724/SP.J.1231.2013.38691","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38691","url":null,"abstract":"In order to study the underlying molecular mechanisms for growth of Epinephelus septemfasciatus,the insulin-like growth factorⅠ(IGF-Ⅰ)mature peptide sequence was amplified from E.septemfasciatus liver with RT-PCR method.It was predicted that the mature peptide was composed of 210 base pairs,which encodes 70 amino acid residues and consists of B-C-A-D four functional domains.The matured peptide fragment was subcloned into the expression vector pET-28a and was successfully expressed in E.coli BL21(DE3)cell.The result of SDS-PAGE analysis indicated that the fusion protein expressed in the form of inclusion bodies with molecular weight of 11 ku and maximally amounted to 51.8% of the whole protein in the E.coli cell 3 hours after being induced with IPTG.The purified recombinant protein was obtained through denaturation,purification and refolding.The Western-blotting indicated that the recombinant protein had specifically been recognized by 6×His antibody.The proliferation experiment showed that the purified IGF-Ⅰ fusion protein could significantly promote the proliferation of breast cancer cells MDA231,and this indicated that it has biological activity.","PeriodicalId":15710,"journal":{"name":"水产学报","volume":"37 1","pages":"1290"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69970254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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