Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38454
X-L Xia, Gui-Ling Wang, Z. Bai, Li Jiale
In order to study genes related to pearl formation in freshwater pearl mussel and their regulation mechanism,a 1 437 bp cDNA sequence of calreticulin gene from Hyriopsis cumingii(HcCRT)was obtained by rapid amplification of cDNA ends(RACE).It consisted of a 231 bp 5′-untranslation region(UTR),a 615 bp 3′UTR and a 591 bp open reading frame(ORF).The inferred amino acids sequence was composed of 196 amino acids,including a signal peptide of 21 amino acids and a mature peptide of 175 amino acids.The molecular weight of the peptide was predicted to be 22.4 ku,with a theoretical isoelectric point of 5.01.Amino acid sequence analysis showed that there was no obvious amino acid sequence of membrane domain.Results of the ProtScale online analysis showed that the protein was hydrophilic protein.Homology analysis indicated that HcCRT amino acid had a conservative sequence of calreticulin family and was highly conserved with Danio rerio(77%),Crassostrea gigas(70%),Pinctada fucata(70%).The sequence analysis showed that HcCRT shared two potential calreticulin family signature motifs with CRT from other species,KHEQNIDCGGGYLKVF and IMFGPDICG.The prediction results of H.cumingii HcCRT protein secondary structure and tertiary structure indicated that the protein contains the alpha helix and beta folding.Real-time quantitative PCR(RT-PCR)showed that HcCRT is expressed in a wide range of tissues including the mantle,blood,gill,foot,liver,kidney,intestine and muscle,with the highest level of transcripts in mantle,followed by blood,while there is rare expression in other tissues.These data suggested that HcCRT might be involved in pearl formation of H.cumingii.This study which was first time to obtain HcCRT gene from H.cumingii played an important role in exploring pearl formation of H.cumingii.
{"title":"Molecular characterization and expression analysis of calreticulin cDNA from Hyriopsis cumingii","authors":"X-L Xia, Gui-Ling Wang, Z. Bai, Li Jiale","doi":"10.3724/SP.J.1231.2013.38454","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38454","url":null,"abstract":"In order to study genes related to pearl formation in freshwater pearl mussel and their regulation mechanism,a 1 437 bp cDNA sequence of calreticulin gene from Hyriopsis cumingii(HcCRT)was obtained by rapid amplification of cDNA ends(RACE).It consisted of a 231 bp 5′-untranslation region(UTR),a 615 bp 3′UTR and a 591 bp open reading frame(ORF).The inferred amino acids sequence was composed of 196 amino acids,including a signal peptide of 21 amino acids and a mature peptide of 175 amino acids.The molecular weight of the peptide was predicted to be 22.4 ku,with a theoretical isoelectric point of 5.01.Amino acid sequence analysis showed that there was no obvious amino acid sequence of membrane domain.Results of the ProtScale online analysis showed that the protein was hydrophilic protein.Homology analysis indicated that HcCRT amino acid had a conservative sequence of calreticulin family and was highly conserved with Danio rerio(77%),Crassostrea gigas(70%),Pinctada fucata(70%).The sequence analysis showed that HcCRT shared two potential calreticulin family signature motifs with CRT from other species,KHEQNIDCGGGYLKVF and IMFGPDICG.The prediction results of H.cumingii HcCRT protein secondary structure and tertiary structure indicated that the protein contains the alpha helix and beta folding.Real-time quantitative PCR(RT-PCR)showed that HcCRT is expressed in a wide range of tissues including the mantle,blood,gill,foot,liver,kidney,intestine and muscle,with the highest level of transcripts in mantle,followed by blood,while there is rare expression in other tissues.These data suggested that HcCRT might be involved in pearl formation of H.cumingii.This study which was first time to obtain HcCRT gene from H.cumingii played an important role in exploring pearl formation of H.cumingii.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69967461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38500
Qiang Liu, Jun-fang Zhou, W. Fang, Y. Wang, Xin-cang Li, Shuai Zhou
In order to evaluate the hazard of chlorpyrifos to the cultured shrimp Litopenaeus vannamei,the lethal effect of the shrimp infected with white spot syndrome virus(WSSV)under the chlorpyrifos stress was tested,while the quantity of WSSV in the gill tissue and acetylcholinesterase acticity in the muscle tissue were analyzed.The 96 h median lethal concentration(LC50)of chlorpyrifos to the shrimp was determined.The results showed that the 96 h LC50 is 0.758 μg/L(0.521-0.987 μg/L),and as time prolonged,the values of LC50 significantly declined,which showed a positive concentration-response relationship.On this basis,the stress concentration of chlorpyrifos of 0.2 μg/L was determined.After the shrimp were exposed to 0.2 μg/L chlorpyrifos for 4 days,they were infected with WSSV by injection.It showed that the cumulate mortality of chlorpyrifos-WSSV treatment(83.33%±4.7%)was significantly higher than that of ethanol-WSSV group(40.00%±0.9%).Meanwhile,the amount of WSSV was also determined by Real-time Relative PCR at 48,72 and 96 h post-injection.WSSV quantity of chlorpyrifos-WSSV treatment was about 4 times that of ethanol-WSSV control at 72 h.WSSV quantity of chlorpyrifos-WSSV treatment was significantly increased at 96 h,which was 4.9 times that of chlorpyrifos-WSSV treatment at 72 h,and 5.9 times that of ethanol-WSSV treatment at 96 h.The acetylcholinesterase(AchE)activity in shrimp muscle tissue under chlorpyrifos stress was 20% lower than that in control group.This showed that the proliferation rate of WSSV in shrimp increased rapidly under chlorpyrifos stress,which raised the mortality rate of the shrimp.
{"title":"Studies of the pathogenicity of WSSV to the cultured shrimp Litopenaeus vannamei under chlorpyrifos stress","authors":"Qiang Liu, Jun-fang Zhou, W. Fang, Y. Wang, Xin-cang Li, Shuai Zhou","doi":"10.3724/SP.J.1231.2013.38500","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38500","url":null,"abstract":"In order to evaluate the hazard of chlorpyrifos to the cultured shrimp Litopenaeus vannamei,the lethal effect of the shrimp infected with white spot syndrome virus(WSSV)under the chlorpyrifos stress was tested,while the quantity of WSSV in the gill tissue and acetylcholinesterase acticity in the muscle tissue were analyzed.The 96 h median lethal concentration(LC50)of chlorpyrifos to the shrimp was determined.The results showed that the 96 h LC50 is 0.758 μg/L(0.521-0.987 μg/L),and as time prolonged,the values of LC50 significantly declined,which showed a positive concentration-response relationship.On this basis,the stress concentration of chlorpyrifos of 0.2 μg/L was determined.After the shrimp were exposed to 0.2 μg/L chlorpyrifos for 4 days,they were infected with WSSV by injection.It showed that the cumulate mortality of chlorpyrifos-WSSV treatment(83.33%±4.7%)was significantly higher than that of ethanol-WSSV group(40.00%±0.9%).Meanwhile,the amount of WSSV was also determined by Real-time Relative PCR at 48,72 and 96 h post-injection.WSSV quantity of chlorpyrifos-WSSV treatment was about 4 times that of ethanol-WSSV control at 72 h.WSSV quantity of chlorpyrifos-WSSV treatment was significantly increased at 96 h,which was 4.9 times that of chlorpyrifos-WSSV treatment at 72 h,and 5.9 times that of ethanol-WSSV treatment at 96 h.The acetylcholinesterase(AchE)activity in shrimp muscle tissue under chlorpyrifos stress was 20% lower than that in control group.This showed that the proliferation rate of WSSV in shrimp increased rapidly under chlorpyrifos stress,which raised the mortality rate of the shrimp.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69968046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38535
Ming Zhou, Bo Liu, X. Ge, Jun Xie, Ruli Chen, Yanting Cui, Jinjuan Wan, Suli Cui, Habte-Tsion H.Michael
The purpose of this study was to investigate the effects of vitamin E(VE)on serum biochemical indices and antioxidant capacity of juvenile Megalobrama amblycephala under acute high temperature stress and recovery.180 healthy fish(45.00±0.50)g were selected and randomly divided into three groups:a control group was fed with basal diet,and two treatment groups fed with basal diet supplemented with 50 and 400 mg/kg VE,respectively.Each group has four replications.After 90 days' feeding trial,all fish were exposed to high temperature stress(32 ℃)for 1 day and recovered at 25 ℃ for 7days in a controlled temperature aquarium.The results showed that the diet supplemented with 50 mg/kg VE significantly increased the activity of intestine glutathione compared with the control group(P0.05)prior to the stress.After high temperature stress,the diet supplemented with 50 mg/kg VE significantly decreased the levels of serum aspertate aminotransferase(ALT)activity and cortisol(COR)content at 1d after stress,reduced the levels of serum alanine aminotransferase(AST)activity,triiodothyronine(T3)and COR content at 7 d recovery after stress(P0.05),and improved the serum alkaline phosphatase(ALP)activity,intestine glutathione(GSH)activity and superoxide dismutase(SOD)activity at 7d recovery after stress(P0.05)compared with the control group(P0.05).The diet supplemented with 400 mg/kg VE significantly reduced the levels of serum ALT and intestine malonaldehyde(MDA)content at 1 day after stress and 7 d recovery after stress,COR content at 7 d recovery after stress(P0.05)and increased the levels of serum ALP and T3 at 1 day after stress concentration compared with the control group(P0.05).So it is suggested that ingestion of a basal diet supplemented with VE(50-400 mg/kg diet)could help regulate the change of blood lipids,improve antioxidant capacity,alleviate the change of blood biochemical parameters in high temperature stress condition,reduce lipid peroxidation,and enhance resistance against high temperature stress in Megalobrama amblycephala.
{"title":"Effects of vitamin E on serum biochemical indexes and antioxidant capacity of Megalobrama amblycephala under acute high temperature stress and recovery","authors":"Ming Zhou, Bo Liu, X. Ge, Jun Xie, Ruli Chen, Yanting Cui, Jinjuan Wan, Suli Cui, Habte-Tsion H.Michael","doi":"10.3724/SP.J.1231.2013.38535","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38535","url":null,"abstract":"The purpose of this study was to investigate the effects of vitamin E(VE)on serum biochemical indices and antioxidant capacity of juvenile Megalobrama amblycephala under acute high temperature stress and recovery.180 healthy fish(45.00±0.50)g were selected and randomly divided into three groups:a control group was fed with basal diet,and two treatment groups fed with basal diet supplemented with 50 and 400 mg/kg VE,respectively.Each group has four replications.After 90 days' feeding trial,all fish were exposed to high temperature stress(32 ℃)for 1 day and recovered at 25 ℃ for 7days in a controlled temperature aquarium.The results showed that the diet supplemented with 50 mg/kg VE significantly increased the activity of intestine glutathione compared with the control group(P0.05)prior to the stress.After high temperature stress,the diet supplemented with 50 mg/kg VE significantly decreased the levels of serum aspertate aminotransferase(ALT)activity and cortisol(COR)content at 1d after stress,reduced the levels of serum alanine aminotransferase(AST)activity,triiodothyronine(T3)and COR content at 7 d recovery after stress(P0.05),and improved the serum alkaline phosphatase(ALP)activity,intestine glutathione(GSH)activity and superoxide dismutase(SOD)activity at 7d recovery after stress(P0.05)compared with the control group(P0.05).The diet supplemented with 400 mg/kg VE significantly reduced the levels of serum ALT and intestine malonaldehyde(MDA)content at 1 day after stress and 7 d recovery after stress,COR content at 7 d recovery after stress(P0.05)and increased the levels of serum ALP and T3 at 1 day after stress concentration compared with the control group(P0.05).So it is suggested that ingestion of a basal diet supplemented with VE(50-400 mg/kg diet)could help regulate the change of blood lipids,improve antioxidant capacity,alleviate the change of blood biochemical parameters in high temperature stress condition,reduce lipid peroxidation,and enhance resistance against high temperature stress in Megalobrama amblycephala.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69968421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38611
Zhu Huaping, Lu Mai-xin, H. Zhang-han, Gao Fengying, Ke Xiaoli, L. Zhigang, Li Qingyong, Li Yujiao
In this study,using the methylation sensitive amplified polymorphism(MSAP) method,we globally assessed the effect of low temperature adaptation on the level and variations of cytosine methylation alterations at CCGG sites of genomic DNA in Nile tilapia(Oreochromis niloticus) strains with good coldresistance characters developed by the successive and directional selection breeding of multi-generations. Total of 849 fragments were identified by 18 pairs of se1ective primer combinations of which 411 and 438 were detected in cold-resistance tilapia strain and the control,respectively. Among these fragments,the methylation sites were 72 and 104,and the corresponding total-methylation levels w ere at 17.52% and 23.74%,respectively. The full methylation sites at the internal Cs were 37 and 65,and the corresponding full methylation levels were at 9. 00% and 14. 84%,respectively,and the hemi-methylation sites at the external Cs were 35 and 39,and the corresponding hemi-methylation levels were at 8.52% and 8.90% in coldresistance tilapia strain and the control,respectively. Further analysis of DNA methylation indicated that a statistically decrease in the overall level of total-methylation levels and both methylation types was detected in cold-resistance tilapia strain compared with the control(decrease at 6.22%,5.84% and 0.38%, respectively),and the changes of DNA methylation pattern were recognized mainly in the full methylation sites. The level of decrease in DNA methylation suggested that the levels of genomic DNA methylation were changed during the successive cold stress of multi-generations in tilapia,and it also indicated that DNA methylation alteration in tilapia treated with cold stress was mainly through de-methylation that occurred in some CCGG sites. All these results implied that the change of DNA methylation was closely associated with cold tolerance of tilapia. The present findings are valuable to further explore the application potential of DNA methylation alteration in tilapia genetic improvement and provide a new method and theoretical basis for fish stress resistance breeding.
{"title":"Effect of low temperature on genomic DNA methylation in Nile tilapia (Oreochromis niloticus)","authors":"Zhu Huaping, Lu Mai-xin, H. Zhang-han, Gao Fengying, Ke Xiaoli, L. Zhigang, Li Qingyong, Li Yujiao","doi":"10.3724/SP.J.1231.2013.38611","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38611","url":null,"abstract":"In this study,using the methylation sensitive amplified polymorphism(MSAP) method,we globally assessed the effect of low temperature adaptation on the level and variations of cytosine methylation alterations at CCGG sites of genomic DNA in Nile tilapia(Oreochromis niloticus) strains with good coldresistance characters developed by the successive and directional selection breeding of multi-generations. Total of 849 fragments were identified by 18 pairs of se1ective primer combinations of which 411 and 438 were detected in cold-resistance tilapia strain and the control,respectively. Among these fragments,the methylation sites were 72 and 104,and the corresponding total-methylation levels w ere at 17.52% and 23.74%,respectively. The full methylation sites at the internal Cs were 37 and 65,and the corresponding full methylation levels were at 9. 00% and 14. 84%,respectively,and the hemi-methylation sites at the external Cs were 35 and 39,and the corresponding hemi-methylation levels were at 8.52% and 8.90% in coldresistance tilapia strain and the control,respectively. Further analysis of DNA methylation indicated that a statistically decrease in the overall level of total-methylation levels and both methylation types was detected in cold-resistance tilapia strain compared with the control(decrease at 6.22%,5.84% and 0.38%, respectively),and the changes of DNA methylation pattern were recognized mainly in the full methylation sites. The level of decrease in DNA methylation suggested that the levels of genomic DNA methylation were changed during the successive cold stress of multi-generations in tilapia,and it also indicated that DNA methylation alteration in tilapia treated with cold stress was mainly through de-methylation that occurred in some CCGG sites. All these results implied that the change of DNA methylation was closely associated with cold tolerance of tilapia. The present findings are valuable to further explore the application potential of DNA methylation alteration in tilapia genetic improvement and provide a new method and theoretical basis for fish stress resistance breeding.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69968984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38624
Yu Lijuan, Y. Jinsong, Ling Peiliang, Cao Shouqi, Cheng Yongxu, Wang Chun, Xia Jun
Aiming to guarantee the whole-process quality control of the Eriocheir sinensis crab seedling breeding and accomplishment of the Shanghai-Taiwan Crab Seedling Transport mission,this paper is based on the technologies referred to as the Internet of Things(IOT)and smart service in which the crab seedling quality dynamic traceability system is researched and developed.Considering the Cross-Strait complex network situation and varied logistics links,several kinds of computing nodes and software patterns are adopted for the purpose of the acquisition of applied information in the system.Meanwhile,it plays an exemplary role in the field of Eriocheir sinensis crab seedling aquiculture for the reason that the quality control,dynamic monitor and traceability and tracking smart service of Cross-Strait crab seedling are brought into effect by means of the uniform data and information exchange between the coordination service space and intelligent resource base.The system has been successfully applied to the whole process of the Shanghai Eriocheir sinensis crab seedling aquaculture and transport to Miaoli,Taiwan in 2013 and rendered the smart quality traceability service to 770 000 seedlings in 695 boxes of 9 batches.The thirty Taiwanese exemplary aquaculture households have realized the quality tracking for the crab seedling with the 100 percent traceability rate of crab seedling boxes and pouches.This has achieved desirable benefits both in politics and economics.
{"title":"Research on dynamic quality traceability system of Eriocheir sinensis seedling based on IOT smart service","authors":"Yu Lijuan, Y. Jinsong, Ling Peiliang, Cao Shouqi, Cheng Yongxu, Wang Chun, Xia Jun","doi":"10.3724/SP.J.1231.2013.38624","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38624","url":null,"abstract":"Aiming to guarantee the whole-process quality control of the Eriocheir sinensis crab seedling breeding and accomplishment of the Shanghai-Taiwan Crab Seedling Transport mission,this paper is based on the technologies referred to as the Internet of Things(IOT)and smart service in which the crab seedling quality dynamic traceability system is researched and developed.Considering the Cross-Strait complex network situation and varied logistics links,several kinds of computing nodes and software patterns are adopted for the purpose of the acquisition of applied information in the system.Meanwhile,it plays an exemplary role in the field of Eriocheir sinensis crab seedling aquiculture for the reason that the quality control,dynamic monitor and traceability and tracking smart service of Cross-Strait crab seedling are brought into effect by means of the uniform data and information exchange between the coordination service space and intelligent resource base.The system has been successfully applied to the whole process of the Shanghai Eriocheir sinensis crab seedling aquaculture and transport to Miaoli,Taiwan in 2013 and rendered the smart quality traceability service to 770 000 seedlings in 695 boxes of 9 batches.The thirty Taiwanese exemplary aquaculture households have realized the quality tracking for the crab seedling with the 100 percent traceability rate of crab seedling boxes and pouches.This has achieved desirable benefits both in politics and economics.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69969545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38630
Zhiguo Dong, Xiaoying Li, Pu-Chang Wang, Wenjin Wang, Qingqi Zhang, B. Yan, Xiaowen Sun
A swimming crab is one of the most economically important edible marine crabs in the East Asia area,and it is widely distributed in the coastal waters of China,Japan and Korea.To assess the level of genetic diversity and genetic variation of swimming crab populations along China coasts,the species from the six geographical populations from China Sea(Dalian,Dongying,Lianyungang,Zhangzhou,Zhoushan and Zhanjiang)were studied based on mitochondrial D-loop whole gene sequences.There were 1 141 bp,185 variation sites and 129 parsimony informative sites in D-loop whole gene sequences.And there were 48 haplotypes among 60 swimming crab individuals.Nucleotide diversity index and Haplotype diverstity index together revealed a high genetic diversity and low selective effect for swimming crab populations in China Sea.The genetic differential index FST of total six populations was 0.189 72,which meant that the genetic differentiation among populations was moderate and significant(P0.05)and the inferred differentiation time was about(196.8-260.5)×103 years.Low gene flow indicated that there were genetic differentiations among the fifteen groups,except between LYG and DY,ZJ,ZZ,respectively,in addition between ZJ and ZZ(Nem5).Especially,there were pronounced levels of genetic differentiation between ZS and the others and a moderate genetic differentiation between DL and LYG,DY,ZJ and ZZ,respectively.The genetic distance had no significant correlation with geographical distance,so there may be more intricate reasons in population occurrence and spread.
{"title":"Genetic diversity and genetic differentiation of swimming crab (Portunus trituberculatus) from six geographical populations of China Sea based on mitochondrial D-loop gene","authors":"Zhiguo Dong, Xiaoying Li, Pu-Chang Wang, Wenjin Wang, Qingqi Zhang, B. Yan, Xiaowen Sun","doi":"10.3724/SP.J.1231.2013.38630","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38630","url":null,"abstract":"A swimming crab is one of the most economically important edible marine crabs in the East Asia area,and it is widely distributed in the coastal waters of China,Japan and Korea.To assess the level of genetic diversity and genetic variation of swimming crab populations along China coasts,the species from the six geographical populations from China Sea(Dalian,Dongying,Lianyungang,Zhangzhou,Zhoushan and Zhanjiang)were studied based on mitochondrial D-loop whole gene sequences.There were 1 141 bp,185 variation sites and 129 parsimony informative sites in D-loop whole gene sequences.And there were 48 haplotypes among 60 swimming crab individuals.Nucleotide diversity index and Haplotype diverstity index together revealed a high genetic diversity and low selective effect for swimming crab populations in China Sea.The genetic differential index FST of total six populations was 0.189 72,which meant that the genetic differentiation among populations was moderate and significant(P0.05)and the inferred differentiation time was about(196.8-260.5)×103 years.Low gene flow indicated that there were genetic differentiations among the fifteen groups,except between LYG and DY,ZJ,ZZ,respectively,in addition between ZJ and ZZ(Nem5).Especially,there were pronounced levels of genetic differentiation between ZS and the others and a moderate genetic differentiation between DL and LYG,DY,ZJ and ZZ,respectively.The genetic distance had no significant correlation with geographical distance,so there may be more intricate reasons in population occurrence and spread.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69969695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38712
Feilin Ma, Qinqin Lu, Chuanming Hu, Tao Zhang, W. Zhou, Jiafu Li, Yin-Yin Deng, Jianyi Zhu
Ten cultivated strains of Pyropia yezoensis were studied in this research in order to identify the effects of the strain difference and the harvesting time difference on the fatty acid composition and content of P. yezoensis. The results showed that the fatty acid composition of these ten P. yezonsis samples was not affected; however,the content of each fatty acid was affected and the difference among ten samples was significant. The saturated fatty acids(SFAs) w ere mainly composed of 13:0,16∶0,18∶0 with 16∶0 as the highest component(about 20%). The unsaturated fatty acids(UFAs) included 18∶1n-9,18∶2n-6,18∶3n-3,20∶1n-9,20∶2n-7,20∶3n-7,20∶4n-6( AA),20∶5n-3(EPA) and the major componet w as EPA(about 50%). Samples harvested during the low est w ater temperature period(February) contained the highest total fatty acids and EPA concentration; the result indicates that the low water temperature has advantages in accumulating fatty acids especially UFAs of P. yezoenis. P. yezoensis is rich in UFAs(especially EPA). The n-6/n-3 ratios ranged from 4.9 to 7.7 during the whole study period,and this range is consistent with the recommended diet ratio of WHO/FAO.
{"title":"The variation in fatty acids composition and content of ten Pyropia yezoensis cultivars during different harvest time periods","authors":"Feilin Ma, Qinqin Lu, Chuanming Hu, Tao Zhang, W. Zhou, Jiafu Li, Yin-Yin Deng, Jianyi Zhu","doi":"10.3724/SP.J.1231.2013.38712","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38712","url":null,"abstract":"Ten cultivated strains of Pyropia yezoensis were studied in this research in order to identify the effects of the strain difference and the harvesting time difference on the fatty acid composition and content of P. yezoensis. The results showed that the fatty acid composition of these ten P. yezonsis samples was not affected; however,the content of each fatty acid was affected and the difference among ten samples was significant. The saturated fatty acids(SFAs) w ere mainly composed of 13:0,16∶0,18∶0 with 16∶0 as the highest component(about 20%). The unsaturated fatty acids(UFAs) included 18∶1n-9,18∶2n-6,18∶3n-3,20∶1n-9,20∶2n-7,20∶3n-7,20∶4n-6( AA),20∶5n-3(EPA) and the major componet w as EPA(about 50%). Samples harvested during the low est w ater temperature period(February) contained the highest total fatty acids and EPA concentration; the result indicates that the low water temperature has advantages in accumulating fatty acids especially UFAs of P. yezoenis. P. yezoensis is rich in UFAs(especially EPA). The n-6/n-3 ratios ranged from 4.9 to 7.7 during the whole study period,and this range is consistent with the recommended diet ratio of WHO/FAO.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69969890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38659
Wei Liu, Yaqing Chang, Jun Ding
The experiment studied the change of enzymes in mild and acute drop of temperature.The activities of CAT,SOD,POD and the content of MDA in "Shuiyuan No.1" sea cucumber and cultured sea cucumber were analyzed.The results show that:(1)In terms of mild drop in temperature,the activity of CAT were trend to decreased and then increased in "Shuiyuan No.1" sea cucumber and Dalian cultured sea cucumber,the activities of CAT were 402.78-424.32 U/mL and 409.81-430.08 U/mL in "Shuiyuan No.1" sea cucumber and Dalian cultured sea cucumber;the activity of SOD and POD first increased and then decreased and then increased in "Shuiyuan No.1" sea cucumber and Dalian cultured sea cucumber;the activities of SOD were 0.019-0.086 U/mL and 0.014-0.069 U/mL in "Shuiyuan No.1" sea cucumber and Dalian cultured sea cucumber,the activities of POD were 0.000 45-0.000 89 U/mL and 0.000 24-0.000 66 U/mL in "Shuiyuan No.1" sea cucumber and Dalian cultured sea cucumber;the activity of MDA first increased and then reduced in "Shuiyuan No.1" sea cucumber and Dalian cultured sea cucumber,the content of MDA were 5.83-9.79 nmol/mL and 7.57-9.90 nmol/mL in "Shuiyuan No.1" sea cucumber and Dalian cultured sea cucumber.(2)In terms of acute drop in temperature,the activity of CAT,SOD and MDA first increased and then reduced in "Shuiyuan No.1" sea cucumber and Dalian cultured sea cucumber,the activities of CAT were 382.51-409.71 U/mL and 383.86-399.42 U/mL in "Shuiyuan No.1" sea cucumber and Dalian cultured sea cucumber;the activities of SOD were 0.023-0.038 U/mL and 0.025-0.035 U/mL in "Shuiyuan No.1" sea cucumber and Dalian cultured sea cucumber;the content of MDA were 5.83-9.79 nmol/ml and 5.57-9.90 nmol/mL in "Shuiyuan No.1" sea cucumber and Dalian cultured sea cucumber;the activity of POD were first increased and then decreased and then increased again to reduce,the activities of POD were 0.000 20-0.000 70 U/mL and 0.000 18-0.000 40 U/mL in "Shuiyuan No.1" sea cucumber and Dalian cultured sea cucumber.(3)Both the activities of CAT,SOD,POD and the content of MDA were changed in the different ways of dropping temperature and the changing in "Shuiyuan No.1" sea cucumber was more sensitive than in Dalian cultured sea cucumber in the terms of mild and acute drop in temperature.The activities of CAT and POD was higher in "Shuiyuan No.1" sea cucumber than in Dalian cultured sea cucumber;the content of MDA was larger in the terms of acute drop than in the terms of mild drop in temperature.These results revealed that there are different immune responses to sea cucumber in different drops of temperature patterns."Shuiyuan No.1" sea cucumber was more sensitive and has higher capacity of antioxidant than Dalian cultured sea cucumber in the low temperature environment.
{"title":"A preliminary study of effects of mild and acute drop of temperature on serum non-specific immunity in“Shuiyuan No.1”sea cucumber populations and the cultured sea cucumber populations","authors":"Wei Liu, Yaqing Chang, Jun Ding","doi":"10.3724/SP.J.1231.2013.38659","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38659","url":null,"abstract":"The experiment studied the change of enzymes in mild and acute drop of temperature.The activities of CAT,SOD,POD and the content of MDA in \"Shuiyuan No.1\" sea cucumber and cultured sea cucumber were analyzed.The results show that:(1)In terms of mild drop in temperature,the activity of CAT were trend to decreased and then increased in \"Shuiyuan No.1\" sea cucumber and Dalian cultured sea cucumber,the activities of CAT were 402.78-424.32 U/mL and 409.81-430.08 U/mL in \"Shuiyuan No.1\" sea cucumber and Dalian cultured sea cucumber;the activity of SOD and POD first increased and then decreased and then increased in \"Shuiyuan No.1\" sea cucumber and Dalian cultured sea cucumber;the activities of SOD were 0.019-0.086 U/mL and 0.014-0.069 U/mL in \"Shuiyuan No.1\" sea cucumber and Dalian cultured sea cucumber,the activities of POD were 0.000 45-0.000 89 U/mL and 0.000 24-0.000 66 U/mL in \"Shuiyuan No.1\" sea cucumber and Dalian cultured sea cucumber;the activity of MDA first increased and then reduced in \"Shuiyuan No.1\" sea cucumber and Dalian cultured sea cucumber,the content of MDA were 5.83-9.79 nmol/mL and 7.57-9.90 nmol/mL in \"Shuiyuan No.1\" sea cucumber and Dalian cultured sea cucumber.(2)In terms of acute drop in temperature,the activity of CAT,SOD and MDA first increased and then reduced in \"Shuiyuan No.1\" sea cucumber and Dalian cultured sea cucumber,the activities of CAT were 382.51-409.71 U/mL and 383.86-399.42 U/mL in \"Shuiyuan No.1\" sea cucumber and Dalian cultured sea cucumber;the activities of SOD were 0.023-0.038 U/mL and 0.025-0.035 U/mL in \"Shuiyuan No.1\" sea cucumber and Dalian cultured sea cucumber;the content of MDA were 5.83-9.79 nmol/ml and 5.57-9.90 nmol/mL in \"Shuiyuan No.1\" sea cucumber and Dalian cultured sea cucumber;the activity of POD were first increased and then decreased and then increased again to reduce,the activities of POD were 0.000 20-0.000 70 U/mL and 0.000 18-0.000 40 U/mL in \"Shuiyuan No.1\" sea cucumber and Dalian cultured sea cucumber.(3)Both the activities of CAT,SOD,POD and the content of MDA were changed in the different ways of dropping temperature and the changing in \"Shuiyuan No.1\" sea cucumber was more sensitive than in Dalian cultured sea cucumber in the terms of mild and acute drop in temperature.The activities of CAT and POD was higher in \"Shuiyuan No.1\" sea cucumber than in Dalian cultured sea cucumber;the content of MDA was larger in the terms of acute drop than in the terms of mild drop in temperature.These results revealed that there are different immune responses to sea cucumber in different drops of temperature patterns.\"Shuiyuan No.1\" sea cucumber was more sensitive and has higher capacity of antioxidant than Dalian cultured sea cucumber in the low temperature environment.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69970250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38822
Zhou Wei, C. Ping, Qiu Chenggong, Zhou Jiankai, Guo Xiaoge, Wang Danli, Yunlong Zhao
To analyze the expression pattern of Doublesex( dsx) of Daphnia pulex in different reproductive modes,w e extracted total RNA of different reproductive modes ' Daphnia pulex. Using RT-PCR and Quantitative real-time PCR analyzed dsx expression in different reproductive modes' daphnias. In addition,w e synthesized DIG labeled RNA probe of dsx by in vitro transcription and used w hole mount in situ hybridization researching dsx location expression in different reproductive modes' daphnia. Results show ed that dsx in male and female had no gender specificity of alternative splicing,only a same transcription. Realtime PCR show ed dsx highest expression level w as in male,w hich w as parthenogenetic female's 2. 6 times and 4. 9 times that of the female. They had significant difference( P 0. 05) and gender differences in expression each other. Whole mount in situ hybridization found that dsx gene w as expressed in the somatic tissure in different from reproductive modes ' daphnia,but the difference in quantity and parts. The expression also show ed the gender differences in male and female. In male the signals w ere revealed in the first antenna,the first thoracic limb and the compound eye. The parthenogenetic female and sexual female corresponding sites' expressions w ere relatively w eak. These suggested that Doublesex gene likely played a significant role in alternation of generation and sexual differentiation.
为了分析水蚤双性基因(dsx)在不同生殖模式下的表达规律，我们提取了水蚤不同生殖模式的总RNA。采用RT-PCR和实时荧光定量PCR分析了dsx在不同繁殖模式水蚤中的表达。此外，我们通过体外转录合成了dsx的DIG标记RNA探针，并利用孔载原位杂交技术研究了dsx在不同繁殖方式水蚤中的位置表达。结果表明，雄性和雌性的dsx没有选择性剪接的性别特异性，只有相同的转录。real - time PCR结果显示，was在雄性中表达量最高，在孤雌雌性中表达量最高。6乘以4。是雌性的9倍。差异有统计学意义(P < 0.05)。05)和性别表达差异。全株原位杂交发现，dsx基因在不同生殖方式水蚤的体细胞组织中均有表达，但表达量和表达部位不同。这个表达也显示了男性和女性的性别差异。在雄性中，信号显示在第一天线、第一胸肢和复眼。孤雌雌和有性雌对应位点的表达相对较弱。这表明双性基因可能在世代交替和性别分化中起着重要作用。
{"title":"Doublesex gene expression of Daphnia pulex during different modes of reproduction by using whole mount in situ hybridization and quantitative expression","authors":"Zhou Wei, C. Ping, Qiu Chenggong, Zhou Jiankai, Guo Xiaoge, Wang Danli, Yunlong Zhao","doi":"10.3724/SP.J.1231.2013.38822","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38822","url":null,"abstract":"To analyze the expression pattern of Doublesex( dsx) of Daphnia pulex in different reproductive modes,w e extracted total RNA of different reproductive modes ' Daphnia pulex. Using RT-PCR and Quantitative real-time PCR analyzed dsx expression in different reproductive modes' daphnias. In addition,w e synthesized DIG labeled RNA probe of dsx by in vitro transcription and used w hole mount in situ hybridization researching dsx location expression in different reproductive modes' daphnia. Results show ed that dsx in male and female had no gender specificity of alternative splicing,only a same transcription. Realtime PCR show ed dsx highest expression level w as in male,w hich w as parthenogenetic female's 2. 6 times and 4. 9 times that of the female. They had significant difference( P 0. 05) and gender differences in expression each other. Whole mount in situ hybridization found that dsx gene w as expressed in the somatic tissure in different from reproductive modes ' daphnia,but the difference in quantity and parts. The expression also show ed the gender differences in male and female. In male the signals w ere revealed in the first antenna,the first thoracic limb and the compound eye. The parthenogenetic female and sexual female corresponding sites' expressions w ere relatively w eak. These suggested that Doublesex gene likely played a significant role in alternation of generation and sexual differentiation.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69971238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1231.2013.38138
Jiangping Tao, Zhou Xin-qi, Liu Hongbo, Li Huanzhang, Y. Jian
To confirm whether there is anadromous estuarine tapertail anchovy Coilia nasus now in Poyang Lake,an electron probe microanalyzer(EPMA)was applied to analyze the element Sr and Ca microchemistry patterns in otoliths of two individuals collected from Xingzi area of the lake.Results of line transect analysis showed that the ratios of Sr∶ Ca×1 000 in the otolith of the individual with a shorter supramaxilla(PYCB02)were consistently low(1.66±0.54),indicating that it experienced in freshwater habitat during its whole life history.Another individual with a longer supramaxilla(PYCE05)had a complex Sr/Ca ratio pattern which can be divided into three phases(1.82±0.50,5.79±0.91,and 4.14±0.88),suggesting it experienced not only the freshwater,but also the seawater and brackish water during different stages in its life history.All these differences could be well confirmed by the results of X-ray mapping analysis of EPMA.The whole otolith of PYCB02 presented a uniform bluish pattern(low Sr level)from the core to the outermost regions.In contrast,the otolith of PYCE05 showed a multi-color pattern.There were greenish(moderate Sr level),yellowish(moderate Sr level),or even reddish(high Sr level)in outer regions adjacent to the bluish central regions.Results of the two different microchemistry patterns obtained by both the line transect and mapping analyses confirmed objectively,for the first time,the coexistence of resident and anadromous forms of Coilia nasus in Poyang Lake and suggested that Coilia nasus is still possible to migrate anadromously for spawning into Poyang Lake,which is about 800 km away from the river mouth of Yangtze River.These aforementioned microchemistry patterns will provide important information not only to more effectively evaluate and protect the resources of Coilia nasus,but also to rationally plan and guide future ecological economic development in Poyang Lake.
{"title":"Two microchemistry patterns in otoliths of Coilia nasus from Poyang Lake, China.","authors":"Jiangping Tao, Zhou Xin-qi, Liu Hongbo, Li Huanzhang, Y. Jian","doi":"10.3724/SP.J.1231.2013.38138","DOIUrl":"https://doi.org/10.3724/SP.J.1231.2013.38138","url":null,"abstract":"To confirm whether there is anadromous estuarine tapertail anchovy Coilia nasus now in Poyang Lake,an electron probe microanalyzer(EPMA)was applied to analyze the element Sr and Ca microchemistry patterns in otoliths of two individuals collected from Xingzi area of the lake.Results of line transect analysis showed that the ratios of Sr∶ Ca×1 000 in the otolith of the individual with a shorter supramaxilla(PYCB02)were consistently low(1.66±0.54),indicating that it experienced in freshwater habitat during its whole life history.Another individual with a longer supramaxilla(PYCE05)had a complex Sr/Ca ratio pattern which can be divided into three phases(1.82±0.50,5.79±0.91,and 4.14±0.88),suggesting it experienced not only the freshwater,but also the seawater and brackish water during different stages in its life history.All these differences could be well confirmed by the results of X-ray mapping analysis of EPMA.The whole otolith of PYCB02 presented a uniform bluish pattern(low Sr level)from the core to the outermost regions.In contrast,the otolith of PYCE05 showed a multi-color pattern.There were greenish(moderate Sr level),yellowish(moderate Sr level),or even reddish(high Sr level)in outer regions adjacent to the bluish central regions.Results of the two different microchemistry patterns obtained by both the line transect and mapping analyses confirmed objectively,for the first time,the coexistence of resident and anadromous forms of Coilia nasus in Poyang Lake and suggested that Coilia nasus is still possible to migrate anadromously for spawning into Poyang Lake,which is about 800 km away from the river mouth of Yangtze River.These aforementioned microchemistry patterns will provide important information not only to more effectively evaluate and protect the resources of Coilia nasus,but also to rationally plan and guide future ecological economic development in Poyang Lake.","PeriodicalId":15710,"journal":{"name":"Journal of Fisheries of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69963009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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