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Influence of biofertilizer produced using drumstick (Moringa oleifera L.) unused parts on the growth performance of two leafy vegetables 利用鸡腿未使用部分生产生物肥料对两种叶菜生长性能的影响
Q4 Veterinary Pub Date : 2023-04-30 DOI: 10.18006/2023.11(2).280.289
Hatsadong Chanthanousone, T. T. Phan, Co Quang Nguyen, The Dieu Thi Nguyen, Hien T T Pham, H. Truong
The non-edible parts of Moringa oleifera, such as stems, branches or leaf petioles, have often been discarded while the leaves are consumed as a vegetable or are used to produce organic fertilizer. This study aimed to determine the optimal conditions for producing Moringa organic fertilizer (MOF) from previously unused parts and to compare these fertilizers with cow manure and bio-organic fertilizer. Seventy kilograms of the unused Moringa parts were blended with fifty kilograms of manure, 0.2 kilogram of Trichoderma-based product and two kilograms of superphosphate. The mixture was incubated at different intervals, including 5, 7 or 9 weeks. Next, the effects of MOF on the growth, yield, ascorbic acid content and Brix of lettuce and mustard spinach were also determined and compared with other organic fertilizers (cow manure and bio-organic fertilizer). Results of the study revealed that 25 tons per ha of MOF were significantly superior to those treated with cow manure and bio-organic fertilizer in the case of vegetable yields. Further, 7 weeks of MOF incubation was found suitable to produce an optimal yield during the various incubation period. These results suggested that the Moringa non-edible parts can make organic fertilizer and enhance growth, yield, and leafy vegetable production.
辣木的非食用部分,如茎、枝或叶柄,通常在叶子作为蔬菜食用或用于生产有机肥料时被丢弃。本研究旨在确定用以前未使用的部分生产辣木有机肥料(MOF)的最佳条件,并将这些肥料与牛粪和生物有机肥料进行比较。将70公斤未使用的辣木部分与50公斤粪肥、0.2公斤木霉基产品和2公斤过磷酸钙混合。混合物以不同的时间间隔孵育,包括5、7或9周。其次,还测定了MOF对生菜和芥菜菠菜生长、产量、抗坏血酸含量和白利糖度的影响,并与其他有机肥料(牛粪和生物有机肥料)进行了比较。研究结果表明,在蔬菜产量方面,每公顷25吨MOF显著优于牛粪和生物有机肥处理的MOF。此外,发现7周的MOF孵育适合在不同的孵育期内产生最佳产量。这些结果表明,辣木的非食用部分可以制成有机肥料,提高生长、产量和叶菜产量。
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引用次数: 0
Positive impacts of integrating flaxseed meal as a potential feed supplement in livestock and poultry production: Present scientific understanding 亚麻籽粉作为一种潜在的饲料补充剂在畜禽生产中的积极影响:目前的科学认识
Q4 Veterinary Pub Date : 2023-04-30 DOI: 10.18006/2023.11(2).264.279
Athira Rajan, Devika V M, Aysha Shabana, Nayana Krishnan, Krishnapriya N Anil, Rohith Krishnan, Baby Shajini Y, Bhadra S Dev, A. J, M. S, Amrithendhu V R, Sandip Chakraborty, Hitesh Chopra, A. Dey, A. Sharma, K. Dhama, Deepak Chandran
When it comes to food and fiber production, flaxseed (Linum usitatissimum) has been around the longest. Oil makes up over 41% of a flaxseed's total weight; of that, more than 70% is polyunsaturated. Protein, dietary fiber, α-linolenic acid (ALA), flaxseed gum, and many other beneficial compounds are abundant in flaxseed meal (FSM). There is as much as 30% crude protein in FSM. Therefore, FSM can serve as a source of excellent protein for livestock. FSM increases the efficiency and effectiveness of livestock and poultry farming. FSM can be used as an essential protein feed component in cattle and poultry farming, boosting production and profitability. Because it contains anti-nutritional ingredients such as cyanogenic glycosides, tannins, phytic acid, oxalic acid and an anti-vitamin B6 factor, the use of FSM in livestock and poultry diets is restricted. Animal nutritionists have recently shown a growing interest in reducing anti-nutritional elements and boosting FSM's nutritional value. Recently, fermented FSM has been used to feed cattle and poultry; hence its dietary benefits have not yet been fully assessed. The present article, therefore, addresses the chemical make-up, bioactive components, anti-nutritional aspects, and positive impacts of FSM in livestock and poultry production.
说到食物和纤维的生产,亚麻籽(Linum usitatissimum)的历史最悠久。油占亚麻籽总重量的41%以上;其中,超过70%是多不饱和的。蛋白质、膳食纤维、α-亚麻酸(ALA)、亚麻籽胶和许多其他有益化合物在亚麻籽粕(FSM)中含量丰富。FSM的粗蛋白质含量高达30%。因此,FSM可作为家畜优质蛋白质的来源。FSM提高了畜禽养殖的效率和效益。FSM可以作为牛和家禽养殖中必不可少的蛋白质饲料成分,提高产量和盈利能力。由于FSM含有抗营养成分,如氰苷、单宁、植酸、草酸和抗维生素B6因子,因此在畜禽饲料中使用FSM受到限制。动物营养学家最近对减少抗营养成分和提高FSM的营养价值表现出越来越大的兴趣。近年来,发酵的FSM已被用于饲养牛和家禽;因此,其饮食益处尚未得到充分评估。因此,本文讨论了FSM在畜禽生产中的化学组成、生物活性成分、抗营养方面和积极影响。
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引用次数: 2
Prospective nutritional, therapeutic, and dietary benefits of camel milk making it a viable option for human consumption: Current state of scientific knowledge 骆驼奶的营养、治疗和饮食益处使其成为人类消费的可行选择:目前的科学知识状况
Q4 Veterinary Pub Date : 2023-04-30 DOI: 10.18006/2023.11(2).236.250
S. S, Vasukhi S M, Shreya Ramesh, Rajakumari R, Abhijith A S, A. S, Gautam Prakash, Raida, Abhirami V Nair, Aishwarya Prashanth, P. M, Sandip Chakraborty, Hitesh Chopra, A. Dey, A. Sharma, K. Dhama, Deepak Chandran
For over five thousand years, people in Asia and Africa have known about the health benefits of camel milk. Thus, it is used not only as a food source but also as a medicine. The similarities between camel milk and human milk have been scientifically proven. Camel milk is unique among ruminant milk because it is high in vitamins C and E and low in sugar and cholesterol. Still, it contains a wide variety of beneficial minerals (including sodium, potassium, iron, copper, zinc, and magnesium), besides being rich in several nutrients, including monounsaturated and polyunsaturated fatty acids, serum albumin, lactoferrin, immunoglobulins, lysozyme and the hormone insulin. Because of these components, many medical professionals now recommend camel milk as a treatment for various human ailments. It has been demonstrated to be effective in treating gastrointestinal issues, Type 1 diabetes, and food allergies. As a bonus, camel milk has been utilized to cure autism, lower cholesterol, prevent psoriasis, heal inflammation, aid tuberculosis patients, boost the body's natural defences, and impede the spread of cancer cells. Those who have problems digesting lactose may still be able to tolerate it. Conversely, camel milk can also help reduce an excessively high bilirubin, globulin, and granulocyte count. Drinking camel milk does not affect the erythrocyte sedimentation rate, hemoglobin concentration, and leukocyte count. The proteins in camel milk have an adequate ratio of critical amino acids. Immunoglobulins, which fight disease, are contained inside, and their small size allows antigens to penetrate and boosts the immune system's efficacy. This article highlights the health benefits and medicinal uses of camel milk.
5000多年来,亚洲和非洲的人们都知道骆驼奶对健康的好处。因此,它不仅被用作食物来源,而且还被用作药物。骆驼奶和母乳之间的相似性已经得到科学证明。骆驼奶在反刍动物奶中是独一无二的,因为它富含维生素C和E,糖和胆固醇含量低。尽管如此,它还含有多种有益矿物质(包括钠、钾、铁、铜、锌和镁),此外还富含多种营养素,包括单不饱和和多不饱和脂肪酸、血清白蛋白、乳铁蛋白、免疫球蛋白、溶菌酶和激素胰岛素。由于这些成分,许多医学专业人士现在推荐骆驼奶作为治疗各种人类疾病的药物。它已被证明对治疗胃肠道问题、1型糖尿病和食物过敏有效。此外,骆驼奶还被用于治疗自闭症、降低胆固醇、预防牛皮癣、治疗炎症、帮助结核病患者、增强身体的自然防御能力以及阻止癌症细胞的传播。那些消化乳糖有问题的人可能仍然能够耐受乳糖。相反,骆驼奶也有助于降低过高的胆红素、球蛋白和粒细胞计数。饮用骆驼奶不会影响红细胞沉降率、血红蛋白浓度和白细胞计数。骆驼奶中的蛋白质含有足够比例的关键氨基酸。免疫球蛋白含有对抗疾病的免疫球蛋白,其体积小,可以使抗原渗透并增强免疫系统的效力。这篇文章强调了骆驼奶的健康益处和药用价值。
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引用次数: 0
An Integrative Approach Towards Recommending Farming Solutions for Sustainable Agriculture 为可持续农业推荐农业解决方案的综合方法
Q4 Veterinary Pub Date : 2023-04-30 DOI: 10.18006/2023.11(2).306.315
Veena Ghuriani, Jyotsna Talreja Wassan, Pragya Deolal, Vidushi Sharma, Dimpy Dalal, A. Goyal
Sustainable Agriculture is rapidly emerging as an important discipline to meet societal needs for food and other resources by adopting paradigms of conserving natural resources while maximizing productivity benefits. This paper proposes an integrative methodological approach for critically analyzing Precision Farming (PF) paradigms and Zero Budget Natural Farming (ZBNF), providing sustainable farming solutions and achieving productivity and profitability. This paper analyses the productivity of crops in PF using various machine learning (ML) algorithms based on different soil and climatic factors to identify sustainable agricultural practices for maximizing crop production and generating recommendations for the farmers. When implemented on the collected dataset from various Indian states, the Random Forest (RF) model produced the best results with an AUC-ROC of 95.7%. The Juxtaposition of ZBNF and non-ZBNF is evinced. ZBNF is statistically (p<0.05) observed to be a cost-efficient and more profitable alternative. The impact of ZBNF on soil microbial diversity and micro-nutrients is also discussed.
可持续农业正在迅速成为一门重要学科,通过采用保护自然资源的模式,同时最大限度地提高生产力,来满足社会对粮食和其他资源的需求。本文提出了一种综合方法论方法,用于批判性分析精准农业(PF)范式和零预算自然农业(ZBNF),提供可持续农业解决方案,实现生产力和盈利能力。本文基于不同的土壤和气候因素,使用各种机器学习(ML)算法分析了PF中作物的生产力,以确定可持续的农业实践,最大限度地提高作物产量,并为农民提供建议。当在从印度各州收集的数据集上实现时,随机森林(RF)模型产生了最好的结果,AUC-ROC为95.7%。ZBNF和非ZBNF的并置得到了证明。ZBNF在统计学上(p<0.05)被认为是一种成本效益高、利润更高的替代品。还讨论了ZBNF对土壤微生物多样性和微量养分的影响。
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引用次数: 0
Isolation and production of polyhydroxybutyrate (PHB) from Bacillus pumilus NMG5 strain for bioplastic production and treatment of wastewater from paper factories 短小芽孢杆菌NMG5菌株分离生产聚羟基丁酸酯(PHB)用于生物塑料生产和造纸废水处理
Q4 Veterinary Pub Date : 2023-04-30 DOI: 10.18006/2023.11(2).351.358
H. Minh, Ngo Duy Thai, Tran Vu Anh Khoa, Nguyen Thi Ngoc Thao, J. Sichaem
Polyhydroxybutyrate (PHB) has the potential to replace traditional plastics and limit environmental pollution caused by plastic waste. This study combined wastewater treatment with PHB production to reduce costs. Bacteria capable of synthesizing PHB were isolated from paper mill wastewater and identified using Matrix Assisted Laser Desorption/Ionization–Time of Flight (MALDI-TOF) mass spectrometry and 16S rRNA gene analysis. Bacillus pumilus NMG5 strain was found to have a good yield in modified Nutrient Broth culture, reaching 42.28% of dry biomass. The PHB product was analyzed using FTIR spectroscopy and 1H NMR spectroscopy. The bacterial strain was also tested for its ability to treat paper mill wastewater, and it showed impressive results in terms of biochemical oxygen demand (COD), total nitrogen, and total phosphorus, with efficiencies of 95.93%, 79.36%, and 83.55%, respectively. The study found that wastewater treatment combined with PHB production was a promising solution to reduce costs and limit environmental pollution. The bacterial strain B. pumilus NMG5 had a high yield of PHB, and the PHB product was of high quality, as confirmed by FTIR and 1H NMR spectroscopy. Furthermore, the bacterial strain showed impressive results in treating paper mill wastewater with high COD, total nitrogen, and total phosphorus efficiencies. These results suggest that this harmless bacterium could be used in paper mill wastewater treatment systems to produce PHB, providing a sustainable and environmentally friendly solution.
聚羟基丁酸酯(PHB)有潜力取代传统塑料,限制塑料垃圾对环境的污染。本研究将废水处理与PHB生产相结合,以降低成本。从造纸厂废水中分离出能够合成PHB的细菌,并使用基质辅助激光解吸/电离-飞行时间(MALDI-TOF)质谱和16S rRNA基因分析进行鉴定。短小芽孢杆菌NMG5菌株在改良营养肉汤培养基中产量较高,达到干生物量的42.28%。使用FTIR光谱和1H NMR光谱分析PHB产物。该菌株还测试了其处理造纸废水的能力,在生化需氧量(COD)、总氮和总磷方面显示出令人印象深刻的结果,效率分别为95.93%、79.36%和83.55%。研究发现,废水处理与PHB生产相结合是降低成本和限制环境污染的一个很有前途的解决方案。菌株B.pumilus NMG5具有高的PHB产率,并且PHB产物具有高质量,如FTIR和1H NMR光谱所证实的。此外,该菌株在处理具有高COD、总氮和总磷效率的造纸废水方面表现出令人印象深刻的结果。这些结果表明,这种无害的细菌可以用于造纸废水处理系统中生产PHB,提供一种可持续和环保的解决方案。
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引用次数: 0
Determination of carbendazim residues in Moroccan tomato samples using local enzyme-linked immunosorbent assay and comparison with liquid chromatography 用酶联免疫吸附法和液相色谱法测定摩洛哥番茄样品中多菌灵残留量
Q4 Veterinary Pub Date : 2023-04-30 DOI: 10.18006/2023.11(2).339.350
N. Bellemjid, A. Moussaif, M. El Mzibri, A. Mesfioui, A. Iddar
The fungicide carbendazim (CBZ) is not approved for agricultural uses in some countries but is still used by many farmers due to its effectiveness. For this reason, in previous work of the same authors, they developed a competitive enzyme immunoassay (ELISA) using rabbit polyclonal antibodies to detect CBZ. This study aimed to validate this in-house ELISA after extraction with methanol for CBZ analysis in tomato samples, and the results were compared with the conventional high-performance liquid chromatography (HPLC) method after QuEChERS extraction. The results showed that both ELISA and HPLC methods have good repeatability, reproducibility and high precision with a good variation verified by principal components analysis (PCA). ANOVA tested the detection limit (LOD), and quantification limit (LOQ), and the values for ELISA (LOD = 0.026± 0.001 µg/L and LOQ = 0.083 ± 0.003 µg/L) were significantly lower than those obtained by HPLC (LOD = 0.61 ± 0.02 µg/L and LOQ = 1.85 ± 0.07 µg/L). ELISA and HPLC were used for analyzing CBZ in 100 Moroccan tomato samples. These two methods detected the presence of CBZ above the Maximum Residue Limit (MRL) level in 9 samples. However, the presence of the  CBZ was detected in the 79 samples by ELISA and quantified in 66 samples. In contrast, the presence of CBZ was detected in 57 and quantified in 35 samples by HPLC. These results showed that the ELISA system coupled with a simple methanol extraction is much more sensitive than HPLC after QuEChERS extraction.
杀菌剂多菌灵(CBZ)在一些国家未被批准用于农业用途,但由于其有效性,许多农民仍在使用。因此,在同一作者之前的工作中,他们开发了一种使用兔多克隆抗体检测CBZ的竞争性酶免疫分析法(ELISA)。本研究旨在验证甲醇提取后的室内ELISA法对番茄样品中CBZ的分析效果,并与传统的高效液相色谱(HPLC)提取后的结果进行比较。结果表明,ELISA和HPLC方法重复性好,重现性好,精密度高,主成分分析(PCA)验证变异性好。单因素方差分析(ANOVA)检验了检测限(LOD)和定量限(LOQ),结果表明,ELISA法的检测限(LOD = 0.026±0.001µg/L, LOQ = 0.083±0.003µg/L)显著低于HPLC法的检测限(LOD = 0.61±0.02µg/L, LOQ = 1.85±0.07µg/L)。采用酶联免疫吸附法和高效液相色谱法对100份摩洛哥番茄样品中的CBZ进行了分析。这两种方法在9个样品中检测到CBZ超过最大残留限量(MRL)。然而,在79份样品中检测到CBZ的存在,并在66份样品中进行了定量。相反,在57份样品中检测到CBZ的存在,并在35份样品中进行HPLC定量。结果表明,经QuEChERS提取后,酶联免疫吸附法(ELISA)加甲醇提取比高效液相色谱法(HPLC)更灵敏。
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引用次数: 0
Donkey milk: chemical make-up, biochemical features, nutritional worth, and possible human health benefits - Current state of scientific knowledge 驴奶:化学成分、生物化学特征、营养价值和可能对人类健康的益处-科学知识现状
Q4 Veterinary Pub Date : 2023-04-30 DOI: 10.18006/2023.11(2).251.263
Deepa P R, Divya Dharshini C S, Bhadra S Dev, Jyotika Jayan, Harisankaran P S, N. Rajan, Karthik S, Nandhana J P, Athulya K G, P. M, Sandip Chakraborty, Hitesh Chopra, A. Dey, A. Sharma, K. Dhama, Deepak Chandran
Milk and milk derivatives are widely consumed because of their high nutritional density. Donkey milk and milk products have been consumed since ancient times. The use of donkey milk in the human diet is gaining popularity. The abundance of antibacterial components and protective elements in donkey milk sets it apart from the milk of other animals. Like human milk, donkey milk has low fat, high lactose, and low casein/whey protein ratio. Donkey milk whey protein's anti-proliferative properties imply lung cancer treatment. Alpha-lactalbumin, a type of protein, has been found to have antiviral, anticancer, and anti-stress properties. Donkey milk, like human milk, includes a low amount of casein and a smaller quantity of beta-lactoglobulin than cow milk. Donkey milk is an alternative for newborns with cow milk protein allergy and lactose intolerance since it has a higher amount of lactose, improves palatability, and prevents allergies. Osteogenesis, arteriosclerosis therapy, cardiac rehabilitation, accelerated aging, and hypocholesterolemic diets are some areas where donkey milk is beneficial. Since it contains probiotic lactobacilli strains, fermented beverages can be made with donkey milk. Donkey milk moisturizes skin due to its high vitamin, mineral, and polyunsaturated fatty acid content. The chemical makeup and potential therapeutic benefits of donkey milk warrant additional research. This has led to a rise in interest in producing dairy goods derived from donkey milk. Donkey milk has been used to make cheese, ice cream, milk powder, and even some experimental useful fermented drinks. The present article summarises what we know about donkey milk's chemical makeup, biological functions, nutritional worth, and possible human health benefits.
牛奶和牛奶衍生物因其高营养密度而被广泛消费。自古以来,人们就食用驴奶和奶制品。在人类饮食中使用驴奶越来越受欢迎。驴奶中丰富的抗菌成分和保护元素使它有别于其他动物的奶。和人奶一样,驴奶脂肪含量低,乳糖含量高,酪蛋白/乳清蛋白比例低。驴奶乳清蛋白的抗增殖特性意味着肺癌的治疗。α -乳清蛋白是一种蛋白质,已被发现具有抗病毒、抗癌和抗应激的特性。驴奶和人奶一样,酪蛋白含量较低,β -乳球蛋白含量也比牛奶少。驴奶是对牛奶蛋白过敏和乳糖不耐症新生儿的替代选择,因为驴奶含有更多的乳糖,改善了适口性,并防止过敏。成骨、动脉硬化治疗、心脏康复、加速衰老和降低胆固醇饮食是驴奶有益的一些领域。由于驴奶含有益生菌乳酸菌菌株,所以可以用驴奶制作发酵饮料。驴奶富含维生素、矿物质和多不饱和脂肪酸,可以滋润皮肤。驴奶的化学成分和潜在的治疗益处值得进一步研究。这使得人们对用驴奶生产乳制品的兴趣大增。驴奶被用来制作奶酪、冰淇淋、奶粉,甚至一些实验用的发酵饮料。本文综述了驴奶的化学成分、生物功能、营养价值以及可能对人体健康的益处。
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引用次数: 1
Growth and development patterns in Mustard (Brassica spp.) as influenced by sowing time 播期对芥菜生长发育规律的影响
Q4 Veterinary Pub Date : 2023-04-30 DOI: 10.18006/2023.11(2).325.338
Sushan Chowhan, Majharul Islam, M. Rana, Nazmul Alam Khan, Md. Khan Jahan Ali, Nasir Uddin Ahmed, M. Rahman
Mustard is Bangladesh's leading oil crop, produced only during the winter (rabi) season. The sowing date is a key factor determining mustard's optimum growth and development. Because of global warming, gradual changes in season and weather parameters over time is creating a challenge in mustard cultivation. Thus, the present investigation assessed the role of different planting dates on several modern mustard varieties to disclose the optimum growth indicators necessary for elevated biological yield (BY) and harvest index (HI). Three planting times, viz. 31st October (D1), 10th November (D2),  20th November (D3) and six varieties viz. Binasarisha-4 (V1), Binasarisha-9 (V2), Binasarisha-10 (V3), BARI Sarisha-14 (V4), BARI Sarisha-16 (V5), BARI Sarisha-17 (V6) were put on a replicated factorial randomized complete block design (RCBD) during rabi 2019 at BINA Sub-station farm, Magura. At the final harvest stage, outcomes depicted that highest and lowest total dry mass (g/plant) was produced by treatment D3× V5 (64.03) and D1× V1 (15.34), maximum and minimum absolute growth rate (mg/plant/day) by D1× V5 (2389.10) and D2× V1 (184.50), most and least relative growth rate (mg/g/day) in D1× V4 (53.34) and D2× V1 (3.55), maximum and least crop growth rate (g/m2/day) with D1× V3 (55.60) and  D3× V4 (20.04). BY was the peak (8.13, 8.71, 8.77 t/ha) under all plantings (D1, D2, D3) with V5 variety, but HI (44.96%) was most in variety V4 with D2 sowing. Therefore, correlation studies showed a significant positive relationship between biological yield and harvest index. Overall, BARI Sarisha-16 performed well in all three sowing times, and remarkably, BY was rising with delayed planting in the case of Binasarisha-9, Binasarisha-10, and BARI Sarisha-14. This implies that delayed planting might not hamper yield but boost yield to some extent.
芥菜是孟加拉国主要的油料作物,只在冬季(rabi)季节生产。播期是决定芥菜最佳生长发育的关键因素。由于全球变暖,随着时间的推移,季节和天气参数的逐渐变化给芥菜种植带来了挑战。因此,本研究评估了不同种植日期对几种现代芥菜品种的作用,以揭示提高生物产量(BY)和收获指数(HI)所需的最佳生长指标。采用重复因子随机完全区设计(RCBD),于2019年10月31日(D1)、11月10日(D2)、11月20日(D3) 3个种植期和6个品种,即Binasarisha-4 (V1)、Binasarisha-9 (V2)、Binasarisha-10 (V3)、BARI Sarisha-14 (V4)、BARI Sarisha-16 (V5)、BARI Sarisha-17 (V6)。在最后收获阶段,d3xv5(64.03)和d1xv1(15.34)处理的总干质量(g/株)最高和最低,d1xv5(2389.10)和d2xv1(184.50)处理的绝对生长率(mg/株/d)最高和最低,d1xv4(53.34)和d2xv1(3.55)处理的相对生长率(mg/g/d)最高和最低,d1xv3(55.60)和d3xv4(20.04)处理的生长率(g/m2/d)最高和最低。在D1、D2、D3三个品种中,V5品种的BY最高(8.13、8.71、8.77 t/ha),而在V4品种中,D2品种的HI最高(44.96%)。因此,相关研究表明,生物产量与收获指数呈显著正相关。总体而言,BARI Sarisha-16在所有三个播期均表现良好,并且值得注意的是,Binasarisha-9、Binasarisha-10和BARI Sarisha-14的BY随着播期的推迟而上升。这意味着推迟种植可能不会影响产量,而是在一定程度上提高产量。
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引用次数: 0
A Study of the Photodegradation Carbofuran and its Metabolites in Paddy Water Samples 光降解稻田水中呋喃丹及其代谢产物的研究
Q4 Veterinary Pub Date : 2023-04-30 DOI: 10.18006/2023.11(2).394.404
Nurul Syuhada Haji Baharudin, H. Ahmad
Rice fields are one of the agricultural sectors in Malaysia that are heavily pesticide-treated. This study aimed to determine how carbofuran degrades in paddy water and how carbofuran metabolites such as carbofuran-phenol and 3-keto carbofuran reacted during the degradation. The experiment was conducted in two distinct conditions: the first water sample was exposed to sunlight, while the second water sample remained in the dark. During the 56 days of observation, the study discovered carbofuran decomposed slowly in both conditions. The water sample exposed to sunlight showed a faster degradation rate (0.04/day carbofuran) than the water kept in the dark (0.0186/day). The results also demonstrated that photolysis and hydrolysis enhanced the carbofuran degradation in the water. Both 3-keto carbofuran and carbofuran-phenol were detected as metabolites with low concentration levels, ranging from 0.03±0.301 to 0.23±0.142 ppm. These metabolites are considered 'emerging pollutants' as they can be detected in the environment and may post-treat as much as the parent compounds themselves. Hence, this study is trying to fill the research gap to assess the route and rate of carbofuran and its transformation products.  
稻田是马来西亚经过大量农药处理的农业部门之一。本研究旨在确定呋喃丹在稻田水中的降解过程,以及呋喃丹代谢产物如呋喃丹酚和3-酮基呋喃丹在降解过程中的反应。实验在两个不同的条件下进行:第一个水样暴露在阳光下,而第二个水样保持在黑暗中。在56天的观察中,研究发现呋喃丹在这两种条件下都分解缓慢。暴露在阳光下的水样表现出比黑暗中的水(0.0186/天)更快的降解速率(0.04/天的呋喃丹)。结果还表明,光解和水解增强了水中呋喃丹的降解。3-酮基呋喃丹和呋喃丹酚均为低浓度代谢物,浓度范围为0.03±0.301至0.23±0.142ppm。这些代谢物被认为是“新出现的污染物”,因为它们可以在环境中检测到,并且可能与母体化合物本身一样多地进行后处理。因此,本研究试图填补研究空白,以评估呋喃丹及其转化产物的路线和转化率。
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引用次数: 0
Morphology and DNA marker for distinguishing Paphiopedilum hangianum and Paphiopedilum emersonii from Vietnam 越南汉足兰和埃默氏足兰的形态学和DNA标记
Q4 Veterinary Pub Date : 2023-04-30 DOI: 10.18006/2023.11(2).423.435
Yen Nguyen Thi Hai, Quang Ngo Xuan, Trong Nguyen Dinh, Phat Do Tien, Mau Chu Hoang
Genus Paphiopedilum has species having lovely flowers which are incredibly attractive to everyone. Their ornamental and commercial value caused over-collection and illegal poaching and trade. Due to these reasons, nowadays, the Venus slipper orchids are facing to deplete in nature. Therefore, it is important to consider these species conservation. Mainly, it is necessary to prioritize the identification and phylogenetic analysis methods of the genus Paphiopedilum which includes many species with similar morphological characteristics. Consequently, it isn't easy to distinguish the identical species of this genus when the plants are young or not yet fully flowering. Therefore, this study aimed to distinguish two Paphiopedilum species, i.e. P. hangianum and P. emersonii, which have similar morphological characteristics, through comparative morphological analysis and differences in DNA barcoding sequences. To solve the problem associated with species identifications, a morphological comparison table was created with the four DNA sequence markers matK, rbcL, rpoC1 and trnH-psbA. The results of the morphological analysis showed that P. hangianum and P. emersonii are significantly different from each other in the flower's characteristics. While the difference in leaf morphology of both selected species is found very little, it is also distinguishable upon careful comparison. Moreover, the DNA barcoding indicator gave accurate and rapid distinctions between the two species, even when     the plants are young or without flowers. Furthermore, this DNA barcoding can establish an evolutionary relationship between the two selected species and the other species of the genus Paphiopedilum. The results of this study also suggested that the indicator trnH-psbA is a suitable marker for distinguishing these two species and can be applied for the phylogenetic analysis of the genus Paphiopedilum in Vietnam.
兜兰属有一些品种开着可爱的花,对每个人都非常有吸引力。它们的观赏价值和商业价值导致了过度收集和非法偷猎和贸易。由于这些原因,如今,金星拖鞋兰在自然界中面临枯竭。因此,考虑保护这些物种是很重要的。主要是有必要优先考虑兜兰属的鉴定和系统发育分析方法,兜兰属包括许多具有相似形态特征的物种。因此,当植物还年轻或尚未完全开花时,很难区分该属的相同物种。因此,本研究旨在通过比较形态学分析和DNA条形码序列的差异,区分具有相似形态特征的两个兜足目物种,即汉盖兜足目和埃默氏兜足目。为了解决与物种鉴定相关的问题,用四个DNA序列标记matK、rbcL、rpoC1和trnH-psbA创建了一个形态学比较表。形态分析结果表明,汉葛和埃默松在花的性状上存在显著差异。虽然两个选定物种的叶片形态差异很小,但经过仔细比较,也可以区分。此外,DNA条形码指示器可以准确快速地区分这两个物种,即使是在植物年轻或没有花的时候。此外,这种DNA条形码可以在所选的两个物种和兜兰属的其他物种之间建立进化关系。本研究结果还表明,trnH-psbA是区分这两个物种的合适标记,可用于越南兜兰属的系统发育分析。
{"title":"Morphology and DNA marker for distinguishing Paphiopedilum hangianum and Paphiopedilum emersonii from Vietnam","authors":"Yen Nguyen Thi Hai, Quang Ngo Xuan, Trong Nguyen Dinh, Phat Do Tien, Mau Chu Hoang","doi":"10.18006/2023.11(2).423.435","DOIUrl":"https://doi.org/10.18006/2023.11(2).423.435","url":null,"abstract":"Genus Paphiopedilum has species having lovely flowers which are incredibly attractive to everyone. Their ornamental and commercial value caused over-collection and illegal poaching and trade. Due to these reasons, nowadays, the Venus slipper orchids are facing to deplete in nature. Therefore, it is important to consider these species conservation. Mainly, it is necessary to prioritize the identification and phylogenetic analysis methods of the genus Paphiopedilum which includes many species with similar morphological characteristics. Consequently, it isn't easy to distinguish the identical species of this genus when the plants are young or not yet fully flowering. Therefore, this study aimed to distinguish two Paphiopedilum species, i.e. P. hangianum and P. emersonii, which have similar morphological characteristics, through comparative morphological analysis and differences in DNA barcoding sequences. To solve the problem associated with species identifications, a morphological comparison table was created with the four DNA sequence markers matK, rbcL, rpoC1 and trnH-psbA. The results of the morphological analysis showed that P. hangianum and P. emersonii are significantly different from each other in the flower's characteristics. While the difference in leaf morphology of both selected species is found very little, it is also distinguishable upon careful comparison. Moreover, the DNA barcoding indicator gave accurate and rapid distinctions between the two species, even when     the plants are young or without flowers. Furthermore, this DNA barcoding can establish an evolutionary relationship between the two selected species and the other species of the genus Paphiopedilum. The results of this study also suggested that the indicator trnH-psbA is a suitable marker for distinguishing these two species and can be applied for the phylogenetic analysis of the genus Paphiopedilum in Vietnam.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47584798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Journal of Experimental Biology and Agricultural Sciences
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