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Chemical and antibacterial properties of Baccharis dracunculifolia DC essential oils from different regions of Brazil 来自巴西不同地区的龙葵DC精油的化学和抗菌特性
IF 3 3区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2022-08-19 DOI: 10.1080/10412905.2022.2103043
Erika da Silva Monteiro, Kelly de Sousa Monteiro, Patrícia da Silva Montes, Cláudio Augusto Gomes Camara, M. M. Moraes, C. Fagg, Daniel Oliveira Freire, Eliana Fortes Gris, I. C. Rodrigues da Silva, L. C. Sá-Barreto, Daniela Castilho Orsi
ABSTRACT This study aimed to evaluate the chemical and antibacterial properties of the essential oil of B. dracunculifolia from three regions of Brazil: Midwest, Southeast and South region.   The major compounds of essential oil from Midwest region were (E)-nerolidol (28.15%) and spathulenol (17.68%), both oxygenated sesquiterpenes. And the major compounds of essential oils from Southeast and South regions were (E)-nerolidol (12.75–13.95%), bicyclogermacrene (10.76–14.21%) and limonene (10.49–19.36%). The three essential oils showed antibacterial activity for both Gram-positive and Gram-negative bacteria tested, with MIC values from 0.03 to 0.15 mg/mL and MBC values from 0.05 to 0.30 mg/mL. The B. dracunculifolia essential oil from the Midwest region of Brazil presented higher values of oxygenated sesquiterpenes and was more effective on all tested bacterial strains, with lower and statistically significant MBC values, compared to the oils from the Southeast and South regions of Brazil
摘要:本研究旨在评价巴西中西部、东南部和南部三个地区龙葵精油的化学成分和抑菌性能。中西部地区挥发油的主要成分为(E)-神经樟醇(28.15%)和spathulenol(17.68%),均为含氧倍半萜。东南、南部地区主要挥发油成分为(E)-橙花醇(12.75 ~ 13.95%)、双环革烯(10.76 ~ 14.21%)和柠檬烯(10.49 ~ 19.36%)。3种精油对革兰氏阳性菌和革兰氏阴性菌均有抑菌活性,MIC值为0.03 ~ 0.15 mg/mL, MBC值为0.05 ~ 0.30 mg/mL。与产自巴西东南部和南部地区的精油相比,产自巴西中西部地区的龙葵精油含氧倍半萜含量较高,对所有被试菌株都更有效,MBC值较低且具有统计学意义
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引用次数: 2
Seasonal variation of Thymus piperella L. essential oil composition. Relationship among γ-terpinene, p-cymene and carvacrol 麝香精油成分的季节变化。γ-萜烯、对伞花烃和香芹酚的关系
IF 3 3区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2022-08-13 DOI: 10.1080/10412905.2022.2103191
J. Llorens-Molina, S. Vacas, Natalia Escrivá, M. Verdeguer
ABSTRACT A wild population of Thymus piperella L. was monitored for two years in order to correlate phenological stages and meteorological data with yield and, essential oil (EO) composition. The plant material was extracted by hydrodistillation and the EO was submitted to GC-MS and GC-FID analysis. To achieve reliable quantification, relative response factors were applied. The results showed a progressive increase in the proportion of carvacrol according to the sunlight duration and temperature increase, until reaching a maximum at the beginning of flowering stage (52.9 % and 41.0 % in 2018 and 2019, respectively). A simultaneous decrease was found in the amount of p-cymene (8.2 % and 12.8 % respectively) in the same stage, whereas -terpinene showed a similar evolution to that observed for carvacrol but with lower values (3.3 % - 14.6 %). From statistical analysis, significant changes in the relative proportions of carvacrol and its metabolic precursors: γ-terpinene and p-cymene were found. They have been explained based on the proposed metabolic pathways in the literature. On the other hand, when relating the changes in the EO profiles with rainfall data, a strong influence of the water supply on the evolution of the chemical composition was observed.
摘要对野生百里香(thyymus piperella L.)种群进行了为期两年的监测,以确定其物候期和气象数据与产量和精油成分的相关性。采用加氢蒸馏法提取植物材料,并进行气相色谱-质谱和气相色谱- fid分析。为了获得可靠的定量,应用了相关响应因子。结果表明,随着日照时间的延长和温度的升高,香芹酚的比例逐渐增加,并在开花初期达到最大值(2018年和2019年分别为52.9%和41.0%)。在同一时期,对伞花烃的含量同时下降(分别为8.2%和12.8%),而-萜烯的含量与香芹酚的含量变化相似,但含量较低(3.3% ~ 14.6%)。通过统计分析,发现香芹酚及其代谢前体γ-萜烯和对伞花烃的相对比例发生了显著变化。它们是根据文献中提出的代谢途径来解释的。另一方面,当将EO剖面的变化与降雨资料联系起来时,观察到供水对化学成分演变的强烈影响。
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引用次数: 0
Cytotoxic activity and antioxidant effects of Origanum onites essential oil and its two major contents, carvacrol and p-cymene on human colorectal (HCT116) and hepatocelluler carcinoma (HepG2) cell lines 土豆泥精油及其两种主要成分香芹酚和对伞花素对人大肠癌(HCT116)和肝癌(HepG2)细胞的细胞毒活性和抗氧化作用
IF 3 3区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2022-08-11 DOI: 10.1080/10412905.2022.2107101
E. Becer, E. M. Altundag, K. Başer, H. Vatansever, M. Bayar
ABSTRACT Origanum onites L. (O. onites) is one of the cultivated Origanum species in Turkey. The aim of this study was to determine the cytotoxicity and cellular antioxidant effects of O. onites essential oil, carvacrol and p-cymene on HCT116 and HepG2 cells. O. onites essential oil components were analyzed by GC/MS and GC/FID. Cytotoxicity was analyzed via the MTT assay in HCT116 and HepG2 cells. Cellular antioxidant activities of O. onites essential oil, carvacrol and p-cymene were determined with DCFH-DA method. GC/MS analysis identified carvacrol (%78.4) as the main constituent of O. onites essential oil. 400 µg/ml concentration of O. onites essential oil, carvacrol and p-cymene were found to be active against HCT116 and HepG2 cells. Also, carvacrol showed higher cellular antioxidant activity than O. onites essential oil and p-cymene. We conclude that O. onites essential oil and carvacrol may be a candidate for pharmaceutical products in treatment of cancer.
土一枝(Origanum onites L., O. onites)是土耳其的一种人工培育的土一枝。本研究的目的是研究石杉精油、香芹酚和对花香烃对HCT116和HepG2细胞的细胞毒性和细胞抗氧化作用。采用GC/MS、GC/FID等分析方法对黄芪挥发油成分进行了分析。MTT法检测HCT116和HepG2细胞的细胞毒性。采用DCFH-DA法测定了龙葵挥发油、香芹酚和对花香烃的细胞抗氧化活性。GC/MS分析鉴定出香芹酚(% 78.4%)为龙葵挥发油的主要成分。400µg/ml浓度的龙葵精油、香芹酚和对花香烃对HCT116和HepG2细胞均有抑制作用。此外,香芹酚的细胞抗氧化活性也高于石楠精油和对花香烃。我们得出结论,龙葵精油和香芹酚可能是治疗癌症的候选药物产品。
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引用次数: 9
Chemical composition, in vitro antifungal activity, DFT, molecular docking and molecular dynamics simulation studies of the essential oil from Anvillea gracinii subsp. radiata (Coss. & Durieu) Anderb 黄花百合挥发油的化学成分、体外抗真菌活性、DFT、分子对接及分子动力学模拟研究。放射虫纲(输出电容。&杜里厄·安德布
IF 3 3区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2022-08-08 DOI: 10.1080/10412905.2022.2109767
L. Oucheikh, Omar Ou-ani, Soumia Moujane, A. Ansari, A. Oubair, M. Znini
ABSTRACT This study describes the chemical composition and the in vitro antifungal activity of the essential oil (EO) of Anvillea gracinii subsp. radiata aerial parts against three phytopathogenic using the poisoned food (PF) and the volatile assay (VA) techniques. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS) allowed the identification of 35 compounds which cis 8-acetoxychrysantenyl acetate (cis8ACA) (30.8%), cis-chrysanthenyl acetate (15.5%), 6-oxocyclonerolidol (14.9%) and τ-cadinol (7.4%) are the main constituents. In VA test, the minimum inhibitory concentrations (MIC) are 0.50 µL/mL for B. cinerea and P. expansum and 1.00 µL/mL for R. stolonifer, but they are 0.125 µL/mL for B. cinerea and 0.25 µL/mL for other fungi using PF technique. Molecular docking and molecular dynamics (MD) simulations revealed that cis8ACA has the smallest docking energy (-8.9 kcal.mol-1) indicating its high affinity to bind to the active site of the C14DM thereby forming a stable cis8ACA -C14DM complex.
摘要本文研究了黄花Anvillea gracinii subsp.挥发油的化学成分及体外抗真菌活性。利用中毒食品(PF)和挥发性测定(VA)技术对三种植物病原进行辐射试验。气相色谱(GC)和气相色谱/质谱(GC- ms)鉴定出35种化合物,其中顺式8-乙酰氧基乙酸菊酯(cis8ACA)占30.8%,顺式乙酸菊酯(15.5%),6-氧环醇(14.9%)和τ-cadinol(7.4%)为主要成分。在VA试验中,对灰绿芽孢杆菌和绿芽孢杆菌的最低抑菌浓度(MIC)为0.50µL/mL,对匍匐发霉的最低抑菌浓度为1.00µL/mL,而对灰绿芽孢杆菌的最低抑菌浓度为0.125µL/mL,对其他真菌的最低抑菌浓度为0.25µL/mL。分子对接和分子动力学(MD)模拟显示,cis8ACA具有最小的对接能量(-8.9 kcal.mol-1),表明cis8ACA与C14DM的活性位点结合具有较高的亲和力,从而形成稳定的cis8ACA -C14DM复合物。
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引用次数: 2
An in vitro study of the origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities 芫荽精油治疗慢性扁桃体炎的体外研究:抗菌、抗生物膜、抗氧化和细胞毒活性
IF 3 3区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2022-08-06 DOI: 10.1080/10412905.2022.2107103
M. Kostić, Marija Ivanov, Tatjana Marković, Snežana Sanković Babić, L. Barros, R. Calhelha, M. Soković, A. Ćirić
ABSTRACT The chemical composition and biological activity of essential oils (EOs) from leaves of Origanum minutiflorum O. Schwarz & P.H.Davis, and seeds of Coriandrum sativum L. were investigated. Chemical analyses of EOs were performed and the major components were carvacrol (81.5%) and linalool (69.6%), respectively. The antimicrobial activity was assessed against several bacteria originating from the tonsillar tissue. Activities of EOs against Staphylococcus aureus biofilm were investigated, as well as the effect of the mixture of these EOs and antibiotics against the pathogen. The antioxidant activity of both EOs was determined by TBARS assay, and examined wild oregano EO showed better activity. Also, cytotoxicity of EOs was evaluated in vitro and both EOs showed potential to inhibit further proliferation of tumor cells. This study reported for the first time the effects of EOs on chronic tonsillitis causative pathogens, supporting their role as a natural bioactive therapeutic agent with possible antimicrobial applications.
摘要:研究了Origanum minutiflorum O. Schwarz和P.H.Davis叶片和芫荽(Coriandrum sativum L.)种子精油的化学成分和生物活性。对精油进行化学分析,主要成分为香芹酚(81.5%)和芳樟醇(69.6%)。对来自扁桃体组织的几种细菌进行了抗菌活性评估。研究了EOs对金黄色葡萄球菌生物膜的活性,以及这些EOs与抗生素混合使用对金黄色葡萄球菌生物膜的作用。TBARS法测定两种提取物的抗氧化活性,野生牛至提取物表现出较好的抗氧化活性。此外,体外评估了EOs的细胞毒性,两种EOs都显示出抑制肿瘤细胞进一步增殖的潜力。本研究首次报道了EOs对慢性扁桃体炎病原体的作用,支持其作为一种天然生物活性治疗剂的作用,并可能应用于抗菌领域。
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引用次数: 1
Effect of different extraction techniques on the composition of essential oil isolated from fennel (Foeniculum vulgare Mill.) rhizome 不同提取工艺对茴香根茎精油成分的影响
IF 3 3区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2022-08-06 DOI: 10.1080/10412905.2022.2107102
D. Cvetković, J. Stanojević, Natalija Djordjević, Ivana T. Karabegović, L. Stanojević, B. Pavlić, B. Danilović
ABSTRACT The aim of the presented work was to compare the efficiency of innovative (solvent-free microwave extraction, microwave-assisted hydrodistillation and supercritical CO2 extraction) extraction techniques vs. conventional Clevenger hydrodistillation of essential oil (EO) from poorly studied fennel rhizome. It was found that innovative extraction techniques enhance the EOs/extracts isolation, save time and exclude solvents providing green ecologically technologies. Solvent-free microwave extraction proved to be the most efficient with limonene and (E)-anethole as dominant compounds in concentration of 12.34 ± 0.31 and 35.63 ± 1.51 mg/ml, respectively, as well as with the largest number of isolated and identified compounds. Primary aroma compounds were identified by two direct headspace techniques – static headspace extraction (HSS) and headspace solid phase microextraction (HS-SPME) confirming limonene and (E)-anethole as dominant compounds in primary aroma mixture with 53.7 ± 2.02% and 30.9 ± 1.43% (HSS) and 69.8 ± 2.80% and 13.1 ± 0.11% (HS-SPME), respectively.
摘要本研究的目的是比较创新的(无溶剂微波萃取、微波辅助加氢蒸馏和超临界CO2萃取)提取技术与传统的Clevenger加氢蒸馏技术对茴香根茎精油(EO)的提取效率。研究发现,创新的提取技术提高了EOs/提取物的分离,节省了时间,并排除了溶剂,提供了绿色生态技术。结果表明,无溶剂微波提取效果最好,柠檬烯和(E)-茴香脑为优势化合物,萃取浓度分别为12.34±0.31和35.63±1.51 mg/ml,分离和鉴定的化合物数量最多。采用静态顶空萃取(HSS)和顶空固相微萃取(HS-SPME)两种直接顶空技术鉴定了主要香气成分,确定柠檬烯和(E)-茴香脑为主要香气成分,分别占53.7±2.02%和30.9±1.43% (HSS)和69.8±2.80%和13.1±0.11% (HS-SPME)。
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引用次数: 3
Preparation, characterization, and antimicrobial activity of cinnamon essential oil and cinnamaldehyde nanoemulsions 肉桂精油和肉桂醛纳米乳的制备、表征及抗菌活性研究
IF 3 3区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2022-08-05 DOI: 10.1080/10412905.2022.2107100
Mai M. Badr, Mohamad E. I. Badawy, Nehad E. M. Taktak
ABSTRACT Cinnamomum verum essential oil and its main component, cinnamaldehyde, were incorporated into nanoemulsions to improve their stability and activity. Oil-in-water (O/W) nanoemulsions were prepared using the high-energy ultra-sonication method. Cinnamon oil nanoemulsion exhibited a more vigorous DPPH radical scavenging activity (IC50 = 1.81 mg/L) than the standard α-tocopherol (IC50 = 9.16 mg/L). Cinnamaldehyde nanoemulsion exhibited the highest antibacterial action against Salmonella typhimurium and Staphylococcus aureus (MIC = 175 and 150 mg/L, respectively). It was also displayed the highest inhibition against Candida albicans (EC50 = 19.92 mg/L). Cinnamaldehyde nanoemulsion was the most active against mycelial growth of Aspergillus flavus and A. niger (EC50 = 38.20 and 134.80 mg/L, respectively). It also presented the highest activity against spore germination. This study describes antimicrobials and antioxidants based on nanoemulsions made from natural products that can be used to treat microbial infections.
摘要将肉桂精油及其主要成分肉桂醛加入到纳米乳液中,以提高纳米乳液的稳定性和活性。采用高能超声法制备了水包油纳米乳。肉桂油纳米乳对DPPH自由基的清除率(IC50 = 1.81 mg/L)高于标准α-生育酚(IC50 = 9.16 mg/L)。肉桂醛纳米乳对鼠伤寒沙门菌和金黄色葡萄球菌的抑菌作用最高(MIC分别为175和150 mg/L)。对白色念珠菌的抑制作用最高(EC50 = 19.92 mg/L)。肉桂醛纳米乳对黄曲霉和黑曲霉菌丝生长的抑制作用最强(EC50分别为38.20和134.80 mg/L)。抗孢子萌发活性最高。这项研究描述了基于天然产物制成的纳米乳液的抗菌剂和抗氧化剂,可用于治疗微生物感染。
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引用次数: 8
Seasonal variation in the essential oil of Satureja kitaibelii determines chemotypes 木树精油的季节变化决定了其化学型
IF 3 3区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2022-07-28 DOI: 10.1080/10412905.2022.2103596
D. Miladinović, M. Dimitrijević, L. C. Miladinović, M. Marković, G. Stojanović
ABSTRACT The aim of the present study was a chemical and chemometric analysis of Satureja kitaibelii Wierzb. ex Heuff. essential oil during different stages of development, with emphasis on chemotaxonomy. The most abundant compound class in the S. kitaibelii oil during examined phenological stages was oxygenated monoterpenes (21.3–61.2%), except vegetative stage, in which monoterpene hydrocarbons are dominant (51.1%). Geraniol, limonene, p-cymene and borneol are the most abundant compounds of essential oil during different stages of development. The highest variation in content, during plant development, was shown by the most dominant component, geraniol (coefficient of variation 112.3%). Chemometric analysis indicated two chemotypes: limonene/p-cymene/geraniol and limonene/p-cymene/borneol. We are of the opinion that the three most abundant EO components should determine the chemotype. Our results lead to the conclusion that essential oil isolated from the plant in 1 month should not be used to determine the chemotype.
摘要:本研究的目的是对木参的化学和化学计量学进行分析。Heuff交货。精油在不同发展阶段,重点是化学分类学。在各物候阶段,除营养期以单萜烃为主(51.1%)外,油中含氧单萜烯含量最高(21.3 ~ 61.2%)。香叶醇、柠檬烯、对花香烃和冰片是不同发育阶段香精油中含量最多的化合物。在植株发育过程中,香叶醇含量变化最大,变异系数为112.3%。化学计量学分析显示两种化学型:柠檬烯/对伞花素/香叶醇和柠檬烯/对伞花素/冰片。我们认为三种最丰富的EO成分应该决定化学型。我们的结果得出结论,从该植物中分离1个月的精油不应该用于确定化学型。
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引用次数: 3
Beneficial effects of Achillea millefolium on skin injuries; a literature review 阿喀琉叶对皮肤损伤的有益作用文献综述
IF 3 3区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2022-07-28 DOI: 10.1080/10412905.2022.2104392
A. Dalili, Soheila Ebrahimnia Milani, N. Kamali, Sara Mohammadi, M. Pakbaz, S. Jamalnia, M. Sadeghi
ABSTRACT Various plants and natural compounds have been used traditionally to treat and prevent diseases, relieve pain, increase body strength, and heal skin injury. Achillea millefolium is a well-known plant of the Asteraceae family that has been extensively studied for its therapeutic properties in conditions, such as menstrual disorders, malaria, headache, cough, and skin injury. Several studies have shown that the topical application of different types of A. millefolium extracts (essential oil (EO), hydroalcoholic extract, etc.) improves the healing process of various skin injuries. Previous studies also highlighted the antibacterial, anti-inflammatory, antioxidant, and moisturizing effects of the A. millefolium extract on ameliorating skin injuries, caused by the presence of various potent constituents in the plant extract. This review aimed at overviewing the studies, focusing on A. millefolium’s possible effects on the different wound injuries.
传统上,各种植物和天然化合物被用来治疗和预防疾病、缓解疼痛、增强身体力量和治愈皮肤损伤。千叶阿喀琉斯是一种著名的菊科植物,因其治疗月经紊乱、疟疾、头痛、咳嗽和皮肤损伤等疾病的特性而被广泛研究。几项研究表明,局部应用不同类型的千叶提取物(精油(EO),水醇提取物等)可以改善各种皮肤损伤的愈合过程。以前的研究也强调了千叶提取物的抗菌、抗炎、抗氧化和保湿作用,可以改善皮肤损伤,这是由植物提取物中各种有效成分的存在引起的。本综述旨在对研究进行综述,重点研究千叶草对不同伤口损伤的可能影响。
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引用次数: 4
Agronomic traits and essential oil profiles of Humulus lupulus L. cultivated in southern Italy 意大利南部栽培葎草的农艺性状及挥发油谱
IF 3 3区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2022-07-25 DOI: 10.1080/10412905.2022.2103190
F. Gresta, Antonio Calvi, C. Santonoceto, T. Strano, G. Ruberto
ABSTRACT Humulus lupulus L. is a dioecious herbaceous perennial plant with a climbing habit, whose female inflorescences, commonly known as cones, produce and accumulate bitter substances and essential oils. The present study aimed to assess the adaptability of some American hop varieties (Cascade, Chinook, and Comet) in the Mediterranean environment of the Calabria region (Italy) through the evaluation of the morpho-biological and productive characteristics and the characterization of the aromatic traits of the inflorescences. Comet proved to be the most productive variety, while Chinook the earliest. Essential oils obtained by hydrdistillation were analysed by GC-FID and GC-MS. Myrcene, β-caryphyllene and α-humulene were the main components. The combination of morphological traits and the aromatic profiles could be adopted as tools to discriminate varieties. Comet was the most productive variety, while Chinook emerged as the earliest one. Concernng the essential oils Cascade and Comet showed similar profiles, Chinnok was different especially for the sesquiterpenes content.
葎草(Humulus lupulus L.)是一种多年生雌雄异株草本植物,具有攀缘性,其雌花花序俗称球果,产生并积累苦味物质和精油。本研究旨在通过对美洲一些啤酒花品种(Cascade、Chinook和Comet)的形态生物学和生产特性的评价以及花序芳香性状的表征,来评估它们对意大利卡拉布里亚地区地中海环境的适应性。彗星被证明是产量最高的品种,而奇努克是最早的品种。采用气相色谱- fid和气相色谱-质谱法对加氢蒸馏得到的精油进行分析。月桂烯、β-石竹烯和α-葎草烯为主要成分。形态性状与芳香性状的结合可以作为品种鉴别的工具。彗星是产量最高的品种,而奇努克是最早出现的品种。在精油中,Cascade和Comet具有相似的特征,而Chinnok的倍半萜含量则有所不同。
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引用次数: 1
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Journal of Essential Oil Research
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