Pub Date : 2022-09-30DOI: 10.1080/10412905.2022.2115570
M. Teixeira, M. Cardoso, V. Ferreira, G. A. Campolina, A. Caetano, Cassia Duarte Oliveira, M. V. P. Alves, D. Nelson
ABSTRACT The essential oils from Cantinoa carpinifolia (Benth.) and Lippia origanoides (Kunth.) were obtained by hydrodistillation. The constituents were determined by GC-MS and GC-FID. The antioxidant activities were evaluated by the ABTS, DPPH and hydroxyl radicals, phosphomolybdenum complex, reducing power, β-carotene/linoleic acid and the TBARS methods. Analysis of variance and the Scott–Knott test of the means at 5% were performed. The principal constituents in the essential oil from C.carpinifolia were α-thujone (32.15%) and β-thujone (22.22%), whereas carvacrol (41.51%), p-cymene (18.36%) and γ-terpinene (17.03%) were obtained from L.origanoides. The respective IC50 for the essential oils from C. carpinifolia and L.origanoides in the ABTS tests were >1000 and 110 µg mL−1; DPPH (110 and >1000 µg mL−1), hydroxyl (636 and 484 µg mL−1), β-carotene (>2000 and 215 µg mL−1) and TBARS (778 and 527µg mL−1). The L. origanoides essential oil was the most effective antioxidant.
摘要:采用加氢蒸馏法提取了山茱萸(Cantinoa carpinifolia)和鹤皮草(Lippia origanoides)的精油。采用气相色谱-质谱法和气相色谱- fid法测定其成分。采用ABTS、DPPH和羟基自由基、磷钼配合物、还原力、β-胡萝卜素/亚油酸和TBARS法评价其抗氧化活性。进行方差分析和均数在5%处的Scott-Knott检验。石竹挥发油的主要成分为α-图琼(32.15%)和β-图琼(22.22%)，石竹挥发油的主要成分为香芹酚(41.51%)、对伞花烃(18.36%)和γ-松油烯(17.03%)。在ABTS试验中，石竹挥发油和石竹挥发油的IC50分别>1000和110µg mL−1;DPPH(110和>1000µg mL - 1)，羟基(636和484µg mL - 1)， β-胡萝卜素(>2000和215µg mL - 1)和TBARS(778和527µg mL - 1)。牛油草精油的抗氧化效果最好。
{"title":"Evaluation of the antioxidant activity of the essential oils from Cantinoa carpinifolia (Benth.) and Lippia origanoides (Kunth.) by various methods","authors":"M. Teixeira, M. Cardoso, V. Ferreira, G. A. Campolina, A. Caetano, Cassia Duarte Oliveira, M. V. P. Alves, D. Nelson","doi":"10.1080/10412905.2022.2115570","DOIUrl":"https://doi.org/10.1080/10412905.2022.2115570","url":null,"abstract":"ABSTRACT The essential oils from Cantinoa carpinifolia (Benth.) and Lippia origanoides (Kunth.) were obtained by hydrodistillation. The constituents were determined by GC-MS and GC-FID. The antioxidant activities were evaluated by the ABTS, DPPH and hydroxyl radicals, phosphomolybdenum complex, reducing power, β-carotene/linoleic acid and the TBARS methods. Analysis of variance and the Scott–Knott test of the means at 5% were performed. The principal constituents in the essential oil from C.carpinifolia were α-thujone (32.15%) and β-thujone (22.22%), whereas carvacrol (41.51%), p-cymene (18.36%) and γ-terpinene (17.03%) were obtained from L.origanoides. The respective IC50 for the essential oils from C. carpinifolia and L.origanoides in the ABTS tests were >1000 and 110 µg mL−1; DPPH (110 and >1000 µg mL−1), hydroxyl (636 and 484 µg mL−1), β-carotene (>2000 and 215 µg mL−1) and TBARS (778 and 527µg mL−1). The L. origanoides essential oil was the most effective antioxidant.","PeriodicalId":15782,"journal":{"name":"Journal of Essential Oil Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74406312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-30DOI: 10.1080/10412905.2022.2115568
R. Vargas, Graciane Fabiela da Silva, A. Lucas, Caroline Garcia Finkler da Silva, L. Jank, F. Barreto, E. Cassel
ABSTRACT This work aimed to obtain and investigate the properties and composition of extracts from flowers of Acacia mearnsii; this tree is widely cultivated because the bark is used as a raw material for various industry segments, but the flowers are not yet explored. The essential oil was evaluated by GC/FID, GC/MS, and GC/O. The antioxidant activity (DPPH and phosphomolybdenum methods) and chemical composition were investigated for a water-soluble extract, a by-product of the extraction process. 85.8% of the compounds present in the essential oil were identified, 8-heptadecene being the major compound. The main scents detected by the olfactory analysis were roast nutty, coffee, sweet, and floral. The water-soluble extract showed antioxidant activity that may be related to phenolic compounds. Results obtained from the LC-qTOF/MS analysis have shown that 25 likely flavonoids, such as naringenin, myricetin, quercetin, and rutin are present in the extract.
{"title":"Investigation of essential oil and water-soluble extract obtained by steam distillation from Acacia mearnsii flowers","authors":"R. Vargas, Graciane Fabiela da Silva, A. Lucas, Caroline Garcia Finkler da Silva, L. Jank, F. Barreto, E. Cassel","doi":"10.1080/10412905.2022.2115568","DOIUrl":"https://doi.org/10.1080/10412905.2022.2115568","url":null,"abstract":"ABSTRACT This work aimed to obtain and investigate the properties and composition of extracts from flowers of Acacia mearnsii; this tree is widely cultivated because the bark is used as a raw material for various industry segments, but the flowers are not yet explored. The essential oil was evaluated by GC/FID, GC/MS, and GC/O. The antioxidant activity (DPPH and phosphomolybdenum methods) and chemical composition were investigated for a water-soluble extract, a by-product of the extraction process. 85.8% of the compounds present in the essential oil were identified, 8-heptadecene being the major compound. The main scents detected by the olfactory analysis were roast nutty, coffee, sweet, and floral. The water-soluble extract showed antioxidant activity that may be related to phenolic compounds. Results obtained from the LC-qTOF/MS analysis have shown that 25 likely flavonoids, such as naringenin, myricetin, quercetin, and rutin are present in the extract.","PeriodicalId":15782,"journal":{"name":"Journal of Essential Oil Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82302013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-20DOI: 10.1080/10412905.2022.2118880
Valeria Reyes-Pérez, V. Granados-Soto, E. Linares, R. Bye, R. Mata, M. Déciga-Campos
ABSTRACT Zinnia grandiflora (Asteraceae) is used to alleviate several painful disorders. The effects of an essential oil prepared by hydrodistillation from Z. grandiflora (EOZ) and its major compound on neuropathic pain, allodynia, and inflammation in mice were assessed. Headspace-solid phase microextraction analysis of an aqueous extract was also carried out. GC-MS analyses of all volatiles revealed that germacrene D (13.24%) and desmethylisoencecalin (DME) (13.24%) were the most abundant compounds. EOZ was effective in reducing inflammation and allodynia in mice. EOZ (300 mg/paw) reverted tactile allodynia more effectively than gabapentin (100 μg/paw). DME (0.1‒10 µg/paw) responses in the carrageenan model were concentration-dependent; the maximum effect was observed at 10 µg/paw, like that of diclofenac (30 μg/paw). In the spinal nerve ligation model, DME (0.1‒10 μg/paw) exerted a concentration-dependent anti-allodynic action; its effect was better than that of gabapentin. A method to quantify zinagrandinolide E in the crude drug was developed and validated.
{"title":"Anti-inflammatory and anti-allodynic activities in mice of the essential oil and desmethylisoencecalin from Zinnia grandiflora Nuttall","authors":"Valeria Reyes-Pérez, V. Granados-Soto, E. Linares, R. Bye, R. Mata, M. Déciga-Campos","doi":"10.1080/10412905.2022.2118880","DOIUrl":"https://doi.org/10.1080/10412905.2022.2118880","url":null,"abstract":"ABSTRACT Zinnia grandiflora (Asteraceae) is used to alleviate several painful disorders. The effects of an essential oil prepared by hydrodistillation from Z. grandiflora (EOZ) and its major compound on neuropathic pain, allodynia, and inflammation in mice were assessed. Headspace-solid phase microextraction analysis of an aqueous extract was also carried out. GC-MS analyses of all volatiles revealed that germacrene D (13.24%) and desmethylisoencecalin (DME) (13.24%) were the most abundant compounds. EOZ was effective in reducing inflammation and allodynia in mice. EOZ (300 mg/paw) reverted tactile allodynia more effectively than gabapentin (100 μg/paw). DME (0.1‒10 µg/paw) responses in the carrageenan model were concentration-dependent; the maximum effect was observed at 10 µg/paw, like that of diclofenac (30 μg/paw). In the spinal nerve ligation model, DME (0.1‒10 μg/paw) exerted a concentration-dependent anti-allodynic action; its effect was better than that of gabapentin. A method to quantify zinagrandinolide E in the crude drug was developed and validated.","PeriodicalId":15782,"journal":{"name":"Journal of Essential Oil Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88581804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-07DOI: 10.1080/10412905.2022.2118879
Yi-Xi Feng, Xin-Xin Lu, Yue Du, Jia-Wei Zhang, Borjigidai Almaz, Ding Zeng, S. Du
ABSTRACT In this work, the essential oils (EOs) from leaves and flowers of Rhododendron spinuliferum and Rhododendron spiciferum were extracted by hydrodistillation, chemical compositions of EOs were analyzed by GC-MS and GC-FID. For R. spinuliferum and R. spiciferum, α- and β-pinene were common major compounds. It provided an opportunity to test the potential synergistic effect of α- and β-pinene among EOs. Firstly, four EOs and two major compounds (α- and β-pinene) exhibited fumigant, contact and repellent activities to three stored-product insects. To the red flour beetle, leaves of both species exhibited stronger contact toxicity than flowers did. This trend was diverse to the booklouse. Furthermore, binary mixture bioassays of α- and β-pinene were performed following natural proportions. Results of multiple linear regression showed that α-pinene, trans-β-farnesene and bornyl acetate were responsible for the contact toxicity to the red flour beetle and booklouse with diverse contribution.
{"title":"Synergized potential, insecticidal and repellent activity of essential oils from two Rhododendron species against three stored product insects","authors":"Yi-Xi Feng, Xin-Xin Lu, Yue Du, Jia-Wei Zhang, Borjigidai Almaz, Ding Zeng, S. Du","doi":"10.1080/10412905.2022.2118879","DOIUrl":"https://doi.org/10.1080/10412905.2022.2118879","url":null,"abstract":"ABSTRACT In this work, the essential oils (EOs) from leaves and flowers of Rhododendron spinuliferum and Rhododendron spiciferum were extracted by hydrodistillation, chemical compositions of EOs were analyzed by GC-MS and GC-FID. For R. spinuliferum and R. spiciferum, α- and β-pinene were common major compounds. It provided an opportunity to test the potential synergistic effect of α- and β-pinene among EOs. Firstly, four EOs and two major compounds (α- and β-pinene) exhibited fumigant, contact and repellent activities to three stored-product insects. To the red flour beetle, leaves of both species exhibited stronger contact toxicity than flowers did. This trend was diverse to the booklouse. Furthermore, binary mixture bioassays of α- and β-pinene were performed following natural proportions. Results of multiple linear regression showed that α-pinene, trans-β-farnesene and bornyl acetate were responsible for the contact toxicity to the red flour beetle and booklouse with diverse contribution.","PeriodicalId":15782,"journal":{"name":"Journal of Essential Oil Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84690266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-05DOI: 10.1080/10412905.2022.2118878
A. Alqudah, R. AbuDalo, E. Qnais, Mohammaed Wedyan, Taima Qudah, Muna Oqal
ABSTRACT The aims of this study were to evaluate the anti-inflammatory activity and the possible anti-inflammatory mechanisms of Anastatica hierochuntica essential oil (EOAH). The essential oils were obtained by hydrodistillation of the aerial parts of the plant, and analyzed using gas chromatography and mass spectrometry (GC/MS). Their anti-inflammatory activity was evaluated using classical model of inflammation (carrageenan-induced paw edema and peritonitis). Nitric oxide radical-scavenging activity and lipid peroxidation were determined to assess the antioxidant activity of the essential oil. The EOAH mainly comprised Cuminaldehyde (33.8%), trans-β-Caryophyllene (32.5%), and Linalool (8.2%). EOAH significantly reduced edema, peritonitis, myeloperoxidase activity, and NOx-peritoneal lavage concentration induced by carrageenan. In addition, nitric oxide radical generated from sodium nitroprusside was significantly inhibited by the essential oil. Furthermore, EOAH was able to prevent Fe+2- or Fe+2 plus H2O2-induced lipid peroxidation. In conclusion, the EOAH exerts anti-inflammatory activity that could be related to its antioxidant capacity.
{"title":"Potential anti-inflammatory activity of the Anastatica hierochuntica essential oil","authors":"A. Alqudah, R. AbuDalo, E. Qnais, Mohammaed Wedyan, Taima Qudah, Muna Oqal","doi":"10.1080/10412905.2022.2118878","DOIUrl":"https://doi.org/10.1080/10412905.2022.2118878","url":null,"abstract":"ABSTRACT The aims of this study were to evaluate the anti-inflammatory activity and the possible anti-inflammatory mechanisms of Anastatica hierochuntica essential oil (EOAH). The essential oils were obtained by hydrodistillation of the aerial parts of the plant, and analyzed using gas chromatography and mass spectrometry (GC/MS). Their anti-inflammatory activity was evaluated using classical model of inflammation (carrageenan-induced paw edema and peritonitis). Nitric oxide radical-scavenging activity and lipid peroxidation were determined to assess the antioxidant activity of the essential oil. The EOAH mainly comprised Cuminaldehyde (33.8%), trans-β-Caryophyllene (32.5%), and Linalool (8.2%). EOAH significantly reduced edema, peritonitis, myeloperoxidase activity, and NOx-peritoneal lavage concentration induced by carrageenan. In addition, nitric oxide radical generated from sodium nitroprusside was significantly inhibited by the essential oil. Furthermore, EOAH was able to prevent Fe+2- or Fe+2 plus H2O2-induced lipid peroxidation. In conclusion, the EOAH exerts anti-inflammatory activity that could be related to its antioxidant capacity.","PeriodicalId":15782,"journal":{"name":"Journal of Essential Oil Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87544895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-29DOI: 10.1080/10412905.2021.1937354
Leoncio Solís-Quispe, J. Pino, Jessica Z. Marín-Villa, Ciro Tomaylla-Cruz, Jorge A. Solís-Quispe, Luz J. Aragón-Alencastre, Ivones Hernández, Cindel Cuellar, I. Rodeiro, M. Fernández
ABSTRACT The chemical composition and antioxidant activity of the essential oil from the leaves of Ambrosia arborescens Miller grown in the Peruvian Andes were studied. One hundred and thirteen compounds were identified, which represents 94.7% of the total composition of the essential oil. Sesquiterpene hydrocarbons and oxygenated monoterpenes were the most represented classes of volatiles with 43.8% and 22.4%, respectively. The main constituents of the essential oil were β-acoradiene (15.3%) and chrysanthenone (11.3%). Antioxidant activities were examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and determination of lipid peroxidation in brain rat homogenates (LPO) assays. The essential oil had a free radical scavenging effect, and it displayed ferric reducing capacity and antioxidant activity by inhibition of LPO.
{"title":"Chemical composition and antioxidant activity of Ambrosia arborescens Miller leaf essential oil from Peruvian Andes","authors":"Leoncio Solís-Quispe, J. Pino, Jessica Z. Marín-Villa, Ciro Tomaylla-Cruz, Jorge A. Solís-Quispe, Luz J. Aragón-Alencastre, Ivones Hernández, Cindel Cuellar, I. Rodeiro, M. Fernández","doi":"10.1080/10412905.2021.1937354","DOIUrl":"https://doi.org/10.1080/10412905.2021.1937354","url":null,"abstract":"ABSTRACT The chemical composition and antioxidant activity of the essential oil from the leaves of Ambrosia arborescens Miller grown in the Peruvian Andes were studied. One hundred and thirteen compounds were identified, which represents 94.7% of the total composition of the essential oil. Sesquiterpene hydrocarbons and oxygenated monoterpenes were the most represented classes of volatiles with 43.8% and 22.4%, respectively. The main constituents of the essential oil were β-acoradiene (15.3%) and chrysanthenone (11.3%). Antioxidant activities were examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and determination of lipid peroxidation in brain rat homogenates (LPO) assays. The essential oil had a free radical scavenging effect, and it displayed ferric reducing capacity and antioxidant activity by inhibition of LPO.","PeriodicalId":15782,"journal":{"name":"Journal of Essential Oil Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86890308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-19DOI: 10.1080/10412905.2022.2103043
Erika da Silva Monteiro, Kelly de Sousa Monteiro, Patrícia da Silva Montes, Cláudio Augusto Gomes Camara, M. M. Moraes, C. Fagg, Daniel Oliveira Freire, Eliana Fortes Gris, I. C. Rodrigues da Silva, L. C. Sá-Barreto, Daniela Castilho Orsi
ABSTRACT This study aimed to evaluate the chemical and antibacterial properties of the essential oil of B. dracunculifolia from three regions of Brazil: Midwest, Southeast and South region. The major compounds of essential oil from Midwest region were (E)-nerolidol (28.15%) and spathulenol (17.68%), both oxygenated sesquiterpenes. And the major compounds of essential oils from Southeast and South regions were (E)-nerolidol (12.75–13.95%), bicyclogermacrene (10.76–14.21%) and limonene (10.49–19.36%). The three essential oils showed antibacterial activity for both Gram-positive and Gram-negative bacteria tested, with MIC values from 0.03 to 0.15 mg/mL and MBC values from 0.05 to 0.30 mg/mL. The B. dracunculifolia essential oil from the Midwest region of Brazil presented higher values of oxygenated sesquiterpenes and was more effective on all tested bacterial strains, with lower and statistically significant MBC values, compared to the oils from the Southeast and South regions of Brazil
{"title":"Chemical and antibacterial properties of Baccharis dracunculifolia DC essential oils from different regions of Brazil","authors":"Erika da Silva Monteiro, Kelly de Sousa Monteiro, Patrícia da Silva Montes, Cláudio Augusto Gomes Camara, M. M. Moraes, C. Fagg, Daniel Oliveira Freire, Eliana Fortes Gris, I. C. Rodrigues da Silva, L. C. Sá-Barreto, Daniela Castilho Orsi","doi":"10.1080/10412905.2022.2103043","DOIUrl":"https://doi.org/10.1080/10412905.2022.2103043","url":null,"abstract":"ABSTRACT This study aimed to evaluate the chemical and antibacterial properties of the essential oil of B. dracunculifolia from three regions of Brazil: Midwest, Southeast and South region. The major compounds of essential oil from Midwest region were (E)-nerolidol (28.15%) and spathulenol (17.68%), both oxygenated sesquiterpenes. And the major compounds of essential oils from Southeast and South regions were (E)-nerolidol (12.75–13.95%), bicyclogermacrene (10.76–14.21%) and limonene (10.49–19.36%). The three essential oils showed antibacterial activity for both Gram-positive and Gram-negative bacteria tested, with MIC values from 0.03 to 0.15 mg/mL and MBC values from 0.05 to 0.30 mg/mL. The B. dracunculifolia essential oil from the Midwest region of Brazil presented higher values of oxygenated sesquiterpenes and was more effective on all tested bacterial strains, with lower and statistically significant MBC values, compared to the oils from the Southeast and South regions of Brazil","PeriodicalId":15782,"journal":{"name":"Journal of Essential Oil Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89069702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-13DOI: 10.1080/10412905.2022.2103191
J. Llorens-Molina, S. Vacas, Natalia Escrivá, M. Verdeguer
ABSTRACT A wild population of Thymus piperella L. was monitored for two years in order to correlate phenological stages and meteorological data with yield and, essential oil (EO) composition. The plant material was extracted by hydrodistillation and the EO was submitted to GC-MS and GC-FID analysis. To achieve reliable quantification, relative response factors were applied. The results showed a progressive increase in the proportion of carvacrol according to the sunlight duration and temperature increase, until reaching a maximum at the beginning of flowering stage (52.9 % and 41.0 % in 2018 and 2019, respectively). A simultaneous decrease was found in the amount of p-cymene (8.2 % and 12.8 % respectively) in the same stage, whereas -terpinene showed a similar evolution to that observed for carvacrol but with lower values (3.3 % - 14.6 %). From statistical analysis, significant changes in the relative proportions of carvacrol and its metabolic precursors: γ-terpinene and p-cymene were found. They have been explained based on the proposed metabolic pathways in the literature. On the other hand, when relating the changes in the EO profiles with rainfall data, a strong influence of the water supply on the evolution of the chemical composition was observed.
{"title":"Seasonal variation of Thymus piperella L. essential oil composition. Relationship among γ-terpinene, p-cymene and carvacrol","authors":"J. Llorens-Molina, S. Vacas, Natalia Escrivá, M. Verdeguer","doi":"10.1080/10412905.2022.2103191","DOIUrl":"https://doi.org/10.1080/10412905.2022.2103191","url":null,"abstract":"ABSTRACT A wild population of Thymus piperella L. was monitored for two years in order to correlate phenological stages and meteorological data with yield and, essential oil (EO) composition. The plant material was extracted by hydrodistillation and the EO was submitted to GC-MS and GC-FID analysis. To achieve reliable quantification, relative response factors were applied. The results showed a progressive increase in the proportion of carvacrol according to the sunlight duration and temperature increase, until reaching a maximum at the beginning of flowering stage (52.9 % and 41.0 % in 2018 and 2019, respectively). A simultaneous decrease was found in the amount of p-cymene (8.2 % and 12.8 % respectively) in the same stage, whereas -terpinene showed a similar evolution to that observed for carvacrol but with lower values (3.3 % - 14.6 %). From statistical analysis, significant changes in the relative proportions of carvacrol and its metabolic precursors: γ-terpinene and p-cymene were found. They have been explained based on the proposed metabolic pathways in the literature. On the other hand, when relating the changes in the EO profiles with rainfall data, a strong influence of the water supply on the evolution of the chemical composition was observed.","PeriodicalId":15782,"journal":{"name":"Journal of Essential Oil Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76258039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-11DOI: 10.1080/10412905.2022.2107101
E. Becer, E. M. Altundag, K. Başer, H. Vatansever, M. Bayar
ABSTRACT Origanum onites L. (O. onites) is one of the cultivated Origanum species in Turkey. The aim of this study was to determine the cytotoxicity and cellular antioxidant effects of O. onites essential oil, carvacrol and p-cymene on HCT116 and HepG2 cells. O. onites essential oil components were analyzed by GC/MS and GC/FID. Cytotoxicity was analyzed via the MTT assay in HCT116 and HepG2 cells. Cellular antioxidant activities of O. onites essential oil, carvacrol and p-cymene were determined with DCFH-DA method. GC/MS analysis identified carvacrol (%78.4) as the main constituent of O. onites essential oil. 400 µg/ml concentration of O. onites essential oil, carvacrol and p-cymene were found to be active against HCT116 and HepG2 cells. Also, carvacrol showed higher cellular antioxidant activity than O. onites essential oil and p-cymene. We conclude that O. onites essential oil and carvacrol may be a candidate for pharmaceutical products in treatment of cancer.
土一枝(Origanum onites L.， O. onites)是土耳其的一种人工培育的土一枝。本研究的目的是研究石杉精油、香芹酚和对花香烃对HCT116和HepG2细胞的细胞毒性和细胞抗氧化作用。采用GC/MS、GC/FID等分析方法对黄芪挥发油成分进行了分析。MTT法检测HCT116和HepG2细胞的细胞毒性。采用DCFH-DA法测定了龙葵挥发油、香芹酚和对花香烃的细胞抗氧化活性。GC/MS分析鉴定出香芹酚(% 78.4%)为龙葵挥发油的主要成分。400µg/ml浓度的龙葵精油、香芹酚和对花香烃对HCT116和HepG2细胞均有抑制作用。此外，香芹酚的细胞抗氧化活性也高于石楠精油和对花香烃。我们得出结论，龙葵精油和香芹酚可能是治疗癌症的候选药物产品。
{"title":"Cytotoxic activity and antioxidant effects of Origanum onites essential oil and its two major contents, carvacrol and p-cymene on human colorectal (HCT116) and hepatocelluler carcinoma (HepG2) cell lines","authors":"E. Becer, E. M. Altundag, K. Başer, H. Vatansever, M. Bayar","doi":"10.1080/10412905.2022.2107101","DOIUrl":"https://doi.org/10.1080/10412905.2022.2107101","url":null,"abstract":"ABSTRACT Origanum onites L. (O. onites) is one of the cultivated Origanum species in Turkey. The aim of this study was to determine the cytotoxicity and cellular antioxidant effects of O. onites essential oil, carvacrol and p-cymene on HCT116 and HepG2 cells. O. onites essential oil components were analyzed by GC/MS and GC/FID. Cytotoxicity was analyzed via the MTT assay in HCT116 and HepG2 cells. Cellular antioxidant activities of O. onites essential oil, carvacrol and p-cymene were determined with DCFH-DA method. GC/MS analysis identified carvacrol (%78.4) as the main constituent of O. onites essential oil. 400 µg/ml concentration of O. onites essential oil, carvacrol and p-cymene were found to be active against HCT116 and HepG2 cells. Also, carvacrol showed higher cellular antioxidant activity than O. onites essential oil and p-cymene. We conclude that O. onites essential oil and carvacrol may be a candidate for pharmaceutical products in treatment of cancer.","PeriodicalId":15782,"journal":{"name":"Journal of Essential Oil Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74529107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-08DOI: 10.1080/10412905.2022.2109767
L. Oucheikh, Omar Ou-ani, Soumia Moujane, A. Ansari, A. Oubair, M. Znini
ABSTRACT This study describes the chemical composition and the in vitro antifungal activity of the essential oil (EO) of Anvillea gracinii subsp. radiata aerial parts against three phytopathogenic using the poisoned food (PF) and the volatile assay (VA) techniques. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS) allowed the identification of 35 compounds which cis 8-acetoxychrysantenyl acetate (cis8ACA) (30.8%), cis-chrysanthenyl acetate (15.5%), 6-oxocyclonerolidol (14.9%) and τ-cadinol (7.4%) are the main constituents. In VA test, the minimum inhibitory concentrations (MIC) are 0.50 µL/mL for B. cinerea and P. expansum and 1.00 µL/mL for R. stolonifer, but they are 0.125 µL/mL for B. cinerea and 0.25 µL/mL for other fungi using PF technique. Molecular docking and molecular dynamics (MD) simulations revealed that cis8ACA has the smallest docking energy (-8.9 kcal.mol-1) indicating its high affinity to bind to the active site of the C14DM thereby forming a stable cis8ACA -C14DM complex.
{"title":"Chemical composition, in vitro antifungal activity, DFT, molecular docking and molecular dynamics simulation studies of the essential oil from Anvillea gracinii subsp. radiata (Coss. & Durieu) Anderb","authors":"L. Oucheikh, Omar Ou-ani, Soumia Moujane, A. Ansari, A. Oubair, M. Znini","doi":"10.1080/10412905.2022.2109767","DOIUrl":"https://doi.org/10.1080/10412905.2022.2109767","url":null,"abstract":"ABSTRACT This study describes the chemical composition and the in vitro antifungal activity of the essential oil (EO) of Anvillea gracinii subsp. radiata aerial parts against three phytopathogenic using the poisoned food (PF) and the volatile assay (VA) techniques. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS) allowed the identification of 35 compounds which cis 8-acetoxychrysantenyl acetate (cis8ACA) (30.8%), cis-chrysanthenyl acetate (15.5%), 6-oxocyclonerolidol (14.9%) and τ-cadinol (7.4%) are the main constituents. In VA test, the minimum inhibitory concentrations (MIC) are 0.50 µL/mL for B. cinerea and P. expansum and 1.00 µL/mL for R. stolonifer, but they are 0.125 µL/mL for B. cinerea and 0.25 µL/mL for other fungi using PF technique. Molecular docking and molecular dynamics (MD) simulations revealed that cis8ACA has the smallest docking energy (-8.9 kcal.mol-1) indicating its high affinity to bind to the active site of the C14DM thereby forming a stable cis8ACA -C14DM complex.","PeriodicalId":15782,"journal":{"name":"Journal of Essential Oil Research","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79715992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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