I. Elkoraichi, Mounia Latif, Othman El Faqer, Rajaa Elaje, A. Thoume, E. Mtairag, S. Rais, F. Daoudi
Introduction: The use of pomegranates in Moroccan pharmacopeia is due to their healing and nutritional properties because of their richness in secondary molecules. The following study analyses the composition of the aqueous extract of Punica granatum peel and evaluates in vivo and in vitro antioxidant effects, hemolytic protection, and acute toxicity. Methods: Quantification of the plant extract was realized by high-performance liquid chromatography (HPLC). The hemolytic assay was used for erythrocyte protection, while the in vitro antioxidant effect was evaluated by 2, 20-azinobis-(3-ethylbenzothiazoneline-6-sulphonic acid) (ABTS) and reducing ferric power (RFP) assays. The in-vivo antioxidant activity was tested by measuring levels of lipid peroxidation (LPO) in serum. The toxicological study was tested by oral administration of the extract to four groups of mice for 21 days, followed by a histopathological examination of the spleen. Results: HPLC analysis showed the presence of some phenolic compounds such as coumarin, caffeic, gallic and syringic acids. The IC50 of the antioxidant assays were 254.49 ± 62.17 μg/mL and 40.265 ± 2.9 μg/mL for ABTS and reducing power, respectively. Furthermore, the thiobarbituric acid reactive substances (TBARS) assay showed the lowest levels at 150 mg/mL concentration. All of the concentrations used for hemolytic protection did not exceed 15% of hemolysis. Moreover, the toxicity test showed no sign of mortality, signs of weakness, or weight loss; also the histopathological examination of the spleen tissues showed the absence of any damage. Conclusion: The peel extract of P. granatum showed good potential and could be exploited as a natural antioxidant and antihemolytic remedy, leading to the development of new drugs.
{"title":"In vivo and in vitro evaluation of antioxidant, antihemolytic, acute toxicity effects and high-performance liquid chromatography analysis of an aqueous extract of Moroccan Punica granatum L.","authors":"I. Elkoraichi, Mounia Latif, Othman El Faqer, Rajaa Elaje, A. Thoume, E. Mtairag, S. Rais, F. Daoudi","doi":"10.34172/jhp.2023.17","DOIUrl":"https://doi.org/10.34172/jhp.2023.17","url":null,"abstract":"Introduction: The use of pomegranates in Moroccan pharmacopeia is due to their healing and nutritional properties because of their richness in secondary molecules. The following study analyses the composition of the aqueous extract of Punica granatum peel and evaluates in vivo and in vitro antioxidant effects, hemolytic protection, and acute toxicity. Methods: Quantification of the plant extract was realized by high-performance liquid chromatography (HPLC). The hemolytic assay was used for erythrocyte protection, while the in vitro antioxidant effect was evaluated by 2, 20-azinobis-(3-ethylbenzothiazoneline-6-sulphonic acid) (ABTS) and reducing ferric power (RFP) assays. The in-vivo antioxidant activity was tested by measuring levels of lipid peroxidation (LPO) in serum. The toxicological study was tested by oral administration of the extract to four groups of mice for 21 days, followed by a histopathological examination of the spleen. Results: HPLC analysis showed the presence of some phenolic compounds such as coumarin, caffeic, gallic and syringic acids. The IC50 of the antioxidant assays were 254.49 ± 62.17 μg/mL and 40.265 ± 2.9 μg/mL for ABTS and reducing power, respectively. Furthermore, the thiobarbituric acid reactive substances (TBARS) assay showed the lowest levels at 150 mg/mL concentration. All of the concentrations used for hemolytic protection did not exceed 15% of hemolysis. Moreover, the toxicity test showed no sign of mortality, signs of weakness, or weight loss; also the histopathological examination of the spleen tissues showed the absence of any damage. Conclusion: The peel extract of P. granatum showed good potential and could be exploited as a natural antioxidant and antihemolytic remedy, leading to the development of new drugs.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49206244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sunil Kumar, K. Ratha, Meda Mruthyumjaya Rao, R. Acharya
Since ancient times, people have used medicinal plants as a source of medications to treat and prevent diseases. Paeonia species are important therapeutic plants in Ayurvedic, Unani, and Traditional Chinese Medicine. This study aims to provide updated information on the ethnobotany, phytochemistry, and pharmacological activities of Paeonia species discovered until now. Using the keywords "Paeonia", "geographical distribution", "ethnopharmacology and traditional values", "phytochemistry", "antioxidant", "anti-inflammatory", "antimicrobial", "cardiovascular diseases", and "anticancerous properties", the published reports from 2001 to 2022 were retrieved using Google Scholar, Science Direct, PubMed, and Scopus databases. A total of 156 published articles were studied after meeting the qualifying criteria. Out of these, 52 articles were studied for phytochemistry, ethnopharmacological and traditional uses. Paeonia emodi is used to treat hypertension, asthma, convulsions, epilepsy, bronchitis, ascites, uterine abnormalities, and a variety of skin ailments. Bioactive compounds like triterpenes, monoterpene glucosides, phenols, tannins, emodinol, benzoic acid, paeonin A and B, steroids, several secondary metabolites like paeoniflorin and paeonol, and several minerals are abundant in the Paeonia species. In recent studies, Paeonia emodi has been shown to possess pharmacological properties like antioxidant, antibacterial, anti-inflammatory, insecticidal, and anti-tumor activities. Convincing data supports the traditional ethnomedicinal claims of the plant; the abundant phytocompounds of the plant are attributed to its broad spectrum of pharmacological activities. In order to understand the molecular mechanisms underlying the action of the bioactive ingredients in drug development processes and to investigate their potential at the clinical level, more research is required.
{"title":"A comprehensive review on the phytochemistry, pharmacological, ethnobotany, and traditional uses of Paeonia species","authors":"Sunil Kumar, K. Ratha, Meda Mruthyumjaya Rao, R. Acharya","doi":"10.34172/jhp.2023.02","DOIUrl":"https://doi.org/10.34172/jhp.2023.02","url":null,"abstract":"Since ancient times, people have used medicinal plants as a source of medications to treat and prevent diseases. Paeonia species are important therapeutic plants in Ayurvedic, Unani, and Traditional Chinese Medicine. This study aims to provide updated information on the ethnobotany, phytochemistry, and pharmacological activities of Paeonia species discovered until now. Using the keywords \"Paeonia\", \"geographical distribution\", \"ethnopharmacology and traditional values\", \"phytochemistry\", \"antioxidant\", \"anti-inflammatory\", \"antimicrobial\", \"cardiovascular diseases\", and \"anticancerous properties\", the published reports from 2001 to 2022 were retrieved using Google Scholar, Science Direct, PubMed, and Scopus databases. A total of 156 published articles were studied after meeting the qualifying criteria. Out of these, 52 articles were studied for phytochemistry, ethnopharmacological and traditional uses. Paeonia emodi is used to treat hypertension, asthma, convulsions, epilepsy, bronchitis, ascites, uterine abnormalities, and a variety of skin ailments. Bioactive compounds like triterpenes, monoterpene glucosides, phenols, tannins, emodinol, benzoic acid, paeonin A and B, steroids, several secondary metabolites like paeoniflorin and paeonol, and several minerals are abundant in the Paeonia species. In recent studies, Paeonia emodi has been shown to possess pharmacological properties like antioxidant, antibacterial, anti-inflammatory, insecticidal, and anti-tumor activities. Convincing data supports the traditional ethnomedicinal claims of the plant; the abundant phytocompounds of the plant are attributed to its broad spectrum of pharmacological activities. In order to understand the molecular mechanisms underlying the action of the bioactive ingredients in drug development processes and to investigate their potential at the clinical level, more research is required.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46774983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Akankshya Sahu, G. Nayak, S. Bhuyan, Ruchi Bhuyan, D. Kar, A. Kuanar
The genus Ocimum has many species belonging to the Lamiaceae family that are used to treat various diseases and disorders. Ocimum basilicum has been found to contain over 200 chemical components in different plant parts. The bioactive components showed the presence of α-linalool, camphor, limonene, thymol, citral, β-linalool, estragole, etc. O. basilicum exhibits anti-inflammatory, antioxidant, antiulcer, antiviral, hypoglycemic, hypolipidemic, antimicrobial, anticancer, wound-healing activities, etc. They have also been used to treat fevers, digestive issues, stomach cramps, nausea, gastritis, migraine, diarrhea. This review aims to provide information about the chemical compounds and biological activities of O. basilicum var thyrsiflora (Thai basil). To the best of our knowledge, this is the first review outlining the innovative ethnomedicinal approach to oral health care that, through its strong phytoconstituents, has the potential to improve modern medicine.
{"title":"A comprehensive review on nutritive value, phytochemicals, and pharmacological activities of Ocimum basilicum var. thyrsiflora","authors":"Akankshya Sahu, G. Nayak, S. Bhuyan, Ruchi Bhuyan, D. Kar, A. Kuanar","doi":"10.34172/jhp.2023.01","DOIUrl":"https://doi.org/10.34172/jhp.2023.01","url":null,"abstract":"The genus Ocimum has many species belonging to the Lamiaceae family that are used to treat various diseases and disorders. Ocimum basilicum has been found to contain over 200 chemical components in different plant parts. The bioactive components showed the presence of α-linalool, camphor, limonene, thymol, citral, β-linalool, estragole, etc. O. basilicum exhibits anti-inflammatory, antioxidant, antiulcer, antiviral, hypoglycemic, hypolipidemic, antimicrobial, anticancer, wound-healing activities, etc. They have also been used to treat fevers, digestive issues, stomach cramps, nausea, gastritis, migraine, diarrhea. This review aims to provide information about the chemical compounds and biological activities of O. basilicum var thyrsiflora (Thai basil). To the best of our knowledge, this is the first review outlining the innovative ethnomedicinal approach to oral health care that, through its strong phytoconstituents, has the potential to improve modern medicine.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49357895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asmaa Khattab, N. Awad, Doaa A. Abdel Fadeel, M. Fadel
This review is destined for a comprehensive assessment of the phytochemistry and medicinal properties of Tecoma stans, a widely used plant in folk cultures, as a traditionally safe and effective treatment for different diseases and complications. The attainable and reachable sources of T. stans confirmed its origin, ethnopharmacological properties, and therapeutic medicinal uses. Besides a hundred chemical compounds that have been isolated, the main active constituents are flavonoids, alkaloids, phenolic acids, and fatty acids. T. stans exerted many medicinal benefits, including antidiabetic, anti-inflammatory, anti-cancer, antimicrobial, antioxidant, hepatoprotective, cardioprotective, and nephroprotective properties. However, there is a shortage of in vivo studies, especially adequate dosage and toxicity studies. More studies should be carried out for nutritional data. This review represents a scientific understanding of clinical correlations and applications of phytocompounds from T. stans in protecting and treating many complaints and disorders.
{"title":"Reviewing the reported pharmacognostic and pharmacological investigations on Tecoma stans Juss. ex Kunth","authors":"Asmaa Khattab, N. Awad, Doaa A. Abdel Fadeel, M. Fadel","doi":"10.34172/jhp.2023.03","DOIUrl":"https://doi.org/10.34172/jhp.2023.03","url":null,"abstract":"This review is destined for a comprehensive assessment of the phytochemistry and medicinal properties of Tecoma stans, a widely used plant in folk cultures, as a traditionally safe and effective treatment for different diseases and complications. The attainable and reachable sources of T. stans confirmed its origin, ethnopharmacological properties, and therapeutic medicinal uses. Besides a hundred chemical compounds that have been isolated, the main active constituents are flavonoids, alkaloids, phenolic acids, and fatty acids. T. stans exerted many medicinal benefits, including antidiabetic, anti-inflammatory, anti-cancer, antimicrobial, antioxidant, hepatoprotective, cardioprotective, and nephroprotective properties. However, there is a shortage of in vivo studies, especially adequate dosage and toxicity studies. More studies should be carried out for nutritional data. This review represents a scientific understanding of clinical correlations and applications of phytocompounds from T. stans in protecting and treating many complaints and disorders.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45946771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Secondary metabolites from plants have been found to play an important role in the treatment of diabetes mellitus (DM) and its complications. Therefore, the purpose of this study was to identify the chemical components of Annona muricata leaf chloroform fraction (CFAm) and its in vitro antioxidant properties, as well as inhibitory activity against α-amylase and α-glucosidase enzymatic activities. Methods: Gas chromatography–mass spectrometry (GC-MS) technique was engaged in the identification of phytochemical constituents. Antioxidant activities such as DPPH free radical scavenging ability, reducing power capacity, hydroxyl radical scavenging ability, singlet oxygen scavenging capacity, as well as α-amylase and α-glucosidase inhibition were carried out using standard in vitro methods. Results: GC-MS analysis of CFAm revealed the presence of 23 phytochemicals, out of which 5 compounds had the highest % compositions (i.e., octadecanoic acid (20.35%), 2-propanone, 1-(4-hydroxy-3-methoxyphenyl) (12.04%), isocomene (22.60%), 9, 12, 15 octadecatrienoic acid, methyl ester (Z,Z,Z) (28. 98%), and quercetin, 5TMS derivative (18.28%)). Also, CFAm demonstrated a significant (P<0.05) inhibition against DPPH (IC50= 26.33±1.39 mg/mL), with OH free radical scavenging capacity (65.46 ± 1.39 mg/100 g), singlet oxygen scavenging capacity (55.24 ± 1.22 mg/100 g), and showed ferric reducing power (84.52 ± 2.84 mg/100 g). Also, CFAm exhibited a significant (P<0.05) inhibition against α-glucosidase (IC50= 71.06 ±1.45 mg/ mL) and α-amylase (IC50= 73.88±1.58 mg/mL) in a concentration-dependent manners. Conclusion: The remarkable properties demonstrated by CFAm, which are essential for the management of DM, could probably be credited to the presence of the various identified phytonutrients.
{"title":"Identification of bioactive constituents of chloroform fraction from Annona muricata leaf, its antioxidant activity and inhibitory potential against carbohydrate-hydrolyzing α-amylase and α-glucosidase activities linked to type II diabetes mellitus: In vitro study","authors":"O. Olasehinde, O. B. Afolabi","doi":"10.34172/jhp.2023.09","DOIUrl":"https://doi.org/10.34172/jhp.2023.09","url":null,"abstract":"Introduction: Secondary metabolites from plants have been found to play an important role in the treatment of diabetes mellitus (DM) and its complications. Therefore, the purpose of this study was to identify the chemical components of Annona muricata leaf chloroform fraction (CFAm) and its in vitro antioxidant properties, as well as inhibitory activity against α-amylase and α-glucosidase enzymatic activities. Methods: Gas chromatography–mass spectrometry (GC-MS) technique was engaged in the identification of phytochemical constituents. Antioxidant activities such as DPPH free radical scavenging ability, reducing power capacity, hydroxyl radical scavenging ability, singlet oxygen scavenging capacity, as well as α-amylase and α-glucosidase inhibition were carried out using standard in vitro methods. Results: GC-MS analysis of CFAm revealed the presence of 23 phytochemicals, out of which 5 compounds had the highest % compositions (i.e., octadecanoic acid (20.35%), 2-propanone, 1-(4-hydroxy-3-methoxyphenyl) (12.04%), isocomene (22.60%), 9, 12, 15 octadecatrienoic acid, methyl ester (Z,Z,Z) (28. 98%), and quercetin, 5TMS derivative (18.28%)). Also, CFAm demonstrated a significant (P<0.05) inhibition against DPPH (IC50= 26.33±1.39 mg/mL), with OH free radical scavenging capacity (65.46 ± 1.39 mg/100 g), singlet oxygen scavenging capacity (55.24 ± 1.22 mg/100 g), and showed ferric reducing power (84.52 ± 2.84 mg/100 g). Also, CFAm exhibited a significant (P<0.05) inhibition against α-glucosidase (IC50= 71.06 ±1.45 mg/ mL) and α-amylase (IC50= 73.88±1.58 mg/mL) in a concentration-dependent manners. Conclusion: The remarkable properties demonstrated by CFAm, which are essential for the management of DM, could probably be credited to the presence of the various identified phytonutrients.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45028340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Natural polyphenols, rutin, endogenous polycations, and polyamines (spermine and spermidine) are reported to have beneficial effects on hyperlipidemia, obesity, and cardiovascular disease (CVD). The present study attempts to evaluate the combined effects of polyamines or rutin and simvastatin on hyperlipidemic rats. Methods: Wistar rats were maintained on a high-fat diet (HFD) for 60 days. The HFD rats were administered from the 31st day onward with polyamines (PAs), rutin, and simvastatin for the next 30 days. The body weight, serum lipid profile, biomarkers, liver cholesterol, triglycerides, hydroxymethylglutaryl-CoA (HMG-CoA) reductase activity, antioxidants, and marker enzymes in HFD rats were estimated along with the liver histoarchitecture of the rats. Results: The experimental findings demonstrated abnormal alterations in body weight, serum lipid profile, hepatic lipid, and HMG-CoA reductase activity, hepatic antioxidants, serum marker enzymes, blood glucose, total protein, and histoarchitecture of the liver in HFD rats. The aforementioned treatment elicited a significant reduction in the biochemical parameters, biomarkers, and the evaluated enzymes in the present study. Furthermore, an improvement in the histoarchitecture of the liver was observed. Conclusion: The experimental results point out the positive role of antioxidant polyamines along with rutin on different parameters in HFD rats. Thus the combinatorial effect of polyamines and rutin with simvastatin (5 mg/kg) was found to be greater than simvastatin (10 mg/kg) alone. The enhancement in the anti-hyperlipidemic effect of simvastatin may be due to the intrinsic antioxidant property of polyamines and rutin.
{"title":"Influence of anti-hyperlipidemic activity by co-administration of polyamines and rutin with simvastatin","authors":"R. Patil, M. Aswar, S. Bhise, S. Naik","doi":"10.34172/jhp.2023.13","DOIUrl":"https://doi.org/10.34172/jhp.2023.13","url":null,"abstract":"Introduction: Natural polyphenols, rutin, endogenous polycations, and polyamines (spermine and spermidine) are reported to have beneficial effects on hyperlipidemia, obesity, and cardiovascular disease (CVD). The present study attempts to evaluate the combined effects of polyamines or rutin and simvastatin on hyperlipidemic rats. Methods: Wistar rats were maintained on a high-fat diet (HFD) for 60 days. The HFD rats were administered from the 31st day onward with polyamines (PAs), rutin, and simvastatin for the next 30 days. The body weight, serum lipid profile, biomarkers, liver cholesterol, triglycerides, hydroxymethylglutaryl-CoA (HMG-CoA) reductase activity, antioxidants, and marker enzymes in HFD rats were estimated along with the liver histoarchitecture of the rats. Results: The experimental findings demonstrated abnormal alterations in body weight, serum lipid profile, hepatic lipid, and HMG-CoA reductase activity, hepatic antioxidants, serum marker enzymes, blood glucose, total protein, and histoarchitecture of the liver in HFD rats. The aforementioned treatment elicited a significant reduction in the biochemical parameters, biomarkers, and the evaluated enzymes in the present study. Furthermore, an improvement in the histoarchitecture of the liver was observed. Conclusion: The experimental results point out the positive role of antioxidant polyamines along with rutin on different parameters in HFD rats. Thus the combinatorial effect of polyamines and rutin with simvastatin (5 mg/kg) was found to be greater than simvastatin (10 mg/kg) alone. The enhancement in the anti-hyperlipidemic effect of simvastatin may be due to the intrinsic antioxidant property of polyamines and rutin.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46194142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Othman El Faqer, S. Rais, I. Elkoraichi, Abdelaziz El Amrani, M. Dakir, Y. Zaid, E. Mtairag
Introduction: The leaves of Moroccan bay laurel (Laurus nobilis L.) have been used in several forms of extracts to cure rheumatic pain due to their anti-inflammatory properties. Our work aimed to evaluate the effects of aqueous and ethanolic extracts, as well as the essential oil (EO) from laurel, on the microbicidal activity of human neutrophils when compared to the effect of eucalyptol. Methods: The extracts (ethanolic and aqueous) were subject to phytochemical profiling and high-performance liquid chromatography (HPLC) analyses. The EO obtained by hydrodistillation from laurel was analyzed by gas chromatography-mass spectrometry (GC-MS). The immunomodulatory effects on neutrophil microbicidal activity of the extracts, EO, and eugenol were carried out by 3-(4,5-diméthylthiazol-2-yl)-2,5-diphényltétrazolium (MTT) assay. Results: The phytochemical analysis of the extracts revealed the presence of flavonoids, coumarins, phenols, flavone aglycones, and tannins. HPLC analysis showed the presence of numerous phenolic molecules such as syringic acid, ferulic acid, gallic acid, caffeine, and quercetin. The chemical composition of EO revealed that the major components were eucalyptol (44.14%), α-terpinyl acetate (11.11%), and β-phellandrene (6.74%). Aqueous and ethanolic extracts and EO revealed a significant and dose-dependent ability to inhibit neutrophils microbicidal activity with maximal inhibition at 200 µg/mL concentration with 30.42%, 24.7%, and 38.13%, respectively (P<0.001). Conclusion: The obtained results revealed the immunomodulatory properties of laurel as a potential natural anti-inflammatory agent that would also allow the development of new anti-inflammatory drugs.
简介:摩洛哥月桂(Laurus nobilis L.)的叶子因其抗炎特性已被用于几种形式的提取物中，以治疗风湿性疼痛。我们的工作旨在评估水提取物和乙醇提取物以及月桂精油(EO)对人体中性粒细胞杀微生物活性的影响，并将其与桉树精油的效果进行比较。方法:采用醇提液和水提液进行植物化学分析和高效液相色谱分析。采用气相色谱-质谱联用技术对月桂水蒸馏所得的环氧乙烷进行了分析。采用3-(4,5- dimsamthylthiazol -2-yl)-2,5- diphsamthylsamatzolium (MTT)法测定了丁香酚提取物、EO和丁香酚对中性粒细胞杀灭活性的免疫调节作用。结果:经植物化学分析，黄酮类化合物、香豆素类化合物、酚类化合物、黄酮类苷元和单宁类化合物均存在。高效液相色谱分析显示含有大量的酚类分子，如丁香酸、阿魏酸、没食子酸、咖啡因和槲皮素。其化学成分主要为桉油醇(44.14%)、α-松油酯乙酸酯(11.11%)和β-茶树烯(6.74%)。水提液、醇提液和EO对中性粒细胞的抑菌活性有显著的抑制作用，且呈剂量依赖性，在200µg/mL浓度下，其抑菌效果分别为30.42%、24.7%和38.13% (P<0.001)。结论:月桂树具有良好的免疫调节作用，是一种潜在的天然抗炎药物，为开发新型抗炎药物提供了基础。
{"title":"Phytochemical characterization and immunomodulatory effects of aqueous and ethanolic extracts and essential oil of Moroccan Laurus nobilis L. (Lauraceae) on human neutrophils","authors":"Othman El Faqer, S. Rais, I. Elkoraichi, Abdelaziz El Amrani, M. Dakir, Y. Zaid, E. Mtairag","doi":"10.34172/jhp.2023.08","DOIUrl":"https://doi.org/10.34172/jhp.2023.08","url":null,"abstract":"Introduction: The leaves of Moroccan bay laurel (Laurus nobilis L.) have been used in several forms of extracts to cure rheumatic pain due to their anti-inflammatory properties. Our work aimed to evaluate the effects of aqueous and ethanolic extracts, as well as the essential oil (EO) from laurel, on the microbicidal activity of human neutrophils when compared to the effect of eucalyptol. Methods: The extracts (ethanolic and aqueous) were subject to phytochemical profiling and high-performance liquid chromatography (HPLC) analyses. The EO obtained by hydrodistillation from laurel was analyzed by gas chromatography-mass spectrometry (GC-MS). The immunomodulatory effects on neutrophil microbicidal activity of the extracts, EO, and eugenol were carried out by 3-(4,5-diméthylthiazol-2-yl)-2,5-diphényltétrazolium (MTT) assay. Results: The phytochemical analysis of the extracts revealed the presence of flavonoids, coumarins, phenols, flavone aglycones, and tannins. HPLC analysis showed the presence of numerous phenolic molecules such as syringic acid, ferulic acid, gallic acid, caffeine, and quercetin. The chemical composition of EO revealed that the major components were eucalyptol (44.14%), α-terpinyl acetate (11.11%), and β-phellandrene (6.74%). Aqueous and ethanolic extracts and EO revealed a significant and dose-dependent ability to inhibit neutrophils microbicidal activity with maximal inhibition at 200 µg/mL concentration with 30.42%, 24.7%, and 38.13%, respectively (P<0.001). Conclusion: The obtained results revealed the immunomodulatory properties of laurel as a potential natural anti-inflammatory agent that would also allow the development of new anti-inflammatory drugs.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46899510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Nigella sativa L. is a widely used medicinal plant throughout the world. The low toxic effects and low price of this plant make it an excellent treatment choice for many diseases. The present study aims to investigate the hepatoprotective effect of N. sativa L. total oil (TO) and its neutral lipid fraction (NLF) via the estimation of circulating xanthine oxidoreductase (XOR) level and anti-XOR antibodies titer. Methods: Antiradical activities of TO and NLF, in vitro, were carried out using three reactive oxygen species (ROS), superoxide anion, hydroxyl radical, and hydrogen peroxide. In vivo study was conducted to determine the possible protective effects of TO and NLF against ethanol-induced hepatotoxicity in rats feeding Lieber-DeCarli liquid diet in both sera and liver homogenate. Before conducting the hepatoprotective effects, we assessed the toxicity of our extracts using the same animal model. The administrated doses of 400 mg/kg for TO and 300 mg/kg for NLF showed no toxic effects. Results: ELISA assay indicated a significant increase (P<0.001) in the level of XOR and the titer of anti-XOR antibodies in rats treated with ethanol compared to the control group. After treatment with TO and NLF, the titer of anti-XOR antibodies and the level of XOR decreased significantly compared to the control group. Conclusion: XOR plays an important role in alcohol liver pathologies as a major source of free radicals. In addition, TO and NLF have significant potential as liver protective agents and might be utilized as new antioxidant therapeutics.
{"title":"Nigella sativa L. oil: Study of its toxicity, antiradical activity, and effect on circulating xanthine oxidoreductase","authors":"M. Asma, Khither Hanane, Mosbah Camélia, Slimani Abdelkader, Mahrouk Abdelkader, Boucherit Hanane, Maameri Zaineb","doi":"10.34172/jhp.2023.12","DOIUrl":"https://doi.org/10.34172/jhp.2023.12","url":null,"abstract":"Introduction: Nigella sativa L. is a widely used medicinal plant throughout the world. The low toxic effects and low price of this plant make it an excellent treatment choice for many diseases. The present study aims to investigate the hepatoprotective effect of N. sativa L. total oil (TO) and its neutral lipid fraction (NLF) via the estimation of circulating xanthine oxidoreductase (XOR) level and anti-XOR antibodies titer. Methods: Antiradical activities of TO and NLF, in vitro, were carried out using three reactive oxygen species (ROS), superoxide anion, hydroxyl radical, and hydrogen peroxide. In vivo study was conducted to determine the possible protective effects of TO and NLF against ethanol-induced hepatotoxicity in rats feeding Lieber-DeCarli liquid diet in both sera and liver homogenate. Before conducting the hepatoprotective effects, we assessed the toxicity of our extracts using the same animal model. The administrated doses of 400 mg/kg for TO and 300 mg/kg for NLF showed no toxic effects. Results: ELISA assay indicated a significant increase (P<0.001) in the level of XOR and the titer of anti-XOR antibodies in rats treated with ethanol compared to the control group. After treatment with TO and NLF, the titer of anti-XOR antibodies and the level of XOR decreased significantly compared to the control group. Conclusion: XOR plays an important role in alcohol liver pathologies as a major source of free radicals. In addition, TO and NLF have significant potential as liver protective agents and might be utilized as new antioxidant therapeutics.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41506888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khadija Benamar, S. Ibnsouda Koraichi, K. Fikri-Benbrahim
Celtis australis is a deciduous plant used worldwide in folk medicine to treat various ailments, such as stomach disorders, cough, pimples, joint pain, amenorrhoea, rheumatism, menstrual disorders, and herpes. The present review aims to document and summarize different works regarding the ethnomedicinal uses, phytochemistry, and biological activities of different parts (fruits, barks, leaves, and seeds) of this medicinal plant. For data collection, Google scholar, Science direct, Scopus, PubChem, and PubMed databases were used. This study shows that C. australis contains several bioactive compounds, exhibiting various pharmacological activities mainly hepatoprotective, analgesic, anti-inflammatory, cytotoxic, antioxidant, and antimicrobial properties. Thus, C. australis is a promising plant that can be exploited in the treatment of various diseases.
{"title":"Ethnobotany, phytochemistry and pharmacological activities of Celtis australis: A review","authors":"Khadija Benamar, S. Ibnsouda Koraichi, K. Fikri-Benbrahim","doi":"10.34172/jhp.2023.05","DOIUrl":"https://doi.org/10.34172/jhp.2023.05","url":null,"abstract":"Celtis australis is a deciduous plant used worldwide in folk medicine to treat various ailments, such as stomach disorders, cough, pimples, joint pain, amenorrhoea, rheumatism, menstrual disorders, and herpes. The present review aims to document and summarize different works regarding the ethnomedicinal uses, phytochemistry, and biological activities of different parts (fruits, barks, leaves, and seeds) of this medicinal plant. For data collection, Google scholar, Science direct, Scopus, PubChem, and PubMed databases were used. This study shows that C. australis contains several bioactive compounds, exhibiting various pharmacological activities mainly hepatoprotective, analgesic, anti-inflammatory, cytotoxic, antioxidant, and antimicrobial properties. Thus, C. australis is a promising plant that can be exploited in the treatment of various diseases.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41852320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ficus elastica Roxb. ex Hornem is usually found in tropical and subtropical areas, used in traditional medicine for various health problems, including pain, rheumatism, diarrhea, hypertension, infection, skin allergies, anemia, wound, hernia, and hemorrhoids. This review aims to present the phytoconstituents and pharmacological activities of F. elastica. A literature search employing PubMed, Google Scholar, Semantic Scholar, and Library was done to retrieve the relevant articles. F. elastica is a good source of traditional medicine for the treatment of various types of diseases, especially for microbial infections and preeclampsia. Quercitrin and myricetrin, having strong antioxidant activity, as well as ficusamide, ficusoside B, elastiquinone, elasticoside, and elasticamide are compounds, which have potential to be developed as new drugs for these conditions. In sum, the data regarding the pharmacological and safety aspects of this plant and its components are still limited. However, F. elastica is a natural product that has beneficial to human health and might be a good source for the preparation of new drugs.
{"title":"Phytochemistry, traditional uses, and pharmacological activities of Ficus elastica Roxb. ex Hornem: A review","authors":"Anggun Selvya Arsyad, A. Nurrochmad, N. Fakhrudin","doi":"10.34172/jhp.2023.04","DOIUrl":"https://doi.org/10.34172/jhp.2023.04","url":null,"abstract":"Ficus elastica Roxb. ex Hornem is usually found in tropical and subtropical areas, used in traditional medicine for various health problems, including pain, rheumatism, diarrhea, hypertension, infection, skin allergies, anemia, wound, hernia, and hemorrhoids. This review aims to present the phytoconstituents and pharmacological activities of F. elastica. A literature search employing PubMed, Google Scholar, Semantic Scholar, and Library was done to retrieve the relevant articles. F. elastica is a good source of traditional medicine for the treatment of various types of diseases, especially for microbial infections and preeclampsia. Quercitrin and myricetrin, having strong antioxidant activity, as well as ficusamide, ficusoside B, elastiquinone, elasticoside, and elasticamide are compounds, which have potential to be developed as new drugs for these conditions. In sum, the data regarding the pharmacological and safety aspects of this plant and its components are still limited. However, F. elastica is a natural product that has beneficial to human health and might be a good source for the preparation of new drugs.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46593749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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