Pub Date : 2021-10-01DOI: 10.22059/IJHST.2021.317458.436
Elaheh Hashemidehkordi, S. N. Mortazavi, P. Azadi
Zantedeschia spp is an important flower in the ornamental plants market. Due to the high demand for this plant in the horticultural industry, it is indispensable to introduce an in vitro protocol for its mass propagation. For this aim, the tubers of calla lily were disinfected in a hot water bath with different temperatures (30, 35, 40, 45, and 50 oC) and duration (30 or 35 min). Then explants were cultured on MS medium with different combinations of 6-Benzylaminopurine (BAP) and Kinetin (Kin). Based on the obtained results, the highest disinfection percentage (more than 90%) was obtained at 45 oC for 35 min. Also, the highest proliferation rate (with an average of 15.33 and 14.32 in cv. Orania and cv. Sunclub, respectively) was observed in 2.5 mg L-1 BAP + 1.5 mg L-1 Kin. The highest rooting percentage (100% in both cultivars) and root number per explant (with an average of 4.00 and 3.03 in cv. Orania and Sunclub, respectively) was obtained in 0.5 mg L-1 Indole-3-acetic acid (IAA) + 0.1 mg L-1 Kin, but the highest root length (with an average of 120.0 and 106.6 mm in cv. Orania and Sunclub, respectively) was observed in 1.0 mg L-1 IAA + 0.1 mg L-1 Kin. In MS medium + 2.0 mg L-1 Indole-3-butyric acid (IBA) + 4% Sucrose, the highest number of tubers (with an average of 6.66 and 5.21 in cv. Orania and Sunclub, respectively) was formed. The highest fresh and dry weights (with an average of 948.33 and 851.33 mg in cv. Orania and Sunclub, respectively) of tuber were obtained in 2.0 mg L-1 IBA + 6% sucrose. The rooted and tuberous plants were adapted in the greenhouse successfully.
{"title":"An Efficient in vitro Propagation Protocol of Pot Calla lily (Zantedeschia spp cv. Orania and Sunclub) via Tuber Production","authors":"Elaheh Hashemidehkordi, S. N. Mortazavi, P. Azadi","doi":"10.22059/IJHST.2021.317458.436","DOIUrl":"https://doi.org/10.22059/IJHST.2021.317458.436","url":null,"abstract":"Zantedeschia spp is an important flower in the ornamental plants market. Due to the high demand for this plant in the horticultural industry, it is indispensable to introduce an in vitro protocol for its mass propagation. For this aim, the tubers of calla lily were disinfected in a hot water bath with different temperatures (30, 35, 40, 45, and 50 oC) and duration (30 or 35 min). Then explants were cultured on MS medium with different combinations of 6-Benzylaminopurine (BAP) and Kinetin (Kin). Based on the obtained results, the highest disinfection percentage (more than 90%) was obtained at 45 oC for 35 min. Also, the highest proliferation rate (with an average of 15.33 and 14.32 in cv. Orania and cv. Sunclub, respectively) was observed in 2.5 mg L-1 BAP + 1.5 mg L-1 Kin. The highest rooting percentage (100% in both cultivars) and root number per explant (with an average of 4.00 and 3.03 in cv. Orania and Sunclub, respectively) was obtained in 0.5 mg L-1 Indole-3-acetic acid (IAA) + 0.1 mg L-1 Kin, but the highest root length (with an average of 120.0 and 106.6 mm in cv. Orania and Sunclub, respectively) was observed in 1.0 mg L-1 IAA + 0.1 mg L-1 Kin. In MS medium + 2.0 mg L-1 Indole-3-butyric acid (IBA) + 4% Sucrose, the highest number of tubers (with an average of 6.66 and 5.21 in cv. Orania and Sunclub, respectively) was formed. The highest fresh and dry weights (with an average of 948.33 and 851.33 mg in cv. Orania and Sunclub, respectively) of tuber were obtained in 2.0 mg L-1 IBA + 6% sucrose. The rooted and tuberous plants were adapted in the greenhouse successfully.","PeriodicalId":15968,"journal":{"name":"Journal of Horticultural Science","volume":"83 1","pages":"343-351"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89680860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22059/IJHST.2020.296220.336
Parvaneh Mohammadi-Benaruiyeh, G. Sharifi-sirchi
Concerning highly restricted application of chemicals in postharvest technology of horticultural crops, it is necessary to introduce the safe methods for preserving food or methods of food preservation. This study aimed to improve quality and prolong storage life of strawberry fruit by application of grape seed and fruit skin extracts and to compare them with calcium chloride as a chemical. In this study, strawberry fruit was individually immersed in the 1% and 2% CaCl2 solutions, seed and skin extracts (1 and 2 mg L-1) and then placed in polyethylene packaging for 24 d at 5±1 °C. Measurements of firmness, titrable acid, pH, weight loss, total antioxidant capacity, total phenolic, anthocyanin, vitamin C, enzymes' activity including catalase, peroxidase, and polyphenol oxidase and decay were carried out at 0, 6, 12, 18, and 24 d of storage. All applied treatments caused a significant effect on measured parameters including weight loss, titratable acidity, decay percentage and firmness, maintenance of anthocyanin and vitamin C contents, total phenolic, and antioxidant capacity. However, grape skin extract and grape seed extract showed the best results. Therefore, it can be concluded that Shiraz dark grape seed and skin extracts have the potential to control the decay incidence, prolong the storage life and preserve of postharvest valuable attributes of strawberry.
{"title":"Grape Seed and Skin Extracts as Natural Preserving Agents on Strawberry Fruit","authors":"Parvaneh Mohammadi-Benaruiyeh, G. Sharifi-sirchi","doi":"10.22059/IJHST.2020.296220.336","DOIUrl":"https://doi.org/10.22059/IJHST.2020.296220.336","url":null,"abstract":"Concerning highly restricted application of chemicals in postharvest technology of horticultural crops, it is necessary to introduce the safe methods for preserving food or methods of food preservation. This study aimed to improve quality and prolong storage life of strawberry fruit by application of grape seed and fruit skin extracts and to compare them with calcium chloride as a chemical. In this study, strawberry fruit was individually immersed in the 1% and 2% CaCl2 solutions, seed and skin extracts (1 and 2 mg L-1) and then placed in polyethylene packaging for 24 d at 5±1 °C. Measurements of firmness, titrable acid, pH, weight loss, total antioxidant capacity, total phenolic, anthocyanin, vitamin C, enzymes' activity including catalase, peroxidase, and polyphenol oxidase and decay were carried out at 0, 6, 12, 18, and 24 d of storage. All applied treatments caused a significant effect on measured parameters including weight loss, titratable acidity, decay percentage and firmness, maintenance of anthocyanin and vitamin C contents, total phenolic, and antioxidant capacity. However, grape skin extract and grape seed extract showed the best results. Therefore, it can be concluded that Shiraz dark grape seed and skin extracts have the potential to control the decay incidence, prolong the storage life and preserve of postharvest valuable attributes of strawberry.","PeriodicalId":15968,"journal":{"name":"Journal of Horticultural Science","volume":"35 1","pages":"415-429"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84024360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22059/IJHST.2020.309629.394
Lavin Babaei, M. Sharifani, R. Darvishzadeh, N. Abbaspour, M. Henareh
To investigate photosynthetic response of some pear (Pyrus spp.) species to drought stress, a pot experiment was conducted using as factorial experiment based on completely randomized design (CRD) with three replication under greenhouse condition. The factors included five pear species including: P. biossieriana, P. communis, P. glabra, P. salicifolia and P. syriaca and three levels of drought stress [(100%, 60% and 30% of field capacity (FC)]. According to the obtained results, different levels of drought stress significantly restricted morphological and physiological responses in all studied species. Increasing drought stress intensity reduced leaf relative water content (RWC), net photosynthetic rate, stomatal conductance, transpiration rate and intercellular carbon dioxide concentration when compared to their values in control plants. However, root/shoot dry weight ratio, specific leaf weight and stomatal density per unit of area were increased. In P. glabra exposed to severe stress (30% of FC), the values of root/shoot dry weigh ratio (0.85 g), specific leaf weight (23 mg cm-2), stomata density per unit of area, relative water content (73%) and net photosynthetic rate (3.9 µmol CO2 m-2 s-1) were significantly higher than the other species. P. syriaca, P. salicifolia, P. biossieriana and P. communis were placed in the next ranks, respectively based on their response to drought. In conclusion, P. glabra is reported as a more effective species in mitigating the adverse effects of drought by boosting its protective mechanisms than the other pear species.
{"title":"Impact of Drought Stress on Photosynthetic Response of Some Pear Species","authors":"Lavin Babaei, M. Sharifani, R. Darvishzadeh, N. Abbaspour, M. Henareh","doi":"10.22059/IJHST.2020.309629.394","DOIUrl":"https://doi.org/10.22059/IJHST.2020.309629.394","url":null,"abstract":"To investigate photosynthetic response of some pear (Pyrus spp.) species to drought stress, a pot experiment was conducted using as factorial experiment based on completely randomized design (CRD) with three replication under greenhouse condition. The factors included five pear species including: P. biossieriana, P. communis, P. glabra, P. salicifolia and P. syriaca and three levels of drought stress [(100%, 60% and 30% of field capacity (FC)]. According to the obtained results, different levels of drought stress significantly restricted morphological and physiological responses in all studied species. Increasing drought stress intensity reduced leaf relative water content (RWC), net photosynthetic rate, stomatal conductance, transpiration rate and intercellular carbon dioxide concentration when compared to their values in control plants. However, root/shoot dry weight ratio, specific leaf weight and stomatal density per unit of area were increased. In P. glabra exposed to severe stress (30% of FC), the values of root/shoot dry weigh ratio (0.85 g), specific leaf weight (23 mg cm-2), stomata density per unit of area, relative water content (73%) and net photosynthetic rate (3.9 µmol CO2 m-2 s-1) were significantly higher than the other species. P. syriaca, P. salicifolia, P. biossieriana and P. communis were placed in the next ranks, respectively based on their response to drought. In conclusion, P. glabra is reported as a more effective species in mitigating the adverse effects of drought by boosting its protective mechanisms than the other pear species.","PeriodicalId":15968,"journal":{"name":"Journal of Horticultural Science","volume":"147 1","pages":"353-369"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78290579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22059/IJHST.2020.303657.373
Alireza Akbarpour, B. Kavoosi, Mehdi Hosseinifarahi, S. Tahmasebi, Sedigheh Gholipour
Due to the development of garlic cultivation, quantitative and qualitative evaluations of garlic ecotypes in different regions are important for breeding purposes. In this study, some vegetative and phytochemical traits of eight Iranian garlic ecotypes were assayed in a RCBD with three replications at Darab Agricultural Research Station, Iran during 2017-2018 growing season. The results showed that there is a significant difference among garlic ecotypes in terms of studied traits (P <0.01). In general, Tarom and Tafresh ecotypes showed the highest plant height, number of leaves, length of garlic leaves, leaf width, fresh weight, dry weight and the highest bulb diameter. Highest dry weight of garlic bulb (g) per plant was obtained in Darab (79.0 g), Tarom (75.5 g), and Talesh (75.0 g) ecotypes, with no significant difference among them (P ≥ 0.01). Hamedan and Kerman ecotypes contained higher allicin content, alliin content, TPC, TFC, and TAC than the other ecotypes. Cluster analysis divided ecotypes into three distinct groups. Talesh and Hamedan ecotypes had the lowest similarity (0.34) and Darab and Tafresh ecotypes had the highest similarity (0.97). It can be concluded that Tarom, Tafresh, Hamedan, and Kerman can be recommended for cultivation depending on the quantitative (Tarom and Tafresh) and qualitative (Hamedan and Kerman) goals of cultivation
{"title":"Evaluation of Yield and Phytochemical Content of Different Iranian Garlic (Allium sativum L.) Ecotypes","authors":"Alireza Akbarpour, B. Kavoosi, Mehdi Hosseinifarahi, S. Tahmasebi, Sedigheh Gholipour","doi":"10.22059/IJHST.2020.303657.373","DOIUrl":"https://doi.org/10.22059/IJHST.2020.303657.373","url":null,"abstract":"Due to the development of garlic cultivation, quantitative and qualitative evaluations of garlic ecotypes in different regions are important for breeding purposes. In this study, some vegetative and phytochemical traits of eight Iranian garlic ecotypes were assayed in a RCBD with three replications at Darab Agricultural Research Station, Iran during 2017-2018 growing season. The results showed that there is a significant difference among garlic ecotypes in terms of studied traits (P <0.01). In general, Tarom and Tafresh ecotypes showed the highest plant height, number of leaves, length of garlic leaves, leaf width, fresh weight, dry weight and the highest bulb diameter. Highest dry weight of garlic bulb (g) per plant was obtained in Darab (79.0 g), Tarom (75.5 g), and Talesh (75.0 g) ecotypes, with no significant difference among them (P ≥ 0.01). Hamedan and Kerman ecotypes contained higher allicin content, alliin content, TPC, TFC, and TAC than the other ecotypes. Cluster analysis divided ecotypes into three distinct groups. Talesh and Hamedan ecotypes had the lowest similarity (0.34) and Darab and Tafresh ecotypes had the highest similarity (0.97). It can be concluded that Tarom, Tafresh, Hamedan, and Kerman can be recommended for cultivation depending on the quantitative (Tarom and Tafresh) and qualitative (Hamedan and Kerman) goals of cultivation","PeriodicalId":15968,"journal":{"name":"Journal of Horticultural Science","volume":"39 1","pages":"385-400"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90142997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22059/IJHST.2021.313592.411
D. M. Gatahi, Felix Nyoro
Fresh Moringa oleifera leaves are very rich in phytonutrients, however the leaves are also highly perishable and require processing for increased shelf-life. The method of processing, specifically drying affects the nutritional value of the product. The present study therefore, analyzed the nutraceutical value and growth of toxic microbes when the leaves were dried under different conditions i.e. room temperature, greenhouse, 50% shade net, and in the oven at 60 oC for 4 h. The experiments were carried out at the Jomo Kenyatta University of Agriculture and Technology (JKUAT). The treatments were applied in triplicate and arranged on a completely randomized design (CRD). Data on nutritional value of dried Moringa leaves was subjected to analysis of variance (ANOVA) for parameterization and means separated using protected LSD0.05. The study showed that drying Moringa leaves under shade, room and greenhouse conditions significantly (P<0.05) affects the nutritional value of the product. The results showed highest levels of vitamin C, vitamin A, polyphenols and terpenoids when the leaves were dried under 50% shade net and room temperature conditions. In contrast, the glucosinolate content was significantly (P<0.05) higher when the leaves were dried instantly in the oven (9.1%/wt), followed by drying under greenhouse conditions (8.7%/wt) before oven drying. However, drying of Moringa leaves under shade before oven drying resulted in growth of toxic microbial organisms such as staphylococcus, yeast, E. coli and molds that can potentially affect the safety of the product. Finally, the drying conditions also significantly (P<0.05) affected the moisture content of the powder obtained. The results of this study form an important reference for small-holder Moringa growers and processors in the development of an optimal processing regime for high value Moringa powder.
{"title":"Effect of Drying Method on Volatile Nutraceuticals and Microbial Growth in Moringa oleifera","authors":"D. M. Gatahi, Felix Nyoro","doi":"10.22059/IJHST.2021.313592.411","DOIUrl":"https://doi.org/10.22059/IJHST.2021.313592.411","url":null,"abstract":"Fresh Moringa oleifera leaves are very rich in phytonutrients, however the leaves are also highly perishable and require processing for increased shelf-life. The method of processing, specifically drying affects the nutritional value of the product. The present study therefore, analyzed the nutraceutical value and growth of toxic microbes when the leaves were dried under different conditions i.e. room temperature, greenhouse, 50% shade net, and in the oven at 60 oC for 4 h. The experiments were carried out at the Jomo Kenyatta University of Agriculture and Technology (JKUAT). The treatments were applied in triplicate and arranged on a completely randomized design (CRD). Data on nutritional value of dried Moringa leaves was subjected to analysis of variance (ANOVA) for parameterization and means separated using protected LSD0.05. The study showed that drying Moringa leaves under shade, room and greenhouse conditions significantly (P<0.05) affects the nutritional value of the product. The results showed highest levels of vitamin C, vitamin A, polyphenols and terpenoids when the leaves were dried under 50% shade net and room temperature conditions. In contrast, the glucosinolate content was significantly (P<0.05) higher when the leaves were dried instantly in the oven (9.1%/wt), followed by drying under greenhouse conditions (8.7%/wt) before oven drying. However, drying of Moringa leaves under shade before oven drying resulted in growth of toxic microbial organisms such as staphylococcus, yeast, E. coli and molds that can potentially affect the safety of the product. Finally, the drying conditions also significantly (P<0.05) affected the moisture content of the powder obtained. The results of this study form an important reference for small-holder Moringa growers and processors in the development of an optimal processing regime for high value Moringa powder.","PeriodicalId":15968,"journal":{"name":"Journal of Horticultural Science","volume":"30 1","pages":"315-322"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77733774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22059/IJHST.2021.316115.431
Namrata Ghimire, A. Srivastava, D. Poudel, K. Gaire
To determine the suitability of different mulching materials for improving the yield of potato,a field experiment was conducted from January 2020 to May 2020 at Kavrepalanchowk, Nepal. The experiment was carried out in Randomized Complete Block Design (RCBD) with five treatments including: T1: silver plastic mulch, T2: black plastic mulch, T3: perforated black plastic mulch, T4: straw mulch, and T5: Control, with four replications. The experiment revealed that the highest tuber yield was obtained in silver plastic mulch (42.29tonne/ha) followed by perforated black plastic (41.04tonne/ha), black plastic (39.17tonne/ha), and straw (28.54tonne/ha) mulches, and the lowest yield was obtained in the Control treatment (21.46tonne/ha). Soil temperature was found to be influenced by the use of mulching materials with the highest soil temperature recorded under black plastic mulch, whereas the lowest soil temperature was detected under the Control treatment. The economic analysis of using different mulching materials showed the highest benefit/cost ratio by silver plastic mulch (3.63); followed by perforated black plastic mulch (3.53) and the lowest benefit/cost ratio was calculated for the Control (2.60). The present study, therefore, depicted silver plastic mulch followed by perforated black plastic mulch as the most effective mulching material for improving production of potato.
{"title":"Yield and Economic Analyses of Different Mulching Materials for Potato Production","authors":"Namrata Ghimire, A. Srivastava, D. Poudel, K. Gaire","doi":"10.22059/IJHST.2021.316115.431","DOIUrl":"https://doi.org/10.22059/IJHST.2021.316115.431","url":null,"abstract":"To determine the suitability of different mulching materials for improving the yield of potato,a field experiment was conducted from January 2020 to May 2020 at Kavrepalanchowk, Nepal. The experiment was carried out in Randomized Complete Block Design (RCBD) with five treatments including: T1: silver plastic mulch, T2: black plastic mulch, T3: perforated black plastic mulch, T4: straw mulch, and T5: Control, with four replications. The experiment revealed that the highest tuber yield was obtained in silver plastic mulch (42.29tonne/ha) followed by perforated black plastic (41.04tonne/ha), black plastic (39.17tonne/ha), and straw (28.54tonne/ha) mulches, and the lowest yield was obtained in the Control treatment (21.46tonne/ha). Soil temperature was found to be influenced by the use of mulching materials with the highest soil temperature recorded under black plastic mulch, whereas the lowest soil temperature was detected under the Control treatment. The economic analysis of using different mulching materials showed the highest benefit/cost ratio by silver plastic mulch (3.63); followed by perforated black plastic mulch (3.53) and the lowest benefit/cost ratio was calculated for the Control (2.60). The present study, therefore, depicted silver plastic mulch followed by perforated black plastic mulch as the most effective mulching material for improving production of potato.","PeriodicalId":15968,"journal":{"name":"Journal of Horticultural Science","volume":"2 1","pages":"323-334"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87739068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22059/IJHST.2021.309763.393
C. Mutebi, Davine Atieno Ondede
Leaf spot (Cercospora moricola, Cooke) is a disease that negatively influences the yield of mulberry (Morus alba L.) plants. To determine the effect of nitrogen levels on the incidence and severity of leaf spot an experiment was carried out on mulberry plants. The nitrogen levels included 0, 100, 200 and 300 kg ha-1, which were applied in two splits coinciding with the two rainy seasons. The study design was randomized complete block design (RCBD) with three replications. Determination of disease intensity involved scoring for disease intensity on a 1-5 Manandhar scale and calculation of the disease incidence were performed by expressing the number of infected leaves as a percentage of the total number of leaves. The values were translated to area under disease progress stairs (AUDPS). The means for AUDPS were subjected to analysis of variance (ANOVA) using PRO GLM in SAS and Fisher’s least significant difference (LSD) used to partition the means at p≤0.05. The results showed that as the rate of nitrogen application was increased, there was a corresponding decrease in AUDPS for disease incidence and a decrease in AUDPS for disease severity. From the obtained results it can be concluded that nitrogen at an application rate of 200 kg ha-1 is an effective approach to suppress Cercospora leaf spot of mulberry and can be recommended to the farmers, where this disease is a problem for cultivation of mulberry.
叶斑病(Cercospora moricola, Cooke)是一种影响桑树(Morus alba L.)产量的病害。为确定氮素水平对桑树叶斑病发病率和严重程度的影响，进行了氮素水平对桑树叶斑病发病率和严重程度的影响。施氮量分别为0、100、200和300 kg ha-1，分两个雨季施用。研究设计为随机完全区组设计(RCBD)， 3个重复。疾病强度的确定涉及在1-5 Manandhar量表上对疾病强度进行评分，并通过将感染叶片的数量表示为叶片总数的百分比来计算疾病发病率。数值转化为疾病进展阶梯面积(AUDPS)。AUDPS的均值采用SAS中的PRO GLM进行方差分析(ANOVA)，采用Fisher最小显著性差异(LSD)对均值进行划分，p≤0.05。结果表明，随着施氮量的增加，病害发生率的AUDPS相应降低，病害严重程度的AUDPS相应降低。结果表明，施氮量为200 kg hm -1是防治桑叶斑病的有效措施，可推荐给桑叶斑病严重困扰的农户。
{"title":"Effect of Nitrogen Nutrition on the Intensity of Cercospora Leaf Spot of Mulberry","authors":"C. Mutebi, Davine Atieno Ondede","doi":"10.22059/IJHST.2021.309763.393","DOIUrl":"https://doi.org/10.22059/IJHST.2021.309763.393","url":null,"abstract":"Leaf spot (Cercospora moricola, Cooke) is a disease that negatively influences the yield of mulberry (Morus alba L.) plants. To determine the effect of nitrogen levels on the incidence and severity of leaf spot an experiment was carried out on mulberry plants. The nitrogen levels included 0, 100, 200 and 300 kg ha-1, which were applied in two splits coinciding with the two rainy seasons. The study design was randomized complete block design (RCBD) with three replications. Determination of disease intensity involved scoring for disease intensity on a 1-5 Manandhar scale and calculation of the disease incidence were performed by expressing the number of infected leaves as a percentage of the total number of leaves. The values were translated to area under disease progress stairs (AUDPS). The means for AUDPS were subjected to analysis of variance (ANOVA) using PRO GLM in SAS and Fisher’s least significant difference (LSD) used to partition the means at p≤0.05. The results showed that as the rate of nitrogen application was increased, there was a corresponding decrease in AUDPS for disease incidence and a decrease in AUDPS for disease severity. From the obtained results it can be concluded that nitrogen at an application rate of 200 kg ha-1 is an effective approach to suppress Cercospora leaf spot of mulberry and can be recommended to the farmers, where this disease is a problem for cultivation of mulberry.","PeriodicalId":15968,"journal":{"name":"Journal of Horticultural Science","volume":"28 1","pages":"335-342"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78157501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22059/IJHST.2020.298478.348
H. Sarikhani, Mohammad-Sadegh Safariyan-Nejad
One of the most important problems of strawberry cultivation in temperate regions is winter cold injuries. Current study investigated impacts of foliar application of glycine betaine (GB) at 0, 0.5, 1, 2 and 4 mM concentrations on the cold hardiness of strawberry. The plants were divided into two groups: one group for evaluation of cold hardiness at temperatures of -6, -9, -12, -15 and -18 °C; and the other for study of some biochemical characteristics. Results showed that GB treatment increased soluble carbohydrate and proline concentrations in both leaf and crown tissues, total protein concentration in leaf, and relative water content in leaf as compared to those in control. Based on LT50 calculated from electrolyte leakage and tetrazolium staining test, the GB application increased cold hardiness in strawberry plant based on its concentration. The highest cold hardiness was found in the 2 mM GB concentration based on electrolyte leakage and tetrazolium staining tests at -13.3 and -15.3 °C. Meanwhile, the lowest values of cold hardiness were observed in the control treatments based on electrolyte leakage and tetrazolium staining tests at -10.2 and -11.0 °C. Significant correlations were found between soluble carbohydrate and proline concentrations in leaf and crown, and total protein concentration with LT50 calculated from electrolyte leakage and tetrazolium staining test. We conclude that application of 2 mM GBhas the capacity to increase the freezing tolerance of strawberry and could be used as a prophylactic tool to reduce winter cold injury.
冬季冷害是温带地区草莓栽培的重要问题之一。本研究研究了叶面施用0、0.5、1、2和4 mM浓度甜菜碱对草莓抗寒性的影响。将植物分为两组:一组在-6、-9、-12、-15和-18℃的温度下评估抗寒性;另一个用于研究一些生化特性。结果表明，与对照相比，GB处理提高了叶片和树冠组织可溶性碳水化合物和脯氨酸浓度、叶片总蛋白浓度和叶片相对含水量。根据电解液泄漏计算的LT50和四氮唑染色试验可知，GB处理在不同浓度下提高了草莓植株的抗寒性。在-13.3°C和-15.3°C条件下，电解质泄漏和四氮唑染色试验表明，2 mM GB浓度下的耐寒性最高。在-10.2和-11.0℃条件下，电解液泄漏和四氮唑染色试验表明，对照处理的抗寒性最低。叶片和树冠可溶性碳水化合物和脯氨酸浓度与总蛋白浓度呈显著相关，通过电解质泄漏和四氮唑染色试验计算LT50。结果表明，施用2 mM绿绿剂可提高草莓的抗冻能力，可作为预防草莓冬季冻害的手段。
{"title":"Improving of Winter Cold Hardiness by Glycine Betaine in Strawberry","authors":"H. Sarikhani, Mohammad-Sadegh Safariyan-Nejad","doi":"10.22059/IJHST.2020.298478.348","DOIUrl":"https://doi.org/10.22059/IJHST.2020.298478.348","url":null,"abstract":"One of the most important problems of strawberry cultivation in temperate regions is winter cold injuries. Current study investigated impacts of foliar application of glycine betaine (GB) at 0, 0.5, 1, 2 and 4 mM concentrations on the cold hardiness of strawberry. The plants were divided into two groups: one group for evaluation of cold hardiness at temperatures of -6, -9, -12, -15 and -18 °C; and the other for study of some biochemical characteristics. Results showed that GB treatment increased soluble carbohydrate and proline concentrations in both leaf and crown tissues, total protein concentration in leaf, and relative water content in leaf as compared to those in control. Based on LT50 calculated from electrolyte leakage and tetrazolium staining test, the GB application increased cold hardiness in strawberry plant based on its concentration. The highest cold hardiness was found in the 2 mM GB concentration based on electrolyte leakage and tetrazolium staining tests at -13.3 and -15.3 °C. Meanwhile, the lowest values of cold hardiness were observed in the control treatments based on electrolyte leakage and tetrazolium staining tests at -10.2 and -11.0 °C. Significant correlations were found between soluble carbohydrate and proline concentrations in leaf and crown, and total protein concentration with LT50 calculated from electrolyte leakage and tetrazolium staining test. We conclude that application of 2 mM GBhas the capacity to increase the freezing tolerance of strawberry and could be used as a prophylactic tool to reduce winter cold injury.","PeriodicalId":15968,"journal":{"name":"Journal of Horticultural Science","volume":"67 1","pages":"401-413"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84061576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.22059/IJHST.2021.311550.400
F. Hossain, S. Numan, S. Akhtar
This review is planned to discuss the cultivation technology, nutritional values and health benefits of Dragon fruit. Dragon fruit plant has wide adaptability and grown well in slightly heavy texture soils. Plants are commonly propagated by stem cuttings. Flower buds are emerging after 15-18 months of planting and required 28-30 days for blooming. This fruit is popular due to its nutritional value, which is a good source of minerals, glucose, fructose, dietary fiber and vitamins. It strengthens the immune system of the human body and is also used in the treatment of diabetes, heart diseases and in maintaining healthy body weight. The yield and nutritional value of Dragon fruit vary depending on the species, cultivation practices, growing area and harvesting time. Dragon fruit peel has a high potential to be used as a natural dye. Numbers of commercial growers are gradually increasing in different countries due to getting a lucrative price of their product in the markets. At present, little information is available on production aspects of Dragon fruit. Research on different aspects of cultivation and health benefits of this fruit can help to maximize the benefits to worldwide growers and consumers and to expand the market of Dragon fruit.
{"title":"Cultivation, Nutritional Value, and Health Benefits of Dragon Fruit (Hylocereus spp.): a Review","authors":"F. Hossain, S. Numan, S. Akhtar","doi":"10.22059/IJHST.2021.311550.400","DOIUrl":"https://doi.org/10.22059/IJHST.2021.311550.400","url":null,"abstract":"This review is planned to discuss the cultivation technology, nutritional values and health benefits of Dragon fruit. Dragon fruit plant has wide adaptability and grown well in slightly heavy texture soils. Plants are commonly propagated by stem cuttings. Flower buds are emerging after 15-18 months of planting and required 28-30 days for blooming. This fruit is popular due to its nutritional value, which is a good source of minerals, glucose, fructose, dietary fiber and vitamins. It strengthens the immune system of the human body and is also used in the treatment of diabetes, heart diseases and in maintaining healthy body weight. The yield and nutritional value of Dragon fruit vary depending on the species, cultivation practices, growing area and harvesting time. Dragon fruit peel has a high potential to be used as a natural dye. Numbers of commercial growers are gradually increasing in different countries due to getting a lucrative price of their product in the markets. At present, little information is available on production aspects of Dragon fruit. Research on different aspects of cultivation and health benefits of this fruit can help to maximize the benefits to worldwide growers and consumers and to expand the market of Dragon fruit.","PeriodicalId":15968,"journal":{"name":"Journal of Horticultural Science","volume":"22 1","pages":"259-269"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90705953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.22059/IJHST.2021.314572.423
Ivy Lynn Aoko, Dezzline A. Ondigo, A. Kavoo, C. Wainaina
We reported a simple colorimetric probe based on gold nanoparticles (AuNPs) for detecting Ralstonia solanacearum. The AuNPs were synthesized through reduction with citrate ion and characterized by ultraviolet-visible spectroscopy (UV-vis), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The freshly synthesized AuNPs were brick red due to an intense surface plasmon absorption band at 520 nm. Upon interaction with synthetic gibberellic acid (GA3), a bathochromic shift occurred in the surface plasmon resonance (SPR) peak of AuNPs to higher wavelengths. The 'eye-ball' limit of detection was 0.2 ppm. This shift was accompanied by a change in the color of the AuNPs from brick red to purple. Soil samples were collected from the rhizosphere of tomato plants, exhibiting bacterial wilt symptoms and pure cultures of Ralstonia solanacearum isolated using a modified Kelman’s TZC medium. Gibberellins (GA) were extracted from the culture of R. solanacearum using ethyl acetate and characterized using fourier transform infrared spectroscopy (FT-IR). AuNP solution aggregation was induced by GA-mediated R. solanacearum. A color change from brick red to purple was also observed. The results illustrated the use of both SPR wavelength-shift sensing and visual color change to detect molecules of biological relevance.
{"title":"A Gold Nanoparticle-Based Colorimetric Probe for Detection of Gibberellic Acid Exuded by Ralstonia solanacearum Pathogen in Tomato (Solanum lycopersicum L.)","authors":"Ivy Lynn Aoko, Dezzline A. Ondigo, A. Kavoo, C. Wainaina","doi":"10.22059/IJHST.2021.314572.423","DOIUrl":"https://doi.org/10.22059/IJHST.2021.314572.423","url":null,"abstract":"We reported a simple colorimetric probe based on gold nanoparticles (AuNPs) for detecting Ralstonia solanacearum. The AuNPs were synthesized through reduction with citrate ion and characterized by ultraviolet-visible spectroscopy (UV-vis), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The freshly synthesized AuNPs were brick red due to an intense surface plasmon absorption band at 520 nm. Upon interaction with synthetic gibberellic acid (GA3), a bathochromic shift occurred in the surface plasmon resonance (SPR) peak of AuNPs to higher wavelengths. The 'eye-ball' limit of detection was 0.2 ppm. This shift was accompanied by a change in the color of the AuNPs from brick red to purple. Soil samples were collected from the rhizosphere of tomato plants, exhibiting bacterial wilt symptoms and pure cultures of Ralstonia solanacearum isolated using a modified Kelman’s TZC medium. Gibberellins (GA) were extracted from the culture of R. solanacearum using ethyl acetate and characterized using fourier transform infrared spectroscopy (FT-IR). AuNP solution aggregation was induced by GA-mediated R. solanacearum. A color change from brick red to purple was also observed. The results illustrated the use of both SPR wavelength-shift sensing and visual color change to detect molecules of biological relevance.","PeriodicalId":15968,"journal":{"name":"Journal of Horticultural Science","volume":"40 1","pages":"203-214"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78840146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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