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Effect of Tragacanth on Some Morpho-physiological Characteristics Related to Black cumin (Nigella sativa L.) Yield Under Drought Stress Conditions 黄芪甲对黑孜然(Nigella sativa L.)相关形态生理特性的影响干旱胁迫下的产量
Pub Date : 2021-05-10 DOI: 10.22067/JHS.2021.69276.1033
H. Kiani, Y. Sohrabi
Introduction In fact, drought is stress that restricts the plant photosynthesis and causing changes in the chlorophyll content and damage to photosynthetic structures. One of the important reasons that environmental stresses such as drought reduce the growth and photosynthesis ability of the plant is a disturbance in the balance between production and removal of free oxygen radicals. Transpiration is a necessary process for photosynthesis and growth of plants but depending on the conditions may be harmful in some cases, so that the use of anti-transpirant can be one of the most effective methods for reducing the amount of water lost through transpiration and effective way to reduce the effects of drought stress on plants and adjustment the reduction of the yield due to water deficiency in arid and semi-arid regions. Climate change created in the world and the intensification of stresses caused by it, especially drought stress in arid and semi-arid regions such as Iran, make it difficult to achieve this goal. Therefore, finding strategies that can reduce the effects of water shortages on plant growth and yield and improve growth and yield can be very important. The aim of the present study was to investigate the effect of irrigation regimes and application of different concentrations of tragacanth (naturally dried exudate from some Astragalus species) on black cumin plant. Materials and Methods This research was carried out in a factorial experiment based on completely randomized design with three replications in a greenhouse of the Agriculture College of Kurdistan University in 2018. The experimental factors were including irrigation at three levels of 100% (full irrigation), 70% (mild drought stress), and 40% (severe drought stress) of field capacity of soil and spraying with tragacanth extract at six concentrations of 0, 1.25, 2.5, 5, 7.5, and 10 g/L. Spraying of this material was done using a back sprayer (Shark model) with a constant pressure of 2.4 bar and a volume of 250 liters of water per hectare. Before the data were analyzed, their normality test was performed using the Mini Tab software. After ensuring the normality of data, analysis of variance was performed using SAS ver. 9.3. LSD (Least significant difference) was used to compare the mean of treatments. The graphs are drawn using Excel software. Results and Discussion The results showed that increased drought stress intensity (irrigation reduction) led to the reduced leaf relative water content, Total chlorophyll content, efficiency of photosystem II, plant hight, number of capsules per plant, mean number of seeds per plant, biological yield and grain yield. The positive effects of tragacanth consumption on reducing and modifying the effects of drought stress on different levels of irrigation and different concentrations of tragacanth were different. In the present study, under full irrigation conditions, lower concentrations of tragacanth were useful, while in drought stress condit
实际上,干旱是一种限制植物光合作用,引起叶绿素含量变化和光合结构破坏的胁迫。干旱等环境胁迫降低植物生长和光合作用能力的重要原因之一是自由基的产生和清除平衡受到干扰。蒸腾作用是植物光合作用和生长所必需的过程,但在某些情况下,蒸腾作用可能是有害的,因此使用抗蒸腾剂是减少蒸腾失水量的最有效方法之一,也是减轻干旱胁迫对植物的影响,调节干旱半干旱区因缺水而减产的有效途径。世界上造成的气候变化及其造成的压力加剧,特别是伊朗等干旱和半干旱地区的干旱压力,使实现这一目标变得困难。因此,寻找能够减少水资源短缺对植物生长和产量的影响并提高生长和产量的策略是非常重要的。本研究的目的是探讨灌溉制度和不同浓度的黄芪(一些黄芪属植物的自然干燥分泌物)对黑孜然植物的影响。材料与方法本研究采用完全随机设计的三重复析因试验,于2018年在库尔德斯坦大学农学院温室进行。试验因子包括100%(完全灌溉)、70%(轻度干旱胁迫)和40%(严重干旱胁迫)田间土壤容量3个水平的灌溉,以及0、1.25、2.5、5、7.5和10 g/L浓度的石甲提取物喷施。这种材料的喷洒是使用背喷器(鲨鱼型)完成的,其恒定压力为2.4巴,体积为每公顷250升水。在分析数据之前,使用Mini Tab软件进行正态性检验。在确保数据的正态性后,使用SAS ver进行方差分析。9.3. 采用LSD (Least significant difference)来比较治疗的平均值。图表是用Excel软件绘制的。结果与讨论结果表明,干旱胁迫强度增加(灌溉减少)导致叶片相对含水量、叶绿素总含量、光系统ⅱ效率、株高、单株蒴果数、单株平均种子数、生物产量和籽粒产量降低。不同灌溉水平和不同黄芪浓度下,黄芪消耗对减轻和调节干旱胁迫效应的正向作用不同。在本研究中,在充分灌溉条件下,低浓度的黄芪甲素是有益的,而在干旱胁迫条件下,高浓度的黄芪甲素(10 g/L除外)是有益的。在全灌条件下,1.25 g/L浓度对所有性状均呈阳性。在轻度干旱胁迫下,使用高浓度(5 g/L)的黄花色素效果最好,浓度越高,对所研究性状的影响越大。在严重干旱胁迫下,当浓度达到7.5 g/L时,黄芪提取物有利于改善这些性状,但10 g/L对这些性状有负面影响。考虑在重度和中度干旱胁迫条件下,适当浓度处理对黄芪试验性状的改善和籽粒产量的提高效果分别比不施用黄芪提高11.6%和28.2%。结论不同浓度、不同灌溉水平的黄花胶物质对黑孜然性状的影响存在差异。因此,可以得出结论,不同浓度的黄甲胶的施用完全取决于植物的水分状况。因此,在干旱胁迫条件下使用高浓度的黄花胶对植物有更积极的影响,反之亦然,在充分灌溉条件下使用低浓度的黄花胶是有用的。黄甲胶在不同植物中减轻和调节干旱胁迫效应的作用有待进一步研究和广泛研究。黄芪胶是一种天然来源的新型抗蒸腾剂,在干旱地区具有广泛的应用价值。
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引用次数: 0
Reaction of Sweet Bail (Ocimum bacilicum L.) to Different Fertilizing System in Organic Farming 有机农业中不同施肥制度对甜蜜的影响
Pub Date : 2021-05-08 DOI: 10.22067/JHS.2021.61907.0
Soheila Charbandi, F. Zaefarian, V. Akbarpour, M. Kaveh
Introduction: The tendency to produce aromatic and medicinal plants and the demand for natural products is increasing especially in the world of ecological agriculture. Ecological agriculture of medicinal plants guarantees their quality and reduces the possibility of negative effects on their medicinal quality and yield. One of the most important medicinal plant species is basil, which is widely used in food and pharmaceutical industries. Common basil (Ocimum bacilicum L.) is the most important economic species of Ocimum and is cultivated and consumed in almost all warm and temperate regions. Evaluation of different plant nutrition systems is one of the important principles in the production planning of medicinal plants in order to achieve high yield and quality of effective ingredients. Proper fertilizer management is one of the important factors in agricultural production. Identification of fertilizers compatible with nature and suitable for plant growth can have favorable effects on quantitative and qualitative indices of the plant. Organic fertilizers are genuine and risk-free products that can be suitable for agricultural sustainability. Soil organic matter improves fertility and water retention, provides proper ventilation, long-term storage of plant nutrients and microorganisms, so replacing chemical fertilizers with fertilizers such as vermicompost and biofertilizer can reduce the above-mentioned effects. Biofertilizers are one of the operations that is nowadays in accordance with the principles of sustainable agriculture for soil fertility. Biofertilizers include the compost of one or more types of useful soil organisms or as metabolic by-products of these organisms that are used to provide the plant with the nutrients it needs in an agroecosystem.Materials and Methods: In order to investigate the effect of biochar and organic and biological fertilizers on the quantity and quality of Ocimum bacilicum L., a factorial experiment was done in a randomized complete block design with three replications. Experimental treatments included two levels of biochar (0 and 20 ton ha-1) and five levels of fertilizers (vermicompost (10 ton ha-1), Mycorrhiza, Azotobacter, Pseudomonas and no fertilizer application). Prior to planting sweet basil seed, biofertilizers and vermicompost were mixed with soil to the depth of 15 cm. Mycorrhizal fungi were mixed with seed. Azotobacter and Pseudomonas bacteria were also incubated with seeds (seed inoculated). It should be added that in this study no chemicals were used to control weeds and pests. Before flowering stage, morphological traits of basil (plant height, stem diameter and number of branches) were measured. Also, to measure the dry weight of the shoots, basil was scalped from one square meter and weighed, and their fresh weight was thus determined. Then, to determine the dry weight, the samples were transferred to an oven at 70 ° C for 48 hours and finally weighed. Also in the flowering stage, the amount o
引言:芳香植物和药用植物生产的趋势和对天然产物的需求日益增加,特别是在世界生态农业中。药用植物生态农业保证了药用植物的品质,减少了对药用植物品质和产量产生负面影响的可能性。罗勒是最重要的药用植物之一,广泛应用于食品和制药工业。普通罗勒(Ocimum bacilicum L.)是罗勒属植物中最重要的经济品种,几乎在所有温暖和温带地区都有种植和消费。对不同植物营养系统进行评价是药用植物生产计划的重要原则之一,以实现有效成分的高产和优质。合理的肥料管理是农业生产的重要因素之一。鉴定与自然相容、适合植物生长的肥料,对植物的定量和定性指标都有良好的影响。有机肥是货真价实的无风险产品,适合农业可持续发展。土壤有机质提高肥力和保水性,提供适当的通风,长期储存植物养分和微生物,因此用蚯蚓堆肥和生物肥料等肥料代替化学肥料可以减轻上述影响。生物肥料是当今符合可持续农业土壤肥力原则的操作之一。生物肥料包括一种或多种有用土壤生物的堆肥,或作为这些生物的代谢副产物,用于为农业生态系统中的植物提供所需的营养。材料与方法:采用随机完全区组设计,采用3个重复的因子试验,研究生物炭与有机肥和生物肥对芽孢杆菌数量和品质的影响。试验处理包括2个水平的生物炭(0和20吨hm -1)和5个水平的肥料(蚯蚓堆肥(10吨hm -1)、菌根、固氮菌、假单胞菌和不施肥)。在种植甜罗勒种子之前,将生物肥料和蚯蚓堆肥与土壤混合至15厘米深。菌根真菌与种子混合。固氮菌和假单胞菌也用种子孵育(接种种子)。应该补充的是,在本研究中没有使用化学药品来控制杂草和害虫。在开花期前,对罗勒植株的形态性状(株高、茎粗、分枝数)进行了测定。此外,为了测量嫩芽的干重,从一平方米的罗勒剥下头皮并称重,从而确定其新鲜重量。然后,为了确定干重,将样品转移到70°C的烤箱中放置48小时,最后称重。在花期,测定了总酚、总黄酮含量和抗氧化活性(DPPH)。最后使用SAS统计软件对数据进行分析,并使用Excel软件绘制图表。在5%的水平下,采用LSD检验比较均数。结果与讨论:结果表明,蚯蚓堆肥生物炭处理的主枝高度和主枝数最高(分别为49.37 cm和7个),显著优于其他处理。生物炭与肥料的交互作用对植株叶片和茎干鲜重有显著影响。各处理平均比较结果表明,生物炭+假单胞菌处理的叶片鲜重和茎鲜重最高,分别为792和876 g m-2,与生物炭+蚯蚓堆肥处理差异不显著;施用假单胞菌的生物炭叶片干重和茎干重最大(分别为166和175 g m-2)。生物炭与肥料互作对罗勒酚、黄酮类化合物和抗氧化活性的影响在处理间存在显著差异。结论综上所述,生物炭和生物有机肥的施用对甜瓜的质量和数量都有一定的影响,其中偶氮杆菌和假单胞菌与生物炭的施用对甜瓜的质量和数量影响最大。综上所述,在生态或低投入农业中,生物炭与生物肥料配施对甘蔗渣产量的数量和质量增长均有显著影响。
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引用次数: 0
Evaluation of the Effect of Biochar Obtained from Different Pyrolysis Temperatures on the Marigold Growth under Salt Stress 盐胁迫下不同热解温度生物炭对万寿菊生长影响的评价
Pub Date : 2021-04-27 DOI: 10.22067/JHS.2021.67776.1005
Amin Shamsaddin Saied, M. Ramroudi
Background and objectives: Biochar is a carbonaceous substance obtained from heating plant residues and wastes in an oxygen-containing medium with or without oxygen. Thermal decomposition of biomass in an oxygen-free medium is called thermophilicity (pyrolysis). Temperature is one of the factors influencing the characteristics of biochar. One of the objectives of this experiment is to investigate the effect of different temperatures on the biochar characteristics of cattle manure. The use of biochar as a soil conditioner and source of organic carbon in agricultural soils with minimal environmental damage is considered. Marigold is an annual plant that is used in industry and pharmacy in addition to food. Another aim of this experiment is to investigate the effect of biochar use from different temperatures on marigold under salinity stress.Materials and Methods: For biochar preparation, after collecting cattle manure from Bardsir farms, air drying and sieving for four hours at temperatures (300, 400, 500, 600 ° C) were performed for pyrolysis process. Then pH, EC, carbon stability, ash and biochar performance were measured. In order to evaluate the effects of biochar resulting from different heat-treated temperatures on salinity tolerance of marigold, a factorial experiment was conducted in a completely randomized design in the greenhouse. The two factors studied included salinity levels (0, 4, 8 and 12 dS / m) and biochar resulting from different thermocouple temperatures (0, 300, 400, 500 and 600° C). The biochar rate was considered to be 20%. One month after salinity treatment, seedlings were evaluated for osmotic metabolites activity and growth characteristics of marigold seedlings.Results discussion: The results of analysis of variance showed that all biochar properties were significantly affected by temperature factor. With increasing the pyrolysis temperature to 600 ° C, pH and EC increased by 16.29% and 60.37%, respectively, and the ash content increased by 1.5 times, but biochar performance and bulk density decreased by 52.28% and 48.1%, respectively. The highest carbon stability was observed at 500 ° C, which increased by 20% compared to 300 ° C. The results showed a significant negative effect of salinity stress on stem height, number and area of marigold leaves and with increasing salinity to 12 dS / m, 31.09, 17.28 and 45.7% decrease in these traits were observed, respectively. The physiological characteristics of marigold were significantly affected by the simple and interaction effects of salinity and biochar stress. In treatments 0, 4, 8 and 12 dS/m with increasing pyrolysis temperature to 600 ° C respectively 2.2, 2.04, 1.97 and 1.92 times increase in leaf potassium concentration and 1.54, 2.26, 3.00 and 2.45 times higher than the control treatment in the amount of leaf proline was observed but the amount of protein showed the opposite trend. The activities of catalase, ascorbate peroxidase and guaiacol peroxidase enzymes were als
背景和目的:生物炭是一种碳质物质,通过在含氧或无氧介质中加热植物残余物和废物而获得。生物质在无氧介质中的热分解称为亲热性(热解)。温度是影响生物炭特性的因素之一。本试验的目的之一是研究不同温度对牛粪生物炭特性的影响。生物炭在农业土壤中作为土壤调理剂和有机碳源,对环境破坏最小。万寿菊是一种一年生植物,除食品外,还用于工业和制药。本实验的另一个目的是研究不同温度下使用生物炭对盐胁迫下万寿菊的影响。材料与方法:制备生物炭,从Bardsir农场收集牛粪后,在300、400、500、600℃温度下风干、筛分4小时,进行热解处理。然后测定pH、EC、碳稳定性、灰分和生物炭性能。为了评价不同热处理温度下生物炭对万寿菊耐盐性的影响,采用完全随机设计的温室析因试验。研究的两个因素包括盐度水平(0、4、8和12 dS / m)和不同热电偶温度(0、300、400、500和600°C)产生的生物炭,生物炭率认为为20%。盐度处理1个月后,对万寿菊幼苗的渗透代谢物活性和生长特性进行了评价。结果讨论:方差分析结果表明,温度因子对生物炭的各项性能均有显著影响。当热解温度提高到600℃时,pH和EC分别提高了16.29%和60.37%,灰分含量提高了1.5倍,但生物炭性能和容重分别下降了52.28%和48.1%。结果表明,盐度胁迫对金盏花茎高、叶数和叶面积均有显著的负影响,盐度升高至12 dS / m时,金盏花茎高、叶数和叶面积分别降低31.9%、17.28%和45.7%。盐度和生物炭胁迫对万金菊生理特性的影响是显著的。在0、4、8和12 dS/m处理下,随着热解温度升高至600℃,叶片钾浓度分别比对照提高2.2、2.04、1.97和1.92倍,叶片脯氨酸含量比对照提高1.54、2.26、3.00和2.45倍,而蛋白质含量则相反。过氧化氢酶、抗坏血酸过氧化物酶和愈创木酚过氧化物酶的活性也受到盐胁迫和热温度的显著影响。600℃时,过氧化氢酶活性最高,抗坏血酸过氧化物酶和愈创木酚过氧化物酶活性最高,分别为8 dS/m和4 dS/m。结论:总体而言,生物炭的盐度是其最重要的不良性质,且随着热解温度的升高而增加,因此是否推荐在盐渍土中使用生物炭还有待进一步研究。在本研究中,盐胁迫下使用生物炭对万金菊的抗性和耐盐性没有显著的正向影响。关键词:热解温度,粪便,过氧化氢酶,抗坏血酸过氧化物酶,钾。背景和目的:生物炭是一种碳质物质,通过在含氧或无氧介质中加热植物残余物和废物而获得。生物质在无氧介质中的热分解称为亲热性(热解)。温度是影响生物炭特性的因素之一。本试验的目的之一是研究不同温度对牛粪生物炭特性的影响。生物炭在农业土壤中作为土壤调理剂和有机碳源,对环境破坏最小。万寿菊是一种一年生植物,除食品外,还用于工业和制药。本实验的另一个目的是研究不同温度下使用生物炭对盐胁迫下万寿菊的影响。材料与方法:制备生物炭,从Bardsir农场收集牛粪后,在300、400、500、600℃温度下风干、筛分4小时,进行热解处理。然后测定pH、EC、碳稳定性、灰分和生物炭性能。
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引用次数: 0
Evaluation of Clover Lawns as a Substitute of Common Grass Lawns in Landscape to Reduce the Moving Costs 三叶草草坪在景观中替代普通草坪降低迁移成本的评价
Pub Date : 2021-04-05 DOI: 10.22067/JHS.2021.60852.0
Elham SaeediPooya, A. Tehranifar, G. A. Gazanchian, F. Kazemi, M. Shoor
Introduction Turfgrasses, as one of the important components of urban landscapes, have played a vital role in this regard. Turfgrasses have provided functional, recreational, and aesthetic benefits to urban landscapes. Multiple benefits of turf are well documented such as recreational health, enhanced water infiltration, erosion control, mediation in heat-island effects and reduction in nutrient leaching. But the main problems of turfgrass development are the cost of turfgrass seeds, maintenance costs such as moving, as well as the high water requirement in arid and semi-arid regions. Thus, the reduction of lawn culture in the landscape is one of the decision has taken in parks and green spaces organization of Tehran, Isfahan and even Mashhad. Materials and Methods So the aim of this experiment was to compare three common turfgrasses with two clover varieties as turf replacement to reduce landscape maintenance cost specially moving cost. So, some growth factors of clover lawn: Trifolium repens var. Calway and Trifolium repens var. Pipolina (micro clover) and three turfgrasses of Festuca arundinacea L. and Lolium perenneL. and commercial sport turf mixture (from NAk-Nederland Ltd.) were compared together. This research project was conducted in randomized complete block design with three replications in the research field of the Department of Horticultural Science and Landscape Engineering‚ Agricultural Faculty‚ Ferdowsi University of Mashhad‚ Mashhad‚ Iran, during 2016 and 2017. The site (59o 38′ E and 36o 16 ′ N; elevation 989 m) is located in an arid and semi- arid region with mean annual rainfall 233.8 mm and long term averages of maximum and minimum temperature are 22.5 oC and 9.3 oC‚ respectively. Turfgrass plots were established by directly sowing the seeds in April, 2016. The planting rate of the seedling considering their pure live seeds (PLS) were 45 g.m-2 for Lolium perenne, 34 g.m-2 for Festuca arundinacea, 6.5 g.m-2 for Trifolium repens, 5.5 g.m-2 for micro clover and 39 g.m-2 for commercial mixture of sport turf. The plots were 1 m2 (1m×1m) in size and were prepared after plowing and leveling the soil. The seeds were hand sown and covered with a thin layer of leaf compost and sand. Results and Discussion According to the results in the first evaluation of emergence percentage, Lolium perenne had the highest emergence percentage. But, after 36 days from culture, all plants had 92-98 % coverage which did not have any significant difference among grasses with clover lawns. In terms of density, uniformity and weed density, there was no significant difference between the studied plants. In the other hands, white clover showed the best quality after clipping in both years. At the point of growth index, which have done in this experiment by measuring height and dry weight of clipping, grass lawns had the higher growth index in compered to clover lawn in both years. Festuca arundinacea and Lolium perenne had the highest growth index and white
草坪草作为城市景观的重要组成部分之一,在这方面发挥着至关重要的作用。草坪草为城市景观提供了功能性、娱乐性和美学上的好处。草皮的多种好处是有据可查的,如娱乐健康、增强水渗透、控制侵蚀、调解热岛效应和减少养分淋失。但草坪草发展的主要问题是草坪草种子的成本、搬迁等维护成本,以及干旱和半干旱地区的高需水量。因此,减少景观中的草坪文化是德黑兰、伊斯法罕甚至马什哈德的公园和绿地组织所采取的决定之一。因此,本试验的目的是比较三种常见的草坪草与两种三叶草品种替代草坪,以降低景观维护成本,特别是搬迁成本。因此,三叶草草坪的一些生长因子:三叶草和微三叶草,以及三种草坪草羊茅和多年生黑麦草。与商用运动草坪混合料(NAk-Nederland Ltd.)进行了比较。本研究项目于2016年至2017年在伊朗马什哈德费尔多西大学农学院园艺科学与景观工程系进行了随机完全区组设计,共有3个重复。场地(东经59°38′,北纬36°16′;海拔989 m),地处干旱半干旱区,年平均降雨量233.8 mm,长期平均最高气温22.5 oC,最低气温9.3 oC。2016年4月,通过直接播种建立了草坪草坪地块。考虑其纯活种子(PLS),种苗播种率为:多年生黑麦草45 g·m-2,黄羊茅34 g·m-2,三叶草6.5 g·m-2,微型三叶草5.5 g·m-2,运动草坪商业混合料39 g·m-2。这些地块面积为1平方米(1m×1m),是在翻耕和平整土壤后准备的。这些种子是手工播种的,上面覆盖着一层薄薄的树叶堆肥和沙子。结果与讨论根据第一次羽化率评价结果,黑麦草的羽化率最高。但培养36 d后,所有植物的覆盖度为92% ~ 98%,与三叶草草坪间差异不显著。在密度、均匀度和杂草密度方面,各研究植物间无显著差异。另一方面,白三叶草在两年中均表现出最好的剪枝质量。在生长指数点上,本试验通过测量剪枝高度和干重得出,两年内,草皮草坪的生长指数均高于三叶草草坪。羊茅和黑麦草的生长指数最高,白三叶草和微型三叶草的生长指数最低。同时,随着气候变暖,羊茅在株高和剪枝干重上成为优势植物。7月,羊茅的剪枝干重最高,为40 g .m-2,其次是黑麦草(19.52 g .m-2)、商业运动草坪(15.68 g .m-2)、白三叶(6.24 g .m-2)和微型三叶草(0.36 g .m-2)。景观的问题之一是减少搬迁等维护成本。因此,与草坪草相比,白三叶草和微型三叶草的生长速度较低是降低搬家成本的积极因素。三叶草草坪的覆盖度和适宜密度与普通草坪相似,差异不显著。白三叶草和微型三叶草的低生长特性可以有效地降低灌溉成本,这需要进一步的研究和检验。这是白三叶草和微型三叶草的一个积极点,不需要每个月移动或切割。几乎,三叶草移动是建议移除他们的花。因此,在园林绿化中建议使用白色和微型三叶草作为维护费用较少的替代品。最后,本研究中草和三叶草单作栽培的结果可用于生产适合我国(干旱和半干旱地区)气候的三叶草-草混合物,而不是从欧洲国家进口草坪混合物。
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引用次数: 0
The Study of Micromorphological and Essential oil Analysis of Nepeta binaloudensis and Nepeta cataria (Lamiaceae) 双花荆芥和野荆芥的显微形态及精油分析研究
Pub Date : 2021-04-05 DOI: 10.22067/JHS.2021.60692.0
Fatemehzahra Amirmohammadi, M. Azizi
Introduction The Nepeta is one of the largest genus of Nepetoide subfamily, in the Lamiaceae family with about 79 species in Iran. Most of the species are perennial rarely annual with different vegetative forms, leaves covered with trichome. Glandular trichomes are widely distributed all over the aerial part of Nepeta species, and their largely to its great importance and their structures can vary widely among plants species. Some species contain monoterpenes, sesquiterpenes, cyclopentanoid iridoids derivatives and nepetalactones which they are used in folk medicine as, diaphoretic, diuretic, antitussive, febrifuge agent. In the present work, scanning electron microscopy (SEM) was used to determine the nutlet micromorphology and also micromorphology and distribution of the glandular trichomes of Nepeta binaludensis “an Iranian endemic species” and Nepeta cataria in order to improve the knowledge of the species and to evaluate the usefulness of this feature for phytochemical and systematic purpose. The chemical composition of its essential oil was also analyzed. This paper provides a detailed description of trichome morphology and nutlet in Nepeta binaludensis andNepeta catariaspecies growing in Iran, by scanning electron microscopy (SEM), and evaluates the systematic significance of such characteristics.   Materials and Methods The research was conducted in research garden of Agricultural Faculty of Ferdowsi University of Mashhad during 2017. Seeds of two Nepeta species were provided by Institute of Plant Sciences in Ferdowsi University of Mashhad. The seeds were cultivation in greenhouse and the seedling (10 cm long) were transplanted in the field. During the summer aerial parts of the plants were harvested at flowering stage, dried in shade, and (30 g) were hydro distilled for 3 h using Clevenger apparatus. For SEM study, leaves were fixed with 3% glutaraldehyde in 0.1 M sodium phosphate buffer, pH 7.2 for 30 min at 4 °C. After washing in water the material was dehydrated through an ethanol gradient and critical point dried. Specimens were mounted onto SEM stubs using double-sided adhesive tape and coated with palladium. For nutlets observation, seed were directly mounted onto aluminum stubs using double-sided adhesive tape and coated with palladium and photographed.   Result Based on the obtained data, the surface leaves of both species (N. binaludensis and N.cataria) was observed forglandular and non-glandular trichomes distribution. (The glandular trichomes of the peltate types). Trichome density and size was variable as 7-21mm2 leaf surface (63-77 µm) in N. binaludensis and as 20-30 mm2 leaf surface (47-67 µm) in N.cataria. In both species only one types of glandular trichome was identified. Peltate trichomes comprise of a basal cell situated in the leaf epiderm with a four-celled secretory head. Nutlets of Nepeta were blackish-brown or brown in color. One type surface ornamentation, can be distinguished: smooth-type.  The nutlet o
Nepeta是Lamiaceae中Nepetoide亚科最大的属之一,在伊朗约有79种。大多数种是多年生很少一年生与不同的营养形式,叶被毛。腺毛广泛分布于Nepeta属植物的地上部分,由于其重要性及其结构在不同植物种类间差异很大。有些品种含有单萜、倍半萜、环戊烷类环烯醚萜类衍生物和荆内酯,在民间医药中用作发汗剂、利尿剂、止咳剂、退热剂。本文利用扫描电子显微镜(SEM)对伊朗特有种Nepeta binaludensis和Nepeta cataria的坚果微形态和腺毛的微形态和分布进行了研究,以提高对该物种的认识,并评估这一特征在植物化学和系统目的上的有用性。并对其精油的化学成分进行了分析。本文利用扫描电子显微镜(SEM)对生长在伊朗的Nepeta binaludensis和Nepeta catariensis的毛状体形态和坚果进行了详细的描述,并评价了这些特征的系统意义。材料与方法研究于2017年在马什哈德费尔多西大学农学院研究园进行。马什哈德Ferdowsi大学植物科学研究所提供了两种Nepeta的种子。种子在温室栽培,幼苗(长10cm)移栽于田间。在夏季,植物的地上部分在开花阶段收获,在阴凉处干燥,并(30 g)使用Clevenger装置进行3小时的水力蒸馏。扫描电镜研究,叶片用3%戊二醛固定在0.1 M磷酸钠缓冲液中,pH为7.2,在4℃下固定30分钟。在水中洗涤后,物料通过乙醇梯度脱水并进行临界点干燥。用双面胶带将标本装在SEM存根上,并涂上钯。为了观察坚果,种子直接用双面胶带贴在铝桩上,涂上钯并拍照。结果根据所获得的数据,观察了两种植物(N. binaludensis和N.cataria)叶片表面腺毛和非腺毛的分布。(胸骨型的腺毛)。毛状体的密度和大小变化较大,分别为7 ~ 21mm2叶面(63 ~ 77µm)和20 ~ 30 mm2叶面(47 ~ 67µm)。在这两个物种中只鉴定出一种腺毛。盾状毛由位于叶表皮的一个基底细胞和一个四细胞的分泌头组成。尼佩塔的坚果是黑棕色或棕色的。一种类型的表面纹饰,可区分为:光滑型。小坚果的装饰图案以细粒状的光滑型为主,而小坚果的装饰图案以网状型为主。采用气相色谱(GC)和质谱联用(GC/MS)对大田栽培的两种植物地上部的水蒸馏精油进行了分析。在白莲挥发油中分别鉴定出51种和26种成分。白莲挥发油的主要成分为1.8-桉叶油素(14.8%)、β-蒎烯(10.4%)和对伞花油素(9.8%)。荆芥挥发油的主要成分为4 -a、7-a、7a-b-荆芥内酯(72/76%)和4 -a、7-a、7a-a-荆芥内酯(17.86%)。根据在精油中鉴定的关键化合物的组成,Nepeta物种被分为两组。结论根据其挥发油中主要化合物的组成,可将其分为两类。在我们的研究基础上,binaludensis属于1.8-桉树脑化学型,而n.c aria属于nepetalone化学型。木犀草和木犀草的叶片有腺毛和非腺毛。一种类型的腺毛被确定:盾状毛。毛状体分布在叶片表面,具有多种功能,在植物、植物器官的位置、密度、形态等方面变化极大,因此其形态和结构在植物中具有重要的分类学意义。腺毛含有或分泌的物质广泛应用于医药、化妆品和农药工业。分布在植物不同器官上的毛状体在结构、形态、密度、分泌和功能上都存在差异。坚果表面可以作为有用的分类学特征。
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引用次数: 0
In Vitro Own-Root Production of Pyrus communis L. cv. Natanz 梨离体自根生产的研究。纳坦兹
Pub Date : 2021-04-05 DOI: 10.22067/JHS.2021.60797.0
G. Davarynejad, S. Karimpour
Introduction Pyrus communis L. cv. Natanz is a popular pear cultivar in Iran because of its customer-friendly attribute due to its excellent characteristics. Pear own-rooted plants has better traits such as high vigorous in growth, low levels on tree losses and damaging by insects (Spornberger et al., 2008; Stanica et al., 2000) rather than cut-rooted and grafted plants. Meristem culture widely used for micropropagation (Erij and Fortes, 2002; Postman and Sugar, 2002), in vitro germplasm preservation (Reed, 1990; Niino and Sakai, 1992; Scottez et al., 1992; Bell and Reed, 2002; Sedlak el al., 2004) and virus eradication purposes in pear (Postman, 1994; Zilka et al., 2002; Dong et al. 2002; Hong et al., 2004; Wang et al., 2006; Postman and Hadidi, 1995; Tan et al. 2010). As pear is belonged to difficult-to-root fruit tree cultivars perhaps the rooting stage is the most important, yet most difficult phase during the in vitro propagation procedure. In vitro rooting of microcuts was varied by genotypes (cultivars) (Sedlak and Paprstein, 2015), type and concentration of used auxin (A1-Maarri el al., 1994; Sedlak and Paprstein, 2015), the method of root induction and formation (Bhojwani et al., 1984; Saadat et al., 2012; Erturk, 2013; Aygun and Dumanglu, 2015), different additional materials such as PVP, polyamines, PP333 (Marino, 1988; Rugini et al., 1992; Erturk, 2013) and so on. Materials and Methods Vegetative buds were taken from current growth shoots of Pyrus communis cv. Natanz from Pear collection orchard (25.36 E, 58.54 N and altitude 1380 meter) of Agricultural and Natural Resources Research and Education Centre of Semnan Province (Shahrood city). New shoots of active buds after 4 weeks transfer to PMI media (Reed et al., 2013) containing BA (0.5, 1, 1.5 mg l-1) and Fe-EDDHA (0, 100, 150 and 200 mg l-1). Meristems (containing 2 newest leaf primordia) was excited from in vitro shoots and incubation on MS media containing BA (0.5, 1, and 1.5 mg l-1) and GA3 (0.1 and 0.5 mg l-1) plus 0.1 mg l-1 IBA for all treatments. Mersitems were kept in dark for 4 days then were transferred to growth chamber. Different concentrations and combinations of two auxins were used. 1000, 2000, 3000 and 4000 mg l-1 of IBA or NAA and two combination solutions of them (1000 IBA+1000 NAA and 2000 IBA+2000 NAA, mg l-1). Shoots were immersing quick dip in solutions for 5 seconds then transfer to PGRs-free PMI medium and kept them to growth chamber. Data of all experiments were analyzed according by completely randomized design (CRD) with 5 replications. BA (3 levels) and Fe-EDDHA (4 levels) for experiment 1; BA (3 levels) and GA3 (2 levels) for experiment 2 were considered as factorial. SAS (v. 9.1) was used for analysis and means were compared with LSD test at 5% probably level. Results and Discussion Proliferated shoot number was affected by BA (p≤0.01) and Fe-EDDHA (p≤0.05) concentrations and also interaction of them (p≤0.05) while BA (p≤0.01) was caused elongation of
黄梨(Pyrus communis L. cv.)纳坦兹是一种受欢迎的梨品种,由于其优良的特性,它的客户友好属性。梨自根植物具有生长旺盛、树木损失少、昆虫危害小等优良特性(Spornberger et al., 2008;Stanica et al., 2000),而不是切根和嫁接植物。分生组织培养广泛用于繁殖(Erij和Fortes, 2002;Postman and Sugar, 2002),体外种质保存(Reed, 1990;Niino和Sakai, 1992;Scottez等人,1992;Bell and Reed, 2002;Sedlak等人,2004)和梨的病毒根除目的(Postman, 1994;Zilka et al., 2002;Dong et al. 2002;Hong et al., 2004;Wang et al., 2006;波兹曼和哈迪迪,1995;Tan et al. 2010)。梨属难根果树品种,在离体繁殖过程中,生根阶段是最重要也是最困难的阶段。微切块的离体生根受不同基因型(品种)(Sedlak and Paprstein, 2015)、使用生长素的类型和浓度(A1-Maarri el al., 1994;Sedlak and Paprstein, 2015),根系诱导和形成方法(Bhojwani et al., 1984;Saadat et al., 2012;Erturk, 2013;Aygun和Dumanglu, 2015),不同的附加材料,如PVP,多胺,PP333 (Marino, 1988;Rugini et al., 1992;Erturk, 2013)等等。材料与方法以黄梨(Pyrus communis cv.)当期生芽为材料,取营养芽。纳坦兹来自塞姆南省(沙赫鲁德市)农业和自然资源研究与教育中心的梨园(东经25.36度,北纬58.54度,海拔1380米)。4周后的新芽转移到含有BA (0.5, 1,1.5 mg l-1)和Fe-EDDHA(0,100, 150和200 mg l-1)的PMI培养基上(Reed et al., 2013)。在含BA(0.5、1和1.5 mg l-1)和GA3(0.1和0.5 mg l-1) + IBA (0.1 mg l-1)的MS培养基上培养,各处理均可诱导出分生组织(含2个最新叶原基)。在黑暗中保存4天,然后转移到生长室。采用两种生长素的不同浓度和组合。1000、2000、3000和4000 mg l-1的IBA或NAA及其两种组合溶液(1000 IBA+1000 NAA和2000 IBA+2000 NAA, mg l-1)。芽在溶液中快速浸渍5秒,然后转移到无pgr的PMI培养基中,保存在生长室中。所有试验数据采用完全随机设计(CRD)进行分析,共5个重复。实验1 BA(3个水平)和Fe-EDDHA(4个水平);实验2的BA(3个水平)和GA3(2个水平)被认为是因子。采用SAS (v. 9.1)进行分析,均数与5%可能水平的LSD检验比较。结果与讨论BA (p≤0.01)和Fe-EDDHA (p≤0.05)浓度对增殖芽数有影响(p≤0.05),BA (p≤0.01)浓度对增殖芽伸长有影响,Fe-EDDHA无影响(p≤0.05)。BA (p≤0.05)、Fe-EDDHA (p≤0.01)浓度和BA×Fe-EDDHA (p≤0.01)互作对叶片产量有显著影响。不同BA浓度下各培养基生长的芽尖均出现坏死现象(p≤0.05)。在我们的实验中,受BA浓度(p≤0.01)、Fe-EDDHA (p≤0.05)和BA×Fe-EDDHA相互作用(p≤0.05)影响的培养基的幂指数计算营养生长。芽在PMI培养基(MS ×1.5 CaCl2)中增殖(5.50芽/外植体)并伸长。2H2O, KH2PO4和MgSO4。BA和Fe-NaEDDHA浓度均较低,成熟叶产量较高。推荐含有1mg l-1 BA + 150mg l-1 Fe-NaEDDHA的PMI培养基用于纳坦兹新梢的增殖,因为增殖后的新梢营养生长最快,质量最高。分别为0.5 mg l-1 BA+ 0.5 mg l-1 GA3(81%)和1 BA mg l-1 + 0.1 mg l-1 GA3(63%)的MS培养基分生组织建立率最高。在添加0.5 mg l-1 BA + 0.5 mg l-1 GA3+0.1 mg l-1 IBA的培养基中,已建立的分生组织自然生长。不同类型的生长素及其浓度对纳坦兹梨品种微梢生根有显著影响(p≤0.05)。NAA在低浓度下诱导生根,IBA随浓度的增加对生根有正向影响。微切口在1000+1000 mg l-1 (IBA+NAA)溶液中快速浸根,然后在PMI培养基中培养。根茎对环境有很好的适应能力。
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引用次数: 0
Physical Properties of Sixteen Indigenous Barberry Genotypes 16个本土小檗基因型的物理特性
Pub Date : 2021-04-05 DOI: 10.22067/JHS.2021.58409.0
M. Khodabandeh, M. Azizi, A. Balandary, H. Aroiee
Introduction: Barberries are a wide class of spiny evergreen or deciduous shrubs belonging to Berberidaceae family that are of great importance due to their nutritional and medicinal properties of their different parts and also their ornamental applications. Genus Berberis, the biggest genus in Berberidaceae, includes more than 660 species. Barberry grows in Asia and Europe; and has been used extensively as a medicinal plant in traditional medicine. In Iranian and Turkish traditional medicine, several properties, such as antibacterial, antipyretic, antipruritic and antiarrhythmic activities have been reported with unknown mechanisms of action. Amazing structural diversity among barberries’ active components makes them a useful source of novel therapeutic compounds. Seedless barberry (Berberis integerrima ‘Asperma’) is one of the Iran’s indigenous valuable medicinal plants. Common asexual propagation of this plant over years and consequently low genetic diversity in populations of the seedless barberry restricts selection outcome in breeding programs. Utilizing the indigenous wild genotypes of barberry genus, which are easily able to cross-pollinate is one of the best methods to increase the genetic diversity. To meet this purpose, several wild seedy barberry genotypes were identified from all over the Iran, collected and established in a collection in Mashhad; then 16 genotypes were selected and their physical properties were studied. Materials and Methods: In this study mature fruits of sixteen unique genotypes (i.e. Iranian seedless barberry and fifteen seedy genotypes) were harvested in October- November, 2015 and kept in refrigerator in order to measure some of their physical properties in fresh fruits (berry dimension, weight of one hundred fresh berries, juice content and color indexes). For other properties, fruits were dried at room temperature. Fruit cluster length was measured by means of a ruler, number of berries/cluster and number of set and aborted seeds/berry by counting, berry dimensions by a digital coulis, weight of one hundred fresh and dried berries, percentage of pulp and seed as well as fruit juice content by a scale with 0.001 accuracy. Moisture content was determined by using an oven with 75 oC temperature for 48 hour. Color indexes including “L”, “a” and “b” were measured by using a chromometer (Model Konica Minolta Chroma Meters CR-410). This study was performed using a completely randomized design with 3 replications. Data were analyzed by Minitab software version 16 using analysis of variance (ANOVA) and differences among means were determined for significance at p
简介:小檗是小檗科的一种多刺的常绿或落叶灌木,由于其不同部位的营养和药用特性以及观赏用途而具有重要意义。小檗属是小檗科中最大的属,包括660多种。小檗生长在亚洲和欧洲;在传统医学中被广泛用作药用植物。在伊朗和土耳其传统医学中,一些特性,如抗菌、解热、止痒和抗心律失常活性,已被报道,但作用机制未知。令人惊叹的结构多样性的有效成分,使其成为新的治疗化合物的有用来源。无籽小檗(Berberis integerrima ' Asperma ')是伊朗本土有价值的药用植物之一。无籽小檗的无性繁殖和遗传多样性低限制了其在育种中的选择结果。利用易于异花授粉的本土野生杨梅属基因型是增加杨梅属遗传多样性的最佳方法之一。为了达到这一目的,从伊朗各地鉴定了几种野生有籽小檗基因型,收集并建立在马什哈德的一个收藏中;然后选择16个基因型,对其物理特性进行研究。材料与方法:本研究于2015年10 - 11月收获16个独特基因型的成熟果实(即伊朗无籽小梅和15个有籽基因型),并将其放入冰箱中保存,以测量其在新鲜水果中的一些物理特性(浆果尺寸、百粒新鲜浆果重量、果汁含量和颜色指标)。对于其他特性,水果在室温下干燥。用直尺测量果簇长度,用计数方法测量果簇数量,用计数方法测量果簇数量,用数字计数方法测量果粒尺寸,用精度为0.001的刻度测量100个新鲜和干燥浆果的重量,果肉和种子的百分比以及果汁含量。在75℃的烘箱中放置48小时来测定水分含量。用色度计(柯尼卡美能达色度计CR-410)测量颜色指数“L”、“a”和“b”。本研究采用完全随机设计,3个重复。数据采用Minitab软件版本16进行方差分析(ANOVA),以p为显著性确定均值之间的差异
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引用次数: 0
Ethnobotanical and on Farm Genetic Surveys of Fig (Ficus carica L.) Genetic Resources in Kerkennah Islands 无花果(Ficus carica L.)的民族植物学和农场遗传调查Kerkennah群岛的遗传资源
Pub Date : 2021-04-01 DOI: 10.22059/IJHST.2020.310191.396
O. Debbabi, Emna Khanfir, M. Dridi, M. Mars
Fig tree (Ficus carica L.) is characterized by a wide genetic diversity in Tunisia although it is considered as a secondary species. In Kerkennah islands, despite several biotic and abiotic stresses, local population has conserved the fig germplasm. This species is very well suited to the harsh environments. In order to enhance on farm conservation of fig genetic resources and to have inventory of related traditional knowledge (TK), a global survey was conducted among a wide range of stakeholders and local governmental agencies in Kerkennah archipelago. For this purpose, prospections were done during two successive years covering the totality of the islands. A total of 9 locations and 26 sub-locations were visited. Twenty four farmers were solicited. Surveys have taken into account sex, age of farmers, predominant agricultural activities of farmers, field area, fig cultivated varieties, land management, traditional knowledge, production and its use, source of incomes and marketing of production. Participatory Four Cell Analysis (FCA) allowed the classification of fig cultivars regarding the threat level of loss and the adequate manner of conservation. Results of this study showed that it is imperative to pay particular attention to threatened and rare cultivars. Hence, the importance to conserve such diversified germplasm. On farm conservation program is a suitable alternative for such region for preservation of traditional knowledge, cultivars rehabilitation and a sustainable agriculture.
无花果树(Ficus carica L.)在突尼斯具有广泛的遗传多样性,尽管它被认为是一种次要物种。在Kerkennah群岛,尽管有一些生物和非生物的压力,当地的种群仍然保存了无花果的种质资源。这个物种非常适应恶劣的环境。为了加强无花果遗传资源的农场保护和相关传统知识的盘点,在Kerkennah群岛广泛的利益相关者和地方政府机构中进行了一项全球调查。为此目的,连续两年对整个岛屿进行了勘探。共访问了9个地点和26个分地点。征集了24个农民。调查考虑到了农民的性别、年龄、农民的主要农业活动、田地面积、无花果栽培品种、土地管理、传统知识、生产及其使用、收入来源和产品销售。参与式四细胞分析(FCA)可以根据损失的威胁程度和适当的保护方式对无花果品种进行分类。研究结果表明,对濒危和珍稀品种的保护是当务之急。因此,保护这种多样化的种质资源具有重要意义。农业保护项目是该地区传统知识保护、品种恢复和农业可持续发展的理想选择。
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引用次数: 3
Effect of Grafting Methods and Dates on the Graft Take Rate of Persian Walnut in Open Field Condition 露天条件下嫁接方式和日期对波斯核桃接枝率的影响
Pub Date : 2021-04-01 DOI: 10.22059/IJHST.2020.311553.401
R. Thapa, Praseed Thapa, K. Ahamad, K. Vahdati
A field-based experiment was carried out in Rukum (East), Nepal, from February to June 2020, to assess the effect of different methods and dates of grafting on the graft take rate of Persian walnut (Juglans regia L.) in field condition. The experiment comprised ten treatments with combination of two factors. The two factors included the grafting dates including: 13th, 19th, and 26th of February, 4th and 11th of March and grafting methods including: outdoor grafting and bench grafting (tongue grafting in both). The experiment was laid out in a Randomized Complete Block Design with three replications. The findings revealed that bench grafting resulted in significantly better outputs than outdoor method for length of scion (at 30 and 60 days after grafting) and graft take rates. Bench grafting showed 44% graft take followed by outdoor grafting (38% graft take). Plants grafted on 13th and 19th of February showed statistically better results for growth performance over the other dates. Plants grafted on 19th of February showed 67% graft take with 53% saleable plants, but statistically similar to the plants grafted on 13th and 26th of February. The earliest bud burst was observed in plants grafted on 13th and 19th of February in 31 and 33 days, respectively. Bench grafting on 19th February showed significantly higher length of scion (16.4 cm) at 60 days after grafting. However, non-significant results were seen for other parameters due to the interaction. Therefore, bench grafting on 13th,19th and 26th of February led to the best graft take in Persian Walnut.Abbreviations: CV: Coefficient of Variation, LSD: Least Significant Difference, ha: hectare, mt: Metric tons, PMAMP: Prime Minister Agriculture Modernization Project, RCBD: Randomized Complete
2020年2月至6月,在尼泊尔鲁库姆(东部)进行了田间试验,研究了不同嫁接方式和嫁接时间对波斯核桃(Juglans regia L.)接枝率的影响。试验采用两因素联合处理,共10个处理。两个因素包括嫁接日期:2月13日、19日、26日,3月4日、11日,嫁接方式:户外嫁接和长凳嫁接(两种方式均采用舌接)。实验采用随机完全区组设计,重复3次。结果表明,在接穗长度(嫁接后30天和60天)和接枝率方面,台阶嫁接的产量明显优于室外嫁接。长凳接枝率为44%,其次是室外接枝率(38%)。2月13日和19日嫁接植株的生长性能优于其他日期。2月19日嫁接植株的接枝率为67%,可售植株为53%,但与2月13日和26日嫁接植株的接枝率无统计学差异。2月13日和19日嫁接植株的芽最早萌发时间分别为31天和33天。2月19日的台地嫁接在嫁接后60 d的接穗长度显著增加(16.4 cm)。然而,由于相互作用,其他参数的结果不显著。因此,2月13日、19日和26日的长凳嫁接是波斯核桃的最佳嫁接时间。缩写:CV:变异系数,LSD:最小显著差异,ha:公顷,mt:公吨,PMAMP:总理农业现代化项目,RCBD:随机完成
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引用次数: 12
Papaya Vivipary: An Unusual Finding of “An Umbilical Cord” 木瓜胎生派对:一个不寻常的“脐带”发现
Pub Date : 2021-04-01 DOI: 10.22059/IJHST.2020.313934.414
A. Garg, Preeti Garg
Vivipary is not a rare finding in papaya fruit. However, this short communication describes the unusual germination of seedling within intra-ovarian ovary. The seedling is attached at two ends of the immature ovary: by radicle at one end, and by elongated tubular structure at the other end. The tubular structure, probably a hypocotyl, mimics an umbilical cord connecting the growing fetus with that of placenta in human embryonic development.
在木瓜果实中,胎生现象并不罕见。然而,这种简短的通信描述了卵巢内卵巢内幼苗的不寻常萌发。幼苗附着在未成熟子房的两端:一端是胚根,另一端是细长的管状结构。管状结构,可能是下胚轴,模仿人类胚胎发育中连接生长中的胎儿和胎盘的脐带。
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引用次数: 1
期刊
Journal of Horticultural Science
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