Journal of King Saud University - Science最新文献

英文中文

Impact of a post-lifestyle modification program in circulating angiopoietin-like proteins in Arab adolescents 生活方式调整后计划对阿拉伯青少年循环血管生成素样蛋白的影响

IF 3.7 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Journal of King Saud University - Science

Pub Date : 2024-09-30 DOI: 10.1016/j.jksus.2024.103469

This study investigated the differences and associations between serum levels of angiopoietin-like (ANGPTL)3, ANGPTL4, and ANGPTL8 in Saudi adolescents with varying levels of obesity and glycemia before and after undergoing a 12-month lifestyle change intervention. A total of 218 Saudi adolescents (45 normal, 77 overweight, and 96 obese) (mean age = 15.1 ± 1.5 years; mean BMI = 35.1 ± 6.6 kg/m²) were included. Anthropometrics were measured at baseline and follow-up. Overnight-fasting serum samples were also collected at baseline and after 12 months of intervention for routine analysis of fasting glucose, glycated hemoglobin (HbA1c) and lipid profile. Serum levels of insulin and ANGPTLs were assessed using commercial assays. At baseline, obese children had a higher ANGPTL8 level [0.7 ng/ml (0.4–1.3), p = 0.03]. In children with prediabetes (defined as those with HbA1c > 5.7), ANGPTL8 levels and HbA1c were significantly lower post-intervention [0.2 (0.1–0.8), p = 0.002; 5.5 ± 1.1), p < 0.001, respectively]. Follow-up lipid profile and insulin levels were significantly higher in all participants with prediabetes and obesity (p-values < 0.05). ANGPTL8 levels of those who achieved 5 % weight loss (N = 25) showed no difference after follow-up (p = 0.59). In conclusion, following a 12-month lifestyle modification program, decreased levels of circulating ANGPTL8 were observed in adolescents with obesity and prediabetes. Consequently, the degree of insulin resistance may be improved by reducing reduced ANGPTL8 levels.

本研究调查了不同肥胖程度的沙特青少年在接受为期 12 个月的改变生活方式干预前后血清中血管生成素样 (ANGPTL)3、ANGPTL4 和 ANGPTL8 水平的差异和关联。共纳入 218 名沙特青少年（45 名正常、77 名超重和 96 名肥胖）（平均年龄 = 15.1 ± 1.5 岁；平均体重指数 = 35.1 ± 6.6 kg/m2）。在基线和随访时测量了人体测量学指标。在基线和干预 12 个月后还收集了隔夜空腹血清样本，用于空腹血糖、糖化血红蛋白 (HbA1c) 和血脂概况的常规分析。血清中的胰岛素和ANGPTLs水平采用商业测定法进行评估。基线时，肥胖儿童的 ANGPTL8 水平较高 [0.7 ng/ml (0.4-1.3)，p = 0.03]。在患有糖尿病前期（定义为 HbA1c 为 5.7）的儿童中，干预后 ANGPTL8 水平和 HbA1c 显著降低 [分别为 0.2 (0.1-0.8), p = 0.002; 5.5 ± 1.1), p < 0.001]。所有患有糖尿病前期和肥胖症的参与者的随访血脂和胰岛素水平均明显升高（p 值为 0.05）。体重减轻 5% 的参与者（25 人）的 ANGPTL8 水平在随访后无差异（p = 0.59）。总之，在实施为期 12 个月的生活方式调整计划后，观察到肥胖和糖尿病前期青少年的循环 ANGPTL8 水平有所下降。因此，胰岛素抵抗的程度可能会通过降低 ANGPTL8 水平而得到改善。

{"title":"Impact of a post-lifestyle modification program in circulating angiopoietin-like proteins in Arab adolescents","authors":"","doi":"10.1016/j.jksus.2024.103469","DOIUrl":"10.1016/j.jksus.2024.103469","url":null,"abstract":"<div><div>This study investigated the differences and associations between serum levels of angiopoietin-like (ANGPTL)3, ANGPTL4, and ANGPTL8 in Saudi adolescents with varying levels of obesity and glycemia before and after undergoing a 12-month lifestyle change intervention. A total of 218 Saudi adolescents (45 normal, 77 overweight, and 96 obese) (mean age = 15.1 ± 1.5 years; mean BMI = 35.1 ± 6.6 kg/m<sup>2</sup>) were included. Anthropometrics were measured at baseline and follow-up. Overnight-fasting serum samples were also collected at baseline and after 12 months of intervention for routine analysis of fasting glucose, glycated hemoglobin (HbA1c) and lipid profile. Serum levels of insulin and ANGPTLs were assessed using commercial assays. At baseline, obese children had a higher ANGPTL8 level [0.7 ng/ml (0.4–1.3), p = 0.03]. In children with prediabetes (defined as those with HbA1c > 5.7), ANGPTL8 levels and HbA1c were significantly lower post-intervention [0.2 (0.1–0.8), p = 0.002; 5.5 ± 1.1), p < 0.001, respectively]. Follow-up lipid profile and insulin levels were significantly higher in all participants with prediabetes and obesity (p-values < 0.05). ANGPTL8 levels of those who achieved 5 % weight loss (N = 25) showed no difference after follow-up (p = 0.59). In conclusion, following a 12-month lifestyle modification program, decreased levels of circulating ANGPTL8 were observed in adolescents with obesity and prediabetes. Consequently, the degree of insulin resistance may be improved by reducing reduced ANGPTL8 levels.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic analyses and pathogenic diversity of Meloidogyne graminicola of rice (cv. BRRI Dhan28) from different agro-ecological zones of Bangladesh 孟加拉国不同农业生态区域水稻（BRRI Dhan28 栽培品种）Meloidogyne graminicola 的系统发育分析和病原多样性

IF 3.7 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Journal of King Saud University - Science

Pub Date : 2024-09-28 DOI: 10.1016/j.jksus.2024.103472

Rice root-knot caused by Meloidogyne graminicola is a major yield-limiting factor in all rice growing countries around the world, which is often ignored in Bangladesh due to its subtle and obscure above ground symptoms. The current study proposes a comprehensive diagnosis of rice root-knot nematode at the molecular level and analyzes the pathogenic diversity of M. graminicola population of different AEZs. Rice seedlings of a popular variety (BRRI Dhan28) showing galls in the roots were collected from thirty Agro-Ecological-Zones (AEZs) of Bangladesh. For molecular identification, nuclear DNA was collected from single second-stage juvenile and internal transcribed spacer (ITS) was used as a universal primer. Molecular identification and phylogenetic analyses revealed that all nematode populations of galled rice seedlings from different locations belong to M. graminicola. The nematode populations constitute six phylogenetic clades. Nematode population of phylogenetic clade II showed the highest no. of galls (178 ± 1.67), although galling index was higher and similar in clade I, clade-II, clade-III and clade-VI. The highest percent reduction in both vegetative and yield-contributing traits were recorded in clade-II. A negative correlation was found in galling incidence and all growth and yield parameters. Comparative hatching of M. graminicola from different locations reveals that the pathogenicity of M. graminicola depends on the genetic variability of the nematode population, but not on the hatched juveniles. It might be concluded that the rice root-knot nematodes (M. graminicola) are prevalent in all agro-ecological zones of Bangladesh, despite the most pathogenic groups being frequent in AEZ- 1, 6, 7, 10, 18, 19, 21 and appropriate management initiatives are therefore crucial for maximizing rice yield.

由禾谷圆线虫（Meloidogyne graminicola）引起的水稻根结线虫是世界上所有水稻种植国的一个主要产量限制因素，在孟加拉国，由于其细微和不明显的地上部症状，往往被忽视。本研究提出了分子水平的水稻根结线虫综合诊断方法，并分析了不同农业经济区禾谷盘线虫种群的致病多样性。从孟加拉国 30 个农业生态区（AEZ）采集了根部出现虫瘿的流行品种（BRRI Dhan28）的稻苗。为进行分子鉴定，从单株第二期幼苗中采集了核 DNA，并使用内部转录间隔（ITS）作为通用引物。分子鉴定和系统进化分析表明，来自不同地点的五倍子水稻幼苗中的所有线虫种群都属于禾本科线虫（M. graminicola）。线虫种群构成了六个系统发育支系。系统发育支系 II 的线虫种群虫瘿数量最多（178 ± 1.67），但支系 I、支系 II、支系 III 和支系 VI 的虫瘿指数较高且相似。支系 II 的无性系和产量贡献性状的减少百分比最高。虫瘿发生率与所有生长和产量参数呈负相关。对不同地点的啮齿目线虫孵化情况进行比较后发现，啮齿目线虫的致病性取决于线虫种群的遗传变异，而不取决于孵化的幼虫。因此，适当的管理措施对最大限度提高水稻产量至关重要。

{"title":"Phylogenetic analyses and pathogenic diversity of Meloidogyne graminicola of rice (cv. BRRI Dhan28) from different agro-ecological zones of Bangladesh","authors":"","doi":"10.1016/j.jksus.2024.103472","DOIUrl":"10.1016/j.jksus.2024.103472","url":null,"abstract":"<div><div>Rice root-knot caused by <em>Meloidogyne graminicola</em> is a major yield-limiting factor in all rice growing countries around the world, which is often ignored in Bangladesh due to its subtle and obscure above ground symptoms. The current study proposes a comprehensive diagnosis of rice root-knot nematode at the molecular level and analyzes the pathogenic diversity of <em>M. graminicola</em> population of different AEZs. Rice seedlings of a popular variety (BRRI Dhan28) showing galls in the roots were collected from thirty Agro-Ecological-Zones (AEZs) of Bangladesh. For molecular identification, nuclear DNA was collected from single second-stage juvenile and internal transcribed spacer (ITS) was used as a universal primer. Molecular identification and phylogenetic analyses revealed that all nematode populations of galled rice seedlings from different locations belong to <em>M. graminicola</em>. The nematode populations constitute six phylogenetic clades. Nematode population of phylogenetic clade II showed the highest no. of galls (178 ± 1.67), although galling index was higher and similar in clade I, clade-II, clade-III and clade-VI. The highest percent reduction in both vegetative and yield-contributing traits were recorded in clade-II. A negative correlation was found in galling incidence and all growth and yield parameters. Comparative hatching of <em>M. graminicola</em> from different locations reveals that the pathogenicity of <em>M. graminicola</em> depends on the genetic variability of the nematode population, but not on the hatched juveniles. It might be concluded that the rice root-knot nematodes (<em>M. graminicola</em>) are prevalent in all agro-ecological zones of Bangladesh, despite the most pathogenic groups being frequent in AEZ- 1, 6, 7, 10, 18, 19, 21 and appropriate management initiatives are therefore crucial for maximizing rice yield.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing early detection of autistic spectrum disorder in children using machine learning approaches 利用机器学习方法加强儿童自闭症谱系障碍的早期检测

IF 3.7 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Journal of King Saud University - Science

Pub Date : 2024-09-27 DOI: 10.1016/j.jksus.2024.103468

Diagnosing Autism Spectrum Disorder (ASD) presents a multifaceted challenge, demanding accurate and efficient screening methods. Applying machine learning techniques offers a promising avenue for enhancing diagnostic accuracy and efficiency. This research investigates the efficiency of machine learning in distinguishing individuals with ASD from those without, utilizing a comprehensive dataset comprising screening questions, demographic factors, and ASD related diagnostic classifications. We applied chi-square feature selection technique and also tested Random Forest, Logistic Regression, Gradient Boosting Classifier, and Extra Trees Classifier. Each model showed optimal performance and exhibit high precision, recall, and F1-score for both ASD-positive and ASD-negative instances. Additionally, AUROC curves further validated the models’ exceptional discriminatory abilities, with exceptional results. Our findings highlight the potential of machine learning algorithms for enhancing ASD diagnosis accuracy and efficiency in clinical settings. Further research and validation on larger datasets are required to understand the importance of machine learning methods in ASD diagnosis.

自闭症谱系障碍（ASD）的诊断是一项多方面的挑战，需要准确高效的筛查方法。应用机器学习技术为提高诊断的准确性和效率提供了一条前景广阔的途径。本研究利用由筛查问题、人口统计因素和 ASD 相关诊断分类组成的综合数据集，研究机器学习在区分 ASD 患者和非 ASD 患者方面的效率。我们应用了卡方特征选择技术，还测试了随机森林、逻辑回归、梯度提升分类器和额外树分类器。每个模型都显示出最佳性能，在 ASD 阳性和 ASD 阴性实例中都表现出较高的精确度、召回率和 F1 分数。此外，AUROC 曲线进一步验证了这些模型卓越的判别能力和优异的结果。我们的研究结果凸显了机器学习算法在临床环境中提高 ASD 诊断准确性和效率的潜力。要了解机器学习方法在 ASD 诊断中的重要性，还需要在更大的数据集上进行进一步的研究和验证。

{"title":"Enhancing early detection of autistic spectrum disorder in children using machine learning approaches","authors":"","doi":"10.1016/j.jksus.2024.103468","DOIUrl":"10.1016/j.jksus.2024.103468","url":null,"abstract":"<div><div>Diagnosing Autism Spectrum Disorder (ASD) presents a multifaceted challenge, demanding accurate and efficient screening methods. Applying machine learning techniques offers a promising avenue for enhancing diagnostic accuracy and efficiency. This research investigates the efficiency of machine learning in distinguishing individuals with ASD from those without, utilizing a comprehensive dataset comprising screening questions, demographic factors, and ASD related diagnostic classifications. We applied chi-square feature selection technique and also tested Random Forest, Logistic Regression, Gradient Boosting Classifier, and Extra Trees Classifier. Each model showed optimal performance and exhibit high precision, recall, and F1-score for both ASD-positive and ASD-negative instances. Additionally, AUROC curves further validated the models’ exceptional discriminatory abilities, with exceptional results. Our findings highlight the potential of machine learning algorithms for enhancing ASD diagnosis accuracy and efficiency in clinical settings. Further research and validation on larger datasets are required to understand the importance of machine learning methods in ASD diagnosis.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “A synthetic antioxidant molecule, GP13 derived from cysteine desulfurase of spirulina, Arthrospira platensis exhibited anti-diabetic activity on L6 rat skeletal muscle cells through GLUT-4 pathway” [J. King Saud Univ. – Sci. 35(2) (2023) 102450] 对 "螺旋藻半胱氨酸脱硫酶合成的抗氧化分子 GP13 通过 GLUT-4 通路对 L6 大鼠骨骼肌细胞表现出抗糖尿病活性"[J. King Saud Univ.

IF 3.7 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Journal of King Saud University - Science

Pub Date : 2024-09-27 DOI: 10.1016/j.jksus.2024.103460

引用次数: 0

Impact of olive oil and potassium carbonate pretreatment on physico-chemical properties of sun-dried Sundar Khani raisins (Vitis vinifera L.) 橄榄油和碳酸钾预处理对晒干的 Sundar Khani 葡萄干（葡萄属）理化特性的影响

IF 3.7 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Journal of King Saud University - Science

Pub Date : 2024-09-27 DOI: 10.1016/j.jksus.2024.103465

Sundar Khani is native grape variety of Pakistan which has long berry length and significantly rich in taste and sweetness. There is limited literature on pretreatment and drying data for this valuable variety of Grapes. Current study was conducted to investigate the physio-chemical indices of potassium carbonate (K₂CO₃) and olive oil combined treatments followed by natural sun drying method. The effects of 2 % K₂CO₃ pretreatments alone and in combination with various amounts of pure olive oil (1 %, 2 %, and 3 %) were studied. The results showed that, physical attributes like raisin length, width, volume, weight and total replication weight increased under distilled water treatment, while, combination of 2 % K₂CO₃ + 3 % Olive oil significantly reduced the drying time, 2 % K₂CO₃ + 1 % Olive oil gave higher final yield, rehydration ratio was highest under dH₂O treatment and greater decay loss was observed at 2 %K₂CO₃ + 2 % olive oil. Further, 2 % K₂CO₃ displayed higher ratios of total soluble solids, sugars and peroxidase enzyme activity as well as titratable acidity, anthocyanins, total phenols, flavonoids were maximum at 2 % K₂CO₃ + 2 % Olive oil pretreatment. In addition, 2 % K₂CO₃ + 3 % Olive oil increased the Catalase enzyme activity as well as achieved higher scores in the evaluation of sensory attributes particularly morphological form, color and overall acceptance. This study aimed to investigate the physicochemical indices of Sundar Khani grapes under different pretreatment and sun-drying conditions using K₂CO₃ and olive oil and it could be suggested that raisin production of Sundar Khani by using above mentioned treatments along with sun drying could have future scope both in export and industrial markets due to its incredible reduction in drying and processing time.

Sundar Khani 是巴基斯坦的本土葡萄品种，浆果长，口感丰富，甜度高。有关这一珍贵葡萄品种的预处理和干燥数据的文献资料十分有限。本研究旨在调查碳酸钾（K2CO3）和橄榄油混合处理后自然晒干法的物理化学指标。研究了 2 % K2CO3 单独预处理和与不同量的纯橄榄油（1 %、2 % 和 3 %）混合预处理的效果。结果表明，在蒸馏水处理下，葡萄干的长度、宽度、体积、重量和总重量等物理属性都有所增加，而 2 % K2CO3 + 3 % 橄榄油的组合则大大缩短了干燥时间，2 % K2CO3 + 1 % 橄榄油的最终产量更高，dH2O 处理下的再水化率最高，2 % K2CO3 + 2 % 橄榄油处理下的腐烂损失更大。此外，在 2 %K2CO3 + 2 % 橄榄油预处理中，2 %K2CO3 显示出更高的总可溶性固体、糖和过氧化物酶活性比率，可滴定酸度、花青素、总酚、黄酮类化合物也最高。此外，2 % K2CO3 + 3 % 橄榄油还能提高过氧化氢酶的活性，并在感官属性评估中获得更高的分数，特别是形态、颜色和总体接受度。本研究旨在调查在不同预处理和使用 K2CO3 和橄榄油进行日晒干燥条件下，Sundar Khani 葡萄的理化指标。

{"title":"Impact of olive oil and potassium carbonate pretreatment on physico-chemical properties of sun-dried Sundar Khani raisins (Vitis vinifera L.)","authors":"","doi":"10.1016/j.jksus.2024.103465","DOIUrl":"10.1016/j.jksus.2024.103465","url":null,"abstract":"<div><div>Sundar Khani is native grape variety of Pakistan which has long berry length and significantly rich in taste and sweetness. There is limited literature on pretreatment and drying data for this valuable variety of Grapes. Current study was conducted to investigate the physio-chemical indices of potassium carbonate (K<sub>2</sub>CO<sub>3</sub>) and olive oil combined treatments followed by natural sun drying method. The effects of 2 % K<sub>2</sub>CO<sub>3</sub> pretreatments alone and in combination with various amounts of pure olive oil (1 %, 2 %, and 3 %) were studied. The results showed that, physical attributes like raisin length, width, volume, weight and total replication weight increased under distilled water treatment, while, combination of 2 % K<sub>2</sub>CO<sub>3</sub> + 3 % Olive oil significantly reduced the drying time, 2 % K<sub>2</sub>CO<sub>3</sub> + 1 % Olive oil gave higher final yield, rehydration ratio was highest under dH<sub>2</sub>O treatment and greater decay loss was observed at 2 %K<sub>2</sub>CO<sub>3</sub> + 2 % olive oil. Further, 2 % K<sub>2</sub>CO<sub>3</sub> displayed higher ratios of total soluble solids, sugars and peroxidase enzyme activity as well as titratable acidity, anthocyanins, total phenols, flavonoids were maximum at 2 % K<sub>2</sub>CO<sub>3</sub> + 2 % Olive oil pretreatment. In addition, 2 % K<sub>2</sub>CO<sub>3</sub> + 3 % Olive oil increased the Catalase enzyme activity as well as achieved higher scores in the evaluation of sensory attributes particularly morphological form, color and overall acceptance. This study aimed to investigate the physicochemical indices of Sundar Khani grapes under different pretreatment and sun-drying conditions using K<sub>2</sub>CO<sub>3</sub> and olive oil and it could be suggested that raisin production of Sundar Khani by using above mentioned treatments along with sun drying could have future scope both in export and industrial markets due to its incredible reduction in drying and processing time.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial Efficacy of AgNPs synthesized from Aloe vera extract and Staphylococcus aureus Culture Supernatant 芦荟提取物和金黄色葡萄球菌培养上清液合成的 AgNPs 的抗菌功效

IF 3.7 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Journal of King Saud University - Science

Pub Date : 2024-09-26 DOI: 10.1016/j.jksus.2024.103464

Biological synthesis of silver nanoparticles (AgNPs) is an emerging method that avoids the need for costly equipment and hazardous chemicals. Therefore, in this study, Aloe vera (AV) extract and culture supernatant of Staphylococcus aureus (CS-S. aureus) were used to reduce and stabilise silver nanoparticles. The NPs were characterized using UV–vis spectrophotometry at 445 nm for AV-AgNP and 444.5 nm for CS-S.aureus AgNP, and the presence of silver was confirmed by energy dispersive X-ray (EDX). Furthermore, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that the AV-CS of S. aureus-AgNPs had irregular and spherical shapes, with average sizes of 18.5 nm for AvAgNPs and 7.03 nm for CS-S. aureus-AgNPs. Additionally, the minimum inhibitory concentration (MIC) of both AgNPs showed a similar reaction. K. pneumoniae, P. aeruginosa, and S. epidermidis were more sensitive to the AgNPs compared to methicillin-resistant Staphylococcus aureus (MRSA). In conclusion, our study used a green method to synthesise silver nanoparticles using AV extract and CS of S. aureus. The NPs showed a remarkable antibacterial effect, especially CS-S. aureus-AgNP, which could have application as a treatment against both Gram-positive and Gram-negative bacteria.

生物合成银纳米粒子（AgNPs）是一种新兴的方法，可避免使用昂贵的设备和危险化学品。因此，在本研究中，芦荟（AV）提取物和金黄色葡萄球菌（CS-S. aureus）培养上清液被用来还原和稳定银纳米粒子。使用紫外-可见分光光度法在 445 纳米波长对 AV-AgNP 和 444.5 纳米波长对 CS-S.aureus AgNP 进行了表征，并通过能量色散 X 射线（EDX）确认了银的存在。此外，扫描电子显微镜（SEM）和透射电子显微镜（TEM）显示，金黄色葡萄球菌-AgNPs 的 AV-CS 呈不规则球形，AvAgNPs 的平均尺寸为 18.5 nm，CS-S.aureus-AgNPs 的平均尺寸为 7.03 nm。此外，两种 AgNPs 的最小抑菌浓度（MIC）显示出相似的反应。与耐甲氧西林金黄色葡萄球菌（MRSA）相比，肺炎双球菌、绿脓杆菌和表皮葡萄球菌对 AgNPs 更为敏感。总之，我们的研究采用绿色方法，利用金黄色葡萄球菌的 AV 提取物和 CS 合成了银纳米粒子。银纳米粒子显示出显著的抗菌效果，尤其是 CS-S. aureus-AgNP，可用于治疗革兰氏阳性和革兰氏阴性细菌。

{"title":"Antibacterial Efficacy of AgNPs synthesized from Aloe vera extract and Staphylococcus aureus Culture Supernatant","authors":"","doi":"10.1016/j.jksus.2024.103464","DOIUrl":"10.1016/j.jksus.2024.103464","url":null,"abstract":"<div><div>Biological synthesis of silver nanoparticles (AgNPs) is an emerging method that avoids the need for costly equipment and hazardous chemicals. Therefore, in this study, <em>Aloe vera</em> (<em>AV</em>) extract and culture supernatant of <em>Staphylococcus aureus</em> (CS-<em>S. aureus</em>) were used to reduce and stabilise silver nanoparticles. The NPs were characterized using UV–vis spectrophotometry at 445 nm for <em>AV</em>-AgNP and 444.5 nm for CS-<em>S.aureus</em> AgNP, and the presence of silver was confirmed by energy dispersive X-ray (EDX). Furthermore, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that the <em>AV</em>-CS of <em>S. aureus</em>-AgNPs had irregular and spherical shapes, with average sizes of 18.5 nm for <em>AvAgNPs</em> and 7.03 nm for CS-<em>S</em>. <em>aureus</em>-AgNPs. Additionally, the minimum inhibitory concentration (MIC) of both AgNPs showed a similar reaction. <em>K. pneumoniae</em>, <em>P. aeruginosa</em>, and <em>S. epidermidis</em> were more sensitive to the AgNPs compared to methicillin-resistant <em>Staphylococcus aureus</em> (MRSA). In conclusion, our study used a green method to synthesise silver nanoparticles using <em>AV</em> extract and CS of <em>S. aureus</em>. The NPs showed a remarkable antibacterial effect, especially CS-<em>S. aureus-</em>AgNP, which could have application as a treatment against both Gram-positive and Gram-negative bacteria.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly synthesis of nano ferrites for effective dye degradation and enhanced antimicrobial protection 以生态友好的方式合成纳米铁氧体，有效降解染料并增强抗菌保护作用

IF 3.7 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Journal of King Saud University - Science

Pub Date : 2024-09-26 DOI: 10.1016/j.jksus.2024.103466

This study investigates the synthesis of cobalt and nickel ferrite NPs using Araucaria heterophylla resin as a bio- template and nitrate salts of cobalt, nickel, and iron as precursors.The resulting spinel systems exhibit efficient degradation of organic dyes, including methylene blue and rhodamine-B, and demonstrate excellent reusability. The NPs antibacterial efficacy against pathogenic bacteria, such as Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Salmonella paratyphi, was also evaluated. Comprehensive characterization of the nanomaterials was performed using FT-IR, XRD, SEM, TEM, EDX, and VSM techniques. Magnetic measurements revealed the ferromagnetic nature of cobalt ferrites and the superparamagnetic nature of nickel ferrites, with reduced saturation magnetization and coercivity compared to bulk materials due to the quantum size effect. The nano-formulated ferrites exhibited superior antibacterial activity, highlighting their potential for water treatment applications. This study underscores the importance of removing dye contaminants from wastewater before discharge into aquatic ecosystems.

本研究以Araucaria heterophylla树脂为生物模板，以钴、镍和铁的硝酸盐为前驱体，研究了钴和镍铁氧体NPs的合成。此外，还评估了 NPs 对金黄色葡萄球菌、枯草杆菌、大肠杆菌和副伤寒沙门氏菌等病原菌的抗菌效果。利用傅立叶变换红外光谱（FT-IR）、X 射线衍射（XRD）、扫描电子显微镜（SEM）、电子显微镜（TEM）、电离辐射分析（EDX）和真空吸附（VSM）技术对纳米材料进行了综合表征。磁性测量结果表明，钴铁氧体具有铁磁性，镍铁氧体具有超顺磁性，由于量子尺寸效应，饱和磁化率和矫顽力与块状材料相比有所降低。纳米配方铁氧体表现出卓越的抗菌活性，突出了其在水处理应用方面的潜力。这项研究强调了在废水排入水生生态系统之前去除废水中染料污染物的重要性。

{"title":"Eco-friendly synthesis of nano ferrites for effective dye degradation and enhanced antimicrobial protection","authors":"","doi":"10.1016/j.jksus.2024.103466","DOIUrl":"10.1016/j.jksus.2024.103466","url":null,"abstract":"<div><div>This study investigates the synthesis of cobalt and nickel ferrite NPs using <em>Araucaria heterophylla</em> resin as a bio- template and nitrate salts of cobalt, nickel, and iron as precursors.The resulting spinel systems exhibit efficient degradation of organic dyes, including methylene blue and rhodamine-B, and demonstrate excellent reusability. The NPs antibacterial efficacy against pathogenic bacteria, such as <em>Staphylococcus aureus</em>, <em>Bacillus subtilis</em>, <em>Escherichia coli</em>, and <em>Salmonella paratyphi</em>, was also evaluated. Comprehensive characterization of the nanomaterials was performed using FT-IR, XRD, SEM, TEM, EDX, and VSM techniques. Magnetic measurements revealed the ferromagnetic nature of cobalt ferrites and the superparamagnetic nature of nickel ferrites, with reduced saturation magnetization and coercivity compared to bulk materials due to the quantum size effect. The nano-formulated ferrites exhibited superior antibacterial activity, highlighting their potential for water treatment applications. This study underscores the importance of removing dye contaminants from wastewater before discharge into aquatic ecosystems.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effects of cuminaldehyde on human liver cytochrome P450 enzymes 积甲醛对人体肝脏细胞色素 P450 酶的抑制作用

IF 3.7 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Journal of King Saud University - Science

Pub Date : 2024-09-26 DOI: 10.1016/j.jksus.2024.103467

This work explored the influence of cuminaldehyde on the metabolic activity of four primary human cytochrome P450 (CYP) enzymes. Human liver microsomes (HLM) with and without cuminaldehyde were incubated with specific substrates of different CYP enzymes. The influence of cuminaldehyde on “CYP1A2, CYP2C9, CYP2D6 and CYP3A4” activities was examined. The formation of specific metabolites was analyzed by HPLC analytical methods. It was observed that cuminaldehyde appears to have a negligible influence on CYP1A2 activity. However, CYP2C9 activity was significantly and potently inhibited by cuminaldehyde at all investigated concentrations. It was found that cuminaldehyde (1 µM) inhibited the most CYP2C9 enzyme activity with 64.87 % inhibition followed by CYP3A4 (38.90 %), CYP2D6 (25.76 %) and CYP1A2 (4.99 %). Inhibition of both CYP2D6 and CYP3A4 enzyme activity was observed in response to cuminaldehyde concentrations. Findings of the current investigation indicate that herb-drug interactions are very possible when cuminaldehyde is concurrently taken with medicines predominantly metabolized by CYP2C9 and CYP3A4. Cuminaldehyde should be tested further to examine how it affects CYP2C9 and CYP3A4-metabolized drugs.

这项研究探讨了积甲醛对四种主要人体细胞色素 P450（CYP）酶代谢活性的影响。将含有和未含有积甲醛的人肝微粒体（HLM）与不同 CYP 酶的特定底物进行孵育。研究了积甲醛对 "CYP1A2、CYP2C9、CYP2D6 和 CYP3A4 "活性的影响。采用高效液相色谱分析方法分析了特定代谢物的形成。结果表明，积甲醛对 CYP1A2 活性的影响微乎其微。然而，在所有研究浓度下，积雪醛对 CYP2C9 的活性都有明显而有效的抑制作用。研究发现，积甲醛（1 µM）对 CYP2C9 酶活性的抑制率最高，为 64.87%，其次是 CYP3A4（38.90%）、CYP2D6（25.76%）和 CYP1A2（4.99%）。在积雪草醛浓度的作用下，CYP2D6 和 CYP3A4 的酶活性都受到了抑制。目前的研究结果表明，当积雪草醛与主要由 CYP2C9 和 CYP3A4 代谢的药物同时服用时，很可能会发生草药与药物之间的相互作用。应进一步检测积雪草醛对 CYP2C9 和 CYP3A4 代谢药物的影响。

{"title":"Inhibitory effects of cuminaldehyde on human liver cytochrome P450 enzymes","authors":"","doi":"10.1016/j.jksus.2024.103467","DOIUrl":"10.1016/j.jksus.2024.103467","url":null,"abstract":"<div><div>This work explored the influence of cuminaldehyde on the metabolic activity of four primary human cytochrome P450 (CYP) enzymes. Human liver microsomes (HLM) with and without cuminaldehyde were incubated with specific substrates of different CYP enzymes. The influence of cuminaldehyde on “CYP1A2, CYP2C9, CYP2D6 and CYP3A4” activities was examined. The formation of specific metabolites was analyzed by HPLC analytical methods. It was observed that cuminaldehyde appears to have a negligible influence on CYP1A2 activity. However, CYP2C9 activity was significantly and potently inhibited by cuminaldehyde at all investigated concentrations. It was found that cuminaldehyde (1 µM) inhibited the most CYP2C9 enzyme activity with 64.87 % inhibition followed by CYP3A4 (38.90 %), CYP2D6 (25.76 %) and CYP1A2 (4.99 %). Inhibition of both CYP2D6 and CYP3A4 enzyme activity was observed in response to cuminaldehyde concentrations. Findings of the current investigation indicate that herb-drug interactions are very possible when cuminaldehyde is concurrently taken with medicines predominantly metabolized by CYP2C9 and CYP3A4. Cuminaldehyde should be tested further to examine how it affects CYP2C9 and CYP3A4-metabolized drugs.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142328233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Antibiofilm efficacy of novel biogenic silver nanoparticles from Terminalia catappa against food-borne Listeria monocytogenes ATCC 15,313 and mechanisms investigation in-vivo and in-vitro” [J. King Saud Univ. – Sci. 34(5) (2022) 102083] 沙特国王大学--科学》34(5) (2022) 102083]的更正

IF 3.7 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Journal of King Saud University - Science

Pub Date : 2024-09-25 DOI: 10.1016/j.jksus.2024.103459

引用次数: 0

Corrigendum to “Neuroprotective and antioxidant capability of RW20 peptide from histone acetyltransferases caused by oxidative stress-induced neurotoxicity in in vivo zebrafish larval model” [J. King Saud Univ. – Sci. 34(3) (2022) 101861] 对 "在体内斑马鱼幼体模型中，组蛋白乙酰转移酶引起的氧化应激诱导的神经毒性对 RW20 肽的神经保护和抗氧化能力 "的更正[J. Saud 国王大学-科学. 34(3) (2022) 101861]

IF 3.7 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Journal of King Saud University - Science

Pub Date : 2024-09-25 DOI: 10.1016/j.jksus.2024.103458

引用次数: 0

首页上一页

下一页尾页

类型

全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

数据库

全部 ACS Publications Elsevier ieeexplore Springer The Royal Society of Chemistry Wiley

期刊

Journal of King Saud University - Science

全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.

﹀