Pub Date : 2023-05-15DOI: 10.5614/j.math.fund.sci.2022.54.3.3
O. Cahyonugroho, S. Hariyanto, G. Supriyanto
The relationship between phytoplankton dynamics and dissolved organic matter (DOM) in aquatic systems based on fluorophore organic matter properties results in poor implementation of methods for water quality monitoring. This study aimed to investigate the relation between fluorophore organic matter and phytoplankton index and to estimate the phytoplankton index by fluorescent organic components for water quality assessment. The research was conducted by sampling urban river water, followed by measuring the biological phytoplankton index and dissolved organic matter during the wet and dry seasons. Fluorescence Excitation Emission Matrices (FEEMs) analysis combined with Parallel Factor (PARAFAC) analysis identified three main components of the fluorescence organic matter, i.e., a fulvic acid-like component (C1) and humic acid-like components (C2 and C3), in the aquatic system, whether in the dry season or the wet season. C1 was mainly generated by phytoplankton species, i.e., Nitzschia sp., Plectonema sp., Navicula sp., and Pinnularia sp., and C2 and C3 had a less significant relationship with phytoplankton species. The diversity of Bacillariophyta had a stronger contribution to the release of DOM than the diversity of Cyanobacteria. The abundance index of a particular phytoplankton species can be used to determine the organic components of FEEM-derived fluorescence organic matter.
{"title":"Applying Fluorescence Dissolved Organic Matter Spectra and Phytoplankton Biology Index for Assessing Urban River Quality","authors":"O. Cahyonugroho, S. Hariyanto, G. Supriyanto","doi":"10.5614/j.math.fund.sci.2022.54.3.3","DOIUrl":"https://doi.org/10.5614/j.math.fund.sci.2022.54.3.3","url":null,"abstract":"The relationship between phytoplankton dynamics and dissolved organic matter (DOM) in aquatic systems based on fluorophore organic matter properties results in poor implementation of methods for water quality monitoring. This study aimed to investigate the relation between fluorophore organic matter and phytoplankton index and to estimate the phytoplankton index by fluorescent organic components for water quality assessment. The research was conducted by sampling urban river water, followed by measuring the biological phytoplankton index and dissolved organic matter during the wet and dry seasons. Fluorescence Excitation Emission Matrices (FEEMs) analysis combined with Parallel Factor (PARAFAC) analysis identified three main components of the fluorescence organic matter, i.e., a fulvic acid-like component (C1) and humic acid-like components (C2 and C3), in the aquatic system, whether in the dry season or the wet season. C1 was mainly generated by phytoplankton species, i.e., Nitzschia sp., Plectonema sp., Navicula sp., and Pinnularia sp., and C2 and C3 had a less significant relationship with phytoplankton species. The diversity of Bacillariophyta had a stronger contribution to the release of DOM than the diversity of Cyanobacteria. The abundance index of a particular phytoplankton species can be used to determine the organic components of FEEM-derived fluorescence organic matter.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"65 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72544608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-15DOI: 10.5614/j.math.fund.sci.2023.54.3.2
Dudi Runadi, Purnama Purnama, Supriatno Salam, Nulelasari Nurlelasari, Desi Harneti, Tri Mayanti, Rani Maharani, Unang Supratman, Yoshihito Shiono
A new limonoid, belonging to the havanensin-type, called pentandricine E (1), alongside four other known types, i.e., trichilenone acetate (2), toonaciliatones C (3), 11α-acetoxyazadirone (4) and ceramicine I (5), were found in Chisocheton pentandrus stem bark. The structures of the isolated 1-5 were identified by NMR spectroscopy and HRTOFMS. Pentandricine E (1) displayed the highest cytotoxicity toward the MCF-7 breast cancer line with an IC50 value of 63.27 ± 0.21 mM.
{"title":"Havanensin-type Limonoids from the Stem Bark of Chisocheton pentandrus (Meliaceae)","authors":"Dudi Runadi, Purnama Purnama, Supriatno Salam, Nulelasari Nurlelasari, Desi Harneti, Tri Mayanti, Rani Maharani, Unang Supratman, Yoshihito Shiono","doi":"10.5614/j.math.fund.sci.2023.54.3.2","DOIUrl":"https://doi.org/10.5614/j.math.fund.sci.2023.54.3.2","url":null,"abstract":"A new limonoid, belonging to the havanensin-type, called pentandricine E (1), alongside four other known types, i.e., trichilenone acetate (2), toonaciliatones C (3), 11α-acetoxyazadirone (4) and ceramicine I (5), were found in Chisocheton pentandrus stem bark. The structures of the isolated 1-5 were identified by NMR spectroscopy and HRTOFMS. Pentandricine E (1) displayed the highest cytotoxicity toward the MCF-7 breast cancer line with an IC50 value of 63.27 ± 0.21 mM.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135086143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-15DOI: 10.5614/j.math.fund.sci.2022.54.3.2
U. Supratman, Dudi Runadi, Purnama Purnama, S. Salam, Nulelasari Nurlelasari, D. Harneti, T. Mayanti, R. Maharani, Y. Shiono
A new limonoid, belonging to the havanensin-type, called pentandricine E (1), alongside four other known types, i.e., trichilenone acetate (2), toonaciliatones C (3), 11α-acetoxyazadirone (4) and ceramicine I (5), were found in Chisocheton pentandrus stem bark. The structures of the isolated 1-5 were identified by NMR spectroscopy and HRTOFMS. Pentandricine E (1) displayed the highest cytotoxicity toward the MCF-7 breast cancer line with an IC50 value of 63.27 ± 0.21 mM.
{"title":"Havanensin-type Limonoids from the Stem Bark of Chisocheton pentandrus (Meliaceae)","authors":"U. Supratman, Dudi Runadi, Purnama Purnama, S. Salam, Nulelasari Nurlelasari, D. Harneti, T. Mayanti, R. Maharani, Y. Shiono","doi":"10.5614/j.math.fund.sci.2022.54.3.2","DOIUrl":"https://doi.org/10.5614/j.math.fund.sci.2022.54.3.2","url":null,"abstract":"A new limonoid, belonging to the havanensin-type, called pentandricine E (1), alongside four other known types, i.e., trichilenone acetate (2), toonaciliatones C (3), 11α-acetoxyazadirone (4) and ceramicine I (5), were found in Chisocheton pentandrus stem bark. The structures of the isolated 1-5 were identified by NMR spectroscopy and HRTOFMS. Pentandricine E (1) displayed the highest cytotoxicity toward the MCF-7 breast cancer line with an IC50 value of 63.27 ± 0.21 mM.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"13 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91274187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-07DOI: 10.5614/j.math.fund.sci.2023.54.3.1
Meutia Diva Hakim, Noriko Yamano- Adachi, Takeshi Omasa, Marselina Irasonia Tan, Lia Dewi Juliawaty, Ernawati Arifin Giri-Rachman
Hepatitis B immunoglobulin (HBIG) is an effective treatment for hepatitis B, including postexposure prophylaxis of HBV infection, prevention of HBV reinfection in liver transplant patients, and reducing sexual transmission. This study investigated the synthesis of human IgG antibodies that specifically target HBsAg subtype adr in CHL-YN cells, a newly established cell line that grows faster than CHO-K1. To achieve the synthesis of human IgG antibodies, a plasmid vector encoding DNA sequences for human IgG antibodies against HBsAg was constructed and then transiently transfected into CHL-YN cells. The expression and antigen-binding capacity of the recombinant human IgG antibodies were analyzed using western blot and ELISA. The results showed successful expression and secretion of human IgG antibodies that recognize HBsAg subtype adr in CHL-YN cells. The ELISA test confirmed the specificity of the human IgG antibodies towards HBsAg subtype adr. Thus, this study concluded that human IgG antibodies that target HBsAg subtype adr were transiently expressed in CHL-YN cells.
{"title":"Synthesis of Human Antibodies Against HBsAg in Newly Established Chinese Hamster Lung (CHL-YN) Cell Line","authors":"Meutia Diva Hakim, Noriko Yamano- Adachi, Takeshi Omasa, Marselina Irasonia Tan, Lia Dewi Juliawaty, Ernawati Arifin Giri-Rachman","doi":"10.5614/j.math.fund.sci.2023.54.3.1","DOIUrl":"https://doi.org/10.5614/j.math.fund.sci.2023.54.3.1","url":null,"abstract":"Hepatitis B immunoglobulin (HBIG) is an effective treatment for hepatitis B, including postexposure prophylaxis of HBV infection, prevention of HBV reinfection in liver transplant patients, and reducing sexual transmission. This study investigated the synthesis of human IgG antibodies that specifically target HBsAg subtype adr in CHL-YN cells, a newly established cell line that grows faster than CHO-K1. To achieve the synthesis of human IgG antibodies, a plasmid vector encoding DNA sequences for human IgG antibodies against HBsAg was constructed and then transiently transfected into CHL-YN cells. The expression and antigen-binding capacity of the recombinant human IgG antibodies were analyzed using western blot and ELISA. The results showed successful expression and secretion of human IgG antibodies that recognize HBsAg subtype adr in CHL-YN cells. The ELISA test confirmed the specificity of the human IgG antibodies towards HBsAg subtype adr. Thus, this study concluded that human IgG antibodies that target HBsAg subtype adr were transiently expressed in CHL-YN cells.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135962101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-07DOI: 10.5614/j.math.fund.sci.2022.54.3.1
Meutia Diva Hakim, Noriko Yamano- Adachi, T. Omasa, M. Tan, L. D. Juliawaty, E. Giri-Rachman
Hepatitis B immunoglobulin (HBIG) is an effective treatment for hepatitis B, including postexposure prophylaxis of HBV infection, prevention of HBV reinfection in liver transplant patients, and reducing sexual transmission. This study investigated the synthesis of human IgG antibodies that specifically target HBsAg subtype adr in CHL-YN cells, a newly established cell line that grows faster than CHO-K1. To achieve the synthesis of human IgG antibodies, a plasmid vector encoding DNA sequences for human IgG antibodies against HBsAg was constructed and then transiently transfected into CHL-YN cells. The expression and antigen-binding capacity of the recombinant human IgG antibodies were analyzed using western blot and ELISA. The results showed successful expression and secretion of human IgG antibodies that recognize HBsAg subtype adr in CHL-YN cells. The ELISA test confirmed the specificity of the human IgG antibodies towards HBsAg subtype adr. Thus, this study concluded that human IgG antibodies that target HBsAg subtype adr were transiently expressed in CHL-YN cells.
{"title":"Synthesis of Human Antibodies Against HBsAg in Newly Established Chinese Hamster Lung (CHL-YN) Cell Line","authors":"Meutia Diva Hakim, Noriko Yamano- Adachi, T. Omasa, M. Tan, L. D. Juliawaty, E. Giri-Rachman","doi":"10.5614/j.math.fund.sci.2022.54.3.1","DOIUrl":"https://doi.org/10.5614/j.math.fund.sci.2022.54.3.1","url":null,"abstract":"Hepatitis B immunoglobulin (HBIG) is an effective treatment for hepatitis B, including postexposure prophylaxis of HBV infection, prevention of HBV reinfection in liver transplant patients, and reducing sexual transmission. This study investigated the synthesis of human IgG antibodies that specifically target HBsAg subtype adr in CHL-YN cells, a newly established cell line that grows faster than CHO-K1. To achieve the synthesis of human IgG antibodies, a plasmid vector encoding DNA sequences for human IgG antibodies against HBsAg was constructed and then transiently transfected into CHL-YN cells. The expression and antigen-binding capacity of the recombinant human IgG antibodies were analyzed using western blot and ELISA. The results showed successful expression and secretion of human IgG antibodies that recognize HBsAg subtype adr in CHL-YN cells. The ELISA test confirmed the specificity of the human IgG antibodies towards HBsAg subtype adr. Thus, this study concluded that human IgG antibodies that target HBsAg subtype adr were transiently expressed in CHL-YN cells.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"34 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78293281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.5614/j.math.fund.sci.2022.54.2.5
M. Budiman, Efraim Partogi, A. Kristi, Prianka Anggara, N. Aminah
Population growth causes the demand for food to increase. One solution that can be applied is agriculture with hydroponic technology. To increase production efficiency, one must know the physical parameters that most influence the production process. This research used an IoT system to gather accurate and precise measurement data of physical parameters to be used as a dataset for machine learning. The dataset consisted of light intensity, humidity, air temperature, and total dissolved solids (TDS). Plant growth was measured by leaf area of the plant, number of leaves, and plant stem length every 3 to 4 days. The models used in the machine learning process were linear regression, polynomial regression, and random forest regression. The machine learning results showed that the best model for predicting plant growth was random forest regression with an MAE of 8.3% and an R2 of 0.93, for both bok coy and water spinach. The variables that influence growth the most are TDS and light intensity. According to the relationship between TDS gradient and plant growth gradient, the most optimal growth can be achieved by raising the TDS gradient or by maintaining a high TDS, which can be achieved by adding nutrient solution to the tank regularly.
{"title":"Study of the Effect of Physical Parameters on Commercial Hydroponics Based on Internet of Things (IoT): A Case Study of Bok Coy Plants (Brassica rapa) and Water Spinach (Ipomoea Aquatica)","authors":"M. Budiman, Efraim Partogi, A. Kristi, Prianka Anggara, N. Aminah","doi":"10.5614/j.math.fund.sci.2022.54.2.5","DOIUrl":"https://doi.org/10.5614/j.math.fund.sci.2022.54.2.5","url":null,"abstract":"Population growth causes the demand for food to increase. One solution that can be applied is agriculture with hydroponic technology. To increase production efficiency, one must know the physical parameters that most influence the production process. This research used an IoT system to gather accurate and precise measurement data of physical parameters to be used as a dataset for machine learning. The dataset consisted of light intensity, humidity, air temperature, and total dissolved solids (TDS). Plant growth was measured by leaf area of the plant, number of leaves, and plant stem length every 3 to 4 days. The models used in the machine learning process were linear regression, polynomial regression, and random forest regression. The machine learning results showed that the best model for predicting plant growth was random forest regression with an MAE of 8.3% and an R2 of 0.93, for both bok coy and water spinach. The variables that influence growth the most are TDS and light intensity. According to the relationship between TDS gradient and plant growth gradient, the most optimal growth can be achieved by raising the TDS gradient or by maintaining a high TDS, which can be achieved by adding nutrient solution to the tank regularly.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"60 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78856238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-19DOI: 10.5614/j.math.fund.sci.2022.54.2.4
Wayan Dasna, Ubed Sonai, Fahruddin Arrozi, H. W. Wijaya, Sutandyo Dwija Laksmana, S. Golhen
Two complexes of [Mn(Quin)2(N(CN)2)2]∞ (1) and [Co(Quin)2(N(CN)2)2]∞ (2) were synthesized and characterized. The as-synthesized complexes crystallized in a triclinic lattice with a space group of P-1. The unit cell parameter of 1 was a = 7.5207(14) Å, b = 7.7729(16) Å, c = 9.5968(15) Å, α = 96.388(6) o, β = 112.617(5) o, γ = 102.751(6) o, while for complex 2 it was a = 7.3942(10) Å, b = 7.7960(10) Å, c = 9.4907(13) Å, α = 96.631(4) o, β = 112.742(4) o, γ = 102.458(4) o. The magnetic properties and antimicrobial properties of both polymeric complexes were examined by magnetic susceptibility and diffusion techniques, respectively. Complexes 1 and 2 both showed antiferromagnetic properties and had a higher inhibitory effect on the growth of Staphylococcus aureus than Salmonella typhi.
{"title":"Synthesis, Structure, Magnetic and Antibacterial Properties of polymeric [M(Quin)2(N(CN)2)2]∞ (M = Mn(II), Co(II); Quin = quinoline)","authors":"Wayan Dasna, Ubed Sonai, Fahruddin Arrozi, H. W. Wijaya, Sutandyo Dwija Laksmana, S. Golhen","doi":"10.5614/j.math.fund.sci.2022.54.2.4","DOIUrl":"https://doi.org/10.5614/j.math.fund.sci.2022.54.2.4","url":null,"abstract":"Two complexes of [Mn(Quin)2(N(CN)2)2]∞ (1) and [Co(Quin)2(N(CN)2)2]∞ (2) were synthesized and characterized. The as-synthesized complexes crystallized in a triclinic lattice with a space group of P-1. The unit cell parameter of 1 was a = 7.5207(14) Å, b = 7.7729(16) Å, c = 9.5968(15) Å, α = 96.388(6) o, β = 112.617(5) o, γ = 102.751(6) o, while for complex 2 it was a = 7.3942(10) Å, b = 7.7960(10) Å, c = 9.4907(13) Å, α = 96.631(4) o, β = 112.742(4) o, γ = 102.458(4) o. The magnetic properties and antimicrobial properties of both polymeric complexes were examined by magnetic susceptibility and diffusion techniques, respectively. Complexes 1 and 2 both showed antiferromagnetic properties and had a higher inhibitory effect on the growth of Staphylococcus aureus than Salmonella typhi.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"29 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78798968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-04DOI: 10.5614/j.math.fund.sci.2022.54.2.3
E. Hidayati, Yulifa Handayani, I. Sudarma
Gyrinops versteegii is widely used in the perfume, incense, and cosmetic industries as a raw material. Other potential uses of the plant are being explored, including in food and medicine. Therefore, this study carried out antibacterial assay and gas chromatography-mass spectroscopy of G. versteegii fruit extracts in three solvents with different polarities, i.e., n-hexane, dichloromethane, and methanol. The agar well diffusion method was then used to test the extracts against Staphylococcus aureus and Escherichia coli. The dichloromethane extract showed the most effective antibacterial activity against S. aureus at a concentration of 40%, with a zone of inhibition of 13.17 mm compared to E. coli, for which it was 7 mm. Furthermore, the bacterial species showed different responses to the extracts. Total and partial inhibition was shown by the G. versteegii fruit extracts and E. coli, respectively, against S. aureus. Furthermore, GC-MS identified the following compounds in G. versteegii fruit extract: palmitic, oleic, and stearic acid, as well as bis-(2-ethylhexyl) phthalate, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, methyl octadec-9-enoate, squalene, and 2-monopalmitin derivates. The results showed antibacterial potency of G. versteegii fruit extract against pathogenic bacteria. Additionally, the antibacterial activity of the extract was stronger against S. aureus compared to E. coli.
龙涎香作为原料广泛应用于香水、熏香、化妆品等行业。人们正在探索这种植物的其他潜在用途，包括食品和医药。因此，本研究在正己烷、二氯甲烷和甲醇三种不同极性溶剂中对黄芪果实提取物进行了抑菌实验和气相色谱-质谱联用。采用琼脂孔扩散法检测提取物对金黄色葡萄球菌和大肠杆菌的抑制作用。二氯甲烷提取物对金黄色葡萄球菌的抑菌效果最好，浓度为40%，抑菌带为13.17 mm，对大肠杆菌的抑菌带为7 mm。不同菌种对不同提取物的反应也不同。葡萄果实提取物对金黄色葡萄球菌有完全抑制作用，大肠杆菌对金黄色葡萄球菌有部分抑制作用。此外，气相色谱-质谱联用技术还鉴定出了下列化合物:棕榈酸、油酸和硬脂酸，以及邻苯二甲酸二(2-乙基己基)酯、2,3-二氢-3,5-二羟基-6-甲基-4- h -吡喃-4-酮、十八烷基-9-烯酸甲酯、角鲨烯和2-单棕榈苷衍生物。结果表明，枳实提取物对病原菌具有较强的抑菌作用。此外，与大肠杆菌相比，提取物对金黄色葡萄球菌的抗菌活性更强。
{"title":"Antibacterial Activity of Gyrinops versteegii Fruit Extracts against Staphylococcus aureus and Escherichia coli and GC-MS Analysis","authors":"E. Hidayati, Yulifa Handayani, I. Sudarma","doi":"10.5614/j.math.fund.sci.2022.54.2.3","DOIUrl":"https://doi.org/10.5614/j.math.fund.sci.2022.54.2.3","url":null,"abstract":"Gyrinops versteegii is widely used in the perfume, incense, and cosmetic industries as a raw material. Other potential uses of the plant are being explored, including in food and medicine. Therefore, this study carried out antibacterial assay and gas chromatography-mass spectroscopy of G. versteegii fruit extracts in three solvents with different polarities, i.e., n-hexane, dichloromethane, and methanol. The agar well diffusion method was then used to test the extracts against Staphylococcus aureus and Escherichia coli. The dichloromethane extract showed the most effective antibacterial activity against S. aureus at a concentration of 40%, with a zone of inhibition of 13.17 mm compared to E. coli, for which it was 7 mm. Furthermore, the bacterial species showed different responses to the extracts. Total and partial inhibition was shown by the G. versteegii fruit extracts and E. coli, respectively, against S. aureus. Furthermore, GC-MS identified the following compounds in G. versteegii fruit extract: palmitic, oleic, and stearic acid, as well as bis-(2-ethylhexyl) phthalate, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, methyl octadec-9-enoate, squalene, and 2-monopalmitin derivates. The results showed antibacterial potency of G. versteegii fruit extract against pathogenic bacteria. Additionally, the antibacterial activity of the extract was stronger against S. aureus compared to E. coli.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88864739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-28DOI: 10.5614/j.math.fund.sci.2022.54.2.1
A. S. Eegunjobi, O. Makinde
The biggest public health problem facing the whole world today is the COVID-19 pandemic. From the time COVID-19 came into the limelight, people have been losing their loved ones and relatives as a direct result of this disease. Here, we present a six-compartment epidemiological model that is deterministic in nature for the emergence and spread of two strains of the COVID-19 disease in a given community, with quarantine and recovery due to treatment. Employing the stability theory of differential equations, the model was qualitatively analyzed. We derived the basic reproduction number for both strains and investigated the sensitivity index of the parameters. In addition to this, we probed the global stability of the disease-free equilibrium. The disease-free equilibrium was revealed to be globally stable, provided and the model exhibited forward bifurcation. A numerical simulation was performed, and pertinent results are displayed graphically and discussed.
{"title":"Mathematical Analysis of Two Strains Covid-19 Disease Using SEIR Model","authors":"A. S. Eegunjobi, O. Makinde","doi":"10.5614/j.math.fund.sci.2022.54.2.1","DOIUrl":"https://doi.org/10.5614/j.math.fund.sci.2022.54.2.1","url":null,"abstract":"The biggest public health problem facing the whole world today is the COVID-19 pandemic. From the time COVID-19 came into the limelight, people have been losing their loved ones and relatives as a direct result of this disease. Here, we present a six-compartment epidemiological model that is deterministic in nature for the emergence and spread of two strains of the COVID-19 disease in a given community, with quarantine and recovery due to treatment. Employing the stability theory of differential equations, the model was qualitatively analyzed. We derived the basic reproduction number for both strains and investigated the sensitivity index of the parameters. In addition to this, we probed the global stability of the disease-free equilibrium. The disease-free equilibrium was revealed to be globally stable, provided and the model exhibited forward bifurcation. A numerical simulation was performed, and pertinent results are displayed graphically and discussed.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"35 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76153895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-18DOI: 10.5614/j.math.fund.sci.2022.54.1.10
A. Dinoto, Z. Noviana, O. Efendy, H. Julistiono
Blister beetles are an important object of study in the field of agriculture and health. Despite being known to be important for the host animal, the gut bacteria of blister beetles have not been investigated deeply. This study aimed to provide the first initial description of the gut bacterial community of Mylabris pustulata as one species of blister beetles, based on a culture-independent technique. Adult blister beetles of the same colony were sampled and confirmed as Mylabris pustulata Thunberg. The gut content was used in high throughput sequencing, targeting the V3-V4 regions of 16S rRNA gene, and in the cultivation of resident bacteria. The results showed that higher bacterial richness was present in the gut communities when compared to the grown bacterial culture. Proteobacteria was confirmed as the most abundant phylum in the gut of M. pustulata, whereby most reads belonged to the class of Gammaproteobacteria. The dominant bacterial genera were determined as Enterobacter, Acinetobacter, Enterococcus, Klebsiella, and Pseudomonas. In addition, our cultivation attempts led to successful isolation of members of Klebsiella and Enterococcus. The subject of this study could be explored further to find the potential roles of these microbiota in the gut of the specific beetles and their bioprospects.
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