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Genome-wide investigation of defensin genes in apple (Malus×domestica Borkh.) and in vivo analyses reveal that MdDEF25 confers resistance to Fusarium solani 对苹果(Malus×domestica Borkh.)中防御素基因的全基因组调查和体内分析表明,MdDEF25 可赋予苹果对 Fusarium solani 的抗性
IF 4.8 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-11 DOI: 10.1016/j.jia.2024.03.039
Mengli Yang, Jian Jiao, Yiqi Liu, Ming Li, Yan Xia, Feifan Hou, Chuanmi Huang, Hengtao Zhang, Miaomiao Wang, Jiangli Shi, Ran Wan, Kunxi Zhang, Pengbo Hao, Tuanhui Bai, Chunhui Song, Jiancan Feng, Xianbo Zheng
Apple replant disease is a complex soil syndrome that occurs when the same fields are used repeatedly as apple orchards. Causes include different pathogens, with () as the main pathogen. disrupts the structure and function of the orchard soil ecosystem and inhibits the growth and development of apple trees, significantly impacting the quality and yield of apples. In this study, we compared the transcriptomes of uninoculated apple saplings with those inoculated with . The differentially expressed genes were mainly enriched in processes such as response to symbiotic fungus and included several defensins. Plant defensins are antimicrobial peptides, but their roles during infection by remain unclear. We performed a genome-wide identification of apple defensin genes and identified 25 genes with the conserved motif of eight cysteine residues. In wild-type apple rootstock inoculated with , the root surface cells experienced severe damage, and significant differences were observed in the total root length, total root projection area, root tips, root forks, and the total root surface area compared to the control group. qRT-PCR analysis revealed that and were triggered in response to infection in apples. Subcellular localization showed specific expression of MdDEF3-YFP and MdDEF25-YFP proteins at the cell membrane. Overexpressing the fusion increased resistance against in apple, providing a new strategy for the future prevention and biological control of apple replant disease.
苹果移栽病是一种复杂的土壤综合症,当同一块田地被反复用作苹果园时就会发生。原因包括不同的病原体,其中()是主要病原体。它破坏果园土壤生态系统的结构和功能,抑制苹果树的生长和发育,严重影响苹果的质量和产量。在这项研究中,我们比较了未接种()和接种()的苹果树苗的转录组。差异表达的基因主要集中在对共生真菌的反应等过程中,其中包括几种防御素。植物防御素是一种抗菌肽,但它们在感染过程中的作用仍不清楚。我们对苹果防御素基因进行了全基因组鉴定,发现25个基因具有8个半胱氨酸残基的保守基团。在野生型苹果根茎中接种了Ⅳ号基因后,根表面细胞受到严重破坏,与对照组相比,根的总长度、根的总投影面积、根尖、根叉和根的总表面积都有显著差异。亚细胞定位显示,MdDEF3-YFP 和 MdDEF25-YFP 蛋白在细胞膜上特异表达。过量表达融合蛋白可提高苹果的抗病性,为今后预防和生物防治苹果移栽病提供了新的策略。
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引用次数: 0
Comparison of CWSI and Ts-Ta-VIs in moisture monitoring of dryland crops (sorghum, maize) based on UAV remote sensing 基于无人机遥感的 CWSI 和 Ts-Ta-VI 在旱地作物(高粱、玉米)水分监测中的比较
IF 4.8 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-11 DOI: 10.1016/j.jia.2024.03.042
Hui Chen, Hongxing Chen, Song Zhang, Shengxi Chen, Fulang Cen, Quanzhi Zhao, Xiaoyun Huang, Tengbing He, Zhenran Gao
Monitoring agricultural drought using remote sensing data is crucial for precision irrigation in modern agriculture. Utilizing unmanned aerial vehicle (UAV) remote sensing, this study explored the applicability of an empirical crop water stress index (CWSI) based on canopy temperature and three-dimensional drought indices (TDDI) constructed from surface temperature (), air temperature () and five vegetation indices (VIs) for monitoring the moisture status of dryland crops. Three machine learning algorithms (random forest regression [RFR], support vector regression, and partial least squares regression) were used to compare the performance of the drought indices for vegetation moisture content (VMC) estimation in sorghum and maize. The main results of the study were as follows: (1) Comparative analysis of the drought indices revealed that --Normalized Difference Vegetation Index (TDDIn) and --Enhanced Vegetation Index (TDDIe) were more strongly correlated with VMC compared with the other indices. The indices exhibited varying sensitivities to VMC under different irrigation regimes; the strongest correlation observed was for the TDDIe index with maize under the fully irrigated treatment (=−0.93); (2) Regarding spatial and temporal characteristics, the TDDIn, TDDIe and CWSI indices showed minimal differences over the experimental period, with coefficients of variation were 0.25, 0.18 and 0.24, respectively. All three indices were capable of effectively characterizing the moisture distribution in dryland maize and sorghum crops, but the TDDI indices more accurately monitored the spatial distribution of crop moisture after a rainfall or irrigation event. (3) For prediction of the moisture content of single crops, RFR models based on TDDIn and TDDIe estimated VMC most accurately (0.7), and the TDDIn-based model predicted VMC with the highest accuracy when considering multiple-crop samples, with and RMSE of 0.62 and 14.26%, respectively. Thus, TDDI proved more effective than the CWSI in estimating crop water content.
利用遥感数据监测农业干旱对于现代农业的精准灌溉至关重要。本研究利用无人飞行器(UAV)遥感技术,探索了基于冠层温度的经验作物水分胁迫指数(CWSI)和由地表温度()、空气温度()和五个植被指数(VIs)构建的三维干旱指数(TDDI)在监测旱地作物水分状况方面的适用性。研究采用了三种机器学习算法(随机森林回归、支持向量回归和偏最小二乘回归)来比较干旱指数在估算高粱和玉米植被含水量(VMC)方面的性能。研究的主要结果如下:(1) 干旱指数的比较分析表明,归一化差异植被指数(TDDIn)和增强植被指数(TDDDIe)与植被含水量的相关性比其他指数更强。(2) 在时空特征方面,TDDIn、TDDDIe 和 CWSI 指数在试验期间的差异极小,变异系数分别为 0.25、0.18 和 0.24。这三个指数都能有效描述旱地玉米和高粱作物的水分分布特征,但 TDDI 指数能更准确地监测降雨或灌溉事件后作物水分的空间分布。(3) 在预测单一作物的水分含量时,基于 TDDIn 和 TDDDIe 的 RFR 模型对 VMC 的估算最为准确(0.7),而在考虑多作物样本时,基于 TDDIn 的模型预测 VMC 的准确度最高,均方根误差分别为 0.62% 和 14.26%。因此,在估算作物含水量方面,TDDDI 比 CWSI 更有效。
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引用次数: 0
Quantifying source-sink relationships in leaf-colour modified rice genotypes during grain filling 谷物灌浆期叶色改良水稻基因型的源汇关系量化
IF 4.8 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-07 DOI: 10.1016/j.jia.2024.03.034
Zhenxiang Zhou, Paul C. Struik, Junfei Gu, Peter E.L. van der Putten, Zhiqin Wang, Jianchang Yang, Xinyou Yin
Leaf-colour modification can affect the canopy photosynthesis, with a potential effect on rice yield and yield components. Also, modulating source-sink relationships through crop management has been used to improve crop productivity. This study aims to investigate whether and how modifying leaf colour alters source-sink relationships and whether current crop cultivation practices remain applicable for leaf-colour modified genotypes. Periodically collected data of total biomass and nitrogen (N) accumulation in rice genotypes of four genetic backgrounds and their leaf-colour modified (greener or yellower) variants were analysed, using a recently established model method to quantify the source-sink (im)balance during grain filling. Among all leaf-colour variants, only one yellower-leaf variant showed higher source capacity than its normal genotype. This was associated with increased post-flowering N-uptake that prolonged functional leaf-N duration, and this increased post-flowering N-uptake was possible because of reduced pre-flowering N-uptake. The density experiment showed that current management practices (insufficient planting density accompanied with abundant N application) were unsuitable for the yellower-leaf genotype, ultimately limiting its yield potential. Leaf-colour modification affects source-sink relationships by regulating N trade-off between pre-flowering and post-flowering uptake, and N translocation between source and sink organs. To best exploit leaf-colour modification for an improved crop productivity, adjustments of crop management practices are required.
叶色变化会影响冠层光合作用,从而对水稻产量和产量成分产生潜在影响。此外,通过作物管理调节源汇关系也被用来提高作物产量。本研究旨在探讨改变叶色是否以及如何改变源汇关系,以及目前的作物栽培方法是否仍然适用于叶色改变的基因型。利用最近建立的模型方法,对四种遗传背景的水稻基因型及其叶色修饰(更绿或更黄)变体的总生物量和氮(N)积累进行了定期收集的数据分析,以量化谷物灌浆期间的源-汇(不)平衡。在所有叶色变体中,只有一个叶色较黄的变体比其正常基因型表现出更高的源能力。这与花后氮吸收增加有关,延长了功能性叶-氮持续时间,花后氮吸收增加是因为花前氮吸收减少。密度实验表明,目前的管理方法(种植密度不足,同时施用大量氮)不适合黄叶基因型,最终限制了其产量潜力。叶色修饰通过调节花前和花后吸收氮的权衡,以及源器官和吸收器官之间的氮转移,影响源-汇关系。要充分利用叶色变化提高作物产量,就必须调整作物管理方法。
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引用次数: 0
Mining elite loci and candidate genes for root morphology-related traits at seedling stage by genome-wide association studies in Upland Cotton (Gossypium hirsutum L.) 通过对陆地棉(Gossypium hirsutum L.)的全基因组关联研究挖掘苗期根系形态相关性状的精英位点和候选基因
IF 4.8 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-07 DOI: 10.1016/j.jia.2024.03.037
Huaxiang Wu, Xiaohui Song, Muhammad Waqas-Amjid, Chuan Chen, Dayong Zhang, Wangzhen Guo
The root system architecture plays an essential role in water and nutrient acquisition in plants and is significantly involved in plant adaptation to various environmental stresses. In this study, a panel of 242 cotton accessions was collected to investigate six root morphological traits at the seedling stage, including main root length (MRL), root fresh weight (RFW), total root length (TRL), root surface area (RSA), root volume (RV), and root average diameter (AvgD). The correlation analysis between six root morphological traits revealed a strong positive correlation of TRL with RSA, RV with RSA and AvgD, whereas a significant negative correlation between TRL and AvgD. Subsequently, a genome-wide association study (GWAS) was performed using the root phenotypic data and genotypic data reported previously for 242 accessions using 56,010 single nucleotide polymorphisms (SNPs) from the CottonSNP80K array. A total of 41 quantitative trait loci (QTLs) were identified with 9 for MRL, 6 for RFW, 9 for TRL, 12 for RSA, 12 for RV and 2 for AvgD respectively. Among them, 8 QTLs were repeatedly detected in two or more traits. Integrated with transcriptome analysis, we identified 17 candidate genes with high transcripts of transcripts per million (TPM)≥30 in roots. Furthermore, we verified functionally a candidate gene encoding a WPP domain protein 2 in root development. Virus-induced gene silencing (VIGS) assay showed that knocking down significantly inhibited root development in cotton, indicating its positive role in root system architecture formation. Together, these results provide a theoretical basis and candidate genes for cotton root developmental biology and root-related cotton breeding.
根系结构在植物获取水分和养分的过程中起着至关重要的作用,并在植物适应各种环境胁迫的过程中发挥着重要作用。本研究收集了 242 个棉花品种,研究了幼苗期的六个根系形态特征,包括主根长度(MRL)、根鲜重(RFW)、总根长度(TRL)、根表面积(RSA)、根体积(RV)和根平均直径(AvgD)。六种根形态性状之间的相关分析表明,TRL与RSA、RV与RSA和AvgD呈强正相关,而TRL与AvgD呈显著负相关。随后,利用之前报告的 242 个品种的根表型数据和基因型数据,使用 CottonSNP80K 阵列中的 56,010 个单核苷酸多态性(SNPs)进行了全基因组关联研究(GWAS)。共鉴定出 41 个数量性状位点(QTLs),分别为 9 个 MRL、6 个 RFW、9 个 TRL、12 个 RSA、12 个 RV 和 2 个 AvgD。其中,8 个 QTL 在两个或两个以上性状中重复检测到。结合转录组分析,我们在根中发现了 17 个转录本≥30 的高转录本候选基因。此外,我们还从功能上验证了一个在根系发育过程中编码 WPP 结构域蛋白 2 的候选基因。病毒诱导的基因沉默(VIGS)试验表明,敲除该基因会显著抑制棉花根系的发育,表明其在根系结构形成中的积极作用。这些结果为棉花根系发育生物学和与根系相关的棉花育种提供了理论依据和候选基因。
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引用次数: 0
Brassinosteroids improve the redox state of wheat florets under low-nitrogen stress and alleviate degeneration 芸苔素类固醇改善小麦小花在低氮胁迫下的氧化还原状态并缓解退化现象
IF 4.8 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-07 DOI: 10.1016/j.jia.2024.03.035
Zimeng Liang, Juan Li, Jingyi Feng, Zhiyuan Li, Vinay Nangia, Fei Mo, Yang Liu
Reducing nitrogen application rates can mitigate issues such as environmental degradation and resource wastage. However, it can also exacerbate problems such as wheat floret degeneration, leading to reduced yields. Therefore, investigating wheat floret degeneration mechanisms under low nitrogen stress and identifying mitigation measures are conducive to achieving high yields and sustainable development. To investigate the physiological mechanism of low nitrogen stress affecting wheat floret degradation and whether exogenous brassinosteroids can alleviate this stress, three nitrogen application rates (N0, no nitrogen application; N1, 120 kg ha pure nitrogen; and N2, 240 kg ha pure nitrogen) and exogenous spraying experiments (N0CK, no nitrogen with water spraying; N0BR, no nitrogen with 24-epibrassinolide (an active brassinosteroids) spraying; and N1, 120 kg ha pure nitrogen with water spraying) were designed. The results indicated that low nitrogen stress induced a large amount of reactive oxygen species generation. Although wheat spikes synthesized flavonoids to combat oxidative stress, their energy metabolism (glycolysis and tricarboxylic acid cycle) and ascorbate-glutathione cycle were inhibited, keeping reactive oxygen levels elevated within the spike, inducing cell death and exacerbating floret degeneration. Furthermore, brassinosteroids played a role in regulating wheat floret degeneration under low-nitrogen stress. Exogenous foliar spraying of 24-epibrassinolide promoted energy metabolism and the ascorbate-glutathione cycle within the spike, enhancing energy charge and effectively mitigating a portion of reactive oxygen induced by low nitrogen stress, thereby alleviating floret degeneration caused by low nitrogen stress. In summary, low-nitrogen stress disrupts the redox homeostasis of wheat spikes, leading to floret degeneration. Brassinosteroids alleviate floret degeneration by improving the redox state of wheat spikes. This research provides theoretical support for balancing the contradiction between high yields and sustainable development and is beneficial for the application of low nitrogen in production.
降低氮肥施用量可以缓解环境退化和资源浪费等问题。但同时也会加剧小麦小花退化等问题,导致减产。因此,研究小麦在低氮胁迫下小花退化的机理并找出缓解措施,有利于实现高产和可持续发展。为了研究低氮胁迫影响小麦小花退化的生理机制以及外源铜皮素类固醇是否能缓解这种胁迫,研究人员采用了三种施氮量(N0,不施氮;N1,120 kg ha 纯氮;设计了三种施氮量(N0,不施氮;N1,120 千克/公顷纯氮;N2,240 千克/公顷纯氮)和外源喷洒试验(N0CK,不施氮,喷水;N0BR,不施氮,喷洒 24-环黄铜内酯(一种活性黄铜类固醇);N1,120 千克/公顷纯氮,喷水)。结果表明,低氮胁迫诱导产生大量活性氧。虽然小麦穗能合成类黄酮来对抗氧化胁迫,但其能量代谢(糖酵解和三羧酸循环)和抗坏血酸-谷胱甘肽循环受到抑制,使穗内活性氧水平持续升高,诱导细胞死亡,加剧小花退化。此外,黄铜类固醇在低氮胁迫下对小花退化起调节作用。外源叶面喷施 24-epibrassinolide 可促进穗内能量代谢和抗坏血酸-谷胱甘肽循环,增强能量充电,有效缓解低氮胁迫诱导的部分活性氧,从而减轻低氮胁迫引起的小花退化。总之,低氮胁迫破坏了小麦穗的氧化还原平衡,导致小花退化。芸苔素类固醇通过改善小麦穗的氧化还原状态来缓解小花退化。这项研究为平衡高产与可持续发展之间的矛盾提供了理论支持,有利于低氮在生产中的应用。
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引用次数: 0
Pectin methylesterase inhibitors GhPMEI53 and AtPMEI19 improve seed germination by modulating cell wall plasticity in cotton and Arabidopsis 果胶甲基酯酶抑制剂 GhPMEI53 和 AtPMEI19 通过调节棉花和拟南芥细胞壁的可塑性提高种子萌发率
IF 4.8 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-07 DOI: 10.1016/j.jia.2024.03.036
Yayue Pei, Yakong Wang, Zhenzhen Wei, Ji Liu, Yonghui Li, Shuya Ma, Ye Wang, Fuguang Li, Jun Peng, Zhi Wang
The germination process of seeds is influenced by the interplay between two opposing factors: pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI), which collectively regulate patterns of pectin methylesterification. Despite the recognized importance of pectin methylesterification in seed germination, the specific mechanisms that govern this process remain unclear. In this study, we demonstrated that the overexpression of is associated with a decrease in PME activity and an increase in pectin methylesterification. This leads to the softening of the cell wall in seeds, which positively regulates cotton seed germination. AtPMEI19, the homologue in , plays a similar role in seed germination to GhPMEI53, indicating a conserved function and mechanism of PMEI in seed germination regulation. Further studies revealed that GhPMEI53 and AtPMEI19 directly contribute to promoting radicle protrusion and seed germination by inducing cell wall softening and reducing mechanical strength. Additionally, the pathways of ABA and GA in the transgenic materials underwent significant changes, suggesting that GhPMEI53/AtPMEI19-mediated pectin methylesterification serves as a regulatory signal for the related phytohormones involved in seed germination. In summary, GhPMEI53 and its homologs alter the mechanical properties of cell walls, influencing the mechanical resistance of the endosperm or testa. Moreover, they impact cellular phytohormone pathways (e.g., ABA, GA) to regulate seed germination. These findings enhance our understanding of pectin methylesterification in cellular morphological dynamics and signaling transduction, and contribute to a more comprehensive understanding of the PME/PMEI super-gene family in plants.
种子的萌发过程受到果胶甲基酯酶(PME)和果胶甲基酯酶抑制剂(PMEI)这两个对立因子之间相互作用的影响,它们共同调节果胶甲基酯化的模式。尽管果胶甲基化在种子萌发过程中的重要性已得到公认,但调控这一过程的具体机制仍不清楚。在这项研究中,我们证明了 PME 的过表达与 PME 活性的降低和果胶甲基化的增加有关。这导致了种子细胞壁的软化,从而对棉花种子的萌发起到了积极的调节作用。与 GhPMEI53 同源的 AtPMEI19 与 GhPMEI53 在种子萌发中的作用相似,表明 PMEI 在种子萌发调控中的功能和机制是保守的。进一步研究发现,GhPMEI53 和 AtPMEI19 通过诱导细胞壁软化和降低机械强度,直接促进胚根突起和种子萌发。此外,转基因材料中 ABA 和 GA 的作用途径也发生了显著变化,表明 GhPMEI53/AtPMEI19 介导的果胶甲基酯化作用是参与种子萌发的相关植物激素的调控信号。总之,GhPMEI53 及其同源物会改变细胞壁的机械特性,影响胚乳或种皮的机械阻力。此外,它们还影响细胞植物激素途径(如 ABA、GA),从而调节种子萌发。这些发现加深了我们对果胶甲基酯化在细胞形态动力学和信号转导中的作用的理解,有助于我们更全面地了解植物中的 PME/PMEI 超级基因家族。
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引用次数: 0
Crossing latitude introduction delayed flowering and facilitated dry matter accumulation of soybean as a forage crop 跨纬度引入延迟了大豆作为饲料作物的开花期,并促进了其干物质的积累
IF 4.8 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-07 DOI: 10.1016/j.jia.2024.03.033
Dong An, Xingfa Lai, Tianfu Han, Jean Marie Vianney Nsigayehe, Guixin Li, Yuying Shen
Grain soybean [ (L.) Merr] shows potential as a forage crop following introduction from low to high due to the growth phenotype alteration. However, the application of this approach is impeded by the diverse ecological types of soybeans, their adaptability to the introduction, and the uncertainty surrounding the growth phenotype post-introduction. In this study, 24 grain soybean varieties from low-latitude regions (22-31°N) were introduced to the high-latitude northwestern Loess Plateau (39°N) between 2018 and 2019. The growth phenotypes were observed, and their forage productivity potential was assessed. All varieties displayed delayed flowering following cross-latitude introduction, with the vegetative growth phase of some varieties even exceeding their entire growth duration at their origin. Rapid dry matter accumulation rates were indicative of growth adaptation performance and formed the basis for soybean forage yield. Varieties from the tropical south China region exhibited significant yield advantages, with dry matter yields of 8.97-14.68 t ha and crude protein yields of 1.44-2.51 t ha. Varieties HX3 and GX7, originating from this region, demonstrated optimal growth adaptability and productivity in the cross-latitude environment, achieving the highest dry matter yields of 14.68 and 13.86 t ha, respectively. As a result, HX3 and GX7 are recommended for local farming systems to provide high-quality forage. The cross-latitude introduction of soybean is proposed as a viable and efficient strategy for forage improvement and application.
由于生长表型的改变,谷物大豆[(L. )Merr]在从低到高的引入后显示出作为饲料作物的潜力。然而,大豆生态类型的多样性、对引种的适应性以及引种后生长表型的不确定性阻碍了这一方法的应用。在本研究中,2018 年至 2019 年期间,24 个来自低纬度地区(22-31°N)的谷物大豆品种被引入高纬度西北黄土高原(39°N)。对其生长表型进行了观察,并评估了其饲料生产潜力。所有品种在跨纬度引种后都表现出延迟开花,一些品种的无性生长期甚至超过了它们在原产地的整个生长期。快速的干物质积累率是生长适应性表现的标志,也是大豆饲草产量的基础。华南热带地区的品种表现出显著的产量优势,干物质产量为 8.97-14.68 吨/公顷,粗蛋白产量为 1.44-2.51 吨/公顷。来自该地区的品种 HX3 和 GX7 在跨纬度环境中表现出最佳的生长适应性和生产力,干物质产量最高,分别达到 14.68 吨/公顷和 13.86 吨/公顷。因此,HX3 和 GX7 被推荐用于当地农业系统,以提供优质饲草。跨纬度引种大豆是一种可行且高效的饲草改良和应用策略。
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引用次数: 0
Optimizing tillage and fertilization practices to improve the carbon footprint and energy efficiency of wheat-maize cropping systems 优化耕作和施肥方法,改善小麦-玉米种植系统的碳足迹和能源效率
IF 4.8 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-06 DOI: 10.1016/j.jia.2024.03.026
Kun Han, Xinzhu Li, Liang Jia, Dazhao Yu, Wenhua Xu, Hongkun Chen, Tao Song, Peng Liu
To make agricultural systems sustainable in terms of their greenness and efficiency, it is essential to optimize the tillage and fertilization practices. To assess the effect of tilling and fertilization practices in the wheat-maize cropping systems, we carried out a three-year field experiment designed to quantify the carbon footprint (CF), and energy efficiency of the cropping systems in the North China Plain. As the study parameters, we used four tillage practices (no tillage (NT), conventional tillage (CT), rotary tillage (RT) and subsoiling rotary tillage (SRT)), and two fertilizer regimes (inorganic fertilizer (IF), and hybrid fertilizer with organic and inorganic components (HF)). Our results indicated that the most prominent energy inputs and greenhouse gas (GHG) emissions could be ascribed to the use of fertilizers and fuel consumption. Assuming the same fertilization regime, ranking the tillage patterns with respect to the value of the crop yield, the profit, the CF, the energy use efficiency (EUE) or the energy productivity (EP) for either wheat or maize always gave the following result: SRT>RT>CT>NT. For the same tillage, the energy consumption associated with HF was higher than IF, but its GHG emissions and CF were lower while the yield and profit were better. In terms of the overall performance, tilling is more beneficial than NT, and reduced tillage (RT and SRT) are more beneficial than CT. The fertilization regime with the best overall performance was HF. Combining SRT with HF has significant potential for reducing CF and increasing EUE, improving the sustainability. Adopting measures promoting these optimizations can help overcome the challenges posed by lack of food security, energy crises and ecological stress.
为了使农业系统在绿色和效率方面具有可持续性,必须优化耕作和施肥方法。为了评估耕作和施肥方法对小麦-玉米种植系统的影响,我们在华北平原开展了一项为期三年的田间试验,旨在量化种植系统的碳足迹(CF)和能源效率。作为研究参数,我们采用了四种耕作方式(免耕(NT)、常规耕作(CT)、旋耕(RT)和覆土旋耕(SRT))和两种施肥制度(无机肥(IF)和有机无机混合肥(HF))。我们的研究结果表明,最主要的能源投入和温室气体(GHG)排放可归因于肥料的使用和燃料消耗。在相同施肥制度下,根据小麦或玉米的作物产量值、利润、CF、能源利用效率(EUE)或能源生产率(EP)对耕作模式进行排序,结果如下:SRT>RT>CT>NT。在相同耕作条件下,HF 的能耗高于 IF,但温室气体排放量和 CF 更低,产量和利润更高。从整体表现来看,翻耕比 NT 更有利,减耕(RT 和 SRT)比 CT 更有利。综合表现最好的施肥制度是 HF。将 SRT 与 HF 结合使用,在减少 CF 和增加 EUE 方面具有巨大潜力,从而提高了可持续性。采取促进这些优化的措施有助于克服缺乏粮食安全、能源危机和生态压力带来的挑战。
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引用次数: 0
Pod-shattering characteristic differences between shattering-resistant and shattering-susceptible common vetch accessions are associated with lignin biosynthesis 抗碎裂和易碎裂普通薇甘菊品种的碎荚特征差异与木质素的生物合成有关
IF 4.8 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-06 DOI: 10.1016/j.jia.2024.03.032
Xueming Dong, Jiwei Chen, Qiang Zhou, Dong Luo, Longfa Fang, Wenxian Liu, Zhipeng Liu
The common vetch ( L.) is a self-pollinated annual forage legume that is widely distributed worldwide. It has wide adaptability and high nutritional value and is commonly used as an important protein source for livestock feed. However, pod shattering seriously limits the yield of common vetch. To clarify the mechanism of pod shattering in common vetch, the pod walls of three shattering-resistant (SR) accessions (B65, B135, and B392) and three shattering-susceptible (SS) accessions (L33, L170, and L461) were selected for transcriptome sequencing. A total of 17190 differentially expressed genes (DEGs) were identified in the pod wall of B135 and L461 common vetch at 5, 10, 15, 20, and 25 days after anthesis. KEGG analysis showed that “phenylpropanoid biosynthesis” was the most significantly enriched pathway, and 40 structural genes associated with lignin biosynthesis were identified and differentially expressed in B135 and L461 common vetch. We analysed the DEGs in the pod wall of three SR and three SS accessions at 15 days after anthesis, and most of the DEGs were consistent with the significant enrichment pathways identified in B135 and L461 common vetch. The total lignin content of SR accessions was significantly lower than the SS accessions. The present study lays a foundation for understanding the molecular regulatory mechanism of pod shattering related to lignin biosynthesis in common vetch and provides reference functional genes for breeders to further cultivate shattering-resistant common vetch varieties.
普通矢车菊(L. )是一种自花授粉的一年生饲料豆科植物,广泛分布于世界各地。它适应性广,营养价值高,通常用作牲畜饲料的重要蛋白质来源。然而,豆荚破碎严重限制了普通薇菜的产量。为了弄清普通矢车菊豆荚破碎的机理,研究人员选择了三个抗破碎(SR)品种(B65、B135 和 B392)和三个易破碎(SS)品种(L33、L170 和 L461)的豆荚壁进行转录组测序。在开花后 5、10、15、20 和 25 天,在 B135 和 L461 普通薇菜的荚果壁上共鉴定出 17190 个差异表达基因(DEGs)。KEGG 分析表明,"苯丙类生物合成 "是最显著富集的途径,在 B135 和 L461 常见薇菜中发现了 40 个与木质素生物合成相关的结构基因,并且这些基因在 B135 和 L461 常见薇菜中有差异表达。我们分析了花后 15 天 3 个 SR 和 3 个 SS 接种荚果壁中的 DEGs,大多数 DEGs 与在 B135 和 L461 普通薇菜中发现的显著富集途径一致。SR 接种的木质素总含量明显低于 SS 接种。本研究为了解与木质素生物合成相关的荚果破碎分子调控机制奠定了基础,并为育种者进一步培育抗破碎荚果的芸苔品种提供了参考功能基因。
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引用次数: 0
Can a delayed sowing date improve the eating and cooking quality of mechanically transplanted rice in the Sichuan basin? 推迟播种期能否改善四川盆地机插秧水稻的食用和烹饪品质?
IF 4.8 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-06 DOI: 10.1016/j.jia.2024.03.023
Yuxin He, Fei Deng, Chi Zhang, Qiuping Li, Xiaofan Huang, Chenyan He, Xiaofeng Ai, Yujie Yuan, Li Wang, Hong Cheng, Tao Wang, Youfeng Tao, Wei Zhou, Xiaolong Lei, Yong Chen, Wanjun Ren
Adjustment of the sowing date is a widely used measure in rice production to adapt to high-temperature conditions. However, the impact of delayed sowing date (DS) on rice quality may vary by variety and ecological conditions. In this study, we conducted experiments using different sowing dates, that is, conventional sowing date 1 (CS1), CS2 (10 d later than CS1), DS1 (30 d later than CS1), and DS2 (30 d later than CS2), and three rice varieties, i.e., “Yixiangyou 2115,” “Fyou 498,” and “Chuanyou 6203.” This experiment was conducted at four sites in the Sichuan basin in 2018 and 2019 to evaluate the influence of DS on the pasting properties of rice, which are a proxy for eating and cooking quality (ECQ). In DS1 and DS2, rice had a significantly greater amylose content (AC) but a lower protein content (PC), peak viscosity (PKV), cool paste viscosity (CPV), and hot paste viscosity (HPV) than in CS1 and CS2. Moreover, with the exception of CS2 and DS1 in 2018, DS1 and DS2 led to a 2.15–11.19% reduction in breakdown viscosity (BDV) and a 23.46–108.47% increase in setback viscosity (SBV). However, the influence of DS on rice pasting properties varied by study site and rice variety. In 2019, DS1 and DS2 led to a BDV reduction of 2.35–9.33, 2.61–8.61, 10.03–17.78, and 2.06–8.93%, and a SBV increase of 2.32–60.93, 63.74–144.24, 55.46–91.63, and -8.28–65.37% at the Dayi, Anzhou, Nanbu, and Shehong, respectively. DS resulted in a greater decrease in PKV, HPV, CPV, and BDV and a greater increase in the AC and SBV for Yixiangyou 2115 than for Chuanyou 6203 and Fyou 498. Correlation analysis indicated that PKV and HPV were significantly and positively related to the mean, maximum, and minimum temperatures after heading. These temperatures have to be greater than 25.9, 31.2, and 22.3℃ to increase the relative BDV and decrease the relative SBV of rice, thereby enhancing ECQ. In conclusion, DS might contribute to a significant deterioration in ECQ in machine-transplanted rice in the Sichuan basin. A mean temperature above 25.9℃ after heading is required to improve the ECQ of rice.
调整播种期是水稻生产中广泛采用的一项措施,以适应高温条件。然而,延迟播种期(DS)对稻米品质的影响可能因品种和生态条件而异。在本研究中,我们使用不同的播种日期,即常规播种日期 1(CS1)、CS2(比 CS1 晚 10 天)、DS1(比 CS1 晚 30 天)和 DS2(比 CS2 晚 30 天),以及三个水稻品种,即 "宜香优 2115"、"福优 498 "和 "川优 6203",进行了试验。该试验于 2018 年和 2019 年在四川盆地的 4 个地点进行,旨在评估 DS 对大米糊化性的影响,而糊化性是食用和烹饪品质(ECQ)的代表。与CS1和CS2相比,DS1和DS2的大米直链淀粉含量(AC)显著增加,但蛋白质含量(PC)、峰值粘度(PKV)、冷糊化粘度(CPV)和热糊化粘度(HPV)却较低。此外,除2018年的CS2和DS1外,DS1和DS2导致分解粘度(BDV)降低2.15-11.19%,挫粘度(SBV)增加23.46-108.47%。然而,DS 对水稻糊化性能的影响因研究地点和水稻品种而异。2019年,DS1和DS2在大邑、安州、南部和射洪分别导致BDV降低2.35-9.33%、2.61-8.61%、10.03-17.78%和2.06-8.93%,SBV增加2.32-60.93%、63.74-144.24%、55.46-91.63%和-8.28-65.37%。与川优 6203 和福优 498 相比,DS 导致宜香优 2115 的 PKV、HPV、CPV 和 BDV 下降幅度更大,AC 和 SBV 上升幅度更大。相关分析表明,PKV 和 HPV 与打顶后的平均温度、最高温度和最低温度显著正相关。这些温度必须高于 25.9、31.2 和 22.3℃,才能增加水稻的相对 BDV 和降低相对 SBV,从而提高 ECQ。总之,DS 可能导致四川盆地机插秧水稻 ECQ 明显下降。要提高水稻的ECQ,需要在水稻抽穗后将平均温度保持在25.9℃以上。
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Journal of Integrative Agriculture
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