Pub Date : 2024-05-01DOI: 10.1016/j.jia.2024.05.023
Jie Liu, Jie Zhang, Huijuan Yan, Tuyong Yi, Won Bo Shim, Zehua Zhou
{"title":"FvVam6 is associated with fungal development and fumonisin biosynthesis via vacuole morphology regulation in Fusarium verticillioides1","authors":"Jie Liu, Jie Zhang, Huijuan Yan, Tuyong Yi, Won Bo Shim, Zehua Zhou","doi":"10.1016/j.jia.2024.05.023","DOIUrl":"https://doi.org/10.1016/j.jia.2024.05.023","url":null,"abstract":"","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141042713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-22DOI: 10.1016/j.jia.2024.03.066
Qian Chen, Shunyuan Yong, Fan Xu, Hao Fu, Jiangbo Dang, Qiao He, Danlong Jing, Di Wu, Guolu Liang, Qigao Guo
The () gene family is involved in the regulation of gene expression and plant growth, development, and stress responses. To investigate the function of loquat genes in the growth and developmental regulation of plants, a loquat gene homologous to was cloned. expression was induced by gibberellin, and ectopic transgenic plants containing this gene exhibited earlier bloom and longer primary roots since these phenotypic characteristics are related to higher gibberellin content. Transcriptome analysis and qRT-PCR results showed that the expression levels of and which encode key enzymes in gibberellin biosynthesis, were significantly increased. Furthermore, we confirmed that EjGASA6 could promote the expression of the luciferase reporter system. Overall, our results suggest that promotes blooming and main-root elongation by positively regulating gibberellin biosynthesis. These findings broaden our understanding of the role of GASAs in plant development and growth, and lay the groundwork for future research into the functions of in regulating loquat growth and development.
{"title":"EjGASA6 promotes flowering and root elongation by enhancing gibberellin biosynthesis","authors":"Qian Chen, Shunyuan Yong, Fan Xu, Hao Fu, Jiangbo Dang, Qiao He, Danlong Jing, Di Wu, Guolu Liang, Qigao Guo","doi":"10.1016/j.jia.2024.03.066","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.066","url":null,"abstract":"The () gene family is involved in the regulation of gene expression and plant growth, development, and stress responses. To investigate the function of loquat genes in the growth and developmental regulation of plants, a loquat gene homologous to was cloned. expression was induced by gibberellin, and ectopic transgenic plants containing this gene exhibited earlier bloom and longer primary roots since these phenotypic characteristics are related to higher gibberellin content. Transcriptome analysis and qRT-PCR results showed that the expression levels of and which encode key enzymes in gibberellin biosynthesis, were significantly increased. Furthermore, we confirmed that EjGASA6 could promote the expression of the luciferase reporter system. Overall, our results suggest that promotes blooming and main-root elongation by positively regulating gibberellin biosynthesis. These findings broaden our understanding of the role of GASAs in plant development and growth, and lay the groundwork for future research into the functions of in regulating loquat growth and development.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140830323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-22DOI: 10.1016/j.jia.2024.03.064
Xiangyang Zhang, Yumei Zhang, Shenggen Fan
China’s crop structure has undergone significant changes in the last two decades since 2000, with an increase in the share of cereals, vegetables, and fruit, squeezing out other crops. As a result, land productivity, nutrient supply, and carbon emissions have changed. How to reallocate limited farmland among crops to achieve the multiple goals of agrifood systems becomes an important issue. This study explores the sources of land productivity and nutrition supply growth and carbon emissions reduction, and identifies the multiple roles of crop structural change from 2003 to 2020 based on a decomposition analysis. The results reveal that the growth within crops is still the primary driver in land productivity and nutrition supply and the reduction in carbon emissions. However, structural change also plays various roles at different periods. From 2003 to 2010, crop structural change increased the total calorie supply but lowered land productivity and contributed at least 70% of the total growth of carbon emissions. The crop structure was relatively stable, and their effects were modest from 2010 to 2015. From 2015 to 2020, the crop structural change began to play a greater role and generate synergistic effects in improving land productivity, micronutrient supply, and reducing carbon emissions, contributing to approximately a quarter of the growth of land productivity and 30% of total carbon emissions reduction. These results suggest that strategies for crop structural change should comprehensively consider its multiple impacts, aiming to achieve co-benefits while minimizing trade-offs.
{"title":"The multiple roles of crop structural change in productivity, nutrition and environment in China: A decomposition analysis","authors":"Xiangyang Zhang, Yumei Zhang, Shenggen Fan","doi":"10.1016/j.jia.2024.03.064","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.064","url":null,"abstract":"China’s crop structure has undergone significant changes in the last two decades since 2000, with an increase in the share of cereals, vegetables, and fruit, squeezing out other crops. As a result, land productivity, nutrient supply, and carbon emissions have changed. How to reallocate limited farmland among crops to achieve the multiple goals of agrifood systems becomes an important issue. This study explores the sources of land productivity and nutrition supply growth and carbon emissions reduction, and identifies the multiple roles of crop structural change from 2003 to 2020 based on a decomposition analysis. The results reveal that the growth within crops is still the primary driver in land productivity and nutrition supply and the reduction in carbon emissions. However, structural change also plays various roles at different periods. From 2003 to 2010, crop structural change increased the total calorie supply but lowered land productivity and contributed at least 70% of the total growth of carbon emissions. The crop structure was relatively stable, and their effects were modest from 2010 to 2015. From 2015 to 2020, the crop structural change began to play a greater role and generate synergistic effects in improving land productivity, micronutrient supply, and reducing carbon emissions, contributing to approximately a quarter of the growth of land productivity and 30% of total carbon emissions reduction. These results suggest that strategies for crop structural change should comprehensively consider its multiple impacts, aiming to achieve co-benefits while minimizing trade-offs.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140830038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-22DOI: 10.1016/j.jia.2024.03.068
Jialing Fu, Qingjiang Wu, Xia Wang, Juan Sun, Li Liao, Li Li, Qiang Xu
The flesh color of pummelo () fruits is highly diverse and largely depends on the level of carotenoids, which are beneficial to human health. It is vital to investigate the regulatory network of carotenoid biosynthesis to improve the carotenoid content in pummelo. However, the molecular mechanism underlying carotenoid accumulation in pummelo is not fully understood. In this study, we identified a novel histone methyltransferase gene, , involved in carotenoid regulation by analyzing the flesh transcriptome of typical white-fleshed pummelo, red-fleshed pummelo and extreme-colored F hybrids from a segregated pummelo population. Expression of corresponded to flesh color change and was highly coexpressed with . Interestingly, and are located physically adjacent to each other on the chromosome in opposite directions, sharing a partially overlapping promoter region. Subcellular localization analysis indicated that CgSDG40 localizes to the nucleus. Overexpression of significantly increased the total carotenoid content in citrus calli relative to that in wild type. In addition, expression of was significantly activated in overexpression lines relative to wild type. Taken together, our findings reveal a novel histone methyltransferase regulator, CgSDG40, involved in the regulation of carotenoid biosynthesis in citrus and provide new strategies for molecular design breeding and genetic improvement of fruit color and nutritional quality.
{"title":"A novel histone methyltransferase gene CgSDG40 positively regulates carotenoid biosynthesis during citrus fruit ripening","authors":"Jialing Fu, Qingjiang Wu, Xia Wang, Juan Sun, Li Liao, Li Li, Qiang Xu","doi":"10.1016/j.jia.2024.03.068","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.068","url":null,"abstract":"The flesh color of pummelo () fruits is highly diverse and largely depends on the level of carotenoids, which are beneficial to human health. It is vital to investigate the regulatory network of carotenoid biosynthesis to improve the carotenoid content in pummelo. However, the molecular mechanism underlying carotenoid accumulation in pummelo is not fully understood. In this study, we identified a novel histone methyltransferase gene, , involved in carotenoid regulation by analyzing the flesh transcriptome of typical white-fleshed pummelo, red-fleshed pummelo and extreme-colored F hybrids from a segregated pummelo population. Expression of corresponded to flesh color change and was highly coexpressed with . Interestingly, and are located physically adjacent to each other on the chromosome in opposite directions, sharing a partially overlapping promoter region. Subcellular localization analysis indicated that CgSDG40 localizes to the nucleus. Overexpression of significantly increased the total carotenoid content in citrus calli relative to that in wild type. In addition, expression of was significantly activated in overexpression lines relative to wild type. Taken together, our findings reveal a novel histone methyltransferase regulator, CgSDG40, involved in the regulation of carotenoid biosynthesis in citrus and provide new strategies for molecular design breeding and genetic improvement of fruit color and nutritional quality.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141937908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-22DOI: 10.1016/j.jia.2024.03.069
Bo Jiao, Xin Guo, Yiying Chen, Shah Faisal, Wenchao Liu, Xiaojie Ma, Bicong Wu, Guangyue Ren, Qiang Wang
This study develops low-fat microwaved peanut snacks (LMPS) using partially defatted peanuts (PDP) with different defatting ratios, catering to people’s pursuit of healthy, low-fat cuisine. The effects of defatting treatment on the structural characteristics, texture, color, and nutrient composition of LMPS were comprehensively explored. The structural characteristics of LMPS were characterized using X-ray micro-computed tomography (Micro-CT) and scanning electron microscope (SEM). The results demonstrated that the porosity, pore number, pore volume, brightness, brittleness, protein content, and total sugar content of LMPS all significantly increased (<0.05) with the increase in the defatting ratio. At the micro level, porous structure, cell wall rupture, and loss of intracellular material could be observed in LMPS after defatting treatments. LMPS made from PDP with a defatting ratio of 64.44% had the highest internal pore structural parameters (porosity 59%, pore number 85.3×10, pore volume 68.23 mm), the brightest color (L* 78.39±0.39), the best brittleness (3.64±0.21) mm), and the best nutrition (high protein content, (34.02±0.38)%; high total sugar content, (17.45±0.59)%; low-fat content, (27.58±0.85)%). The study provides a theoretical basis for the quality improvement of LMPS.
{"title":"Low-fat microwaved peanut snacks production: Effect of defatting treatment on structural characteristics, texture, color, and nutrition","authors":"Bo Jiao, Xin Guo, Yiying Chen, Shah Faisal, Wenchao Liu, Xiaojie Ma, Bicong Wu, Guangyue Ren, Qiang Wang","doi":"10.1016/j.jia.2024.03.069","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.069","url":null,"abstract":"This study develops low-fat microwaved peanut snacks (LMPS) using partially defatted peanuts (PDP) with different defatting ratios, catering to people’s pursuit of healthy, low-fat cuisine. The effects of defatting treatment on the structural characteristics, texture, color, and nutrient composition of LMPS were comprehensively explored. The structural characteristics of LMPS were characterized using X-ray micro-computed tomography (Micro-CT) and scanning electron microscope (SEM). The results demonstrated that the porosity, pore number, pore volume, brightness, brittleness, protein content, and total sugar content of LMPS all significantly increased (<0.05) with the increase in the defatting ratio. At the micro level, porous structure, cell wall rupture, and loss of intracellular material could be observed in LMPS after defatting treatments. LMPS made from PDP with a defatting ratio of 64.44% had the highest internal pore structural parameters (porosity 59%, pore number 85.3×10, pore volume 68.23 mm), the brightest color (L* 78.39±0.39), the best brittleness (3.64±0.21) mm), and the best nutrition (high protein content, (34.02±0.38)%; high total sugar content, (17.45±0.59)%; low-fat content, (27.58±0.85)%). The study provides a theoretical basis for the quality improvement of LMPS.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141571039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-22DOI: 10.1016/j.jia.2024.03.065
Xiabing Kong, Qiusheng Chen, Min Xu, Yihui Liu, Xiaoming Li, Lingxi Han, Qiang Zhang, Haoliang Wan, Lu Liu, Xubo Zhao, Jiyun Nie
Winter jujube ( ‘Dongzao’) is greatly appreciated by consumers for its excellent quality, but brand infringement frequently occurs in the market. Here, we first determined a total of 38 elements in 167 winter jujube samples from the main winter jujube producing areas of China by inductively coupled plasma mass spectrometer (ICP-MS). As a result, 16 elements (Mg, K, Mn, Cu, Zn, Mo, Ba, Be, As, Se, Cd, Sb, Ce, Er, Tl, and Pb) exhibited significant differences in samples from different producing areas. Supervised linear discriminant analysis (LDA) and orthogonal projection to latent structures discriminant analysis (OPLS-DA) showed better performance in identifying the origin of samples than unsupervised principal component analysis (PCA). LDA and OPLS-DA had a mean identification accuracy of 87.84 and 94.64% in the testing set, respectively. By using the multilayer perceptron (MLP) and C5.0, the prediction accuracy of the models could reach 96.36 and 91.06%, respectively. Based on the above four chemometric methods, Cd, Tl, Mo and Se were selected as the main variables and principal markers for the origin identification of winter jujube. Overall, this study demonstrates that it is practical and precise to identify the origin of winter jujube through multi-element fingerprint analysis with chemometrics, and may also provide reference for establishing the origin traceability system of other fruits.
{"title":"Geographical origin identification of winter jujube (Ziziphus jujuba ‘Dongzao’) by using multi-element fingerprinting with chemometrics","authors":"Xiabing Kong, Qiusheng Chen, Min Xu, Yihui Liu, Xiaoming Li, Lingxi Han, Qiang Zhang, Haoliang Wan, Lu Liu, Xubo Zhao, Jiyun Nie","doi":"10.1016/j.jia.2024.03.065","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.065","url":null,"abstract":"Winter jujube ( ‘Dongzao’) is greatly appreciated by consumers for its excellent quality, but brand infringement frequently occurs in the market. Here, we first determined a total of 38 elements in 167 winter jujube samples from the main winter jujube producing areas of China by inductively coupled plasma mass spectrometer (ICP-MS). As a result, 16 elements (Mg, K, Mn, Cu, Zn, Mo, Ba, Be, As, Se, Cd, Sb, Ce, Er, Tl, and Pb) exhibited significant differences in samples from different producing areas. Supervised linear discriminant analysis (LDA) and orthogonal projection to latent structures discriminant analysis (OPLS-DA) showed better performance in identifying the origin of samples than unsupervised principal component analysis (PCA). LDA and OPLS-DA had a mean identification accuracy of 87.84 and 94.64% in the testing set, respectively. By using the multilayer perceptron (MLP) and C5.0, the prediction accuracy of the models could reach 96.36 and 91.06%, respectively. Based on the above four chemometric methods, Cd, Tl, Mo and Se were selected as the main variables and principal markers for the origin identification of winter jujube. Overall, this study demonstrates that it is practical and precise to identify the origin of winter jujube through multi-element fingerprint analysis with chemometrics, and may also provide reference for establishing the origin traceability system of other fruits.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140830044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-22DOI: 10.1016/j.jia.2024.03.067
Lin Chen, Chao Li, Jiahao Zhang, Zongrui Li, Qi Zeng, Qingguo Sun, Xiaowu Wang, Limin Zhao, Lugang Zhang, Baohua Li
Chinese cabbage is an important leafy vegetable crop with high water demand and susceptibility to drought stress. To explore the molecular mechanisms underlying the response to drought, we performed a transcriptome analysis of drought-tolerant and -sensitive Chinese cabbage genotypes under drought stress, and uncovered core drought-responsive genes and key signaling pathways. A co-expression network was constructed by a weighted gene co-expression network analysis (WGCNA) and candidate hub genes involved in drought tolerance were identified. Furthermore, abscisic acid (ABA) biosynthesis and signaling pathways and their drought responses in Chinese cabbage leaves were systemically explored. We also found that drought treatment increased the antioxidant enzyme activities and glucosinolate contents significantly. These results substantially enhance our understanding of the molecular mechanisms underlying drought responses in Chinese cabbage.
{"title":"Physiological and transcriptome analyses of Chinese cabbage in response to drought stress","authors":"Lin Chen, Chao Li, Jiahao Zhang, Zongrui Li, Qi Zeng, Qingguo Sun, Xiaowu Wang, Limin Zhao, Lugang Zhang, Baohua Li","doi":"10.1016/j.jia.2024.03.067","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.067","url":null,"abstract":"Chinese cabbage is an important leafy vegetable crop with high water demand and susceptibility to drought stress. To explore the molecular mechanisms underlying the response to drought, we performed a transcriptome analysis of drought-tolerant and -sensitive Chinese cabbage genotypes under drought stress, and uncovered core drought-responsive genes and key signaling pathways. A co-expression network was constructed by a weighted gene co-expression network analysis (WGCNA) and candidate hub genes involved in drought tolerance were identified. Furthermore, abscisic acid (ABA) biosynthesis and signaling pathways and their drought responses in Chinese cabbage leaves were systemically explored. We also found that drought treatment increased the antioxidant enzyme activities and glucosinolate contents significantly. These results substantially enhance our understanding of the molecular mechanisms underlying drought responses in Chinese cabbage.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141571040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-18DOI: 10.1016/j.jia.2024.03.057
Lingling Li, Junhong Fu, Changgeng Dai, Yuhang Zhou, Yang Hu, Hongbo Li
Microvitellogenin (mVg) belongs to the lepidopteran-specific lipoprotein_11 super family and is thought to be involved in insect reproduction. However, the function of mVg in reproduction has not been established. In this study, we identified and characterized in , a destructive insect pest of grain crops. is comprised of a 1,310 bp ORF encoding 437 amino acids. Structural analysis showed that MsmVg contains three conserved domains in the lipoprotein_11 super family, including the all-α-N-terminal domain (NTD), a homologous domain (PPD), and an all-β-C-terminal domain (CTD). was highly expressed in mature female adults and enriched in the ovaries of three-day-old female adults. Disruption of by RNA interference did not change the expression of , vitellogenin () or the vitellogenin receptor () in fat bodies, but it inhibited their transcription in ovaries. Phenotype analysis showed that knockdown of did not affect yolk deposition in ovaries, but impaired eggshell formation and led to a reduction in the reproductive capacity. Furthermore, knockdown of significantly inhibited the expression of genes associated with eggshell formation ( and ). Taken together, these data suggest that plays an important role in female reproduction by affecting eggshell formation in . , which provides a potential target for managing this insect pest.
{"title":"Disruption of a microvitellogenin gene impairs eggshell formation in Mythimna separata","authors":"Lingling Li, Junhong Fu, Changgeng Dai, Yuhang Zhou, Yang Hu, Hongbo Li","doi":"10.1016/j.jia.2024.03.057","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.057","url":null,"abstract":"Microvitellogenin (mVg) belongs to the lepidopteran-specific lipoprotein_11 super family and is thought to be involved in insect reproduction. However, the function of mVg in reproduction has not been established. In this study, we identified and characterized in , a destructive insect pest of grain crops. is comprised of a 1,310 bp ORF encoding 437 amino acids. Structural analysis showed that MsmVg contains three conserved domains in the lipoprotein_11 super family, including the all-α-N-terminal domain (NTD), a homologous domain (PPD), and an all-β-C-terminal domain (CTD). was highly expressed in mature female adults and enriched in the ovaries of three-day-old female adults. Disruption of by RNA interference did not change the expression of , vitellogenin () or the vitellogenin receptor () in fat bodies, but it inhibited their transcription in ovaries. Phenotype analysis showed that knockdown of did not affect yolk deposition in ovaries, but impaired eggshell formation and led to a reduction in the reproductive capacity. Furthermore, knockdown of significantly inhibited the expression of genes associated with eggshell formation ( and ). Taken together, these data suggest that plays an important role in female reproduction by affecting eggshell formation in . , which provides a potential target for managing this insect pest.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rice blast, caused by ,is a fungal disease that causes devastating damage to rice production worldwide. During infection, pathogens secrete effector proteins that modulate plant immunity. Disulfide bond formation catalyzed by protein disulfide isomerases (PDI) is essential for protein folding and maturation. However, the biological function of Pdi1 in has not yet been characterized. In this study, we identified the endoplasmic reticulum (ER)-located protein, MoPdi1, in . MoPdi1 regulates conidiation, cell wall stress, and pathogenicity of . Furthermore, the CGHC active sites in the a and a’ redox domain of MoPdi1 were essential for the biological function of . Further tests demonstrated that MoPdi1 was involved in the regulation of ER stress and positively regulated ER phagy. We also found that MoPdi1 interacted with MoHut1. Deletion of led to the bereft of MoHut1 dimerization, which depends on the formation of disulfide bonds. In addition, MoPdi1 affected the normal secretion of the cytoplasmic effector AVR-Pia. We provided evidence that MoHut1 is important for the vegetative growth, conidiation, and pathogenicity in . Therefore, our findings could provide a suitable target point for designing antifungal agrochemicals against rice blast fungus.
稻瘟病是一种真菌病害，对全球水稻生产造成毁灭性破坏。在感染过程中，病原体会分泌调节植物免疫力的效应蛋白。蛋白质二硫键异构酶（PDI）催化的二硫键形成对蛋白质折叠和成熟至关重要。然而，Pdi1 的生物功能尚未确定。在这项研究中，我们发现了内质网（ER）定位蛋白 MoPdi1。此外，MoPdi1 的 a 和 a'氧化还原结构域中的 CGHC 活性位点对其生物功能至关重要。 进一步的试验证明，MoPdi1 参与了 ER 应激的调控，并对 ER 吞噬作用有正向调控作用。我们还发现 MoPdi1 与 MoHut1 相互作用。缺失MoPdi1会导致MoHut1失去二聚化，而二聚化依赖于二硫键的形成。此外，MoPdi1 还影响细胞质效应物 AVR-Pia 的正常分泌。我们提供的证据表明，MoHut1 对疟原虫的无性生长、分生孢子和致病性都很重要。因此，我们的发现可为设计抗稻瘟病真菌的农用化学品提供一个合适的靶点。
{"title":"Protein disulfide isomerase MoPdi1 regulates fungal development, virulence, and endoplasmic reticulum homeostasis in Magnaporthe oryzae1","authors":"Yu Wang, Xiaoru Kang, Xinyue Cui, Jinmei Hu, Yuemin Pan, Yizhen Deng, Shulin Zhang","doi":"10.1016/j.jia.2024.03.054","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.054","url":null,"abstract":"Rice blast, caused by ,is a fungal disease that causes devastating damage to rice production worldwide. During infection, pathogens secrete effector proteins that modulate plant immunity. Disulfide bond formation catalyzed by protein disulfide isomerases (PDI) is essential for protein folding and maturation. However, the biological function of Pdi1 in has not yet been characterized. In this study, we identified the endoplasmic reticulum (ER)-located protein, MoPdi1, in . MoPdi1 regulates conidiation, cell wall stress, and pathogenicity of . Furthermore, the CGHC active sites in the a and a’ redox domain of MoPdi1 were essential for the biological function of . Further tests demonstrated that MoPdi1 was involved in the regulation of ER stress and positively regulated ER phagy. We also found that MoPdi1 interacted with MoHut1. Deletion of led to the bereft of MoHut1 dimerization, which depends on the formation of disulfide bonds. In addition, MoPdi1 affected the normal secretion of the cytoplasmic effector AVR-Pia. We provided evidence that MoHut1 is important for the vegetative growth, conidiation, and pathogenicity in . Therefore, our findings could provide a suitable target point for designing antifungal agrochemicals against rice blast fungus.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Citrus yellow vein clearing virus (CYVCV) is a new citrus virus that has become an important factor restricting the development of China'scitrus industry, and the CYVCV coat protein (CP) is associated with viral pathogenicity. In this study, the Eureka lemon zinc finger protein (ZFP) ClDOF3.4 was shown to interact with CYVCV CP and . Transient expression of in Eureka lemon induced the expression of salicylic acid (SA)-related and hypersensitive response marker genes, and triggered a reactive oxygen species burst, ion leakage necrosis, and the accumulation of free SA. Furthermore, the CYVCV titer in transgenic Eureka lemon plants was approximately 69.4% that in control plants 6 months after inoculation, withonly mild leaf chlorotic spots observed in those transgenic plants. Taken together, the results indicate that ClDOF3.4 not only interacts with CP but also induces an immune response in Eureka lemon by inducing the SA pathways. This is the first report that ZFP is involved in the immune response of a citrus viral disease, which provides a basis for further study of the molecular mechanism of CYVCV infection.
{"title":"Eureka lemon zinc finger protein ClDOF3.4 interacts with citrus yellow vein clearing virus coat protein to inhibit viral infection1","authors":"Ping Liao, Ting Zeng, Mengyang Huangfu, Cairong Zheng, Jiequn Ren, Changyong Zhou, Yan Zhou","doi":"10.1016/j.jia.2024.03.049","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.049","url":null,"abstract":"Citrus yellow vein clearing virus (CYVCV) is a new citrus virus that has become an important factor restricting the development of China'scitrus industry, and the CYVCV coat protein (CP) is associated with viral pathogenicity. In this study, the Eureka lemon zinc finger protein (ZFP) ClDOF3.4 was shown to interact with CYVCV CP and . Transient expression of in Eureka lemon induced the expression of salicylic acid (SA)-related and hypersensitive response marker genes, and triggered a reactive oxygen species burst, ion leakage necrosis, and the accumulation of free SA. Furthermore, the CYVCV titer in transgenic Eureka lemon plants was approximately 69.4% that in control plants 6 months after inoculation, withonly mild leaf chlorotic spots observed in those transgenic plants. Taken together, the results indicate that ClDOF3.4 not only interacts with CP but also induces an immune response in Eureka lemon by inducing the SA pathways. This is the first report that ZFP is involved in the immune response of a citrus viral disease, which provides a basis for further study of the molecular mechanism of CYVCV infection.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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