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A study of stromal collagen in oral lichen planus, carcinoma in situ, early invasive squamous cell carcinoma, and normal mucosa using picrosirius red stain 小天狼星红染色法研究口腔扁平苔藓、原位癌、早期浸润性鳞状细胞癌和正常黏膜的间质胶原
Q3 Medicine Pub Date : 2023-07-01 DOI: 10.4103/jmau.jmau_83_21
D. Nandini, V. Ramya, Vikram S. Amberkar, K. M. Mohan Kumar, G. Madhushankari
Background and Objectives: Oral lichen planus (OLP) is a chronic disease of uncertain cause commonly affecting oral cavity. Although the WHO has designated OLP as a “potentially malignant disorder,” controversies exist regarding its malignant potential. Collagen forms the principal component of stroma or extracellular matrix and its role in carcinogenesis is widely studied in other premalignancies. Although collagen at the basal complex of OLP is widely explored, studies on collagen in the connective tissue stroma are not reported to date. We aimed to observe the nature of collagen in connective tissue stroma of OLP using picrosirius red stain (PSR) under polarized microscope and compare with buccal mucosa without any pathology related to exposure to tobacco and other oral carcinogens, carcinoma in situ (Ca in situ), and early invasive squamous cell carcinoma (EISCC). Materials and Methods: Eighty samples were observed, with twenty samples in each study group. Two 4–6-μ thick sections were obtained from the archival blocks. One section was stained with hematoxylin and eosin for confirming the diagnosis, whereas PSR staining was done for the other section. Both sections were analyzed using a polarizing microscope for evaluating the polarization colors of collagen. The images captured were stored on a computer. Five nonoverlapping fields were selected from each section in all groups and the thickness of five collagen fibers from each section was measured in microns using image analysis software and the polarizing color was also noted. The values obtained were compared using Kruskal–Wallis H-test and Chi-square test. We also used Mann–Whitney U-test for intergroup comparison. Results: The mean width of thick as well as thin fibers was more in controls than Ca in situ, OLP, and EISCC in decreasing order. Mature fibers were predominant in the controls than Ca in situ, OLP, and EISCC in decreasing order. Immature fibers were predominant in EISCC, followed by OLP, Ca in situ, and controls. Comparison of collagen in OLP and Ca in situ showed no statistically significant result in terms of thickness and polarization colors confirming a similarity in the nature of collagen in these two lesions. Conclusion: The stromal collagen of OLP was comparable to Ca in situ suggesting a change in the structure and organization of collagen probably attributed to the role of inflammatory mediators. A study with bigger sample size is recommended to evaluate the role of collagen in malignant transformation of OLP.
背景与目的:口腔扁平苔藓(OLP)是一种病因不明的常见于口腔的慢性疾病。虽然世界卫生组织将OLP指定为“潜在的恶性疾病”,但围绕其恶性可能性存在争议。胶原蛋白是间质或细胞外基质的主要成分,其在其他恶性肿瘤发生中的作用已被广泛研究。虽然OLP基底复合物中的胶原蛋白被广泛研究,但结缔组织基质中胶原蛋白的研究迄今尚未报道。我们在偏光显微镜下使用微天狼星红染色(PSR)观察OLP结缔组织间质胶原的性质,并与未接触烟草等口腔致癌物、原位癌(Ca in situ)和早期侵袭性鳞状细胞癌(EISCC)的口腔黏膜进行比较。材料与方法:共观察80例样本,每组20例。从档案块中取2张4 ~ 6 μ厚的切片。切片用苏木精和伊红染色以确定诊断,另切片用PSR染色。用偏光显微镜分析两个切片,评价胶原蛋白的偏振色。捕获的图像存储在计算机上。在所有组的每个切片中选择5个不重叠的场,使用图像分析软件测量每个切片中5个胶原纤维的厚度,以微米为单位,并记录偏振色。采用Kruskal-Wallis h检验和卡方检验比较所得值。我们还使用Mann-Whitney u检验进行组间比较。结果:对照组粗纤维和细纤维的平均宽度依次大于原位Ca、OLP和EISCC。成熟纤维在对照中以原位Ca、OLP和EISCC为主。未成熟纤维在EISCC中占主导地位,其次是OLP、原位Ca和对照。OLP和Ca原位的胶原比较,在厚度和极化颜色方面没有统计学意义,证实了这两个病变中胶原质的相似性。结论:OLP的基质胶原与Ca的原位相似,表明胶原结构和组织的改变可能与炎症介质的作用有关。建议进行更大样本量的研究来评估胶原蛋白在OLP恶性转化中的作用。
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引用次数: 0
Multifocal unilateral wilms' tumor: Radiopathological correlation 多灶单侧肾母细胞瘤:放射病理学相关性
Q3 Medicine Pub Date : 2023-07-01 DOI: 10.4103/jmau.jmau_121_20
Ravikanth Reddy
Wilms' tumor constitutes majority of malignant primary renal tumors in children. Outcomes of treatment are excellent in Wilms' tumor, with a cumulative survival rate of 87%. A combination of imaging and histopathology is needed for confirmation of Wilms' tumor. However, imaging alone can be used for screening, diagnostic workup, assessment of response to treatment, preoperative evaluation, and follow-up for recurrence. In malignant primary tumors such as Wilms' tumor, where the outcomes following treatment are excellent, management strategies are aimed at risk-based stratification approach, thereby improving the quality of life in children with high-grade tumors while maintaining extremely good outcomes in the pediatric age group with low-grade tumors. This case report describes a rare sonological appearance of multifocal unilateral Wilms' tumor in a 4-month-old infant.
肾母细胞瘤是儿童原发性恶性肾肿瘤的主要组成部分。Wilms肿瘤的治疗结果非常好,累计生存率为87%。影像学和组织病理学的结合是确认Wilms肿瘤的必要条件。然而,单独成像可用于筛查、诊断检查、治疗反应评估、术前评估和复发随访。对于恶性原发肿瘤,如Wilms肿瘤,其治疗后的结果非常好,管理策略旨在基于风险的分层方法,从而提高高级别肿瘤儿童的生活质量,同时保持儿童年龄组低级别肿瘤的极好结果。这个病例报告描述了一个罕见的超声表现多灶单侧Wilms肿瘤的4个月大的婴儿。
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引用次数: 0
Histological Changes in the Background Renal Parenchyma in Neoplastic Nephrectomies and Nephroureterectomy: A 10-Year Single-Center Experience. 肿瘤肾切除术和肾输尿管切除术中肾实质的组织学改变:一项10年单中心研究。
Q3 Medicine Pub Date : 2023-04-01 DOI: 10.4103/jmau.jmau_87_21
Reem A Al Zahrani

Background: Nephrectomies and nephroureterectomies specimen with neoplastic diagnosis provide a great opportunity to examine the background kidney parenchyma. This will help in exploring any microscopic abnormality that may affect the functional capacity of the residual renal tissue and assess the possible need for nephrologist intervention in maximizing the function of the residual renal parenchyma.

Aim: Evaluation of this part of the kidney is overlooked in different centers around the world.

Method: A total of 124 specimens of nephrectomies and nephroureterectomies performed at King Abdulaziz University Hospital between January 2010 and December 2019 were reviewed. The microscopic findings in the nonneoplatic parenchyma were documented. Furthermore, the extent to which these findings were initially identified, investigated, and reported was measured.

Results: Hypertensive and diabetic nephropathy were among the most common findings in the diseased kidney. The nonneoplastic kidney was well sampled in 95% of the cases and mentioned in the report in 25% of the cases.

Conclusion: Although, the evaluation of this part of the kidney is essential, and of great help for the patient prognosis, and quality of kidney function, it is overlooked in different centers around the world, and the causes behind this requires further exploration.

背景:肿瘤诊断的肾切除术和肾输尿管切除术标本为检查肾实质提供了很好的机会。这将有助于发现任何可能影响残留肾组织功能的微观异常,并评估是否需要肾病专家干预,以最大限度地发挥残留肾实质的功能。目的:在世界各地的不同中心,对肾脏这部分的评估被忽视了。方法:回顾2010年1月至2019年12月在阿卜杜勒阿齐兹国王大学医院进行的124例肾切除术和肾输尿管切除术标本。非肿瘤实质的显微镜检查结果被记录下来。此外,还测量了这些发现最初被确定、调查和报告的程度。结果:高血压和糖尿病肾病是病变肾脏中最常见的表现。非肿瘤性肾脏在95%的病例中被充分取样,报告中提到的病例占25%。结论:虽然对这部分肾脏的评估是必不可少的,对患者的预后和肾功能质量有很大的帮助,但在世界各地的不同中心都被忽视了,其背后的原因有待进一步探讨。
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引用次数: 0
Ectopic Adrenal Rest in Paratubal Region: A Rare Lesion in a Rare Location. 肾上腺旁区异位休息:罕见部位的罕见病变。
Q3 Medicine Pub Date : 2023-04-01 DOI: 10.4103/jmau.jmau_139_20
Indranil Chakrabarti, Nabanita Das

Ectopic adrenal rests (EAT) are rare lesions comprising predominantly adrenocortical tissue in various locations. Here, we report a case of EAT in the paratubal location which was incidentally detected in a salpingo-oophorectomy specimen from a 21-year-old female. The identification of these lesions is extremely essential as it can be associated with hyperplasia and neoplasia.

异位肾上腺休息(EAT)是一种罕见的病变,主要包括不同部位的肾上腺皮质组织。在这里,我们报告一个病例在输卵管旁的位置,偶然发现的输卵管卵巢切除术标本从一个21岁的女性。这些病变的识别是非常必要的,因为它可能与增生和瘤变有关。
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引用次数: 0
Expression of p53 as Potential Biomarker in Oral Submucous Fibrosis: An Immunohistochemical Study. p53作为口腔黏膜下纤维化潜在生物标志物的表达:一项免疫组织化学研究。
Q3 Medicine Pub Date : 2023-04-01 DOI: 10.4103/jmau.jmau_23_21
K A Kamala, Sujata R Kanetkar, Kailash D Datkhile, S Sankethguddad

Background: Oral submucous fibrosis (OSMF) is potentially malignant disorder known to transform into oral cancer. The malignant transformation is often associated with changes at the genetic level that in turn is reflected by the altered expression of proteins related to cell cycle, proliferation, and apoptosis. Expression of p53 tumor suppressor gene is one of the common findings in human cancers including the oral cancer. Therefore, the early detection of potentially malignant OSMF has been crucial in the inhibition of oral cancer.

Aim and objectives: To determine the main pathological logical factors and expression of aberrant p53 in OSMF, oral squamous cell carcinomas (OSCC) and in normal patients, to study correlation between p53 expression with clinical staging and histological grading of OSMF.

Materials and methods: An immunohistochemical (IHC) study was performed for p53 expression on 35 cases of OSMF, 10 cases of OSCC with history of habits and 10 normal patients without any habits.

Results: The expression of p53 showed a significant difference between normal oral mucosa, OSMF and OSCC samples.

Conclusion: The study demonstrated a high incidence of p53 over expression in OSMF and OSCC. The results indicate that p53 over expression may play a role in pathogenesis of OSMF and in the development of Oral squamous cell carcinoma. With early detection of the high-risk patients with OSMF, we can expect to develop more intensive treatment modalities, leading to the reduction in cancer transformation rate from OSMF.

背景:口腔黏膜下纤维化(OSMF)是一种已知可转化为口腔癌的潜在恶性疾病。恶性转化通常与遗传水平的改变有关,而遗传水平的改变又反映在与细胞周期、增殖和凋亡相关的蛋白质表达的改变上。p53肿瘤抑制基因的表达是包括口腔癌在内的人类癌症的常见发现之一。因此,早期发现潜在的恶性OSMF对抑制口腔癌至关重要。目的:确定OSMF、口腔鳞癌(OSCC)及正常患者中p53异常的主要病理因素及表达,研究p53表达与OSMF临床分期及组织学分级的相关性。材料与方法:应用免疫组化(IHC)方法对35例OSMF、10例有生活习惯的OSCC和10例无生活习惯的正常患者的p53表达进行研究。结果:p53在正常口腔黏膜、OSMF和OSCC样品中的表达差异有统计学意义。结论:本研究表明p53在OSMF和OSCC中高表达。提示p53过表达可能参与了OSMF的发病和口腔鳞状细胞癌的发生发展。随着OSMF高危患者的早期发现,我们有望开发出更强化的治疗方式,从而降低OSMF的癌症转换率。
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引用次数: 0
Preliminary Report on Histopathology of Odontogenic Keratocyst in Third Dimension: Three-Dimensional Video and Images. 牙源性角化囊肿的三维组织病理学初步报告:三维视频和图像。
Q3 Medicine Pub Date : 2023-04-01 DOI: 10.4103/jmau.jmau_1_21
Sandhya Tamgadge, Avinash Tamgadge

Introduction: Odontogenic keratocyst (OKC) is a developmental odontogenic cyst of the jaws that causes extensive destruction even before the clinical manifestations are evident. It is a well-known entity among private practitioners as it has a high recurrence rate and requires meticulous treatment planning. Unfortunately, clinicians have less understood its basic histopathology.

Objective: This article highlights the preliminary attempt of an author in designing third dimension of histopathological aspect of OKC so that it is easily understood by oral pathologists, general pathologists, nonpathologists, students, and even patients too.

Methodology: Preliminary three-dimensional (3D) images and videos on histopathological aspect of OKC were designed by the author using 3DMax (Autodesk Media and Entertainment) and Adobe Premiere Pro 5.5 software which is a video editing software (Adobe Systems) based on two-dimensional descriptions mentioned in the literature.

Results: Internal cellular architecture of OKC was created in a lifelike manner through 3D animation video and images.

Conclusion: Histopathological aspect of all oral lesions should be explained in third dimension too.

牙源性角化囊肿(OKC)是一种颌骨发育性牙源性囊肿,在临床表现明显之前就会引起广泛的破坏。由于复发率高,需要精心的治疗计划,在私人医生中是一个众所周知的实体。不幸的是,临床医生对其基本的组织病理学了解较少。目的:本文重点介绍了作者在OKC组织病理学方面的三维设计的初步尝试,以便口腔病理学家、普通病理学家、非病理学家、学生甚至患者也能很容易地理解。方法:根据文献中二维描述,作者使用3DMax (Autodesk Media and Entertainment)软件和Adobe Premiere Pro 5.5视频编辑软件(Adobe Systems)软件设计OKC组织病理学方面的初步三维(3D)图像和视频。结果:通过三维动画视频和图像逼真地描绘了OKC的内部细胞结构。结论:所有口腔病变的组织病理学方面也应从三维角度进行解释。
{"title":"Preliminary Report on Histopathology of Odontogenic Keratocyst in Third Dimension: Three-Dimensional Video and Images.","authors":"Sandhya Tamgadge,&nbsp;Avinash Tamgadge","doi":"10.4103/jmau.jmau_1_21","DOIUrl":"https://doi.org/10.4103/jmau.jmau_1_21","url":null,"abstract":"<p><strong>Introduction: </strong>Odontogenic keratocyst (OKC) is a developmental odontogenic cyst of the jaws that causes extensive destruction even before the clinical manifestations are evident. It is a well-known entity among private practitioners as it has a high recurrence rate and requires meticulous treatment planning. Unfortunately, clinicians have less understood its basic histopathology.</p><p><strong>Objective: </strong>This article highlights the preliminary attempt of an author in designing third dimension of histopathological aspect of OKC so that it is easily understood by oral pathologists, general pathologists, nonpathologists, students, and even patients too.</p><p><strong>Methodology: </strong>Preliminary three-dimensional (3D) images and videos on histopathological aspect of OKC were designed by the author using 3DMax (Autodesk Media and Entertainment) and Adobe Premiere Pro 5.5 software which is a video editing software (Adobe Systems) based on two-dimensional descriptions mentioned in the literature.</p><p><strong>Results: </strong>Internal cellular architecture of OKC was created in a lifelike manner through 3D animation video and images.</p><p><strong>Conclusion: </strong>Histopathological aspect of all oral lesions should be explained in third dimension too.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"121-123"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5e/a4/JMAU-11-121.PMC10337673.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9825868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Light Microscopy and Ultrastructure of Body Wall in Leech Haemadipsa zeylanica. 水蛭体壁光镜及超微结构研究。
Q3 Medicine Pub Date : 2023-04-01 DOI: 10.4103/jmau.jmau_45_22
Usha Anilkumar, Anita S Jadhav

Background information: Leeches are widely distributed worldwide in a diversity of habitats, such as freshwater, seas, desert, and oases. Since literature does not have an elaborate study on histology of leeches, thus emphasis is given to study the histology.

Aim: The aim of this study is to examine the histology of free-living leeches Haemadipsa zeylanica under light microscopy and extended to ultrastructural study under transmission electron microscopy.

Materials and methods: Tissue was fixed in 10% formalin, dehydrated with alcohol, infiltrated and embedded in wax, and thin section was cut using a rotary microtome. Sections were stained with hematoxylin and eosin.

Results: The epithelial layer of cells in H. zeylanica had a thin layer of cuticles covering it. In light microscopy, the epidermis has three types of epidermal cells, namely, glandular cells (Type I, Type II, and Type III), supporting cells (Type IV), and pigment cells (Type V). When the three glandular cells of the body wall of H. zeylanica epidermis were examined under an electron microscope, three types of distinct electron-dense granules were identified. Type I cells with granules, Type II cells with course granules close to the dermis were identified as pear-shaped secretory cells, and Type III cells with electron-dense granules.

背景资料:水蛭在世界范围内广泛分布于淡水、海洋、沙漠和绿洲等多种栖息地。由于文献没有对水蛭的组织学进行详细的研究,因此重点放在对水蛭的组织学研究上。目的:在光镜下观察自由生活水蛭的组织结构,并扩展到透射电镜下的超微结构研究。材料和方法:将组织用10%福尔马林固定,用酒精脱水,用蜡浸润包埋,用旋转切片机切片。切片用苏木精和伊红染色。结果:黑麦细胞上皮层有一层薄薄的角质层覆盖。光镜下,表皮有三种类型的表皮细胞,分别是腺细胞(I型、II型和III型)、支持细胞(IV型)和色素细胞(V型)。当在电镜下观察泽兰表皮体壁的三种腺细胞时,发现了三种不同类型的电子致密颗粒。带颗粒的I型细胞和靠近真皮层的带过程颗粒的II型细胞被鉴定为梨形分泌细胞,带电子致密颗粒的III型细胞被鉴定为梨形分泌细胞。
{"title":"Light Microscopy and Ultrastructure of Body Wall in Leech <i>Haemadipsa zeylanica</i>.","authors":"Usha Anilkumar,&nbsp;Anita S Jadhav","doi":"10.4103/jmau.jmau_45_22","DOIUrl":"https://doi.org/10.4103/jmau.jmau_45_22","url":null,"abstract":"<p><strong>Background information: </strong>Leeches are widely distributed worldwide in a diversity of habitats, such as freshwater, seas, desert, and oases. Since literature does not have an elaborate study on histology of leeches, thus emphasis is given to study the histology.</p><p><strong>Aim: </strong>The aim of this study is to examine the histology of free-living leeches <i>Haemadipsa</i> <i>zeylanica</i> under light microscopy and extended to ultrastructural study under transmission electron microscopy.</p><p><strong>Materials and methods: </strong>Tissue was fixed in 10% formalin, dehydrated with alcohol, infiltrated and embedded in wax, and thin section was cut using a rotary microtome. Sections were stained with hematoxylin and eosin.</p><p><strong>Results: </strong>The epithelial layer of cells in <i>H. zeylanica</i> had a thin layer of cuticles covering it. In light microscopy, the epidermis has three types of epidermal cells, namely, glandular cells (Type I, Type II, and Type III), supporting cells (Type IV), and pigment cells (Type V). When the three glandular cells of the body wall of <i>H. zeylanica</i> epidermis were examined under an electron microscope, three types of distinct electron-dense granules were identified. Type I cells with granules, Type II cells with course granules close to the dermis were identified as pear-shaped secretory cells, and Type III cells with electron-dense granules.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"81-86"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f4/f8/JMAU-11-81.PMC10337674.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Placental Dysfunction and Acute Toxoplasmosis: The Role of Melatonin in Relation to Inflammatory Cytokines Interleukin-10 and Interleukin-12. 胎盘功能障碍和急性弓形虫病:褪黑素在炎症细胞因子白介素-10和白介素-12中的作用。
Q3 Medicine Pub Date : 2023-04-01 DOI: 10.4103/jmau.jmau_122_20
Azhar H Al-Kuraishi, Hatham Ibraheem Khalil, Huda Hameed Hassan, Hayder M Al-Kuraishy
Objective: The objective of this study was to elucidate the potential role of anti-inflammatory interleukin (IL)-10 and pro-inflammatory (IL-12) cytokines as well as melatonin (MEL) in the development of placental dysfunction in the pregnant women with acquired toxoplasmosis (TOX). Materials and Methods: This case–control study was carried out at the Department of Clinical Parasitology in teamwork with the Department of Gynecology and Obstetrics, Al-Yarmouk Teaching Hospital, College of Medicine, Al-Mustansiriyah University, Baghdad, Iraq, from September 2018 to February 2019. The recruited patients and healthy controls were allocated into two groups – Group A: pregnant women with acute TOX (n = 45) and Group B: healthy pregnant women (n = 25). Anti-Toxoplasma (Toxo) immunoglobulin M (IgM), serum and placental as well as serum–placental (SP) ratio of MEL, IL-10, and IL-12 were measured. SPSS version 20.00 was used for data analysis. Results: Anti-Toxo IgM serum level and IL-12 serum levels were higher compared with controls (P = 0.001). Both MEL and IL-10 serum levels were lower in the pregnant women with acute TOX compared with controls (P = 0.002 and P = 0.002), respectively. Besides, Both MEL and IL-10 placental levels were lower in the pregnant women with acute TOX compared with controls (P = 0.001). As well, placental IL-12 level was higher in the pregnant women with acute TOX compared with controls (P = 0.001). SP ratio of MEL was higher in the pregnant women with acute TOX compared with controls (P = 0.001). Conclusion: MEL and anti-inflammatory IL-10 are reduced in the pregnant women with acute TOX, while IL-12 is increased. SP ratio of MEL but not of IL-10 or IL-12 is elevated in the pregnant women with acute TOX reflecting the risk of PD.
目的:探讨抗炎白细胞介素(IL -10)和促炎细胞因子(IL-12)以及褪黑素(MEL)在获得性弓形虫病(TOX)孕妇胎盘功能障碍发生中的潜在作用。材料与方法:本病例对照研究于2018年9月至2019年2月在伊拉克巴格达Al-Mustansiriyah大学医学院Al-Yarmouk教学医院临床寄生虫科与妇产科合作开展。将招募的患者和健康对照者分为两组:A组:急性TOX孕妇(n = 45)和B组:健康孕妇(n = 25)。测定抗弓形虫(Toxo)免疫球蛋白M (IgM)、血清和胎盘及血清-胎盘(SP)比值MEL、IL-10、IL-12。采用SPSS 20.00版本进行数据分析。结果:血清抗弓形虫IgM和IL-12水平高于对照组(P = 0.001)。急性TOX孕妇血清MEL和IL-10水平均低于对照组(P = 0.002和P = 0.002)。此外,急性TOX孕妇胎盘MEL和IL-10水平均低于对照组(P = 0.001)。急性TOX孕妇胎盘IL-12水平高于对照组(P = 0.001)。急性TOX孕妇MEL的SP比值高于对照组(P = 0.001)。结论:急性TOX孕妇血清MEL及抗炎IL-10降低,IL-12升高。急性TOX孕妇MEL的SP比值升高,但IL-10和IL-12的SP比值未升高,反映PD的危险。
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引用次数: 1
Epidemiological, Clinical, and Histopathological Features of Salivary Gland Tumors among 150 Sudanese Patients: 10 Years' Experience. 150名苏丹患者唾液腺肿瘤的流行病学、临床和组织病理学特征:10年的经验
Q3 Medicine Pub Date : 2023-04-01 DOI: 10.4103/jmau.jmau_113_20
Somaya T S Hamid, Mohammed A M Abdalla, Azza A Zulfu, Azza Ramadan, Musaab Ahmed, Mohamed H Ahmed

Background and objectives: Salivary gland tumors (SGTs) are serious challenges to pathologists. Herein, we aimed to assess epidemiological and histopathological characteristics of SGTs among Sudanese patients.

Materials and methods: This retrospective descriptive study was undertaken at The pathology department in Khartoum State between 2008 and 2018. Patient records, histopathological reports, and slides were retrieved; and re-examined by two histopathologists. Diagnoses were reclassified according to the 2017 WHO classification of SGTs.

Results: Overall, 150 cases of Sudanese patients with SGT were included (90 [60%] males and 60 [40%] females). Among these, 105 were benign (70%) and 45 were malignant (30%). The parotid glands were the most common site for both benign and malignant tumors (77/150; 51%: 59 benign (76.6%) and 18 malignant [23.4%]). The next common site was the submandibular gland (54 [36%]: 38 benign [70.3%] and 16 malignant [29.7%]), followed by minor salivary glands (19 [12.7%]: 8 benign and 11 malignant [57.9%]). Benign gland entities included pleomorphic adenoma (88/105; 83.7%), oncocytoma (5/105; 4.8%), myoepithelioma (4/105; 3.8%), Whartin tumors (3/105; 2.9%), basal cell adenoma (3/105; 2.9%), and sialolipoma (2/105; 1.9%). Malignant gland entities included adenoid cystic carcinoma (12; 26.7%), mucoepidermoid carcinoma (10; 22,2%), acinic cell carcinoma (6; 13.3%), poorly differentiated carcinoma (4; 8.9%), adenocarcinoma NOS (not otherwise specified) (4; 8.9%), basal cell adenocarcinoma (3; 6.7%), carcinoma ex pleomorphic adenoma (3; 6.7%), polymorphous adenocarcinoma (2; 4.4%), salivary duct carcinoma (1; 2.2%), and epithelial-myoepithelial carcinoma (2.2%).

Conclusions: SGTs shared several epidemiological and histopathological features, exhibiting high incidence in the parotid and submandibular glands, lower prevalence in minor glands, and greater male predominance.

背景与目的:唾液腺肿瘤(sgt)是病理学家面临的严峻挑战。在此,我们旨在评估苏丹患者中sgt的流行病学和组织病理学特征。材料和方法:本回顾性描述性研究于2008年至2018年在喀土穆州病理学部门进行。检索患者记录、组织病理学报告和载玻片;并由两名组织病理学家重新检查。根据2017年世卫组织sgt分类对诊断进行了重新分类。结果:总共纳入150例苏丹SGT患者(90例[60%]男性,60例[40%]女性)。其中良性105例(70%),恶性45例(30%)。腮腺是良恶性肿瘤最常见的部位(77/150;51%:良性59例(76.6%),恶性18例(23.4%)。其次是颌下腺(54例[36%]:良性38例[70.3%],恶性16例[29.7%]),其次是小唾液腺(19例[12.7%]:良性8例,恶性11例[57.9%])。良性腺体实体包括多形性腺瘤(88/105;83.7%),嗜瘤细胞瘤(5/105;4.8%),肌上皮瘤(4/105;3.8%), whatin肿瘤(3/105;2.9%),基底细胞腺瘤(3/105;2.9%),唾液脂肪瘤(2/105;1.9%)。恶性腺体实体包括腺样囊性癌(12;26.7%),黏液表皮样癌(10;22.2%),腺泡细胞癌(6;13.3%),低分化癌(4%;8.9%),腺癌NOS(未另行说明)(4;8.9%),基底细胞腺癌(3;6.7%),癌前多形性腺瘤(3;6.7%),多形性腺癌(2;4.4%),唾液管癌(1;2.2%),上皮-肌上皮癌(2.2%)。结论:sgt具有多种流行病学和组织病理学特征,在腮腺和颌下腺中发病率高,在次要腺体中发病率低,男性居多。
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引用次数: 0
A Correlation of Serum Histamine and Mast Cell Count with the Established Prognosticators in Oral Cancer. 血清组胺和肥大细胞计数与口腔癌预后指标的相关性研究。
Q3 Medicine Pub Date : 2023-04-01 DOI: 10.4103/jmau.jmau_138_20
Nabeel Rashid Hasan, Priyadarshini Dehuri, Amitabh Jena, Srikanta Kanungo

Context: We undertook this study in view of the rising interest in the interactions between tumor cells and mast cells in the tumor microenvironment and the mounting evidence of the role of mast cells in the prognosis of various cancers.

Aims: The aim of the study is to determine the prognostic utility of mast cells and their mediators in oral squamous cell carcinoma (OSCC).

Settings and design: This is a cross-sectional analytical study. A total of 41 cases were studied for determining the correlation of mast cell counts with histopathological prognostic variables and 29 cases for determining the serum histamine levels and to further correlate the values with the histopathologic determinants.

Subjects and methods: Mast cell assessment was done using Toluidine blue stain on tissue sections while histopathological prognostic factors were evaluated with routine stains. The serum histamine levels were obtained by sandwich enzyme linked immunosorbent assay (ELISA).

Statistical analysis: Mann-Whitney U-test was used to test the difference between parameters and a Spearman correlation coefficient was used. P < 0.05 was considered significant for statistical analysis.

Results: We found statistically significant correlations between increased median mast cell counts and higher grade of tumor, presence of lymphovascular invasion, greater depth of invasion, and presence of regional lymph node involvement. The serum histamine values correlated only with the depth of tumor invasion.

Conclusions: Hence, as per our results, we suggest the use of mast cell counts in OSCC cases as a prognostic indicator in conjunction with other clinicopathological factors. At the same time, we acknowledge the fact that extensive studies are required to establish the role of mast cell mediators in the prognostic curve of oral cancer patients.

背景:鉴于人们对肿瘤细胞和肥大细胞在肿瘤微环境中的相互作用越来越感兴趣,以及越来越多的证据表明肥大细胞在各种癌症预后中的作用,我们开展了这项研究。目的:本研究的目的是确定肥大细胞及其介质在口腔鳞状细胞癌(OSCC)中的预后作用。环境和设计:这是一项横断面分析研究。共有41例研究了肥大细胞计数与组织病理学预后变量的相关性,29例研究了血清组胺水平,并进一步将其与组织病理学决定因素相关联。对象和方法:采用甲苯胺蓝染色对组织切片进行肥大细胞评估,常规染色评估组织病理学预后因素。采用夹心酶联免疫吸附试验(ELISA)测定血清组胺水平。统计分析:采用Mann-Whitney u检验检验参数间的差异,采用Spearman相关系数。P < 0.05为差异有统计学意义。结果:我们发现中位肥大细胞计数增加与肿瘤分级高、淋巴血管浸润、浸润深度大、区域淋巴结受累之间存在统计学上的显著相关性。血清组胺值仅与肿瘤浸润深度相关。结论:因此,根据我们的研究结果,我们建议在OSCC病例中使用肥大细胞计数作为预后指标,并结合其他临床病理因素。同时,我们认识到需要进行大量的研究来确定肥大细胞介质在口腔癌患者预后曲线中的作用。
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引用次数: 1
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Journal of Microscopy and Ultrastructure
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