Journal of Neuroscience Methods最新文献_第2页

Neuroimaging of reality: A new approach for investigating neural bases of decision-making with real-world objects 现实的神经成像：研究现实世界物体决策的神经基础的新方法

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2026-01-20 DOI: 10.1016/j.jneumeth.2026.110692

Damien Gabriel , Guillaume Bertrand , Magali Nicolier , Julie Giustiniani

Background

Neuroimaging studies often use computerized tasks, but reliance on virtual stimuli limits ecological validity. Incorporating real object interaction under controlled recording conditions may enhance the study of decision-making processes.

New method

We developed Lab-Life, a device enabling manipulation of real objects while ensuring precise monitoring and compatibility with electrophysiological recordings. Forty-four right-handed healthy volunteers performed two decision-making tasks: the Iowa Gambling Task (IGT, real vs. virtual cards) and the Game of Dice Task (GDT, real vs. virtual dice). Twenty-two participants (11 per task) used Lab-Life (hybrid condition), while additional virtual task groups were included to illustrate typical behavioral and EEG signatures. Object identity and values were tracked with infrared cameras, and EEG was recorded to analyze event-related potentials (ERPs) to outcomes.

Results

Behavioral analyses showed perfect concordance between expected and detected object values in hybrid condition, validating Lab-Life’s automated object recognition. EEG analyses revealed comparable numbers of valid trials and similar ERP patterns between hybrid and virtual task conditions, indicating that the device does not introduce movement artifacts. Participants consistently reported higher enjoyment when manipulating real compared to virtual objects.

Comparison with existing methods

Unlike conventional paradigms relying solely on virtual stimuli, Lab-Life integrates real objects without compromising behavioral or electrophysiological data quality. The device allows precise temporal synchronization between object manipulation and EEG recordings while preserving experimental control.

Conclusions

Lab-Life is a validated methodological tool for combining behavioral and electrophysiological measures with real object manipulation. It offers a flexible and adaptable platform for decision-making, memory, or perceptual tasks, thereby bridging the gap between laboratory experiments and real-life conditions. Larger studies are warranted to further explore its impact on cognitive performance.

神经成像研究通常使用计算机化任务，但依赖虚拟刺激限制了生态有效性。在受控的记录条件下结合真实物体的相互作用可以加强对决策过程的研究。我们开发了Lab-Life，一种能够操纵真实物体的设备，同时确保精确监测和与电生理记录的兼容性。44名健康的右利手志愿者执行了两项决策任务：爱荷华赌博任务（IGT，真实纸牌vs虚拟纸牌）和骰子游戏任务（GDT，真实骰子vs虚拟骰子）。22名参与者（每个任务11人）使用Lab-Life（混合条件），同时包括额外的虚拟任务组来说明典型的行为和脑电图特征。用红外摄像机跟踪目标识别和值，并记录脑电图以分析事件相关电位（ERPs）与结果。结果在混合条件下，行为分析结果显示预期目标值与检测目标值完全一致，验证了Lab-Life的自动目标识别。脑电图分析显示，在混合任务和虚拟任务条件下，有效试验的数量和相似的ERP模式相当，表明该设备不会引入运动伪影。参与者一致表示，与操纵虚拟物体相比，操纵真实物体更有乐趣。与仅依赖虚拟刺激的现有方法相比，Lab-Life集成了真实对象，而不影响行为或电生理数据的质量。该设备允许对象操作和脑电图记录之间的精确时间同步，同时保持实验控制。结论lab - life是一种将行为和电生理测量与实物操作相结合的行之有效的方法工具。它为决策、记忆或感知任务提供了一个灵活和适应性强的平台，从而弥合了实验室实验和现实生活条件之间的差距。需要更大规模的研究来进一步探索它对认知表现的影响。

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引用次数: 0

Corrigendum to “Proper reference selection and re-referencing to mitigate bias in single pulse electrical stimulation data” [J. Neurosci. Methods. 419, (2025) 110461] “适当的参考文献选择和重新参考以减轻单脉冲电刺激数据的偏差”的勘误表[J]。>。[方法]. 419,(2025)110461]。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2026-01-20 DOI: 10.1016/j.jneumeth.2026.110690

Harvey Huang , Joshua A. Adkinson , Michael A. Jensen , Mohammed Hasen , Isabel A. Danstrom , Kelly R. Bijanki , Nicholas M. Gregg , Kai J. Miller , Sameer A. Sheth , Dora Hermes , Eleonora Bartoli

引用次数: 0

Longitudinal multimodal assessment of neuropathy in a porcine neuritis model 猪神经炎模型神经病变的纵向多模态评估。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2026-01-18 DOI: 10.1016/j.jneumeth.2026.110694

Ethan D. Griswold , Matthew S. Zabriskie , Lexie Scarpa , Eric Goold , Henrik Odeen , Lubdha M. Shah , Viola Rieke

Background

Evaluation of animal models is critical in pre-clinical therapeutic research to determine translation potential. Due to their anatomic and physiological resemblance to humans, porcine models are a critical step prior to human translation. Our work evaluates long-term anatomic and behavioral effects (n = 9) over 8 weeks post-neuritis-induction surgery of the common peroneal nerve (CPN).

New method

This model utilizes longitudinal MRI and histological analysis of nerve and muscle, in addition to the use of the algometer and feather test as quantitative sensory testing methods.

Results

Neuritis animals showed heightened rear leg sensitivity on the neuritis side, with significant reduction in maximum tolerated pressure in the majority of dermatomes and significant heightened sensitivity to light touch stimuli. Significant muscle loss was well defined with short- and long-term assessments. MR imaging showed increased signal intensity in CPN-innervated muscles alongside reduction in volume and hypotonia. Histology supports these conclusions as evidenced by the presence of atrophied fibers, fibrosis, and fatty infiltrations. At necropsy, there was an average of 50 % reduction in CPN-innervated muscle weights compared to control leg muscles. Pigs experienced postural and ambulatory abnormalities without inhibiting regular activity.

Comparison with existing method(s)

We expand on established protocols for assessing neuropathic pain (Castel et al., 2016; Hellman et al., 2021b), which assess short-term effects (10 days – 4 weeks) of a peripheral nerve injury.

Conclusion

We have demonstrated and evaluated a longitudinal chronic neuropathic pain porcine model. Variables including medication regime and anatomic sequelae need to be considered as they may affect the quantitative sensory testing and time course of testing.

背景：动物模型的评估在临床前治疗研究中至关重要，以确定翻译潜力。由于它们在解剖和生理上与人类相似，猪模型是人类翻译之前的关键步骤。我们的研究评估了腓总神经（CPN）神经炎诱导手术后8周的长期解剖学和行为学影响（n=9）。新方法：该模型利用纵向MRI和神经和肌肉的组织学分析，除了使用algometer和羽毛测试作为定量的感觉测试方法。结果：神经炎动物表现出神经炎一侧的后腿敏感性增高，大多数皮节的最大耐受压力显著降低，对轻触刺激的敏感性显著增高。通过短期和长期评估明确定义了显著的肌肉损失。磁共振成像显示cpn神经支配肌肉的信号强度增加，同时体积减小和张力降低。组织学支持这些结论，因为纤维萎缩、纤维化和脂肪浸润的存在。尸检发现，与对照组腿部肌肉相比，cpn神经支配的肌肉重量平均减少50%。猪在没有抑制正常活动的情况下出现姿势和运动异常。与现有方法的比较：我们扩展了评估神经性疼痛的既定方案（Castel等人，2016；Hellman等人，2021b），评估周围神经损伤的短期影响（10天- 4周）。结论：我们已经证明并评估了纵向慢性神经性疼痛猪模型。需要考虑包括用药方案和解剖后遗症在内的变量，因为它们可能影响定量感觉测试和测试的时间过程。

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引用次数: 0

Enhancing brain–computer interface performance through source-level attention mechanism: An EEG motor imagery study 通过源级注意机制增强脑机接口性能：一项脑电图运动图像研究。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2026-01-16 DOI: 10.1016/j.jneumeth.2025.110666

Jia-He Lim, Po-Chih Kuo

Background:

Brain–computer interfaces (BCIs) enable direct communication between humans and machines by translating brain signals into control commands. Electroencephalography (EEG) is a commonly used modality in BCI systems due to its non-invasiveness and high temporal resolution. However, EEG-based BCIs often suffer from low signal-to-noise ratios and limited spatial resolution, primarily due to the small number of recording electrodes. Although source estimation techniques can improve spatial specificity, they typically require subject-specific information such as individual brain anatomy or electrode positions, which may not always be available. This study aims to address these challenges by proposing a framework that enhances task-relevant EEG signals using an attention-guided source estimation approach based on coarse predefined brain regions.

New method:

We developed an attention-guided neural network that estimates source-level activity most relevant to the BCI task, without requiring subject-specific structural data. The model uses predefined regions of interest to guide attention mechanisms toward informative spatial features.

Results:

The framework was validated using publicly available motor imagery EEG datasets, achieving strong performance. Comparison with existing methods: Comparative analyses were conducted against baseline models using traditional EEG signals and standard feature extraction methods. This study presents an effective approach for improving EEG-based BCI performance by integrating an attention-guided source estimation network into the decoding pipeline. The method does not rely on subject-specific anatomical information, making it broadly applicable.

Conclusion:

By emphasizing task-relevant source activity, the framework enhances signal quality and classification accuracy, thereby advancing the potential of BCIs for precise and practical applications.

背景：脑机接口（bci）通过将大脑信号转换为控制命令来实现人与机器之间的直接通信。脑电图（EEG）由于其非侵入性和高时间分辨率，是脑机接口系统中常用的一种模式。然而，基于脑电图的脑机接口通常存在低信噪比和有限空间分辨率的问题，主要是由于记录电极数量少。虽然源估计技术可以提高空间特异性，但它们通常需要特定主题的信息，如个体大脑解剖结构或电极位置，这些信息可能并不总是可用的。本研究旨在通过提出一个框架来解决这些挑战，该框架使用基于粗糙预定义脑区域的注意引导源估计方法来增强任务相关的EEG信号。新方法：我们开发了一个注意力引导的神经网络，它可以估计与脑机接口任务最相关的源级活动，而不需要受试者特定的结构数据。该模型使用预定义的兴趣区域来引导注意力机制转向信息空间特征。结果：该框架使用公开可用的运动图像EEG数据集进行了验证，取得了较好的性能。与现有方法的比较：采用传统脑电信号和标准特征提取方法与基线模型进行对比分析。本研究提出了一种有效的方法，通过将注意力引导的源估计网络集成到解码管道中来提高基于脑电图的脑机接口性能。该方法不依赖于特定主题的解剖信息，使其广泛适用。结论：通过强调与任务相关的源活动，该框架提高了信号质量和分类精度，从而提高了脑机接口在精确和实际应用方面的潜力。

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引用次数: 0

Research progress in vivo, in vitro, and in silico models of intracranial atherosclerotic stenosis 颅内动脉粥样硬化性狭窄的体内、体外和计算机模型研究进展。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2026-01-16 DOI: 10.1016/j.jneumeth.2026.110685

Fanqiang Meng , Hanlu Xiang , Mengjie Huo , Jiaqi Wu , Wentao Yao , Hebo Wang

Intracranial atherosclerotic stenosis (ICAS) is a major global cause of stroke with high recurrence risk. However, research on its pathophysiology is limited by difficulties in obtaining histopathological samples and the absence of adequate experimental models. The rising incidence of ICAS imposes a significant burden on families and healthcare systems, highlighting the urgent need for affordable, robust, and reliable animal models to study its prevention and treatment. To better understand the disease, researchers frequently employ animal models to replicate the progression of ICAS in humans. In recent years, many researchers have provided a variety of solutions for modeling methods and intervention protocols in animal models of ICAS, which has greatly improved the success rate of atherosclerosis models. Nevertheless, variations in intervention factors, material selection, and treatment methods have resulted in no consensus on the optimal animal model for ICAS. This review evaluates the strengths and limitations of existing in vivo、in vitro model and computational models of ICAS and the atherosclerotic process, aiming to inform better future models and drive novel stroke-reducing treatment strategies.

颅内动脉粥样硬化性狭窄（ICAS）是全球卒中的主要原因，具有高复发风险。然而，其病理生理学的研究受到组织病理样本获取困难和缺乏足够的实验模型的限制。ICAS发病率的上升给家庭和卫生保健系统带来了沉重的负担，因此迫切需要可负担得起的、健壮的和可靠的动物模型来研究其预防和治疗。为了更好地了解这种疾病，研究人员经常使用动物模型在人类身上复制ICAS的进展。近年来，许多研究者为ICAS动物模型的建模方法和干预方案提供了多种解决方案，大大提高了动脉粥样硬化模型的成功率。然而，干预因素、材料选择和治疗方法的变化导致对ICAS的最佳动物模型没有达成共识。本综述评估了现有的ICAS和动脉粥样硬化过程的体内、体外模型和计算模型的优势和局限性，旨在为未来更好的模型提供信息，并推动新的减少中风的治疗策略。

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引用次数: 0

Micromap: A low-cost multi-channel electrophysiology acquisition system Micromap：一个低成本的多通道电生理采集系统。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2026-01-16 DOI: 10.1016/j.jneumeth.2026.110691

João Pedro Carvalho-Moreira , Luiz Felipe Nogueira Varandas , Matheus Costa Passos , Márcio Flávio Dutra Moraes

Objective

To develop an accessible, low-cost, and open-source system for multi-channel electrophysiological recordings across multiple brain regions, facilitating the investigation of neural dynamics and information flow in long-range integration. Approach: We designed a digital signal acquisition system that integrates two programmable analog-to-digital converter chipsets -- Intan RHD and Texas Instruments ADS -- with an Arduino-based microcontroller. In addition, we introduce a custom-designed headstage and a methodology for spatially distributed electrode placement using a perforated printed circuit board, which interfaces directly with the system to enable local field potential recordings from multiple brain regions. The system successfully recorded biopotentials without significant sample loss and performed comparably to gold-standard systems, enabling recordings across 32 channels at a 2 kHz sampling frequency. We verified signal integrity and stability under both experimental and simulated conditions, confirming the platform’s suitability for electrophysiology. Main results: We present a comprehensive, low-cost bioengineering solution for distributed electrophysiological recordings, enabling detailed investigation of brain dynamics through synchronized neural activity across regions. Significance: This open-source platform enables neuroscience laboratories to investigate large-scale neural interactions with high temporal precision, expanding access to systems neuroscience tools essential for understanding interregional brain connectivity.

目的：开发一种可访问的、低成本的、开源的多通道脑电生理记录系统，为研究远程整合中的神经动力学和信息流提供便利。方法：我们设计了一个数字信号采集系统，该系统集成了两个可编程模数转换器芯片组（Intan RHD和Texas Instruments ADS）和基于arduino的微控制器。此外，我们还介绍了一个定制设计的头台和一种使用穿孔印刷电路板进行空间分布电极放置的方法，该方法直接与系统接口，以实现来自多个大脑区域的局部场电位记录。该系统成功地记录了生物电位，没有明显的样品损失，与金标准系统相比，能够以2kHz的采样频率记录32个通道。我们在实验和模拟条件下验证了信号的完整性和稳定性，证实了该平台适合电生理学。我们提出了一种全面的、低成本的生物工程解决方案，用于分布式电生理记录，通过跨区域的同步神经活动来详细研究大脑动力学。意义：这个开源平台使神经科学实验室能够以高时间精度研究大规模的神经相互作用，扩大了对理解区域间大脑连接所必需的系统神经科学工具的访问。

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引用次数: 0

Longitudinal evaluation of white matter tracts post traumatic brain injury using a pediatric porcine model 使用儿童猪模型对创伤性脑损伤后白质束的纵向评价

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2026-01-13 DOI: 10.1016/j.jneumeth.2026.110684

Morgan H. LaBalle , Wenwu Sun , Ishfaque Ahmed , William D. Reeves , Moira F. Taber , Sydney E. Sneed , Erin E. Kaiser , Franklin D. West , Qun Zhao

Background

Traumatic brain injury (TBI) induced white matter damage in the brain has a lasting impact on brain functions, leading to cognitive and motor impairments.

New method

To better predict patient deficits, develop treatment plans, and identify novel therapies, longitudinal effects of TBI on white matter tracts were evaluated using a pediatric porcine model. Diffusion-weighted MRI data from a cohort of female piglets (n = 22) consisting of three groups, including sham, mild- and severe-TBI pigs, was acquired at 4 time points (pre-TBI, 1D-, 63D-, and 119D-post TBI). A group of common white matter tracts was generated using a probabilistic independent component tractography approach. The tracts were evaluated, both laterally between two hemispheres and longitudinally among 4 time points, to measure changes in injured tracts over a 120-day period. Fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD), and axial diffusivity (AD) were calculated for these tracts and compared.

Results

9 pairs of white matter tracts were generated, 7 of which corresponded to those found in adult pigs in the Porcine Neurological Imaging Space. Our results showed consistently lower FA and higher diffusivity in the ipsilateral hemisphere (with TBI injury) for all 9 pairs. Longitudinal changes in these metrics indicated a complex interplay between typical development and TBI recovery.

Comparison with existing methods

This method captured similar trends in FA and diffusivity as found in empirical data.

Conclusion

The probabilistic IC tractography approach effectively characterized lateral and longitudinal changes to white matter tracts using a multi-group and multi-time-point pediatric porcine TBI model.

背景创伤性脑损伤（TBI）引起的脑白质损伤对脑功能有持久的影响，导致认知和运动障碍。新方法为了更好地预测患者的缺陷，制定治疗计划，并确定新的治疗方法，使用儿科猪模型评估脑外伤对白质束的纵向影响。在4个时间点（TBI前、1D、63D和119d后）获得了一组母仔猪（n = 22）的弥散加权MRI数据。该数据由三组仔猪组成，包括假性、轻度和重度TBI猪。一组普通白质束是用概率独立分量的方法生成的。在120天的时间内，对两个半球之间的横向和4个时间点之间的损伤束进行评估，以测量损伤束的变化。计算各向异性分数（FA）、平均扩散系数（MD）、径向扩散系数（RD）和轴向扩散系数（AD）并进行比较。结果共生成9对白质束，其中7对与猪神经成像空间中成年猪的白质束相对应。我们的研究结果显示，所有9对患者的同侧半球（TBI损伤）FA均较低，弥散性较高。这些指标的纵向变化表明典型发育与TBI恢复之间存在复杂的相互作用。与现有方法的比较该方法捕获了与经验数据中发现的FA和扩散率相似的趋势。结论采用多组、多时间点的小儿猪脑外伤模型，概率IC通道造影方法可有效表征脑白质束的横向和纵向变化。

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引用次数: 0

Action tremor kinematics exhibit features of stabilization dysfunction in essential tremor patients 特发性震颤患者的行动性震颤运动学表现出稳定功能障碍的特征。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2026-01-13 DOI: 10.1016/j.jneumeth.2026.110680

Madelyn Mendlen , Rex N. Tien , Keanu Chee , Jonathan P. Platt , Jannika Machnik , Eric Bayman , Chiagoziem Anigbogu , Drew Kern , Steven G. Ojemann , John A. Thompson , Daniel R. Kramer

Background

Action tremor that begins with voluntary movement is a common manifestation of essential tremor (ET). We investigated a novel approach to detect reach direction and tremor direction across naturalistic reaches, elucidating this relationship.

Methods

ET patients undergoing awake DBS implantation surgery performed a center-out reaching task, which elicited multi-joint, naturalistic reaching movements made to evenly spaced radial targets. This project utilized a deep-learning based markerless motion tracking system to monitor movements in 3-dimensions. For analysis, to test novel hypotheses, we compared the direction of the tremor vectors (during hold) to the direction of the reach vector, as well as to the other tremor direction vectors for each subject.

Results

We analyzed 13 limb recordings from 9 subjects. Tremor directions were not significantly dependent on preceding reach directions in any recording (p > 0.05). The distribution of observed tremor directions was significantly different from the expected distribution of random vectors for all 11 analyzed recordings (likelihood of observed tremor directions vs. randomly generated vectors, p < 0.01).

Comparison with existing methods

Our markerless capture method for tremor measurement does not rely on accelerometry or electromyography and does not require fiducials for tracking and combines two open-source python toolkits to leverage computer vision for three-dimensional pose estimation. Further we test a novel solution for measuring the relationship between tremor direction and movement.

Conclusions

Here, we describe a novel method of tremor detection. Our findings suggest that an action tremor is not generated as an oscillation of a function involved in reach, such as braking, but is consistent with a function involved in stabilization to maintain a posture.

背景：以自主运动开始的行动性震颤是特发性震颤（ET）的常见表现。我们研究了一种新的方法来检测到达方向和地震方向跨越自然到达，阐明了这种关系。方法：接受清醒DBS植入手术的ET患者执行中心向外伸展任务，该任务引发多关节自然地向均匀间隔的径向目标伸展运动。该项目利用基于深度学习的无标记运动跟踪系统来监测三维运动。为了进行分析，为了测试新的假设，我们将震颤矢量的方向（在保持期间）与到达矢量的方向以及每个受试者的其他震颤方向矢量进行了比较。结果：我们分析了9名受试者的13条肢体记录。在任何记录中，震颤方向与先前到达方向无显著相关性（p > 0.05）。在所有11个分析记录中，观察到的震颤方向分布与随机矢量的预期分布有显著差异（观察到的震颤方向的可能性与随机生成的矢量，p < 0.01）。与现有方法的比较：我们的无标记捕获震颤测量方法不依赖于加速度计或肌电图，也不需要跟踪基准，并结合两个开源python工具包来利用计算机视觉进行三维姿态估计。进一步，我们测试了一种新的解决方案来测量震颤方向和运动之间的关系。结论：在这里，我们描述了一种新的震颤检测方法。我们的研究结果表明，行动性震颤不是作为与到达有关的功能（如制动）的振荡产生的，而是与与保持姿势稳定有关的功能一致。

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引用次数: 0

Custom filaments for intraluminal artery occlusion models of stroke in mice 用于小鼠脑卒中腔内动脉闭塞模型的定制细丝

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2026-01-12 DOI: 10.1016/j.jneumeth.2026.110686

Renata Veloso de Souza, Nayara Mary da Silva Alves, Julia Chiacchio Vianna, Thamires Ignacio de Lima, Bruna Rodrigues Coelho, Carlos Henrique Barbosa dos Santos, Newton G. Castro, Jorge Luiz Mendonça Tributino

Background

Available treatments for ischemic stroke have severe limitations and innovation is hampered by a major bench-to-bedside translational block, demanding refinement of animal models. Intraluminal filament occlusion of the middle cerebral artery (MCA) in rodents is a standard stroke model, requiring atraumatic and effective occluding filaments to fit each animal.

New method

We describe a simple and low-cost method to manufacture silicone-coated arterial occluder filaments, with accessible materials. This technique yields cylindrical silicone coatings of predictable and reproducible length and diameter, which can be adapted for different species, sizes of animals and blood vessels.

Results

200 filaments produced with a 6–0 mononylon suture and mold-coated silicone tips were analyzed. Using PE-10 polyethylene tubing stretched to various inner diameters as molds, the silicone tips were nearly cylindrical, with average diameters ranging from 0.22 to 0.28 mm. The diameters measured along each silicone tip had a coefficient of variation of only 4.0 %. Laser Doppler showed stable blood flow reduction in the MCA territory of Swiss mice, with only 2.4 % change of flow during a 30 min occlusion. Motor deficits and corticostriatal infarcts were as expected for similar MCA stroke models.

Comparison with existing methods: Unlike others, our molding procedure yielded filaments with a continuous range of diameters, which could be made to fit each mouse’s arteries and adjusted for other species.

Conclusion

Having an assortment of custom filaments may improve the success rate and reproducibility of transient MCA occlusion, aiding in the discovery of new potential treatments for stroke.

背景：缺血性中风的现有治疗方法有严重的局限性，创新受到主要的从实验室到床边的转化障碍的阻碍，需要改进动物模型。鼠类大脑中动脉腔内丝闭塞是一种标准的脑卒中模型，需要无伤性和有效的闭塞丝来适应每只动物。新方法我们描述了一种简单、低成本、材料易于获取的制备硅涂层动脉闭塞细丝的方法。这种技术产生的圆柱形硅树脂涂层具有可预测和可复制的长度和直径，可以适应不同的物种、大小的动物和血管。结果对用6-0尼龙缝合和模具包覆硅胶针尖生产的200根细丝进行了分析。使用PE-10聚乙烯管拉伸到不同的内径作为模具，硅胶尖端接近圆柱形，平均直径为0.22 ~ 0.28 mm。沿着每个硅胶尖端测量的直径变化系数仅为4.0 %。激光多普勒显示瑞士小鼠MCA区域的血流稳定减少，在30 min的闭塞期间，血流变化仅为2.4 %。运动缺陷和皮质纹状体梗死在类似的MCA卒中模型中是预期的。与现有方法的比较：与其他方法不同的是，我们的成型过程产生的细丝具有连续的直径范围，可以适应每只老鼠的动脉，并根据其他物种进行调整。结论定制不同种类的纤维可以提高短暂性中动脉闭塞的成功率和可重复性，有助于发现新的潜在的脑卒中治疗方法。

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引用次数: 0

Interactions between external and internal attention processes during working memory 工作记忆过程中外部和内部注意过程的相互作用

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2026-01-12 DOI: 10.1016/j.jneumeth.2026.110687

András Puszta , Rene Huster , Anne-Kristin Solbakk , Ingrid Funderud , Tor Endestad , Venke Arntsberg Grane

Background

The interaction between internally and externally driven attention (IDA and EDA) during visual working memory tasks has been primarily investigated separately. There is growing evidence that EDA and IDA interact closely during working memory. However, it seems that previous studies, especially those utilizing EEG, did not modulate EDA and IDA within the same task. Thus, there is a paucity of information on how the two processes interact with each other on the neural level.

New method

We developed a novel paradigm, the PUMA Task, to investigate the interplay between IDA and EDA. This task allows for simultaneous manipulation of IDA and EDA by varying the number of items to be remembered and the complexity of the visual stimuli.

Results

EEG power spectra, eye-tracking measures (pupillometry and fixation disparity) and behavioral response times were sensitive to the interaction between IDA and EDA. Alpha-beta cross-frequency coupling was primarily influenced by EDA.

Comparison with existing methods

Previous studies often modulated either EDA or IDA, but not both within the same task. The PUMA task enables concurrent manipulation of both attentional processes, providing a more comprehensive understanding of their interaction.

Conclusions

The PUMA task is a valuable tool for investigating the interplay between IDA and EDA during visual working memory. Our findings suggest that IDA and EDA are distinct attentional processes that dynamically interact, affecting working memory performance and neural correlates. Importantly, our newly developed task can be employed to more selectively assess attentional subprocesses in neuropsychiatric and neurological disorders.

在视觉工作记忆任务中，内驱动和外驱动注意（IDA和EDA）之间的相互作用主要是单独研究的。越来越多的证据表明，EDA和IDA在工作记忆过程中密切相互作用。然而，以往的研究，特别是利用脑电图的研究，似乎并没有在同一任务中调节EDA和IDA。因此，关于这两个过程如何在神经层面上相互作用的信息缺乏。我们开发了一个新的范式，PUMA任务，来研究IDA和EDA之间的相互作用。该任务允许通过改变要记住的项目数量和视觉刺激的复杂性来同时操作IDA和EDA。结果seeg功率谱、眼球追踪测量（瞳孔测量和注视视差）和行为反应时间对IDA和EDA的交互作用较为敏感。α - β交叉频率耦合主要受EDA的影响。与现有方法的比较以往的研究通常会调节EDA或IDA，但不会在同一任务中同时调节EDA或IDA。PUMA任务可以同时操作两个注意过程，从而更全面地了解它们之间的相互作用。结论PUMA任务是研究视觉工作记忆中IDA和EDA相互作用的重要工具。我们的研究结果表明，IDA和EDA是不同的注意过程，它们动态地相互作用，影响工作记忆的表现和神经相关。重要的是，我们新开发的任务可以用于更有选择性地评估神经精神和神经疾病的注意亚过程。

{"title":"Interactions between external and internal attention processes during working memory","authors":"András Puszta , Rene Huster , Anne-Kristin Solbakk , Ingrid Funderud , Tor Endestad , Venke Arntsberg Grane","doi":"10.1016/j.jneumeth.2026.110687","DOIUrl":"10.1016/j.jneumeth.2026.110687","url":null,"abstract":"<div><h3>Background</h3><div>The interaction between internally and externally driven attention (IDA and EDA) during visual working memory tasks has been primarily investigated separately. There is growing evidence that EDA and IDA interact closely during working memory. However, it seems that previous studies, especially those utilizing EEG, did not modulate EDA and IDA within the same task. Thus, there is a paucity of information on how the two processes interact with each other on the neural level.</div></div><div><h3>New method</h3><div>We developed a novel paradigm, the PUMA Task, to investigate the interplay between IDA and EDA. This task allows for simultaneous manipulation of IDA and EDA by varying the number of items to be remembered and the complexity of the visual stimuli.</div></div><div><h3>Results</h3><div>EEG power spectra, eye-tracking measures (pupillometry and fixation disparity) and behavioral response times were sensitive to the interaction between IDA and EDA. Alpha-beta cross-frequency coupling was primarily influenced by EDA.</div></div><div><h3>Comparison with existing methods</h3><div>Previous studies often modulated either EDA or IDA, but not both within the same task. The PUMA task enables concurrent manipulation of both attentional processes, providing a more comprehensive understanding of their interaction.</div></div><div><h3>Conclusions</h3><div>The PUMA task is a valuable tool for investigating the interplay between IDA and EDA during visual working memory. Our findings suggest that IDA and EDA are distinct attentional processes that dynamically interact, affecting working memory performance and neural correlates. Importantly, our newly developed task can be employed to more selectively assess attentional subprocesses in neuropsychiatric and neurological disorders.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"428 ","pages":"Article 110687"},"PeriodicalIF":2.3,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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