Journal of Neuroscience Methods最新文献_第6页

Outcome processing response coupled to feedback-related EEG dynamics during discrete and continuous performance monitoring 在离散和连续性能监测中，结果处理响应与反馈相关的脑电动态耦合。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2025-11-19 DOI: 10.1016/j.jneumeth.2025.110629

Ruijie Luo , Jianjun Meng , Yuxuan Wei , Ximing Mai , Guangye Li

Background

Error-related potential (ErrP) reflects the inconsistency between internal expectation and external feedback outcome. Despite the exploration of numerous experimental paradigms, ErrP components exhibit distinct latency and amplitude across different paradigms. However, previous studies have not quantitatively correlated potential influencing factors with this ErrP variability. Additionally, these qualitatively analyzed factors offer limited predictions for ErrP in new paradigms.

New method

We proposed that a neutral condition removing goal-directed outcome expectations reflects cross-paradigm variability in correct and erroneous outcome responses. This neutral condition was designed as a control condition for each paradigm. Three different paradigms were designed to provide discrete and continuous varied feedback outcomes. Correlations were assessed between neutral condition responses and correct and erroneous outcome responses in latency and amplitude. The predictive effectiveness of neutral condition responses for new paradigms was further evaluated through single-trial cross-paradigm classification.

Results

Correct and erroneous outcome responses were observed to have significant latency and amplitude coupling with these neutral condition responses in the middle frontal and bilateral parietal regions. Results from source reconstruction, pupillometry data, and workload score confirm that the neutral condition serves as the baseline response for outcome processing responses. This baseline relationship explains the cross-paradigm ErrP variability.

Comparison with existing methods

The single-trial decoding results show that utilizing neutral condition responses can significantly increase the accuracy of cross-paradigm classification by up to 7 % and 17 % with covariance-based and amplitude-based approaches.

Conclusion

These findings provide a quantitative physiological explanation for cross-paradigm ErrP variability and promote transfer learning applications in ErrP-based BCIs.

背景：错误相关电位（ErrP）反映了内部期望与外部反馈结果之间的不一致性。尽管探索了许多实验范式，但ErrP成分在不同范式中表现出不同的潜伏期和振幅。然而，以往的研究并没有定量地将潜在的影响因素与这种ErrP变异性联系起来。此外，这些定性分析的因素对新范式下的ErrP提供了有限的预测。新方法：我们提出了一个去除目标导向结果预期的中性条件，反映了正确和错误结果反应的跨范式可变性。该中性条件被设计为每个范式的控制条件。设计了三种不同的范例来提供离散和连续的不同反馈结果。评估中性条件反应与正确和错误结果反应在潜伏期和幅度上的相关性。通过单试验跨范式分类进一步评估中性条件反应对新范式的预测效果。结果：正确和错误的结果反应在额叶中部和双侧顶叶区与这些中性条件反应有显著的潜伏期和振幅耦合。来自源重构、瞳孔测量数据和工作量评分的结果证实，中性条件是结果处理反应的基线反应。这种基线关系解释了跨范式ErrP的可变性。与现有方法的比较：单次试验解码结果表明，使用中性条件反应可以显著提高基于协方差和基于幅度的跨范式分类方法的准确率，分别提高7%和17%。结论：这些发现为ErrP跨范式变异提供了定量生理学解释，并促进了迁移学习在基于ErrP的脑机接口中的应用。

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引用次数: 0

Uncertainty in population receptive field estimates revealed by variational qPRF 变分qPRF揭示的群体接受野估计的不确定性

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2025-11-19 DOI: 10.1016/j.jneumeth.2025.110628

Sebastian Waz , Yalin Wang , Zhong-Lin Lu

Background:

Population receptive field (pRF) modeling is a cornerstone of retinotopic mapping in visual neuroscience, enabling precise mapping of visual stimulus processing in the human brain. However, pRF estimates are influenced by multiple sources of variability, including scanner properties, neurovascular coupling, physiological noise, and task-related factors. Traditionally, these estimates are treated as definitive because quantifying variance has been computationally infeasible.

New method:

We introduce qPRF-v, an innovative extension of the qPRF software which computes pRF point estimates over 1,000 times faster than existing packages while preserving goodness-of-fit (Waz et al., 2025a).

Results:

Leveraging variational inference, qPRF-v efficiently approximates the posterior variance of pRF model parameters, revealing substantial uncertainty, particularly in parameters governing neural dynamics (e.g., compressive exponent) compared to those defining receptive field centers.

Comparison with existing methods:

To evaluate the impact of variance quantification, we compared population-level retinotopic maps generated via simple averaging (the prior standard) and

R^{2}

weighted averaging with those using inverse-variance weighted averaging. Inverse-variance weighted averaging significantly enhanced map quality, yielding naturally denoised representations with improved eccentricity detail.

Conclusions:

By directly addressing variability, qPRF-v not only increases the accuracy of retinotopic mapping but also enables more robust, individualized analyses of visual processing. This advancement has profound implications for studying visual function in health and disease, paving the way for personalized neuroscience and improved brain atlases for research and clinical applications.

背景：群体感受野（pRF）模型是视觉神经科学中视网膜定位的基础，能够精确地映射人类大脑的视觉刺激处理过程。然而，pRF估计受到多种变异性来源的影响，包括扫描仪特性、神经血管耦合、生理噪声和任务相关因素。传统上，这些估计被视为确定的，因为量化方差在计算上是不可行的。新方法：我们引入了qPRF-v，这是qPRF软件的创新扩展，它计算pRF点估计的速度比现有软件包快1000倍，同时保持拟合优度（Waz等人，2025a）。结果：利用变分推理，qPRF-v有效地近似pRF模型参数的后验方差，揭示了实质性的不确定性，特别是在控制神经动力学的参数（例如，压缩指数）与定义感受野中心的参数相比。与现有方法的比较：为了评估方差量化的影响，我们比较了通过简单平均（先验标准）和R2加权平均生成的人群水平视网膜定位图与使用反方差加权平均生成的人群水平视网膜定位图。逆方差加权平均显著提高了地图质量，产生了带有改进偏心细节的自然去噪表示。结论：通过直接处理变异，qPRF-v不仅提高了视网膜定位的准确性，而且使视觉处理的分析更加稳健、个性化。这一进展对研究健康和疾病中的视觉功能具有深远的意义，为个性化神经科学和改善研究和临床应用的大脑图谱铺平了道路。

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引用次数: 0

3-dimensional morphological analysis of microglia in the sheep brain 绵羊脑小胶质细胞的三维形态学分析。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2025-11-14 DOI: 10.1016/j.jneumeth.2025.110624

Victoria Stock , Emily Hadzajlic , Angela A. Connelly , Jaspreet K. Bassi , Alemayehu H. Jufar , Clive N. May , Yugeesh R. Lankadeva , Song T. Yao , Lindsea C. Booth

Background

Activated microglia change their morphology from small cell bodies with highly ramified branches to become more amoeboid with shorter branches. Accurate morphometric assessment is key to detecting subtle changes in microglia state. Whilst there are many existing methods of analysis, 3-dimensional analysis using Imaris® software is widely used. Despite its widespread use, there is a lack of reporting on what is required from users to ensure accurate and reproducible tracing – including little to no rational for the parameters chosen, and no comparisons between microscope types and magnifications.

New method

We first investigated the possibilities and pitfalls of using Imaris, exploring the effect of microscope type, magnification and different investigators on analysis of microglia from naïve Merino ewes.

Results

Tracings of widefield images were highly variable, whereas tracings of confocal images provided more accurate results. The greatest consistency between analysts was achieved using confocal images taken with a 20x objective; however, the greatest tracing accuracy was achieved using confocal images taken with a 40x objective.

Comparison with existing methods

Having established this, changes in microglia morphology were assessed in two groups of sheep; naïve and a group that underwent cardiopulmonary bypass, a procedure known to induce microglial activation. Three-dimensional microglial analysis allowed detection of more markers of activation than previous 2-dimensional analysis.

Conclusions

This paper describes step-by-step instructions, as well as notes of caution at critical points in the analysis workflow, with the aim of helping those new to microglial analysis avoid pitfalls and improve analysis consistency and accuracy.

背景：被激活的小胶质细胞从具有高度分支的小细胞体转变为具有较短分支的变形虫。准确的形态计量评估是检测小胶质细胞状态细微变化的关键。虽然有许多现有的分析方法，使用Imaris®软件的三维分析被广泛使用。尽管它被广泛使用，但缺乏关于用户需要什么来确保准确和可重复的追踪的报告——包括很少或根本没有合理的参数选择，也没有显微镜类型和放大倍数之间的比较。新方法：我们首先探讨了使用Imaris的可能性和缺陷，探讨了显微镜类型、放大率和不同的研究者对naïve美利奴母羊小胶质细胞分析的影响。结果：宽视场图像的跟踪是高度可变的，而共聚焦图像的跟踪提供更准确的结果。分析人员之间的最大一致性是通过使用20倍物镜拍摄的共聚焦图像实现的；然而，使用40倍物镜拍摄的共聚焦图像达到了最大的跟踪精度。与现有方法的比较：在确定了这一点后，对两组绵羊的小胶质细胞形态学变化进行了评估；Naïve和另一组接受了体外循环，这是一种已知可以诱导小胶质细胞激活的手术。三维小胶质细胞分析可以检测到比以前的二维分析更多的激活标记。结论：本文描述了逐步说明，以及在分析工作流程中的关键点注意事项，旨在帮助那些刚接触小胶质细胞分析的人避免陷阱，提高分析的一致性和准确性。

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引用次数: 0

Do it yourself: Creating 3D brain-surface models and custom-made brain matrices for guided sectioning using photogrammetry and three-dimensional printing technology 自己动手：使用摄影测量和三维打印技术创建3D脑表面模型和定制的脑矩阵，用于引导切片。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2025-11-14 DOI: 10.1016/j.jneumeth.2025.110626

Sophie Holtz , Julio Hechavarria , Mirjam Knörnschild , Constance Scharff

Background

Neuroethologists study many non-model animals. The development of techniques for precise and standardized histological brain analysis is key for understanding neural mechanisms across species.

New method

Here we present a novel cost-effective approach to generate species-specific brain matrices for precise and reproducible trimming, blocking, and sectioning of brain tissue. To produce the matrix, we took high-quality photographs of two male CP brains and then used the open-source 3D graphics suite Blender and its inexpensive photogrammetry plug-in SnapMesh to generate the 3D brain surface model. The brain matrix was then modeled in Blender, and 3D printed.

Results

Using this approach, we produced the first 3D brain surface model and brain matrix for Seba’s short-tailed fruit bat Carollia perspicillata, CP, and assessed its quality using CP brains. Our brain matrix facilitates and standardizes trimming or blocking of brains, providing consistent access to brain regions of CP in the sagittal and coronal planes. This workflow should be suitable for most vertebrate brains.

Comparison with existing methods

Photogrammetry offers a viable, inexpensive alternative to 3D- or CT-scanners. Our workflow is not only cheaper than alternatives, but does not require blocking the brain during preparation of the 3D surface model, resulting in no tissue loss.

Conclusion

The cost-effectiveness and the tissue-preserving features will benefit researchers globally, particularly those with limited financial means and/or few valuable specimens. By providing an accessible, customizable and reproducible workflow, our study represents a significant step toward democratizing advanced neuroscience across diverse species.

背景：神经行为学家研究了许多非模式动物。精确和标准化的脑组织组织学分析技术的发展是理解跨物种神经机制的关键。新方法：在这里，我们提出了一种新的经济有效的方法来生成物种特异性脑基质，用于精确和可重复的脑组织修剪，阻断和切片。为了生成矩阵，我们拍摄了两个男性CP大脑的高质量照片，然后使用开源3D图形套件Blender及其廉价的摄影测量插件SnapMesh生成3D脑表面模型。然后在Blender中建模大脑矩阵，并进行3D打印。结果：利用该方法，我们制作了首个Seba短尾果蝠（Carollia perspicillata， CP）的三维脑表面模型和脑矩阵，并利用CP脑对其质量进行了评估。我们的脑基质促进和规范了大脑的修剪或阻塞，提供了在矢状面和冠状面进入CP脑区域的一致通道。这种工作流程应该适用于大多数脊椎动物的大脑。与现有方法的比较：摄影测量为3D或ct扫描仪提供了一种可行的、廉价的替代方法。我们的工作流程不仅比替代方案便宜，而且在制备3D表面模型时不需要阻塞大脑，因此不会导致组织损失。结论：成本效益和组织保存特性将使全球的研究人员受益，特别是那些资金有限和/或珍贵标本较少的研究人员。通过提供一个可访问的、可定制的和可重复的工作流程，我们的研究代表了在不同物种中实现先进神经科学民主化的重要一步。

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引用次数: 0

Detection of subarachnoid hemorrhage by bilateral transcranial laser doppler fluxmetry allows long-term studies in mice 双侧经颅激光多普勒通量法检测蛛网膜下腔出血允许在小鼠中进行长期研究。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2025-11-14 DOI: 10.1016/j.jneumeth.2025.110625

Biyan Nathanael Harapan , Julian Schwarting , Andrea Cattaneo , Dilara Ozsavas , Uta Mamrak , Gian Marco Calandra , Xiangjiang Lin , Nikolaus Plesnila , Nicole Angela Terpolilli

Background

The murine middle cerebral artery (MCA) perforation model is widely used to study subarachnoid hemorrhage (SAH) but typically requires invasive intracranial pressure (ICP) monitoring to prove successful induction of hemorrhage. However, ICP probe placement causes substantial additional parenchymal damage, confounding long-term structural and functional assessments. Therefore, we investigated whether bilateral transcranial cerebral blood flow (CBF) monitoring, which does not cause any parenchymal damage, can reliably detect SAH induction in mice.

New method

SAH was induced in 25 male C57BL/6 mice with simultaneous ICP and bilateral CBF monitoring over the MCA territory. An additional 40 mice underwent SAH surgery using only bilateral CBF monitoring (without ICP monitoring). Ipsilateral and contralateral CBF and ICP curves were analyzed to identify reliable predictors of successful SAH induction.

Results

MR imaging revealed progressive parenchymal injury caused by the ICP probe. SAH was reproducibly induced without ICP monitoring when bilateral CBF dropped > 50 % from baseline within 90 s after perforation. Using this minimally invasive approach, SAH could be consistently induced by different surgeons, with severity and outcomes comparable to those with ICP monitoring.

Comparison with existing methods and conclusion

ICP probe placement introduces parenchymal damage and may therefore limit the validity of long-term outcome studies. Our findings suggest that bilateral CBF monitoring can effectively replace ICP measurement, reducing invasiveness and enabling valid long-term SAH experiments in mice.

背景：小鼠大脑中动脉（MCA）穿孔模型被广泛用于研究蛛网膜下腔出血（SAH），但通常需要侵入性颅内压（ICP）监测来证明成功诱导出血。然而，ICP探针的放置会导致大量额外的实质损伤，混淆长期的结构和功能评估。因此，我们研究了双侧经颅脑血流（CBF）监测是否可以可靠地检测小鼠SAH诱导，因为CBF不造成任何实质损伤。新方法：25只雄性C57BL/6小鼠，同时进行颅内压监测和双侧颅脑血流监测。另外40只小鼠接受SAH手术，仅使用双侧CBF监测（不使用ICP监测）。分析同侧和对侧CBF和ICP曲线，以确定成功诱导SAH的可靠预测因素。结果：mri显示颅内压探头引起的进行性脑实质损伤。当穿孔后90秒内双侧CBF较基线下降50%时，在没有颅内压监测的情况下可重复诱导SAH。使用这种微创入路，SAH可以由不同的外科医生一致诱导，其严重程度和结果与ICP监测相当。与现有方法的比较和结论：ICP探针置入会导致实质损伤，因此可能限制长期结果研究的有效性。我们的研究结果表明，双侧CBF监测可以有效地取代ICP测量，减少侵入性，并在小鼠中进行有效的长期SAH实验。

{"title":"Detection of subarachnoid hemorrhage by bilateral transcranial laser doppler fluxmetry allows long-term studies in mice","authors":"Biyan Nathanael Harapan , Julian Schwarting , Andrea Cattaneo , Dilara Ozsavas , Uta Mamrak , Gian Marco Calandra , Xiangjiang Lin , Nikolaus Plesnila , Nicole Angela Terpolilli","doi":"10.1016/j.jneumeth.2025.110625","DOIUrl":"10.1016/j.jneumeth.2025.110625","url":null,"abstract":"<div><h3>Background</h3><div>The murine middle cerebral artery (MCA) perforation model is widely used to study subarachnoid hemorrhage (SAH) but typically requires invasive intracranial pressure (ICP) monitoring to prove successful induction of hemorrhage. However, ICP probe placement causes substantial additional parenchymal damage, confounding long-term structural and functional assessments. Therefore, we investigated whether bilateral transcranial cerebral blood flow (CBF) monitoring, which does not cause any parenchymal damage, can reliably detect SAH induction in mice.</div></div><div><h3>New method</h3><div>SAH was induced in 25 male C57BL/6 mice with simultaneous ICP and bilateral CBF monitoring over the MCA territory. An additional 40 mice underwent SAH surgery using only bilateral CBF monitoring (without ICP monitoring). Ipsilateral and contralateral CBF and ICP curves were analyzed to identify reliable predictors of successful SAH induction.</div></div><div><h3>Results</h3><div>MR imaging revealed progressive parenchymal injury caused by the ICP probe. SAH was reproducibly induced without ICP monitoring when bilateral CBF dropped > 50 % from baseline within 90 s after perforation. Using this minimally invasive approach, SAH could be consistently induced by different surgeons, with severity and outcomes comparable to those with ICP monitoring.</div></div><div><h3>Comparison with existing methods and conclusion</h3><div>ICP probe placement introduces parenchymal damage and may therefore limit the validity of long-term outcome studies. Our findings suggest that bilateral CBF monitoring can effectively replace ICP measurement, reducing invasiveness and enabling valid long-term SAH experiments in mice.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"426 ","pages":"Article 110625"},"PeriodicalIF":2.3,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A modified α-synuclein seed amplification assay in Lewy body dementia using Raman spectroscopy and machine learning analysis 利用拉曼光谱和机器学习分析对路易体痴呆患者α-突触核蛋白种子扩增进行检测

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2025-11-12 DOI: 10.1016/j.jneumeth.2025.110617

Nathan P. Coles , Suzan Elsheikh , Alaa Gouda , Agathe Quesnel , Lucy Butler , Ojodomo J. Achadu , Meez Islam , Karunakaran Kalesh , Annalisa Occhipinti , Claudio Angione , Jon Marles-Wright , David J. Koss , Alan J. Thomas , Tiago F. Outeiro , Panagiota S. Filippou , Ahmad A. Khundakar

Background

Lewy body dementias (LBD), comprising dementia with Lewy bodies (DLB) and Parkinson’s disease dementia (PDD), are defined by misfolded α-synuclein aggregation. Seed amplification assays (SAAs), such as RT-QuIC, enable sensitive detection of α-synuclein aggregates but typically provide binary readouts and require fluorescence labeling. Raman spectroscopy offers a label-free approach to detect subtle biochemical changes, and its diagnostic potential can be enhanced with machine learning.

Objectives

This proof-of-concept study aimed to evaluate whether Raman spectroscopy combined with machine learning can improve SAA-based discrimination of LBD from controls in cerebrospinal fluid (CSF).

Methods

We analyzed a small number of post-mortem CSF samples from pathologically confirmed DLB (n = 2), PDD (n = 2), and controls (n = 2) using a 7-day SAA. Raman spectra were collected on Days 1, 4, and 7 and analyzed using principal component analysis (PCA) and uniform manifold approximation and projection (UMAP).

Results

Following SAA, both PCA and UMAP distinguished combined LBD samples from controls within 24 h (Day 1), reflecting biochemical changes consistent with α-synuclein fibrillation. Spectral shifts indicated decreased α-helical content with increased β-sheet structures. No consistent separation between DLB and PDD was observed.

Conclusion

This preliminary study demonstrates that combining Raman spectroscopy with machine learning can enable rapid, label-free detection of disease-specific changes. Despite the very limited sample size, these findings highlight the potential of this novel workflow and strongly warrant its validation in larger cohorts.

背景：路易体痴呆（LBD）包括路易体痴呆（DLB）和帕金森病痴呆（PDD），由α-突触核蛋白错误折叠聚集而定义。种子扩增试验（SAAs），如RT-QuIC，能够灵敏地检测α-突触核蛋白聚集，但通常提供二进制读数并需要荧光标记。拉曼光谱提供了一种无标签的方法来检测细微的生化变化，它的诊断潜力可以通过机器学习来增强。目的：本概念验证研究旨在评估拉曼光谱结合机器学习是否可以改善脑脊液（CSF）中基于saa的LBD与对照组的区分。方法采用7天SAA对病理证实的DLB （n = 2）、PDD （n = 2）和对照组（n = 2）的少量死后脑脊液样本进行分析。在第1、4和7天收集拉曼光谱，并使用主成分分析（PCA）和均匀流形近似和投影（UMAP）进行分析。结果在SAA后，PCA和UMAP在24 h（第1天）内将合并LBD样本与对照组区分开来，反映了与α-突触核蛋白颤动一致的生化变化。光谱位移表明α-螺旋含量降低，β-薄片结构增加。DLB和PDD之间没有一致的分离。该初步研究表明，将拉曼光谱与机器学习相结合可以实现快速、无标签的疾病特异性变化检测。尽管样本量非常有限，但这些发现强调了这种新工作流程的潜力，并强烈保证其在更大的队列中得到验证。

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引用次数: 0

Detecting abnormal dynamic patterns of phase changes in schizophrenia from complex-valued fMRI data 从复杂值fMRI数据中检测精神分裂症的异常动态相位变化模式。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2025-11-11 DOI: 10.1016/j.jneumeth.2025.110622

Yan-Wei Niu , Qiu-Hua Lin , Jia-Yang Song , Bai-Yu Chen , Vince D. Calhoun

Background

Dynamic analysis has shown advantages in detecting psychiatric-related functional alterations using magnitude-only fMRI data. However, polarity dynamics in source phase derived from complex-valued fMRI data remain largely unexplored, though it offers additional information about brain networks.

New methods

We propose a framework for analyzing source phase polarity dynamics. We first extract dynamic source phase maps using independent component analysis from sliding-window complex-valued fMRI data. Then, we detect voxel-wise polarity changes between adjacent windows and compute their cumulative distributions. Next, we model the distributions with a piecewise linear function under constraints of a minimal segment length and polarity change stability. Finally, we form three dynamic patterns of phase polarity changes via clustering of the model parameters, and obtain pattern-specific sub-regions within activation maps.

Results

We test the proposed framework using complex-valued fMRI data from schizophrenia patients and healthy controls. The dynamic patterns demonstrate that rapid polarity changes occur frequently in the central region of an activation map, occasionally appear in the intermediate region, but rarely occur at the edges. Cluster centroids and the number of voxels within each pattern-specific sub-region show significant group differences.

Comparison with existing methods

Our approach reveals a positively correlated relationship between the average number of polarity changes and activation magnitude, and provides unique sensitivity of the phase polarity to disease-related alterations beyond magnitude-based features.

Conclusions

This work provides new evidence of abnormal dynamic phase patterns in schizophrenia, offering insights into the underlying mechanisms of mental disorders.

背景：动态分析显示了仅使用fMRI数据检测精神相关功能改变的优势。然而，从复杂值的fMRI数据中得出的源相极性动力学仍然很大程度上未被探索，尽管它提供了关于大脑网络的额外信息。新方法：我们提出了一个分析源相极性动力学的框架。我们首先利用独立分量分析从滑动窗口复值fMRI数据中提取动态源相位图。然后，我们检测相邻窗口之间的体素极性变化，并计算它们的累积分布。其次，在最小段长度和极性变化稳定性约束下，用分段线性函数对分布进行建模。最后，通过对模型参数的聚类，形成相极性变化的三种动态模式，并在激活图中获得特定模式的子区域。结果：我们使用来自精神分裂症患者和健康对照者的复杂值fMRI数据来测试所提出的框架。动态模式表明，快速极性变化频繁发生在激活图的中心区域，偶尔出现在中间区域，但很少发生在边缘区域。每个模式特定子区域内的聚类质心和体素数显示出显著的组间差异。与现有方法的比较：我们的方法揭示了极性变化的平均次数与激活幅度之间的正相关关系，并且提供了相位极性对疾病相关变化的独特敏感性，超出了基于幅度的特征。结论：本研究为精神分裂症的异常动态相模式提供了新的证据，为精神障碍的潜在机制提供了见解。

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引用次数: 0

The effects of MEP counts on intra- and intersession reliability of single-pulse TMS-induced MEPs in the wrist extensor muscle of healthy individuals MEP计数对健康个体单脉冲tms诱导的腕伸肌MEP的内、间歇可靠性的影响

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2025-11-10 DOI: 10.1016/j.jneumeth.2025.110621

Hamed Alanazi , Prue Morgan , Shapour Jaberzadeh

Background

Motor evoked potentials (MEPs) exhibit high intra-individual variability, which can compromise reliability. One suggested approach to reduce this variability is averaging multiple MEPs. However, there is no evidence regarding the optimal number of MEPs required to obtain the most reliable amplitude and latency measurements from the extensor carpi radialis (ECR) muscle.

New method

Healthy participants attended two sessions. In the first session, two sets of TMS-induced MEPs were recorded with a 20-minute interval to evaluate intra-session reliability. The second session was conducted after at least 48 h to assess inter-session reliability. Reliability was quantified using intraclass correlation coefficients (ICCs), one-way ANOVA, and Bland–Altman analysis.

Results

For MEP amplitude, both intra- and inter-session ICC values were excellent across all MEP block sizes, with reliability improving as the number of MEPs per block increased. For MEP latency, 10 MEPs were sufficient to achieve excellent intra-session reliability, whereas inter-session reliability ranged from good to excellent, with larger blocks (16–25 MEPs) providing the highest ICC values.

Comparison with existing methods

This study is the first to examine MEP reliability in the ECR muscle. Compared with previous methods, including monophasic TMS in other muscles, our protocol shows that MEP latency in the ECR requires larger MEP blocks to achieve high inter-session reliability.

Conclusions

This study suggests that averaging MEPs from the ECR yields highly reproducible amplitude measurements with few blocks, whereas MEP latency requires more blocks to achieve high inter-session reliability.

背景：运动诱发电位（MEPs）表现出高度的个体内变异性，这可能会损害可靠性。减少这种可变性的一种建议方法是对多个mep取平均值。然而，对于获得桡侧腕伸肌（ECR）最可靠的振幅和潜伏期测量所需的最佳mep数量，尚无证据。新方法：健康参与者参加两次会议。在第一个会话中，记录两组tms诱发的mep，间隔20分钟，以评估会话内可靠性。第二次测试在至少48小时后进行，以评估测试间的可靠性。信度采用类内相关系数（ICCs）、单因素方差分析和Bland-Altman分析进行量化。结果：对于MEP振幅，在所有MEP块大小中，会话内和会话间的ICC值都很好，随着每个MEP块数量的增加，可靠性也在提高。对于MEP延迟，10个MEP足以实现出色的会话内可靠性，而会话间可靠性范围从良好到优秀，较大的块（16-25个MEP）提供最高的ICC值。与现有方法的比较：本研究首次检验了ECR肌MEP的可靠性。与以前的方法（包括其他肌肉的单相经颅磁刺激）相比，我们的方案表明，ECR中的MEP延迟需要更大的MEP块来实现高会话间可靠性。结论：本研究表明，从ECR中平均MEP可以产生高度可重复的振幅测量，只需很少的块，而MEP延迟需要更多的块来实现高会话间可靠性。

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引用次数: 0

High-density multielectrode array (MEA) recordings in a rodent glioma model 高密度多电极阵列（MEA）在啮齿动物胶质瘤模型中的记录。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2025-11-09 DOI: 10.1016/j.jneumeth.2025.110623

Ferdinand Forberger , Fabiana Santana Kragelund , Katrin Porath , Rüdiger Köhling , Falko Lange , Timo Kirschstein

Background

High-grade gliomas are aggressive primary brain tumors that affect the surrounding brain tissue, which can lead to neurological deficits, epilepsy, cognitive deficits, and tumor progression. Research has focused on glutamatergic alterations, but the understanding of the underlying network mechanisms is still scarce.

New method

To investigate how gliomas might affect neural network integrity and capacity for spiking activity, we induced network bursting events in neocortical slices from F98 glioma-bearing rats. Network activity was recorded using a 64 × 64 high-density multielectrode array to assess the network's capacity for extended periods of high activity. We describe glioma cell implantation, brain slice preparation, and detailed data analysis of electrophysiological recordings, focusing on computational methods for characterization of the network-wide bursting events.

Results

Spike sorting was performed using Kilosort4 and employed to identify network bursts. Burst origins were estimated from multi-unit activity, and network burst propagation speed was assessed. Network-level functional connectivity was analyzed using metrics derived from graphs based on the spike time tiling coefficient. Our results indicate reduced network integrity in glioma-bearing slices.

Comparison with existing methods

The combination of a high-density multielectrode array with Kilosort4 is advantageous over conventional recording techniques in terms of detecting network bursting and plotting center-of-activity trajectories.

Conclusions

This protocol provides a robust platform for dissecting network activity and establishes a basis for future mechanistic studies. We also discuss the limitations and experimental challenges of our preclinical approach to investigating glioma-associated effects on surrounding neuronal networks.

背景：高级别胶质瘤是侵袭性原发性脑肿瘤，影响周围脑组织，可导致神经功能障碍、癫痫、认知障碍和肿瘤进展。研究集中在谷氨酸能的改变上，但对潜在的网络机制的理解仍然很少。新方法：为了研究胶质瘤如何影响神经网络的完整性和尖峰活动的能力，我们在F98胶质瘤大鼠的新皮质切片中诱导网络破裂事件。使用64×64高密度多电极阵列记录网络活动，以评估网络长时间高活动的能力。我们描述了神经胶质瘤细胞植入、脑切片制备和电生理记录的详细数据分析，重点是描述网络范围内爆发事件的计算方法。结果：使用Kilosort4进行峰值排序，并用于识别网络突发。根据多单元活动估计突发源，评估网络突发传播速度。使用基于峰值时间平铺系数的图派生的度量来分析网络级功能连通性。我们的结果表明，神经胶质瘤切片的网络完整性降低。与现有方法的比较：多电极阵列与Kilosort4相结合在检测网络爆发和绘制活动中心轨迹方面优于传统的记录技术。结论：该协议为剖析网络活动提供了一个强大的平台，并为未来的机制研究奠定了基础。我们还讨论了研究胶质瘤对周围神经网络相关影响的临床前方法的局限性和实验挑战。

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引用次数: 0

Impact of buccal and marginal mandibular branch transection on neuromuscular junction integrity in a rodent model of facial nerve paralysis 颊支和下颌边缘支横断对面神经麻痹啮齿动物神经肌肉连接完整性的影响。

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods

Pub Date : 2025-11-07 DOI: 10.1016/j.jneumeth.2025.110620

Barbara G. Marin , Jordan L. Grant , Christine Mei , Giancarlo Tejeda , Ruby R. Taylor , Grant M. Lee , Victoria L. Ortega , Isabella G. Cozzone , Rachael M. Robbio , Trinity Robbio , Abhishek Prasad , Shekhar K. Gadkaree , Courtney M. Dumont

Background

Facial nerve paralysis is a complex and devastating condition, with translational research primarily relying on animal models. Models include injuries to the main trunk or distal branches of the facial nerve, such as the buccal and marginal mandibular branches. While recovery is well described following main trunk injury, this study investigates the impact of distal branch injuries on facial nerve recovery and neuromuscular junction (NMJ) innervation.

New method

Adult rats underwent transection of the buccal branch (BB) or of the buccal and marginal mandibular branches (BB+MM) of the facial nerve and were compared to sham controls. Facial palsy scores, compound muscle action potentials (CMAP), and NMJ innervation were quantified to assess chronic paralysis.

Results

BB transection alone did not result in prolonged facial palsy, as facial palsy scores and CMAPs remained comparable to sham conditions. However, BB+MM transection reduced NMJ innervation by 50 % and induced whisker and nose paralysis. Pre- and post-synaptic morphology metrics were significantly reduced in the BB+MM group, whereas the BB group NMJs resembled the morphology to the NMJs in the sham group.

Comparison with existing methods

These results expand existing studies by providing quantitative insights into NMJ innervation and demonstrating the complete loss of nasal mobility following the combined distal branch injury model.

Conclusions

These results provide further model characterization of distal facial nerve branches contribution to NMJ integrity and nasal mobility. This model will serve as a testbed for future biomaterials in facial nerve injury, incorporating comprehensive characterization techniques to assess repair and function.

背景：面神经麻痹是一种复杂且具有破坏性的疾病，其转化研究主要依赖于动物模型。模型包括面神经主干或远端分支的损伤，如颊支和下颌边缘分支。虽然主干损伤后的恢复有很好的描述，但本研究探讨了远端分支损伤对面神经恢复和神经肌肉连接（NMJ）神经支配的影响。新方法：对成年大鼠进行面神经颊支（BB）或颊支和下颌边缘支（BB+MM）的横断，并与假手术对照组进行比较。面瘫评分、复合肌动作电位（CMAP）和NMJ神经支配被量化以评估慢性麻痹。结果：单独的BB横断不会导致长时间的面瘫，因为面瘫评分和CMAPs与假手术条件相当。然而，BB+MM横断使NMJ神经支配减少50%，并引起须和鼻麻痹。BB+MM组突触前和突触后形态学指标显著降低，而BB组NMJs的形态学与假手术组的NMJs相似。与现有方法的比较：这些结果扩展了现有的研究，提供了NMJ神经支配的定量见解，并证明了联合远端分支损伤模型后鼻活动完全丧失。结论：这些结果为面神经远端分支对NMJ完整性和鼻活动的贡献提供了进一步的模型表征。该模型将作为未来面神经损伤生物材料的测试平台，结合综合表征技术来评估修复和功能。

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引用次数: 0