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Antioxidant activity, total phenolic and flavonoid content determination of some selected medicinal plants from Kavre District of Nepal 尼泊尔Kavre地区部分药用植物抗氧化活性、总酚和类黄酮含量测定
Pub Date : 2020-02-20 DOI: 10.3126/jncs.v38i0.27786
R. Kharel, K. Sharma
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引用次数: 0
Anti-inflammatory Compounds Camphor and Methylsalicylate from Traditionally Used Pain Curing Plant Equisetum arvense L. 传统镇痛植物木贼中的抗炎化合物樟脑和甲基水杨酸。
Pub Date : 2019-12-31 DOI: 10.3126/jncs.v40i0.27270
A. Adhikari, S. Bhandari, D. Pandey
Equiseum arvense L.  is a bushy perennial herb commonly called field horsetail distributed in the northern part of Nepal and used against curing muscle and bone pain and other many diseases. This research work was performed to analyze the possible cause of the anti-inflammatory activity of this plant. Out of four different fractions, dichloromethane (DCM ) fraction showed the presence of compounds on TLC and was subjected to column chromatography to isolate and identify pure compounds. D2 and D20 fractions eluted from column gave single and distinguished spots so that these fractions were subjected to gas chromatography analysis. The GC analysis of the pure fraction D2 obtained from column revealed the presence of a major compound major compounds camphor (92.53%) and other minor compounds; (+)-2-Bornanone, Camphor, Carvone<(z)-, dihydro->, Benzoic acid, 2-(1-oxopropyl)- and GC report from D20 fraction showed two compounds; Salicylate and  Benzoic acid, 2-(1-oxopropyl)-. The major compounds camphor (92.53%) and methyl salicylate (66.87%) have been reported being potent anti-inflammatory activity and their synergetic effect is more prominent for the same.  
马尾草(Equiseum arvense L.)是一种多年生灌木草本植物,俗称野马尾,分布在尼泊尔北部,用于治疗肌肉和骨骼疼痛等多种疾病。本研究旨在分析该植物抗炎活性的可能原因。在4个不同的馏分中,二氯甲烷(DCM)馏分在TLC上显示出化合物的存在,并进行了柱层析分离和鉴定纯化合物。从柱中洗脱的D2和D20组分具有单点和区分点,因此这些组分可以进行气相色谱分析。色谱分析表明,D2中含有主要化合物,主要化合物樟脑(92.53%)和少量化合物;(+)-2-龙骨酮、樟脑、香芹酮、苯甲酸、2-(1-氧丙基)-和D20馏分的GC报告显示两种化合物;水杨酸和苯甲酸,2-(1-氧丙基)-。主要化合物樟脑(92.53%)和水杨酸甲酯(66.87%)具有较强的抗炎活性,其协同作用更为突出。
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引用次数: 3
Extraction of Silica Nanoparticles from Rice Husk Ash (RHA) and Study of Its Application in Making Composites 稻壳灰中二氧化硅纳米颗粒的提取及其复合材料制备研究
Pub Date : 2019-12-31 DOI: 10.3126/jncs.v40i0.27285
Bimal Kumar Raut, Khim Prasad Panthi
Nanotechnology is the most emerging field in the area of different scientific research. Various methods of synthesis of nanoparticles are available. The wet chemical synthesis method is applicable in the extraction of silica nanoparticles from Rice Husk Ash (RHA). Rice husk is a form of waste product from the rice milling industries which is produced in an abundance amount in and around the country. Rice husk which is generally left on the field as a waste contains 60% silica content and can be economically viable raw materials for the extraction of silica [1-2]. Initially, Rice husk was burnt to obtain its ash. Then, a simple bottom-up approach, the sol-gel method was applied, and fine powder silica in the nanoscopic range was extracted. After extraction of it, internal arrangement and average particle size were recognized by XRD while molecular components and structure present in silica were identified by FTIR. The obtained silica was then used in making of ceramics matrix nanocomposites (particularly silica-sand cement block), and its mechanical properties were identified by compressive strength test using Instron testing machine which was found to be increased in comparing with the compressive strength of ceramics composite (Sand cement block) prepared in the same ratio and same size of mold as that of ceramics matrix nanocomposites. The ceramics matrix nanocomposites acquiring higher mechanical strength than Ceramics composite occurred due to the incorporation of Silica nanoparticles.
纳米技术是不同科学研究领域中最新兴的领域。纳米颗粒的合成方法多种多样。湿法化学合成法适用于稻壳灰(RHA)中二氧化硅纳米颗粒的提取。稻壳是碾米业产生的一种废料,在国内及周边地区大量生产。稻壳通常作为废弃物留在田间,其二氧化硅含量为60%,可作为提取二氧化硅的经济可行原料[1-2]。最初,稻壳被焚烧以获得稻壳的灰。然后,采用一种简单的自下而上的方法,即溶胶-凝胶法,提取纳米范围内的细粉二氧化硅。提取后用XRD分析了二氧化硅的内部排列和平均粒径,用FTIR分析了二氧化硅的分子组成和结构。将所得的二氧化硅用于制备陶瓷基纳米复合材料(特别是硅砂水泥块),并利用Instron试验机对其进行抗压强度测试,其力学性能与相同配比、相同模具尺寸制备的陶瓷基纳米复合材料(砂水泥块)相比,抗压强度有所提高。陶瓷基纳米复合材料获得比陶瓷复合材料更高的机械强度是由于二氧化硅纳米颗粒的掺入。
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引用次数: 3
Phytochemical Screening and Cytotoxicity Evaluation of Ethanolic Extract of Hypericum cordifolium (choisy) Leaves 金丝桃叶乙醇提取物的植物化学筛选及细胞毒性评价
Pub Date : 2019-12-31 DOI: 10.3126/jncs.v40i0.27273
Deepak Basyal, N. L. Bhandari
Hypericum cordifolium is a medicinal plant traditionally used for the treatment of the menstrual disorder, backache, dislocation of bone, fever, diarrhea and dysentery due to the presence of potential bioactive secondary metabolites. Ethanolic extract of dried leaf powder was prepared using the Soxhlet apparatus, followed by qualitative phytochemical screening with different reagents. In-vitro cytotoxicity assay was screened by using brine shrimp bioassay and LC50 was calculated by probit analysis. Alkaloids, glycosides, amino acids, and fixed oils were present at very minute level whereas saponin, phenol and flavonoids were present at significant level. Cytotoxicity assay of the leaf extract showed significant results (p<0.05).LC50   of 273 ppm, which was less than 1000 ppm, showing that the plant is toxic. The study revealed that the plant possessed medicinal values, which could justify the ethno-medicinal importance scientifically. This research has pointed out further possibilities of works to be done on this virgin plant creating the opportunity for scientific researches to give birth of potent lead molecules.
金丝桃是一种药用植物,传统上用于治疗月经紊乱、背痛、脱臼、发烧、腹泻和痢疾,因为存在潜在的生物活性次级代谢物。采用索氏装置制备干叶粉末乙醇提取物,并用不同试剂进行定性植物化学筛选。采用卤虾生物测定法筛选体外细胞毒性试验,probit分析法计算LC50。生物碱、糖苷、氨基酸和固定油含量极低,皂苷、酚和黄酮类化合物含量极显著。叶提取物细胞毒性试验结果显著(p<0.05)。LC50为273 ppm,小于1000 ppm,说明该植物有毒。研究结果表明,该植物具有药用价值,可以从科学上证明其民族药用价值。这项研究指出了在这种原生植物上进一步开展工作的可能性,为科学研究产生强效铅分子创造了机会。
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引用次数: 4
Corrosion Inhibition of Bark Extract of Euphorbia royleana on Mild Steel in 1M HCl 大戟皮提取物在1M HCl中对低碳钢的缓蚀作用
Pub Date : 2019-12-31 DOI: 10.3126/jncs.v40i0.27274
B. Thapa, D. Gupta, A. Yadav
The bark extract of Euphorbia royleana as a green corrosion inhibitor was studied in 1M HCl using weightloss method and potential measurement. The results show that the bark extract of Euphorbia royleana is an effective anti-corrosion inhibitor of mild steel in acidic media. The corrosion rate decreases with the time of immersion. Weight loss experiment shows that the loss in weight decreases with the time of immersion and inhibition efficiency increases with the concentration of extract. It was observed that maximum inhibition efficiency is 99.60% in 100% concentration of extract. Potential measurement study shows that bark extracts act as a mixed type of inhibitor i.e. inhibits both anodically as well as cathodically.
采用失重法和电位法对大戟树皮提取物作为绿色缓蚀剂在1M HCl中进行了研究。结果表明,大戟树皮提取物在酸性介质中是一种有效的低碳钢缓蚀剂。腐蚀速率随浸泡时间的延长而减小。失重实验表明,浸提液的失重随浸提时间的延长而减小,抑制效果随浸提液浓度的增加而增大。结果表明,在100%浓度下,其抑菌率可达99.60%。电位测量研究表明,树皮提取物作为一种混合型抑制剂,即既有阳极抑制作用,又有阴极抑制作用。
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引用次数: 13
Electrospun Spider-net Structured Nanofibers Membrane from Homogeneous Solution of Nylon-6 and Poly (Ethylene oxide) 尼龙-6 -聚环氧乙烷均相溶液的电纺蛛网结构纳米纤维膜
Pub Date : 2019-12-31 DOI: 10.3126/jncs.v40i0.27282
M. Jha, D. Shah, K. Dhital, L. R. Bhatta, S. Joshi, R. K. Sharma, H. Pant
Membrane development encompasses wide range of technology areas including process and product design, materials engineering, chemical engineering, as well as interaction phenomenon. Advances in membrane technology can solve most of the global concerns related to water, air, energy, healthcare and global warming. In this study, a simple electrospinning technique was applied to prepare composite nanofibers from Nylon-6 and polyethylene oxide (PEO) blend solutions. The effect of PEO on the morphology of the fiber was investigated. It was observed that the addition of PEO in the Nylon-6 solution resulted in the formation of ultrafine nanofibers along with main the fiber, which can be considered as a spider-net-like morphology. The nano/sub-nano arrangement of the fiber resembling a three-dimensional (3D) spider-net structure enhances the mechanical strength of the resulting nanofibers as compared to the pristine Nylon-6 nanofibers.
膜的开发涉及广泛的技术领域,包括工艺和产品设计、材料工程、化学工程以及相互作用现象。膜技术的进步可以解决大多数与水、空气、能源、医疗保健和全球变暖有关的全球问题。在本研究中,采用简单的静电纺丝技术制备了尼龙-6和聚乙烯氧化物(PEO)共混溶液的复合纳米纤维。研究了PEO对纤维形貌的影响。结果表明,在尼龙-6溶液中加入PEO后,纳米超细纤维与主要纤维一起形成网状结构。与原始的尼龙-6纳米纤维相比,纤维的纳米/亚纳米排列类似于三维(3D)蜘蛛网结构,从而提高了纳米纤维的机械强度。
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引用次数: 0
Dielectric Phase Transition Behaviour Study of Dry Route Derived (BA0.5 SN0.5) TIO3 Ceramics 干路衍生(BA0.5 SN0.5) TIO3陶瓷介电相变行为研究
Pub Date : 2019-12-31 DOI: 10.3126/jncs.v40i0.27286
B. Bhandari, Bhadra Prasad Pokheral
Ceramic materials display a wide range of properties that facilitate their use in many different product areas. Currently, there has been keen interest in the field of ceramic materials due to their excellent mechanical and physical properties. Barium Stannate Titanate (BST) is a binary solid solution system composed of ferroelectric Barium titanate and non-ferroelectric barium titanate. In this study, the phase transition behavior of (Ba1-xSnx) TiO3 (x = 0.5) (BST) ceramics was obtained by the dry-route method. The previous studies were based on Sn2+ on the Ti site with varying values of x. The powders after calcination are compacted in the form of pellets using a hydraulic press at an optimized load above 70 KN. The experimental density of our sample measured by liquid displacement method with glycerin was lower than theoretical density, giving the shape is highly dense with low porosity. The structure shows that on increasing the Sn2+ content volume decreases due to the size of Sn2+, which is smaller than that of Ba2+, in comparison to BaTiO3. As the demand of lead-free environment-friendly sensor is increasing, thus obtained BST has great applications as a sensor material in modern electronic devices.
陶瓷材料显示出广泛的特性,这有利于它们在许多不同的产品领域的使用。陶瓷材料以其优异的机械性能和物理性能引起了人们的极大兴趣。锡钛酸钡(BST)是由铁电性钛酸钡和非铁电性钛酸钡组成的二元固溶体体系。本研究采用干法研究了(Ba1-xSnx) TiO3 (x = 0.5) (BST)陶瓷的相变行为。先前的研究是基于不同x值的Ti位点上的Sn2+。煅烧后的粉末在70 KN以上的优化负载下使用液压机压实成球团形式。用甘油液体置换法测得的样品实验密度低于理论密度,具有高密度、低孔隙率的形状。结构表明,随着Sn2+含量的增加,Sn2+的体积比BaTiO3小,这是由于Sn2+的体积比Ba2+小。随着人们对无铅环保传感器的需求日益增加,由此获得的BST作为一种传感器材料在现代电子器件中有着巨大的应用前景。
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引用次数: 0
Fabrication and Characterization of Starch-Based Biodegradable Polymer with Polyvinyl Alcohol 淀粉基聚乙烯醇可生物降解聚合物的制备与表征
Pub Date : 2019-12-31 DOI: 10.3126/jncs.v40i0.27283
Shanta Pokhrel, L. Rai
Starch is a renewable, biodegradable and low-cost natural biopolymer with high availability. Starch was extracted from Solanum tuberosum (potatoes) and its preliminary properties were studied. The structure of extracted starch was characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). A series of starch-based biodegradable blends, with polyvinyl alcohol (PVA) were prepared by using glycerol as a plasticizer and citric acid as a cross-linking agent, through solution casting method and their biodegradable properties were studied. The structure of the blend was characterized by Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimeter (DSC). The morphology of starch shows the potato starch has oval or ellipsoid in the structure having a size of 20-35 µm. The compatibility of the two components can be improved by the hydroxyl group present on both starch and PVA. As compared to pure polyvinyl, the melting point of the blend was decreased. The blends were found degradable within a weak.
淀粉是一种可再生、可生物降解、低成本的天然生物高聚物。从马铃薯中提取淀粉,并对淀粉的性质进行初步研究。利用傅里叶红外光谱(FTIR)和扫描电镜(SEM)对提取淀粉的结构进行了表征。以甘油为增塑剂,柠檬酸为交联剂,通过溶液浇铸法制备了一系列以聚乙烯醇(PVA)为原料的淀粉基可降解共混物,并对其生物降解性能进行了研究。用傅里叶红外光谱(FTIR)和差示扫描量热仪(DSC)对共混物的结构进行了表征。淀粉的形貌表明,马铃薯淀粉的结构为椭圆形或椭圆体,尺寸为20 ~ 35µm。淀粉和聚乙烯醇上的羟基可以改善两组分的相容性。与纯聚氯乙烯相比,共混物的熔点降低了。混合物被发现在一个弱。
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引用次数: 2
Assessment of Total Phenolic, Flavonoid Content and Antioxidant Activity of Ocimum sanctum Linn 山茱萸总酚、类黄酮含量及抗氧化活性的研究
Pub Date : 2019-12-31 DOI: 10.3126/jncs.v40i0.27275
Ishwor Pathak, Muna Niraula
Ocimum sanctum, commonly known as Tulasi in Nepal, is a pharmacologically important plant due to its active constituents. In this work, extraction was carried out in hexane, chloroform and methanol solvents and their phytochemical screening was performed. Total phenolic and flavonoid contents in the plant were measured by Folin-Ciocalteu colorimetric method and aluminum chloride colorimetric method respectively. Antioxidant activity of the extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Alkaloids, flavonoids, tannins, glycosides, polyphenols, terpenoids, tannins and steroids are mainly found in the extracts. Based on the result obtained, the plant posses a significant amount of total phenolic and total flavonoid content. Both phenolic and flavonoid contents were highest in methanol extract, followed by chloroform and hexane extract. Antioxidant activity of the extracts as ascorbic acid standard (IC50 value = 41.34 µg/mL) was in the order of methanol extract (IC50 value = 47.73 µg/mL) > chloroform extract (IC50 value = 79.46 µg/mL) > hexane extract (IC50 value = 94.68 µg/mL). The extent of the antioxidant activity of the plant is following the number of total phenolics and flavonoids present in it.
藏红花,尼泊尔俗称图拉西,因其有效成分而成为一种重要的药理学植物。本研究分别在己烷、氯仿和甲醇溶剂中提取,并对其进行了植物化学筛选。采用福林比色法和氯化铝比色法分别测定植物中总酚和总黄酮的含量。采用1,1-二苯基-2-苦味酰肼(DPPH)自由基清除法评价提取物的抗氧化活性。提取物中主要含有生物碱、黄酮类、单宁、糖苷、多酚、萜类、单宁和类固醇。根据得到的结果,该植物具有大量的总酚和总黄酮含量。甲醇提取物中酚类和类黄酮含量最高,其次为氯仿和己烷提取物。抗坏血酸标准提取物的抗氧化活性依次为甲醇提取物(IC50值= 47.73µg/mL)、氯仿提取物(IC50值= 79.46µg/mL)、己烷提取物(IC50值= 94.68µg/mL)。植物的抗氧化活性的程度是随着总酚类物质和黄酮类物质的数量而变化的。
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引用次数: 7
Physico-chemical Parameterization and Determination of Effect of Tributaries on Enhancement of Pollutants in Bagmati River 巴格玛提河支流对污染物增强影响的理化参数化及测定
Pub Date : 2019-12-31 DOI: 10.3126/jncs.v40i0.27276
M. Adhikari, Madhav Neupane, M. Kafle
Water quality parameterization is a great concern because chemical contaminants and microbiological impurities including pathogenic bacteria may pose a health risk and unfit for its domestic use. Alkalinity, pH, conductivity, chlorine demand, turbidity, and ammonia were measured to characterize the water quality of the Bagmati River. The effect of tributaries on Bagmati River was determined by sampling water from five different sites such as Pashupati (B-1), Shankhamul (B-2), Kupondol (B-1), Balkhu (B-4), and Jalbinayek (B-5) sites. The water samples B-2, B-3, B-4 and B-5 were less turbid but black in color while water sample B-1 was more turbid but grey in color. The pH of water samples ranged from 6.7 to 7.3. The alkalinity, conductivity and chlorine demand were 60 ppm, 95.7 µs and, 5.44 ppm, respectively for B-1 sample and increased almost continuously from B-2 to B-5 sample. The alkalinity was 360 ppm, conductivity was 862 µs and chlorine demand was 23.7 ppm for the last sample (B-5). The concentration of ammonia in the B-1 sample was only 0.0625 ppm whereas it was 3.32 ppm in the B-5 sample. The enhancement of chlorine demand and concentration of ammonia attributed that tributary and local effluent loaded extremely high levels of pollutants into the Bagmati River which might include pathogenic microorganisms. The random chance of some parameters like pH, conductivity, ammonia, turbidity along the Bagmati River indicates the impacts of different tributaries on Bagmati River. The chorine demand showed a positive correlation with conductivity, alkalinity and ammonia while the negative correlation with turbidity. This revealed that the conductive alkaline pollutants consumed more chlorine than colloidal particles. From the study it is considered that the Bagmati River water contains natural as well as anthropogenic pollutants which is extremely hazardous not only to the people using river water but also for the living organism rely on the river. 
水质参数化是一个非常值得关注的问题,因为化学污染物和包括致病菌在内的微生物杂质可能对健康构成威胁,不适合在家庭使用。测定了巴格马提河的碱度、pH值、电导率、氯需要量、浊度和氨的水质特征。支流对巴格马提河的影响是通过从5个不同的地点,如帕舒帕蒂(B-1)、尚卡穆尔(B-2)、库蓬多(B-1)、巴尔胡(B-4)和贾尔比纳耶克(B-5)取样来确定的。水样B-2、B-3、B-4、B-5浑浊度较小,呈黑色;水样B-1浑浊度较大,呈灰色。水样pH值为6.7 ~ 7.3。B-1样品的碱度、电导率和需氯量分别为60 ppm、95.7µs和5.44 ppm,从B-2到B-5样品几乎连续增加。最后一个样品(B-5)的碱度为360 ppm,电导率为862µs,氯需求量为23.7 ppm。B-1样品中的氨浓度仅为0.0625 ppm,而B-5样品中的氨浓度为3.32 ppm。氯需求和氨浓度的增加是由于支流和当地污水向巴格马提河排放了极高水平的污染物,其中可能包括致病微生物。巴格马提河沿岸pH值、电导率、氨、浊度等参数的随机变化表明了不同支流对巴格马提河的影响。氯需要量与电导率、碱度、氨含量呈正相关,与浊度呈负相关。这表明导电碱性污染物比胶体颗粒消耗更多的氯。研究认为,巴格玛提河水中既有天然污染物,也有人为污染物,这不仅对使用河水的人,而且对依赖河水的生物都是极其危险的。
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引用次数: 6
期刊
Journal of Nepal Chemical Society
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