TiO2 and ZnO nanoparticles (NPs) were synthesized using microwave-assisted method. Synthesized NPs were characterized for their structure, morphology, and elemental composition using X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). The crystallite size of synthesized NPs of TiO2 and ZnO was about 12.3 and 18.7 nm as obtained from the Scherrer formula from the most intense XRD peak. The synthesized NPs have been found to be in stoichiometric ratio having anatase and hexagonal wurtzite structure for TiO2 and ZnO, respectively, and are spherical in shape. Surface area of TiO2 and ZnO NPs was found to be about 43.52 m2/g and 7.7 m2/g. Photocatalytic (PC) properties of synthesized NPs were studied for malachite green (MG) dye under UV light. TiO2 NPs were found to be highly photocatalytically active among the two, having efficiency and apparent photodegradation rate of 49.35% and , respectively.
{"title":"Photocatalytic Properties of Microwave-Synthesized TiO2 and ZnO Nanoparticles Using Malachite Green Dye","authors":"Ashutosh Kumar Singh, U. Nakate","doi":"10.1155/2013/310809","DOIUrl":"https://doi.org/10.1155/2013/310809","url":null,"abstract":"TiO2 and ZnO nanoparticles (NPs) were synthesized using microwave-assisted method. Synthesized NPs were characterized for their structure, morphology, and elemental composition using X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). The crystallite size of synthesized NPs of TiO2 and ZnO was about 12.3 and 18.7 nm as obtained from the Scherrer formula from the most intense XRD peak. The synthesized NPs have been found to be in stoichiometric ratio having anatase and hexagonal wurtzite structure for TiO2 and ZnO, respectively, and are spherical in shape. Surface area of TiO2 and ZnO NPs was found to be about 43.52 m2/g and 7.7 m2/g. Photocatalytic (PC) properties of synthesized NPs were studied for malachite green (MG) dye under UV light. TiO2 NPs were found to be highly photocatalytically active among the two, having efficiency and apparent photodegradation rate of 49.35% and , respectively.","PeriodicalId":16507,"journal":{"name":"Journal of Nanoparticles","volume":"13 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2013-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84979037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A surfactant/solid-template-free hydrothermal process has been developed for the synthesis of single-crystalline nanorods of bismuth sulfide (Bi2S3) using triethanolamine as a complexing agent for the Bi3
以三乙醇胺为络合剂，建立了一种无表面活性剂/固体模板的水热法制备硫化铋(Bi2S3)单晶纳米棒
{"title":"The Growth of Bismuth Sulfide Nanorods from Spherical-Shaped Amorphous Precursor Particles under Hydrothermal Condition","authors":"Pravas Kumar Panigrahi, A. Pathak","doi":"10.1155/2013/367812","DOIUrl":"https://doi.org/10.1155/2013/367812","url":null,"abstract":"A surfactant/solid-template-free hydrothermal process has been developed for the synthesis of single-crystalline nanorods of bismuth sulfide (Bi2S3) using triethanolamine as a complexing agent for the Bi3","PeriodicalId":16507,"journal":{"name":"Journal of Nanoparticles","volume":"54 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2013-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79424648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZnO nanostructures have been successfully prepared by a microwave irradiation method. The role of albumen as a template in addressing the size and morphology of ZnO has been investigated by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TG-DTA), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) techniques. A heterogeneous mixture of Zn(OH)2 and ZnO was obtained in absence of albumen. Pure ZnO nanostructures, consisting of rod- and whisker-like nanoparticles embedded in a sheet matrix, were obtained in the presence of albumen. Optical and photoluminescence (PL) properties of the synthesized samples were also compared. Results obtained indicate that the microwave-assisted method is a promising low temperature, cheap, and fast method for the production of ZnO nanostructures.
{"title":"Synthesis of ZnO Nanostructures by Microwave Irradiation Using Albumen as a Template","authors":"T. Prakash, R. Jayaprakash, G. Neri, Sanjay Kumar","doi":"10.1155/2013/274894","DOIUrl":"https://doi.org/10.1155/2013/274894","url":null,"abstract":"ZnO nanostructures have been successfully prepared by a microwave irradiation method. The role of albumen as a template in addressing the size and morphology of ZnO has been investigated by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TG-DTA), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) techniques. A heterogeneous mixture of Zn(OH)2 and ZnO was obtained in absence of albumen. Pure ZnO nanostructures, consisting of rod- and whisker-like nanoparticles embedded in a sheet matrix, were obtained in the presence of albumen. Optical and photoluminescence (PL) properties of the synthesized samples were also compared. Results obtained indicate that the microwave-assisted method is a promising low temperature, cheap, and fast method for the production of ZnO nanostructures.","PeriodicalId":16507,"journal":{"name":"Journal of Nanoparticles","volume":"61 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2013-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81119465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Metallic nanocomposites and nanotubes can be rapidly prepared under microwave irradiation (MW) from an aqueous solution of metallic precursors in the presence of trisodium citrate as a reductant. For the nanotubes nanoparticles are stabilized by poly(N-vinyl-2-pyrrolidone) (PVP), a protecting agent. PVP is a typical capping and structure-directing agent used for the synthesis of various metallic nanostructures. In this work, we have demonstrated for the first time an MW irradiation approach for the synthesis of trimetallic nanocomposites and nanotubes. The resulting nanohybrids were characterized by UV-Vis spectroscopic analysis, high-resolution scanning electron microscopy (HR-SEM), energy dispersive X-ray spectroscopic analysis (EDX), and X-ray diffractometer (XRD) techniques.
{"title":"Rapid Green Synthetic Protocol for Novel Trimetallic Nanoparticles","authors":"B. Karthikeyan, B. Loganathan","doi":"10.1155/2013/168916","DOIUrl":"https://doi.org/10.1155/2013/168916","url":null,"abstract":"Metallic nanocomposites and nanotubes can be rapidly prepared under microwave irradiation (MW) from an aqueous solution of metallic precursors in the presence of trisodium citrate as a reductant. For the nanotubes nanoparticles are stabilized by poly(N-vinyl-2-pyrrolidone) (PVP), a protecting agent. PVP is a typical capping and structure-directing agent used for the synthesis of various metallic nanostructures. In this work, we have demonstrated for the first time an MW irradiation approach for the synthesis of trimetallic nanocomposites and nanotubes. The resulting nanohybrids were characterized by UV-Vis spectroscopic analysis, high-resolution scanning electron microscopy (HR-SEM), energy dispersive X-ray spectroscopic analysis (EDX), and X-ray diffractometer (XRD) techniques.","PeriodicalId":16507,"journal":{"name":"Journal of Nanoparticles","volume":"108 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2013-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79171052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The growth of uniformly distributed and densely packed array of zinc oxide (ZnO) nanorods (NRs) and nanorods (NRs)/nanopolypods (NPPs) was successfully achieved through microwave-assisted chemical route at low temperature. The ZnO NRs and NRs/NPPs were characterized using X-ray diffraction (XRD), scanning electron microscope (SEM), energy dispersive X-ray analysis (EDX), and UV-Vis absorption spectroscopy. The ZnO NRs were of 100–150 nm diameter and 0.5–1 μm length, while the NPPs were of diameter about 150–200 nm and 1.5–2 μm pod length. The prepared films are polycrystalline in nature and highly oriented along (002) plane with a hexagonal wurtzite structure. These films were studied for the sensing properties of liquefied petroleum gas (LPG), oxygen, and hazardous explosives, that is, 2,4,6-trinitrotoluene (TNT) and cyclotrimethylenetrinitramines (RDX), in the temperature ranges of 25–425 °C and 100–200 °C, respectively. The grown nanostructure films showed reliable stable response to several on-off cycles, and reduction in sensor recovery time was found with the increase in temperature. ZnO NRs and NRs/NPPs showed better sensitivity and recovery time for both LPG and oxygen, as compared to the literature-reported results for ZnO thin films.
{"title":"Microwave Assisted Growth of ZnO Nanorods and Nanopolypods Nanostructure Thin Films for Gas and Explosives Sensing","authors":"A. Singh","doi":"10.1155/2013/783691","DOIUrl":"https://doi.org/10.1155/2013/783691","url":null,"abstract":"The growth of uniformly distributed and densely packed array of zinc oxide (ZnO) nanorods (NRs) and nanorods (NRs)/nanopolypods (NPPs) was successfully achieved through microwave-assisted chemical route at low temperature. The ZnO NRs and NRs/NPPs were characterized using X-ray diffraction (XRD), scanning electron microscope (SEM), energy dispersive X-ray analysis (EDX), and UV-Vis absorption spectroscopy. The ZnO NRs were of 100–150 nm diameter and 0.5–1 μm length, while the NPPs were of diameter about 150–200 nm and 1.5–2 μm pod length. The prepared films are polycrystalline in nature and highly oriented along (002) plane with a hexagonal wurtzite structure. These films were studied for the sensing properties of liquefied petroleum gas (LPG), oxygen, and hazardous explosives, that is, 2,4,6-trinitrotoluene (TNT) and cyclotrimethylenetrinitramines (RDX), in the temperature ranges of 25–425 °C and 100–200 °C, respectively. The grown nanostructure films showed reliable stable response to several on-off cycles, and reduction in sensor recovery time was found with the increase in temperature. ZnO NRs and NRs/NPPs showed better sensitivity and recovery time for both LPG and oxygen, as compared to the literature-reported results for ZnO thin films.","PeriodicalId":16507,"journal":{"name":"Journal of Nanoparticles","volume":"26 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2013-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82227601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kuan-Jen Chen, F. Hung, T. Lui, Cheng-Hung Chen, Sheng-Po Chang
This study synthesizes powders using an aqueous solution method. The powders with different content ratios of CuO and ZnO (CuO : ZnO = 1 : 2, 1 : 1, and 2 : 1) were formed. The crystalline characteristics and electrical and magnetic properties depended primarily on the mixing effect and oxygenation. The electrical resistance of C0.5Z0.5O () powder was lower than that of CuO () powder after ZnO mixing in CuO. This reduction was attributed to the substitution of Cu
{"title":"The Influences of CuO/ZnO Ratios on the Crystallization Characteristics Electrical and Magnetic Properties of Powders","authors":"Kuan-Jen Chen, F. Hung, T. Lui, Cheng-Hung Chen, Sheng-Po Chang","doi":"10.1155/2013/405043","DOIUrl":"https://doi.org/10.1155/2013/405043","url":null,"abstract":"This study synthesizes powders using an aqueous solution method. The powders with different content ratios of CuO and ZnO (CuO : ZnO = 1 : 2, 1 : 1, and 2 : 1) were formed. The crystalline characteristics and electrical and magnetic properties depended primarily on the mixing effect and oxygenation. The electrical resistance of C0.5Z0.5O () powder was lower than that of CuO () powder after ZnO mixing in CuO. This reduction was attributed to the substitution of Cu","PeriodicalId":16507,"journal":{"name":"Journal of Nanoparticles","volume":"599 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2013-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76785949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microwave-assisted organic synthesis, a green chemistry approach, is nowadays widely used in the drug synthesis. Microwave-assisted synthesis improves both throughput and turnaround time for medicinal chemists by offering the benefits of drastically reduced reaction times, increased yields, and pure products. Schiff bases are the important class of organic compounds due to their flexibility, and structural diversities due to the presence of azomethine group which is helpful for elucidating the mechanism of transformation and rasemination reaction in biological system. This novel compound could also act as valuable ligands for the development of new chemical entities. In the present work, some Schiff bases of Isatin derivatives was synthesized using microwave heating method. Schiff base of Isatin were synthesized by condensation of the keto group of Isatin with different aromatic primary amines. They were characterized by means of spectral data and subsequently subjected to the in vitro antibacterial activities against gram positive and gram negative strains of microbes. It was observed that the compound with electron withdrawing substituents exhibited good antibacterial activities against almost all the micro organisms.
{"title":"Green Chemistry Approach for Efficient Synthesis of Schiff Bases of Isatin Derivatives and Evaluation of Their Antibacterial Activities","authors":"J. Panda, V. Patro, B. Sahoo, Jitendriya Mishra","doi":"10.1155/2013/549502","DOIUrl":"https://doi.org/10.1155/2013/549502","url":null,"abstract":"Microwave-assisted organic synthesis, a green chemistry approach, is nowadays widely used in the drug synthesis. Microwave-assisted synthesis improves both throughput and turnaround time for medicinal chemists by offering the benefits of drastically reduced reaction times, increased yields, and pure products. Schiff bases are the important class of organic compounds due to their flexibility, and structural diversities due to the presence of azomethine group which is helpful for elucidating the mechanism of transformation and rasemination reaction in biological system. This novel compound could also act as valuable ligands for the development of new chemical entities. In the present work, some Schiff bases of Isatin derivatives was synthesized using microwave heating method. Schiff base of Isatin were synthesized by condensation of the keto group of Isatin with different aromatic primary amines. They were characterized by means of spectral data and subsequently subjected to the in vitro antibacterial activities against gram positive and gram negative strains of microbes. It was observed that the compound with electron withdrawing substituents exhibited good antibacterial activities against almost all the micro organisms.","PeriodicalId":16507,"journal":{"name":"Journal of Nanoparticles","volume":"10 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2013-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87189707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CdTe nanocrystals were synthesized by the hot-injection method with a mixture of oleylamine and octadecene as a solvent. The influence of the composition of the solvent and of the injection solution on the shape of CdTe nanoparticles was investigated. Various shapes of CdTe nanocrystals, such as nanodots, nanorods, multipods, and nanowires, could be obtained by changing the reaction conditions. Tuning the reactivity of both the cadmium and the tellurium precursors at the same time was found to be the main reason for the shape control of CdTe nanocrystals in this reaction system. The reactivity of the Cd precursor was controlled by the composition of the solvent, while the activity of the Te precursor could be influenced by using trioctylphosphine and tributylphosphine in the injection solution.
{"title":"Shape Control of CdTe Nanocrystals: Influence of the Solvent Composition and Ligand Effects","authors":"Xiaoping Jin, J. Parisi, J. Kolny-Olesiak","doi":"10.1155/2013/243831","DOIUrl":"https://doi.org/10.1155/2013/243831","url":null,"abstract":"CdTe nanocrystals were synthesized by the hot-injection method with a mixture of oleylamine and octadecene as a solvent. The influence of the composition of the solvent and of the injection solution on the shape of CdTe nanoparticles was investigated. Various shapes of CdTe nanocrystals, such as nanodots, nanorods, multipods, and nanowires, could be obtained by changing the reaction conditions. Tuning the reactivity of both the cadmium and the tellurium precursors at the same time was found to be the main reason for the shape control of CdTe nanocrystals in this reaction system. The reactivity of the Cd precursor was controlled by the composition of the solvent, while the activity of the Te precursor could be influenced by using trioctylphosphine and tributylphosphine in the injection solution.","PeriodicalId":16507,"journal":{"name":"Journal of Nanoparticles","volume":"23 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2013-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80520242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nydeia W. Bolden, V. Rangari, S. Jeelani, S. Boyoglu, S. Singh
In this study, iron oxide (IO) nanoparticles from various precursors have been synthesized using sonochemical method and characterized for their structural variability and toxicity. The iron oxide (IO) precursor solutions were prepared from iron acetate (IA), iron pentacarbonyl (IP), decalin, PEG (poly(ethylene glycol)), EG (ethylene glycol), PVA (poly(vinyl alcohol)), β-cyclodextrin (CD), and distilled water. These precursor solutions were irradiated with high power ultrasound for 3 hours and heat treated as needed. These as-prepared iron oxide nanoparticles were characterized using X-ray diffraction (XRD), Mossbauer spectroscopy, transmission electron microscopy (TEM), and magnetization measurements. XRD results show that all the particles are highly crystalline in nature and the particles sizes measured from TEM are approximately 5–20 nm. The maximum magnetization was observed for IO-IP at approximately 60.17 emu/g and the minimum was approximately 30.56 emu/g for IO-IA. These results confirm that the particles are superparamagnetic (SPM) in nature. Mossbauer spectroscopy verified the magnetic nanoparticles are purely Fe3O4 and particles sizes varied by the nature of the precursor and coatings.
{"title":"Synthesis and Evaluation of Magnetic Nanoparticles for Biomedical Applications","authors":"Nydeia W. Bolden, V. Rangari, S. Jeelani, S. Boyoglu, S. Singh","doi":"10.1155/2013/370812","DOIUrl":"https://doi.org/10.1155/2013/370812","url":null,"abstract":"In this study, iron oxide (IO) nanoparticles from various precursors have been synthesized using sonochemical method and characterized for their structural variability and toxicity. The iron oxide (IO) precursor solutions were prepared from iron acetate (IA), iron pentacarbonyl (IP), decalin, PEG (poly(ethylene glycol)), EG (ethylene glycol), PVA (poly(vinyl alcohol)), β-cyclodextrin (CD), and distilled water. These precursor solutions were irradiated with high power ultrasound for 3 hours and heat treated as needed. These as-prepared iron oxide nanoparticles were characterized using X-ray diffraction (XRD), Mossbauer spectroscopy, transmission electron microscopy (TEM), and magnetization measurements. XRD results show that all the particles are highly crystalline in nature and the particles sizes measured from TEM are approximately 5–20 nm. The maximum magnetization was observed for IO-IP at approximately 60.17 emu/g and the minimum was approximately 30.56 emu/g for IO-IA. These results confirm that the particles are superparamagnetic (SPM) in nature. Mossbauer spectroscopy verified the magnetic nanoparticles are purely Fe3O4 and particles sizes varied by the nature of the precursor and coatings.","PeriodicalId":16507,"journal":{"name":"Journal of Nanoparticles","volume":"76 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2013-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78274411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bombyx mori silk fibroin is a macromolecular biopolymer with remarkable biocompatibility. It was degummed and subjected to a series of treatments, including dissolution and dialysis, to yield an aqueous solution of silk fibroin, which was introduced rapidly into excess acetone to produce crystalline silk fibroin nanoparticles (SFNs), which were conjugated covalently with naringinase using glutaraldehyde as the cross-linking reagent. The SFN naringinases are easily recovered by centrifugation and can be used repeatedly. Naringinase is a bienzyme consisting of α-L-rhamnosidase and flavonoid-β-glucosidase. The enzyme activity and its kinetics were similar to those of the native form, and the optimum reactive temperature for both is 55°C. In our study, centrifugation allowed the separation of enzyme and substrate; after eight cycles the SFN naringinases retained >70% residual activity. The highly efficient processing technology and the use of SFN as a novel vector for a bienzyme have great potential for research and the development of food processing such as the debittering of naringin-containing juices.
家蚕丝素蛋白是一种具有良好生物相容性的高分子生物聚合物。将其脱胶，并进行一系列处理，包括溶解和透析，得到丝素水溶液，将其快速引入过量的丙酮中，产生结晶丝素纳米粒子(SFNs)，并以戊二醛作为交联试剂与柚皮苷酶共价结合。SFN柚皮酶易于离心回收，可重复使用。柚皮苷酶是由α- l -鼠李糖苷酶和类黄酮-β-葡萄糖苷酶组成的双酶。酶的活性和动力学与天然形式相似，两者的最佳反应温度为55℃。在我们的研究中，离心使酶和底物分离;8个循环后，SFN柚皮酶的剩余活性保持在70%以上。该高效加工技术及其作为新型双酶载体的应用，在含柚皮苷果汁的脱臭等食品加工领域具有巨大的研究和开发潜力。
{"title":"Coimmobilization of Naringinases on Silk Fibroin Nanoparticles and Its Application in Food Packaging","authors":"Min-hui Wu, Lin Zhu, Zhenwei Zhou, Yu-Qing Zhang","doi":"10.1155/2013/901401","DOIUrl":"https://doi.org/10.1155/2013/901401","url":null,"abstract":"Bombyx mori silk fibroin is a macromolecular biopolymer with remarkable biocompatibility. It was degummed and subjected to a series of treatments, including dissolution and dialysis, to yield an aqueous solution of silk fibroin, which was introduced rapidly into excess acetone to produce crystalline silk fibroin nanoparticles (SFNs), which were conjugated covalently with naringinase using glutaraldehyde as the cross-linking reagent. The SFN naringinases are easily recovered by centrifugation and can be used repeatedly. Naringinase is a bienzyme consisting of α-L-rhamnosidase and flavonoid-β-glucosidase. The enzyme activity and its kinetics were similar to those of the native form, and the optimum reactive temperature for both is 55°C. In our study, centrifugation allowed the separation of enzyme and substrate; after eight cycles the SFN naringinases retained >70% residual activity. The highly efficient processing technology and the use of SFN as a novel vector for a bienzyme have great potential for research and the development of food processing such as the debittering of naringin-containing juices.","PeriodicalId":16507,"journal":{"name":"Journal of Nanoparticles","volume":"4 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2013-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86527205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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