Pub Date : 2019-01-01Epub Date: 2019-12-12DOI: 10.35248/2157-7439.19.10.539
William Ghann, Tyler Harris, Daiyaan Kabir, Hyeonggon Kang, Mintesinot Jiru, Mohammed M Rahman, Meser M Ali, Jamal Uddin
A simple colorimetric method has been developed for the detection of lead (Pb2+) in water samples using lipoic acid-functionalized gold nanoparticles. The lipoic acid-functionalized gold nanoparticles are induced to aggregate in the presence of the Pb2+ which results in a change in the color of the functionalized gold nanoparticles. The change in color and the amount of Pb2+ producing the change could be monitored via UV-visible spectrophotometry. A good correlation coefficient of 0.9927 was obtained for the calibration curve of the colorimetric method. The method was applied in the determination of Pb2+ in water samples and the results compared to that of measurement carried out with Atomic Absorption Spectroscopy.
{"title":"Lipoic Acid Decorated Gold Nanoparticles and Their Application in the Detection of Lead Ions.","authors":"William Ghann, Tyler Harris, Daiyaan Kabir, Hyeonggon Kang, Mintesinot Jiru, Mohammed M Rahman, Meser M Ali, Jamal Uddin","doi":"10.35248/2157-7439.19.10.539","DOIUrl":"10.35248/2157-7439.19.10.539","url":null,"abstract":"<p><p>A simple colorimetric method has been developed for the detection of lead (Pb<sup>2+</sup>) in water samples using lipoic acid-functionalized gold nanoparticles. The lipoic acid-functionalized gold nanoparticles are induced to aggregate in the presence of the Pb<sup>2+</sup> which results in a change in the color of the functionalized gold nanoparticles. The change in color and the amount of Pb<sup>2+</sup> producing the change could be monitored via UV-visible spectrophotometry. A good correlation coefficient of 0.9927 was obtained for the calibration curve of the colorimetric method. The method was applied in the determination of Pb<sup>2+</sup> in water samples and the results compared to that of measurement carried out with Atomic Absorption Spectroscopy.</p>","PeriodicalId":16532,"journal":{"name":"Journal of Nanomedicine & Nanotechnology","volume":"10 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37587415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.4172/2157-7439.1000522
S. R., M. M, V.N Yogananda Murthy
{"title":"Biosynthesis and Characterization, Antioxidant and Antimicrobial Activities of Selenium Nanoparticles from Ethanol Extract of Bee Propolis","authors":"S. R., M. M, V.N Yogananda Murthy","doi":"10.4172/2157-7439.1000522","DOIUrl":"https://doi.org/10.4172/2157-7439.1000522","url":null,"abstract":"","PeriodicalId":16532,"journal":{"name":"Journal of Nanomedicine & Nanotechnology","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85950333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.35248/2157-7439.19.10.536
Lauréanne Putallaz, P. Bogaard, P. Laub, F. Rebeaud
Protein sensing in a nanofluidic environment dramatically shortens immunoassay time-to-result through the combined action of efficient mass transfer and short diffusion distances. Here, we report on a fully automated point-of-care in vitro diagnostic platform based on disposable capsule containing nanofluidic sensors in which fluorescent immunoassays are performed. Performances of the system were established with three model assays for ferritin, immunoglobulin E (IgE) and pancreatic stone protein (PSP). The described system has the typical high capture efficiency of nano-confined spaces combined with forced-flow, which induce a constant maximal concentration gradient on the sensor. Remarkably, analytes are detected in zeptomole quantities from a drop of blood. Dose-response curves show that high precision and accuracy are achieved in the clinically relevant assay ranges. Moreover, accuracy of the system is excellent agreement with laboratory reference method, as illustrated in a method comparison with total IgE as a model. While several academic proof-of-concepts have already described the possibility to exploit the properties of fluids at the nanoscale to develop immunoassay, the transition of these models to a product fulfilling requirements for use at the point-of-care in terms of operability, affordability, reliability and analytic performances remains a challenging endeavor. This study demonstrates that nanofluidic-based immunoassays can efficiently quantify protein biomarkers in the femto- and picomolar range within ultra-short assay time, high precision and accuracy on a closed, small, easyto- operate platform.
{"title":"Nanofluidics Drives Point-of-care Technology for on the Spot Protein Marker Analysis with Rapid Actionable Results","authors":"Lauréanne Putallaz, P. Bogaard, P. Laub, F. Rebeaud","doi":"10.35248/2157-7439.19.10.536","DOIUrl":"https://doi.org/10.35248/2157-7439.19.10.536","url":null,"abstract":"Protein sensing in a nanofluidic environment dramatically shortens immunoassay time-to-result through the combined action of efficient mass transfer and short diffusion distances. Here, we report on a fully automated point-of-care in vitro diagnostic platform based on disposable capsule containing nanofluidic sensors in which fluorescent immunoassays are performed. Performances of the system were established with three model assays for ferritin, immunoglobulin E (IgE) and pancreatic stone protein (PSP). The described system has the typical high capture efficiency of nano-confined spaces combined with forced-flow, which induce a constant maximal concentration gradient on the sensor. Remarkably, analytes are detected in zeptomole quantities from a drop of blood. Dose-response curves show that high precision and accuracy are achieved in the clinically relevant assay ranges. Moreover, accuracy of the system is excellent agreement with laboratory reference method, as illustrated in a method comparison with total IgE as a model. While several academic proof-of-concepts have already described the possibility to exploit the properties of fluids at the nanoscale to develop immunoassay, the transition of these models to a product fulfilling requirements for use at the point-of-care in terms of operability, affordability, reliability and analytic performances remains a challenging endeavor. This study demonstrates that nanofluidic-based immunoassays can efficiently quantify protein biomarkers in the femto- and picomolar range within ultra-short assay time, high precision and accuracy on a closed, small, easyto- operate platform.","PeriodicalId":16532,"journal":{"name":"Journal of Nanomedicine & Nanotechnology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75579684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.35248/2157-7439.19.10.530
Mousumi Mitra, B. Amit, yopadhyay, G. Datta, N DilipK
Background: The present study aim to investigate on the characterization of green synthesized gold nanoparticles (AuNPs) and to evaluate whether this herbal nanoparticle can increase the efficiency of herb for alteration of hematological indices against acetaminophen induced toxicity in male Wistar rats. Methods: Bark extract of Terminalia arjuna was used for the green synthesis of AuNPs and then characterization of the nanoparticles were done. Then experiment was conducted on 24 healthy male Wistar rats. The animals were divided into four groups, each group having six rats. Group-1: Control; Group- 2: acetaminophen treated (500 mg / kg) for 14 days; Group-3: Co-administration of acetaminophen (500 mg/kg/day) along with Terminalia arjuna bark extract (175 μg/kg/day) for 14 days; Group-4: Co-administration acetaminophen (500 mg/kg/day) along with of green synthesised AuNPs (175 μg/kg/day) for 14 days. Hematological indices were measured using standard hematological techniques. Results: The green synthesized AuNPs were characterized by UV-visible spectroscopy, FESEM, HRTEM, EDX, FTIR, XRD, DLS analysis. UV-visible spectroscopy showed SPR band at 524 nm. FESEM, HRTEM and XRD analyses revealed that green synthesized AuNPs were spherical shaped, crystalline in nature with size ranging between 20 and 40 nm. Hematological analysis revealed that there was significant decrease in Red Blood Cells (RBCs), Hemoglobin (HB), Hematocrit (HCT)%, Lymphocyte percentage and Platelet Distribution Width (PDW)%, with acetaminophen treatment but White Blood Cells(WBCs), Red blood cell Distribution Width (RDW)% and Platelets (PLTs) significantly increases with acetaminophen administration. Then after co-administration with green synthesized AuNPs along with acetaminophen showed effective significant recovery in the hematological alterations. Conclusions: Overall the results highlighted the promising effect of green synthesized AuNPs against acetaminophen induced hematological alterations in male Wistar rats.
{"title":"Protective Role of Green Synthesized Gold Nanoparticles Using Terminalia arjuna against Acetaminophen Induced Hematological Alterations in Male Wistar Rats","authors":"Mousumi Mitra, B. Amit, yopadhyay, G. Datta, N DilipK","doi":"10.35248/2157-7439.19.10.530","DOIUrl":"https://doi.org/10.35248/2157-7439.19.10.530","url":null,"abstract":"Background: The present study aim to investigate on the characterization of green synthesized gold nanoparticles (AuNPs) and to evaluate whether this herbal nanoparticle can increase the efficiency of herb for alteration of hematological indices against acetaminophen induced toxicity in male Wistar rats. Methods: Bark extract of Terminalia arjuna was used for the green synthesis of AuNPs and then characterization of the nanoparticles were done. Then experiment was conducted on 24 healthy male Wistar rats. The animals were divided into four groups, each group having six rats. Group-1: Control; Group- 2: acetaminophen treated (500 mg / kg) for 14 days; Group-3: Co-administration of acetaminophen (500 mg/kg/day) along with Terminalia arjuna bark extract (175 μg/kg/day) for 14 days; Group-4: Co-administration acetaminophen (500 mg/kg/day) along with of green synthesised AuNPs (175 μg/kg/day) for 14 days. Hematological indices were measured using standard hematological techniques. Results: The green synthesized AuNPs were characterized by UV-visible spectroscopy, FESEM, HRTEM, EDX, FTIR, XRD, DLS analysis. UV-visible spectroscopy showed SPR band at 524 nm. FESEM, HRTEM and XRD analyses revealed that green synthesized AuNPs were spherical shaped, crystalline in nature with size ranging between 20 and 40 nm. Hematological analysis revealed that there was significant decrease in Red Blood Cells (RBCs), Hemoglobin (HB), Hematocrit (HCT)%, Lymphocyte percentage and Platelet Distribution Width (PDW)%, with acetaminophen treatment but White Blood Cells(WBCs), Red blood cell Distribution Width (RDW)% and Platelets (PLTs) significantly increases with acetaminophen administration. Then after co-administration with green synthesized AuNPs along with acetaminophen showed effective significant recovery in the hematological alterations. Conclusions: Overall the results highlighted the promising effect of green synthesized AuNPs against acetaminophen induced hematological alterations in male Wistar rats.","PeriodicalId":16532,"journal":{"name":"Journal of Nanomedicine & Nanotechnology","volume":"48 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79458379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-17DOI: 10.4172/2157-7439-c7-084
pMinoru Tayap
{"title":"Design of nanorobotics based on flexible FePd nanohelix for cancer treatment","authors":"pMinoru Tayap","doi":"10.4172/2157-7439-c7-084","DOIUrl":"https://doi.org/10.4172/2157-7439-c7-084","url":null,"abstract":"","PeriodicalId":16532,"journal":{"name":"Journal of Nanomedicine & Nanotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74254234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-27DOI: 10.4172/2157-7439-C4-076
R. Bushra, J. Sakdapipanich
{"title":"Fabrication of ternary nanocomposite material and its potential applications","authors":"R. Bushra, J. Sakdapipanich","doi":"10.4172/2157-7439-C4-076","DOIUrl":"https://doi.org/10.4172/2157-7439-C4-076","url":null,"abstract":"","PeriodicalId":16532,"journal":{"name":"Journal of Nanomedicine & Nanotechnology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80073630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-04DOI: 10.4172/2157-7439.1000504
Uzma Azam
Major aim of this research study is to comprehend the interaction of soft nanoparticle-based vesicles with model lung surfactant monolayer. Vertebrate lung surfactant plays a pivotal role in gaseous exchange plus breathing. As pulmonary lung surfactant is the one which directly comes in contact to inhaled air so it also acts as defensive agent against air-borne pollutants (any foreign particle). In this enquiry, several different types of lipids namely 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, 1,2-Dimitrystoyl-sn-glycero-3-phosphorylcholine, 1-palmitoyl-2-oleoyl-sn-glyceero-3-phosphocholine are used as lipid monolayer and vesicles in different combinations while using Langmuir-Blodgett trough. The different lipids are treated with chloroform and buffer solution to make vesicles and then extruded. Nanoparticles present in vesicles interact with lipid monolayer when processed in Langmuir-Blodgett trough and mimics the behavior of natural pulmonary lung surfactant system. In Quartz Crystal with gold mounted on it as thin layer, different combinations of lipid bilayer and vesicles are used from the same list of lipids. The results obtained from this investigation are quite informative in depicting the interaction of soft nanoparticle based vesicles with lung surfactant system, also this work would pave the way to explore still more on interaction of soft nanoparticles based vesicles with lung surfactant, as very little work is done on soft nanoparticles. This novelty of this work is to plan to develop some alternatives of lung surfactant because we cannot perform the experiment directly in living souls as some of these has lethal effects.
{"title":"Investigation of Interaction of Soft Nanoparticles based Vesicles with Lung Surfactant via Langmuir-Blodgett trough and Quartz Crystal Microbalance Study","authors":"Uzma Azam","doi":"10.4172/2157-7439.1000504","DOIUrl":"https://doi.org/10.4172/2157-7439.1000504","url":null,"abstract":"Major aim of this research study is to comprehend the interaction of soft nanoparticle-based vesicles with model lung surfactant monolayer. Vertebrate lung surfactant plays a pivotal role in gaseous exchange plus breathing. As pulmonary lung surfactant is the one which directly comes in contact to inhaled air so it also acts as defensive agent against air-borne pollutants (any foreign particle). In this enquiry, several different types of lipids namely 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, 1,2-Dimitrystoyl-sn-glycero-3-phosphorylcholine, 1-palmitoyl-2-oleoyl-sn-glyceero-3-phosphocholine are used as lipid monolayer and vesicles in different combinations while using Langmuir-Blodgett trough. The different lipids are treated with chloroform and buffer solution to make vesicles and then extruded. Nanoparticles present in vesicles interact with lipid monolayer when processed in Langmuir-Blodgett trough and mimics the behavior of natural pulmonary lung surfactant system. In Quartz Crystal with gold mounted on it as thin layer, different combinations of lipid bilayer and vesicles are used from the same list of lipids. The results obtained from this investigation are quite informative in depicting the interaction of soft nanoparticle based vesicles with lung surfactant system, also this work would pave the way to explore still more on interaction of soft nanoparticles based vesicles with lung surfactant, as very little work is done on soft nanoparticles. This novelty of this work is to plan to develop some alternatives of lung surfactant because we cannot perform the experiment directly in living souls as some of these has lethal effects.","PeriodicalId":16532,"journal":{"name":"Journal of Nanomedicine & Nanotechnology","volume":"33 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2018-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90039051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-04DOI: 10.4172/2157-7439.1000502
A. Rajak
In modern days, technology is growing rapidly. Technology is the benefit of science by promoting the benefit of society. Science is critical to future development of nanotechnology. Nanotechnology is the developments for future. Nanoparticles can cause chronic health effects. Viruses and DNA are examples of natural objects on the nanoscale. Nanoscience is study of manipulation of materials at atomic, molecular and macromolecular scales. Nanotechnology also has the potential opportunities to improves create new and better product. Application of nanomaterials to detect, prevent and remove pollution. Nanotechnologies are design, production and applications of medicine, diagnostic, therapy, sequencing. Nanotechnology may be able to advance environmental protection. Nanotechnology is potential to contribute to reduction in energy. Nanotechnology represents an entire scientific and engineering field. This is rapidly growing and large future market.
{"title":"Nanotechnology and Its Application","authors":"A. Rajak","doi":"10.4172/2157-7439.1000502","DOIUrl":"https://doi.org/10.4172/2157-7439.1000502","url":null,"abstract":"In modern days, technology is growing rapidly. Technology is the benefit of science by promoting the benefit of society. Science is critical to future development of nanotechnology. Nanotechnology is the developments for future. Nanoparticles can cause chronic health effects. Viruses and DNA are examples of natural objects on the nanoscale. Nanoscience is study of manipulation of materials at atomic, molecular and macromolecular scales. Nanotechnology also has the potential opportunities to improves create new and better product. Application of nanomaterials to detect, prevent and remove pollution. Nanotechnologies are design, production and applications of medicine, diagnostic, therapy, sequencing. Nanotechnology may be able to advance environmental protection. Nanotechnology is potential to contribute to reduction in energy. Nanotechnology represents an entire scientific and engineering field. This is rapidly growing and large future market.","PeriodicalId":16532,"journal":{"name":"Journal of Nanomedicine & Nanotechnology","volume":"16 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2018-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87873748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-25DOI: 10.4172/2157-7439.1000505
Hala Elia
As a crucial element in construction, tunnels, roads, pavements, and more, concrete has become one of the most important materials in the world. At the same time, air pollution, particularly in crowded cities, is increasing, mainly due to industrial activity and transportation. Therefore, one possible approach to fighting pollution is the use of “smart” construction materials, particularly the incorporation of photocatalytically active nano- and micro structures into concrete. Incorporating titanium dioxide (TiO2) in roads and pavements could degrade and, as a result, reduce various pollutants under ultraviolet radiation. The TiO2-infused concrete would also maintain its optical characteristics for far longer than traditional concrete mix. This research presents an evaluation of nano- and micro-TiO2-incorporated concretion degradation of organic molecules, as assessed by the concrete’s ability to degrade Rhodamine B dye. We tested concrete blocks with different concentrations of nano- and micro-TiO2 in their structure, exposing them to sunlight for various periods of time (24,48,72, and 96 hrs). The percentage of nano- and micro-TiO2 used in this research was 3,6,9,12, and 15% of the cement composition. The results showed good degradation of the Rhodamine B dye by both nano- and micro-TiO2, demonstrating the potential of this approach towards smarted construction materials for environmental applications.
{"title":"Using Nano- and Micro-Titanium Dioxide (TiO2) in Concrete to Reduce Air Pollution","authors":"Hala Elia","doi":"10.4172/2157-7439.1000505","DOIUrl":"https://doi.org/10.4172/2157-7439.1000505","url":null,"abstract":"As a crucial element in construction, tunnels, roads, pavements, and more, concrete has become one of the most important materials in the world. At the same time, air pollution, particularly in crowded cities, is increasing, mainly due to industrial activity and transportation. Therefore, one possible approach to fighting pollution is the use of “smart” construction materials, particularly the incorporation of photocatalytically active nano- and micro structures into concrete. Incorporating titanium dioxide (TiO2) in roads and pavements could degrade and, as a result, reduce various pollutants under ultraviolet radiation. The TiO2-infused concrete would also maintain its optical characteristics for far longer than traditional concrete mix. This research presents an evaluation of nano- and micro-TiO2-incorporated concretion degradation of organic molecules, as assessed by the concrete’s ability to degrade Rhodamine B dye. We tested concrete blocks with different concentrations of nano- and micro-TiO2 in their structure, exposing them to sunlight for various periods of time (24,48,72, and 96 hrs). The percentage of nano- and micro-TiO2 used in this research was 3,6,9,12, and 15% of the cement composition. The results showed good degradation of the Rhodamine B dye by both nano- and micro-TiO2, demonstrating the potential of this approach towards smarted construction materials for environmental applications.","PeriodicalId":16532,"journal":{"name":"Journal of Nanomedicine & Nanotechnology","volume":"25 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82234243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号