Journal of oral biology and craniofacial research最新文献

{"title":"A novel histopathological subclassification of oral submucous fibrosis based on epithelial keratinization: A proof-of-concept pilot observation","authors":"Gargi S. Sarode,&nbsp;Sachin C. Sarode","doi":"10.1016/j.jobcr.2025.11.012","DOIUrl":"10.1016/j.jobcr.2025.11.012","url":null,"abstract":"<div><div>Oral submucous fibrosis (OSMF) is a chronic, progressive oral potentially malignant disorder strongly associated with areca nut consumption. Despite extensive research, a reliable and clinically applicable biomarker for predicting malignant transformation remains unavailable. The present pilot investigation introduces a novel concept by classifying OSMF into two histopathological variants—hyperkeratinized and non-hyperkeratinized—based on epithelial keratinization patterns, a distinction not previously described in the literature. Archival biopsy samples of 51 histologically confirmed OSMF cases from the buccal mucosa were analyzed for keratinization type and epithelial dysplasia. Additionally, 37 cases of oral squamous cell carcinoma arising in the background of OSMF (OSCC-OSMF) were examined for keratinization at surgical margins. Among OSMF cases, 19.6 % exhibited hyperkeratinization, all showing high-risk epithelial dysplasia, whereas 90.2 % of normally keratinized cases demonstrated low-risk dysplasia. In OSCC-OSMF specimens, 60 % of surgical margins revealed hyperkeratinization. These findings suggest that epithelial hyperkeratinization may represent an early histological alteration associated with malignant transformation rather than a protective epithelial response. Although limited by small sample size, this study provides proof-of-concept for a novel histopathological subclassification of OSMF, warranting validation through larger, multicentre longitudinal and molecular correlation studies.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 129-131"},"PeriodicalIF":0.0,"publicationDate":"2025-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145621088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility and odontogenic potential of an indigenous apitherapeutic pulp capping agent: A comparative in vitro analysis using hDPSCs","authors":"Ketaki Turbatmath ,&nbsp;S. Delphine Priscilla Antony ,&nbsp;Raghunandhakumar Subramanian","doi":"10.1016/j.jobcr.2025.11.008","DOIUrl":"10.1016/j.jobcr.2025.11.008","url":null,"abstract":"<div><h3>Introduction</h3><div>This study aimed to evaluate the cytocompatibility and odonto-inductive potential of an indigenously developed apitherapeutic pulp capping agent Api-Therapeutic Pulp Guard (ATPG) by assessing cell viability and dentinogenic gene expression using human dental pulp stem cells (hDPSCs)</div></div><div><h3>Materials and methods</h3><div>hDPSCs were exposed to eluates of ATPG and compared with calcium hydroxide (RC-Cal) and control. MTT assay was used to assess cell viability, LIVE/DEAD staining was performed using calcein-AM and ethidium homodimer, and quantitative real-time PCR (qPCR) evaluated DSPP gene expression. This comprehensive approach provided complementary insights into cell metabolic activity, membrane integrity, and odontogenic potential of the test materials.</div></div><div><h3>Results</h3><div>ATPG exhibited high cell viability (96.52 %), significantly greater than calcium hydroxide (84.25 %) (<em>p</em> &lt; 0.01). LIVE/DEAD assay confirmed better membrane integrity with a predominance of green (live) stained cells. qPCR showed a 3.6-fold upregulation of DSPP gene expression in ATPG-treated cells, suggesting strong odontogenic differentiation.</div></div><div><h3>Conclusion</h3><div>The ATPG formulation demonstrated superior biocompatibility and odontogenic potential compared to conventional calcium hydroxide, underscoring its promise for translation in regenerative endodontic therapy.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 113-119"},"PeriodicalIF":0.0,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145621089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SFXN1 expression analysis in oral squamous cell carcinoma and its association with the PI3K-AKT-mTOR pathway and immune cell infiltration","authors":"Prabhu Manickam Natarajan ,&nbsp;Manoj Kumar Karuppan Perumal ,&nbsp;Sudhir Rama Varma ,&nbsp;Sam Thomas Kuriadom ,&nbsp;Ruba Odeh ,&nbsp;Remya Rajan Renuka","doi":"10.1016/j.jobcr.2025.11.010","DOIUrl":"10.1016/j.jobcr.2025.11.010","url":null,"abstract":"<div><h3>Background</h3><div>Oral squamous cell carcinoma (OSCC) is a predominant malignancy characterized by aggressive progression and poor prognosis. This study investigated the role of SFXN1 (Sideroflexin 1), a mitochondrial serine transporter, in OSCC using integrated bioinformatic and experimental approaches.</div></div><div><h3>Objective</h3><div>To analyze the expression, clinical relevance, and functional associations of SFXN1 in OSCC through comprehensive bioinformatic and in vitro investigations.</div></div><div><h3>Methods</h3><div>Transcriptomic data from TCGA pan-cancer cohorts were analyzed to evaluate SFXN1 expression patterns. The expression levels in OSCC were validated using KB OC cell lines, with clinical correlations assessed for tumor grade, nodal status, and patient survival. Immune infiltration associations and protein‒protein interaction networks were constructed, followed by pathway enrichment analyses. Experimental validation was performed via in vitro assays.</div></div><div><h3>Results</h3><div>The results revealed that SFXN1 was significantly overexpressed in head and neck squamous cell carcinoma and markedly upregulated in KB cells compared with controls. SFXN1 expression was associated with tumor grade and nodal metastasis, although no significant stage-specific differences were observed. Survival analysis revealed no statistically significant association with overall survival. Immune infiltration analysis indicated modest but significant correlations between SFXN1 and immune cell populations, particularly CD4⁺ T cells. Protein network analysis identified hub genes, including AKT1, BCL2, MTOR, and CASP3. Pathway enrichment implicated SFXN1 is involved in the PI3K-AKT-mTOR and p53 signaling pathways.</div></div><div><h3>Conclusion</h3><div>These findings highlight the involvement of SFXN1 in cancer-related pathways and its potential role in OSCC, suggesting potential therapeutic targeting opportunities that need further investigation.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 120-128"},"PeriodicalIF":0.0,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145621090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge, attitudes, and practices of dentists toward geriatric health and nutrition: A pan-India cross-sectional questionnaire-based study","authors":"B.T. Pradeep Raja ,&nbsp;P.S. Manoharan ,&nbsp;E. Rajkumar ,&nbsp;G. Ezhumalai","doi":"10.1016/j.jobcr.2025.11.009","DOIUrl":"10.1016/j.jobcr.2025.11.009","url":null,"abstract":"<div><h3>Background</h3><div>India's rapidly growing geriatric population faces significant oral health and nutritional challenges. Although prosthodontic care can improve dietary intake, dental practitioners' awareness and integration of geriatric nutrition into routine practice remain unclear. Limited data exist on how Indian dentists address these needs in clinical and educational settings.</div></div><div><h3>Objective</h3><div>This study evaluated the knowledge, attitudes, and practices (KAP) of dental practitioners across India regarding geriatric health and nutrition and identified gaps to inform education, policy, and clinical interventions.</div></div><div><h3>Methods</h3><div>A cross-sectional survey was conducted among 462 dental practitioners providing prosthodontic care across 21 states. A validated 28-item questionnaire, including three open-ended questions, assessed KAP. Data were analyzed using descriptives, one-way ANOVA, and Post Hoc Bonferroni tests (p ≤ 0.05).</div></div><div><h3>Results</h3><div>Respondents were aware of nutritional assessment methods (72.1 %) and recognized the importance of dietary counselling (97.8 %). Attitudes were highly positive, with 98.1 % supporting inclusion of geriatric nutrition in curriculum. However, only 52 % routinely assessed nutrition, and 63 % provided diet counselling. Prosthodontists had significantly higher knowledge (77.87 %) and practice scores (65.64 %), while institutional practitioners showed the most positive attitudes (74.12 %). Notably, 70 % reported insufficient training during dental education.</div></div><div><h3>Conclusion</h3><div>This first pan-India study highlights a substantial gap between knowledge and practice in geriatric nutrition. Despite positive attitudes, routine implementation is limited. Structured curriculum reforms, continuing education, and chairside intervention models are urgently needed to enhance oral and systemic health outcomes in the elderly.</div></div><div><h3>Novelty</h3><div>This nationwide assessment uniquely identifies educational and practice gaps, offering <strong>evidence to</strong> guide policy and clinical interventions to improve geriatric dental care in India.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 106-112"},"PeriodicalIF":0.0,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145577529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of postoperative pain in patients using 8.25 % sodium hypochlorite compared with 5.25 % sodium hypochlorite using sonic and ultrasonic activation methods after single visit root canal treatment: an in-vivo study","authors":"Kabir Bulchandani,&nbsp;Shahina Parvez,&nbsp;Lalit Kumar Likhyani,&nbsp;Manoj Kumar Agarwal,&nbsp;Rohit Kumar Khatri,&nbsp;Deepak Goel","doi":"10.1016/j.jobcr.2025.11.004","DOIUrl":"10.1016/j.jobcr.2025.11.004","url":null,"abstract":"<div><h3>Introduction</h3><div>The aim of this study was to evaluate the postoperative pain in patients using 8.25 % sodium hypochlorite compared to 5.25 % Sodium Hypochlorite concentration without activation and using sonic and ultrasonic activation methods after single visit root canal treatment.</div></div><div><h3>Methods</h3><div>In this double blind randomized trial, a total of 120 participants mandibular premolar with symptomatic irreversible pulpitis were included and randomly divided into 6 groups based on the different concentration of sodium hypochlorite and activation methods (sonic &amp; ultrasonic activation) i.e. Group 1: 5.25 % NaOCl with no activation method, Group 2: 8.25 % NaOCl with no activation method, Group 3: 5.25 % NaOCl with sonic activation, Group 4: 5.25 % NaOCl with Ultrasonic activation, Group 5: 8.25 % NaOCl with sonic activation, Group 6: 8.25 % NaOCl with ultrasonic activation. The presence of postoperative pain was assessed 24, 48, and 72 h after treatment and recorded using the visual analogue scale (VAS).</div></div><div><h3>Results</h3><div>Intragroup postoperative pain in all six groups reduced after 24 h, 48 h and 72 h, showing statistically significant differences. At all-time intervals, postoperative pain was higher in the groups that used ultrasonic stimulation.</div></div><div><h3>Conclusion</h3><div>At all-time intervals, there was no significant difference in the presence of postoperative pain between the 8.25 % NaOCl and 5.25 % NaOCl irrigation solutions with no activation method and sonic activation compared to ultrasonic activation.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 101-105"},"PeriodicalIF":0.0,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145527677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic synthesis of copper oxide nanoparticles using Alpinia calcarata extract promotes osteoblasts differentiation: An In vitro study","authors":"Rathika Ravi ,&nbsp;Deepavalli Arumuganainar","doi":"10.1016/j.jobcr.2025.11.007","DOIUrl":"10.1016/j.jobcr.2025.11.007","url":null,"abstract":"<div><h3>Background</h3><div>Copper-based biomaterials are increasingly investigated for bone tissue engineering due to their osteogenic and angiogenic properties. Green synthesis using plant extracts offers an eco-friendly route for producing biocompatible nanoparticles. This study aimed to synthesize copper oxide nanoparticles (CuO NPs) using <em>Alpinia calcarata</em> leaf extract and evaluate their cytocompatibility and osteogenic potential in vitro.</div></div><div><h3>Methods</h3><div>CuO NPs were synthesized by reacting copper nitrate trihydrate with A. calcarata leaf extract under alkaline conditions, followed by calcination. The nanoparticles were characterized using SEM, TEM, XRD, and FTIR to confirm morphology, crystallinity, and functional group interactions. C3H10T1/2 murine mesenchymal stem cells were used to assess cytocompatibility (MTT assay, FDA staining), osteogenic differentiation (Alizarin Red S staining and quantification), and gene expression (qRT-PCR for Runx2, Col-I, and ALP).</div></div><div><h3>Results</h3><div>SEM and TEM revealed aggregated, spherical nanoparticles of &lt;100 nm, while XRD confirmed crystalline monoclinic CuO and FTIR indicated phytochemical capping. MTT and FDA assays showed CuO NPs were cytocompatible up to 50 μg/mL, with dose-dependent cytotoxicity observed at higher concentrations. Under osteogenic conditions, cells treated with 50 μg/mL CuO NPs displayed significantly enhanced mineral deposition compared to controls. Gene expression analysis demonstrated upregulation of Runx2, Col-I, and ALP, confirming promotion of osteogenic differentiation.</div></div><div><h3>Conclusion</h3><div>Biogenically synthesized CuO NPs using A. calcarata extract are structurally pure, biocompatible at defined concentrations, and capable of enhancing osteoblast differentiation by stimulating matrix mineralization and osteogenic gene expression. These findings position A. calcarata–mediated CuO NPs as sustainable, multifunctional nanomaterials with promising applications in bone tissue engineering.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 66-72"},"PeriodicalIF":0.0,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145527673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional recovery of demineralized dentin using a glutamic acid-modified electrospun scaffold: A multimodal in vitro characterization","authors":"Aruna Krishnan ,&nbsp;Sandhya Raghu ,&nbsp;Govindaraj Perumal ,&nbsp;Jayalakshmi Somasundaram ,&nbsp;Nishitha Arun","doi":"10.1016/j.jobcr.2025.11.005","DOIUrl":"10.1016/j.jobcr.2025.11.005","url":null,"abstract":"<div><h3>Background</h3><div>Dentin remineralization poses a significant challenge in restorative dentistry due to the complex structure and mineral-organic composition of dentin. Electrospun nanofibrous scaffolds present a biomimetic platform to facilitate mineral deposition. Functionalization of the scaffold, achieved through the incorporation of biomolecules like glutamic acid, which mimics non-collagenous proteins, may improve remineralization.</div></div><div><h3>Objective</h3><div>To assess the remineralization potential of a glutamic acid-loaded polycaprolactone/nanohydroxyapatite (PCL/nHA/Glu) scaffold on acid-demineralized dentin, through surface and mechanical characterization.</div></div><div><h3>Methods</h3><div>Nanofibrous scaffolds of PCL/nHA and PCL/nHA/Glu were fabricated and applied to demineralized human dentin discs. The samples were immersed in simulated body fluid for 7, 14, and 28 days. Changes in morphology and composition were analyzed using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). Surface topography was assessed by atomic force microscopy (AFM), and mechanical properties were evaluated via nanoindentation, with statistical analysis conducted using one-way ANOVA.</div></div><div><h3>Results</h3><div>SEM imaging showed progressive mineral deposition in both scaffold groups, with the PCL/nHA/Glu group demonstrating organized crystallite formation and almost complete tubule occlusion by Day 28. EDS indicated earlier and higher Ca and P incorporation in the PCL/nHA/Glu group. AFM showed significant reductions in surface roughness later, while nanoindentation revealed increased elastic modulus and hardness in the PCL/nHA/Glu group by Day 14, indicating mechanical recovery.</div></div><div><h3>Conclusion</h3><div>Glutamic acid-functionalized scaffolds significantly enhanced the remineralization of demineralized dentin, promoting organized mineral deposition and restoration of mechanical properties. These findings support using amino acid-modified scaffolds in dentin tissue engineering.</div></div><div><h3>Clinical significance</h3><div>Functionalization with amino acids is crucial for mimicking the activity of non-collagenous proteins, transforming passive ion delivery systems into bioactive platforms for dentin regeneration. These scaffolds could serve as alternatives to traditional materials in restorative dentistry, aiding in the treatment of dental caries, dentin hypersensitivity, and enhancing pulp vitality and regeneration.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 73-82"},"PeriodicalIF":0.0,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145527675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a machine learning model to predict mandibular third molar impaction and associated complications: A retrospective observational study","authors":"Nancy Jidiya ,&nbsp;Parth Rathi ,&nbsp;Sravanthi Ennala ,&nbsp;Anulatha Manne ,&nbsp;C.P. Muhammed Faiz ,&nbsp;T.H. Farzhana ,&nbsp;Shikhar Daniel","doi":"10.1016/j.jobcr.2025.10.026","DOIUrl":"10.1016/j.jobcr.2025.10.026","url":null,"abstract":"<div><h3>Background</h3><div>Impacted mandibular third molars are common worldwide and may cause complications such as pericoronitis, cysts, and second molar caries. Conventional prediction methods rely on subjective radiographic interpretations and clinical judgment, which can vary among practitioners. Recent advances in machine learning (ML) provide opportunities to develop objective, data-driven models for clinical decision-making in third molar management.</div></div><div><h3>Aim</h3><div>To develop and validate a machine learning–based model for predicting mandibular third molar impaction and associated complications using demographic and radiographic variables.</div></div><div><h3>Methods</h3><div>This retrospective observational study evaluated 220 panoramic radiographs of patients aged 16–40 years. Collected variables included age, sex, tooth angulation, Pell and Gregory classification, depth of impaction, root development, ramus relationship, and proximity to the mandibular canal. Logistic regression, random forest, and XGBoost were trained on 70 % of the dataset and validated on 30 %. Model performance was assessed using AUC-ROC, accuracy, sensitivity, specificity, calibration, and Cohen's Kappa for agreement with expert judgment.</div></div><div><h3>Results</h3><div>Impaction prevalence was 67.3 %. Significant predictors included mesioangular angulation, Pell and Gregory Class II, and incomplete root development (p &lt; 0.001). XGBoost outperformed other models, achieving an AUC-ROC of 0.92, accuracy of 90.5 %, and Kappa of 0.82. Pericoronitis (26 %) and distal second molar caries (18 %) were the most common complications.</div></div><div><h3>Conclusion</h3><div>XGBoost demonstrated high predictive accuracy for mandibular third molar impaction and complications. As a probability-based decision-support tool, it can provide individualized risk estimates or binary classifications to assist clinicians in counseling, surveillance, and surgical decision-making.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 83-92"},"PeriodicalIF":0.0,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145527676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of chlorogenic acids and melanoidin interactions with salivary proteins and their effect on tooth discoloration","authors":"Trianna Wahyu Utami ,&nbsp;Muhammad Raka Aqila Zaky ,&nbsp;Margareta Rinastiti ,&nbsp;Diatri Nari Ratih ,&nbsp;Sri Budi Barunawati ,&nbsp;Nunuk Purwanti ,&nbsp;Yosi Bayu Murti","doi":"10.1016/j.jobcr.2025.11.006","DOIUrl":"10.1016/j.jobcr.2025.11.006","url":null,"abstract":"<div><h3>Background</h3><div>Tooth discoloration is a multifactorial process that is significantly influenced by the interaction between dietary polyphenols and salivary proteins. This study investigates coffee-induced discoloration using in vitro staining models and in silico simulations.</div></div><div><h3>Materials and methods</h3><div>For 14 days, human premolar teeth were soaked in coffee solution (27 g/450 mL) with daily replacement. Lab and ΔE measurements were used to quantify color changes before and after immersion using ultraviolet–visible spectroscopy. Melanoidin compounds docked with salivary proteins (statherin, histatin, and proline-rich protein [PRP]). Hex 8.0 was used for molecular docking, whereas Discovery Studio Visualizer and UCSF Chimera were used for molecular dynamics analysis.</div></div><div><h3>Results</h3><div>Molecular docking revealed the strong binding affinity of chlorogenic acid with PRP (−251.66 kJ/mol), histatin (−245.4 kJ/mol), and statherin (−240.5 kJ/mol), stabilized by hydrogen bonds with glycine/arginine/tyrosine residues. The molecular dynamics simulations confirmed complex stability with statherin (RMSD 4.2–4.6 Å) and histatin (RMSD 4.4–4.6 Å). Chromameter analysis showed significant color changes after coffee exposure (ΔEab = 12.286 ± 3.645), with a decrease in lightness (L: 83.569 → 71.873) and an increase in redness (a*: 1.375 → 2.992) and yellowness (b*: 15.848 → 18.585). Paired samples correlation analysis revealed statistically significant changes in the a* and b* values (p &lt; 0.001), while the L* value showed no significant change (p = 0.257).</div></div><div><h3>Conclusion</h3><div>Chlorogenic acid drives discoloration through stable protein interactions and measurable color shifts, suggesting that targeted inhibition of these molecular pathways could prevent coffee-induced discoloration.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 93-100"},"PeriodicalIF":0.0,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145527620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lateral stress distribution analysis on hafnium metal bar for implant superstructure- A finite element analysis","authors":"Budati Meghna ,&nbsp;Vaishnavi Rajaraman ,&nbsp;Padma Ariga ,&nbsp;Saravanan Sekaran ,&nbsp;K Lokesh sai","doi":"10.1016/j.jobcr.2025.11.003","DOIUrl":"10.1016/j.jobcr.2025.11.003","url":null,"abstract":"<div><h3>Background</h3><div>The mechanical behavior of implant-supported prostheses is strongly influenced by the material properties of the bar framework, particularly under lateral masticatory loading. While titanium and cobalt–chromium are established materials, alternatives such as polyetheretherketone (PEEK) and hafnium may offer distinct biomechanical advantages. The aim of this study was to compare the lateral stress distribution in implant-supported prostheses fabricated with four different bar materials using finite element analysis.</div></div><div><h3>Method</h3><div>ology: A three-dimensional finite element analysis (FEA) was conducted on a mandibular edentulous model with four endosseous implants arranged in an All-on-4 configuration. Four groups were evaluated based on bar material: titanium (Ti), cobalt–chromium (CoCr), polyetheretherketone (PEEK), and hafnium (Hf). A total of four simulation models were created, each subjected to a 100 N anterior and 300 N posterior lateral load. Von Mises stresses were analyzed in the bar, abutments, screws, and peri-implant bone.</div></div><div><h3>Results</h3><div>PEEK recorded the lowest stress in the bar (∼25 MPa) and peri-implant bone but induced elevated stresses in the abutment (348 MPa) and screw (288 MPa). CoCr showed the highest stress within the bar (∼114 MPa) with lower values in other components. Titanium and hafnium presented a balanced stress profile, with no region exceeding 300 MPa. Hafnium closely mirrored titanium's biomechanical response, demonstrating efficient load distribution without critical stress concentrations.</div></div><div><h3>Conclusion</h3><div>Bar material significantly affects lateral stress distribution in implant-supported prostheses. Hafnium displayed biomechanical behavior comparable to titanium, indicating its promise as a potential alternative framework material. Further in vitro and clinical validation is warranted.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 52-58"},"PeriodicalIF":0.0,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145527672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}