Journal of oral biology and craniofacial research最新文献

Background

Salivary Hemoglobin (SH) has emerged as the mainstay non-invasive and a practicable screening method for Chronic Periodontitis. Current research aims to comprehensively assess the diagnostic value of Salivary Hb (SH) in comparison with Salivary IL-6 (SIL-6) and levels of Salivary lactate dehydrogenase enzyme (SLDH) amongst Type II Diabetes subjects having Chronic Periodontitis (CP) and associated tooth loss.

Materials and methods

In this cross-sectional comparative investigation, 240 individuals with at least 15 remaining teeth, ranging in age from 30 to 70, were chosen and Group I controls were defined as follows: healthy (HbA1c levels ≤6.4 %) with no CP; Group II included chronic periodontitis and non-T2DM (HbA1c ≤ 6.4 %); Group III included T2DM (HbA1c ≥ 6.5 %) and CP; and Group IV included T2DM (HbA1c ≥ 6.5 %) with periodontitis-related tooth loss. ELISA colorimetric assay was used to quantify the results using the unstimulated whole saliva of fasting participants. Tukey's post hoc test was used for statistical analysis following Analysis of Variance (ANOVA), and Sensitivity and Specificity were computed following the determination of the correlation coefficient.

Results

One-way ANOVA comparing Biomarker levels across the four groups revealed a statistically significant difference (F = 68.013) (p = 0.0001). Tukey's multiple post hoc yielded a significant difference between groups with least mean average biomarker levels observed among the controls (Group1) and maximum with group IV. Diagnostic Accuracy to discriminate between CP in T2DM & Controls with SH surpassed that of SIL-6 & SLDH, Receiver operating characteristic (ROC) curve depicted an overall sensitivity of 67.62 %, specificity of 80 % and accuracy of 74 % in T2DM subjects with tooth loss for the identification and assessment of CP.

Conclusion

Estimates of Salivary Hemoglobin can assume an important role in comparison to SIL-6 & SLDH in determining the degree of periodontitis, including tooth loss, and identifying elevated glycemic levels. Advanced detection and monitoring can be ensured by routine use in dental offices and general practice.

Background

Knowledge of the anatomy and morphology of root canal orifices and variations are vital elements affecting treatment outcomes.

Aim

The objective of this study was to evaluate variations in the number of root canal orifices and their patterns in primary teeth, as identified by both the naked eye and under magnifying loupes.

Materials and methods

Total of 173 primary teeth was scheduled for pulpectomy over a period of 18 months. Two examiners assessed the number and pattern of the root canal orifices. After access cavity preparation, the operator recorded the number of root canal orifices with naked eye, and examiner recorded the same using magnifying loupes (3.5×). After cleaning and shaping, the same protocol was used. Collected data were statistically analyzed using SPSS version 23.0 and compared using a paired t-test.

Results

The overall variation in the in the identification of root canal orifices between the naked eye and magnifying loupes (3.005 ± 0.971) was statistically significant after access cavity preparation (P ≤ 0.05).

Conclusion

Magnifying loupes significantly enhances the determination of the number and pattern of root canal orifices in primary teeth. Therefore, the application of magnifying loupes is essential for accurately assessing variations in root canal orifices in primary dentition.

Introduction

White spot lesions are opaque, chalky white or yellowish discolorations on the surface of teeth that result from the demineralization of the tooth structure. Many methods, including SEM, XRD, and FTIR spectroscopy, are crucial for identifying and evaluating enamel white spot lesions. It is imperative to have a thorough grasp of the morphology, crystallographic structure, mineral composition, and chemical changes associated with enamel white spot lesions.

Method

In vitro lesions were meticulously obtained by immersing extracted teeth in hydrochloric acid for a week and drying them using artificial caries. Characterization investigations were conducted with utmost precision and thoroughness using FTIR, XRD, and SEM, ensuring the reliability and validity of the results.

Results

FTIR analysis revealed the existence of calcium oxide and hydroxyapatite, and SEM examination assisted in identifying differences in surface shape. The enamel's crystalline nature was revealed via XRD investigation.

Conclusion

White spot lesions are associated with the development of deep caries. Advanced imaging strategies are needed for additional validation.

Objectives

The objective of the study was to propose and test a new grading system to quantify the clinical eruption of teeth into the oral cavity. In addition, the study also aimed to apply the grading system to a sample population to determine the chronology and sequence of permanent tooth eruptions, comparing the results with an existing standard table.

Methods

A cross-sectional study was designed, and 1220 children aged 5–18 years were selected from five schools in Chennai. The clinical status of permanent tooth eruption was graded using the newly proposed system. The sequence and chronology of permanent tooth eruptions were determined using Probit analysis and compared with those established by Logan and Kronfeld. The difference in stages of eruption between the sexes was analyzed using the ANOVA test.

Results

The study sample included 515 boys and 705 girls. A total of 23,218 permanent maxillary and mandibular teeth were examined and graded. Of the 11,085 maxillary teeth, 367 were classified as grade 1, 660 as grade 2, and 10,058 as grade 3. Similarly, of the 12,133 mandibular teeth, 497 were grade 1, 793 were grade 2, and 10,843 were grade 3. The eruption of the maxillary canines, second molars, and mandibular second premolars was observed to have occurred earlier than in the traditional table of tooth eruption. The most significant differences in the stages of eruption between the sexes were observed in the 9–12 age group.

Conclusions

The newly proposed grading system was found to be simple, objective, less confusing, and more robust, compared with the existing systems in determining the clinical status of tooth eruptions. The ages of eruption of maxillary canines, second molars, and mandibular second premolars were earlier.

Objective

This current study was designed to compare and correlate between smear layer eradication and reduction in microhardness by natural 0.2%chitosan nanoparticles and novel chemical irrigants with surfactant at the apical root third.

Materials and method

One hundred and twenty straight single-rooted extracted lower premolars were decoronated and working length obtained with #10 K-file. Pro-taper rotary files were used till apical size F3. The canals were simultaneously flushed with assigned irrigant containing surfactant [(ChX-Ultra, NaOCl-Extra, Pro-EDTA, 0.2%chitosan nanoparticles, Biopure MTAD]. The samples were randomly divided into two equal groups (n = 60). InGroup S (n = 60), the residual smear layer was examined by scanning electron microscope and in Group M(n = 60) microhardness was determined by Vickers Microhardness Tester. Further both groups were divided into six equal groups (n = 10 each) according to assigned irrigating solutions.

For smear layer removal; Kruskal-Wallis tests followed by pair wise comparison using Mann Whitney U test was done. For change in microhardness ANOVA and post hoc Tukey tests was done.

Results

Maximum smear layer removal was recorded in Biopure MTAD (1.8 ± 0.63), followed by pro-EDTA (2.2 0 ± 0.63) then 0.2%chitosan (2.6 ± 0.51), then NaOCL Extra (3.5 ± 0.53)and least in CHX-Ultra (4.4 ± 0.52) and saline (5.0 ± 0.00). Pro-EDTA group (12.8 ± 2.47) revealed significant highest reduction in microhardness followed by Biopure MTAD (8.01 ± 3.06), 0.2%chitosan nanoparticles (5.48 ± 2.87), NaOCl-Extra (5.44 ± 1.62) and least recorded in CHX-Ultra (4.94 ± 1.43) and saline (3.04 ± 0.63).

Conclusion

The elimination of the smear layer is always accompanied by a reduction in microhardness. Moreover, irrigant with surfactant and chelators enhanced smear layer removal, with best perceived in Biopure MTAD.

Introduction

Molar-incisor hypomineralization (MIH) is a localized, qualitative, demarcated enamel defect that affects first permanent molars (FPMs) and/or permanent incisors. The aim of present study was to introduce a novel computerised assessment process to detect and quantify the percentage opacity associated with MIH affected maxillary central incisors.

Methodology

Children (8–16 years) enrolled in the primary study having mild (white/cream or yellow/brown) MIH lesion on fully erupted maxillary permanent central incisor. 50 standardised images of MIH lesions were captured in an artificially lit room with fixed parameters and were anonymized and securely stored. Images were analysed by AI-driven computerised software and generates output classifications via a sophisticated algorithm crafted using a meticulously annotated image dataset as reference through supervised machine learning (SML). For the validation of computerised assessment of MIH lesions, the percentage of demarked opacity was calculated using ADOBE PHOTOSHOP CS7.

Results

The percentage of MIH lesion was calculated through histogram plotting with the maxima ranging from 7.29 % to 71.21 % with the mean value of 34.51 %. The validation score ranged from 10.29 % to 67.27 % with the mean value of 35.32 %. The difference between the two was statistically not significant. Out of 50 patients; 11 patients had 1–30 % of surface affected with MIH and 2 had aesthetic concern; 24 had 30–60 % of surface affected and 13 had aesthetic concern; 15 had >60 % of surface affected and 12 had aesthetic concerns.

Conclusions

The proposed approach exhibit sufficient quality to be integrated into a dental software addressing practical challenges encountered in daily clinical settings.

Our understanding of mesenchymal stem cells (MSCs) and their biological properties is steadily increasing, with more studies focusing on their therapeutic effects in the domains of immunology, tissue engineering and regenerative medicine. MSCs may be derived from tissues such as bone marrow, adipose, the umbilical cord, as well as from dental tissues (e.g., tooth germ, dental follicle, pulp tissue of exfoliated deciduous and permanent teeth, apical papilla, periodontal ligament, gingiva, and alveolar bone). Gingival mesenchymal stem cells (GMSCs) are non-hematopoietic adult stem cells isolated from the gingival lamina propria. When compared to MSCs purified from various dental and non-dental tissues, GMSCs are more abundant in source, relatively non-invasive to obtain, and genetically stable. In recent years, many studies have found that GMSCs possess the ability of self-renewal, multi-directional differentiation, and chemotaxis to inflammatory sites for immunity regulation. Their molecular and stem-cell properties make them highly suitable for both preclinical and clinical research. Extracellular vesicles (EVs) secreted by GMSCs are of key interest due to their ability to emulate the biological and therapeutic activity of GMSCs themselves. This paper will therefore review the current consensus on GMSCs, surveying their sources and isolation methods, their biological properties, and their therapeutic applications on inflammatory and immune-related diseases.

Spheroids are spherical aggregates of cells. Normally, most of adherent cells cannot survive in suspension; however, if they adhere to each other and grow to a certain size, they can survive without attaching to the dish surface. Studies have shown that spheroid formation induces dedifferentiation and improves plasticity, proliferative capability, and differentiation capability. In particular, spontaneous spheroids represent a selective and efficient cultivation technique for somatic stem cells. Organoids are considered mini-organs composed of multiple types of cells with extracellular matrices that are maintained in three-dimensional culture. Although their culture environment is similar to that of spheroids, organoids consist of differentiated cells with fundamental tissue/organ structures similar to those of native organs. Organoids have been used for drug development, disease models, and basic biological studies. Spheroid culture has been reported for various cell types in the oral and craniofacial regions, including salivary gland epithelial cells, periodontal ligament cells, dental pulp stem cells, and oral mucosa-derived cells. For broader clinical application, it is crucial to identify treatment targets that can leverage the superior stemness of spheroids. Organoids have been developed from various organs, including taste buds, oral mucosa, teeth, and salivary glands, for basic biological studies and also with the goal to replace damaged or defective organs. The development of novel immune-tolerant cell sources is the key to the widespread clinical application of organoids in regenerative medicine. Further efforts to understand the underlying basic mechanisms of spheroids and organoids will lead to the development of safe and efficient next-generation regenerative therapies.

Background

The progression and pathogenesis of oral cancer is greatly impacted by epigenetic modifications, such as DNA methylation. Autophagy, is an adaptive mechanism used to maintain the survival and integrity of cells. Oral squamous cell carcinoma is linked to a number of autophagy indicators, although it is yet unknown if DNA methylation of autophagy-related genes promotes the development of oral leukoplakia (OL), oral squamous cell carcinoma (OSCC).

Aim

Our study was aimed to assess, compare and evaluate the DNA methylation of ATG5 and MAP1LC3Av1 genes in oral leukoplakia, oral squamous cell carcinoma.

Materials and methods

This cross-sectional study was designed with sample size of 48 tissues which was clinically and histopathologically diagnosed as OL, OSCC and normal tissue. The samples were divided into three groups (Group A, Group B, and Group C; (n = 16 each). Following histopathological confirmation, the tissue was stored in the RNA reagent, then subjected to DNA extraction, methylation-sensitive polymerase chain reaction (MS-PCR). DNA methylation of the ATG5 and MAP1LC3Av1 genes were assessed.

Results

Shapiro-Wilk and Kolmogorov-Smirnov tests showed that the values were normally distributed. Both the ATG5 and MAP1LC3Av1 genes were methylated in OSCC, OL tissues compared to normal tissues. A statistically significant results was seen among the three study groups.

Conclusion

A significant difference was noted in the hypermethylation status of the promoter regions of the ATG5 and MAP1LC3Av1 genes. This provides some insight into their crucial role in the development of tumors. Future research with larger sample is needed to assess its potential clinical implications in oral carcinoma.

Early Childhood Caries (ECC) is a condition associated with nearly 123 risk factors. Among all the risk factors, enamel defects or enamel hypoplasia (ED/EH) has been shown to be the primary risk factor. ED/EH comprises the earliest changes that manifest on newly erupted primary tooth surfaces soon after its eruption. ED/EH appears as white lines or patches, either as demarcated or diffuse opacities on the enamel surface. It is often observed on the labial and buccal surfaces of the newly erupted primary maxillary incisors. A simple self-report tool for mothers/caretakers, healthcare professionals, and primary care workers to diagnose early changes in ECC may facilitate early diagnosis. The MAAC chart was developed to prevent ECC in children with cleft lip and palate. These charts, primarily developed to educate parents, caregivers, healthcare professionals, and primary health care providers, illustrate the variations in ED/EH on the enamel surface (demarcated and diffuse opacities on various maxillary teeth) as a collage of pictures organized as incisors and canines. This paper describes a novel and simple, tool called ‘MAAC charts’ providing guidance on early signs of Early childhood caries (ECC) for health professionals, parents and caretakers. MAAC charts can be useful for Pediatric dentists while providing anticipatory guidance during early first dental visits in their office. Pediatric dentists can suggest paediatricians to use these MAAC charts, in vaccinations clinics and various in/out patient settings to provide oral health related anticipatory guidance. Pediatric dentists can use these MAAC charts in large scale public health campaigns focused on preventing ECC.