Journal of oral biology and craniofacial research最新文献

英文中文

The role of rhBMP-2 in mandibular bone regeneration following tooth extraction through HIF-1α and VEGF-A expression: An Immunohistochemical study

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-13 DOI: 10.1016/j.jobcr.2025.02.001

Christian Khoswanto , Ira Kusuma Dewi

Background

Dentists frequently conduct tooth extractions when there is damage to the tooth or the tissue that supports it. When a tooth is extracted, the alveolar bone will sustain injury. Most of the initial bone volume is lost following the healing phase after extraction. Rehabilitation must start as soon as a tooth is missing, mainly because the alveolar bone is severely damaged during the tooth extraction, particularly in the buccal region where the tooth is removed. Dental implant is one method of replacing lost teeth. One of the most important elements influencing the clinical result of dental implants is a change in the dimension of the alveolar bone. Several bone-grafting techniques, such as socket preservation techniques, have been developed to increase the volume of bone throughout the healing phase after tooth extraction. This study aims to assess the impact rhBMP-2 on creating bone regeneration through VEGF and HIF-1α in the mandibular socket post-extraction in Wistar rats.

Method

On the anterior side of the mandible, in the socket area where the tooth was extracted, rhBMP-2 was injected into the socket, and the xenograft material was applied with a syringe. Male, 9-week-old Wistar rats were chosen (n = 30).

Result

Our statistical evaluations have revealed a significantly higher VEGF-A and HIF-1α expression post-extraction of the rhBMP-2 and xenograft group compared to other group treatments. These findings are significant as they provide a deeper understanding of the mechanisms involved in bone regeneration post-extraction.

Conclusion

Our study suggests that injecting rhBMP-2 into the grafted material and socket extraction during GBR dramatically increases the expression of VEGF-A and HIF-1α. These findings have the potential to significantly impact oral surgery and regenerative dentistry, opening up new possibilities for enhancing bone regeneration techniques.

{"title":"The role of rhBMP-2 in mandibular bone regeneration following tooth extraction through HIF-1α and VEGF-A expression: An Immunohistochemical study","authors":"Christian Khoswanto , Ira Kusuma Dewi","doi":"10.1016/j.jobcr.2025.02.001","DOIUrl":"10.1016/j.jobcr.2025.02.001","url":null,"abstract":"<div><h3>Background</h3><div>Dentists frequently conduct tooth extractions when there is damage to the tooth or the tissue that supports it. When a tooth is extracted, the alveolar bone will sustain injury. Most of the initial bone volume is lost following the healing phase after extraction. Rehabilitation must start as soon as a tooth is missing, mainly because the alveolar bone is severely damaged during the tooth extraction, particularly in the buccal region where the tooth is removed. Dental implant is one method of replacing lost teeth. One of the most important elements influencing the clinical result of dental implants is a change in the dimension of the alveolar bone. Several bone-grafting techniques, such as socket preservation techniques, have been developed to increase the volume of bone throughout the healing phase after tooth extraction. This study aims to assess the impact rhBMP-2 on creating bone regeneration through VEGF and HIF-1α in the mandibular socket post-extraction in Wistar rats.</div></div><div><h3>Method</h3><div>On the anterior side of the mandible, in the socket area where the tooth was extracted, rhBMP-2 was injected into the socket, and the xenograft material was applied with a syringe. Male, 9-week-old Wistar rats were chosen (n = 30).</div></div><div><h3>Result</h3><div>Our statistical evaluations have revealed a significantly higher VEGF-A and HIF-1α expression post-extraction of the rhBMP-2 and xenograft group compared to other group treatments. These findings are significant as they provide a deeper understanding of the mechanisms involved in bone regeneration post-extraction.</div></div><div><h3>Conclusion</h3><div>Our study suggests that injecting rhBMP-2 into the grafted material and socket extraction during GBR dramatically increases the expression of VEGF-A and HIF-1α. These findings have the potential to significantly impact oral surgery and regenerative dentistry, opening up new possibilities for enhancing bone regeneration techniques.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 359-364"},"PeriodicalIF":0.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of pulpal response at varying remaining dentin thickness in teeth restored with resin bulk fill composite, conventional glass ionomer cement and silver amalgam: Histomorphometric analysis

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-12 DOI: 10.1016/j.jobcr.2024.12.020

Ankita Agarwal , Promila Verma , Rakesh Yadav , Ramesh Bharti , Rhythm Bains , Madhu Kumar , Dipti Shastri

Objective

To compare and evaluate the pulp response to GC glass ionomer cement, SDR plus bulk fill composite and amalgam against gold standard calcium hydroxide cement at varying remaining dentin thickness, in teeth planned for orthodontic extraction.

Method

Thirty-eight human premolars were prepared with 2 mm or 2.5 mm depth cavities. They were restored with GC conventional glass ionomer cement, SDR plus bulk fill composite, amalgam, or lined with Dycal and restored with GIC. Two teeth were used as intact controls. After a 7-day interval, the teeth were extracted and processed for histological examination of the pulp and the thickness of the remaining dentin between the cavity floor and pulp tissue.

Results

All experimental groups showed some degree of inflammatory response. A significantly higher inflammatory response and more tissue disorganization were observed with SDR bulk fill composite (p < 0.05) compared to Glass ionomer cement, amalgam and Dycal at both cavity depths of 2 mm or 2.5 mm. The mean RDTs ranged from 346 μm to 1025 μm.

Conclusions

The study concluded that critical RDT varies for different restorative materials. It was observed that both glass ionomer cement and amalgam demonstrated acceptable biocompatibility when used in deep cavities. At the same time, SDR plus bulk fill composite proved to be the least biocompatible.

{"title":"Evaluation of pulpal response at varying remaining dentin thickness in teeth restored with resin bulk fill composite, conventional glass ionomer cement and silver amalgam: Histomorphometric analysis","authors":"Ankita Agarwal , Promila Verma , Rakesh Yadav , Ramesh Bharti , Rhythm Bains , Madhu Kumar , Dipti Shastri","doi":"10.1016/j.jobcr.2024.12.020","DOIUrl":"10.1016/j.jobcr.2024.12.020","url":null,"abstract":"<div><h3>Objective</h3><div>To compare and evaluate the pulp response to GC glass ionomer cement, SDR plus bulk fill composite and amalgam against gold standard calcium hydroxide cement at varying remaining dentin thickness, in teeth planned for orthodontic extraction.</div></div><div><h3>Method</h3><div>Thirty-eight human premolars were prepared with 2 mm or 2.5 mm depth cavities. They were restored with GC conventional glass ionomer cement, SDR plus bulk fill composite, amalgam, or lined with Dycal and restored with GIC. Two teeth were used as intact controls. After a 7-day interval, the teeth were extracted and processed for histological examination of the pulp and the thickness of the remaining dentin between the cavity floor and pulp tissue.</div></div><div><h3>Results</h3><div>All experimental groups showed some degree of inflammatory response. A significantly higher inflammatory response and more tissue disorganization were observed with SDR bulk fill composite (p < 0.05) compared to Glass ionomer cement, amalgam and Dycal at both cavity depths of 2 mm or 2.5 mm. The mean RDTs ranged from 346 μm to 1025 μm.</div></div><div><h3>Conclusions</h3><div>The study concluded that critical RDT varies for different restorative materials. It was observed that both glass ionomer cement and amalgam demonstrated acceptable biocompatibility when used in deep cavities. At the same time, SDR plus bulk fill composite proved to be the least biocompatible.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 347-354"},"PeriodicalIF":0.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tip up - Simplified technique for non-surgical rhinoplasty: A case series

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-12 DOI: 10.1016/j.jobcr.2025.01.013

Cláudia Almeida , Victor Rogerio , Gabriela Giro , Victor Munoz-Lora , Marcelo Germani

Non-surgical nasal rhinoplasty using hyaluronic acid (HA) is a technique that has been widely adopted due to its minimally invasive and reversible nature. Reshaping the nose with HA is becoming increasingly popular for its quick and safe outcomes, serving as an effective alternative for correcting minor nasal deformities. The proposed approach, named the Tip Up (TU) technique, focuses on strategic injection sites, specifically in the Nasal Tip region, Nasal Spine, and Columella, utilizing minimal amounts of the product to minimize risks and enhance the predictability of the results. Procedures are performed under anesthesia without vasoconstrictors to prevent ischemia, and careful selection of the product ensures subtle and precise corrections. This case series details all aspects of the TU technique, highlighting the satisfactory and predictable aesthetic results achieved through a safe and reliable procedure.

引用次数: 0

Bioprinting techniques for regeneration of oral and craniofacial tissues: Current advances and future prospects

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-12 DOI: 10.1016/j.jobcr.2025.01.019

Shailesh Varshney , Anshuman Dwivedi , Vibha Pandey

Background

Regenerative dentistry aims to reinstate, fix, renew, and regrow tissues within the oral and craniofacial domain. Existing regenerative methods are based on insights into tissue biology or disease processes that lead to tissue degradation. However, achieving complete and functional Tissue regeneration remains a primary challenge in real-world medical scenarios.

Aim

The review focuses on the application of bioprinting techniques for rejuvenating intricate Oral and craniofacial tissues, such as craniofacial bone, periodontal ligament, cementum, dental pulp, temporomandibular joint cartilage, and whole teeth.

Methods

Bioprinting, a cutting-edge technology in regenerative dentistry, strives to create entirely new Functional tissues and organs. This approach merges principles from engineering and biology to produce three-dimensional biologically operational constructs containing bioactive substances, Living cells and cell clusters using automated bioprinters. The review summarizes the outcomes achieved through bioprinting techniques in both in vitro (laboratory experiments) and in vivo (Studies on living organisms) experiments.

Result

The emergence of this innovative tissue engineering technology has yielded highly promising outcomes during the experimental stages.

Conclusion

These promising experimental results necessitate replication through human clinical trials to ascertain the viability of bioprinting techniques for mainstream clinical implementation in regenerative dentistry.

{"title":"Bioprinting techniques for regeneration of oral and craniofacial tissues: Current advances and future prospects","authors":"Shailesh Varshney , Anshuman Dwivedi , Vibha Pandey","doi":"10.1016/j.jobcr.2025.01.019","DOIUrl":"10.1016/j.jobcr.2025.01.019","url":null,"abstract":"<div><h3>Background</h3><div>Regenerative dentistry aims to reinstate, fix, renew, and regrow tissues within the oral and craniofacial domain. Existing regenerative methods are based on insights into tissue biology or disease processes that lead to tissue degradation. However, achieving complete and functional Tissue regeneration remains a primary challenge in real-world medical scenarios.</div></div><div><h3>Aim</h3><div>The review focuses on the application of bioprinting techniques for rejuvenating intricate Oral and craniofacial tissues, such as craniofacial bone, periodontal ligament, cementum, dental pulp, temporomandibular joint cartilage, and whole teeth.</div></div><div><h3>Methods</h3><div>Bioprinting, a cutting-edge technology in regenerative dentistry, strives to create entirely new Functional tissues and organs. This approach merges principles from engineering and biology to produce three-dimensional biologically operational constructs containing bioactive substances, Living cells and cell clusters using automated bioprinters. The review summarizes the outcomes achieved through bioprinting techniques in both in vitro (laboratory experiments) and in vivo (Studies on living organisms) experiments.</div></div><div><h3>Result</h3><div>The emergence of this innovative tissue engineering technology has yielded highly promising outcomes during the experimental stages.</div></div><div><h3>Conclusion</h3><div>These promising experimental results necessitate replication through human clinical trials to ascertain the viability of bioprinting techniques for mainstream clinical implementation in regenerative dentistry.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 331-346"},"PeriodicalIF":0.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

“Effect of oral systemic administration of vitamin D on the rate of maxillary canine retraction: A randomized controlled trial”

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-11 DOI: 10.1016/j.jobcr.2025.01.017

Davender Kumar , Revathi Mn , Rekha Sharma , Ashuma Sachdeva , Nameksh Raj Bhupali , Ravinder Solanki

Objective

The trial was conducted to determine the effects of systemically delivered Vitamin D (1,25 dihydroxycholecalciferol) on the rate of maxillary canine retraction till the space closure and on the root resorption.

Materials and methods

A two-arm parallel randomized controlled trial was conducted in patients with Angle's Class I bimaxillary protrusion requiring at least upper first premolars extraction and distal movement of canine for malocclusion correction. The enrolled patients were randomized and allocated to the experimental group (Oral Vitamin D = 0.25 μg given) and control group (Placebo given). The canine retraction was initiated using nickel-titanium (NiTi) closed coil springs delivering a force of 100 gm per side and Vitamin D levels were monitored at monthly intervals. The patients' casts were digitally scanned and examined for differences in the rate of canine retraction at initial (T0), 4 weeks (T1), 8 weeks (T2), 12 weeks (T3), 16 weeks (T4) and 20 weeks (T5) intervals were calculated. The volumetric root resorption was done on CBCT of the area of interest at T0 and after completion of retraction. Descriptive statistics and paired t-test were used to determine any differences.

Results

32 patients (18–24 years) were randomized in the experimental group (n = 16) and control group (n = 16) and no dropout was noted till the end of the study. The results showed a statistically significant increase in the rate of canine retraction in the experimental group as compared to the control group at different time intervals. The differences in the mean canine retraction between group 1 and group 2 at T1-T0, T2-T1, T3-T2, T4-T3, and T4-T0 were 0.28 ± 0.12, 0.29 ± 0.10, 0.31 ± 0.08, 0.37 ± 0.06 and 1.18 ± 0.10 mm respectively. The total mean canine retraction for group 1 was achieved at T4 time interval while it was achieved at T5 interval for group 2. The intergroup comparison of maxillary canine roots showed no statistically significant difference in volumetric root resorption.

Conclusion

The active form of vitamin D can be an effective agent to accelerate orthodontic tooth movement (OTM).

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引用次数: 0

Antibiofilm efficiency of silver and copper nanoparticle incorporated calcium hydroxide as an intracanal medicament: An in vitro study

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-11 DOI: 10.1016/j.jobcr.2025.01.020

Murali H. Rao , Krishnan Rajkumar , Gopal Pavithra , Ramya Ramadoss , Suryanarayanan Shankar , Krishnamachari Janani , Kumar Chandan Srivastava , Deepti Shrivastava , Dinesh Rokaya

Background and objectives

Silver (Ag) and Copper (Cu) Nanoparticles (NP) are a potential substitute for disinfection during endodontic therapy because of their antibacterial action against an array of pathogens, including resistant strains. This study aimed to assess the antibiofilm efficiency of calcium hydroxide modified with Ag and Cu NP suspension against E. faecalis, using confocal laser scanning microscopy (CLSM) and colony forming units (CFU) analysis.

Methods

Dentine blocks were inoculated with Enterococcus faecalis for one week and 1 week-old biofilm of E. feacalis were randomly divided into 4 groups, control, calcium hydroxide, calcium hydroxide +2 % Cu NP and calcium hydroxide +2 % Ag NP. Following the incubation of the specimens with the medicament for 24 h at 37C, CLSM was used to evaluate the reduction in biovolume of the biofilm and the CFU was determined to assess the antimicrobial action of the NP-modified calcium hydroxide.

Results

CLSM images revealed a significant reduction in the thickness of biofilm in both Ag and Cu groups compared to calcium hydroxide alone. The CFU results showed that group 4 and group 3 showed significantly less CFU followed by group 2 and group 1 (p < 0.001).

Conclusion

Adding Cu and Ag NP to calcium hydroxide intracanal medication significantly increases its antibacterial activity against E. faecalis.

{"title":"Antibiofilm efficiency of silver and copper nanoparticle incorporated calcium hydroxide as an intracanal medicament: An in vitro study","authors":"Murali H. Rao , Krishnan Rajkumar , Gopal Pavithra , Ramya Ramadoss , Suryanarayanan Shankar , Krishnamachari Janani , Kumar Chandan Srivastava , Deepti Shrivastava , Dinesh Rokaya","doi":"10.1016/j.jobcr.2025.01.020","DOIUrl":"10.1016/j.jobcr.2025.01.020","url":null,"abstract":"<div><h3>Background and objectives</h3><div>Silver (Ag) and Copper (Cu) Nanoparticles (NP) are a potential substitute for disinfection during endodontic therapy because of their antibacterial action against an array of pathogens, including resistant strains. This study aimed to assess the antibiofilm efficiency of calcium hydroxide modified with Ag and Cu NP suspension against <em>E. faecalis</em>, using confocal laser scanning microscopy (CLSM) and colony forming units (CFU) analysis.</div></div><div><h3>Methods</h3><div>Dentine blocks were inoculated with Enterococcus faecalis for one week and 1 week-old biofilm of <em>E. feacalis</em> were randomly divided into 4 groups, control, calcium hydroxide, calcium hydroxide +2 % Cu NP and calcium hydroxide +2 % Ag NP. Following the incubation of the specimens with the medicament for 24 h at 37C, CLSM was used to evaluate the reduction in biovolume of the biofilm and the CFU was determined to assess the antimicrobial action of the NP-modified calcium hydroxide.</div></div><div><h3>Results</h3><div>CLSM images revealed a significant reduction in the thickness of biofilm in both Ag and Cu groups compared to calcium hydroxide alone. The CFU results showed that group 4 and group 3 showed significantly less CFU followed by group 2 and group 1 (p < 0.001).</div></div><div><h3>Conclusion</h3><div>Adding Cu and Ag NP to calcium hydroxide intracanal medication significantly increases its antibacterial activity against <em>E. faecalis</em>.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 319-324"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal efficacy of chitosan-mediated fenugreek nanocomposite incorporated in tissue conditioner

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-11 DOI: 10.1016/j.jobcr.2025.01.007

Mohika Damle, Ponsekar Abraham Anandapandian, B. Eswaran, Swapnaneel Pradhan, Silvin Joe C J, Shreyas Dhumke

Introduction

The rough surface of dentures, coupled with xerostomia and immunosuppression, fosters microbial growth and colonization, with Candida albicans being a primary culprit. Tissue conditioners, used to reline ill-fitting dentures, can temporarily alleviate discomfort but are prone to microbial colonization. To address this, researchers have explored incorporating antifungal agents directly into tissue conditioners. Nanoparticles, particularly green-synthesized ones derived from fenugreek, have shown significant antifungal efficacy. Combining fenugreek extract with chitosan, can further improve antifungal outcomes due to its excellent drug delivery, mucoadhesive properties, and bioavailability.

Aim

To synthesize Chitosan mediated Fenugreek nanocomposite (1.5 %,2 %,2.5 % by weight concentrations) and evaluate its antifungal efficacy on heat cure denture base relined with tissue conditioner mixed with nanocomposite by assessing fungal colony formation after 1st day, 1st week and 2nd week.

Methodology

After synthesizing chitosan mediated fenugreek Nanocomposite, its characterization was performed using SEM, FTIR analysis and UV–Vis spectroscopy. Heat cure denture base discs coated with tissue conditioner-nanocomposite mixture were used to transfer fungal cultures onto Rose Bengal agar to assess colony formation.

Results and discussion

Chitosan-fenugreek nanoparticles were synthesized using the ionic gelation method, with fenugreek extract used for green synthesis. Kruskal-Wallis test showed significant effects over time, with 2.5 % group showing lowest fungal growth at 521 CFU/mL, followed by 2 % group at 587 CFU/mL, while the 1.5 % nanocomposite group still displayed considerable fungal growth.

Conclusion

Incorporating chitosan-mediated fenugreek nanocomposite into denture tissue conditioners offers a promising approach to reducing Candidal growth, potentially improving oral health outcomes for denture wearers.

{"title":"Antifungal efficacy of chitosan-mediated fenugreek nanocomposite incorporated in tissue conditioner","authors":"Mohika Damle, Ponsekar Abraham Anandapandian, B. Eswaran, Swapnaneel Pradhan, Silvin Joe C J, Shreyas Dhumke","doi":"10.1016/j.jobcr.2025.01.007","DOIUrl":"10.1016/j.jobcr.2025.01.007","url":null,"abstract":"<div><h3>Introduction</h3><div>The rough surface of dentures, coupled with xerostomia and immunosuppression, fosters microbial growth and colonization, with <em>Candida albicans</em> being a primary culprit. Tissue conditioners, used to reline ill-fitting dentures, can temporarily alleviate discomfort but are prone to microbial colonization. To address this, researchers have explored incorporating antifungal agents directly into tissue conditioners. Nanoparticles, particularly green-synthesized ones derived from fenugreek, have shown significant antifungal efficacy. Combining fenugreek extract with chitosan, can further improve antifungal outcomes due to its excellent drug delivery, mucoadhesive properties, and bioavailability<strong>.</strong></div></div><div><h3>Aim</h3><div>To synthesize Chitosan mediated Fenugreek nanocomposite (1.5 %,2 %,2.5 % by weight concentrations) and evaluate its antifungal efficacy on heat cure denture base relined with tissue conditioner mixed with nanocomposite by assessing fungal colony formation after 1st day, 1st week and 2nd week.</div></div><div><h3>Methodology</h3><div>After synthesizing chitosan mediated fenugreek Nanocomposite, its characterization was performed using SEM, FTIR analysis and UV–Vis spectroscopy. Heat cure denture base discs coated with tissue conditioner-nanocomposite mixture were used to transfer fungal cultures onto Rose Bengal agar to assess colony formation.</div></div><div><h3>Results and discussion</h3><div>Chitosan-fenugreek nanoparticles were synthesized using the ionic gelation method, with fenugreek extract used for green synthesis. Kruskal-Wallis test showed significant effects over time, with 2.5 % group showing lowest fungal growth at 521 CFU/mL, followed by 2 % group at 587 CFU/mL, while the 1.5 % nanocomposite group still displayed considerable fungal growth.</div></div><div><h3>Conclusion</h3><div>Incorporating chitosan-mediated fenugreek nanocomposite into denture tissue conditioners offers a promising approach to reducing Candidal growth, potentially improving oral health outcomes for denture wearers.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 271-276"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of cyclic fatigue in three pediatric endodontic rotary file systems in root canals of primary molars: A finite element analysis (FEA)

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-11 DOI: 10.1016/j.jobcr.2025.01.023

S.S. Monika sri, K.C. Vignesh, K. Vivek, Kavitha Swaminathan, Selvakumar Haridoss

Objective

The aim of this study was to evaluate the cyclic fatigue resistance of three different pediatric endodontic rotary files in the root canals of primary molars using finite element analysis (FEA). The research focuses on understanding the mechanical behavior of the files under varying canal curvatures to determine their suitability for pediatric endodontics.

Methods

Finite Element Analysis (FEA) was utilized to simulate cyclic fatigue performance in primary molar canals with curvatures of 30°, 60°, and 90°. The rotary files made up of nickel-titanium (NiTi) alloys included in this study were Kedo SG, Neoendo Pedo Flex, and Pro AF Baby. Stress distribution, maximum stress values, and cyclic fatigue resistance were evaluated to identify differences in performance. Simulations were conducted under controlled conditions to model real-world clinical scenarios. Stress distribution and the number of cycles until failure were analyzed using Goodman's fatigue model and the S-N curve.

Results

The Pro AF Baby files exhibited the highest cyclic fatigue resistance and the more favourable stress distribution across all canal profiles, due to its triangular cross-sectional geometry. Whereas, Kedo SG and Neoendo Pedo Flex demonstrated higher stress levels and lower fatigue life, particularly in severe curvatures (90°), indicating increased susceptibility to failure. Elevated stress concentrations were observed near the apical tip, correlating with reduced fatigue life as canal curvature increased.

Conclusion

Pro AF Baby B2 demonstrated better fatigue resistance and safety for endodontic procedures in primary molars with complex root canal curvatures. These findings emphasize the importance of material properties and file design in improving clinical outcomes. Future research should focus on long-term clinical validation and further optimization of rotary file systems for pediatric dentistry.

{"title":"Evaluation of cyclic fatigue in three pediatric endodontic rotary file systems in root canals of primary molars: A finite element analysis (FEA)","authors":"S.S. Monika sri, K.C. Vignesh, K. Vivek, Kavitha Swaminathan, Selvakumar Haridoss","doi":"10.1016/j.jobcr.2025.01.023","DOIUrl":"10.1016/j.jobcr.2025.01.023","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to evaluate the cyclic fatigue resistance of three different pediatric endodontic rotary files in the root canals of primary molars using finite element analysis (FEA). The research focuses on understanding the mechanical behavior of the files under varying canal curvatures to determine their suitability for pediatric endodontics.</div></div><div><h3>Methods</h3><div>Finite Element Analysis (FEA) was utilized to simulate cyclic fatigue performance in primary molar canals with curvatures of 30°, 60°, and 90°. The rotary files made up of nickel-titanium (NiTi) alloys included in this study were Kedo SG, Neoendo Pedo Flex, and Pro AF Baby. Stress distribution, maximum stress values, and cyclic fatigue resistance were evaluated to identify differences in performance. Simulations were conducted under controlled conditions to model real-world clinical scenarios. Stress distribution and the number of cycles until failure were analyzed using Goodman's fatigue model and the S-N curve.</div></div><div><h3>Results</h3><div>The Pro AF Baby files exhibited the highest cyclic fatigue resistance and the more favourable stress distribution across all canal profiles, due to its triangular cross-sectional geometry. Whereas, Kedo SG and Neoendo Pedo Flex demonstrated higher stress levels and lower fatigue life, particularly in severe curvatures (90°), indicating increased susceptibility to failure. Elevated stress concentrations were observed near the apical tip, correlating with reduced fatigue life as canal curvature increased.</div></div><div><h3>Conclusion</h3><div>Pro AF Baby B2 demonstrated better fatigue resistance and safety for endodontic procedures in primary molars with complex root canal curvatures. These findings emphasize the importance of material properties and file design in improving clinical outcomes. Future research should focus on long-term clinical validation and further optimization of rotary file systems for pediatric dentistry.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 310-318"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined tracheostomy and maxillomandibular advancement for extremely severe obstructive sleep apnea: A case report

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-11 DOI: 10.1016/j.jobcr.2025.01.028

Ning Zhou , Jean-Pierre T.F. Ho , Jan de Lange

Tracheostomy and maxillomandibular advancement (MMA) are both highly effective surgical treatments for adult obstructive sleep apnea (OSA). The present case study described the successful application of combined tracheostomy and MMA for the treatment of extremely severe OSA.

引用次数: 0

Exploring the predictive power of antinuclear antibodies and Rheumatoid factor correlations in anticipating therapeutic outcomes for female patients with coexisting Sjögren's syndrome and Rheumatoid arthritis

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-11 DOI: 10.1016/j.jobcr.2025.01.012

Anitha Krishnan Pandarathodiyil , Hema Shree K , Pratibha Ramani , Sivapathasundharam B. , Ramya Ramadoss

Background

Sjögren's Syndrome (SS) and Rheumatoid Arthritis (RA) are autoimmune conditions that often coexist in female patients. Biomarkers such as antinuclear antibodies (ANA) and rheumatoid factor (RF) are used for diagnosis, but their predictive power for treatment outcomes remains unclear. This study aims to investigate the correlation between age, ANA, RF, and treatment response in female patients with both SS and RA.

Objective

To evaluate the relationships between age, ANA, RF levels, RA (disease present), and treatment response using Pearson correlation analysis and a neural network model, to predict treatment outcomes in patients with coexisting SS and RA.

Methods

A cohort of 56 female patients aged 30–73 was analyzed. Descriptive statistics provided an overview of key variables, followed by Pearson correlation analysis to assess relationships between age, ANA, RF, RA, and treatment response. A neural network model was developed to predict treatment response based on age, ANA, and RF levels, using a training-to-testing split of 81.3 % and 18.8 %, respectively.

Results

The Pearson correlation analysis revealed a significant positive correlation between age and ANA levels (r = .541, p = 0.031), though no significant correlations were found between age, RF, RA, and treatment response. The neural network model achieved an accuracy of 92.3 % during training and 100 % accuracy during testing for most treatment categories. However, the model struggled to accurately distinguish between certain classes, particularly treatment categories 1 and 3.

Conclusion

Age showed a significant correlation with ANA levels, indicating that older patients may have elevated ANA. The neural network model demonstrated strong predictive power for treatment response, although further refinement is needed to improve its ability to distinguish between all response categories. These findings suggest that machine learning models could enhance personalized treatment strategies for patients with SS and RA, but additional validation with larger datasets is required.

{"title":"Exploring the predictive power of antinuclear antibodies and Rheumatoid factor correlations in anticipating therapeutic outcomes for female patients with coexisting Sjögren's syndrome and Rheumatoid arthritis","authors":"Anitha Krishnan Pandarathodiyil , Hema Shree K , Pratibha Ramani , Sivapathasundharam B. , Ramya Ramadoss","doi":"10.1016/j.jobcr.2025.01.012","DOIUrl":"10.1016/j.jobcr.2025.01.012","url":null,"abstract":"<div><h3>Background</h3><div>Sjögren's Syndrome (SS) and Rheumatoid Arthritis (RA) are autoimmune conditions that often coexist in female patients. Biomarkers such as antinuclear antibodies (ANA) and rheumatoid factor (RF) are used for diagnosis, but their predictive power for treatment outcomes remains unclear. This study aims to investigate the correlation between age, ANA, RF, and treatment response in female patients with both SS and RA.</div></div><div><h3>Objective</h3><div>To evaluate the relationships between age, ANA, RF levels, RA (disease present), and treatment response using Pearson correlation analysis and a neural network model, to predict treatment outcomes in patients with coexisting SS and RA.</div></div><div><h3>Methods</h3><div>A cohort of 56 female patients aged 30–73 was analyzed. Descriptive statistics provided an overview of key variables, followed by Pearson correlation analysis to assess relationships between age, ANA, RF, RA, and treatment response. A neural network model was developed to predict treatment response based on age, ANA, and RF levels, using a training-to-testing split of 81.3 % and 18.8 %, respectively.</div></div><div><h3>Results</h3><div>The Pearson correlation analysis revealed a significant positive correlation between age and ANA levels (r = .541, p = 0.031), though no significant correlations were found between age, RF, RA, and treatment response. The neural network model achieved an accuracy of 92.3 % during training and 100 % accuracy during testing for most treatment categories. However, the model struggled to accurately distinguish between certain classes, particularly treatment categories 1 and 3.</div></div><div><h3>Conclusion</h3><div>Age showed a significant correlation with ANA levels, indicating that older patients may have elevated ANA. The neural network model demonstrated strong predictive power for treatment response, although further refinement is needed to improve its ability to distinguish between all response categories. These findings suggest that machine learning models could enhance personalized treatment strategies for patients with SS and RA, but additional validation with larger datasets is required.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 288-296"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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