Journal of oral biology and craniofacial research最新文献_第2页

Effect of surface preparation methods on the shear bond strength of universal and self-etch adhesive systems: an in vitro study 表面处理方法对通用和自蚀刻胶粘剂系统剪切结合强度的影响：体外研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-19 DOI: 10.1016/j.jobcr.2026.101408

Shiwangi Verma , Ravishankar Suman , Rajendra Goud , Snigdho Das , Srikant Gollapudi , Samapika Routray

Objectives

To assess the impact of three smear layer preparation methods on shear bond strength (SBS) to enamel and dentin, using two universal adhesives (Tetric N-Bond Universal, 3M Single Bond Universal) and one two-step self-etch adhesive (Kuraray Clearfil SE Bond).

Methods

Ninety extracted premolars were assigned to enamel and dentin groups (n = 45 each) and prepared with coarse grit (120), fine grit (600) silicon carbide paper, or air abrasion (Aluminium Oxide for enamel, Sylc for dentin). Surfaces were bonded with one of the adhesives and restored with composite. Enamel was treated in an etch-and-rinse mode, dentin in self-etch mode. SBS was tested using a universal testing machine. Failure surfaces were examined under SEM to assess fracture morphology and EDS to determine elemental composition. Data were analyzed using two-way ANOVA/Tukey's post-hoc test and Weibull statistics (P ≤ 0.05).

Results

In enamel, SBS generally increased from coarse to fine to air abrasion. Kuraray Clearfil SE Bond yielded the highest values, particularly with air abrasion (9.11 ± 0.51 MPa). In dentin, fine grit achieved the highest SBS across all adhesives, followed by air abrasion; coarse grit showed the lowest. Kuraray consistently outperformed the universal adhesives in most dentin conditions. SEM/EDS revealed predominantly adhesive failures; occasional cohesive failures in dentin (Ivoclar) and enamel (Kuraray) corresponded with higher SBS and Weibull reliability.

Conclusion

Both surface preparation and adhesive type significantly influence SBS. Kuraray showed superior performance and reliability, while fine grit and air abrasion enhanced bonding efficacy. SEM/EDS findings corroborated the mechanical results.

目的采用两种通用胶粘剂（Tetric N-Bond universal、3M Single bond universal）和一种两步自蚀刻胶粘剂（Kuraray Clearfil SE bond），评价三种涂抹层制备方法对牙釉质和牙本质剪切结合强度（SBS）的影响。方法将90颗拔除的前磨牙分为牙本质组和牙釉质组，每组45颗，分别用粗磨（120颗）、细磨（600颗）碳化硅纸或空气磨蚀（氧化铝磨蚀牙釉质，硫胺磨蚀牙本质）进行磨蚀。用其中一种粘合剂粘合表面，并用复合材料修复。牙釉质采用蚀刻-冲洗模式，牙本质采用自蚀刻模式。使用通用试验机对SBS进行了测试。在扫描电镜和能谱下对断裂表面进行了检查，以评估断裂形态和确定元素组成。数据分析采用双因素方差分析/Tukey事后检验和威布尔统计（P≤0.05）。结果在牙釉质中，SBS由粗到细再到气蚀逐渐增加。Kuraray Clearfil SE Bond获得了最高的值，特别是在空气磨损时（9.11±0.51 MPa）。在牙本质中，细砂在所有粘合剂中SBS最高，其次是空气磨损；粗粒度最低。在大多数牙本质条件下，Kuraray始终优于通用粘接剂。SEM/EDS主要显示胶粘剂失效；牙本质（Ivoclar）和牙釉质（Kuraray）偶尔的内聚失败与较高的SBS和Weibull可靠性相对应。结论表面处理和黏合剂类型对SBS均有显著影响。Kuraray表现出优异的性能和可靠性，而细粒度和空气磨损则增强了粘接效果。SEM/EDS结果证实了力学结果。

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引用次数: 0

Economic burden and economic impact of oral diseases in India: A systematic review and meta-analysis 印度口腔疾病的经济负担和经济影响：系统回顾和荟萃分析

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-19 DOI: 10.1016/j.jobcr.2026.01.006

Vineetha Karuveettil , Chandrashekar Janakiram , Aparna Ramachandran , Denny John

Objective

To quantify the economic burden and financial impact of oral diseases in India—on individuals, households, the health system, and society—focusing on direct and indirect costs and out-of-pocket expenditure (OOPE).

Methods

Following JBI methodology and PRISMA 2020, we conducted a three-step search of MEDLINE, Embase, Scopus, CINAHL, DOSS, Cochrane CENTRAL, and grey literature. We included India-based studies reporting economic burden/financial impact at individual, household, or population levels. Eligible designs were cost-of-illness, cost/expenditure analyses, and cohort or cross-sectional studies; mixed-methods with extractable quantitative data were included. Two reviewers independently screened, appraised (JBI tools), and extracted data on direct/indirect costs, OOPE, catastrophic expenditure (CHE), and hardship financing. Findings were narratively synthesized; meta-analysis was undertaken in JBI SUMARI where appropriate.

Results

Of 1684 records, 15 met inclusion criteria, mostly cross-sectional across states. OOPE varied widely; oral-cancer treatment often ranged from INR 80,000 to over INR 2,30,000. Direct costs were higher in private settings; indirect productivity losses were reported. CHE ranged from 0.6 % to 96 % across definitions; pooling studies using the ≥20 % income threshold yielded a CHE prevalence of 18.8 %. Insurance coverage was low (<15 %) with limited protection. Up to one-third of patients relied on hardship financing (borrowing or asset sales).

Conclusion

Oral diseases impose a substantial economic burden in India, characterised by high OOPE, elevated CHE risk (pooled 18.8 %), and frequent distress financing. Priorities include stronger national data, standardised costing/reporting (price year, components, variance), and expanded financial protection to reduce household hardship and advance universal oral health coverage.

Systematic review registration number

The protocol is prospectively registered in the International Prospective Register of Systematic Reviews database (PROSPERO ID: CRD420251030651).

目的量化印度口腔疾病对个人、家庭、卫生系统和社会的经济负担和财政影响，重点关注直接和间接成本和自付费用（OOPE）。方法采用JBI方法学和PRISMA 2020，对MEDLINE、Embase、Scopus、CINAHL、DOSS、Cochrane CENTRAL和灰色文献进行三步检索。我们纳入了以印度为基础的研究，报告了个人、家庭或人口水平上的经济负担/财务影响。符合条件的设计是疾病成本、成本/支出分析和队列或横断面研究；包括可提取定量数据的混合方法。两位审稿人独立筛选、评估（JBI工具），并提取了直接/间接成本、OOPE、灾难性支出（CHE）和困难融资方面的数据。研究结果以叙述方式综合；适当时在JBI SUMARI中进行meta分析。结果在1684份记录中，有15份符合纳入标准，大部分是跨州的横截面记录。OOPE变化很大；口腔癌的治疗费用通常在8万卢比到23万卢比以上。私人部门的直接成本更高；有间接生产力损失的报告。不同定义的CHE范围从0.6%到96%；采用≥20%收入阈值的合并研究得出CHE患病率为18.8%。保险覆盖率很低（15%），保障有限。多达三分之一的患者依靠困难融资（借款或出售资产）。结论口腔疾病在印度造成了巨大的经济负担，其特点是口腔疾病发生率高，CHE风险升高（合计18.8%），以及频繁的紧急融资。优先事项包括加强国家数据、标准化成本核算/报告（价格年度、组成部分、差异）和扩大财务保护，以减少家庭困难和推进全民口腔健康覆盖。该方案已在国际前瞻性系统评价注册数据库（PROSPERO ID: CRD420251030651）中前瞻性注册。

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引用次数: 0

Comparison of effectiveness of hyaluronic acid and melatonin with guided tissue regeneration (GTR) membrane versus guided tissue regeneration (GTR) membrane alone in the treatment of intra-bony defects in human subjects: A split-mouth randomised controlled clinical trial 透明质酸和褪黑素联合引导组织再生膜（GTR）与单独引导组织再生膜（GTR）治疗人类骨内缺损的疗效比较：一项裂口随机对照临床试验

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-16 DOI: 10.1016/j.jobcr.2026.01.001

Subasree Soundarajan, Sankari Malaiappan

Background

Melatonin is one substance that is primarily generated by the pineal gland and numerous other tissues and has vital involvement in many physiological processes, including bone remodeling. Over the last several years, another emerging molecule serving as a potential candidate for periodontal regeneration is the hyaluronic acid (HA), a key extracellular matrix component involved in cell migration. The aim of this study is the additive effect of hyaluronic acid and melatonin with GTR membrane in intrabony defects among patients with chronic periodontitis.

Materials and methods

15 bilateral intra-bony defects were treated either with Hyaluronic acid and melatonin along with GTR membrane (Group I), or GTR membrane alone (Group II). Clinical and radiographic parameters assessed were pocket depth, clinical attachment level, and volume of bone defect at baseline and 6 months after the intervention. The biochemical parameter assessed was Matrix metalloproteinase - 1 (MMP-1) and Tissue inhibitor of metalloproteinase (TIMP-1) at baseline and one month after the intervention.

Results

Both the study groups showed statistically significant differences in all the parameters from baseline to follow-up. However, group 1 (Hyaluronic acid + Melatonin + GTR membrane) showed statistically significant improvement in all the study parameters when compared to group 2 (GTR alone).

Conclusion

It can be concluded that the combination of hyaluronic acid and melatonin was effective in the regeneration of intrabony defects among patients with chronic periodontitis. However, further studies with a longer time period and with additional microbiological assessment would be desirable so as to substantiate the current results.

褪黑素是一种主要由松果体和许多其他组织产生的物质，在许多生理过程中起重要作用，包括骨重塑。在过去的几年里，另一个作为牙周再生的潜在候选分子是透明质酸（HA），它是参与细胞迁移的关键细胞外基质成分。本研究的目的是观察透明质酸和褪黑素与GTR膜对慢性牙周炎患者骨内缺损的加性作用。材料与方法15例双侧骨内缺损采用透明质酸、褪黑素联合GTR膜治疗（I组），GTR膜治疗（II组）。评估的临床和影像学参数包括基线和干预后6个月时的骨袋深度、临床附着水平和骨缺损体积。在基线和干预后1个月评估基质金属蛋白酶-1 （MMP-1）和金属蛋白酶组织抑制剂（TIMP-1）的生化参数。结果两个研究组从基线到随访的所有参数均有统计学差异。然而，与2组（单独使用GTR）相比，1组（透明质酸+褪黑素+ GTR膜）在所有研究参数上均有统计学意义的改善。结论透明质酸联合褪黑素治疗慢性牙周炎骨内缺损有较好的再生效果。然而，为了证实目前的结果，需要进行更长的时间和额外的微生物评估的进一步研究。

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引用次数: 0

Regional odontodysplasia: a report of unusual case with in-depth analysis 局部牙发育不良：罕见病例报告并深入分析

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-15 DOI: 10.1016/j.jobcr.2025.12.012

Charles Béhot , Ophélie Cuisinier , Éden Serraf , Lisa Friedlander , Brigitte Vi-Fane , Pascal Garrec , Ali Nassif

Background/purpose

Regional odontodysplasia (RO) is a rare developmental anomaly affecting dental tissue formation, typically confined to a single quadrant. It manifests through hypoplastic enamel, irregular dentin, short roots with open apices, and a characteristic “ghost-like” radiographic appearance. Its etiology remains unclear, with vascular, genetic and inflammatory factors proposed.

Materials and methods

In this study, we report the case of a 15-year-old female with mandibular RO affecting canine, premolars, and molars, focusing on the clinical, radiographic, and microscopic analysis of the affected teeth, including mineral density evaluation.

Results

The 2nd premolar and first and second molars (teeth 35, 36, 37) exhibited severe morphological defects and were extracted due to extreme root shortening (40 %, 90 %, and >95 % reduction), while alveolar bone height remained preserved. The canine and 1st premolar were affected to a lesser extent and were conserved upon orthodontic treatment plan. Scanning electron microscopy revealed slight decreases in the number and diameter of the dentinal tubules in the affected teeth compared with those in the control teeth. Contrary to many reported cases of RO, mineral density analysis of enamel and dentin revealed no significant differences between affected and normal teeth. The observations reported in this case suggest that resorptive changes, possibly influenced by inflammatory factors, could contribute to the observed root anomalies. The preservation of alveolar bone height provides a favourable prognosis for prosthetic rehabilitation.

Conclusion

These findings help elucidate the structural and functional implications of ROs. This study highlights the importance of early diagnosis and multidisciplinary management for optimal outcomes.

背景/目的局部牙发育不良（RO）是一种罕见的影响牙齿组织形成的发育异常，通常局限于单一象限。表现为牙釉质发育不全，牙本质不规则，根短尖开，影像学表现为“鬼影样”。其病因尚不清楚，血管，遗传和炎症因素提出。材料和方法在本研究中，我们报告了一名15岁的女性下颌RO影响犬齿，前磨牙和磨牙的病例，重点是对受影响牙齿的临床，x线摄影和显微镜分析，包括矿物质密度评估。结果第2前磨牙和第1、2磨牙（35、36、37牙）形态缺损严重，根极短（减少40%、90%、95%）拔除，但牙槽骨高度保持不变。犬齿和第一前磨牙受影响较小，按正畸治疗方案保留。扫描电镜显示，与对照组相比，患牙的牙本质小管数量和直径略有减少。与许多报道的RO病例相反，牙釉质和牙本质的矿物质密度分析显示，受影响的牙齿和正常牙齿之间没有显着差异。本病例报告的观察结果表明，可能受炎症因素影响的吸收变化可能导致观察到的根异常。保留牙槽骨高度为修复提供了良好的预后。结论这些发现有助于阐明活性氧的结构和功能意义。这项研究强调了早期诊断和多学科管理对最佳结果的重要性。

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引用次数: 0

A hybrid MMBERT framework for classifying periodontal bone loss: Integrating visual and textual information 一个用于牙周骨丢失分类的混合MMBERT框架：整合视觉和文本信息

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-14 DOI: 10.1016/j.jobcr.2025.12.013

Jennifer Jeyaruby, Pradeep Kumar Yadalam

Introduction

Periodontitis, a chronic inflammatory disease, causes bone loss and tooth instability. Early diagnosis, aided by clinical and radiographic assessments, is crucial. mmBERT, a multimodal deep learning approach, integrates image and text data to enhance bone loss prediction but faces challenges such as image variability and limited datasets. This study proposes a Hybrid mmBERT Framework to improve diagnostic accuracy in intraoral periapical (IOPA) radiographs.

Methods

A dataset of 150 IOPA images from Saveetha Dias includes expert-annotated interpretations of periodontal bone loss. Images are split into training and test sets. Clinical notes are preprocessed, normalized, and feature-extracted using ClinicalBERT. The mmBERT model fuses image and text embeddings into a 1024-dimensional space with a 0.3 dropout rate. Its architecture integrates a vision encoder (ResNet50), text encoder (ClinicalBERT), and a cross-modal transformer with 16 attention heads.

Results

The study shows strong overlap between predicted and ground-truth masks, good intersection-over-union performance, and acceptable boundary accuracy. The MMBERT model achieved final training and test accuracies of 99.11 % and 100 %, respectively, demonstrating its robustness and reliability in clinical applications.

Conclusion

The study presents a classification method for periodontal bone loss based on MMBERT architecture. It demonstrates promising radiographic interpretation performance while necessitating further clinical application research.

牙周炎是一种慢性炎症性疾病，会导致骨质流失和牙齿不稳定。在临床和放射学评估的帮助下进行早期诊断至关重要。mmBERT是一种多模态深度学习方法，它集成了图像和文本数据来增强骨质流失预测，但面临着图像可变性和数据集有限等挑战。本研究提出了一种混合mmBERT框架，以提高口腔内根尖周（IOPA） x线片的诊断准确性。方法来自Saveetha Dias的150张IOPA图像数据集包括专家注释的牙周骨质流失解释。图像分为训练集和测试集。使用ClinicalBERT对临床记录进行预处理、归一化和特征提取。mmBERT模型将图像和文本嵌入融合到1024维空间中，辍学率为0.3。它的架构集成了一个视觉编码器（ResNet50）、文本编码器（ClinicalBERT）和一个具有16个注意力头的跨模态转换器。结果预测掩模与真实掩模之间有较强的重叠，具有良好的交叉-超并性能，边界精度可接受。MMBERT模型的最终训练和测试准确率分别达到99.11%和100%，证明了其在临床应用中的鲁棒性和可靠性。结论提出了一种基于MMBERT结构的牙周骨丢失分类方法。它显示了良好的影像学解释性能，但需要进一步的临床应用研究。

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引用次数: 0

Comparison of ozone therapy and antimicrobial photodynamic therapy in periodontitis: A randomized clinical trial 臭氧治疗与抗菌光动力治疗牙周炎的比较：一项随机临床试验

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-13 DOI: 10.1016/j.jobcr.2025.12.011

Shilpi Mangal , Shalini Kaushal , Nand Lal , Anjani Kumar Pathak , Bhaskar Agarwal , Suruchi Shukla

Background

This randomized clinical trial evaluated the comparative efficacy of ozone therapy and ICG-mediated antimicrobial photodynamic therapy as adjunctive to scaling and root planing in the management of periodontitis, by assessing clinical outcomes, inflammatory biomarkers, specifically interleukin-1β (IL-1β) and Pentraxin-3 (PTX-3), and microbial load via quantitative PCR.

Methods

This is a parallel-arm, single-blinded, randomized controlled trial that included 36 patients with Stage III Grade B periodontitis, allocated randomly to three groups. Group 1 {Scaling and Root planing (SRP)}, Group 2 {SRP + Ozone Therapy (OT}), and Group 3 {SRP + Antimicrobial Photodynamic Therapy (aPDT)}. Clinical parameters, biochemical markers, and microbial count were recorded at baseline, 1 month, and 3 months.

Result

The change in Plaque Index (PI), Gingival Index (GI), Probing Pocket Depth (PPD), and Clinical Attachment Level (CAL) from baseline to 1 month and from baseline to 3 months was significantly higher in the SRP + OT group (p < 0.001). Reduction in the microbial count of Porphyromonas gingivalis (Keystone pathogen) (Mean difference = −0.70, p < 0.001) and Tannerella forsythia (−0.51, p < 0.001), whereas SRP + aPDT provided superior suppression for Fusobacterium nucleatum (−0.76, p < 0.001) and Prevotella intermedia (0.40, p < 0.001). No meaningful difference was observed between the two adjuncts for Treponema denticola (p = 0.994).

Conclusion

All treatment modalities resulted in significant improvements in all the parameters over time. However, SRP + Ozone Therapy consistently achieved the improvement across all parameters, when compared to SRP alone or SRP with aPDT.

本随机临床试验通过定量PCR评估临床结果、炎症生物标志物，特别是白细胞介素-1β (IL-1β)和ptraxin -3 (PTX-3)，以及微生物负荷，评估臭氧治疗和icg介导的抗菌光动力治疗在牙周炎治疗中的辅助效果。方法：这是一项平行、单盲、随机对照试验，包括36例III期B级牙周炎患者，随机分为三组。1组{除垢和刨根（SRP）}， 2组{SRP +臭氧治疗（OT）}， 3组{SRP +抗菌光动力治疗（aPDT）}。在基线、1个月和3个月记录临床参数、生化指标和微生物计数。结果SRP + OT组牙菌斑指数（PI）、牙龈指数（GI）、探查袋深度（PPD）、临床附着水平（CAL）的变化较对照组（p < 0.001）显著升高。减少了牙龈卟啉单胞菌（Keystone病原菌）（平均差值= - 0.70,p < 0.001）和连铸单宁菌（- 0.51,p < 0.001）的微生物数量，而SRP + aPDT对核梭杆菌（- 0.76,p < 0.001）和中间普氏菌（0.40,p < 0.001）具有较好的抑制作用。密螺旋体和齿状体的检测结果无显著性差异（p = 0.994）。结论随着时间的推移，所有治疗方式均能显著改善患者的各项指标。然而，与单独SRP或SRP + aPDT相比，SRP +臭氧疗法在所有参数上都得到了持续的改善。

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引用次数: 0

Synergistic effects of zinc oxide and ellagic acid on macrophage infiltration, angiogenesis, and collagen deposition in excisional wounds healing 氧化锌和鞣花酸对切除伤口愈合中巨噬细胞浸润、血管生成和胶原沉积的协同作用

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-13 DOI: 10.1016/j.jobcr.2026.01.002

Intan Nirwana , Michelle Fidelia Alexandra , Tarissa Balqis Nuraida , Ahmad Thariq Bermani , Yasmin Shahaniz , Devi Rianti , Titien Hary Agustantina , Anita Yuliati , Khairul Annuar Shariff , Meircurius Dwi Condro Surboyo

Objectives

The combination of zinc oxide (ZnO) and ellagic acid (EA) in wound dressings offers synergistic benefits for wound healing due to their complementary antimicrobial, antioxidant, anti-inflammatory, and tissue regenerative properties. This study aimed to evaluate the effects of ZnO-EA dressing on macrophage infiltration, angiogenesis, and collagen density in excisional wounds on days 3 and 7 post-treatment.

Materials and methods

This in vivo posttest-only experimental study involved twenty Rattus norvegicus with excisional dorsal wounds, randomly assigned to two groups: a control group receiving ZnO dressing and a treatment group receiving ZnO-EA dressing. Each group was evaluated at day 3 and day 7, resulting in four observation subgroups. Tissue samples were collected to assess macrophage infiltration and angiogenesis using Hematoxylin and Eosin staining, and collagen density using Masson's Trichrome staining. Histological evaluation was performed under a light microscope at 400× magnification.

Results

Treatment with ZnO-EA resulted in significant increase in macrophages infiltration, the number of newly formed blood vessels, and collagen density in the treatment group compared to the ZnO group on both days 3 and 7 (p < 0.05).

Conclusion

The application of a ZnO wound dressing enriched with EA significantly enhances macrophage infiltration, angiogenesis, and collagen deposition during the wound healing process.

目的将氧化锌（ZnO）和鞣花酸（EA）结合在伤口敷料中，具有抗菌、抗氧化、抗炎和组织再生的协同作用。本研究旨在评价ZnO-EA敷料在治疗后第3天和第7天对切除创面巨噬细胞浸润、血管生成和胶原蛋白密度的影响。材料与方法本实验选取20只背部切除创面的褐家鼠，随机分为两组：对照组和治疗组分别采用ZnO- ea敷料。每组在第3天和第7天进行评估，形成4个观察亚组。收集组织样本，用苏木精和伊红染色评估巨噬细胞浸润和血管生成，用马松三色染色评估胶原蛋白密度。光镜下400倍放大进行组织学观察。结果与ZnO组相比，ZnO- ea治疗组在第3天和第7天巨噬细胞浸润、新生血管数量和胶原蛋白密度均显著增加（p < 0.05）。结论添加EA的氧化锌创面敷料可显著增强创面愈合过程中巨噬细胞的浸润、血管生成和胶原的沉积。

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引用次数: 0

Evaluating the efficacy of eggshell-derived hydroxyapatite (EHA) and synthetic hydroxyapatite (SHA) in bone regeneration of maxillofacial defects-a randomized double-blind pilot comparative clinical-radiological study 评价蛋壳衍生羟基磷灰石（EHA）和合成羟基磷灰石（SHA）在颌面部缺损骨再生中的疗效——一项随机双盲先导比较临床放射学研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 DOI: 10.1016/j.jobcr.2025.11.016

Khushboo Changani , Anil Managutti , Shailesh Menat , Nirav Patel , Vivekanand Kattimani , Darshal Panchal , E.K. Girija

Background

Defects of the maxillofacial skeleton lead to functional, aesthetic, social, and behavioral problems, that make the person isolate from the mainstream of society. So, bone regeneration is needed for the overall health. Since long time various materials have been used for bone regeneration with limitations. Recently, Eggshell derived hydroxyapatite [EHA] has been explored as a graft substitute. Hence EHA is evaluated and compared with SHA (synthetic hydroxyapatite) for its efficacy to accelerate bone regeneration in maxillofacial cystic defects.

Materials and methods

Total of 20 bony defects randomly allocated to SHA and EHA groups for grafting after removal of pathology (cyst and granuloma) having ≤ 2 cm in size. Signs and symptoms were evaluated postsurgically for 2 weeks. Bone regeneration was assessed by blinded observer for study duration and method of intervention postoperatively at1^st, 2nd, 4th, and 6th month and 1 year.

Results

The study found no statistical significant difference among clinical signs and symptoms between the groups. Whereas EHA showed better bone regeneration by the end of 6th month and 1 year compared to SHA with statistically significant difference in bone density[EHA group (136.04 ± 15.56) and SHA group (115.58 ± 16.26)]. Parameters like internal portion of surgical site, surgical site outline showed consistent results with density.

Conclusion

Both EHA and SHA graft materials are equally efficient in early bone regeneration. The EHA showed promising results and enhanced bone regeneration with osteoconductivity, which indicates the eggshell waste-bio mineral is worthwhile raw material for the production of HA and is Go Green procedure. EHA is economic, compared with SHA.

颌面骨骼的缺陷导致功能、审美、社会和行为问题，使人与社会主流隔离。因此，骨骼再生是整体健康所必需的。长期以来，各种材料被用于骨再生，但都有局限性。近年来，蛋壳衍生的羟基磷灰石（EHA）作为移植物替代物被广泛研究。因此，我们评价EHA并与合成羟基磷灰石（SHA）比较其促进颌骨囊性缺损骨再生的效果。材料与方法取20例骨性缺损，切除病理（囊肿、肉芽肿），大小≤2cm，随机分为SHA组和EHA组进行移植。术后2周评估体征和症状。术后1、2、4、6个月和1年采用盲法观察骨再生情况，评估研究时间和干预方法。结果两组患者临床症状、体征差异无统计学意义。而EHA组在第6个月和第1年骨密度均优于SHA组，骨密度差异有统计学意义[EHA组（136.04±15.56）与SHA组（115.58±16.26）]。手术部位内部、手术部位轮廓等参数与密度一致。结论EHA和SHA移植材料在早期骨再生中的效果相同。结果表明，EHA具有良好的骨再生和骨导电性，表明蛋壳废物-生物矿物是一种有价值的生产HA的原料，是一种绿色工艺。与SHA相比，EHA更经济。

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引用次数: 0

Flourishing systematic reviews, but is evidence keeping pace? A critical evaluation and trend analysis of five orthodontic journals from 2015 to 2024 系统评价蓬勃发展，但证据跟上了吗？2015 - 2024年5家口腔正畸期刊批判性评价及趋势分析

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 DOI: 10.1016/j.jobcr.2025.12.008

J. Monisha, Balasubramanian Madhan

Introduction

Systematic reviews and meta-analyses (SRMAs) in Orthodontics have increased substantially, but whether this is commensurate with the evidence generated is questionable.

Objective

To evaluate the evidence outcome from SRMAs, the quality of included primary studies, and the proportion of SRMAs to original research in five orthodontic journals from 2015 to 2024.

Methods

SRMAs in Progress in Orthodontics (PO), Angle Orthodontist (AO), European Journal of Orthodontics (EJO), American Journal of Orthodontics and Dentofacial Orthopedics (AJODO), and Journal of the World Federation of Orthodontists (JWFO) published during this period were evaluated for the evidence outcome and Risk of Bias (RoB) of included studies. The proportion of SRMAs to original research was also calculated, and trends were compared across two five-year periods (2015–2019 and 2020–2024).

Results

During this period, 292 SRMAs evaluating 4511 studies for 707 outcomes were published. Of the evaluated outcomes, 16.7 % were fully conclusive, 69.2 % partially conclusive, and 14.1 % inconclusive. Most included studies (n = 3149) showed moderate (37.4 %) or high (32.3 %) RoB. Of all evaluated publications (n = 3904), 7.5 % were SRMAs. Some journals showed an increase in partially conclusive outcomes (AO, JWFO), the proportion of SRMA (EJO), and studies with high RoB, as well as a drop in fully conclusive outcomes (AO, AJODO, PO) in the latter half. However, overall differences remained statistically nonsignificant.

Conclusion

Though maintaining the proportion of SRMA to primary research by most journals is comforting, the SRMAs staying less productive in evidence, and primary research with high RoB are a matter of concern.

正畸学的系统综述和荟萃分析（srma）已大幅增加，但这是否与所产生的证据相称尚存疑问。目的评价2015 - 2024年5份口腔正畸期刊srma的证据结果、纳入的初步研究质量以及srma占原创研究的比例。方法对同期发表在《Progress in Orthodontics》（PO）、《Angle Orthodontist》（AO）、《European Journal of Orthodontics》（EJO）、《American Journal of orthotics and Dentofacial Orthopedics》（AJODO）和《Journal of World Federation of Orthodontists》（JWFO）上的ssrma进行纳入研究的证据结局和偏倚风险（Risk of Bias, RoB）评价。还计算了srma与原始研究的比例，并比较了两个五年期（2015-2019年和2020-2024年）的趋势。在此期间，共发表了292篇srma，评估了4511项研究的707个结果。在评估的结果中，16.7%是完全结论性的，69.2%是部分结论性的，14.1%是非结论性的。大多数纳入的研究（n = 3149）显示中度（37.4%）或高（32.3%）的RoB。在所有被评估的出版物（n = 3904）中，7.5%为srma。部分期刊显示部分结论性结果（AO、JWFO）、SRMA （EJO）和高RoB的研究比例增加，而完全结论性结果（AO、AJODO、PO）在后半段有所下降。然而，总体差异在统计学上仍然不显著。结论虽然大多数期刊保持SRMA在初级研究中的比例令人欣慰，但SRMA在证据方面的生产力仍然较低，初级研究的高RoB是值得关注的问题。

{"title":"Flourishing systematic reviews, but is evidence keeping pace? A critical evaluation and trend analysis of five orthodontic journals from 2015 to 2024","authors":"J. Monisha, Balasubramanian Madhan","doi":"10.1016/j.jobcr.2025.12.008","DOIUrl":"10.1016/j.jobcr.2025.12.008","url":null,"abstract":"<div><h3>Introduction</h3><div>Systematic reviews and meta-analyses (SRMAs) in Orthodontics have increased substantially, but whether this is commensurate with the evidence generated is questionable.</div></div><div><h3>Objective</h3><div>To evaluate the evidence outcome from SRMAs, the quality of included primary studies, and the proportion of SRMAs to original research in five orthodontic journals from 2015 to 2024.</div></div><div><h3>Methods</h3><div>SRMAs in Progress in Orthodontics (PO), Angle Orthodontist (AO), European Journal of Orthodontics (EJO), American Journal of Orthodontics and Dentofacial Orthopedics (AJODO), and Journal of the World Federation of Orthodontists (JWFO) published during this period were evaluated for the evidence outcome and Risk of Bias (RoB) of included studies. The proportion of SRMAs to original research was also calculated, and trends were compared across two five-year periods (2015–2019 and 2020–2024).</div></div><div><h3>Results</h3><div>During this period, 292 SRMAs evaluating 4511 studies for 707 outcomes were published. Of the evaluated outcomes, 16.7 % were fully conclusive, 69.2 % partially conclusive, and 14.1 % inconclusive. Most included studies (n = 3149) showed moderate (37.4 %) or high (32.3 %) RoB. Of all evaluated publications (n = 3904), 7.5 % were SRMAs. Some journals showed an increase in partially conclusive outcomes (AO, JWFO), the proportion of SRMA (EJO), and studies with high RoB, as well as a drop in fully conclusive outcomes (AO, AJODO, PO) in the latter half. However, overall differences remained statistically nonsignificant.</div></div><div><h3>Conclusion</h3><div>Though maintaining the proportion of SRMA to primary research by most journals is comforting, the SRMAs staying less productive in evidence, and primary research with high RoB are a matter of concern.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 267-272"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of bioactive extracellular soluble Hemarina (M101) derived scaffolds for neovascularization in periodontal tissue regeneration 生物活性细胞外可溶性海玛丽娜（M101）衍生支架在牙周组织再生中用于新生血管的评价

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 DOI: 10.1016/j.jobcr.2026.01.003

A. Mohamed Thaha, Kaarthikeyan Gurumoorthy

Background

Periodontitis leads to progressive destruction of periodontal tissues, where low oxygen levels and inadequate vascularization limit regenerative healing. Hemarina-101 (M101), a marine-sourced extracellular hemoglobin with high oxygen-binding and antioxidant potential, may overcome these barriers. This study investigated the ability of M101-incorporated chitosan–alginate scaffolds to support angiogenesis and cell compatibility for potential use in periodontal regeneration.

Methods

Chitosan–alginate scaffolds containing 10 % w/w M101 were fabricated and compared with plain scaffolds, untreated controls, and vascular endothelial growth factor (VEGF)-treated groups. Human periodontal ligament cells (PDLCs) and endothelial cells (EA.hy926) were cultured on the scaffolds. Cell viability and proliferation were analyzed using MTT assays and live/dead staining. Expression of angiogenic genes (VEGF-A, ANGPT1, CD31, HIF-1α) was quantified by qPCR. In vivo-like angiogenic responses were assessed using the chorioallantoic membrane (CAM) assay. Statistical significance was set at p < 0.05.

Results

Scaffolds with M101 showed significantly greater cell viability and proliferation than plain scaffolds (p < 0.05), comparable to VEGF-treated controls. Live/dead staining confirmed high densities of viable cells on M101 scaffolds. Gene expression analysis revealed notable upregulation of VEGF-A, ANGPT1, CD31, and HIF-1α in the M101 group, approaching levels seen with VEGF treatment. The CAM assay demonstrated dense, radially organized vessel networks forming around M101 scaffolds, indicating a strong pro-angiogenic effect.

Conclusion

M101-incorporated scaffolds enhanced endothelial cell growth, angiogenic gene activation, and neovascularization compared with plain scaffolds. These findings support the potential of M101-based biomaterials as promising candidates for periodontal tissue regeneration, meriting further preclinical and clinical validation.

牙周炎导致牙周组织的进行性破坏，其中低氧水平和血管化不足限制了再生愈合。Hemarina-101 （M101）是一种海洋来源的细胞外血红蛋白，具有高氧结合和抗氧化潜力，可以克服这些障碍。本研究探讨了m101掺入壳聚糖-海藻酸盐支架支持血管生成和细胞相容性的能力，为牙周再生提供潜在的应用前景。方法制备含有10% w/w M101的海藻酸壳聚糖支架，并与普通支架组、未处理组和血管内皮生长因子（VEGF）处理组进行比较。在支架上培养人牙周韧带细胞（pdlc）和内皮细胞（EA.hy926）。采用MTT法和活/死染色分析细胞活力和增殖。qPCR检测血管生成基因VEGF-A、ANGPT1、CD31、HIF-1α的表达。使用绒毛膜尿囊膜（CAM）试验评估体内样血管生成反应。p <； 0.05为统计学意义。结果M101支架的细胞活力和增殖能力明显高于普通支架（p < 0.05），与vegf处理的对照组相当。活/死染色证实M101支架上存在高密度活细胞。基因表达分析显示，M101组VEGF- a、ANGPT1、CD31和HIF-1α显著上调，接近VEGF治疗组的水平。CAM实验显示密集的、放射状组织的血管网络在M101支架周围形成，表明有很强的促血管生成作用。结论与普通支架相比，m101掺入支架可促进内皮细胞生长、血管生成基因激活和新生血管形成。这些发现支持了基于m101的生物材料作为牙周组织再生的有希望的候选材料的潜力，值得进一步的临床前和临床验证。

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引用次数: 0