Journal of oral biology and craniofacial research最新文献_第2页

Use of colorimetry as a diagnostic tool for early detection of peri-implant diseases 使用比色法作为早期检测种植体周围疾病的诊断工具

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-03-01 Epub Date: 2026-01-20 DOI: 10.1016/j.jobcr.2026.101406

Sergi Torné-Durán

Introduction

The increasing aesthetic demand in dentistry and the limitations of visual colour assessment have encouraged the development of objective methods for evaluating dental and peri-implant tissues. Although colourimeters were originally designed for tooth shade matching, their application has recently expanded to peri-implant soft-tissue analysis, providing quantitative and reproducible measurements capable of detecting early inflammatory changes.

Objectives

This study aimed to determine whether colour analysis using a colourimeter is a valid diagnostic tool for the preliminary detection of peri-implant diseases by comparing colourimetric data with conventional clinical findings.

Materials and methods

A cross-sectional, descriptive, and experimental study was conducted on 63 dental implants. Peri-implant soft-tissue colour was recorded using a colourimeter based on CIELab parameters (L∗, a∗, b∗). Each implant also underwent clinical evaluation including visual inspection, periodontal probing, and periapical radiography. Two measurement points were analysed: Point A, located 2 mm apical to the gingival margin, and Point B, positioned beyond the probing depth for each implant. Colourimetric values from both points were compared with the clinical diagnosis obtained for every implant.

Results

At Point B, peri-implant tissues showed lower luminosity (L∗), higher redness (a∗), and reduced b∗ values compared with Point A. Clinically, 48 implants presented peri-implantitis, 9 mucositis, and 6 were considered healthy. Diseased implants demonstrated darker and more reddish peri-implant tissues, revealing a clear correlation between colour alterations and inflammatory status.

牙科审美需求的增加和视觉颜色评估的局限性鼓励了评估牙齿和种植体周围组织的客观方法的发展。虽然色度计最初是为牙齿色度匹配而设计的，但它们的应用最近已扩展到种植体周围软组织分析，提供能够检测早期炎症变化的定量和可重复测量。目的本研究旨在通过比较比色仪的数据与常规临床表现，确定颜色分析是否是一种有效的诊断工具，用于初步检测种植体周围疾病。材料与方法对63例种植体进行了横断面、描述性和实验性研究。采用基于CIELab参数（L∗,a∗,b∗）的色度计记录种植体周围软组织颜色。每个种植体还进行了临床评估，包括目视检查、牙周探查和根尖周x线摄影。分析了两个测量点：A点位于龈缘顶部2mm处，B点位于每个种植体的探测深度之外。将两个点的比色值与每个种植体的临床诊断结果进行比较。结果B点种植体周围组织亮度（L∗）较a点低，红度（a∗）较高，B∗值降低。临床表现为种植体周围炎48例，黏膜炎9例，健康6例。病变种植体表现为种植体周围组织颜色更深、更红，表明颜色改变与炎症状态之间存在明确的相关性。

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引用次数: 0

A hybrid MMBERT framework for classifying periodontal bone loss: Integrating visual and textual information 一个用于牙周骨丢失分类的混合MMBERT框架：整合视觉和文本信息

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-03-01 Epub Date: 2026-01-14 DOI: 10.1016/j.jobcr.2025.12.013

Jennifer Jeyaruby, Pradeep Kumar Yadalam

Introduction

Periodontitis, a chronic inflammatory disease, causes bone loss and tooth instability. Early diagnosis, aided by clinical and radiographic assessments, is crucial. mmBERT, a multimodal deep learning approach, integrates image and text data to enhance bone loss prediction but faces challenges such as image variability and limited datasets. This study proposes a Hybrid mmBERT Framework to improve diagnostic accuracy in intraoral periapical (IOPA) radiographs.

Methods

A dataset of 150 IOPA images from Saveetha Dias includes expert-annotated interpretations of periodontal bone loss. Images are split into training and test sets. Clinical notes are preprocessed, normalized, and feature-extracted using ClinicalBERT. The mmBERT model fuses image and text embeddings into a 1024-dimensional space with a 0.3 dropout rate. Its architecture integrates a vision encoder (ResNet50), text encoder (ClinicalBERT), and a cross-modal transformer with 16 attention heads.

Results

The study shows strong overlap between predicted and ground-truth masks, good intersection-over-union performance, and acceptable boundary accuracy. The MMBERT model achieved final training and test accuracies of 99.11 % and 100 %, respectively, demonstrating its robustness and reliability in clinical applications.

Conclusion

The study presents a classification method for periodontal bone loss based on MMBERT architecture. It demonstrates promising radiographic interpretation performance while necessitating further clinical application research.

牙周炎是一种慢性炎症性疾病，会导致骨质流失和牙齿不稳定。在临床和放射学评估的帮助下进行早期诊断至关重要。mmBERT是一种多模态深度学习方法，它集成了图像和文本数据来增强骨质流失预测，但面临着图像可变性和数据集有限等挑战。本研究提出了一种混合mmBERT框架，以提高口腔内根尖周（IOPA） x线片的诊断准确性。方法来自Saveetha Dias的150张IOPA图像数据集包括专家注释的牙周骨质流失解释。图像分为训练集和测试集。使用ClinicalBERT对临床记录进行预处理、归一化和特征提取。mmBERT模型将图像和文本嵌入融合到1024维空间中，辍学率为0.3。它的架构集成了一个视觉编码器（ResNet50）、文本编码器（ClinicalBERT）和一个具有16个注意力头的跨模态转换器。结果预测掩模与真实掩模之间有较强的重叠，具有良好的交叉-超并性能，边界精度可接受。MMBERT模型的最终训练和测试准确率分别达到99.11%和100%，证明了其在临床应用中的鲁棒性和可靠性。结论提出了一种基于MMBERT结构的牙周骨丢失分类方法。它显示了良好的影像学解释性能，但需要进一步的临床应用研究。

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引用次数: 0

Compressive and flexural strength of provisional crown materials fabricated by conventional and 3D printing techniques: An in vitro study 传统和3D打印技术制备的临时冠材料的抗压和弯曲强度：体外研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-03-01 Epub Date: 2026-01-27 DOI: 10.1016/j.jobcr.2026.101405

Nandini R.D., Nirupama R, Chetan Hegde

Purpose

To compare the mechanical properties of the new 3D Accuprint C&B resin against both conventional and other 3D-printed resins to determine its viability for provisional crown fabrication.

Materials and methods

Three materials were selected for comparison: GC Tempron (conventional), 3D Accuprint C&B, and Freeprint Temp (3D-printed resins). Fifteen cylindrical specimens (6 mm × 4 mm) were prepared from each material for compressive strength testing, and fifteen rectangular specimens (25 mm × 2 mm × 2 mm) were fabricated for flexural strength testing as per the manufacturer's instructions. Testing was performed using a universal testing machine. The recorded compressive and flexural strength values were statistically analyzed after confirming normality (Shapiro–Wilk test) and homogeneity (Levene's test). All statistical analyses were conducted using IBM SPSS Statistics software (version 25), with the level of significance set at p < 0.05. Data were analyzed using one-way ANOVA with Tukey's HSD test.

Results

Significant intergroup differences were found for both compressive and flexural strength (p < 0.001). GC Tempron showed significantly lower values compared with both 3D printed materials (p < 0.001). Freeprint Temp and 3D Accuprint did not differ significantly (compressive strength, p = 0.84; flexural strength, p = 0.15).

Conclusions

3D Accuprint C&B demonstrated superior mechanical strength compared to GC Tempron and comparable properties to Freeprint Temp. These findings confirm its mechanical viability as a high-performance alternative, making it better suited for durable provisional restorations, particularly in cases involving high resistance to stress, parafunctional habits, or implant-supported prostheses.

目的将新型3D Accuprint C&；B树脂与传统树脂和其他3D打印树脂的机械性能进行比较，以确定其用于临时冠制造的可行性。材料和方法选择三种材料进行比较：GC Tempron（常规）、3D Accuprint C&；B和Freeprint Temp （3D打印树脂）。每种材料制备15个圆柱形试件（6mm × 4mm）进行抗压强度试验，15个矩形试件（25mm × 2mm × 2mm）进行抗折强度试验。使用通用试验机进行测试。经证实正态性（Shapiro-Wilk检验）和均匀性（Levene检验）后，对记录的抗压和抗弯强度值进行统计学分析。采用IBM SPSS统计软件（version 25）进行统计分析，显著性水平设置为p <； 0.05。数据分析采用Tukey’s HSD检验的单因素方差分析。结果抗压和抗弯强度组间差异显著（p < 0.001）。与两种3D打印材料相比，GC Tempron的数值显著降低（p < 0.001）。Freeprint Temp和3D Accuprint没有显著差异（抗压强度，p = 0.84；抗弯强度，p = 0.15）。与GC Tempron相比，3d Accuprint C&；B具有更高的机械强度，与Freeprint Temp相比具有类似的性能。这些研究结果证实了其作为高性能替代材料的机械可行性，使其更适合于持久的临时修复，特别是在具有高抗应力性、功能习惯或种植体支持的情况下。

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引用次数: 0

High UBE2D1 expression is associated with poor prognosis and immunotherapy resistance in head and neck cancer UBE2D1高表达与头颈癌预后不良和免疫治疗耐药相关。

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-03-01 Epub Date: 2026-02-14 DOI: 10.1016/j.jobcr.2026.101423

Prathibha Prasad , Paramasivam Arumugam

Background

Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy with poor clinical outcomes. Ubiquitin-conjugating enzyme E2 D1 (UBE2D1), a key component of the ubiquitination machinery, has been implicated in tumor progression in several cancers; however, its relevance in HNSCC remains unclear. This study aimed to investigate UBE2D1 expression in HNSCC, evaluate its prognostic significance while acknowledging disease heterogeneity, including HPV and TP53 status, and explore its potential association with response to anti-PD-1 immunotherapy.

Methods

UBE2D1 mRNA and protein expression were analyzed using the Cancer Genome Atlas Head and Neck Squamous Cell Carcinoma (TCGA-HNSCC) dataset via the UALCAN platform. Transcript-level expression was validated by quantitative real-time PCR in paired HNSCC and adjacent normal tissues. Protein–protein interaction and functional enrichment analyses were performed to examine UBE2D1-associated molecular networks. Prognostic relevance was assessed using Kaplan–Meier survival analysis.

Results

UBE2D1 mRNA and protein expression were significantly elevated in HNSCC compared with normal tissues (P < 0.05). Higher UBE2D1 expression was associated with poorer overall and relapse-free survival. Exploratory analyses suggested a weak association between elevated UBE2D1 expression and response patterns to anti-PD-1 therapy. Functional analyses identified associations with oncogenic pathways, with UBE2D1 expression positively correlated with MMP2 and negatively correlated with SMAD4, indicating a potential role in tumor progression.

Conclusion

UBE2D1 is frequently overexpressed in HNSCC and is associated with adverse clinical outcomes. Although its association with immunotherapy response requires further validation, UBE2D1 may be a prognostic biomarker in HNSCC.

背景：头颈部鳞状细胞癌（HNSCC）是一种侵袭性恶性肿瘤，临床预后较差。泛素结合酶E2D1 （UBE2D1）是泛素化机制的关键组成部分，与几种癌症的肿瘤进展有关；然而，其在HNSCC中的相关性尚不清楚。本研究旨在研究UBE2D1在HNSCC中的表达，评估其预后意义，同时承认疾病异质性，包括HPV和TP53状态，并探讨其与抗pd -1免疫治疗反应的潜在关联。方法：通过UALCAN平台，使用Cancer Genome Atlas Head and Neck Squamous Cell Carcinoma （TCGA-HNSCC）数据集分析UBE2D1 mRNA和蛋白表达。通过实时定量PCR在配对的HNSCC和邻近正常组织中验证转录水平表达。通过蛋白相互作用和功能富集分析来检测ube2d1相关的分子网络。使用Kaplan-Meier生存分析评估预后相关性。结果：与正常组织相比，UBE2D1 mRNA和蛋白表达在HNSCC中显著升高(P结论：UBE2D1在HNSCC中经常过表达，并与不良临床结局相关。尽管UBE2D1与免疫治疗反应的关联有待进一步验证，但它可能是HNSCC的预后生物标志物。

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引用次数: 0

Effect of surface preparation methods on the shear bond strength of universal and self-etch adhesive systems: an in vitro study 表面处理方法对通用和自蚀刻胶粘剂系统剪切结合强度的影响：体外研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-03-01 Epub Date: 2026-01-19 DOI: 10.1016/j.jobcr.2026.101408

Shiwangi Verma , Ravishankar Suman , Rajendra Goud , Snigdho Das , Srikant Gollapudi , Samapika Routray

Objectives

To assess the impact of three smear layer preparation methods on shear bond strength (SBS) to enamel and dentin, using two universal adhesives (Tetric N-Bond Universal, 3M Single Bond Universal) and one two-step self-etch adhesive (Kuraray Clearfil SE Bond).

Methods

Ninety extracted premolars were assigned to enamel and dentin groups (n = 45 each) and prepared with coarse grit (120), fine grit (600) silicon carbide paper, or air abrasion (Aluminium Oxide for enamel, Sylc for dentin). Surfaces were bonded with one of the adhesives and restored with composite. Enamel was treated in an etch-and-rinse mode, dentin in self-etch mode. SBS was tested using a universal testing machine. Failure surfaces were examined under SEM to assess fracture morphology and EDS to determine elemental composition. Data were analyzed using two-way ANOVA/Tukey's post-hoc test and Weibull statistics (P ≤ 0.05).

Results

In enamel, SBS generally increased from coarse to fine to air abrasion. Kuraray Clearfil SE Bond yielded the highest values, particularly with air abrasion (9.11 ± 0.51 MPa). In dentin, fine grit achieved the highest SBS across all adhesives, followed by air abrasion; coarse grit showed the lowest. Kuraray consistently outperformed the universal adhesives in most dentin conditions. SEM/EDS revealed predominantly adhesive failures; occasional cohesive failures in dentin (Ivoclar) and enamel (Kuraray) corresponded with higher SBS and Weibull reliability.

Conclusion

Both surface preparation and adhesive type significantly influence SBS. Kuraray showed superior performance and reliability, while fine grit and air abrasion enhanced bonding efficacy. SEM/EDS findings corroborated the mechanical results.

目的采用两种通用胶粘剂（Tetric N-Bond universal、3M Single bond universal）和一种两步自蚀刻胶粘剂（Kuraray Clearfil SE bond），评价三种涂抹层制备方法对牙釉质和牙本质剪切结合强度（SBS）的影响。方法将90颗拔除的前磨牙分为牙本质组和牙釉质组，每组45颗，分别用粗磨（120颗）、细磨（600颗）碳化硅纸或空气磨蚀（氧化铝磨蚀牙釉质，硫胺磨蚀牙本质）进行磨蚀。用其中一种粘合剂粘合表面，并用复合材料修复。牙釉质采用蚀刻-冲洗模式，牙本质采用自蚀刻模式。使用通用试验机对SBS进行了测试。在扫描电镜和能谱下对断裂表面进行了检查，以评估断裂形态和确定元素组成。数据分析采用双因素方差分析/Tukey事后检验和威布尔统计（P≤0.05）。结果在牙釉质中，SBS由粗到细再到气蚀逐渐增加。Kuraray Clearfil SE Bond获得了最高的值，特别是在空气磨损时（9.11±0.51 MPa）。在牙本质中，细砂在所有粘合剂中SBS最高，其次是空气磨损；粗粒度最低。在大多数牙本质条件下，Kuraray始终优于通用粘接剂。SEM/EDS主要显示胶粘剂失效；牙本质（Ivoclar）和牙釉质（Kuraray）偶尔的内聚失败与较高的SBS和Weibull可靠性相对应。结论表面处理和黏合剂类型对SBS均有显著影响。Kuraray表现出优异的性能和可靠性，而细粒度和空气磨损则增强了粘接效果。SEM/EDS结果证实了力学结果。

{"title":"Effect of surface preparation methods on the shear bond strength of universal and self-etch adhesive systems: an in vitro study","authors":"Shiwangi Verma , Ravishankar Suman , Rajendra Goud , Snigdho Das , Srikant Gollapudi , Samapika Routray","doi":"10.1016/j.jobcr.2026.101408","DOIUrl":"10.1016/j.jobcr.2026.101408","url":null,"abstract":"<div><h3>Objectives</h3><div>To assess the impact of three smear layer preparation methods on shear bond strength (SBS) to enamel and dentin, using two universal adhesives (Tetric N-Bond Universal, 3M Single Bond Universal) and one two-step self-etch adhesive (Kuraray Clearfil SE Bond).</div></div><div><h3>Methods</h3><div>Ninety extracted premolars were assigned to enamel and dentin groups (n = 45 each) and prepared with coarse grit (120), fine grit (600) silicon carbide paper, or air abrasion (Aluminium Oxide for enamel, Sylc for dentin). Surfaces were bonded with one of the adhesives and restored with composite. Enamel was treated in an etch-and-rinse mode, dentin in self-etch mode. SBS was tested using a universal testing machine. Failure surfaces were examined under SEM to assess fracture morphology and EDS to determine elemental composition. Data were analyzed using two-way ANOVA/Tukey's post-hoc test and Weibull statistics (P ≤ 0.05).</div></div><div><h3>Results</h3><div>In enamel, SBS generally increased from coarse to fine to air abrasion. Kuraray Clearfil SE Bond yielded the highest values, particularly with air abrasion (9.11 ± 0.51 MPa). In dentin, fine grit achieved the highest SBS across all adhesives, followed by air abrasion; coarse grit showed the lowest. Kuraray consistently outperformed the universal adhesives in most dentin conditions. SEM/EDS revealed predominantly adhesive failures; occasional cohesive failures in dentin (Ivoclar) and enamel (Kuraray) corresponded with higher SBS and Weibull reliability.</div></div><div><h3>Conclusion</h3><div>Both surface preparation and adhesive type significantly influence SBS. Kuraray showed superior performance and reliability, while fine grit and air abrasion enhanced bonding efficacy. SEM/EDS findings corroborated the mechanical results.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101408"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regional odontodysplasia: a report of unusual case with in-depth analysis 局部牙发育不良：罕见病例报告并深入分析

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-03-01 Epub Date: 2026-01-15 DOI: 10.1016/j.jobcr.2025.12.012

Charles Béhot , Ophélie Cuisinier , Éden Serraf , Lisa Friedlander , Brigitte Vi-Fane , Pascal Garrec , Ali Nassif

Background/purpose

Regional odontodysplasia (RO) is a rare developmental anomaly affecting dental tissue formation, typically confined to a single quadrant. It manifests through hypoplastic enamel, irregular dentin, short roots with open apices, and a characteristic “ghost-like” radiographic appearance. Its etiology remains unclear, with vascular, genetic and inflammatory factors proposed.

Materials and methods

In this study, we report the case of a 15-year-old female with mandibular RO affecting canine, premolars, and molars, focusing on the clinical, radiographic, and microscopic analysis of the affected teeth, including mineral density evaluation.

Results

The 2nd premolar and first and second molars (teeth 35, 36, 37) exhibited severe morphological defects and were extracted due to extreme root shortening (40 %, 90 %, and >95 % reduction), while alveolar bone height remained preserved. The canine and 1st premolar were affected to a lesser extent and were conserved upon orthodontic treatment plan. Scanning electron microscopy revealed slight decreases in the number and diameter of the dentinal tubules in the affected teeth compared with those in the control teeth. Contrary to many reported cases of RO, mineral density analysis of enamel and dentin revealed no significant differences between affected and normal teeth. The observations reported in this case suggest that resorptive changes, possibly influenced by inflammatory factors, could contribute to the observed root anomalies. The preservation of alveolar bone height provides a favourable prognosis for prosthetic rehabilitation.

Conclusion

These findings help elucidate the structural and functional implications of ROs. This study highlights the importance of early diagnosis and multidisciplinary management for optimal outcomes.

背景/目的局部牙发育不良（RO）是一种罕见的影响牙齿组织形成的发育异常，通常局限于单一象限。表现为牙釉质发育不全，牙本质不规则，根短尖开，影像学表现为“鬼影样”。其病因尚不清楚，血管，遗传和炎症因素提出。材料和方法在本研究中，我们报告了一名15岁的女性下颌RO影响犬齿，前磨牙和磨牙的病例，重点是对受影响牙齿的临床，x线摄影和显微镜分析，包括矿物质密度评估。结果第2前磨牙和第1、2磨牙（35、36、37牙）形态缺损严重，根极短（减少40%、90%、95%）拔除，但牙槽骨高度保持不变。犬齿和第一前磨牙受影响较小，按正畸治疗方案保留。扫描电镜显示，与对照组相比，患牙的牙本质小管数量和直径略有减少。与许多报道的RO病例相反，牙釉质和牙本质的矿物质密度分析显示，受影响的牙齿和正常牙齿之间没有显着差异。本病例报告的观察结果表明，可能受炎症因素影响的吸收变化可能导致观察到的根异常。保留牙槽骨高度为修复提供了良好的预后。结论这些发现有助于阐明活性氧的结构和功能意义。这项研究强调了早期诊断和多学科管理对最佳结果的重要性。

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引用次数: 0

Effect of dimethyl sulfoxide pretreatment on bonding of universal adhesives to pulp chamber dentin 二甲亚砜预处理对通用胶粘剂与牙本质粘接的影响

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-03-01 Epub Date: 2026-01-29 DOI: 10.1016/j.jobcr.2026.101409

Yashmin Parveen Karishma, Rajni Nagpal, Udai Pratap Singh, Sakshi Saumya, Shivani Deshwal

Aim and objective

To evaluate the effect of 50 % (v/v) dimethyl sulfoxide (DMSO) pretreatment on immediate (24 h) and delayed (6 months) microtensile bond strength (μTBS) of an etch-and-rinse adhesive and two universal adhesives to dentin.

Materials and methods

A total of 144 extracted human molars were decoronated, pulp chambers exposed, and roots sectioned 2 mm apical to the CEJ. Teeth were randomly divided into six experimental groups (n = 24), based on surface pretreatment and adhesive system used: Group 1A: Adper Single Bond 2 (ASB) Group 2A: Tetric N-Bond Universal (TNBU) Group 3A: Prime & Bond Universal (PNBU) Groups 1B, 2B, and 3B received the same adhesives, respectively, following a 50 % (v/v) DMSO wet bonding pretreatment. All adhesives were applied per manufacturer's instructions, followed by composite resin build-up. Ten samples per group were tested for μTBS at 24 h and ten after 6 months of storage. Failure mode was analyzed. Four additional samples per group underwent SEM evaluation to observe the resin–dentin interface.

Statistical analysis

Data were analyzed using one-way ANOVA and Tukey's post hoc test for intergroup comparisons. Unpaired t-tests were applied for two-group comparisons. Significance was set at p < 0.05.

Results

DMSO pretreatment significantly improved both immediate and long-term μTBS (p < 0.05) across all adhesives. PNBU exhibited the highest bond strength at both time intervals.

Conclusion

Dentin pretreatment with 50 % DMSO prior to adhesive application enhances immediate μTBS and preserves bond durability at 6 months, regardless of adhesive system used.

目的评价50% （v/v）二甲亚砜（DMSO）预处理对一种蚀刻冲洗胶粘剂和两种通用胶粘剂对牙本质的即时（24 h）和延迟（6个月）微拉伸结合强度（μTBS）的影响。材料和方法对144颗拔除的人磨牙进行装饰，露出牙髓腔，将牙根切成2 mm至CEJ。根据牙体表面处理和使用的粘结剂体系，随机分为6组（n = 24）： 1A组：Adper Single Bond 2 (ASB)， 2A组：tritric n -Bond Universal (TNBU)， 3A组：Prime &； Bond Universal (PNBU)， 1B、2B、3B组分别采用相同的粘结剂，采用50% (v/v) DMSO湿粘接预处理。所有胶粘剂都按照制造商的说明使用，然后是复合树脂堆积。贮藏24 h时每组10个样品，贮藏6个月后每组10个样品进行μTBS检测。分析了失效模式。每组增加4个样品进行扫描电镜观察树脂-牙本质界面。统计分析数据采用单因素方差分析和Tukey事后检验进行组间比较。两组比较采用非配对t检验。p <； 0.05为显著性。结果dmso预处理显著提高了所有胶粘剂的近期和长期μTBS （p < 0.05）。PNBU在两个时间间隔内均表现出最高的结合强度。结论无论采用何种粘接剂体系，使用50% DMSO预处理牙本质均可提高即刻μTBS，并保持6个月后粘接剂的耐久性。

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引用次数: 0

Differential effect of Periodontal pathogen Fusobacterium nucleatum and Porphyromonas gingivalis on modulation of specific partial-EMT genes in colorectal cancer cells 牙周病原体核梭杆菌和牙龈卟啉单胞菌对结直肠癌细胞特定部分emt基因调节的差异影响。

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-03-01 Epub Date: 2026-02-06 DOI: 10.1016/j.jobcr.2026.101410

Divya Gopinath , Balachandar Selvakumar , Priyadharshini Sekar , Marwan Mansoor Mohammed , Zhengrui Li

Objective

While the epithelial-mesenchymal transition (EMT) is known to promote cancer stemness and metastasis, a hybrid partial EMT (p-EMT) state has recently been identified. This study examined the influence of Fusobacterium nucleatum (Fn) and Porphyromonas gingivalis (Pg) on p-EMT gene expression and their prognostic relevance in colorectal cancer (CRC).

Materials and methods

HCT 116 cells were infected with Fn or Pg at an MOI of 100. Expression of p-EMT markers (SPP1, EMP3, INHBA, THBS2, ITGA5, P4HA2) and EMT-markers (ZEB1, SNAI1) was analyzed via RT-PCR after 6 hours (h) and 24 h. Cell migration was assessed using a scratch assay. TCGA data were used to validate gene expression in CRC patients, and Kaplan-Meier analysis was used to evaluate prognostic significance.

Results

Pg infection induced a significant upregulation of EMP3 at the 24 h, while suppressing the expression of P4HA2, MXD1, and THBS2. The ZEB2 displayed a biphasic response, decreasing at 6 h before rising at 24 h, and SNAI1 was consistently suppressed. In contrast, Fn. infection resulted in the upregulation of ITGA5 and MMP3, accompanied by the downregulation of P4HA2, MXD1, MMP9, and SNAI1. Both enhanced cellular migration and wound closure capabilities. THBS2, CDH13, and P4HA2 were significantly elevated in tumor tissues compared to controls, while EMP3 and ZEB1 showed reduced expression. Elevated mRNA levels of EMP3, THBS2, and ITGA5 were significantly correlated with poorer overall survival.

Conclusion

Fn and Pg can promote invasive phenotypes in CRC through distinct mechanisms, each modulating a unique subset of p-EMT and without instigating a complete, coordinated EMT gene signature.

目的：虽然已知上皮-间质转化（EMT）可促进癌症的发生和转移，但最近发现了一种混合部分EMT （p-EMT）状态。本研究探讨了核梭菌（Fn）和牙龈卟啉单胞菌（Pg）对结直肠癌（CRC） p-EMT基因表达的影响及其与预后的相关性。材料和方法：在MOI为100的条件下，用Fn或Pg感染HCT 116细胞。6小时(h)和24小时后，通过RT-PCR分析p-EMT标记物（SPP1、EMP3、INHBA、THBS2、ITGA5、P4HA2）和emt标记物（ZEB1、SNAI1）的表达。TCGA数据用于验证CRC患者的基因表达，Kaplan-Meier分析用于评估预后意义。结果：Pg感染后24 h EMP3表达明显上调，P4HA2、MXD1、THBS2表达明显抑制。ZEB2呈双相反应，6 h下降，24 h上升，SNAI1持续被抑制。相反，Fn。感染导致ITGA5、MMP3表达上调，P4HA2、MXD1、MMP9、SNAI1表达下调。增强细胞迁移和伤口愈合能力。与对照组相比，THBS2、CDH13、P4HA2在肿瘤组织中的表达显著升高，而EMP3、ZEB1的表达降低。EMP3、THBS2和ITGA5 mRNA水平升高与总生存率降低显著相关。结论：Fn和Pg可以通过不同的机制促进结直肠癌的侵袭性表型，各自调节p-EMT的一个独特子集，而不激发一个完整的、协调的EMT基因特征。

{"title":"Differential effect of Periodontal pathogen Fusobacterium nucleatum and Porphyromonas gingivalis on modulation of specific partial-EMT genes in colorectal cancer cells","authors":"Divya Gopinath , Balachandar Selvakumar , Priyadharshini Sekar , Marwan Mansoor Mohammed , Zhengrui Li","doi":"10.1016/j.jobcr.2026.101410","DOIUrl":"10.1016/j.jobcr.2026.101410","url":null,"abstract":"<div><h3>Objective</h3><div>While the epithelial-mesenchymal transition (EMT) is known to promote cancer stemness and metastasis, a hybrid partial EMT (p-EMT) state has recently been identified. This study examined the influence of <em>Fusobacterium nucleatum</em> (Fn) and <em>Porphyromonas gingivalis</em> (Pg) on p-EMT gene expression and their prognostic relevance in colorectal cancer (CRC).</div></div><div><h3>Materials and methods</h3><div>HCT 116 cells were infected with <em>Fn</em> or <em>Pg</em> at an MOI of 100. Expression of p-EMT markers (SPP1, EMP3, INHBA, THBS2, ITGA5, P4HA2) and EMT-markers (ZEB1, SNAI1) was analyzed via RT-PCR after 6 hours (h) and 24 h. Cell migration was assessed using a scratch assay. TCGA data were used to validate gene expression in CRC patients, and Kaplan-Meier analysis was used to evaluate prognostic significance.</div></div><div><h3>Results</h3><div><em>Pg</em> infection induced a significant upregulation of EMP3 at the 24 h, while suppressing the expression of P4HA2, MXD1, and THBS2. The ZEB2 displayed a biphasic response, decreasing at 6 h before rising at 24 h, and SNAI1 was consistently suppressed. In contrast, <em>Fn.</em> infection resulted in the upregulation of ITGA5 and MMP3, accompanied by the downregulation of P4HA2, MXD1, MMP9, and SNAI1. Both enhanced cellular migration and wound closure capabilities. THBS2, CDH13, and P4HA2 were significantly elevated in tumor tissues compared to controls, while EMP3 and ZEB1 showed reduced expression. Elevated mRNA levels of EMP3, THBS2, and ITGA5 were significantly correlated with poorer overall survival.</div></div><div><h3>Conclusion</h3><div><em>Fn</em> and <em>Pg</em> can promote invasive phenotypes in CRC through distinct mechanisms, each modulating a unique subset of p-EMT and without instigating a complete, coordinated EMT gene signature.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101410"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finite element biomechanical comparison of bone-driven and occlusion-driven fibular flap positioning in lateral segmental mandibular reconstruction 骨驱动与封闭驱动腓骨瓣定位在下颌外侧节段重建中的有限元生物力学比较。

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-03-01 Epub Date: 2026-02-05 DOI: 10.1016/j.jobcr.2026.101419

Dmytro Filonenko , Yurii Chepurnyi , Mykola Kryshchuk , Bekir Osmanov , Andrii Kopchak

Objective

To evaluate, using finite-element analysis (FEA), the stress–strain state of mandibular bone and the bone component of a microvascular free fibula flap (FFF) reconstructing a lateral segmental mandibular defect, comparing two flap positions relevant to implant-supported fixed prosthetic rehabilitation.

Materials and methods

CT-derived three-dimensional models of a reconstructed mandible were generated in two geometries: (A) flap aligned with the inferior mandibular border and (B) flap positioned at the level of the alveolar crest. Each geometry received two endosseous implants and a fixed prosthesis and was subjected to representative masticatory loading (vertical/occlusal and anterior/incisal). Four FEA models (2 positions × 2 load cases) were analyzed, with two predefined regions of interest per model (distal and medial), each examined at the upper and lower margins.

Results

Compared with Model A (inferior mandibular border), Model B (alveolar crest) produced lower peak bone stresses in most analyzed regions (≈10–40% reduction) under mastication-representative static molar and incisal loads. The only exception was the medial–inferior margin under incisal loading, where stress increased markedly (8.96 vs 2.22 MPa; ∼4-fold). Under occlusal loading, medial–inferior stresses were <1 MPa in both models. Across both positioning scenarios, peak crestal bone von Mises stresses clustered near the implant neck (crestal bone); analyses were restricted to bone.

Conclusion

A higher (alveolar-crest) position of the bone component of the FFF yields a more favorable stress–strain environment and load distribution, supporting more favorable biomechanical conditions for osseointegration and long-term function.

目的：应用有限元分析（FEA）评价游离腓骨微血管瓣（FFF）重建下颌骨外侧节段缺损的应力-应变状态及骨组分，比较两种游离腓骨瓣位置与种植体支撑固定假体康复的关系。材料和方法：重建下颌骨的ct三维模型以两种几何形状生成：(a)与下颌骨缘对齐的皮瓣和(B)位于牙槽嵴水平的皮瓣。每个几何形状的患者接受了两个骨内种植体和一个固定假体，并接受了代表性的咀嚼负荷（垂直/咬合和前牙/切牙）。分析了四个有限元模型（2个位置× 2个载荷情况），每个模型有两个预定义的感兴趣区域（远端和内侧），每个模型在上边缘和下边缘进行检查。结果：与A模型（下颌骨边界）相比，B模型（牙槽嵴）在具有咀嚼代表性的静态磨牙和切牙负荷下，在大多数分析区域产生的峰值骨应力较低（≈10-40%）。唯一的例外是在切牙负荷下的中下缘，应力显著增加（8.96 vs 2.22 MPa；约4倍）。结论：FFF骨组件较高的位置（牙槽嵴）产生更有利的应力-应变环境和载荷分布，为骨整合和长期功能提供更有利的生物力学条件。

{"title":"Finite element biomechanical comparison of bone-driven and occlusion-driven fibular flap positioning in lateral segmental mandibular reconstruction","authors":"Dmytro Filonenko , Yurii Chepurnyi , Mykola Kryshchuk , Bekir Osmanov , Andrii Kopchak","doi":"10.1016/j.jobcr.2026.101419","DOIUrl":"10.1016/j.jobcr.2026.101419","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate, using finite-element analysis (FEA), the stress–strain state of mandibular bone and the bone component of a microvascular free fibula flap (FFF) reconstructing a lateral segmental mandibular defect, comparing two flap positions relevant to implant-supported fixed prosthetic rehabilitation.</div></div><div><h3>Materials and methods</h3><div>CT-derived three-dimensional models of a reconstructed mandible were generated in two geometries: (A) flap aligned with the inferior mandibular border and (B) flap positioned at the level of the alveolar crest. Each geometry received two endosseous implants and a fixed prosthesis and was subjected to representative masticatory loading (vertical/occlusal and anterior/incisal). Four FEA models (2 positions × 2 load cases) were analyzed, with two predefined regions of interest per model (distal and medial), each examined at the upper and lower margins.</div></div><div><h3>Results</h3><div>Compared with Model A (inferior mandibular border), Model B (alveolar crest) produced lower peak bone stresses in most analyzed regions (≈10–40% reduction) under mastication-representative static molar and incisal loads. The only exception was the medial–inferior margin under incisal loading, where stress increased markedly (8.96 vs 2.22 MPa; ∼4-fold). Under occlusal loading, medial–inferior stresses were <1 MPa in both models. Across both positioning scenarios, peak crestal bone von Mises stresses clustered near the implant neck (crestal bone); analyses were restricted to bone.</div></div><div><h3>Conclusion</h3><div>A higher (alveolar-crest) position of the bone component of the FFF yields a more favorable stress–strain environment and load distribution, supporting more favorable biomechanical conditions for osseointegration and long-term function.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101419"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of zinc oxide and ellagic acid on macrophage infiltration, angiogenesis, and collagen deposition in excisional wounds healing 氧化锌和鞣花酸对切除伤口愈合中巨噬细胞浸润、血管生成和胶原沉积的协同作用

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-03-01 Epub Date: 2026-01-13 DOI: 10.1016/j.jobcr.2026.01.002

Intan Nirwana , Michelle Fidelia Alexandra , Tarissa Balqis Nuraida , Ahmad Thariq Bermani , Yasmin Shahaniz , Devi Rianti , Titien Hary Agustantina , Anita Yuliati , Khairul Annuar Shariff , Meircurius Dwi Condro Surboyo

Objectives

The combination of zinc oxide (ZnO) and ellagic acid (EA) in wound dressings offers synergistic benefits for wound healing due to their complementary antimicrobial, antioxidant, anti-inflammatory, and tissue regenerative properties. This study aimed to evaluate the effects of ZnO-EA dressing on macrophage infiltration, angiogenesis, and collagen density in excisional wounds on days 3 and 7 post-treatment.

Materials and methods

This in vivo posttest-only experimental study involved twenty Rattus norvegicus with excisional dorsal wounds, randomly assigned to two groups: a control group receiving ZnO dressing and a treatment group receiving ZnO-EA dressing. Each group was evaluated at day 3 and day 7, resulting in four observation subgroups. Tissue samples were collected to assess macrophage infiltration and angiogenesis using Hematoxylin and Eosin staining, and collagen density using Masson's Trichrome staining. Histological evaluation was performed under a light microscope at 400× magnification.

Results

Treatment with ZnO-EA resulted in significant increase in macrophages infiltration, the number of newly formed blood vessels, and collagen density in the treatment group compared to the ZnO group on both days 3 and 7 (p < 0.05).

Conclusion

The application of a ZnO wound dressing enriched with EA significantly enhances macrophage infiltration, angiogenesis, and collagen deposition during the wound healing process.

目的将氧化锌（ZnO）和鞣花酸（EA）结合在伤口敷料中，具有抗菌、抗氧化、抗炎和组织再生的协同作用。本研究旨在评价ZnO-EA敷料在治疗后第3天和第7天对切除创面巨噬细胞浸润、血管生成和胶原蛋白密度的影响。材料与方法本实验选取20只背部切除创面的褐家鼠，随机分为两组：对照组和治疗组分别采用ZnO- ea敷料。每组在第3天和第7天进行评估，形成4个观察亚组。收集组织样本，用苏木精和伊红染色评估巨噬细胞浸润和血管生成，用马松三色染色评估胶原蛋白密度。光镜下400倍放大进行组织学观察。结果与ZnO组相比，ZnO- ea治疗组在第3天和第7天巨噬细胞浸润、新生血管数量和胶原蛋白密度均显著增加（p < 0.05）。结论添加EA的氧化锌创面敷料可显著增强创面愈合过程中巨噬细胞的浸润、血管生成和胶原的沉积。

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引用次数: 0