Journal of oral biology and craniofacial research最新文献_第4页

Molecular mechanisms of human papillomavirus-induced tongue carcinogenesis: A systematic review 人乳头瘤病毒诱发舌癌的分子机制：系统综述

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-10 DOI: 10.1016/j.jobcr.2025.11.011

Chamathsara Hewa Kodikarage , Menaka Batuwanthudawa , Kalpani Senevirathna , Wasala Mudiyanselage Kalpani Madhushika Ratnayake , Sivasuntharam Induijaa , Yovanthi Anurangi Jayasinghe , Kehinde Kazeem Kanmodi , Bogahawatte Samarakoon Mudiyanselage Samadarani Siriwardena , Ruwan Duminda Jayasinghe

Background

Human papillomavirus (HPV) infection has emerged as a significant etiological factor in tongue cancer, particularly among individuals without conventional risk factors such as tobacco or alcohol use. Understanding the molecular mechanisms underlying HPV-induced tongue carcinogenesis is critical for advancing targeted interventions.

Objective

To systematically review existing literature on genetic and epigenetic alterations in HPV-associated tongue cancer, with emphasis on viral oncoprotein interactions, disrupted cellular signalling pathways, and immune evasion mechanisms.

Data sources

Electronic databases searched included PubMed, Scopus, Web of Science, Dentistry and Oral Sciences Source, and AMED.

Methods

A systematic search using relevant MeSH terms was conducted to identify peer-reviewed studies involving human participants. Duplicates were removed, and studies were screened using the Rayyan software based on predefined inclusion and exclusion criteria. Quality assessment was performed using the Mixed Methods Appraisal Tool (MMAT).

Results

The search yielded 3,140 articles, with 27 studies meeting the inclusion criteria after full-text screening and manual search. Quality assessment indicated that 96.3 % of studies (n = 26) had low risk of bias, while one study (3.7 %) had high risk. Key findings showed that HPV E6 and E7 oncoproteins interfere with tumor suppressor pathways (e.g., p53 and Rb), and activate PI3K/Akt, Wnt/β-catenin, and NF-κB signaling. Epigenetic alterations such as promoter hypermethylation, histone modification, and microRNA dysregulation were also implicated. HPV-positive tumours demonstrated immune evasion features.

Conclusion

Despite progress in understanding HPV-related tongue carcinogenesis, further research is needed to explore tissue tropism and identify novel therapeutic targets.

Prospero id

CRD42024593129.

人乳头瘤病毒（HPV）感染已成为舌癌的一个重要病因，特别是在没有吸烟或饮酒等传统危险因素的人群中。了解hpv诱发舌癌的分子机制对于推进有针对性的干预至关重要。目的系统回顾hpv相关舌癌遗传和表观遗传改变的现有文献，重点关注病毒癌蛋白相互作用、细胞信号通路中断和免疫逃避机制。检索的电子数据库包括PubMed、Scopus、Web of Science、Dentistry and Oral Sciences Source和AMED。方法使用相关MeSH术语进行系统检索，以识别涉及人类参与者的同行评议研究。删除重复，使用Rayyan软件根据预定义的纳入和排除标准筛选研究。采用混合方法评价工具（MMAT）进行质量评价。结果共检索到3140篇文献，经全文筛选和人工检索，符合纳入标准的文献有27篇。质量评价显示96.3%的研究（n = 26）具有低偏倚风险，1项研究（3.7%）具有高风险。关键发现显示，HPV E6和E7癌蛋白干扰肿瘤抑制通路（如p53和Rb），激活PI3K/Akt、Wnt/β-catenin和NF-κB信号。表观遗传改变，如启动子超甲基化、组蛋白修饰和microRNA失调也有牵连。hpv阳性肿瘤表现出免疫逃避特征。结论尽管对hpv相关舌癌发生的认识有所进展，但仍需进一步研究其组织趋向性和寻找新的治疗靶点。普洛斯彼罗idCRD42024593129。

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引用次数: 0

Predictive modeling of stem cell suppression by inflammatory cytokine networks: A synthetic transcriptomic approach for periodontal tissue engineering 炎症细胞因子网络对干细胞抑制的预测建模：牙周组织工程的合成转录组学方法

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-10 DOI: 10.1016/j.jobcr.2025.11.019

Deepavalli Arumuganainar , Pradeep Kumar Yadalam

Background

Chronic inflammation in periodontitis disrupts the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through persistent cytokine activity. IL-1β, TNF-α, and IL-6 are major mediators that inhibit bone-forming pathways. However, the complexity of cytokine–gene interactions remains poorly characterized. This study presents a synthetic transcriptomic modeling framework to predict and interpret inflammatory suppression of stem-cell osteogenesis.

Methods

Time-resolved synthetic gene expression profiles were generated to simulate osteogenic induction under homeostatic, inflammatory, and resolution phases. A curated gene regulatory network (GRN) was incorporated to map cytokine–osteogenesis interactions. Graph autoencoders (GAEs) captured latent topological structure from the expression matrix, while deep neural classifier differentiated inflammatory from control states. The GSE283726 periodontitis transcriptome dataset and iPSC-derived mesenchymal stem cells (iMSCs) were used for validation.

Results

Simulations showed that IL-1β and TNF-α strongly activated NF-κB signaling, suppressing osteogenic genes such as RUNX2 and ALPL. IL-6 exhibited context-dependent regulatory behavior. GAEs clearly separated inflammatory and regenerative modules, identifying IL-6 as a key intermediary. The classifier achieved an AUROC of 0.99 and > 95 % accuracy. Validation with real datasets confirmed overlap in differentially expressed genes and enriched pathways, including Wnt inhibition (DKK1) and inflammatory GO terms.

Conclusion

Biologically informed synthetic transcriptomics combined with graph autoencoding effectively models cytokine-mediated inhibition of PDLSCs. The framework identifies regulatory nodes supported by real data and offers potential for in silico drug testing. Future work will expand cytokine networks, incorporate diverse cell types, and explore transfer learning for regenerative periodontal applications.

背景牙周炎的慢性炎症通过持续的细胞因子活性破坏牙周韧带干细胞（PDLSCs）的成骨分化。IL-1β、TNF-α和IL-6是抑制骨形成途径的主要介质。然而，细胞因子-基因相互作用的复杂性仍然缺乏表征。本研究提出了一个合成转录组建模框架来预测和解释干细胞成骨的炎症抑制。方法合成时间分辨的合成基因表达谱，模拟体内平衡、炎症和消退阶段的成骨诱导。一个精心策划的基因调控网络（GRN）被纳入绘制细胞因子-成骨相互作用。图自编码器（GAEs）从表达矩阵中捕获潜在的拓扑结构，而深度神经分类器区分炎症和控制状态。使用GSE283726牙周炎转录组数据集和ipsc衍生的间充质干细胞（iMSCs）进行验证。结果IL-1β和TNF-α强烈激活NF-κB信号，抑制RUNX2、ALPL等成骨基因。IL-6表现出上下文依赖的调节行为。GAEs清楚地分离了炎症和再生模块，确定IL-6是关键中介。该分类器的AUROC为0.99，准确率为95%。真实数据集的验证证实了差异表达基因和富集通路的重叠，包括Wnt抑制（DKK1）和炎性氧化石墨烯术语。结论合成转录组学结合图形自编码有效地模拟了细胞因子介导的PDLSCs抑制。该框架确定了由真实数据支持的监管节点，并为计算机药物测试提供了潜力。未来的工作将扩大细胞因子网络，纳入不同的细胞类型，并探索迁移学习再生牙周的应用。

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引用次数: 0

Prevalence and distribution of shoveling and double shoveling non-metric traits in Mysuru population: A cross sectional study Mysuru人群铲铲和双铲非计量性状的流行和分布：一项横断面研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-10 DOI: 10.1016/j.jobcr.2025.12.006

G.R. Aiswarya , H.S. Sreeshyla , Swathi Kumareswar

Background

Non-metric dental traits play a pivotal role in anthropological and forensic investigations. Shovel-shaped incisors, in particular, serve as valuable indicators of population affinity and individual identification. This study aimed to assess the frequency and grading of the non-metric shoveling and double shoveling traits in the Mysuru population using the Arizona State University Dental Anthropology System (ASUDAS) scoring criteria.

Methodology

A cross-sectional descriptive study was conducted in Mysuru, among 382 individuals (190 males and 192 females) aged 18–40 years. Traits were assessed clinically using ASUDAS plaques. Data were recorded and analyzed using descriptive statistics and chi-square tests to determine prevalence and statistical significance across gender, arch, quadrant and tooth type.

Results

Shoveling was highly prevalent in the population (95.5 %), especially in upper central incisors (92.2 %), followed by upper lateral incisors (69.3 %). In contrast, lower anterior teeth exhibited minimal expression (<3 %). Double shoveling was rare, observed in only 1.0 % of individuals. No statistically significant difference in trait prevalence was found between genders or sides (p > 0.05). However, shoveling was significantly more common in the upper arch than the lower (p = 0.000). Grading analysis revealed Grade 1 shoveling as the most common form, particularly in upper central and lateral incisors. Double shoveling was mostly absent, with only trace levels (Grade 1) being noted.

Conclusion

The study highlights the prevalence of shoveling, especially in the maxillary anterior teeth among the Mysuru population. The results support the forensic and anthropological value of these traits in personal identification and population studies.

非计量牙齿特征在人类学和法医调查中发挥着关键作用。尤其是铲形门牙，可以作为种群亲和力和个体识别的有价值指标。本研究旨在使用亚利桑那州立大学牙科人类学系统（ASUDAS）评分标准评估Mysuru人群中非公制铲牙和双铲牙特征的频率和分级。方法采用横断面描述性研究方法，对382名年龄在18-40岁的个体（男性190名，女性192名）进行研究。使用ASUDAS斑块进行临床特征评估。采用描述性统计和卡方检验对数据进行记录和分析，以确定不同性别、牙弓、象限和牙型的患病率和统计学意义。结果人群中铲牙率最高（95.5%），以上中切牙最高（92.2%），其次为上侧切牙（69.3%）。相比之下，下前牙表达最少（< 3%）。双铲是罕见的，仅在1.0%的个体中观察到。各性别、各方面的性状患病率差异无统计学意义（p > 0.05）。然而，铲铲在上弓比下弓明显更常见（p = 0.000）。分级分析显示1级铲牙是最常见的形式，特别是在上中门牙和侧门牙。大多数情况下没有双铲，只有微量水平（1级）被记录。结论该研究突出了Mysuru人群中铲牙的患病率，特别是上颌前牙。研究结果支持了这些特征在个人鉴定和人口研究中的法医和人类学价值。

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引用次数: 0

Microsurgical management of gingival recession using pouch and tunnel technique with or without 20 % propolis irrigation: A randomized controlled trial 用眼袋和隧道技术治疗牙龈萎缩，有或没有20%蜂胶灌溉：一项随机对照试验

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-09 DOI: 10.1016/j.jobcr.2025.12.003

Akash Alok Bora, Sumit Malhotra, Joohi Chandra

Aim

To evaluate the adjunctive effect of 20 % propolis irrigation with platelet-rich fibrin (PRF) in the microsurgical Pouch and Tunnel technique for Miller's Class I and II gingival recession defects.

Materials and methods

Eighteen patients with Miller's Class I and II recession defects were randomly assigned to two groups: Group A (test, n = 16 sites) received 20 % propolis irrigation followed by the Pouch and Tunnel technique with PRF under a surgical microscope, while Group B (control, n = 13 sites) received the same procedure without propolis. Clinical parameters included Plaque Index (PI), Gingival Index (GI), Probing Depth (PD), Relative Clinical Attachment Level (RCAL), Recession Depth (RD), and Width of Keratinized Tissue (WKT) which were recorded at baseline and 3 months.

Results

Both groups showed significant intragroup improvement in GI, PI, RCAL, RD and WKT from baseline to 3 months. Intergroup comparison revealed significantly greater reduction in RD and gain in WKT in the propolis group (p < 0.05).

Conclusion

Within the study's limitations, adjunctive use of 20 % propolis irrigation with PRF enhanced root coverage outcomes and increased keratinized tissue width compared to PRF alone. These preliminary findings suggest that propolis may serve as a beneficial adjunct in minimally invasive periodontal plastic surgery.

目的评价富血小板纤维蛋白（PRF） 20%蜂胶灌洗在显微外科袋管技术治疗米勒氏1、2级牙龈退缩缺损中的辅助效果。材料与方法将18例Miller's I类和II类退行性缺损患者随机分为两组：A组（试验组，16个部位）在手术显微镜下采用20%蜂胶灌洗，然后用PRF进行袋管技术；B组（对照组，13个部位）采用相同的方法，但不使用蜂胶。临床参数包括牙菌斑指数（PI）、牙龈指数（GI）、探探深度（PD）、相对临床附着水平（RCAL）、退退深度（RD）和角化组织宽度（WKT），分别在基线和3个月时记录。结果两组患者GI、PI、RCAL、RD和WKT从基线到3个月均有组内显著改善。组间比较显示蜂胶组RD和WKT增重显著降低（p < 0.05）。结论：在研究的局限性内，与单独使用PRF相比，辅助使用20%蜂胶灌溉PRF可提高根部覆盖效果，并增加角化组织宽度。这些初步结果表明蜂胶可作为一种有益的辅助微创牙周整形手术。

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引用次数: 0

Detection of clinically relevant resistance genes in subgingival biofilms of chronic periodontitis: A cross-sectional molecular surveillance study 慢性牙周炎龈下生物膜中临床相关耐药基因的检测：一项横断面分子监测研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-09 DOI: 10.1016/j.jobcr.2025.12.002

Krishnasamy Nitya , K. Hema Shree , Aishwarya Arya , Sameep Shetty

Background

Antimicrobial resistance (AMR) has emerged as a major barrier to effective periodontal therapy, particularly when adjunctive antibiotics are prescribed empirically. The oral cavity—especially the subgingival biofilm—serves as a dynamic reservoir for antibiotic resistance genes (ARGs), yet data from the Indian population remain limited.

Aim

To detect and characterize the presence and co-occurrence of clinically relevant ARGs—tet(M), blaTEM, and erm(B)—within subgingival biofilms of Indian patients with chronic periodontitis through PCR-based molecular surveillance.

Methods

This cross-sectional pilot study (n = 20) was conducted among systemically healthy adults diagnosed with chronic periodontitis (2018 AAP-EFP criteria). The sample size was pragmatically defined to assess feasibility and prevalence trends. Subgingival plaque from the deepest pocket per patient (non-pooled) was collected before instrumentation by a single calibrated examiner. DNA was extracted and subjected to end-point PCR targeting tet(M), blaTEM, and erm(B), with gene-specific positive and no-template negative controls included in each run. Amplification reproducibility was verified through cross-laboratory concordance testing. Descriptive and Fisher's exact analyses were applied to explore gender-wise patterns.

Results

tet(M) was detected in 65 % of patients, blaTEM in 45 %, and erm(B) in 30 %. Dual-gene co-occurrence was observed in 35 %, while 20 % harbored all three genes. The most frequent association was tet(M) + blaTEM. Males exhibited a higher mean gene burden (1.8 ± 0.4) than females (1.4 ± 0.5), though differences were not statistically significant (p > 0.05).

Conclusion

This pilot demonstrates a substantial prevalence and co-occurrence of resistance determinants in periodontal biofilms, underscoring the oral cavity's role in local and systemic AMR propagation. While preliminary, these findings support incorporating molecular resistance surveillance into periodontal diagnostics and tailoring region-specific antibiotic stewardship strategies to preserve therapeutic efficacy.

抗生素耐药（AMR）已成为有效牙周治疗的主要障碍，特别是当辅助抗生素处方经验。口腔——尤其是龈下生物膜——是抗生素耐药基因（ARGs）的动态储存库，然而来自印度人群的数据仍然有限。目的通过基于聚合酶链反应（pcr）的分子监测，检测和表征印度慢性牙周炎患者龈下生物膜中临床相关的ARGs-tet (M)、blaTEM和erm(B) -的存在和共现情况。方法本横断面试点研究（n = 20）在诊断为慢性牙周炎（2018年AAP-EFP标准）的全身健康成人中进行。实际定义样本量以评估可行性和流行趋势。每位患者（非合并）最深牙袋的龈下菌斑在仪器前由一名校准的检查员收集。提取DNA，并对tet(M)、blaTEM和erm(B)进行终点PCR，每次运行均包括基因特异性阳性对照和无模板阴性对照。通过跨实验室一致性测试验证扩增重复性。描述性和Fisher的精确分析被应用于探索性别模式。结果65%的患者检出stet(M)， 45%的患者检出blaTEM， 30%的患者检出erm(B)。双基因共现者占35%，三种基因共现者占20%。最常见的关联是tet(M) + blaTEM。男性的平均基因负荷（1.8±0.4）高于女性（1.4±0.5），但差异无统计学意义（p > 0.05）。结论：该试验表明牙周生物膜中耐药决定因素的普遍存在和共同发生，强调了口腔在局部和全身AMR传播中的作用。虽然是初步的，但这些发现支持将分子耐药性监测纳入牙周诊断和定制特定区域的抗生素管理策略，以保持治疗效果。

{"title":"Detection of clinically relevant resistance genes in subgingival biofilms of chronic periodontitis: A cross-sectional molecular surveillance study","authors":"Krishnasamy Nitya , K. Hema Shree , Aishwarya Arya , Sameep Shetty","doi":"10.1016/j.jobcr.2025.12.002","DOIUrl":"10.1016/j.jobcr.2025.12.002","url":null,"abstract":"<div><h3>Background</h3><div>Antimicrobial resistance (AMR) has emerged as a major barrier to effective periodontal therapy, particularly when adjunctive antibiotics are prescribed empirically. The oral cavity—especially the subgingival biofilm—serves as a dynamic reservoir for antibiotic resistance genes (ARGs), yet data from the Indian population remain limited.</div></div><div><h3>Aim</h3><div>To detect and characterize the presence and co-occurrence of clinically relevant ARGs—<em>tet(M)</em>, <em>blaTEM</em>, and <em>erm(B)</em>—within subgingival biofilms of Indian patients with chronic periodontitis through PCR-based molecular surveillance.</div></div><div><h3>Methods</h3><div>This cross-sectional pilot study (n = 20) was conducted among systemically healthy adults diagnosed with chronic periodontitis (2018 AAP-EFP criteria). The sample size was pragmatically defined to assess feasibility and prevalence trends. Subgingival plaque from the deepest pocket per patient (non-pooled) was collected before instrumentation by a single calibrated examiner. DNA was extracted and subjected to end-point PCR targeting <em>tet(M)</em>, <em>blaTEM</em>, and <em>erm(B)</em>, with gene-specific positive and no-template negative controls included in each run. Amplification reproducibility was verified through cross-laboratory concordance testing. Descriptive and Fisher's exact analyses were applied to explore gender-wise patterns.</div></div><div><h3>Results</h3><div><em>tet(M)</em> was detected in 65 % of patients, <em>blaTEM</em> in 45 %, and <em>erm(B)</em> in 30 %. Dual-gene co-occurrence was observed in 35 %, while 20 % harbored all three genes. The most frequent association was <em>tet(M)</em> + <em>blaTEM</em>. Males exhibited a higher mean gene burden (1.8 ± 0.4) than females (1.4 ± 0.5), though differences were not statistically significant (<em>p</em> > 0.05).</div></div><div><h3>Conclusion</h3><div>This pilot demonstrates a substantial prevalence and co-occurrence of resistance determinants in periodontal biofilms, underscoring the oral cavity's role in local and systemic AMR propagation. While preliminary, these findings support incorporating molecular resistance surveillance into periodontal diagnostics and tailoring region-specific antibiotic stewardship strategies to preserve therapeutic efficacy.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 167-173"},"PeriodicalIF":0.0,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of infraorbital foramen location based on skeletal malocclusion, maxillary sinus pneumatization and infraorbital canal morphology using 3D CBCT scans 基于骨骼错颌合、上颌窦充气和眶下管形态的三维CBCT扫描评估眶下孔定位

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-06 DOI: 10.1016/j.jobcr.2025.12.001

Gülay Açar , Ahmet Safa Gökşan , Guldane Magat

Introduction

Understanding the infraorbital foramen (IOF) location is crucial in terms of regional anaesthesia as well as facial filler injection and radiofrequency neurotomy. We aimed to define a safe infraorbital foramen (IOF) location zone using three dimensional (3D) cone beam computed tomography (CBCT) scans and to evaluate the influence of skeletal malocclusion (SM), maxillary sinus pneumatization (MSP), and infraorbital canal (IOC) morphology on IOF position.

Materials and methods

3D models of 300 (170 female, 130 male, range 18–50 years) CBCT scans were used to identify the SM, MSP, and IOC types, and to measure the distances from IOF to infraorbital margin, pyriform aperture, and zygion to determine IOF position. Using combined distances the IOF location zone was formed and divided into nine zones.

Results

The IOF was predominantly located in the superocentral zones (Zones 1–6) in Class II patients, mostly associated with hypoplastic MS, Type I and IV IOCs. Conversely, in Class III patients mostly linked to hyperplastic MS, Type II and III IOCs, the likelihood of IOF was mainly observed in the inferocentral zones (Zones 4–9). Also, we observed a significant increase in the prevalence of Class I, hypoplastic MS, Type I and IV IOCs with age.

Conclusion

Our results suggest that prediction of a safe IOF zone is possible based on SM, MSP, and IOC types in relation to gender and age within the studied population using 3D CBCT technology. This is essential for improved treatment planning and avoidance of iatrogenic injury during midface procedures.

了解眶下孔（IOF）的位置对于区域麻醉、面部填充物注射和射频神经切开术至关重要。我们的目的是使用三维（3D）锥束计算机断层扫描（CBCT）确定一个安全的眶下孔（IOF）定位区域，并评估骨骼错颌合（SM）、上颌窦气化（MSP）和眶下管（IOC）形态对IOF位置的影响。材料与方法使用300例（女性170例，男性130例，年龄18-50岁）的三维CBCT扫描模型来识别SM、MSP和IOC类型，并测量IOF到眶下缘、梨状孔和颧部的距离来确定IOF的位置。利用综合距离形成了IOF定位区，并将其划分为9个区域。结果II类患者IOF主要位于超中心区（1-6区），多与发育不良的MS、I型和IV型ioc相关。相反，在主要与增生性MS、II型和III型ioc相关的III类患者中，IOF的可能性主要在中央间区（4-9区）观察到。此外，我们还观察到，随着年龄的增长，I类、发育不良的MS、I型和IV型ioc的患病率显著增加。结论：我们的研究结果表明，使用3D CBCT技术可以根据研究人群中与性别和年龄相关的SM、MSP和IOC类型来预测安全的IOF区域。这对于改善治疗计划和避免中面部手术中医源性损伤是至关重要的。

{"title":"Assessment of infraorbital foramen location based on skeletal malocclusion, maxillary sinus pneumatization and infraorbital canal morphology using 3D CBCT scans","authors":"Gülay Açar , Ahmet Safa Gökşan , Guldane Magat","doi":"10.1016/j.jobcr.2025.12.001","DOIUrl":"10.1016/j.jobcr.2025.12.001","url":null,"abstract":"<div><h3>Introduction</h3><div>Understanding the infraorbital foramen (IOF) location is crucial in terms of regional anaesthesia as well as facial filler injection and radiofrequency neurotomy. We aimed to define a safe infraorbital foramen (IOF) location zone using three dimensional (3D) cone beam computed tomography (CBCT) scans and to evaluate the influence of skeletal malocclusion (SM), maxillary sinus pneumatization (MSP), and infraorbital canal (IOC) morphology on IOF position.</div></div><div><h3>Materials and methods</h3><div>3D models of 300 (170 female, 130 male, range 18–50 years) CBCT scans were used to identify the SM, MSP, and IOC types, and to measure the distances from IOF to infraorbital margin, pyriform aperture, and zygion to determine IOF position. Using combined distances the IOF location zone was formed and divided into nine zones.</div></div><div><h3>Results</h3><div>The IOF was predominantly located in the superocentral zones (Zones 1–6) in Class II patients, mostly associated with hypoplastic MS, Type I and IV IOCs. Conversely, in Class III patients mostly linked to hyperplastic MS, Type II and III IOCs, the likelihood of IOF was mainly observed in the inferocentral zones (Zones 4–9). Also, we observed a significant increase in the prevalence of Class I, hypoplastic MS, Type I and IV IOCs with age.</div></div><div><h3>Conclusion</h3><div>Our results suggest that prediction of a safe IOF zone is possible based on SM, MSP, and IOC types in relation to gender and age within the studied population using 3D CBCT technology. This is essential for improved treatment planning and avoidance of iatrogenic injury during midface procedures.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 160-166"},"PeriodicalIF":0.0,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145690656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical effectiveness and cost-effectiveness of two sodium fluoride varnish systems in preventing dental caries in children: a randomized clinical trial 两种氟化钠清漆系统预防儿童龋齿的临床效果和成本效益：一项随机临床试验

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-02 DOI: 10.1016/j.jobcr.2025.11.018

Mahesh R. Khairnar , P.G. Naveen Kumar , Harloveen Sabharwal , Sachin Kumar Jadhav , Sheetal Badnaware , Neha Shukla , Ananta Kusumakar , Zainab Akram , Savitha Priyadarsini S , Ridhi Ghodela

Background

Dental caries frequently affects first permanent molars soon after eruption due to their anatomical susceptibility. Fluoride varnish is a widely accepted preventive measure; however, different formulations vary in fluoride retention and cost. Evidence comparing both clinical outcomes and economic value of available varnishes in children remains limited.

Aim

To compare the clinical effectiveness and cost-effectiveness of resin-based and alcohol-based 5 % sodium fluoride varnishes in preventing dental caries in permanent first molars of high-risk schoolchildren.

Design

A double-blind, parallel-arm randomized controlled trial was conducted among 84 children (330 M) aged 6–8 years from a private school in Varanasi, India. Participants received either resin-based varnish (163 teeth) or alcohol-based varnish (167 teeth) at six-month intervals. Caries incidence was assessed at baseline, 6, 12, 18, and 24 months using ICDAS-II. A financial cost-benefit perspective was adopted, considering direct material and application costs borne by the provider and estimated restorative treatment savings for patients. Appropriate statistical tests were used for analysis (α = 0.05).

Results

Caries incidence remained low in both groups after 24 months (resin-based: 7.4 %; alcohol-based: 7.8 %; p = 0.961). No significant differences were found in early or advanced lesions at any interval (p > 0.05). ITT and Per-protocol analyses revealed negligible effect sizes (Cliff's Delta ≤0.05). Cost-effectiveness analysis demonstrated that the alcohol-based varnish produced ₹62.22 savings per ₹1 spent, compared to ₹8.47 in resin-based group. The cost to save ₹100 in future restorative expenses was ₹1.61 (alcohol-based) and ₹11.80 (resin-based).

Conclusion

Both varnishes were equally effective, but alcohol-based varnish was substantially more cost-effective, making it a suitable choice for large-scale preventive programs in resource-constrained settings.

由于第一恒磨牙在解剖学上的易感性，龋齿经常在出牙后不久发生。氟化物清漆是一种广泛接受的预防措施；然而，不同的配方在氟化物保留和成本方面有所不同。比较儿童可用清漆的临床结果和经济价值的证据仍然有限。目的比较树脂基和醇基5%氟化钠清漆预防高危学童恒牙第一磨牙龋病的临床效果和成本效益。设计对印度瓦拉纳西一所私立学校的84名6-8岁儿童（330名）进行了双盲、平行组随机对照试验。参与者每六个月接受一次树脂基清漆（163颗牙齿）或酒精基清漆（167颗牙齿）。使用ICDAS-II评估基线、6、12、18和24个月的龋发病率。采用财务成本-效益的观点，考虑由提供者承担的直接材料和应用成本以及估计患者的恢复性治疗节省。采用适当的统计学检验进行分析（α = 0.05）。结果24个月后，两组患者的惊恐发生率均较低（树脂基：7.4%；酒精基：7.8%;p = 0.961）。早期和晚期病变在任何间隔均无显著差异（p > 0.05）。ITT和Per-protocol分析显示效应大小可以忽略不计（Cliff’s Delta≤0.05）。成本效益分析表明，醇基清漆每花费1卢比节省62.22卢比，而树脂基组为8.47卢比。在未来修复费用中节省100卢比的成本为1.61卢比（酒精基）和11.80卢比（树脂基）。结论两种清漆的效果相同，但醇基清漆的成本效益更高，是资源紧张地区大规模预防项目的合适选择。

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引用次数: 0

In-vitro evaluation of shear bond strength and bond-failure of isoamyl cyanoacrylate-resin adhesive ratios for bonding 3D-Printed denture bases and teeth 体外评价3d打印义齿基托与牙齿的剪切粘接强度和粘接破坏

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-01 DOI: 10.1016/j.jobcr.2025.11.017

Divyansh Sinha, Ram Kiran, Suresh Venugopalan

Statement of problem

Shear bond strength between 3D-printed denture bases and teeth is inferior compared to conventional and milled dentures.

Purpose

The success of 3D-printed complete dentures depends on reliable bonding between the denture base and teeth. This study evaluated shear bond strength and modes of bond failure of various isoamyl cyanoacrylate (ICA) and 3D printing resin formulations in different ratios.

Materials and methods

Adhesive formulations of standardized ICA-to-resin ratios were prepared. Fifty samples were randomized into five groups: 0 % (control), 25 %, 50 %, 75 %, and 100 % ICA. Specimens were fabricated per ISO/TS 19736 standards using 3D-printed denture base and tooth resins. After bonding and polymerization, samples underwent shear bond strength testing. Failure modes were examined under stereomicroscope and classified as adhesive, cohesive, or mixed. Data were analyzed using one-way ANOVA with Tukey's post hoc test.

Results

The 50 % ICA group showed the highest mean shear bond strength (3.738 ± 0.063 MPa), significantly greater than all others (P = 0.000). The control group exhibited the lowest strength (1.602 ± 0.140 MPa). Bond failure analysis showed predominantly adhesive failures in the control, whereas the 50 % ICA group had the greatest proportion of cohesive and mixed failures, indicating improved interfacial bonding and internal strength.

Conclusions

A 50:50 ratio of isoamyl cyanoacrylate and light-cure 3D printing resin provides optimal bond strength and favorable failure patterns for bonding 3D-printed denture bases and teeth suggesting that a hybrid adhesive approach offers a promising solution to enhance the durability of 3D-printed dentures.

问题陈述3d打印义齿基托和牙齿之间的剪切结合强度不如传统和磨假牙。目的3d打印全口义齿的成功与否取决于义齿基托与牙齿之间的可靠结合。本研究评估了不同比例的氰基丙烯酸酯异戊酯（ICA）和3D打印树脂配方的剪切粘结强度和粘结破坏模式。材料与方法制备了标准ica -树脂配比的胶粘剂配方。50例样本随机分为5组：0%（对照）、25%、50%、75%和100% ICA。使用3d打印义齿基托和牙树脂，按照ISO/TS 19736标准制作标本。粘结聚合后，试样进行剪切粘结强度测试。在体视显微镜下检查了破坏模式，并将其分为粘接、内聚或混合。数据分析采用单因素方差分析和Tukey事后检验。结果50% ICA组的平均剪切强度最高（3.738±0.063 MPa），显著高于其他各组（P = 0.000）。对照组强度最低（1.602±0.140 MPa）。粘合失效分析显示，在对照组中主要是粘合失效，而50%的ICA组有最大比例的内聚和混合失效，表明界面粘合和内部强度得到改善。结论50:50比例的氰基丙烯酸酯异戊酯和光固化3D打印树脂可以为3D打印义齿基托与牙齿的粘接提供最佳的粘接强度和良好的失效模式，表明混合粘接方法是提高3D打印义齿耐久性的一种有前景的解决方案。

{"title":"In-vitro evaluation of shear bond strength and bond-failure of isoamyl cyanoacrylate-resin adhesive ratios for bonding 3D-Printed denture bases and teeth","authors":"Divyansh Sinha, Ram Kiran, Suresh Venugopalan","doi":"10.1016/j.jobcr.2025.11.017","DOIUrl":"10.1016/j.jobcr.2025.11.017","url":null,"abstract":"<div><h3>Statement of problem</h3><div>Shear bond strength between 3D-printed denture bases and teeth is inferior compared to conventional and milled dentures.</div></div><div><h3>Purpose</h3><div>The success of 3D-printed complete dentures depends on reliable bonding between the denture base and teeth. This study evaluated shear bond strength and modes of bond failure of various isoamyl cyanoacrylate (ICA) and 3D printing resin formulations in different ratios.</div></div><div><h3>Materials and methods</h3><div>Adhesive formulations of standardized ICA-to-resin ratios were prepared. Fifty samples were randomized into five groups: 0 % (control), 25 %, 50 %, 75 %, and 100 % ICA. Specimens were fabricated per ISO/TS 19736 standards using 3D-printed denture base and tooth resins. After bonding and polymerization, samples underwent shear bond strength testing. Failure modes were examined under stereomicroscope and classified as adhesive, cohesive, or mixed. Data were analyzed using one-way ANOVA with Tukey's post hoc test.</div></div><div><h3>Results</h3><div>The 50 % ICA group showed the highest mean shear bond strength (3.738 ± 0.063 MPa), significantly greater than all others (P = 0.000). The control group exhibited the lowest strength (1.602 ± 0.140 MPa). Bond failure analysis showed predominantly adhesive failures in the control, whereas the 50 % ICA group had the greatest proportion of cohesive and mixed failures, indicating improved interfacial bonding and internal strength.</div></div><div><h3>Conclusions</h3><div>A 50:50 ratio of isoamyl cyanoacrylate and light-cure 3D printing resin provides optimal bond strength and favorable failure patterns for bonding 3D-printed denture bases and teeth suggesting that a hybrid adhesive approach offers a promising solution to enhance the durability of 3D-printed dentures.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 146-152"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145690536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered taste perception in Type 2 Diabetes Mellitus: A case-control study on taste thresholds and glycemic control 2型糖尿病味觉感知改变：味觉阈值和血糖控制的病例对照研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-30 DOI: 10.1016/j.jobcr.2025.11.015

Julika De , Hina Handa , Gaurav Arya , Sanskriti Agrawal , Anirban Das , Swagata Saha

Background and objectives

Diabetes Mellitus (DM) is associated with altered taste perception, often leading to cravings for carbohydrate-rich foods. Although taste dysfunction has been linked to diabetic neuropathy, this association is debatable. Few studies have evaluated taste thresholds in individuals with diabetes, necessitating further investigations. This study aimed to assess gustatory function in patients with Type 2 Diabetes Mellitus (T2DM).

Materials and methods

A total of 138 participants were included in the study: 46 with controlled T2DM, 46 with uncontrolled T2DM, and 46 healthy controls. Taste perception was assessed using whole-mouth threshold and spatial tests. Data were analysed using the Kruskal-Wallis test, followed by Dunn-Bonferroni post hoc tests for pairwise comparisons. The significance level (p) was set at 0.05 for all statistical analyses.

Results

HbA1c levels were highest in the non-controlled diabetes group (11.22%), followed by the controlled DM group (7.43%) and the control group (5.08%) (p < 0.001). Whole-mouth threshold and spatial taste tests showed significant taste impairment in patients with diabetes, worsening with poor glycaemic control (p < 0.001), particularly for sweet, sour, and bitter tastes. Salt perception was unaffected (p = 0.56).

Conclusion

Individuals with diabetes exhibit taste dysfunction, particularly for sweet, sour, and bitter tastes. Blunted sweet perception may lead to increased sugar intake, worsening hyperglycaemia. These findings highlight the need for dietary counselling to mitigate the impact of taste impairment on glycaemic control.

背景与目的糖尿病（DM）与味觉改变有关，通常导致对富含碳水化合物的食物的渴望。虽然味觉功能障碍与糖尿病神经病变有关，但这种联系是有争议的。很少有研究评估糖尿病患者的味觉阈值，这需要进一步的研究。本研究旨在评估2型糖尿病（T2DM）患者的味觉功能。材料和方法本研究共纳入138名参与者：46名T2DM患者，46名未控制T2DM患者，46名健康对照。味觉知觉通过全口阈值和空间测试进行评估。采用Kruskal-Wallis检验分析数据，随后采用Dunn-Bonferroni事后检验进行两两比较。所有统计分析的显著性水平(p)设为0.05。结果非控制糖尿病组患者shba1c水平最高（11.22%），其次为控制糖尿病组（7.43%）和对照组（5.08%）（p < 0.001）。全口阈值和空间味觉测试显示，糖尿病患者有明显的味觉障碍，血糖控制不良会加重（p < 0.001），尤其是甜、酸和苦的味觉。对盐的感知不受影响（p = 0.56）。结论糖尿病患者存在味觉功能障碍，尤其是甜、酸、苦味觉功能障碍。甜味感知迟钝可能导致糖摄入量增加，加剧高血糖。这些发现强调了饮食咨询的必要性，以减轻味觉障碍对血糖控制的影响。

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引用次数: 0

Comparative evaluation of breathing convenience and clinical acceptance of a new patented mouth breathing device for rubber dam: A randomized clinical study 一种新型橡胶坝口呼吸装置的呼吸便利性和临床接受度的比较评价：一项随机临床研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-28 DOI: 10.1016/j.jobcr.2025.11.013

Geetanjali Jain, Ajay Singh Rao, Shreya Volety, Vidula Bhandary, Rutvi Karande

Introduction

The rubber dam isolation technique is widely used in endodontics to improve visibility and access, yet it often causes patient discomfort, especially for those with difficulty breathing through their nose. To address this, a new patented mouth breathing device (MBD) was developed to enhance patient comfort by facilitating easier mouth breathing during rubber dam isolation. This study aims to evaluate the breathing convenience and clinical acceptance of the MBD.

Methods

In a randomized clinical trial, 36 patients undergoing endodontic procedures with rubber dam isolation were recruited. Participants were randomly assigned to either the experimental group (MBD) or the control group (no device). A custom-developed questionnaire was used to assess patient comfort, ease of breathing, and overall satisfaction with the procedure. Clinicians also provided feedback on the device's impact on treatment efficiency, patient cooperation, and clinical outcomes. Data were analyzed using statistical tests to compare the results between the two groups.

Results

The study evaluated 36 participants, evenly divided into two groups with a significant age difference (p = 0.04). Group A (Test) showed improved outcomes, including reduced blood pressure (SBP: 14.12 mmHg, DBP: 16.76 mmHg) and increased oxygen saturation (SpO2: +5.71 %, p < 0.001). In contrast, Group B (Control) showed worsened outcomes with increased blood pressure and decreased SpO2 (−6.12 %, p < 0.001).

Conclusion

The patented mouth breathing device offers significant benefits in terms of breathing convenience and patient comfort during rubber dam isolation. The device appears to be a promising adjunct to improve clinical outcomes by reducing patient anxiety and discomfort.

橡胶坝隔离技术广泛应用于牙髓学，以提高可视性和可及性，但它经常引起患者不适，特别是对那些通过鼻子呼吸困难的患者。为了解决这个问题，一种新的口腔呼吸装置（MBD）被开发出来，通过在橡胶坝隔离期间促进更容易的口腔呼吸来提高患者的舒适度。本研究旨在评估MBD的呼吸便利性和临床接受度。方法采用随机临床试验方法，选取36例采用橡胶坝隔离术的根管治疗患者。参与者被随机分配到实验组（MBD）或对照组（无设备）。使用定制开发的问卷来评估患者的舒适度、呼吸的轻松程度和对手术的总体满意度。临床医生还就该设备对治疗效率、患者合作和临床结果的影响提供了反馈。采用统计学检验对数据进行分析，比较两组结果。结果本研究共评估了36名参与者，平均分为两组，年龄差异显著（p = 0.04）。A组（试验）表现出改善的结果，包括血压降低（收缩压：14.12 mmHg，舒张压：16.76 mmHg）和血氧饱和度升高（SpO2: + 5.71%, p < 0.001）。相比之下，B组（对照组）的预后恶化，血压升高，SpO2降低（- 6.12%,p < 0.001）。结论本发明专利口腔呼吸装置在橡胶坝隔离术中呼吸方便，患者舒适度显著提高。该设备似乎是一种很有希望的辅助手段，可以通过减少患者的焦虑和不适来改善临床结果。

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引用次数: 0