Journal of oral biology and craniofacial research最新文献_第4页

Microsurgical management of gingival recession using pouch and tunnel technique with or without 20 % propolis irrigation: A randomized controlled trial 用眼袋和隧道技术治疗牙龈萎缩，有或没有20%蜂胶灌溉：一项随机对照试验

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-09 DOI: 10.1016/j.jobcr.2025.12.003

Akash Alok Bora, Sumit Malhotra, Joohi Chandra

Aim

To evaluate the adjunctive effect of 20 % propolis irrigation with platelet-rich fibrin (PRF) in the microsurgical Pouch and Tunnel technique for Miller's Class I and II gingival recession defects.

Materials and methods

Eighteen patients with Miller's Class I and II recession defects were randomly assigned to two groups: Group A (test, n = 16 sites) received 20 % propolis irrigation followed by the Pouch and Tunnel technique with PRF under a surgical microscope, while Group B (control, n = 13 sites) received the same procedure without propolis. Clinical parameters included Plaque Index (PI), Gingival Index (GI), Probing Depth (PD), Relative Clinical Attachment Level (RCAL), Recession Depth (RD), and Width of Keratinized Tissue (WKT) which were recorded at baseline and 3 months.

Results

Both groups showed significant intragroup improvement in GI, PI, RCAL, RD and WKT from baseline to 3 months. Intergroup comparison revealed significantly greater reduction in RD and gain in WKT in the propolis group (p < 0.05).

Conclusion

Within the study's limitations, adjunctive use of 20 % propolis irrigation with PRF enhanced root coverage outcomes and increased keratinized tissue width compared to PRF alone. These preliminary findings suggest that propolis may serve as a beneficial adjunct in minimally invasive periodontal plastic surgery.

目的评价富血小板纤维蛋白（PRF） 20%蜂胶灌洗在显微外科袋管技术治疗米勒氏1、2级牙龈退缩缺损中的辅助效果。材料与方法将18例Miller's I类和II类退行性缺损患者随机分为两组：A组（试验组，16个部位）在手术显微镜下采用20%蜂胶灌洗，然后用PRF进行袋管技术；B组（对照组，13个部位）采用相同的方法，但不使用蜂胶。临床参数包括牙菌斑指数（PI）、牙龈指数（GI）、探探深度（PD）、相对临床附着水平（RCAL）、退退深度（RD）和角化组织宽度（WKT），分别在基线和3个月时记录。结果两组患者GI、PI、RCAL、RD和WKT从基线到3个月均有组内显著改善。组间比较显示蜂胶组RD和WKT增重显著降低（p < 0.05）。结论：在研究的局限性内，与单独使用PRF相比，辅助使用20%蜂胶灌溉PRF可提高根部覆盖效果，并增加角化组织宽度。这些初步结果表明蜂胶可作为一种有益的辅助微创牙周整形手术。

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引用次数: 0

Detection of clinically relevant resistance genes in subgingival biofilms of chronic periodontitis: A cross-sectional molecular surveillance study 慢性牙周炎龈下生物膜中临床相关耐药基因的检测：一项横断面分子监测研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-09 DOI: 10.1016/j.jobcr.2025.12.002

Krishnasamy Nitya , K. Hema Shree , Aishwarya Arya , Sameep Shetty

Background

Antimicrobial resistance (AMR) has emerged as a major barrier to effective periodontal therapy, particularly when adjunctive antibiotics are prescribed empirically. The oral cavity—especially the subgingival biofilm—serves as a dynamic reservoir for antibiotic resistance genes (ARGs), yet data from the Indian population remain limited.

Aim

To detect and characterize the presence and co-occurrence of clinically relevant ARGs—tet(M), blaTEM, and erm(B)—within subgingival biofilms of Indian patients with chronic periodontitis through PCR-based molecular surveillance.

Methods

This cross-sectional pilot study (n = 20) was conducted among systemically healthy adults diagnosed with chronic periodontitis (2018 AAP-EFP criteria). The sample size was pragmatically defined to assess feasibility and prevalence trends. Subgingival plaque from the deepest pocket per patient (non-pooled) was collected before instrumentation by a single calibrated examiner. DNA was extracted and subjected to end-point PCR targeting tet(M), blaTEM, and erm(B), with gene-specific positive and no-template negative controls included in each run. Amplification reproducibility was verified through cross-laboratory concordance testing. Descriptive and Fisher's exact analyses were applied to explore gender-wise patterns.

Results

tet(M) was detected in 65 % of patients, blaTEM in 45 %, and erm(B) in 30 %. Dual-gene co-occurrence was observed in 35 %, while 20 % harbored all three genes. The most frequent association was tet(M) + blaTEM. Males exhibited a higher mean gene burden (1.8 ± 0.4) than females (1.4 ± 0.5), though differences were not statistically significant (p > 0.05).

Conclusion

This pilot demonstrates a substantial prevalence and co-occurrence of resistance determinants in periodontal biofilms, underscoring the oral cavity's role in local and systemic AMR propagation. While preliminary, these findings support incorporating molecular resistance surveillance into periodontal diagnostics and tailoring region-specific antibiotic stewardship strategies to preserve therapeutic efficacy.

抗生素耐药（AMR）已成为有效牙周治疗的主要障碍，特别是当辅助抗生素处方经验。口腔——尤其是龈下生物膜——是抗生素耐药基因（ARGs）的动态储存库，然而来自印度人群的数据仍然有限。目的通过基于聚合酶链反应（pcr）的分子监测，检测和表征印度慢性牙周炎患者龈下生物膜中临床相关的ARGs-tet (M)、blaTEM和erm(B) -的存在和共现情况。方法本横断面试点研究（n = 20）在诊断为慢性牙周炎（2018年AAP-EFP标准）的全身健康成人中进行。实际定义样本量以评估可行性和流行趋势。每位患者（非合并）最深牙袋的龈下菌斑在仪器前由一名校准的检查员收集。提取DNA，并对tet(M)、blaTEM和erm(B)进行终点PCR，每次运行均包括基因特异性阳性对照和无模板阴性对照。通过跨实验室一致性测试验证扩增重复性。描述性和Fisher的精确分析被应用于探索性别模式。结果65%的患者检出stet(M)， 45%的患者检出blaTEM， 30%的患者检出erm(B)。双基因共现者占35%，三种基因共现者占20%。最常见的关联是tet(M) + blaTEM。男性的平均基因负荷（1.8±0.4）高于女性（1.4±0.5），但差异无统计学意义（p > 0.05）。结论：该试验表明牙周生物膜中耐药决定因素的普遍存在和共同发生，强调了口腔在局部和全身AMR传播中的作用。虽然是初步的，但这些发现支持将分子耐药性监测纳入牙周诊断和定制特定区域的抗生素管理策略，以保持治疗效果。

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引用次数: 0

Assessment of infraorbital foramen location based on skeletal malocclusion, maxillary sinus pneumatization and infraorbital canal morphology using 3D CBCT scans 基于骨骼错颌合、上颌窦充气和眶下管形态的三维CBCT扫描评估眶下孔定位

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-06 DOI: 10.1016/j.jobcr.2025.12.001

Gülay Açar , Ahmet Safa Gökşan , Guldane Magat

Introduction

Understanding the infraorbital foramen (IOF) location is crucial in terms of regional anaesthesia as well as facial filler injection and radiofrequency neurotomy. We aimed to define a safe infraorbital foramen (IOF) location zone using three dimensional (3D) cone beam computed tomography (CBCT) scans and to evaluate the influence of skeletal malocclusion (SM), maxillary sinus pneumatization (MSP), and infraorbital canal (IOC) morphology on IOF position.

Materials and methods

3D models of 300 (170 female, 130 male, range 18–50 years) CBCT scans were used to identify the SM, MSP, and IOC types, and to measure the distances from IOF to infraorbital margin, pyriform aperture, and zygion to determine IOF position. Using combined distances the IOF location zone was formed and divided into nine zones.

Results

The IOF was predominantly located in the superocentral zones (Zones 1–6) in Class II patients, mostly associated with hypoplastic MS, Type I and IV IOCs. Conversely, in Class III patients mostly linked to hyperplastic MS, Type II and III IOCs, the likelihood of IOF was mainly observed in the inferocentral zones (Zones 4–9). Also, we observed a significant increase in the prevalence of Class I, hypoplastic MS, Type I and IV IOCs with age.

Conclusion

Our results suggest that prediction of a safe IOF zone is possible based on SM, MSP, and IOC types in relation to gender and age within the studied population using 3D CBCT technology. This is essential for improved treatment planning and avoidance of iatrogenic injury during midface procedures.

了解眶下孔（IOF）的位置对于区域麻醉、面部填充物注射和射频神经切开术至关重要。我们的目的是使用三维（3D）锥束计算机断层扫描（CBCT）确定一个安全的眶下孔（IOF）定位区域，并评估骨骼错颌合（SM）、上颌窦气化（MSP）和眶下管（IOC）形态对IOF位置的影响。材料与方法使用300例（女性170例，男性130例，年龄18-50岁）的三维CBCT扫描模型来识别SM、MSP和IOC类型，并测量IOF到眶下缘、梨状孔和颧部的距离来确定IOF的位置。利用综合距离形成了IOF定位区，并将其划分为9个区域。结果II类患者IOF主要位于超中心区（1-6区），多与发育不良的MS、I型和IV型ioc相关。相反，在主要与增生性MS、II型和III型ioc相关的III类患者中，IOF的可能性主要在中央间区（4-9区）观察到。此外，我们还观察到，随着年龄的增长，I类、发育不良的MS、I型和IV型ioc的患病率显著增加。结论：我们的研究结果表明，使用3D CBCT技术可以根据研究人群中与性别和年龄相关的SM、MSP和IOC类型来预测安全的IOF区域。这对于改善治疗计划和避免中面部手术中医源性损伤是至关重要的。

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引用次数: 0

Clinical effectiveness and cost-effectiveness of two sodium fluoride varnish systems in preventing dental caries in children: a randomized clinical trial 两种氟化钠清漆系统预防儿童龋齿的临床效果和成本效益：一项随机临床试验

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-02 DOI: 10.1016/j.jobcr.2025.11.018

Mahesh R. Khairnar , P.G. Naveen Kumar , Harloveen Sabharwal , Sachin Kumar Jadhav , Sheetal Badnaware , Neha Shukla , Ananta Kusumakar , Zainab Akram , Savitha Priyadarsini S , Ridhi Ghodela

Background

Dental caries frequently affects first permanent molars soon after eruption due to their anatomical susceptibility. Fluoride varnish is a widely accepted preventive measure; however, different formulations vary in fluoride retention and cost. Evidence comparing both clinical outcomes and economic value of available varnishes in children remains limited.

Aim

To compare the clinical effectiveness and cost-effectiveness of resin-based and alcohol-based 5 % sodium fluoride varnishes in preventing dental caries in permanent first molars of high-risk schoolchildren.

Design

A double-blind, parallel-arm randomized controlled trial was conducted among 84 children (330 M) aged 6–8 years from a private school in Varanasi, India. Participants received either resin-based varnish (163 teeth) or alcohol-based varnish (167 teeth) at six-month intervals. Caries incidence was assessed at baseline, 6, 12, 18, and 24 months using ICDAS-II. A financial cost-benefit perspective was adopted, considering direct material and application costs borne by the provider and estimated restorative treatment savings for patients. Appropriate statistical tests were used for analysis (α = 0.05).

Results

Caries incidence remained low in both groups after 24 months (resin-based: 7.4 %; alcohol-based: 7.8 %; p = 0.961). No significant differences were found in early or advanced lesions at any interval (p > 0.05). ITT and Per-protocol analyses revealed negligible effect sizes (Cliff's Delta ≤0.05). Cost-effectiveness analysis demonstrated that the alcohol-based varnish produced ₹62.22 savings per ₹1 spent, compared to ₹8.47 in resin-based group. The cost to save ₹100 in future restorative expenses was ₹1.61 (alcohol-based) and ₹11.80 (resin-based).

Conclusion

Both varnishes were equally effective, but alcohol-based varnish was substantially more cost-effective, making it a suitable choice for large-scale preventive programs in resource-constrained settings.

由于第一恒磨牙在解剖学上的易感性，龋齿经常在出牙后不久发生。氟化物清漆是一种广泛接受的预防措施；然而，不同的配方在氟化物保留和成本方面有所不同。比较儿童可用清漆的临床结果和经济价值的证据仍然有限。目的比较树脂基和醇基5%氟化钠清漆预防高危学童恒牙第一磨牙龋病的临床效果和成本效益。设计对印度瓦拉纳西一所私立学校的84名6-8岁儿童（330名）进行了双盲、平行组随机对照试验。参与者每六个月接受一次树脂基清漆（163颗牙齿）或酒精基清漆（167颗牙齿）。使用ICDAS-II评估基线、6、12、18和24个月的龋发病率。采用财务成本-效益的观点，考虑由提供者承担的直接材料和应用成本以及估计患者的恢复性治疗节省。采用适当的统计学检验进行分析（α = 0.05）。结果24个月后，两组患者的惊恐发生率均较低（树脂基：7.4%；酒精基：7.8%;p = 0.961）。早期和晚期病变在任何间隔均无显著差异（p > 0.05）。ITT和Per-protocol分析显示效应大小可以忽略不计（Cliff’s Delta≤0.05）。成本效益分析表明，醇基清漆每花费1卢比节省62.22卢比，而树脂基组为8.47卢比。在未来修复费用中节省100卢比的成本为1.61卢比（酒精基）和11.80卢比（树脂基）。结论两种清漆的效果相同，但醇基清漆的成本效益更高，是资源紧张地区大规模预防项目的合适选择。

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引用次数: 0

In-vitro evaluation of shear bond strength and bond-failure of isoamyl cyanoacrylate-resin adhesive ratios for bonding 3D-Printed denture bases and teeth 体外评价3d打印义齿基托与牙齿的剪切粘接强度和粘接破坏

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-12-01 DOI: 10.1016/j.jobcr.2025.11.017

Divyansh Sinha, Ram Kiran, Suresh Venugopalan

Statement of problem

Shear bond strength between 3D-printed denture bases and teeth is inferior compared to conventional and milled dentures.

Purpose

The success of 3D-printed complete dentures depends on reliable bonding between the denture base and teeth. This study evaluated shear bond strength and modes of bond failure of various isoamyl cyanoacrylate (ICA) and 3D printing resin formulations in different ratios.

Materials and methods

Adhesive formulations of standardized ICA-to-resin ratios were prepared. Fifty samples were randomized into five groups: 0 % (control), 25 %, 50 %, 75 %, and 100 % ICA. Specimens were fabricated per ISO/TS 19736 standards using 3D-printed denture base and tooth resins. After bonding and polymerization, samples underwent shear bond strength testing. Failure modes were examined under stereomicroscope and classified as adhesive, cohesive, or mixed. Data were analyzed using one-way ANOVA with Tukey's post hoc test.

Results

The 50 % ICA group showed the highest mean shear bond strength (3.738 ± 0.063 MPa), significantly greater than all others (P = 0.000). The control group exhibited the lowest strength (1.602 ± 0.140 MPa). Bond failure analysis showed predominantly adhesive failures in the control, whereas the 50 % ICA group had the greatest proportion of cohesive and mixed failures, indicating improved interfacial bonding and internal strength.

Conclusions

A 50:50 ratio of isoamyl cyanoacrylate and light-cure 3D printing resin provides optimal bond strength and favorable failure patterns for bonding 3D-printed denture bases and teeth suggesting that a hybrid adhesive approach offers a promising solution to enhance the durability of 3D-printed dentures.

问题陈述3d打印义齿基托和牙齿之间的剪切结合强度不如传统和磨假牙。目的3d打印全口义齿的成功与否取决于义齿基托与牙齿之间的可靠结合。本研究评估了不同比例的氰基丙烯酸酯异戊酯（ICA）和3D打印树脂配方的剪切粘结强度和粘结破坏模式。材料与方法制备了标准ica -树脂配比的胶粘剂配方。50例样本随机分为5组：0%（对照）、25%、50%、75%和100% ICA。使用3d打印义齿基托和牙树脂，按照ISO/TS 19736标准制作标本。粘结聚合后，试样进行剪切粘结强度测试。在体视显微镜下检查了破坏模式，并将其分为粘接、内聚或混合。数据分析采用单因素方差分析和Tukey事后检验。结果50% ICA组的平均剪切强度最高（3.738±0.063 MPa），显著高于其他各组（P = 0.000）。对照组强度最低（1.602±0.140 MPa）。粘合失效分析显示，在对照组中主要是粘合失效，而50%的ICA组有最大比例的内聚和混合失效，表明界面粘合和内部强度得到改善。结论50:50比例的氰基丙烯酸酯异戊酯和光固化3D打印树脂可以为3D打印义齿基托与牙齿的粘接提供最佳的粘接强度和良好的失效模式，表明混合粘接方法是提高3D打印义齿耐久性的一种有前景的解决方案。

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引用次数: 0

Altered taste perception in Type 2 Diabetes Mellitus: A case-control study on taste thresholds and glycemic control 2型糖尿病味觉感知改变：味觉阈值和血糖控制的病例对照研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-30 DOI: 10.1016/j.jobcr.2025.11.015

Julika De , Hina Handa , Gaurav Arya , Sanskriti Agrawal , Anirban Das , Swagata Saha

Background and objectives

Diabetes Mellitus (DM) is associated with altered taste perception, often leading to cravings for carbohydrate-rich foods. Although taste dysfunction has been linked to diabetic neuropathy, this association is debatable. Few studies have evaluated taste thresholds in individuals with diabetes, necessitating further investigations. This study aimed to assess gustatory function in patients with Type 2 Diabetes Mellitus (T2DM).

Materials and methods

A total of 138 participants were included in the study: 46 with controlled T2DM, 46 with uncontrolled T2DM, and 46 healthy controls. Taste perception was assessed using whole-mouth threshold and spatial tests. Data were analysed using the Kruskal-Wallis test, followed by Dunn-Bonferroni post hoc tests for pairwise comparisons. The significance level (p) was set at 0.05 for all statistical analyses.

Results

HbA1c levels were highest in the non-controlled diabetes group (11.22%), followed by the controlled DM group (7.43%) and the control group (5.08%) (p < 0.001). Whole-mouth threshold and spatial taste tests showed significant taste impairment in patients with diabetes, worsening with poor glycaemic control (p < 0.001), particularly for sweet, sour, and bitter tastes. Salt perception was unaffected (p = 0.56).

Conclusion

Individuals with diabetes exhibit taste dysfunction, particularly for sweet, sour, and bitter tastes. Blunted sweet perception may lead to increased sugar intake, worsening hyperglycaemia. These findings highlight the need for dietary counselling to mitigate the impact of taste impairment on glycaemic control.

背景与目的糖尿病（DM）与味觉改变有关，通常导致对富含碳水化合物的食物的渴望。虽然味觉功能障碍与糖尿病神经病变有关，但这种联系是有争议的。很少有研究评估糖尿病患者的味觉阈值，这需要进一步的研究。本研究旨在评估2型糖尿病（T2DM）患者的味觉功能。材料和方法本研究共纳入138名参与者：46名T2DM患者，46名未控制T2DM患者，46名健康对照。味觉知觉通过全口阈值和空间测试进行评估。采用Kruskal-Wallis检验分析数据，随后采用Dunn-Bonferroni事后检验进行两两比较。所有统计分析的显著性水平(p)设为0.05。结果非控制糖尿病组患者shba1c水平最高（11.22%），其次为控制糖尿病组（7.43%）和对照组（5.08%）（p < 0.001）。全口阈值和空间味觉测试显示，糖尿病患者有明显的味觉障碍，血糖控制不良会加重（p < 0.001），尤其是甜、酸和苦的味觉。对盐的感知不受影响（p = 0.56）。结论糖尿病患者存在味觉功能障碍，尤其是甜、酸、苦味觉功能障碍。甜味感知迟钝可能导致糖摄入量增加，加剧高血糖。这些发现强调了饮食咨询的必要性，以减轻味觉障碍对血糖控制的影响。

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引用次数: 0

Comparative evaluation of breathing convenience and clinical acceptance of a new patented mouth breathing device for rubber dam: A randomized clinical study 一种新型橡胶坝口呼吸装置的呼吸便利性和临床接受度的比较评价：一项随机临床研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-28 DOI: 10.1016/j.jobcr.2025.11.013

Geetanjali Jain, Ajay Singh Rao, Shreya Volety, Vidula Bhandary, Rutvi Karande

Introduction

The rubber dam isolation technique is widely used in endodontics to improve visibility and access, yet it often causes patient discomfort, especially for those with difficulty breathing through their nose. To address this, a new patented mouth breathing device (MBD) was developed to enhance patient comfort by facilitating easier mouth breathing during rubber dam isolation. This study aims to evaluate the breathing convenience and clinical acceptance of the MBD.

Methods

In a randomized clinical trial, 36 patients undergoing endodontic procedures with rubber dam isolation were recruited. Participants were randomly assigned to either the experimental group (MBD) or the control group (no device). A custom-developed questionnaire was used to assess patient comfort, ease of breathing, and overall satisfaction with the procedure. Clinicians also provided feedback on the device's impact on treatment efficiency, patient cooperation, and clinical outcomes. Data were analyzed using statistical tests to compare the results between the two groups.

Results

The study evaluated 36 participants, evenly divided into two groups with a significant age difference (p = 0.04). Group A (Test) showed improved outcomes, including reduced blood pressure (SBP: 14.12 mmHg, DBP: 16.76 mmHg) and increased oxygen saturation (SpO2: +5.71 %, p < 0.001). In contrast, Group B (Control) showed worsened outcomes with increased blood pressure and decreased SpO2 (−6.12 %, p < 0.001).

Conclusion

The patented mouth breathing device offers significant benefits in terms of breathing convenience and patient comfort during rubber dam isolation. The device appears to be a promising adjunct to improve clinical outcomes by reducing patient anxiety and discomfort.

橡胶坝隔离技术广泛应用于牙髓学，以提高可视性和可及性，但它经常引起患者不适，特别是对那些通过鼻子呼吸困难的患者。为了解决这个问题，一种新的口腔呼吸装置（MBD）被开发出来，通过在橡胶坝隔离期间促进更容易的口腔呼吸来提高患者的舒适度。本研究旨在评估MBD的呼吸便利性和临床接受度。方法采用随机临床试验方法，选取36例采用橡胶坝隔离术的根管治疗患者。参与者被随机分配到实验组（MBD）或对照组（无设备）。使用定制开发的问卷来评估患者的舒适度、呼吸的轻松程度和对手术的总体满意度。临床医生还就该设备对治疗效率、患者合作和临床结果的影响提供了反馈。采用统计学检验对数据进行分析，比较两组结果。结果本研究共评估了36名参与者，平均分为两组，年龄差异显著（p = 0.04）。A组（试验）表现出改善的结果，包括血压降低（收缩压：14.12 mmHg，舒张压：16.76 mmHg）和血氧饱和度升高（SpO2: + 5.71%, p < 0.001）。相比之下，B组（对照组）的预后恶化，血压升高，SpO2降低（- 6.12%,p < 0.001）。结论本发明专利口腔呼吸装置在橡胶坝隔离术中呼吸方便，患者舒适度显著提高。该设备似乎是一种很有希望的辅助手段，可以通过减少患者的焦虑和不适来改善临床结果。

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引用次数: 0

A novel histopathological subclassification of oral submucous fibrosis based on epithelial keratinization: A proof-of-concept pilot observation 基于上皮角化的口腔黏膜下纤维化的一种新的组织病理学亚分类：概念验证的先导观察

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-27 DOI: 10.1016/j.jobcr.2025.11.012

Gargi S. Sarode, Sachin C. Sarode

Oral submucous fibrosis (OSMF) is a chronic, progressive oral potentially malignant disorder strongly associated with areca nut consumption. Despite extensive research, a reliable and clinically applicable biomarker for predicting malignant transformation remains unavailable. The present pilot investigation introduces a novel concept by classifying OSMF into two histopathological variants—hyperkeratinized and non-hyperkeratinized—based on epithelial keratinization patterns, a distinction not previously described in the literature. Archival biopsy samples of 51 histologically confirmed OSMF cases from the buccal mucosa were analyzed for keratinization type and epithelial dysplasia. Additionally, 37 cases of oral squamous cell carcinoma arising in the background of OSMF (OSCC-OSMF) were examined for keratinization at surgical margins. Among OSMF cases, 19.6 % exhibited hyperkeratinization, all showing high-risk epithelial dysplasia, whereas 90.2 % of normally keratinized cases demonstrated low-risk dysplasia. In OSCC-OSMF specimens, 60 % of surgical margins revealed hyperkeratinization. These findings suggest that epithelial hyperkeratinization may represent an early histological alteration associated with malignant transformation rather than a protective epithelial response. Although limited by small sample size, this study provides proof-of-concept for a novel histopathological subclassification of OSMF, warranting validation through larger, multicentre longitudinal and molecular correlation studies.

口腔黏膜下纤维化（OSMF）是一种慢性进行性口腔潜在恶性疾病，与槟榔的食用密切相关。尽管广泛的研究，一个可靠的和临床适用的生物标志物预测恶性转化仍然没有。目前的试点研究引入了一个新概念，将OSMF分为两种组织病理学变异-基于上皮角化模式的角化过度和非角化过度，这是以前文献中未描述的区别。对51例经组织学证实的口腔粘膜OSMF病例的档案活检样本进行角化类型和上皮异常增生分析。此外，我们还对37例OSMF背景下发生的口腔鳞状细胞癌（OSCC-OSMF）进行了手术边缘角化检查。在OSMF病例中，19.6%表现为角化过度，均表现为高风险上皮异常增生，而正常角化的病例中，90.2%表现为低风险异常增生。在OSCC-OSMF标本中，60%的手术边缘显示角化过度。这些发现表明上皮过度角化可能代表与恶性转化相关的早期组织学改变，而不是保护性上皮反应。尽管受样本量的限制，本研究为OSMF的一种新的组织病理学亚分类提供了概念证明，需要通过更大的、多中心的纵向和分子相关研究来验证。

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引用次数: 0

Biocompatibility and odontogenic potential of an indigenous apitherapeutic pulp capping agent: A comparative in vitro analysis using hDPSCs 一种本土蜂疗牙髓封盖剂的生物相容性和牙源性潜力：利用hdpsc进行体外比较分析

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-25 DOI: 10.1016/j.jobcr.2025.11.008

Ketaki Turbatmath , S. Delphine Priscilla Antony , Raghunandhakumar Subramanian

Introduction

This study aimed to evaluate the cytocompatibility and odonto-inductive potential of an indigenously developed apitherapeutic pulp capping agent Api-Therapeutic Pulp Guard (ATPG) by assessing cell viability and dentinogenic gene expression using human dental pulp stem cells (hDPSCs)

Materials and methods

hDPSCs were exposed to eluates of ATPG and compared with calcium hydroxide (RC-Cal) and control. MTT assay was used to assess cell viability, LIVE/DEAD staining was performed using calcein-AM and ethidium homodimer, and quantitative real-time PCR (qPCR) evaluated DSPP gene expression. This comprehensive approach provided complementary insights into cell metabolic activity, membrane integrity, and odontogenic potential of the test materials.

Results

ATPG exhibited high cell viability (96.52 %), significantly greater than calcium hydroxide (84.25 %) (p < 0.01). LIVE/DEAD assay confirmed better membrane integrity with a predominance of green (live) stained cells. qPCR showed a 3.6-fold upregulation of DSPP gene expression in ATPG-treated cells, suggesting strong odontogenic differentiation.

Conclusion

The ATPG formulation demonstrated superior biocompatibility and odontogenic potential compared to conventional calcium hydroxide, underscoring its promise for translation in regenerative endodontic therapy.

本研究旨在通过评估人牙髓干细胞（hDPSCs）的细胞活力和牙本质形成基因表达，来评估国产牙髓盖盖剂Api-Therapeutic pulp Guard （ATPG）的细胞相容性和牙髓诱导潜能。材料和方法：将dpscs暴露于ATPG洗脱液中，并与氢氧化钙（RC-Cal）和对照进行比较。MTT法检测细胞活力，钙黄素am和乙啶同型二聚体进行LIVE/DEAD染色，定量实时荧光定量PCR （qPCR）检测DSPP基因表达。这种全面的方法为测试材料的细胞代谢活性、膜完整性和牙源性潜力提供了补充的见解。结果satpg具有较高的细胞存活率（96.52%），显著高于氢氧化钙（84.25%）（p < 0.01）。LIVE/DEAD实验证实了更好的膜完整性，绿色（活）染色细胞占主导地位。qPCR结果显示，atpg处理后的细胞DSPP基因表达上调3.6倍，显示出强烈的成牙分化。结论与传统氢氧化钙相比，ATPG制剂具有更好的生物相容性和牙源性潜力，在再生牙髓治疗中具有广阔的应用前景。

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引用次数: 0

SFXN1 expression analysis in oral squamous cell carcinoma and its association with the PI3K-AKT-mTOR pathway and immune cell infiltration SFXN1在口腔鳞状细胞癌中的表达分析及其与PI3K-AKT-mTOR通路和免疫细胞浸润的关系

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-25 DOI: 10.1016/j.jobcr.2025.11.010

Prabhu Manickam Natarajan , Manoj Kumar Karuppan Perumal , Sudhir Rama Varma , Sam Thomas Kuriadom , Ruba Odeh , Remya Rajan Renuka

Background

Oral squamous cell carcinoma (OSCC) is a predominant malignancy characterized by aggressive progression and poor prognosis. This study investigated the role of SFXN1 (Sideroflexin 1), a mitochondrial serine transporter, in OSCC using integrated bioinformatic and experimental approaches.

Objective

To analyze the expression, clinical relevance, and functional associations of SFXN1 in OSCC through comprehensive bioinformatic and in vitro investigations.

Methods

Transcriptomic data from TCGA pan-cancer cohorts were analyzed to evaluate SFXN1 expression patterns. The expression levels in OSCC were validated using KB OC cell lines, with clinical correlations assessed for tumor grade, nodal status, and patient survival. Immune infiltration associations and protein‒protein interaction networks were constructed, followed by pathway enrichment analyses. Experimental validation was performed via in vitro assays.

Results

The results revealed that SFXN1 was significantly overexpressed in head and neck squamous cell carcinoma and markedly upregulated in KB cells compared with controls. SFXN1 expression was associated with tumor grade and nodal metastasis, although no significant stage-specific differences were observed. Survival analysis revealed no statistically significant association with overall survival. Immune infiltration analysis indicated modest but significant correlations between SFXN1 and immune cell populations, particularly CD4⁺ T cells. Protein network analysis identified hub genes, including AKT1, BCL2, MTOR, and CASP3. Pathway enrichment implicated SFXN1 is involved in the PI3K-AKT-mTOR and p53 signaling pathways.

Conclusion

These findings highlight the involvement of SFXN1 in cancer-related pathways and its potential role in OSCC, suggesting potential therapeutic targeting opportunities that need further investigation.

背景：口腔鳞状细胞癌（OSCC）是一种主要的恶性肿瘤，其特点是进展积极，预后差。本研究采用生物信息学和实验相结合的方法研究了线粒体丝氨酸转运蛋白SFXN1 （Sideroflexin 1）在OSCC中的作用。目的通过综合生物信息学和体外研究，分析SFXN1在OSCC中的表达、临床意义和功能关联。方法分析来自TCGA泛癌症队列的转录组学数据，评估SFXN1表达模式。使用KB OC细胞系验证OSCC中的表达水平，并评估肿瘤分级、淋巴结状态和患者生存率的临床相关性。构建免疫浸润关联和蛋白-蛋白相互作用网络，然后进行途径富集分析。通过体外分析进行实验验证。结果与对照组相比，SFXN1在头颈部鳞状细胞癌中显著过表达，在KB细胞中显著上调。SFXN1表达与肿瘤分级和淋巴结转移有关，但未观察到明显的分期特异性差异。生存分析显示与总生存无统计学意义的关联。免疫浸润分析显示SFXN1与免疫细胞群，特别是CD4+ T细胞之间存在适度但显著的相关性。蛋白网络分析鉴定出枢纽基因，包括AKT1、BCL2、MTOR和CASP3。通路富集暗示SFXN1参与PI3K-AKT-mTOR和p53信号通路。结论这些发现突出了SFXN1参与癌症相关通路及其在OSCC中的潜在作用，提示潜在的治疗靶向机会需要进一步研究。

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引用次数: 0