The intricate process of chewing and swallowing is compromised following mandibular resection. An evaluation of chewing and swallowing is crucial to comprehend the quality of life following surgery in cases when bone repair was not performed. The purpose of this study is to evaluate chewing and swallowing in mandibulectomy patients without bony reconstruction.
Material and method
This study involved ten patients, including four with H defects, three with L defects, and three with LC defects, after a one-year postop period. Using Robbin's penetration aspiration scale, video fluoroscopy was used to evaluate swallowing. Using a functional oral intake scale, chewing was assessed. Fischer's exact test was used for statistical analysis.
Results
Robbin's penetration aspiration scale score of 1 was noted in all 10 patients. According to FOIS, 50 % of patients with H defect scored 4, and 66.7 % of those with L defect scored 6. The results were not significant when the scores were correlated with the type of defect.
Conclusion
Mandibular surgical defects which were not reconstructed with bone were shown to affect the quality of life in our study. Chewing efficiency was poorest in patients with H defect. Swallowing efficiency was not affected adversely in this group of patients.
{"title":"Assessment of chewing and swallowing in post mandibular resection patients with no bony reconstruction – A cross sectional study","authors":"Irfana Sithara Cholayil, Reena Rachel John, Narendran Achuthan","doi":"10.1016/j.jobcr.2024.12.014","DOIUrl":"10.1016/j.jobcr.2024.12.014","url":null,"abstract":"<div><h3>Introduction</h3><div>The intricate process of chewing and swallowing is compromised following mandibular resection. An evaluation of chewing and swallowing is crucial to comprehend the quality of life following surgery in cases when bone repair was not performed. The purpose of this study is to evaluate chewing and swallowing in mandibulectomy patients without bony reconstruction.</div></div><div><h3>Material and method</h3><div>This study involved ten patients, including four with H defects, three with L defects, and three with LC defects, after a one-year postop period. Using Robbin's penetration aspiration scale, video fluoroscopy was used to evaluate swallowing. Using a functional oral intake scale, chewing was assessed. Fischer's exact test was used for statistical analysis.</div></div><div><h3>Results</h3><div>Robbin's penetration aspiration scale score of 1 was noted in all 10 patients. According to FOIS, 50 % of patients with H defect scored 4, and 66.7 % of those with L defect scored 6. The results were not significant when the scores were correlated with the type of defect.</div></div><div><h3>Conclusion</h3><div>Mandibular surgical defects which were not reconstructed with bone were shown to affect the quality of life in our study. Chewing efficiency was poorest in patients with H defect. Swallowing efficiency was not affected adversely in this group of patients.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 159-162"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143177745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To prospectively evaluate the long-term effects of orthognathic surgery on masticatory function in individuals with repaired complete cleft lip and palate and to compare the results with a control group.
In the PRE-ORTHOG phase, the BF for the CLP group ( RM + LM = 285 ± 141) was significantly lower compared to the CON group ( RM + LM = 524 ± 202). In the POST-ORTHOG phase, the CLP group ( RM + LM = 373 ± 129) showed significant improvements in BF in relation to the PRE-ORTHOG phase, with values similar to those of the CON group. Masticatory efficiency improved significantly in the POST-ORTHOG phase (0.222 ± 0.071) compared to PRE-ORTHOG (0.470 ± 0.126) in the CLP group, while PRE-ORTHOG values were worse than those of the CON group (0.148 ± 0.050). Furthermore, 45 % of CLP participants reported P/R MC before surgery, while none reported this after surgery, a statistically significant improvement. The POST-ORTHOG MC results for CLP participants were comparable to the CON group, with 100 % reporting G/O MC after surgery.
Conclusions
Overall, the group with CLP demonstrated impaired masticatory function in the preoperative phase compared to the control group across all variables analyzed. Orthognathic surgery improves masticatory function in patients with CLP, with postoperative parameters comparable to those of the control group.
Pub Date : 2025-01-01DOI: 10.1016/j.jobcr.2024.12.006
Sapna Rani, Pankaj Dhawan, Vidushi Saxena
Aim
The aim of the present study was to comparatively evaluate the retention of complete dentures and oral health-related quality of life (OHRQoL) of patients with conventional and bioelectric impressions or transcutaneous electric nerve stimulation (TENS).
Materials and methods
A total of thirty (n = 30) completely edentulous patients were randomly distributed into two groups: Group-C (n = 15) (Conventional) and Group-T (n = 15) (bioelectric). In Group C, border molding was performed using the manual manipulation of borders, and the final impression was made using zinc-oxide eugenol. In Group T, border molding and final impression were performed with the TENS application. Dentures were fabricated, and retention was evaluated with a digital gauge. OHRQoL was assessed at insertion and after three months of follow-up using the Oral Health Impact Profile Edentulous in Hindi (OHIP-Edent-H) questionnaire. A two-way ANOVA test was used for the significance level (P < 0.05). Student t-test was used for intergroup comparison, and paired t-test was used for intragroup comparison of OHRQoL.
Results
There was no significant difference in the retention of maxillary and mandibular dentures fabricated with both techniques (P = 0.283, P = 0.682). Although, OHRQoL increased significantly in both groups at follow-up (P = 0.001). However, at follow-up, the Functional Limitation (FL) domain was significantly better in the conventional group.
Conclusions
Although, there was no significant difference in retention and OHRQoL between both groups. However, more clinical trials with large samples and longer follow-ups are required to reach a conclusive result.
{"title":"A randomized clinical trial comparing retention of complete dentures and oral health quality of life of patients with conventional and bioelectric impressions","authors":"Sapna Rani, Pankaj Dhawan, Vidushi Saxena","doi":"10.1016/j.jobcr.2024.12.006","DOIUrl":"10.1016/j.jobcr.2024.12.006","url":null,"abstract":"<div><h3>Aim</h3><div>The aim of the present study was to comparatively evaluate the retention of complete dentures and oral health-related quality of life (OHRQoL) of patients with conventional and bioelectric impressions or transcutaneous electric nerve stimulation (TENS).</div></div><div><h3>Materials and methods</h3><div>A total of thirty (n = 30) completely edentulous patients were randomly distributed into two groups: Group-C (n = 15) (Conventional) and Group-T (n = 15) (bioelectric). In Group C, border molding was performed using the manual manipulation of borders, and the final impression was made using zinc-oxide eugenol. In Group T, border molding and final impression were performed with the TENS application. Dentures were fabricated, and retention was evaluated with a digital gauge. OHRQoL was assessed at insertion and after three months of follow-up using the Oral Health Impact Profile Edentulous in Hindi (OHIP-Edent-H) questionnaire. A two-way ANOVA test was used for the significance level (<em>P</em> < 0.05). Student t-test was used for intergroup comparison, and paired <em>t</em>-test was used for intragroup comparison of OHRQoL.</div></div><div><h3>Results</h3><div>There was no significant difference in the retention of maxillary and mandibular dentures fabricated with both techniques (<em>P</em> = 0.283, <em>P</em> = 0.682). Although, OHRQoL increased significantly in both groups at follow-up (<em>P</em> = 0.001). However, at follow-up, the Functional Limitation (FL) domain was significantly better in the conventional group.</div></div><div><h3>Conclusions</h3><div>Although, there was no significant difference in retention and OHRQoL between both groups. However, more clinical trials with large samples and longer follow-ups are required to reach a conclusive result.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 103-107"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.jobcr.2024.12.011
R. Vijayalakshmi , N. Ambalavanan , S. Rajeshkumar , Jaideep Mahendra , Uma Sudhakar , Devi Parameswari
Background
This study compared the drug release kinetics of Terminalia arjuna mediated selenium nanoparticles (SeNPs) gel and zinc oxide nanoparticles (ZnONPs) gel for their potential in local drug delivery for chronic periodontitis.
Material and method
The drug release was evaluated in-vitro by conducting tests on different formulations, including 1 %, 2 %, 3 %, 4 %, and 5 % Terminalia arjuna mediated SeNPs gel and ZnONPs gel. Each sample, approximately 0.1 mg, was mixed with 10 mL of phosphate buffer saline (PBS) at various pH levels and maintained at 37 °C. The suspension was then placed in an incubated shaker at 120 rpm for 1 h. Five-milliliter samples were withdrawn from the dissolution medium at 30-min intervals and replaced with fresh PBS buffer to maintain a constant volume. The released drug amount was measured using a UV spectrophotometer (Systronics, India) at 290 nm.
Result
The investigation revealed that SeNPs gel exhibited higher drug release percentages compared to ZnONPs gel across various concentrations and time points. The sustained release profiles of both formulations suggest effective control over drug release, maintaining therapeutic drug levels over an extended period. The near-complete release of the drug at 500 min highlights the potential for prolonged therapeutic efficacy, reducing the need for frequent dosing and enhancing patient compliance.
Conclusion
Terminalia arjuna mediated SeNPs gel shows promise for more rapid and sustained drug delivery in the management of chronic periodontitis through local drug delivery systems.
{"title":"Comparative drug release kinetics of Terminalia arjuna mediated SeNPs NanoGel and ZnONPs NanoGel – An in-vitro study","authors":"R. Vijayalakshmi , N. Ambalavanan , S. Rajeshkumar , Jaideep Mahendra , Uma Sudhakar , Devi Parameswari","doi":"10.1016/j.jobcr.2024.12.011","DOIUrl":"10.1016/j.jobcr.2024.12.011","url":null,"abstract":"<div><h3>Background</h3><div>This study compared the drug release kinetics of <em>Terminalia arjuna</em> mediated selenium nanoparticles (SeNPs) gel and zinc oxide nanoparticles (ZnONPs) gel for their potential in local drug delivery for chronic periodontitis.</div></div><div><h3>Material and method</h3><div>The drug release was evaluated in-vitro by conducting tests on different formulations, including 1 %, 2 %, 3 %, 4 %, and 5 % <em>Terminalia arjuna</em> mediated SeNPs gel and ZnONPs gel. Each sample, approximately 0.1 mg, was mixed with 10 mL of phosphate buffer saline (PBS) at various pH levels and maintained at 37 °C. The suspension was then placed in an incubated shaker at 120 rpm for 1 h. Five-milliliter samples were withdrawn from the dissolution medium at 30-min intervals and replaced with fresh PBS buffer to maintain a constant volume. The released drug amount was measured using a UV spectrophotometer (Systronics, India) at 290 nm.</div></div><div><h3>Result</h3><div>The investigation revealed that SeNPs gel exhibited higher drug release percentages compared to ZnONPs gel across various concentrations and time points. The sustained release profiles of both formulations suggest effective control over drug release, maintaining therapeutic drug levels over an extended period. The near-complete release of the drug at 500 min highlights the potential for prolonged therapeutic efficacy, reducing the need for frequent dosing and enhancing patient compliance.</div></div><div><h3>Conclusion</h3><div><em>Terminalia arjuna</em> mediated SeNPs gel shows promise for more rapid and sustained drug delivery in the management of chronic periodontitis through local drug delivery systems.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 199-204"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143177724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/S2212-4268(25)00037-5
{"title":"Acknowledgement to Reviewers","authors":"","doi":"10.1016/S2212-4268(25)00037-5","DOIUrl":"10.1016/S2212-4268(25)00037-5","url":null,"abstract":"","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 212-213"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.jobcr.2024.11.007
Dakshinya M , Anitha P , A.S. Smiline Girija , Paramasivam A , Vijayashree Priyadharsini J
Background
Periodontitis is considered to be one of the major risk factors associated with cancers of the oral cavity. Periodontogenic pathogens such as Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans are the important pathogens associated with periodontitis. Chronic exposure to bacterial components induces changes in the nearby cells. Hence, the present study has been designed to identify the molecular mechanisms that could be associated with the two disease conditions viz., periodontitis and head and neck cancer.
Objective
The present study investigated the differential gene expression profile in human gingival keratinocytes treated with P. gingivalis (Pg), a bacterium associated with periodontal disease, and its possible association with the development of Head and Neck Squamous Cell Carcinoma (HNSCC).
Methods
The study followed a computational design. Multiple tools and databases, such as GEOmnibus, STRING, Metascape, PANTHER, and UALCAN, cBioportal, were used to derive an association between gene expression during infection with P. gingivalis, and the resulting gene expression profiles were analyzed in the HNSCC dataset.
Results
The study revealed 29 genes from a pool of transcripts acquired after comparing the Pg-HIGK and Sham-HIGK. Among them, 3 genes i.e., FST, VRK3, and SGK1, were found to be overexpressed and significantly influenced patient survival. The upregulation of FST was found to correlate with poor prognosis in HNSCC patients.
Conclusion
The study provided insights into the possible association of FST, VRK3 and SGK1 in the development of HNSCC. Further investigations are warranted to confirm the functional role of these genes in establishing the cancer phenotype in patients with chronic infection with Pg.
{"title":"Differential gene expression profile in Porphyromonas gingivalis treated human gingival keratinocytes and their role in the development of HNSCC","authors":"Dakshinya M , Anitha P , A.S. Smiline Girija , Paramasivam A , Vijayashree Priyadharsini J","doi":"10.1016/j.jobcr.2024.11.007","DOIUrl":"10.1016/j.jobcr.2024.11.007","url":null,"abstract":"<div><h3>Background</h3><div>Periodontitis is considered to be one of the major risk factors associated with cancers of the oral cavity. Periodontogenic pathogens such as <em>Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Fusobacterium nucleatum</em> and <em>Aggregatibacter actinomycetemcomitans</em> are the important pathogens associated with periodontitis. Chronic exposure to bacterial components induces changes in the nearby cells. Hence, the present study has been designed to identify the molecular mechanisms that could be associated with the two disease conditions <em>viz.,</em> periodontitis and head and neck cancer.</div></div><div><h3>Objective</h3><div>The present study investigated the differential gene expression profile in human gingival keratinocytes treated with <em>P. gingivalis (Pg),</em> a bacterium associated with periodontal disease, and its possible association with the development of Head and Neck Squamous Cell Carcinoma (HNSCC).</div></div><div><h3>Methods</h3><div>The study followed a computational design. Multiple tools and databases, such as GEOmnibus, STRING, Metascape, PANTHER, and UALCAN, cBioportal, were used to derive an association between gene expression during infection with <em>P. gingivalis,</em> and the resulting gene expression profiles were analyzed in the HNSCC dataset.</div></div><div><h3>Results</h3><div>The study revealed 29 genes from a pool of transcripts acquired after comparing the Pg-HIGK and Sham-HIGK. Among them, 3 genes i.e., <em>FST, VRK3,</em> and <em>SGK1,</em> were found to be overexpressed and significantly influenced patient survival. The upregulation of <em>FST</em> was found to correlate with poor prognosis in HNSCC patients.</div></div><div><h3>Conclusion</h3><div>The study provided insights into the possible association of <em>FST, VRK3</em> and <em>SGK1</em> in the development of HNSCC. Further investigations are warranted to confirm the functional role of these genes in establishing the cancer phenotype in patients with chronic infection with <em>Pg</em>.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 48-56"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.jobcr.2024.12.002
Ratri Maya Sitalaksmi , Rizkipriyanto Azharpratomo , Shafira Ninditya Irsan , Primanda Nur Rahmania , Sisca Meida Wati , Syafira Salsabila Adam , Kavanila Bilbalqish , Khairul Anuar bin Shariff , Harry Laksono , Agus Dahlan , Devi Rianti
Background
Tooth extraction without socket preservation will lead to reduction in the dimensions and volume of the alveolar bone. Bone defects resulting from tooth extraction not only hinder prosthetic reconstruction but also present aesthetic issues and complicating dental implant treatment. Purple leaves contains flavonoids, steroids, tannins, saponins,and non-toxic alkaloids. These compounds play roles as antimicrobials, immunomodulators, antioxidants, anti-inflammatories, analgesics. Hydroxyapatite has good biocompatibility, and can induce osteoblast differentiation.
Aim
To investigate the effects of the combining nanoemulsion extract of purple leaves and hydroxyapatite on the expression of RUNX2, OSX, OPN, ALP, and calcium deposition.
Method
This research used MTT Assay, ICC, and Alizarin Red staining. The study groups were as follows: Group 1: Ad-MSCs; Group 2: Ad-MSCs + αmem; Group 3: Ad-MSCs + osteogenic medium + 1 % nano-extract of Purple leaves & Hydroxyapatite; Group 4: Ad-MSCs + osteogenic medium + 2 % nano-extract of Purple leaves & Hydroxyapatite. Observations were conducted on days 7, 14, and 21.
Results
The combination of nanosuspension extract of Purple leaves and hydroxyapatite significantly increased the expression of RUNX2, OSX, OPN,ALP and calcium deposition compared to other groups. The combination of nanoemulsion extract of Purple leaves and hydroxyapatite were significant (P < 0.05) compared to the control group on each day 7, day 14 and day 21.
Conclusion
The combination of nanosuspension extract of Purple leaves and hydroxyapatite was able to enhance the expression of RUNX2, OSX, OPN, ALP, and calcium deposition on days 7,14,21.
{"title":"Effectiveness of purple leaves (Graptophyllum pictum L. Griff) and hydroxyapatite as socket preservation biomaterials","authors":"Ratri Maya Sitalaksmi , Rizkipriyanto Azharpratomo , Shafira Ninditya Irsan , Primanda Nur Rahmania , Sisca Meida Wati , Syafira Salsabila Adam , Kavanila Bilbalqish , Khairul Anuar bin Shariff , Harry Laksono , Agus Dahlan , Devi Rianti","doi":"10.1016/j.jobcr.2024.12.002","DOIUrl":"10.1016/j.jobcr.2024.12.002","url":null,"abstract":"<div><h3>Background</h3><div>Tooth extraction without socket preservation will lead to reduction in the dimensions and volume of the alveolar bone. Bone defects resulting from tooth extraction not only hinder prosthetic reconstruction but also present aesthetic issues and complicating dental implant treatment. Purple leaves contains flavonoids, steroids, tannins, saponins,and non-toxic alkaloids. These compounds play roles as antimicrobials, immunomodulators, antioxidants, anti-inflammatories, analgesics. Hydroxyapatite has good biocompatibility, and can induce osteoblast differentiation.</div></div><div><h3>Aim</h3><div>To investigate the effects of the combining nanoemulsion extract of purple leaves and hydroxyapatite on the expression of RUNX2, OSX, OPN, ALP, and calcium deposition.</div></div><div><h3>Method</h3><div>This research used MTT Assay, ICC, and Alizarin Red staining. The study groups were as follows: Group 1: Ad-MSCs; Group 2: Ad-MSCs + αmem; Group 3: Ad-MSCs + osteogenic medium + 1 % nano-extract of Purple leaves & Hydroxyapatite; Group 4: Ad-MSCs + osteogenic medium + 2 % nano-extract of Purple leaves & Hydroxyapatite. Observations were conducted on days 7, 14, and 21.</div></div><div><h3>Results</h3><div>The combination of nanosuspension extract of Purple leaves and hydroxyapatite significantly increased the expression of RUNX2, OSX, OPN,ALP and calcium deposition compared to other groups. The combination of nanoemulsion extract of Purple leaves and hydroxyapatite were significant (P < 0.05) compared to the control group on each day 7, day 14 and day 21.</div></div><div><h3>Conclusion</h3><div>The combination of nanosuspension extract of Purple leaves and hydroxyapatite was able to enhance the expression of RUNX2, OSX, OPN, ALP, and calcium deposition on days 7,14,21.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 63-68"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11696788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Technical insights on adjustable velar extension and safety mechanism in Tübingen palatal plate for Pierre Robin sequence","authors":"Hemwati Nandan , Bert Braumann , Teresa Kruse , Michael Wolf , Pragjyoti Jha , Srinivas Gosla Reddy , Prasad Nalabothu","doi":"10.1016/j.jobcr.2024.12.021","DOIUrl":"10.1016/j.jobcr.2024.12.021","url":null,"abstract":"","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 188-190"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Odontogenesis is a complex and highly regulated biological process that involves a range of molecular mechanisms. Among these, Ki67 and Cyclin D1 are crucial cell cycle regulators that play pivotal roles in controlling cell proliferation during tooth development. This study aims to provide detailed insights into the expression patterns and functional significance of Ki67 and Cyclin D1 in tooth development. Through rigorous analysis, we seek to elucidate the intricate mechanisms underlying tooth development, helping to advance our understanding of this vital biological process.
Materials & methods
The procurement of rabbit tooth germ was performed only after obtaining the requisite ethical clearance. Subsequently, the tissues were processed and subjected to Hematoxylin and Eosin staining to facilitate enhanced visualization of the overall tissue architecture and organization & immunohistochemical staining of Ki-67 and Cyclin D1 was performed.
Results
In the tooth germ, cyclin D1 demonstrated intense staining in the dental papilla, especially in the membrana preformativa, with this intensity decreasing following predentin formation. Odontoblasts showed mild staining as they transitioned from pre-odontoblasts, which further diminished after dentin formation. Both the dental papilla and differentiating odontoblasts were positive for Ki67, though Ki67 staining in the odontoblasts reduced after dentin formation.
Conclusion
In conclusion, the membrana preformativa plays a key role in odontogenesis, as indicated by its involvement in cellular proliferation and differentiation during tooth development.
{"title":"Membrana preformativa: Unveiling the unexplored facets of dental development","authors":"Sakshi Sikaria, Suganya Panneer Selvam, Ramya Ramadoss, Sandhya Sundar","doi":"10.1016/j.jobcr.2024.12.010","DOIUrl":"10.1016/j.jobcr.2024.12.010","url":null,"abstract":"<div><h3>Aim</h3><div>Odontogenesis is a complex and highly regulated biological process that involves a range of molecular mechanisms. Among these, Ki67 and Cyclin D1 are crucial cell cycle regulators that play pivotal roles in controlling cell proliferation during tooth development. This study aims to provide detailed insights into the expression patterns and functional significance of Ki67 and Cyclin D1 in tooth development. Through rigorous analysis, we seek to elucidate the intricate mechanisms underlying tooth development, helping to advance our understanding of this vital biological process.</div></div><div><h3>Materials & methods</h3><div>The procurement of rabbit tooth germ was performed only after obtaining the requisite ethical clearance. Subsequently, the tissues were processed and subjected to Hematoxylin and Eosin staining to facilitate enhanced visualization of the overall tissue architecture and organization & immunohistochemical staining of Ki-67 and Cyclin D1 was performed.</div></div><div><h3>Results</h3><div>In the tooth germ, cyclin D1 demonstrated intense staining in the dental papilla, especially in the membrana preformativa, with this intensity decreasing following predentin formation. Odontoblasts showed mild staining as they transitioned from pre-odontoblasts, which further diminished after dentin formation. Both the dental papilla and differentiating odontoblasts were positive for Ki67, though Ki67 staining in the odontoblasts reduced after dentin formation.</div></div><div><h3>Conclusion</h3><div>In conclusion, the membrana preformativa plays a key role in odontogenesis, as indicated by its involvement in cellular proliferation and differentiation during tooth development.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 84-87"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.jobcr.2024.12.016
Sedighe Sadat Hashemikamangar , Aidin Sooratgar , Mina Khayamzadeh , Shayan Momeni , Ali Asghar Safaei , Behnaz Behniafar
Introdution
Although oral and dental diseases may occur in unexpected or even emergency conditions, but some of the diagnosis and treatments can be algorithmically done following some guidelines. The development and implementation of a system that provides users with a record of history and a proposal of required actions can be not only efficiently practical, but also virtually simple.
Materials and method
A system made up of web and mobile apps is proposed and evaluated for screening and self-care of oral and dental problems and for providing advice on dental emergencies and therapeutic measures. This system was developed and made available to users under the name of 'Dandanyar'. The data were then collected from Dandanyar users and analyzed based on the selected statistical population for evaluating their satisfaction.
Results
The evaluation results showed that more than 90 % of specialists had a positive attitude towards application effectiveness. On the other hand, clients expressed their satisfaction with various aspects of the application in more than 80 % of cases.
Conclusion
The importance of using such systems is highlighted in the Covid-19 pandemic condition where certain health protocols must be followed. For instance, people must avoid unnecessary traveling and limit their referrals to medical centers especially dental clinics due to the high volume of aerosols produced during dental treatment procedures.
{"title":"Design and evaluation of screening and self-care (mobile) application for oral and dental problems and emergencies","authors":"Sedighe Sadat Hashemikamangar , Aidin Sooratgar , Mina Khayamzadeh , Shayan Momeni , Ali Asghar Safaei , Behnaz Behniafar","doi":"10.1016/j.jobcr.2024.12.016","DOIUrl":"10.1016/j.jobcr.2024.12.016","url":null,"abstract":"<div><h3>Introdution</h3><div>Although oral and dental diseases may occur in unexpected or even emergency conditions, but some of the diagnosis and treatments can be algorithmically done following some guidelines. The development and implementation of a system that provides users with a record of history and a proposal of required actions can be not only efficiently practical, but also virtually simple.</div></div><div><h3>Materials and method</h3><div>A system made up of web and mobile apps is proposed and evaluated for screening and self-care of oral and dental problems and for providing advice on dental emergencies and therapeutic measures. This system was developed and made available to users under the name of 'Dandanyar'. The data were then collected from Dandanyar users and analyzed based on the selected statistical population for evaluating their satisfaction.</div></div><div><h3>Results</h3><div>The evaluation results showed that more than 90 % of specialists had a positive attitude towards application effectiveness. On the other hand, clients expressed their satisfaction with various aspects of the application in more than 80 % of cases.</div></div><div><h3>Conclusion</h3><div>The importance of using such systems is highlighted in the Covid-19 pandemic condition where certain health protocols must be followed. For instance, people must avoid unnecessary traveling and limit their referrals to medical centers especially dental clinics due to the high volume of aerosols produced during dental treatment procedures.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 113-122"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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